CN206244806U - The micro- extraction systems of DNA - Google Patents
The micro- extraction systems of DNA Download PDFInfo
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- CN206244806U CN206244806U CN201621356283.4U CN201621356283U CN206244806U CN 206244806 U CN206244806 U CN 206244806U CN 201621356283 U CN201621356283 U CN 201621356283U CN 206244806 U CN206244806 U CN 206244806U
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Abstract
The utility model provides a kind of micro- extraction systems of DNA, the micro- extraction systems of DNA include extracting chip, the extraction chip includes superstructure, cation-exchange membrane and understructure, and the superstructure and understructure are sealed between cation-exchange membrane by thermocompression bonding；The system also includes anode electrode, cathode electrode and power supply.System of the present utility model carries out on-line preconcentration by online to DNA weak solutions, in enrichment process, removes electropositive and neutral substance, effectively realizes the purifying of DNA, extracts and be enriched with.
Description
Technical field
The utility model belongs to Biochemistry Experiment device, and in particular to a kind of micro- extraction systems of DNA.
Background technology
Treatment, purifying and the extraction of biological sample are to biological study important in inhibiting.Due to the biology of biological sample
Activity can be influenceed by various environmental factors, and the holding time is shorter, for needing a small amount of use extraction from biological material, it is necessary to grind
Study carefully a set of system simple to operation of exploitation or device.
In the prior art, nucleic acid substances are carried out with lock out operation usually using molecular agents box, which needs artificial behaviour
Make, the poor and speed of repeatability it is slow, the reagent type of consumption is more, and needs multi-pass operation, there is that elapsed time is long, operation
It is cumbersome, the problems such as DNA purity is inadequate.
CN201410843810.3 discloses a kind of extraction from biological material apparatus and method, including：Nucleic acid memory block, mobile phase
Memory block, chromatographic column, temperature-controlled member, DNA collecting regions, RNA collecting regions；The nucleic acid memory block and the mobile phase memory block connect
Connect the entrance of the chromatographic column；The outlet of the chromatographic column is by the connection of the switch one DNA collecting regions and by switch two
Connect the RNA collecting regions；The temperature-controlled member is wrapped in the chromatographic column.
The content of the invention
In view of the shortcomings of the prior art, the utility model provides a kind of micro- extraction systems of DNA, relative to prior art, if
Count a kind of micro- extraction chips of DNA, by carrying out on-line preconcentration to DNA weak solutions online, in enrichment process, removal electropositive and
Neutral substance, effectively realizes the purifying of DNA, extracts and be enriched with.
The utility model provides a kind of micro- extraction systems of DNA, including extracts chip, and the extraction chip is tied including upper strata
Structure, cation-exchange membrane and understructure, the superstructure and understructure by thermocompression bonding, and by cation-exchange membrane
Sealing is between.
Further, the superstructure is provided with the outlet of sample inlet, enrichment region, liquid outlet and pregnant solution, described
The outlet of sample inlet, liquid outlet and pregnant solution is connected by fluid course with enrichment region.
Further, the understructure is provided with anolyte inlet, cation-exchange membrane groove, anode pool and anolyte
Liquid outlet, the anolyte inlet is connected with anode pool by fluid course；The cation-exchange membrane groove goes out with anolyte
Liquid mouthful is connected by fluid course.
Further, the system that the utility model is provided includes anode electrode and cathode electrode, and the cathode electrode is set
In liquid outlet；The anode electrode is arranged on anolyte inlet；The anode electrode and cathode electrode are connected with power lead.
Further, the anolyte inlet is connected by constant flow pump one with anodic dissolution.
Further, the sample inlet is connected by constant flow pump two with sample solution.
Further, the anolyte liquid outlet is connected by silicone tube with discarded liquor collecting device.
Further, the liquid outlet is connected by silicone tube with discarded liquor collecting device.
Further, the pregnant solution outlet is connected by silicone tube with enrichment liquor collecting device.
Further, the constant flow pump one, constant flow pump two can realize that switching different solutions lead to by using tongs
The control in road, it would however also be possible to employ automatics realizes controlling the magnetic valve of each solution channel to realize.The constant flow pump one, constant current
Pump two realizes that it is switched by external power supply.
Further, the anodic dissolution is PBS；The sample solution is to treat that the DNA that enrichment is extracted is molten
Liquid.
Further, the power supply is connected with power switch.
Further, the length of the superstructure is less than the understructure.
Further, the superstructure is formed by the first slide and the second slide thermocompression bonding；The pregnant solution outlet
Including the outlet of the first pregnant solution and the outlet of the second pregnant solution；The outlet of the sample inlet, liquid outlet and the first pregnant solution is to set
The through hole on the first slide is put, the sample inlet connects enrichment region with liquid outlet by fluid course；The enrichment region
The second slide is arranged on the outlet of the second pregnant solution, the enrichment region and the outlet of the second pregnant solution are connected by fluid course；Institute
Enrichment region is stated for through hole；The first pregnant solution outlet is corresponding with the second pregnant solution outlet port and size, and the first enrichment
Liquid outlet is through hole, and the outlet of the second pregnant solution is hole；The fluid course is arranged on the lower surface or the second glass of the first slide
The upper surface of piece.
Further, the understructure is formed by the 3rd slide and the 4th slide thermocompression bonding；The anolyte feed liquor
Mouth includes first anode liquid inlet and second plate liquid inlet；The first anode liquid inlet and second plate liquid feed liquor
The position of mouth is corresponding with size；The anode pool includes first anode pond and second plate pond；The first anode pond and second
The position of anode pool is corresponding with size；The first anode liquid inlet, cation-exchange membrane groove, first anode pond and anolyte
Liquid outlet is the through hole being arranged on the 3rd slide；The cation-exchange membrane is arranged in cation-exchange membrane groove；The sun
Amberplex groove and anolyte liquid outlet are circulated by being arranged on the liquid of the lower surface of the 4th slide upper surface or the 3rd slide
Road is communicated；The second plate liquid inlet and second plate pond are the hole being arranged on the 4th slide, and by being arranged on
The fluid course connection of the lower surface of the 4th slide upper surface or the 3rd slide.
Further, in actual mechanical process of the present utility model, by the closing that fluid course is realized in tongs
And opening.
Further, fluid course described in the utility model is the thin layer passage of certain depth and width.This practicality is new
Power supply described in type is voltage-stabilized power supply, and the anode electrode and cathode electrode are inertia platinum electrode, and the inertia platinum electrode is consolidated
Due in hard conduit；The anode electrode and cathode electrode are realized extracting the energization in chip system by wiring conductive；Institute
Cation-exchange membrane groove is stated for installing cation-exchange membrane.
Beneficial effect：
The utility model provides a kind of micro- extraction systems of DNA, the enrichment of DNA is realized based on electrodialytic technique, so as to reach
Extract the purpose of DNA.In the utility model implementation process, the electronegativity based on DNA under electric field action, is migrated to anode region,
In transition process, due to the obstruct of cation-exchange membrane so that DNA is intercepting periphery formation enrichment, so as to realize that DNA's is micro-
Extract and purify.
System cost of the present utility model is low, simple structure, sensitivity are high, easy to operate, safeguard simple, and the system is compared to existing
There is technology, do not rely on the enrichment that manually operation is capable of achieving DNA, simple to operate, environment-friendly, effectively removal electropositive is with
Property material, automatic operating degree is high, is easy to small-scale application.
Brief description of the drawings
The structural representation of Fig. 1 the utility model preferred embodiments；
The front side structure schematic diagram of Fig. 2 the utility model preferred embodiments；
The schematic diagram of the superstructure of Fig. 3 the utility model preferred embodiments；
The schematic diagram of the understructure of Fig. 4 the utility model preferred embodiments；
The structural representation of the first slide of Fig. 5 the utility model preferred embodiments；
The structural representation of the second slide of Fig. 6 the utility model preferred embodiments；
The structural representation of the 3rd slide of Fig. 7 the utility model preferred embodiments；
The structural representation of the 4th slide of Fig. 8 the utility model preferred embodiments.
Specific embodiment
It is below in conjunction with the accompanying drawings and specific real to make those skilled in the art more fully understand the technical solution of the utility model
Mode is applied to be further described the utility model.
Embodiment 1
As shown in Fig. 1~8, the utility model provides a kind of micro- extraction systems of DNA, including power supply 1, extraction chip 2, anode
Electrode 3 and cathode electrode 4；Wherein, it is described extraction chip include superstructure, cation-exchange membrane 5 and understructure, it is described on
Rotating fields and understructure are sealed between cation-exchange membrane 5 by thermocompression bonding；The superstructure is set
There are sample inlet 21, enrichment region 22, liquid outlet 23 and pregnant solution outlet 24, the sample inlet 21, liquid outlet 23 and richness
Liquid collecting outlet 24 is connected by fluid course with enrichment region 23；The cathode electrode 4 is arranged on liquid outlet 23；Lower floor's knot
Structure is provided with anolyte inlet 25, cation-exchange membrane groove 28, anode pool 26 and anolyte liquid outlet 27, and the anolyte enters
Liquid mouthful 25 is connected with anode pool 26 by fluid course；The cation-exchange membrane groove 28 passes through liquid stream with anolyte liquid outlet 27
Passage is connected；The anode electrode 3 is arranged on anolyte inlet 25；The anode electrode 3 and cathode electrode 4 are led with power supply 1
Line is connected.
The sample inlet 21, liquid outlet 23, anolyte inlet 25, anolyte liquid outlet 27 and pregnant solution outlet 24
It is connected with silicone tube by hard conduit, tongs is provided with the silicone tube.
The anolyte inlet 25 is connected by constant flow pump one with anodic dissolution.
The sample inlet 21 is connected by constant flow pump two with sample solution.
The anolyte liquid outlet 27 is connected by silicone tube with discarded liquor collecting device.
The liquid outlet 23 is connected by silicone tube with discarded liquor collecting device.
The pregnant solution outlet 24 is connected by silicone tube with enrichment liquor collecting device.
The constant flow pump one, constant flow pump two can realize the control of switching different solutions passage by using tongs,
Can also realize controlling the magnetic valve of each solution channel to realize using automatics.The constant flow pump one, constant flow pump two lead to
Cross external power supply and realize that it is switched.
The anodic dissolution is PBS；The sample solution is to treat the DNA solution that enrichment is extracted.
The power supply 1 is connected with power switch.
The length of the superstructure is less than the understructure.
As shown in Fig. 3,5,6, the superstructure is formed by the first slide and the second slide thermocompression bonding；The pregnant solution
Outlet 24 includes that the first pregnant solution exports 24-1 and the second pregnant solution outlet 24-2；The sample inlet 21, the and of liquid outlet 23
First pregnant solution outlet 24-1 is the through hole being arranged on the first slide, and the sample inlet 21 is with liquid outlet 23 by setting
Enrichment region 22 is connected in the fluid course of the first slide lower surface；The pregnant solution of the enrichment region 22 and second outlet 24-2 is arranged on
Second slide, the pregnant solution of the enrichment region 22 and second exports 24-2 and is connected by being arranged on the fluid course of the second slide upper surface
It is logical；The enrichment region 22 is through hole；The first pregnant solution outlet 24-1 exports 24-2 positions and size phase with the second pregnant solution
Correspondence, and the first pregnant solution outlet 24-1 is through hole, the second pregnant solution outlet 24-2 is hole.
As shown in Fig. 4,7,8, the understructure is formed by the 3rd slide and the 4th slide thermocompression bonding；The anolyte
Inlet 25 includes first anode liquid inlet 25-1 and second plate liquid inlet 25-2；The first anode liquid inlet
25-1 is corresponding with size with the position of second plate liquid inlet 25-2；The anode pool 26 includes first anode pond 26-1 and the
Two anode pool 26-2；The first anode pond 26-1 is corresponding with size with the position of second plate pond 26-2；The first anode
Liquid inlet 25-1, cation-exchange membrane groove 28, first anode pond 26-1 and anolyte liquid outlet 27 are to be arranged on the 3rd slide
On through hole；The cation-exchange membrane 5 is arranged on the upper surface of cation-exchange membrane groove 28；The He of cation-exchange membrane groove 28
Anolyte liquid outlet 27 is communicated by being arranged on the fluid course of the lower surface of the 3rd slide；The second plate liquid inlet
25-2 and second plate pond 26-2 are the hole being arranged on the 4th slide, and by being arranged on the liquid of the 4th slide upper surface
Circulation road is connected.
In actual mechanical process of the present utility model, closing and the opening of fluid course are realized by tongs.
Fluid course described in the utility model is the thin layer passage of certain depth and width.Described in the utility model
Power supply 1 is voltage-stabilized power supply, and the anode electrode 3 and cathode electrode 4 are inertia platinum electrode, and the inertia platinum electrode is fixed on hard
In conduit；The anode electrode 3 and cathode electrode 4 are realized extracting the energization in the system of chip 2 by conductive；The cation is handed over
Film groove 28 is changed for installing cation-exchange membrane 5.
The course of work：
The micro- extraction systems of DNA of the utility model design are to be based on DNA for elecrtonegativity material, under electric field action, on the sunny side
Polar region is migrated, and belongs to electrodialytic technique.
Specific implementation process is：
1) anode region Job readiness：Start constant flow pump one, anodic dissolution enters the anode for extracting chip 2 by constant flow pump one
Liquid inlet 25, so as to flow to anode pool 26, after full of anode pool 26, flows out through fluid course to anolyte liquid outlet 27
Afterwards, constant flow pump one is closed；In implementation process, if desired anodic dissolution is changed, constant flow pump one can be started by being spaced
Realize.
2) cathodic region Job readiness：Pregnant solution outlet 24 is closed, starts constant flow pump two, sample solution is entered by constant flow pump two
Enter to extract the sample inlet 21 of chip 2, so as to flow to enrichment region 22, after full of after enrichment region 22, through fluid course to going out liquid
After the outflow of mouth 23, constant flow pump two is closed.
3) power-on, an electric field is formed in the both sides of cation-exchange membrane 5, keeps sample solution flowing, and sample is molten
DNA in liquid, due to electric field action, flows when enrichment region 22 is flowed through to anode electrode direction, is flowing to cation exchange
It is blocked at film 5, so as to realize the enrichment of DNA.
4) after sample solution all flows through enrichment region 22, power supply 1 is closed, suspends constant flow pump one and two, close liquid outlet
23, constant flow pump two and pregnant solution outlet 24 are opened, pregnant solution is enriched with liquor collecting device and collects concentration through the driving of constant flow pump two
DNA solution, completes micro- extraction of DNA.
It is understood that embodiment of above be merely to illustrate that principle of the present utility model and use it is exemplary
Implementation method, but the utility model is not limited thereto.For those skilled in the art, this is not being departed from
In the case of the spirit and essence of utility model, various changes and modifications can be made therein, and these variations and modifications are also considered as being wrapped
It is contained in protection domain of the present utility model.
Claims (8)
1. micro- extraction systems of a kind of DNA, it is characterised in that the micro- extraction systems of DNA include extracting chip, the extraction chip
Including superstructure, cation-exchange membrane and understructure, the superstructure and understructure by thermocompression bonding, and by sun
Amberplex is sealed between.
2. micro- extraction systems of a kind of DNA according to claim 1, it is characterised in that the superstructure is provided with sample
Inlet, enrichment region, liquid outlet and pregnant solution outlet, the sample inlet, liquid outlet and pregnant solution outlet pass through liquid stream
Passage is connected with enrichment region.
3. micro- extraction systems of a kind of DNA according to claim 2, it is characterised in that the sample inlet passes through constant current
Pump two is connected with sample solution.
4. micro- extraction systems of a kind of DNA according to claim 1, it is characterised in that the understructure is provided with anode
Liquid inlet, cation-exchange membrane groove, anode pool and anolyte liquid outlet, the anolyte inlet pass through liquid stream with anode pool
Passage is connected；The cation-exchange membrane groove is connected with anolyte liquid outlet by fluid course.
5. micro- extraction systems of a kind of DNA according to claim 4, it is characterised in that the anolyte inlet is by perseverance
Stream pump one is connected with anodic dissolution.
6. a kind of micro- extraction systems of DNA according to claim 2 or 4, it is characterised in that the micro- extraction system bags of DNA
Anode electrode and cathode electrode are included, the anode electrode and cathode electrode are connected with power lead；The cathode electrode is arranged on
Liquid outlet；The anode electrode is arranged on anolyte inlet.
7. micro- extraction systems of a kind of DNA according to claim 2, it is characterised in that the superstructure is by the first slide
Formed with the second slide thermocompression bonding；The pregnant solution outlet includes the outlet of the first pregnant solution and the outlet of the second pregnant solution；It is described
The outlet of sample inlet, liquid outlet and the first pregnant solution is the through hole being arranged on the first slide；The enrichment region and the second richness
Liquid collecting outlet is arranged on the second slide, and the enrichment region and the outlet of the second pregnant solution are connected by fluid course；The enrichment
Area is through hole, and the sample inlet connects enrichment region with liquid outlet by fluid course；First pregnant solution outlet and the
Two pregnant solution outlet ports are corresponding with size, and the outlet of the first pregnant solution is through hole, and the outlet of the second pregnant solution is hole；It is described
Fluid course is arranged on the lower surface of the first slide or the upper surface of the second slide.
8. micro- extraction systems of a kind of DNA according to claim 4, it is characterised in that the understructure is by the 3rd slide
Formed with the 4th slide thermocompression bonding；The anolyte inlet includes first anode liquid inlet and second plate liquid feed liquor
Mouthful；The first anode liquid inlet is corresponding with size with the position of second plate liquid inlet；The anode pool includes first
Anode pool and second plate pond；The first anode pond is corresponding with size with the position in second plate pond；The first anode liquid
Inlet, cation-exchange membrane groove, first anode pond and anolyte liquid outlet are the through hole being arranged on the 3rd slide；The sun
Amberplex is arranged in cation-exchange membrane groove；The cation-exchange membrane groove and anolyte liquid outlet are by being arranged on
The fluid course of the upper surface of the lower surface of three slides or the 4th slide is communicated；The second plate liquid inlet and the second sun
Pole pond is the hole being arranged on the 4th slide, and by being arranged on the lower surface of the 3rd slide or the upper surface of the 4th slide
Fluid course is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621356283.4U CN206244806U (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621356283.4U CN206244806U (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA |
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| Publication Number | Publication Date |
|---|---|
| CN206244806U true CN206244806U (en) | 2017-06-13 |
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ID=59006031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621356283.4U Expired - Fee Related CN206244806U (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754346A (en) * | 2016-12-12 | 2017-05-31 | 厦门华厦学院 | The micro- extraction systems of DNA and the micro- extracting methods of DNA |
| US11207679B2 (en) | 2018-04-13 | 2021-12-28 | Regents Of The University Of Minnesota | DNA extraction device |
-
2016
- 2016-12-12 CN CN201621356283.4U patent/CN206244806U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754346A (en) * | 2016-12-12 | 2017-05-31 | 厦门华厦学院 | The micro- extraction systems of DNA and the micro- extracting methods of DNA |
| US11207679B2 (en) | 2018-04-13 | 2021-12-28 | Regents Of The University Of Minnesota | DNA extraction device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170613 Termination date: 20171212 |
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| CF01 | Termination of patent right due to non-payment of annual fee |