CN106754346A - The micro- extraction systems of DNA and the micro- extracting methods of DNA - Google Patents
The micro- extraction systems of DNA and the micro- extracting methods of DNA Download PDFInfo
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- CN106754346A CN106754346A CN201611138068.1A CN201611138068A CN106754346A CN 106754346 A CN106754346 A CN 106754346A CN 201611138068 A CN201611138068 A CN 201611138068A CN 106754346 A CN106754346 A CN 106754346A
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- 238000004853 microextraction Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000005341 cation exchange Methods 0.000 claims abstract description 34
- 239000012528 membrane Substances 0.000 claims abstract description 33
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 77
- 239000012530 fluid Substances 0.000 claims description 30
- 239000000523 sample Substances 0.000 claims description 21
- 239000012488 sample solution Substances 0.000 claims description 16
- 238000004090 dissolution Methods 0.000 claims description 10
- 230000005684 electric field Effects 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 6
- 239000000284 extract Substances 0.000 abstract description 3
- 230000007935 neutral effect Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000004094 preconcentration Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 46
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 229920001296 polysiloxane Polymers 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000010297 mechanical methods and process Methods 0.000 description 2
- 230000005226 mechanical processes and functions Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of micro- extraction systems of DNA and the micro- extracting methods of DNA, the micro- extraction systems of DNA include extracting chip, the extraction chip includes superstructure, cation-exchange membrane and understructure, the superstructure and understructure are sealed between cation-exchange membrane by thermocompression bonding；The system also includes anode electrode, cathode electrode and power supply.System and method of the invention carry out on-line preconcentration by online to DNA weak solutions, in enrichment process, remove electropositive and neutral substance, effectively realize the purifying of DNA, extract and be enriched with.
Description
Technical field
The invention belongs to Biochemistry Experiment device, and in particular to a kind of micro- extraction systems of DNA and the micro- extracting methods of DNA.
Background technology
Treatment, purifying and the extraction of biological sample are to biological study important in inhibiting.Due to the biology of biological sample
Activity can be influenceed by various environmental factors, and the holding time is shorter, for needing a small amount of use extraction from biological material, it is necessary to grind
Study carefully a set of system simple to operation of exploitation or device.
In the prior art, nucleic acid substances are carried out with lock out operation usually using molecular agents box, which needs artificial behaviour
Make, the poor and speed of repeatability it is slow, the reagent type of consumption is more, and needs multi-pass operation, there is that elapsed time is long, operation
It is cumbersome, the problems such as DNA purity is inadequate.
The content of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of micro- extraction systems of DNA and the micro- extracting methods of DNA, relative to
Prior art, designs a kind of micro- extraction chips of DNA, by carrying out on-line preconcentration to DNA weak solutions online, in enrichment process,
Removal electropositive and neutral substance, effectively realize the purifying of DNA, extract and be enriched with.
The present invention provides a kind of micro- extraction systems of DNA, including extracts chip, and the extraction chip includes superstructure, sun
Amberplex and understructure, the superstructure and understructure are sealed cation-exchange membrane by thermocompression bonding
In between.
Further, the superstructure is provided with the outlet of sample inlet, enrichment region, liquid outlet and pregnant solution, described
The outlet of sample inlet, liquid outlet and pregnant solution is connected by fluid course with enrichment region.
Further, the understructure is provided with anolyte inlet, cation-exchange membrane groove, anode pool and anolyte
Liquid outlet, the anolyte inlet is connected with anode pool by fluid course；The cation-exchange membrane groove goes out with anolyte
Liquid mouthful is connected by fluid course.
Further, the system that the present invention is provided includes anode electrode and cathode electrode, and the cathode electrode is arranged on out
Liquid mouthful；The anode electrode is arranged on anolyte inlet；The anode electrode and cathode electrode are connected with power lead.
Further, the anolyte inlet is connected by constant flow pump one with anodic dissolution.
Further, the sample inlet is connected by constant flow pump two with sample solution.
Further, the anolyte liquid outlet is connected by silicone tube with discarded liquor collecting device.
Further, the liquid outlet is connected by silicone tube with discarded liquor collecting device.
Further, the pregnant solution outlet is connected by silicone tube with enrichment liquor collecting device.
Further, the constant flow pump one, constant flow pump two can realize that switching different solutions lead to by using tongs
The control in road, it would however also be possible to employ automatics realizes controlling the magnetic valve of each solution channel to realize.The constant flow pump one, constant current
Pump two realizes that it is switched by external power supply.
Further, the anodic dissolution is PBS；The sample solution is to treat that the DNA that enrichment is extracted is molten
Liquid.
Further, the power supply is connected with power switch.
Further, the length of the superstructure is less than the understructure.
Further, the superstructure is formed by the first slide and the second slide thermocompression bonding；The pregnant solution outlet
Including the outlet of the first pregnant solution and the outlet of the second pregnant solution；The outlet of the sample inlet, liquid outlet and the first pregnant solution is to set
The through hole on the first slide is put, the sample inlet connects enrichment region with liquid outlet by fluid course；The enrichment region
The second slide is arranged on the outlet of the second pregnant solution, the enrichment region and the outlet of the second pregnant solution are connected by fluid course；Institute
Enrichment region is stated for through hole；The first pregnant solution outlet is corresponding with the second pregnant solution outlet port and size, and the first enrichment
Liquid outlet is through hole, and the outlet of the second pregnant solution is hole；The fluid course is arranged on the lower surface or the second glass of the first slide
The upper surface of piece.
Further, the understructure is formed by the 3rd slide and the 4th slide thermocompression bonding；The anolyte feed liquor
Mouth includes first anode liquid inlet and second plate liquid inlet；The first anode liquid inlet and second plate liquid feed liquor
The position of mouth is corresponding with size；The anode pool includes first anode pond and second plate pond；The first anode pond and second
The position of anode pool is corresponding with size；The first anode liquid inlet, cation-exchange membrane groove, first anode pond and anolyte
Liquid outlet is the through hole being arranged on the 3rd slide；The cation-exchange membrane is arranged in cation-exchange membrane groove；The sun
Amberplex groove and anolyte liquid outlet are circulated by being arranged on the liquid of the lower surface of the 4th slide upper surface or the 3rd slide
Road is communicated；The second plate liquid inlet and second plate pond are the hole being arranged on the 4th slide, and by being arranged on
The fluid course connection of the lower surface of the 4th slide upper surface or the 3rd slide.
Further, in actual mechanical process of the invention, by tongs realizing the closing of fluid course and beating
Open.
Further, fluid course of the present invention is the thin layer passage of certain depth and width.It is heretofore described
Power supply be voltage-stabilized power supply, the anode electrode and cathode electrode are inertia platinum electrode, and the inertia platinum electrode is fixed on hard
In conduit；The anode electrode and cathode electrode are realized extracting the energization in chip system by wiring conductive；The cation
Exchange membrane groove is used to install cation-exchange membrane.
On the other hand, the present invention provides a kind of micro- extracting methods of DNA, using the micro- extraction system realities of DNA of the present invention
Existing, detailed process is as follows：
1) anode region Job readiness：Start constant flow pump one, anodic dissolution enters the anode for extracting chip by constant flow pump one
Liquid inlet, so as to flow to anode pool, after full of anode pool, after being flowed out through fluid course to anolyte liquid outlet, closes permanent
Stream pump one；
2) cathodic region Job readiness：Pregnant solution outlet is closed, starts constant flow pump two, sample solution is entered by constant flow pump two
The sample inlet of chip is extracted, so as to flow to enrichment region, after full of enrichment region, after being flowed out through fluid course to liquid outlet,
Close constant flow pump two；
3) power-on, an electric field is formed in the both sides of cation-exchange membrane, keeps sample solution flowing, sample solution
In DNA when enrichment region is flowed through, due to electric field action, flowed to anode electrode direction, flowing at cation-exchange membrane
It is blocked, so as to realize the enrichment of DNA；
4) after sample solution all flows through enrichment region, power supply is closed, suspends constant flow pump one and two, close liquid outlet, beaten
Open constant flow pump two and pregnant solution outlet, pregnant solution through constant flow pump two driving, enrichment liquor collecting device collect concentration DNA it is molten
Liquid, completes micro- extraction of DNA.
Beneficial effect：
The present invention provides a kind of micro- extraction systems of DNA, the enrichment of DNA is realized based on electrodialytic technique, so as to reach extraction
The purpose of DNA.In implementation process of the present invention, the electronegativity based on DNA under electric field action, is migrated to anode region, is being migrated
Cheng Zhong, due to the obstruct of cation-exchange membrane so that DNA forms enrichment intercepting periphery, so as to realize micro- extraction of DNA and pure
Change.
The present invention provides a kind of micro- extracting methods of DNA, realizes effective purifying and extraction of DNA, simple to operate, sensitivity
Height, it is easy to operate, and the method is simple and environment-friendly.
System cost of the invention is low, simple structure, sensitivity are high, easy to operate, safeguard simple, and the system compares existing skill
Art, does not rely on the enrichment that artificial operation is capable of achieving DNA, simple to operate, environment-friendly, effectively removal electropositive and neutrals
Matter, automatic operating degree is high, is easy to small-scale application.
Brief description of the drawings
The structural representation of Fig. 1 preferred embodiment of the present invention；
The front side structure schematic diagram of Fig. 2 preferred embodiment of the present invention；
The schematic diagram of the superstructure of Fig. 3 preferred embodiment of the present invention；
The schematic diagram of the understructure of Fig. 4 preferred embodiment of the present invention；
The structural representation of the first slide of Fig. 5 preferred embodiment of the present invention；
The structural representation of the second slide of Fig. 6 preferred embodiment of the present invention；
The structural representation of the 3rd slide of Fig. 7 preferred embodiment of the present invention；
The structural representation of the 4th slide of Fig. 8 preferred embodiment of the present invention.
Specific embodiment
To make those skilled in the art more fully understand technical scheme, below in conjunction with the accompanying drawings and specific embodiment party
The invention will be further described for formula.
Embodiment 1
As shown in Fig. 1~8, the present invention provides a kind of micro- extraction systems of DNA, including power supply 1, extraction chip 2, anode electrode
3 and cathode electrode 4；Wherein, the extraction chip includes superstructure, cation-exchange membrane 5 and understructure, the upper strata knot
Structure and understructure are sealed between cation-exchange membrane 5 by thermocompression bonding；The superstructure is provided with sample
Product inlet 21, enrichment region 22, liquid outlet 23 and pregnant solution outlet 24, the sample inlet 21, liquid outlet 23 and pregnant solution
Outlet 24 is connected by fluid course with enrichment region 23；The cathode electrode 4 is arranged on liquid outlet 23；The understructure sets
It is equipped with anolyte inlet 25, cation-exchange membrane groove 28, anode pool 26 and anolyte liquid outlet 27, the anolyte inlet
25 are connected with anode pool 26 by fluid course；The cation-exchange membrane groove 28 passes through fluid course with anolyte liquid outlet 27
Connection；The anode electrode 3 is arranged on anolyte inlet 25；The anode electrode 3 and cathode electrode 4 connect with the wire of power supply 1
Connect.
The sample inlet 21, liquid outlet 23, anolyte inlet 25, anolyte liquid outlet 27 and pregnant solution outlet 24
It is connected with silicone tube by hard conduit, tongs is provided with the silicone tube.
The anolyte inlet 25 is connected by constant flow pump one with anodic dissolution.
The sample inlet 21 is connected by constant flow pump two with sample solution.
The anolyte liquid outlet 27 is connected by silicone tube with discarded liquor collecting device.
The liquid outlet 23 is connected by silicone tube with discarded liquor collecting device.
The pregnant solution outlet 24 is connected by silicone tube with enrichment liquor collecting device.
The constant flow pump one, constant flow pump two can realize the control of switching different solutions passage by using tongs,
Can also realize controlling the magnetic valve of each solution channel to realize using automatics.The constant flow pump one, constant flow pump two lead to
Cross external power supply and realize that it is switched.
The anodic dissolution is PBS；The sample solution is to treat the DNA solution that enrichment is extracted.
The power supply 1 is connected with power switch.
The length of the superstructure is less than the understructure.
As shown in Fig. 3,5,6, the superstructure is formed by the first slide and the second slide thermocompression bonding；The pregnant solution
Outlet 24 includes that the first pregnant solution exports 24-1 and the second pregnant solution outlet 24-2；The sample inlet 21, the and of liquid outlet 23
First pregnant solution outlet 24-1 is the through hole being arranged on the first slide, and the sample inlet 21 is with liquid outlet 23 by setting
Enrichment region 22 is connected in the fluid course of the first slide lower surface；The pregnant solution of the enrichment region 22 and second outlet 24-2 is arranged on
Second slide, the pregnant solution of the enrichment region 22 and second exports 24-2 and is connected by being arranged on the fluid course of the second slide upper surface
It is logical；The enrichment region 22 is through hole；The first pregnant solution outlet 24-1 exports 24-2 positions and size phase with the second pregnant solution
Correspondence, and the first pregnant solution outlet 24-1 is through hole, the second pregnant solution outlet 24-2 is hole.
As shown in Fig. 4,7,8, the understructure is formed by the 3rd slide and the 4th slide thermocompression bonding；The anolyte
Inlet 25 includes first anode liquid inlet 25-1 and second plate liquid inlet 25-2；The first anode liquid inlet
25-1 is corresponding with size with the position of second plate liquid inlet 25-2；The anode pool 26 includes first anode pond 26-1 and the
Two anode pool 26-2；The first anode pond 26-1 is corresponding with size with the position of second plate pond 26-2；The first anode
Liquid inlet 25-1, cation-exchange membrane groove 28, first anode pond 26-1 and anolyte liquid outlet 27 are to be arranged on the 3rd slide
On through hole；The cation-exchange membrane 5 is arranged on the upper surface of cation-exchange membrane groove 28；The He of cation-exchange membrane groove 28
Anolyte liquid outlet 27 is communicated by being arranged on the fluid course of the lower surface of the 3rd slide；The second plate liquid inlet
25-2 and second plate pond 26-2 are the hole being arranged on the 4th slide, and by being arranged on the liquid of the 4th slide upper surface
Circulation road is connected.
In actual mechanical process of the invention, closing and the opening of fluid course are realized by tongs.
Fluid course of the present invention is the thin layer passage of certain depth and width.Heretofore described power supply 1 is
Voltage-stabilized power supply, the anode electrode 3 and cathode electrode 4 are inertia platinum electrode, and the inertia platinum electrode is fixed in hard conduit；
The anode electrode 3 and cathode electrode 4 are realized extracting the energization in the system of chip 2 by conductive；The cation-exchange membrane groove
28 are used to install cation-exchange membrane 5.
The course of work：
The micro- extraction systems of DNA of present invention design are to be based on DNA for elecrtonegativity material, under electric field action, to anode region
Migration, belongs to electrodialytic technique.
Specific implementation process is：
1) anode region Job readiness：Start constant flow pump one, anodic dissolution enters the anode for extracting chip 2 by constant flow pump one
Liquid inlet 25, so as to flow to anode pool 26, after full of anode pool 26, flows out through fluid course to anolyte liquid outlet 27
Afterwards, constant flow pump one is closed；In implementation process, if desired anodic dissolution is changed, constant flow pump one can be started by being spaced
Realize.
2) cathodic region Job readiness：Pregnant solution outlet 24 is closed, starts constant flow pump two, sample solution is entered by constant flow pump two
Enter to extract the sample inlet 21 of chip 2, so as to flow to enrichment region 22, after full of after enrichment region 22, through fluid course to going out liquid
After the outflow of mouth 23, constant flow pump two is closed.
3) power-on, an electric field is formed in the both sides of cation-exchange membrane 5, keeps sample solution flowing, and sample is molten
DNA in liquid, due to electric field action, flows when enrichment region 22 is flowed through to anode electrode direction, is flowing to cation exchange
It is blocked at film 5, so as to realize the enrichment of DNA.
4) after sample solution all flows through enrichment region 22, power supply 1 is closed, suspends constant flow pump one and two, close liquid outlet
23, constant flow pump two and pregnant solution outlet 24 are opened, pregnant solution is enriched with liquor collecting device and collects concentration through the driving of constant flow pump two
DNA solution, completes micro- extraction of DNA.
Embodiment 2
Instantiation：It is concentration object with 10mL concentration as the DNA solution (TE10 ×, pH 7.8) of 0.3ng/ μ L：1) open
Dynamic constant flow pump one, anodic dissolution enters the anolyte inlet for extracting chip by constant flow pump one, so as to flow to anode pool, under making
The anode pool of Rotating fields is full of anode buffer liquid (1mmol/L KH2PO4+3.5mmol/L K2HPO4+1mmol/L Na2SO4, use
NaOH regulations pH is 9.0), to stop constant flow pump one；2) pregnant solution outlet is closed, starts constant flow pump two, DNA dilutions pass through constant current
Pump two enters the sample inlet for extracting chip, and so as to flow to enrichment region, the enrichment region for being full of DNA dilutions circulates through liquid
After road flows out to liquid outlet, constant flow pump two is closed；3) startup power supply operating voltage 250V, it is permanent with the flow control of 0.5mL/min
Stream pump two, makes DNA dilutions all flow through the enrichment region of superstructure, and in the process, the DNA in solution is in electric field action
Under, migrated to anode region, and enrichment is piled up on cation-exchange membrane；In the process can be by intermittently powering on constant flow pump
One realizes anode fluid exchange.4) treat that DNA dilutions have all flowed, close voltage-stabilized power supply, suspend constant flow pump two and, close out liquid
Mouthful, pregnant solution outlet is opened, start constant flow pump two, enrichment liquor collecting device collects the DNA pregnant solutions of outflow, obtains high concentration
DNA solution, detects its DNA concentration value about 300ng/ μ L.Experiment terminates, and is led to NaOH weak solutions and deionized water cleaning chip
Road.
It is understood that the embodiment of above principle being intended to be merely illustrative of the present and the exemplary implementation for using
Mode, but the invention is not limited in this.For those skilled in the art, essence of the invention is not being departed from
In the case of god and essence, various changes and modifications can be made therein, and these variations and modifications are also considered as being comprised in of the invention
In protection domain.
Claims (9)
1. micro- extraction systems of a kind of DNA, it is characterised in that the micro- extraction systems of DNA include extracting chip, the extraction chip
Including superstructure, cation-exchange membrane and understructure, the superstructure and understructure by thermocompression bonding, and by sun
Amberplex is sealed between.
2. micro- extraction systems of a kind of DNA according to claim 1, it is characterised in that the superstructure is provided with sample
Inlet, enrichment region, liquid outlet and pregnant solution outlet, the sample inlet, liquid outlet and pregnant solution outlet pass through liquid stream
Passage is connected with enrichment region.
3. micro- extraction systems of a kind of DNA according to claim 2, it is characterised in that the sample inlet passes through constant current
Pump two is connected with sample solution.
4. micro- extraction systems of a kind of DNA according to claim 1, it is characterised in that the understructure is provided with anode
Liquid inlet, cation-exchange membrane groove, anode pool and anolyte liquid outlet, the anolyte inlet pass through liquid stream with anode pool
Passage is connected；The cation-exchange membrane groove is connected with anolyte liquid outlet by fluid course.
5. micro- extraction systems of a kind of DNA according to claim 4, it is characterised in that the anolyte inlet is by perseverance
Stream pump one is connected with anodic dissolution.
6. a kind of micro- extraction systems of DNA according to claim 2 and 4, it is characterised in that the micro- extraction system bags of DNA
Anode electrode and cathode electrode are included, the anode electrode and cathode electrode are connected with power lead；The cathode electrode is arranged on
Liquid outlet；The anode electrode is arranged on anolyte inlet.
7. micro- extraction systems of a kind of DNA according to claim 2, it is characterised in that the superstructure is by the first slide
Formed with the second slide thermocompression bonding；The pregnant solution outlet includes the outlet of the first pregnant solution and the outlet of the second pregnant solution；It is described
The outlet of sample inlet, liquid outlet and the first pregnant solution is the through hole being arranged on the first slide；The enrichment region and the second richness
Liquid collecting outlet is arranged on the second slide, and the enrichment region and the outlet of the second pregnant solution are connected by fluid course；The enrichment
Area is through hole, and the sample inlet connects enrichment region with liquid outlet by fluid course；First pregnant solution outlet and the
Two pregnant solution outlet ports are corresponding with size, and the outlet of the first pregnant solution is through hole, and the outlet of the second pregnant solution is hole；It is described
Fluid course is arranged on the lower surface of the first slide or the upper surface of the second slide.
8. micro- extraction systems of a kind of DNA according to claim 4, it is characterised in that the understructure is by the 3rd slide
Formed with the 4th slide thermocompression bonding；The anolyte inlet includes first anode liquid inlet and second plate liquid feed liquor
Mouthful；The first anode liquid inlet is corresponding with size with the position of second plate liquid inlet；The anode pool includes first
Anode pool and second plate pond；The first anode pond is corresponding with size with the position in second plate pond；The first anode liquid
Inlet, cation-exchange membrane groove, first anode pond and anolyte liquid outlet are the through hole being arranged on the 3rd slide；The sun
Amberplex is arranged in cation-exchange membrane groove；The cation-exchange membrane groove and anolyte liquid outlet are by being arranged on
The fluid course of the upper surface of the lower surface of three slides or the 4th slide is communicated；The second plate liquid inlet and the second sun
Pole pond is the hole being arranged on the 4th slide, and by being arranged on the lower surface of the 3rd slide or the upper surface of the 4th slide
Fluid course is connected.
9. a kind of micro- extracting methods of DNA, detailed process is as follows：
1) anode region Job readiness：Start constant flow pump one, anodic dissolution is entered by the anolyte that constant flow pump one enters extraction chip
Liquid mouthful, so as to flow to anode pool, after full of anode pool, after being flowed out through fluid course to anolyte liquid outlet, closes constant flow pump
One；
2) cathodic region Job readiness：Closing pregnant solution outlet, starts constant flow pump two, and sample solution enters extraction by constant flow pump two
The sample inlet of chip, so as to flow to enrichment region, after full of enrichment region, after being flowed out through fluid course to liquid outlet, closes
Constant flow pump two；
3) power-on, an electric field is formed in the both sides of cation-exchange membrane, sample solution flowing is kept, in sample solution
DNA, due to electric field action, flows when enrichment region is flowed through to anode electrode direction, is hindered flowing at cation-exchange membrane
Gear, so as to realize the enrichment of DNA；
4) after sample solution all flows through enrichment region, power supply is closed, suspends constant flow pump one and two, close liquid outlet, open permanent
Stream pump two and pregnant solution outlet, pregnant solution are enriched with the DNA solution that liquor collecting device collects concentration through the driving of constant flow pump two, complete
Into micro- extraction of DNA.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138068.1A CN106754346A (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA and the micro- extracting methods of DNA |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138068.1A CN106754346A (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA and the micro- extracting methods of DNA |
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| CN106754346A true CN106754346A (en) | 2017-05-31 |
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ID=58875406
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| CN201611138068.1A Pending CN106754346A (en) | 2016-12-12 | 2016-12-12 | The micro- extraction systems of DNA and the micro- extracting methods of DNA |
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