CN206188790U - Laser -guide micro -fluidic chip to cell - Google Patents
Laser -guide micro -fluidic chip to cell Download PDFInfo
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- CN206188790U CN206188790U CN201621229701.3U CN201621229701U CN206188790U CN 206188790 U CN206188790 U CN 206188790U CN 201621229701 U CN201621229701 U CN 201621229701U CN 206188790 U CN206188790 U CN 206188790U
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- cell collection
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Abstract
The utility model relates to a laser -guide micro -fluidic chip to cell is in this chip the last passageway and lower path connection of cross passageway, as the buffer solution passageway, the prepass of cross passageway is the cell liquid passageway, and the back passageway of cross passageway forms the branched passage that four ray forms are connected as main entrance, the terminal separation of main entrance, and optic fibre imbeds the 80um department lower part before the main passage end perpendicularly. The utility model discloses a micro -fluidic chip structural design is ingenious, and the function is clear and definite, the handling ease, and work efficiency or screening efficiency improve by a wide margin.
Description
Technical field
The utility model is related to biomedical technology, changes thin sideways against cell specifically using the trapping stiffness of laser
The micro-fluidic chip of the movement locus of born of the same parents.
Background technology
Early in early in the twentieth century, biology, the scientific research personnel of medical domain have begun to carry out the work such as cell culture.For
Can more intuitively observation of cell active situation in vivo, people start the analogue body inner cell under more open environment
Required living environment.The appearance of micro-fluidic chip promotes the flow of research of external emulation with development, due to micro-fluidic chip
The characteristics of with high accuracy, high flux and strong mouldability, according to circumstances can go out application with different process and material manufacture
In the micro-fluidic chip of different field.
In recent years, the concern due to people to circulating tumor cell, to the demand of the sorting of circulating tumor cell also in day
Benefit increases, and high flux and sorting index high are needed in scientific research and clinical practice.Although existing several method separative efficiency is high,
It is more complicated from manufacture craft, or need to pre-process sample, so as to more or less pollute sample.
Recent year turns into study hotspot by means such as optical pressure, optical tweezers come microtechnique cell.Li Yinmei etc.
People and Zhang Wenjing et al. realize and are captured with optical tweezers, manipulate and separate cell, and have studied coefficient of viscosity in different liquids
Influence to it；Zhao Enming and Liu Zhihai have been realized in the work such as capture and ejection to granules of polystyrene and yeast cells
With, and probed into mechanical function of the trapping stiffness to different size particulate.But using accurate micro-shifting platform by optical tweezer perpendicular to
Chip direction migratory cell is inefficient, is not suitable for high-throughout cell screening；Captured with high power laser needs with ejection
High power laser, it is also possible to influence cytoactive.Also be not implemented in micro-fluidic chip using small lateral trapping stiffness come
Change cell track.
Utility model content
In view of the shortcomings of the prior art, the technical problem that the utility model is intended to solve is to provide one kind can make target cell
The micro-fluidic chip that movement locus deflects, the embedded optical fiber of introducing can reduce the requirement to laser power, and actual behaviour
Simplify operating procedure (setting up light path without scene) in work, relative to being published in before《Micro-nano electronic technology》Described in upper paper
Chip structure, this time using the more preferable multimode fibre of compatibility, and has carried out structure according to emulation and actual conditions to passage
Optimization, adds optical fiber embedded location, is easy to operation.The chip has flux high, unmarked, the features such as be suitable to practical application.
The technical solution of the utility model is,
A kind of laser guide micro-fluidic chip for cell, the chip include cross passage, top waste fluid channel, No. 1
Cell collection channel, No. 2 cell collection channels, bottom waste fluid channel, upper waste liquid pool, No. 1 cell harvestor, No. 2 cells are collected
Device, lower waste liquid pool and optical fiber；
Upper channel and the lower channel connection of described cross passage, as buffer solution passage；The prepass of cross passage is
Cell liquid passage, the rear passage of cross passage as main channel, the end of main channel separate to be formed the connection of four aplysia punctatas point
Subchannel, wherein, four branched bottoms are followed successively by top waste fluid channel, No. 1 cell collection channel, No. 2 cells and receive from top to bottom
Collection passage, bottom waste fluid channel, angle is 30 ° between each passage, wherein, No. 2 cell collection channels are in the water of main channel
Flat extension；Bottom at 80um before fiber perpendicular insertion main channel end；
The end of top waste fluid channel be upper waste liquid pool, the end of No. 1 cell collection channel be No. 1 cell harvestor, No. 2
The end of cell collection channel be No. 2 cell harvestors, bottom waste fluid channel end be lower waste liquid pool；
Described cross passage, top waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste liquids
Passage material is dimethyl silicone polymer.
Described cross passage, top waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste liquids
The inner passage width of passage is equal, and channel thickness is equal.
Described cross passage, top waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste liquids
The inner passage width of passage is 100 μm, and channel thickness is 100 μm.
Compared with prior art, microfluidic chip structure of the present utility model designs ingenious, definite functions, is easy to processing,
Operating efficiency or screening efficiency are greatly improved up to several-tens/seconds, and need not use any reagent label target
Cell, is truly realized unmarked, high-throughout cell screening modern medicine requirement.
Brief description of the drawings
Fig. 1 is the utility model chip structure schematic diagram.
Specific embodiment
The utility model is further described with reference to embodiment and its accompanying drawing.
Microfluidic chip structure needed for this experiment is designed as shown in Fig. 1, which includes the most frequently used cross passage work
It is sheath flow structure, and has innovated the multiple-limb channel design of uniqueness, is easy to the screening and classification of cell.The chip includes：It is slow
Fliud flushing passage (1), cell liquid passage (2), cross passage (3), main channel (4), top waste fluid channel (5), No. 1 cell collect logical
Road (6), No. 2 cell collection channels (7), bottom waste fluid channel (8), upper waste liquid pool (9), No. 1 cell harvestor (10), No. 2 it is thin
Born of the same parents' collector (11), lower waste liquid pool (12) and optical fiber (14)；Its cross passage (3) (including main channel (4)) top waste fluid channel
(5), No. 1 cell collection channel (6), No. 2 cell collection channels (7), bottom waste fluid channel (8) design width it is equal, make
When channel thickness it is also equal；
Upper channel and the lower channel connection of described cross passage (3), as buffer solution passage；Before cross passage (3)
Passage (2) is cell liquid passage, and used as main channel, the end of main channel separates and to form four and penetrate the rear passage (4) of cross passage
The branched bottom of wire connection, belongs to same node altogether, wherein, four branched bottoms are followed successively by top waste fluid channel from top to bottom
(5), No. 1 cell collection channel (6), No. 2 cell collection channels (7), bottom waste fluid channels (8), angle is between each passage
30 °, wherein, No. 2 horizontal extension sections of the cell collection channel (7) in main channel (4)；Fiber perpendicular insertion main channel end it
At preceding 80um；
The end of top waste fluid channel (5) is upper waste liquid pool (9), the end of No. 1 cell collection channel (6) is No. 1 cell
Collector (10), the end of No. 2 cell collection channels (7) are No. 2 cell harvestors (11), the ends of bottom waste fluid channel (8)
It is lower waste liquid pool (12).
Embodiment 1
In the utility model, the upper channel and lower channel connection (being square) of described cross passage (3), as buffer solution
Passage；The prepass (2) of cross passage (3) is cell liquid passage, and the rear passage (4) of cross passage is used as main channel (main channel
24mm long), the end of main channel separates the branched bottom to form the connection of four aplysia punctatas, i.e., No. 1 cell collection channel (6), No. 2
Cell collection channel (7), top waste fluid channel (5) and bottom waste fluid channel (8) left end access the tail end of main channel (4) jointly,
The horizontal extensions section of wherein No. 2 cell collection channels (7) in main channel (4), and No. 1 cell collection channel (6), No. 2 it is thin
Born of the same parents' collection channel (7), the diameter of top waste fluid channel (5) and bottom waste fluid channel (8) and main channel (4) equal diameters, are
100 μm, channel thickness is also 100 μm, and angle is 30 ° between each passage, and four tail ends of path of the above correspond to No. 1 respectively
Cell harvestor (10), No. 2 cell harvestors (11), upper waste liquid pool (9) and lower waste liquid pools (12).In order to be able in micro-fluidic chip
Middle embedded optical fiber, this experiment uses dimethyl silicone polymer (PDMS) as the main making material of chip, i.e. cross passage
(3), top waste fluid channel (5), No. 1 cell collection channel (6), No. 2 cell collection channels (7), bottom waste fluid channel (8) materials
It is dimethyl silicone polymer.The passage of the reserved optical fiber (14) size (fibre diameter 125um) of correspondence position (13) in template
(at the 80um before fiber perpendicular insertion main channel end), makes at its end face perpendicular alignmnet fork road front passage.Then insert
Enter after optical fiber with the gap between PDMS injection fibres and passage, and reinforced with silica gel and make at optical fiber and micro-fluidic chip interface
Optical fiber forms entirety with chip.The operation principle and process of the utility model chip be：Cross passage (3) is easier to make from buffering
The buffer solution of liquid passage (1) and cell liquid passage (2) injection and it is buffered the cell liquid that liquid is wrapped in and converges to form sheath stream, and
The flow velocity and pressure of buffer solution passage (1) are controlled by controlling the flow velocity of injector, it is ensured that cell can be inner in main channel (4)
Form unicellular stream, and direction along streamline enters the irradiation that optical fiber embedded location (13) is received through the laser of optical fiber,
Due to different cells different cell membrane and cytoplasm, so for the lower suffered power of the laser irradiation of different wave length, power
Also it is different, so as to realize screening the cell of variety classes, size, allow that axial trapping stiffness is produced by adjusting power
Raw 0-40 μm transversal displacement, it is sufficient to by cell separation to different passages.Enter final by the cell of cell screening
No. 1 collection channel (6) of cell and No. 2 cell collection channels (7), complete have high flux, unmarked cell screening process.
The utility model does not address part and is applied to prior art.
Claims (3)
1. a kind of laser guide micro-fluidic chip for cell, it is characterized by the chip includes that cross passage, top waste liquid lead to
Road, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste fluid channel, upper waste liquid pool, No. 1 cell harvestor, No. 2 it is thin
Born of the same parents' collector, lower waste liquid pool and optical fiber；
Upper channel and the lower channel connection of described cross passage, as buffer solution passage；The prepass of cross passage is cell
Liquid passage, used as main channel, the end of main channel separates and to form the branch of four aplysia punctatas connection and lead to the rear passage of cross passage
Road, wherein, four branched bottoms are followed successively by top waste fluid channel, No. 1 cell collection channel, No. 2 cells and collect logical from top to bottom
Road, bottom waste fluid channel, angle is 30 ° between each passage, wherein, level of No. 2 cell collection channels in main channel is prolonged
Section long；Bottom at 80 μm before fiber perpendicular insertion main channel end；
The end of top waste fluid channel is that upper waste liquid pool, the end of No. 1 cell collection channel are No. 1 cell harvestor, No. 2 cells
The end of collection channel be No. 2 cell harvestors, bottom waste fluid channel end be lower waste liquid pool；
Described cross passage, top waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste fluid channels
Material is dimethyl silicone polymer.
2. the laser guide micro-fluidic chip for cell as claimed in claim 1, it is characterized by described cross passage, on
Portion's waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, bottom waste fluid channel inner passage width it is equal, lead to
Road thickness is equal.
3. the laser guide micro-fluidic chip for cell as claimed in claim 1, it is characterized by described cross passage, on
Portion's waste fluid channel, No. 1 cell collection channel, No. 2 cell collection channels, the inner passage width of bottom waste fluid channel are 100 μ
M, channel thickness is 100 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621229701.3U CN206188790U (en) | 2016-11-16 | 2016-11-16 | Laser -guide micro -fluidic chip to cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621229701.3U CN206188790U (en) | 2016-11-16 | 2016-11-16 | Laser -guide micro -fluidic chip to cell |
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| Publication Number | Publication Date |
|---|---|
| CN206188790U true CN206188790U (en) | 2017-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621229701.3U Expired - Fee Related CN206188790U (en) | 2016-11-16 | 2016-11-16 | Laser -guide micro -fluidic chip to cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468026A (en) * | 2019-09-07 | 2019-11-19 | 桂林电子科技大学 | A kind of micro flow chip for optical fiber light power cell operation |
| CN110468027A (en) * | 2019-09-07 | 2019-11-19 | 桂林电子科技大学 | A kind of cell sorting micro flow chip based on coaxial double wave guiding fiber |
-
2016
- 2016-11-16 CN CN201621229701.3U patent/CN206188790U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468026A (en) * | 2019-09-07 | 2019-11-19 | 桂林电子科技大学 | A kind of micro flow chip for optical fiber light power cell operation |
| CN110468027A (en) * | 2019-09-07 | 2019-11-19 | 桂林电子科技大学 | A kind of cell sorting micro flow chip based on coaxial double wave guiding fiber |
| CN110468027B (en) * | 2019-09-07 | 2022-04-19 | 桂林电子科技大学 | Cell sorting microfluidic chip based on coaxial double-waveguide optical fiber |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170524 Termination date: 20171116 |