CN107085292B - A kind of light control techniques for red blood cell controllable rotating and double optical fiber optical tweezers of deformation - Google Patents
A kind of light control techniques for red blood cell controllable rotating and double optical fiber optical tweezers of deformation Download PDFInfo
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Abstract
The present invention provides a kind of production method for red blood cell controllable rotating and double optical fiber optical tweezers of deformation, the method is double optical fiber optical tweezers to be prepared by fused biconical taper method, and have the pyramidal structure of gradual change in end.The controllable rotating of human erythrocyte is realized simultaneously using the shoot laser of double-tapered fiber optical tweezer through the invention, including around x-axis, the rotation manipulation of z-axis and diversification.The invention also achieves unicellular and many cells light-operated deformations.
Description
Technical field
The invention belongs to field of photoelectric technology, and in particular to it is a kind of using double optical fiber optical tweezers realize red blood cell controllable rotatings and
The light control techniques of deformation.
Background technique
It is well known that red blood cell plays suitable important function during organism metabolism and aerobic respiration,
Physics and mechanistic nature are also taken as the important indicator of biomedical and Clinico Pathologic research.Realize the accurate behaviour of red blood cell
Control, especially controllable rotating and deformation, are conducive to further understand eucaryotic cell structure in micro/nano-scale, to the diagnosis of vascular diseases, layer
Analysis micro-imaging, the driving of cell micromechanics, the conduction of mechanism power and chemical substance release etc. are all of great significance.Up to now,
It has been proposed that kinds of schemes is used for the controllable rotating of red blood cell, including dielectrophoresis, dual-beam spanner and focusing micro-imaging technique
Deng.Meanwhile for red blood cell controllable deforming, researcher is also by microfiltration technology, laser diffraction technology, micro-pipe conveying, electric field
And atomic force microscopy etc. is achieved.In addition, also there is research to catch by using holographic optical tweezer or based on non-rotational symmetry
Traditional optical tweezer of light beam and annular polarization light beam is obtained to realize the controllable rotating manipulation of red blood cell.
Summary of the invention
In view of this, above technical scheme is only limitted to single functionization operation, i.e., it can only realize that the rotation of cell or cell become
Shape.For the physics and mechanistic nature of eased effective research red blood cell, the controllable of cell is realized based on single platform simultaneously
Rotation and deformation are just increasingly causing the close attention of researcher.And for traditional optical tweezer and holographic optical tweezer method, once
Light arresting structure determines that cell can only be around the axial-rotation being pre-designed.Since optical fiber optical tweezers itself have, production is simple, is convenient for
The characteristics such as integrated, flexible operation, the shoot laser for further utilizing double-tapered fiber optical tweezer is realized that human body is red thin by us simultaneously
The controllable rotating of born of the same parents, including around x-axis, the rotation manipulation of z-axis and diversification.In addition, can also be achieved based on the technical solution slender
The light-operated deformation of born of the same parents and many cells.
It is described i.e. the present invention provides a kind of production method for red blood cell controllable rotating and double optical fiber optical tweezers of deformation
Method is double optical fiber optical tweezers to be prepared by fused biconical taper method, and have the pyramidal structure of gradual change in end.
Preferably, the present invention is used in the production method of double optical fiber optical tweezers of red blood cell controllable rotating and deformation, including with
Lower step:
1) two single mode optical fibers are removed into buffer layer and polymer-coated layer respectively, and put on glass capillary；
2) optical fiber of two step 1) removal buffer layers and polymer-coated layer is placed in oxyhydrogen flame and is heated to optical fiber welding
It is stretched after point along optical axis direction；
3) two optical fiber will break into two parts, and form the pyramidal structure of gradual change in end.
Preferably, the present invention is used in the production method of double optical fiber optical tweezers of red blood cell controllable rotating and deformation, the step
It is rapid 2) in be 0.5mm/s to the initial tensile speed of two optical fiber.
Preferably, the present invention is used in the production method of double optical fiber optical tweezers of red blood cell controllable rotating and deformation, the step
It is rapid 2) in fibre diameter reduce to 7.8 μm within the scope of 1.8-2.2mm from 125 μm first, then from 7.8 μ out of 5.6-6.4 μ m
M reduces to 0.47-0.53 μm and (refers to that optical fiber pointed cone front end portion diameter is decreased to 0.47-0.53 μm from 7.8 μm, corresponding laterally length
Degree is to change between 5.6-6.4 μm, similarly hereinafter), tensile speed is mentioned to 2mm/s, optical fiber optical tweezers will fracture and form spy in end
Fixed conical by its shape；
Preferably, fibre diameter reduces to 7.8 μm from 125 μm first within the scope of 1.8-2.2mm, from 7.8 in 6 μm of length
When μm reducing to 0.5 μm, tensile speed is mentioned to 2mm/s, optical fiber optical tweezers will fracture and form specific conical by its shape in end.
It is another aspect of the invention to provide the above methods to obtain double optical fiber optical tweezers, and double optical fiber optical tweezers are by molten
Melt and draw the preparation of cone method, and there is the pyramidal structure of gradual change in end.
Preferably, in double optical fiber optical tweezers that the method for the invention obtains, the pyramidal structure of double optical fiber optical tweezers ends
It is 0.5mm/s for the initial tensile speed to two optical fiber；When fibre diameter subtracts within the scope of 1.8-2.2mm from 125 μm first
0.47-0.53 μm is reduced to from 7.8 μm to 7.8 μm, then out of 5.6-6.4 μ m, tensile speed is mentioned to 2mm/s, optical fiber light
Tweezer will fracture and form specific conical by its shape in end；
Preferably, fibre diameter reduces to 7.8 μm from 125 μm first within the scope of 1.8-2.2mm, from 7.8 in 6 μm of length
When μm reducing to 0.5 μm, tensile speed is mentioned to 2mm/s, optical fiber optical tweezers will fracture and form specific conical by its shape in end.
The double optical fiber optical tweezers obtained the present invention also provides the above method are including following to the method for red blood cell controllable rotating
Step:
1) preparation double optical fiber optical tweezers as described above, are connected respectively to the output end of two lasers, will contain red blood cell
The glass slide of solution is placed in x-y and manually adjusts platform；
It 2), will after being passed through laser to the first optical fiber optical tweezers by the first optical fiber optical tweezers close to the red blood cell in solution of red blood cells
Red blood cell is captured to the front end of the first optical fiber optical tweezers, closes laser after steady contact；
3) it is passed through laser to the second optical fiber optical tweezers, manipulates the captured red blood cell of the first optical fiber optical tweezers, passes through the second optical fiber light
Tweezer is translated along-y/+y axis direction, to rotate captured red blood cell；
Wherein, the wavelength of first optical fiber optical tweezers is 980nm, power 30mW；
The wavelength of second optical fiber optical tweezers is 980nm, power 24mW.
Preferably, double optical fiber optical tweezers of the present invention are in the method for red blood cell controllable rotating, the method also includes
Second optical fiber optical tweezers are passed through laser, manipulate the captured red blood cell of the first optical fiber optical tweezers, by the second optical fiber optical tweezers along the axis side-y/+y
To translation, so that captured red blood cell is rotated around z-axis.
Preferably, double optical fiber optical tweezers of the present invention are in the method for red blood cell controllable rotating, the method also includes
Second optical fiber optical tweezers are passed through laser, the left-half of the captured red blood cell of the first optical fiber optical tweezers is manipulated, passes through the second optical fiber light
Tweezer is translated along-y/+y axis direction, so that captured red blood cell is rotated around I axis, and realizes the diversification rotation of captured red blood cell.
Invention further provides double optical fiber optical tweezers of the present invention to the method for red blood cell controllable deforming, including following step
It is rapid:
1) the double optical fiber optical tweezers obtained such as the above method are prepared, the output end of two lasers is connected respectively to, will contain
The glass slide of solution of red blood cells is placed in x-y and manually adjusts platform；
2) by the first optical fiber optical tweezers, the second optical fiber optical tweezers respectively close to the both ends of the red blood cell in solution of red blood cells, simultaneously
It is passed through laser, red blood cell will be captured under photodynamic action and arrange along optical fiber optical tweezers axial direction, and along optical fiber optical tweezers axial direction
Direction stretcher strain；
Wherein, the red blood cell is one or more red blood cells；
The wavelength of the laser is 980nm, and power can be adjusted according to the degree of RBC deformation.
Therefore the present invention at least has the advantage that
1. optical fiber optical tweezers of the invention have, production is simple, manipulates the advantages such as flexible, non-contact and not damaged capture, thus
Complicated photolithographic substrates production and the processing of body material micro-nano needed for remaining technical solution are avoided, in the biological micro-pipe that blood vessel etc. is narrow
There is potential important application in manipulation；
2. in the inventive solutions, it is not necessarily to be confined to substrate surface in cell tandem tissue and alignment processes, from
And potential cell sample is avoided to pollute, and the recycling of feasible system；
3. double optical fiber optical tweezers systems of the invention can be in low cell especially suitable for the cell solution under low concentration
In the cell solution of concentration, the controllable adjustment of cell tandem is realized；
4. double optical fiber optical tweezers systems of the invention can be achieved at the same time the rotation and deformation manipulation of red blood cell, that is, realize red thin
Born of the same parents are around x-axis, the rotation manipulation of z-axis and diversification.In addition, can also be achieved unicellular and many cells light based on the technical solution
Control deformation；
5. the tapered end face of optical fiber optical tweezers can realize outgoing beam strong-focusing, so that laser power needed for cytomorphosis be subtracted
To tens of milliwatts, the potential optical damage for cell is avoided；
6. technical solution of the present invention avoids complicated Holographic Algorithm design, and can realize the dynamic adjustment of cell tandem.
Since optical fiber optical tweezers itself are integrated and the advantage of micromation is expected to by further combining it with micro flow chip equipment
For cell growth, the processes such as tissue differentiation, intercellular signal transmitting provide strong research tool.
Detailed description of the invention
Fig. 1 is that the present invention is based on the schematic illustrations that double optical fiber optical tweezers realize red blood cell rotation and deformation；Wherein, Fig. 1 a is
Red blood cell rotates around x axis schematic diagram, Fig. 1 b is red blood cell geometric representation, and Part III shows the left-half of red blood cell；
Fig. 2 is double optical fiber optical tweezers experimental provision schematic diagrams in one embodiment of the present of invention；
Fig. 3 is that double optical fiber optical tweezers realize the optical microphotograph picture that red blood cell rotates around x axis in one embodiment of the present of invention,
Lateral arrows indicate that optical direction, longitudinal arrow indicate cell direction of rotation, scale: 5 μm；
Fig. 4 is that double optical fiber optical tweezers realize that red blood cell rotates schematic diagram around z-axis；
Fig. 5 is that double optical fiber optical tweezers realize the optical microphotograph signal that red blood cell is rotated around z-axis in one embodiment of the present of invention
Figure；
Fig. 6 is the optical microscopy map that double optical fiber optical tweezers realize red blood cell diversification rotation in one embodiment of the present of invention
Piece；
Fig. 7 is that double optical fiber optical tweezers realize the optical microscopy map that two red blood cells deform simultaneously in one embodiment of the present of invention
Piece；
Fig. 8 is that double optical fiber optical tweezers realize the optical microscopy map that three red blood cells deform simultaneously in one embodiment of the present of invention
Piece；
Fig. 9 is the optical microphotograph picture that double optical fiber optical tweezers realize Shan Hong cytomorphosis in one embodiment of the present of invention, mark
Ruler: 5 μm.
Specific embodiment
Mentality of designing of the invention is as follows:
Red blood cell rotates schematic diagram, the effect based on luminous power and Van der Waals for around conical fiber optical tweezer as shown in Figure 1:
Red blood cell one end can be fixed on to the tip of the first optical fiber optical tweezers of taper (lower abbreviation TFP1)；At this point, to the first optical fiber optical tweezers (under
Abbreviation TFP2) in be passed through the laser of certain power and wavelength after, the top half of red blood cell will be in the effect of 2 shoot laser of TFP
It is lower captured, and then moved with TFP 2 in the direction y.It, will be by this time since red blood cell one end is fixed on 1 tip TFP
It is rotated to the effect of luminous power square, and around optical fiber optical tweezers axial (x-axis).
Based on above-mentioned mentality of designing, provided in one embodiment of the present of invention a kind of for red blood cell controllable rotating and change
The production method of double optical fiber optical tweezers of shape, the method are double optical fiber optical tweezers to be prepared by fused biconical taper method, and have in end
The pyramidal structure of gradual change.
Preferably, in one embodiment of the invention, double optical fiber light for red blood cell controllable rotating and deformation
The production method of tweezer, comprising the following steps:
1) two single mode optical fibers are removed into buffer layer and polymer-coated layer respectively, and put on glass capillary；
2) optical fiber of two step 1) removal buffer layers and polymer-coated layer is placed in oxyhydrogen flame and is heated to optical fiber welding
It is stretched after point along optical axis direction；
3) two optical fiber will break into two parts, and form the pyramidal structure of gradual change in end.
Preferably, in one embodiment of the invention, it is to the initial tensile speed of two optical fiber in the step 2)
0.5mm/s。
Preferably, in one embodiment of the invention, double optical fiber of the present invention for red blood cell controllable rotating and deformation
In the production method of optical tweezer, fibre diameter reduces to 7.8 μm from 125 μm within the scope of 1.8-2.2mm first in the step 2), then
0.47-0.53 μm is reduced to from 7.8 μm out of 5.6-6.4 μ m, tensile speed is mentioned to 2mm/s, optical fiber optical tweezers will fracture simultaneously
Specific conical by its shape is formed in end；
Preferably, fibre diameter reduces to 7.8 μm from 125 μm first within the scope of 1.8-2.2mm, from 7.8 in 6 μm of length
When μm reducing to 0.5 μm, tensile speed is mentioned to 2mm/s, optical fiber optical tweezers will fracture and form specific conical by its shape in end.
It is highly preferred that in another embodiment of the present invention, providing the above method and obtaining double optical fiber optical tweezers, double light
Fine optical tweezer is to be prepared by fused biconical taper method, and have the pyramidal structure of gradual change in end.
It is highly preferred that in another embodiment of the present invention, the pyramidal structure of double optical fiber optical tweezers ends is to two
The initial tensile speed of a optical fiber is 0.5mm/s；When fibre diameter reduces to 7.8 μ from 125 μm first within the scope of 1.8-2.2mm
M, then reduce to 0.47-0.53 μm from 7.8 μm out of 5.6-6.4 μ m and (refer to that optical fiber pointed cone front end portion diameter subtracts from 7.8 μm
As low as 0.47-0.53 μm, corresponding lateral length is to change between 5.6-6.4 μm), tensile speed is mentioned to 2mm/s, optical fiber light
Tweezer will fracture and form specific conical by its shape in end；
Preferably, fibre diameter reduces to 7.8 μm from 125 μm within the scope of 1.8-2.2mm first, then out of 6 μm length from
7.8 μm when reducing to 0.5 μm, tensile speed are mentioned to 2mm/s, optical fiber optical tweezers will fracture and form specific cone shape in end
Shape.
It is highly preferred that in yet another embodiment of the present invention, additionally providing double optical fiber optical tweezers pair of above method acquisition
The method of red blood cell controllable rotating, comprising the following steps:
1) preparation double optical fiber optical tweezers as described above, are connected respectively to the output end of two lasers, will contain red blood cell
The glass slide of solution is placed in x-y and manually adjusts platform；
It 2), will after being passed through laser to the first optical fiber optical tweezers by the first optical fiber optical tweezers close to the red blood cell in solution of red blood cells
Red blood cell is captured to the front end of the first optical fiber optical tweezers, closes laser after steady contact；
3) it is passed through laser to the second optical fiber optical tweezers, manipulates the captured red blood cell of the first optical fiber optical tweezers, passes through the second optical fiber light
Tweezer is translated along-y/+y axis direction, to rotate captured red blood cell；
Wherein, the wavelength of first optical fiber optical tweezers is 980nm, power 30mW；
The wavelength of second optical fiber optical tweezers is 980nm, power 24mW.
It is highly preferred that in yet another embodiment of the present invention, double optical fiber optical tweezers of the present invention are controllable to red blood cell
In the method for rotation, the method also includes the second optical fiber optical tweezers to be passed through laser, manipulates the captured red blood cell of the first optical fiber optical tweezers,
It is translated by the second optical fiber optical tweezers along-y/+y axis direction, so that captured red blood cell is rotated around z-axis.
It is highly preferred that in yet another embodiment of the present invention, double optical fiber optical tweezers of the present invention are controllable to red blood cell
In the method for rotation, the method also includes the second optical fiber optical tweezers are passed through laser, the first optical fiber optical tweezers of manipulation capture red thin
The left-half of born of the same parents is translated by the second optical fiber optical tweezers along-y/+y axis direction, so that captured red blood cell is rotated around I axis, and it is real
The now diversification rotation of captured red blood cell.
It is highly preferred that providing double optical fiber optical tweezers of the present invention to red thin in yet another embodiment of the present invention
The method of born of the same parents' controllable deforming, comprising the following steps:
1) the double optical fiber optical tweezers obtained such as the above method are prepared, the output end of two lasers is connected respectively to, will contain
The glass slide of solution of red blood cells is placed in x-y and manually adjusts platform；
2) by the first optical fiber optical tweezers, the second optical fiber optical tweezers respectively close to the both ends of the red blood cell in solution of red blood cells, simultaneously
It is passed through laser, red blood cell will be captured under photodynamic action and arrange along optical fiber optical tweezers axial direction, and along optical fiber optical tweezers axial direction
Direction stretcher strain；
Wherein, the red blood cell is one or more red blood cells；
The wavelength of the laser is 980nm, and power can be adjusted according to the degree of RBC deformation.
Further technical solution of the present invention is illustrated below by way of specific embodiment, it should be understood that be below only this hair
Bright exemplary illustration, is not intended to restrict the invention scope of protection of the claims.
Production method of the embodiment 1 for the double optical fiber optical tweezers and its experimental provision of red blood cell controllable rotating and deformation
It prepares double optical fiber optical tweezers and its experimental provision is as shown in Figure 2:
One, optical fiber optical tweezers FP 1 and FP2 is prepared by flame fused biconical taper method:
First by single mode optical fiber (connection type: FC/PC, optical fiber core diameter: 9 μm, cladding diameter: 125 μm；Corning
Inc. buffer layer) and polymer coating optical fiber pincers strip, then by outside of fiber put on a capillary glass tube (internal diameter:
~0.9mm, pipe thickness :~0.1mm, pipe range :~120mm) to protect optical fiber not to be damaged and be bent.By stretched portion as
Heating about 1 minute above alcolhol burner flame applies one to the optical fiber being heated after heating region temperature reaches optical fiber fusing point
Initial tensile, tensile speed 0.5mm/s.At the same time, fibre diameter will be decreased to 7.8 μ from 125 μm in the length of 2mm
M forms the taper of a gradual change.And then, fibre diameter reduces to 0.5 μm from 7.8 μm in 6 μm of length.Finally, by that will draw
It stretches speed to mention to 2mm/s, optical fiber optical tweezers will fracture and form specific conical by its shape in end.
Two, the production method for red blood cell controllable rotating and double optical fiber optical tweezers experimental provisions of deformation
After optical fiber optical tweezers make, it is connected to one and is connected to the optical microscopy of CCD for observing and remembering in real time
Record.As shown in Fig. 2, being placed on fine adjustment frame 1 and 2, and be connected to laser 1 after conical fiber optical tweezer makes
With 2 output end.The allocation plan of red blood cell suspension is as follows: taking 20 μ L blood from the adult man finger tip of a health and collects
In the sterile anti-coagulants added with glucose and sodium citrate.After being further centrifuged with 2500 revs/min of speed, by upper layer
Blood plasma and leucocyte removal.Then purified red blood cell is placed in phosphate buffer, diluted concentration to 3 × 104A/
mL.After suspension configures, dripped by syringe in glass slide.Fine adjustment frame 1 and 2 is adjusted, so that optical fiber optical tweezers
End is placed in red blood cell suspension.Filling the glass slide of red blood cell suspension, be placed on can be in the load that x-y plane manually adjusts
On object platform (precision: 50nm), to realize preferable position precision and mechanism stability.In addition to this, using integrated CCD in experiment
Optical microscopy, carry out picture catching and video record, and whole process can carry out in real time on coupled computer
Monitoring.
According to theory analysis above it is found that before cell rotation process, red blood cell one end need to be fixed on optical fiber optical tweezers
The end of TFP 1.In this experiment, we realize the above operation based on the collective effect of luminous power and Van der Waals for: hot
Cell suspending liquid drips after in glass slide, and adjusting fine adjustment frame makes optical fiber optical tweezers TFP 1 close to specific red blood cell.It connects
, the laser (power P: 30mW) that wavelength is 980nm is passed through into optical fiber optical tweezers TFP 1, cell will be under optical gradient forces effect
It is caught into optical fiber optical tweezers front end, and is in contact with its front-end surface.After about 3min is steadily contacted, laser is closed, at this time cell
The front end of TFP 1 will be fixed in the case where Van der Waals for acts on.
2 pairs of optical fiber optical tweezers experimental provisions of embodiment are to red blood cell controllable rotating and deformation experiment
1, double optical fiber optical tweezers manipulation red blood cells rotate around x axis experiment
Specific experiment manipulation is as shown in Figure 3: injecting 980nm laser (power P to the end of optical fiber optical tweezers TFP 22=
After 24mW), the controllable rotating operation of red blood cell is as shown in Figure 3: in t=0s, a red blood cell is fixed on optical fiber optical tweezers TFP 1
Front end, squint angle be θ=0 °.It is translated with optical fiber optical tweezers TFP 2 along the direction-y, cell will be rotated around x-axis counterclockwise therewith
(as shown by arrows), and θ is consequently increased.As optical fiber optical tweezers TFP 2 remains stationary (t=8-10s, such as Fig. 3 a5~a6 institute
Show), cell is stablized simultaneously in the position of θ=172 °.Further, it adjusts TFP 2 to translate along the direction+y, θ will be gradually reduced and most
Restore eventually to (Fig. 3 a7-a9) at initial θ=0 °.Note: scale: 5 μm.
2, double optical fiber optical tweezers manipulation red blood cells are around z-axis rotation test
As shown in figure 4, if adjusting optical fiber optical tweezers TFP 2 in the z-direction, making cell centre when cell orientation angle θ=90 °
It is aligned in the direction z (that is, z with optical axisTFP2=zRBC=0 μm), the Part III of cell will be under the action of luminous power
It is captured by optical fiber optical tweezers TFP 2；At this point, translating in the y-direction with optical fiber optical tweezers TFP 2, cell will be rotated around z-axis.
Further, we realize that red blood cell rotates manipulation experiment around z-axis, as shown in Figure 5: as t=0s, manipulating optical fiber light
Tweezer TFP 2 captures red blood cell left-half；It is moved with TFP 2 along the direction-y, cell is rotated by 90 ° (such as Fig. 5 a-e counterclockwise therewith
It is shown).Further along the direction-y moving fiber optical tweezer TFP 2, cell will be disengaged from the capture of optical fiber optical tweezers TFP 2, and gradually adjust
Axial direction of the whole orientation along TFP 1 (as shown in Fig. 5 f-i).
3, the diversification rotation of double optical fiber optical tweezers manipulation red blood cells
Further by accurately manipulating fine adjustment frame, it can be achieved that the diversification of red blood cell rotates manipulation, as shown in Figure 6: vertical
The direction of rotation of cell is indicated to arrow.In t=0s, cell orientation angle is θ=90 °, and optical fiber optical tweezers TFP 2 is located at cell
Lower section (Fig. 6 a).After being passed through laser into TFP 2, the upper half of cell will be captured, thus red blood cell will be therewith around x-axis
Rotation, while θ increases to 120 ° (Fig. 6 b) by 90 °.Optical fiber optical tweezers TFP 2 is translated along the direction+y, θ will be gradually decreased therewith at this time
To 90 ° (Fig. 6 c).Optical fiber optical tweezers TFP 2 is adjusted along the direction-z at this time, so that optical fiber optical tweezers optical axis is in the direction z and cell centre weight
It closes.Continue to translate TFP 2 along the direction+y, red blood cell will be rotated around z-axis, and θ simultaneously245 ° (Fig. 6 d) gradually will be increased to from 0 °.
TFP 2 is further adjusted, cell can be rotated by 90 ° (Fig. 6 e, f) around specific axis I (as shown in dotted line in 6e).Continue along the direction+y
Moving fiber optical tweezer, cell will be rotated around z-axis (as shown in arrow longitudinal in Fig. 6 g).Finally, maintaining 2 position TFP solid
Fixed, cell will also be remain stationary (Fig. 6 h), scale: 5 μm.
4 pairs of optical fiber optical tweezers manipulate multiple red blood cells controllable deforming simultaneously
One, to the controllable deforming of 2 red blood cells
Optical fiber optical tweezers TFP 1 and 2 is adjusted first and is located at the both ends for being steered cell, while after being passed through 980nm laser,
Red blood cell will be captured under photodynamic action and arrange along optical fiber optical tweezers axial direction.Further, under photic stress, quilt
The red blood cell of capture will be along optical fiber optical tweezers axial direction stretcher strain, and deformation extent can pass through manipulation laser power and cone of light
The spacing of tweezer to red blood cell is adjusted, as shown in Figure 7: being adjusted two optical fiber optical tweezers first and is located at two red blood cell two sides
The spacing of (cell dia is respectively 5.6 and 6.4 μm) and red blood cell is 2 μm.Then, being passed through wavelength into two optical fiber optical tweezers is
Laser (the P of 980nm1=P2=20mW), red blood cell will be moved toward one another and be in contact with each other under the action of luminous power.At the same time, carefully
Born of the same parents will gradually be stretched and reach stable state when t=10s.Cell tensile degree can be characterized with tangential strain (γ),
Its definition are as follows: γ=Δ l/l, wherein Δ l and l is respectively the deflection and the preceding former length of deformation of cell.When thin in above-mentioned experiment
When born of the same parents reach stable state (Fig. 7 c), the tangential strain value that two cells are calculated is respectively 0.14 and 0.12.As t=35s
(Fig. 7 d) closes incident laser, and cell will be gradually brought to initial state at this time, such as Fig. 7 e, shown in f.
Two, to multiple red blood cell controllable deformings
In addition to two red blood cells, deformation while we also achieve three and multiple red blood cells, as shown in Figure 8: in t=
When 0s, three red blood cells (diameter is respectively 6.7,5.7 and 6.9 μm) are located among two optical fiber optical tweezers.When into optical fiber optical tweezers
After being passed through laser, red blood cell will be captured and be stretched (Fig. 8 b) along optical axis direction.In t=5s, three red blood cells finally reach
To stable state, and deformation quantity is respectively 0.15,0.1 and 0.12 (Fig. 8 c).In t=9s, laser is closed, red blood cell at this time will
It is gradually brought to original shape (Fig. 8 d-f).
Three, to single celled controllable deforming
In addition to deforming while multiple red blood cells, we also achieve single celled controllable deforming, as shown in Figure 9: in t=
When 0s, red blood cell is located among two optical fiber optical tweezers TFP 1 and 2 (Fig. 9 a).In t=3s, laser is passed through into optical fiber optical tweezers,
Red blood cell will be stretched (Fig. 9 b) under photodynamic action at this time, and progressively reach an equilibrium state (Fig. 9 c), and hereafter cell will
Deformation is kept until laser shutdown.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of utilize double optical fiber optical tweezers to the method for red blood cell controllable rotating, which comprises the following steps:
1) double optical fiber optical tweezers are connected respectively to the output end of two lasers, and the glass slide containing solution of red blood cells is placed in x-
Y manually adjusts platform；
It 2), will be red thin after being passed through laser to the first optical fiber optical tweezers by the first optical fiber optical tweezers close to the red blood cell in solution of red blood cells
Born of the same parents capture to the front end of the first optical fiber optical tweezers, close laser after steady contact；
3) it is passed through laser to the second optical fiber optical tweezers, manipulates the captured red blood cell of the first optical fiber optical tweezers, pass through the second optical fiber optical tweezers edge-
Y/+y axis direction translation, to rotate captured red blood cell；
Wherein, the wavelength of first optical fiber optical tweezers is 980nm, power 30mW；
The wavelength of second optical fiber optical tweezers is 980nm, power 24mW；
The production methods of double optical fiber optical tweezers the following steps are included:
1) two single mode optical fibers are removed into buffer layer and polymer-coated layer respectively, and put on glass capillary；
2) optical fiber of two steps 1) removal buffer layers and polymer-coated layer is placed in after oxyhydrogen flame is heated to optical fiber fusing point
It is stretched along optical axis direction；Initial tensile speed to two optical fiber is 0.5mm/s；
3) fibre diameter reduces to 7.8 μm from 125 μm within the scope of 1.8-2.2mm first, then from 7.8 μ out of 5.6-6.4 μ m
M reduces to 0.47-0.53 μm, and tensile speed is mentioned to 2mm/s, and optical fiber optical tweezers will fracture and form specific cone shape in end
Shape.
2. the method according to claim 1 to red blood cell controllable rotating, which is characterized in that the method also includes second
Optical fiber optical tweezers are passed through laser, manipulate the captured red blood cell of the first optical fiber optical tweezers, flat along-y/+y axis direction by the second optical fiber optical tweezers
It moves, so that captured red blood cell is rotated around z-axis.
3. the method according to claim 1 to red blood cell controllable rotating, which is characterized in that the method also includes by
Two optical fiber optical tweezers are passed through laser, manipulate the left-half of the captured red blood cell of the first optical fiber optical tweezers, pass through the second optical fiber optical tweezers edge-
The translation of y/+y axis direction so that captured red blood cell is rotated around I axis, and realizes the diversification rotation of captured red blood cell.
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| CN108517295A (en) * | 2018-06-01 | 2018-09-11 | 东莞东阳光科研发有限公司 | A kind of cell capture device |
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| CN111007028A (en) * | 2019-12-31 | 2020-04-14 | 暨南大学 | Preparation method of optical fluid biological waveguide, visible light transmission method and method for detecting red blood cell backscattering signal |
| CN113514441A (en) * | 2021-07-12 | 2021-10-19 | 桂林电子科技大学 | Optical sheet fluorescence microscopy method and system based on cell rotation active light control technology |
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