CN206132715U - Development of collection separation method , online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph - Google Patents
Development of collection separation method , online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph Download PDFInfo
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- CN206132715U CN206132715U CN201620666461.7U CN201620666461U CN206132715U CN 206132715 U CN206132715 U CN 206132715U CN 201620666461 U CN201620666461 U CN 201620666461U CN 206132715 U CN206132715 U CN 206132715U
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Abstract
The utility model provides a development of collection separation method, online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph, belongs to crude drug composition separation technical field, is applied to traditional chinese medicine, autonomic drug, marine drug's etc. Research and development field. This chromatograph includes control system, pump income system, sampling system, detecting system, one -dimensional chromatographic column system, two -dimentional chromatographic column system and your eight logical valves. Realize separation method development and optimization, crude drug's two -dimentional quadrature chromatographic fractionation preparation on same platform instrument. Separate into 18 components that can repeatedly obtain or effective position with the natural products of complicacy, the two dimension separation makes its further separation obtain monomer compound, and overall mask work work under computer control, very big improvement crude drug such as traditional chinese medicine systematicness separation and preparation's efficiency, for the preparation of traditional chinese medicine, autonomic drug, the effective position of marine drug, composition separates, activity screening provides the high efficiency, reliable platform.
Description
Technical field
This utility model is related to the natural drug two dimension system of a kind of collection separation method exploitation, ON-LINE SEPARATION-be enriched in one
Standby chromatograph, belongs to natural medicinal ingredients separation technology field, is applied to the research and development field of plant amedica, Chinese medicine, marine drug etc..
Background technology
Chromatographic separation technology prepares the natural product from plant, marine organisms, Chinese medicine and biological engineering etc. for separation
The importance studied for original new drug, fine chemicals is not housed doubts.Based on Chinese medicine Development of New Drugs and Chinese medicines
The discovery of reason active component, the key of study mechanism are prepared by repeatable, the systematic separation of Effective Component of Chinese Medicine.
Natural drug composition is complicated, and single chromatographic isolation effect is limited, needs, through separating for several times, to make in separation process
Satisfactory result can be reached with different chromatographic isolation patterns.During conventional Natural products research, although have accumulated rich
Rich experience, but for the natural medicinal ingredients such as systematic Study Chinese medicine still have shortage effectively equipment.In recent years, Two way chromatograms
The development of technology, using several modes filler complementarity separate undoubtedly for solve this problem paved road.And incite somebody to action
It realizes automatization, is realizing that position separates and pure compound is directly carried out after carrying out on-line preconcentration prepare that separation system can be simplified
Standby process, improves to greatest extent work efficiency.Shao Ping（CN101791491A）Devise a set of basic, normal, high pressure is combined two
Dimension preparing chromatography system, is coupled together two sets of preparing chromatography systems by six-way valve and software, completes the Two way chromatograms of sample
Separate, be suitable for separating principal component and for solving the contradiction between overload sample introduction and separating power, be not not suitable for Chinese medicine etc.
Complex system.Zhang Weibing devises a kind of Quasi dynamic two dimension preparing chromatography system and material separation method（CN102914610A,
CN202870047U）, two preparative hplc posts are connected by three-way valve, preparative hplc post separation is tieed up by first by Vavle switching
Component be transferred to the second dimension preparative hplc post and separated.Li Heran, Xiao Hongbin are disclosed（CN102961892A、
CN104713973A）Using 2 10 port valves and the side of the on-line preconcentration chromatograph separating natural ingredient of multiple 2 six-way valves
Method.Solve problem to a certain extent, but in separation method exploitation, separation efficiency and the repeatable effective site, effectively of obtaining
Deficiency is still suffered from the systematicness separation at position and the Automated condtrol of separation process.
The content of the invention
To solve problems of the prior art, this utility model provide a kind of collection separation method exploitation, ON-LINE SEPARATION-
The natural drug two dimension preparative chromatograph and its method of work of one are enriched in, the device is to Chinese medicine, plant amedica, marine drug
The intellectualizing system that systematicness, scale and serialization separate preparation is carried out Deng complex material system, sample point is effectively being carried out
Develop with the basis of optimization from method, using computer controls, prepared by the separation for being transitioned into sample, increased separation efficiency and
Specific aim, the design of example enrichment pattern, drastically increases and prepares color after one-dimensional, Two way chromatograms orthogonality separation and separation
Spectrum repeats effective site, the effective ingredient monomer for obtaining for the separation preparative capacibility of the complex material system such as Chinese medicine,
For new Chinese medicine exploitation, pharmacology activity research is significant.
The technical solution adopted in the utility model is：A kind of collection separation method exploitation, the day of ON-LINE SEPARATION-be enriched in one
Right medicine two dimension preparative chromatograph, it includes control system, it is characterised in that：It includes pumping into system, sampling system, detection system
System, one-dimensional chromatogram column system, Two way chromatograms column system and two eight ways valves, two eight ways valves include A mouths, B mouths, C mouths, D
Mouth, E mouths, F mouths, G mouths and H mouths；Including the first pump, the second pump and blender, blender is connected with to pump into and is the system that pumps into
System input pipe；The sampling system includes sampling system input pipe, two six-way injection valves, sampling system outlet tubes；The inspection
Examining system includes detecting system input pipe, the clematis stem post selector valve arm of clematis stem post selector valve, detector, the threeway for setting gradually
Blender and detecting system outlet tube；The one-dimensional chromatographic column piece-rate system is using 5 chromatographs for being connected to clematis stem post selector valve
One of post be sequentially connected as detached dowel, again one-dimensional chromatographic column input pipe, the one-dimensional ten through posts selector valve of two series connection, 18 one
Dimension chromatograph enrichment post and one-dimensional chromatographic column outlet tube；The Two way chromatograms column system is made using one of 18 one-dimensional chromatograph enrichment posts
For loading post, be connected to one of 5 chromatographic columns of clematis stem post selector valve be sequentially connected as detached dowel, again Two way chromatograms post input
Pipe, two-dimentional ten through posts selector valve and 9 Two way chromatograms enriching columns and fraction collector；Diluting pump is connected to threeway blender；
Control system and the first pump, the second pump, diluting pump, two eight ways valves, two six-way injection valves, clematis stem post selector valve, detections
The one-dimensional ten through posts selector valve of instrument, two-dimentional ten through posts selector valve are electrically connected, and control system controls the first pump, the second pump and dilution
The unlatching and stopping of liquid pump, control system controls two eight ways valves, two six-way injection valves, clematis stem post selector valve, one-dimensional ten and leads to
The break-make switching of post selector valve, two-dimentional ten through posts selector valve；
The chromatograph is by the connection of two eight ways valves of control in the first connection status or the second connection status；
First connection status is divided into the one-dimensional chromatographic isolation state of sample separation method development status and sample；Sample
Separation method development status are connected to pump into system input pipe with A mouths, and Jing B mouths are connected with sampling system input pipe, C mouths and sample introduction
System outlet tube connects, and D mouths are connected with detecting system input pipe, and the clematis stem post selector valve arm of clematis stem post selector valve enters detection
System, Jing threeway blender, detecting system outlet tube, into after G mouths by the one-dimensional chromatographic column input pipes of H mouth Jing, one-dimensional chromatographic column
HW highway and one-dimensional chromatographic column outlet tube enter F mouths, and Jing E mouths enter the Two way chromatograms post HW highway of the logical selector valve of two dimension ten
It is connected to fraction collector；
The one-dimensional chromatographic isolation state of sample is connected to pump into system input pipe with A mouths, Jing B mouths and sampling system input pipe
Connection, C mouths are connected with sampling system outlet tube, and D mouths are connected with detecting system input pipe, 5 chromatographic columns of clematis stem post selector valve
One of enter detecting system, Jing threeway blender, detecting system outlet tube enter G mouths after by the one-dimensional chromatographic column input pipes of H mouth Jing,
The 1 one-dimensional chromatograph enrichment post and one-dimensional chromatographic column outlet tube selected by one-dimensional ten logical selector valves enters F mouths, and Jing E mouths enter two
The Two way chromatograms post HW highway of the logical selector valve of dimension ten is connected to fraction collector；Meanwhile, diluting pump, threeway blender, inspection
Examining system outlet tube is connected to G mouths；
Second connection status is connected to pump into system input pipe with A mouths, and Jing H mouths connect with one-dimensional chromatographic column input pipe
Connect, then wherein 1 of the one-dimensional ten logical selector valve selection 18 one-dimensional chromatograph enrichment posts of series connection of Jing is used as loading post, the one-dimensional chromatographs of Jing
Post outlet tube enters E mouths, and after testing system input pipe is separated D mouths with after wherein 1 Coupled columns of clematis stem post selector valve, is entered
Enter detector, Jing threeway blender, detecting system outlet tube, into after G mouths by F mouth Jing Two way chromatograms post input pipes and by two dimension
Fraction collector is connected to after 1 two grades of enriching column series connection enrichment that ten logical selector valves are selected.
One-dimensional chromatograph enrichment post is selected and develops post identical filler with separation method, and described filler is positive silica gel, carries
The reverse phase silica gel matrix fill of C18, Xion, C8, cyano group or amino or macroporous adsorbent resin.
A kind of collection separation method exploitation, the work of the natural drug two dimension preparative chromatograph of ON-LINE SEPARATION-be enriched in one
Method, comprises the following steps：
1）Separation method is developed
The chromatograph is in the first connection status by two eight ways valves of control, injects a sample into two clematis stem sample introductions
Valve, opens the first pump and the second pump group into the liquid chromatographic system of gradient elution, and sample enters separation method and develops post, by detecting
Instrument gathers chromatogram, adjustment liquid chromatogram mobile phase ratio and elution program, and chromatograph performs method Optimizing Mode, makes sample reality
After now farthest separating；Gained separation condition is inserted in control system；Mobile phase waste liquid passes through No. H of two eight ways valves
After mouthful, flowed out by the HW highway of one-dimensional chromatographic column and Two way chromatograms post；
2）One-dimensional separation
The chromatograph is in the first connection status by two eight ways valves of control, injects a sample into two clematis stem sample introductions
Valve, six-way valve selects one-dimensional separation chromatography post, opens the first pump and the second pump group into the liquid chromatographic system of gradient elution, by examining
Instrument collection chromatogram is surveyed, chromatograph is performed one-dimensional clastotype, separated using the separation condition inserted in control system；
The selected diluent of diluting pump injection is opened, object is separated into into 18 fractions according to temporal sequence；Each fraction passes through two
After the H mouths of eight ways valve, in being enriched in 18 one-dimensional chromatographic columns, mobile phase waste liquid Jing Two way chromatograms posts HW highway flows out；
3）Two dimensional separation
The chromatograph is in the second connection status by two eight ways valves of control, separates object to be enriched in one-dimensional chromatograph
18 components in post, six-way valve selects two dimensional separation chromatographic column, open the first pump and the second pump group into can gradient elution liquid
Phase chromatographic system, by detector chromatogram is gathered, and chromatograph performs two dimensional separation pattern, using dividing for inserting in control system
Separated from condition, the selected diluent of diluting pump injection is opened, according to chromatographic peak collection mode by 18 enriching columns
Component be individually separated into monomeric compound；Each composition is enriched in respectively 9 Two way chromatograms after the F mouths of two eight ways valves
In post, mobile phase waste liquid flows out Jing after Two way chromatograms post；
4）Fraction is reclaimed
The removal process that the further isolated monomeric compound of 18 fractions and 18 fractions is obtained after one-dimensional separation is equal
Can independently or respectively carry out after the completion of separation.Using open diluting pump, input eluant as mobile phase, make target into
Decompose analysis to be dissolved in small size mobile phase, recovery sample is in fraction collector；
The diluent is water, saline solution, methanol, acetonitrile, acetone, ethanol or normal alkane solvent；The eluant is
The conventional organic solvent such as methanol, acetonitrile, ethanol, water and its mixture, n-alkane；
In the application, one-dimensional chromatographic column is selected and separation method exploitation identical filler used, is reduced separation preparation and is put
The difficulty of big experiment, may be selected silica gel, reverse phase silica gel matrix fill such as C18, Xion, C8, CN base, amino, macroporous adsorbent resin
Deng.Two way chromatograms select have the detached filler of good orthogonality with one-dimensional chromatograph.Enriching column selects to be filled out with detached dowel identical
Material.The diluent that enrichment process is selected is to be conducive to strengthening the solvent that sample retains in trapping column, including water, saline solution, first
Alcohol, acetonitrile, acetone, ethanol, normal alkane solvent or the solvent that other eluting powers are little and volatility is high.
The beneficial effect of instrument of the present invention is：Separation method exploitation and optimization, natural drug are realized on same instrument
Two-dimensional quadrature chromatographic isolation prepare.By complicated Separation of Natural Products into 18 repeatable components for obtaining or effective site,
Two dimensional separation can make 18 positions further isolated monomeric compound, whole mask work work under the control of the computer
Make.Being operated in several days of making that even several months script several weeks just can complete is completed, and greatly improves the natural drug such as Chinese medicine system
System property separates the efficiency for preparing, and provides for Chinese medicine, plant amedica, marine drug effective site, the preparative separation of composition, screening active ingredients
Efficient, reliable platform.
Description of the drawings
Fig. 1 be collect separation method exploitation, the of the natural drug two dimension preparative chromatograph of ON-LINE SEPARATION-be enriched in one
One connection status figure.
Fig. 2 is the connection figure of two eight ways valves in the connection shape body of chromatograph first.
Fig. 3 be collect separation method exploitation, the second of the natural drug two dimension preparative chromatograph of ON-LINE SEPARATION-be enriched in one
Connection status figure.
Fig. 4 is the connection figure of two eight ways valves in the connection shape body of chromatograph second.
In figure：1st, the first pump, the 2, second pump, 3, diluting pump, 4, blender, 4a, system input pipe is pumped into, 5, two six
Logical injection valve, 5a, sampling system input pipe, 5b, sampling system outlet tube, 6, clematis stem post selector valve, 6a, detecting system input
Pipe, 6b, chromatographic column, 6c, clematis stem post selector valve arm, 7, detector, 8, threeway blender, 8a, detecting system outlet tube, 9,
One-dimensional chromatograph enrichment post, 9a, one-dimensional chromatographic column input pipe, 9b, one-dimensional chromatographic column outlet tube, 9c, one-dimensional chromatographic column HW highway,
9d, one-dimensional ten through posts selector valve, 10, Two way chromatograms post, 10a, Two way chromatograms post input pipe, 10b, Two way chromatograms post are public logical
Road, 10c, two-dimentional ten through posts selector valve, 11, fraction collector, 12, two eight ways valves.
Specific embodiment
Embodiment：The systemic Two way chromatograms of glycyrrhiza uralensis fisch water-soluble substanceses are separated and prepared
The present embodiment is only limitted to be described in further details the present invention, rather than limitation of the invention.
The separation of highly polar compound is the detached difficult point of chemical composition of Chinese materia medica, in view of anti-phase C18 chromatographic applications is extensive
Property and hydrophilic chromatographic be often used as supplementing for reversed phase chromatography and be used for the separation of highly polar compound, using both chromatographic isolation
Orthogonality, to the water soluble ingredient of glycyrrhiza uralensis fisch systematicness separation preparation is carried out..
The present embodiment is using XIon/C18 two dimension ON-LINE SEPARATION modal cutoff Radix Glycyrrhizae aqueous samples, and detailed process is as follows：
1）Separation method is developed
1.1 two eight ways valves are in first state, Radix Glycyrrhizae aqueous samples methanol solution 5mg/ml 500ul are noted every time
Enter clematis stem injector, six-way valve system of selection exploitation chromatographic column XION (10mm × 150mm, 10 μm).Opening pump A, B composition can
The liquid chromatographic system of gradient elution, with methanol/water（95/100—5/100）Separated for mobile phase, chromatographic work station is held
Row method development mode, in all-wave length UV230II UV-detector chromatogram is gathered, and according to detached situation, adjusts liquid phase color
Spectrum mobile phase ratio and elution program, after making sample realize farthest separating, determine that separation condition is：A is water, and B is first
Alcohol, gradient elution：0-13 min, 95%～85%B；13～33 min, 85%～60%B；33～80 min, 60%～5%
B；Flow velocity is 8 mL/min；Ultraviolet detection wavelength is 254 nm.Separation condition will be obtained to insert in one-dimensional preparation procedure.
1.2 using selected mobile phase condition, according to the one-dimensional detached dowel of 1.1 process selecting be XION (20mm ×
250mm, 10 μm) separation preparation is carried out, detached mobile phase is led to after the H mouths of two eight ways valves by 3 the public of ten-way valve
Road exits into fraction collection instrument, and 1 fraction was collected per 15 minutes, and 18 fractions are collected altogether.
1.3 select anti-phase C18 separation methods exploitation chromatographic column (10mm × 150mm, 10 μm), ultraviolet detection wavelength
254nm, chromatographic condition：Mobile phase：A is water, and B is methanol, gradient elution：0～60min, carries out chromatograph point between 5%～95%B
From methods experiment, sample size is 100 μ L, and the fraction obtained to 1.2 carries out separation method selection and optimization.Each method of optimization
Insert in two dimensional separation method software.
1.4 two eight ways valves are in first state, six-way valve select one-dimensional chromatography column XIon (250mm × 20mm,
10 μm), call in one-dimensional separable programming i.e. high-pressure pump：A is water, and B is methanol, gradient elution：0-13 min, 95%～85%B；13
～33 min, 85%～60%B；33～80 min, 60%～5%B；Flow velocity is 10 mL/min；Ultraviolet detection wavelength is 254
Nm, one-dimensional enriching column is 18 XIon (20mm × 80mm, 10 μm), and Radix Glycyrrhizae water solublity sample injection volume is 50mg/ml, 4ml.
The solvent of diluting pump C is methanol, and sample, every one-dimensional enriching column Vavle switchings of 15min 1 time, is divided into 18 and is evaporated by 10ml/min
Point, it is enriched on 18 one-dimensional enriching columns.The HW highway of mobile phase waste liquid Jing two dimension enriching column ten-way valves flows out.
The component of 1.5 pairs of 18 fractions is respectively adopted C18 posts and carries out two dimensional separation.
Two eight ways valves are placed in into the second state, using 18 components in one-dimensional enriching column as sample, are opened using method
Send out in obtain two-dimensional columns separation condition, successively to being enriched in one-dimensional enriching column in sample separate, with peak collection mode
Each monomeric compound is enriched in two-dimentional enriching column.Two way chromatograms detached dowel C18 (20mm × 250mm, 10 μm), ultraviolet inspection
Survey wavelength 254nm, two-dimentional enriching column be C18 (20mm × 80mm, 10 μm), chromatographic condition：Mobile phase：A is water, and B is methanol, is washed
De- program is according to the 1.3 each fraction conditions for determining.The solvent of diluting pump C be water, 10ml/min.
1.6 samples are reclaimed：After each component of 18 fractions completes to separate, pump C is opened, use 200-300ml methanol solutions
By two dimension enrichment ten-way valve, eluting is enriched in the monomeric compound on two-dimentional enriching column in fraction collection instrument, after concentration
The purity and structure of authenticating compound.
After separation, Jing after TLC and HPLC is detected simultaneously concentration is merged, 32 components that 4.9mg to 55mg is not waited is obtained,
Wherein more than 90% monomeric compound of content 29, the simple component of two compounds 3.
Claims (2)
1. the natural drug two dimension preparative chromatograph of a kind of collection separation method exploitation, ON-LINE SEPARATION-be enriched in one, it includes control
System processed, it is characterised in that：It includes pumping into system, sampling system, detecting system, one-dimensional chromatogram column system, Two way chromatograms post
System and two eight ways valves（12）, two eight ways valves（12）Including A mouths, B mouths, C mouths, D mouths, E mouths, F mouths, G mouths and H mouths；
The system that pumps into is including the first pump（1）, the second pump（2）And blender（4）, blender（4）It is connected with and pumps into system input pipe
（4a）；The sampling system includes sampling system input pipe（5a）, two six-way injection valves（5）, sampling system outlet tube（5b）；
The detecting system includes the detecting system input pipe for setting gradually（6a）, clematis stem post selector valve（6）Clematis stem post selector valve
Pipe（6c）, detector（7）, threeway blender（8）With detecting system outlet tube（8a）；The one-dimensional chromatographic column piece-rate system is adopted
It is connected to clematis stem post selector valve（6）5 chromatographic columns（6b）One of as detached dowel, be again sequentially connected one-dimensional chromatographic column input pipe
（9a）, two series connection one-dimensional ten through posts selector valve（9d）, 18 one-dimensional chromatograph enrichment posts（9）With one-dimensional chromatographic column outlet tube
（9b）；The Two way chromatograms column system adopts 18 one-dimensional chromatograph enrichment posts（9）One of as loading post, be connected to clematis stem post choosing
Select valve（6）5 chromatographic columns（6b）One of as detached dowel, be again sequentially connected Two way chromatograms post input pipe（10a）, two dimension ten lead to
Post selector valve（10c）And 9 Two way chromatograms enriching columns（10）And fraction collector（11）；Diluting pump（3）It is connected to threeway to mix
Clutch（8）；Control system and the first pump（1）, the second pump（2）, diluting pump（3）, two eight ways valves（12）, two clematis stem sample introductions
Valve（5）, clematis stem post selector valve（6）, detector（7）One-dimensional ten through posts selector valve（9d）, two-dimentional ten through posts selector valve（10c）Carry out
Electrical connection, control system controls the first pump（1）, the second pump（2）And diluting pump（3）Unlatching and stopping, control system control
Two eight ways valves（12）, two six-way injection valves（5）, clematis stem post selector valve（6）, one-dimensional ten through posts selector valve（9d）, two dimension ten
Through post selector valve（10c）Break-make switching；
The chromatograph is by controlling two eight ways valves（12）Connection in the first connection status or the second connection status；
First connection status is divided into the one-dimensional chromatographic isolation state of sample separation method development status and sample；Sample is separated
Method development status are to pump into system input pipe（4a）It is connected with A mouths, Jing B mouths and sampling system input pipe（5a）Connection, C mouths with
Sampling system outlet tube（5b）Connection, D mouths and detecting system input pipe（6a）Connection, clematis stem post selector valve（6）Clematis stem post choosing
Select valve arm（6c）Into detecting system, Jing threeway blenders（8）, detecting system outlet tube（8a）, into after G mouths by H mouth Jing
One-dimensional chromatographic column input pipe（9a）, one-dimensional chromatographic column HW highway（9c）With one-dimensional chromatographic column outlet tube（9b）Into F mouths, Jing E
Mouth enters the logical selector valves of two dimension ten（10c）Two way chromatograms post HW highway（10b）It is connected to fraction collector（11）；
The one-dimensional chromatographic isolation state of sample is to pump into system input pipe（4a）It is connected with A mouths, Jing B mouths and sampling system input pipe
（5a）Connection, C mouths and sampling system outlet tube（5b）Connection, D mouths and detecting system input pipe（6a）Connection, clematis stem post selector valve
（6）5 chromatographic columns（6b）One of enter detecting system, Jing threeway blenders（8）, detecting system outlet tube（8a）Into G mouths
Afterwards by the one-dimensional chromatographic column input pipes of H mouth Jing（9a）, by one-dimensional ten logical selector valves（9d）The 1 one-dimensional chromatograph enrichment post for selecting（9）
With one-dimensional chromatographic column outlet tube（9b）Into F mouths, Jing E mouths enter the logical selector valve of two dimension ten（10c）Two way chromatograms post it is public logical
Road（10b）It is connected to fraction collector（11）；Meanwhile, diluting pump（3）, threeway blender（8）, detecting system outlet tube（8a）
It is connected to G mouths；
Second connection status is to pump into system input pipe（4a）It is connected with A mouths, Jing H mouths and one-dimensional chromatographic column input pipe（9a）
Connection, then the one-dimensional ten logical selector valves of Jing（9d）Select 18 one-dimensional chromatograph enrichment posts of series connection（9）Wherein 1 as loading post,
The one-dimensional chromatographic column outlet tubes of Jing（9b）Into E mouths, D mouths system input pipe after testing（6a）With clematis stem post selector valve（6）Wherein 1
Individual chromatographic column（6b）Separate after series connection, into detector（7）, Jing threeway blenders（8）, detecting system outlet tube（8a）, enter G
By F mouth Jing Two way chromatograms post input pipes after mouthful（10a）Lead to selector valve with by two dimension ten（10c）The 1 two grades of enriching column for selecting
（10）Fraction collector is connected to after series connection enrichment（11）.
2. a kind of collection separation method exploitation according to claim 1, the natural drug two dimension of ON-LINE SEPARATION-be enriched in one
Preparative chromatograph, it is characterised in that：One-dimensional chromatograph enrichment post is selected and develops post identical filler with separation method, and described filler is
Positive silica gel, the reverse phase silica gel matrix fill with C18, Xion, C8, cyano group or amino or macroporous adsorbent resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620666461.7U CN206132715U (en) | 2016-06-30 | 2016-06-30 | Development of collection separation method , online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620666461.7U CN206132715U (en) | 2016-06-30 | 2016-06-30 | Development of collection separation method , online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206132715U true CN206132715U (en) | 2017-04-26 |
Family
ID=58561099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620666461.7U Withdrawn - After Issue CN206132715U (en) | 2016-06-30 | 2016-06-30 | Development of collection separation method , online separation enrichment is in crude drug of an organic whole two dimension preparative chromatograph |
Country Status (1)
| Country | Link |
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| CN (1) | CN206132715U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105938130A (en) * | 2016-06-30 | 2016-09-14 | 朱靖博 | Natural drug two-dimensional preparation chromatographic instrument with integrated functions of separation method development and online separation-enrichment and work method thereof |
| CN107607661A (en) * | 2017-09-05 | 2018-01-19 | 湖南师范大学 | Multi-dimensional chromatograph system |
| CN109541051A (en) * | 2018-11-12 | 2019-03-29 | 复旦大学 | The online concentrated solvent switch of two-dimensional liquid chromatography interface |
-
2016
- 2016-06-30 CN CN201620666461.7U patent/CN206132715U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105938130A (en) * | 2016-06-30 | 2016-09-14 | 朱靖博 | Natural drug two-dimensional preparation chromatographic instrument with integrated functions of separation method development and online separation-enrichment and work method thereof |
| CN107607661A (en) * | 2017-09-05 | 2018-01-19 | 湖南师范大学 | Multi-dimensional chromatograph system |
| CN109541051A (en) * | 2018-11-12 | 2019-03-29 | 复旦大学 | The online concentrated solvent switch of two-dimensional liquid chromatography interface |
| CN109541051B (en) * | 2018-11-12 | 2021-08-17 | 复旦大学 | Two-dimensional liquid chromatography interface on-line concentration solvent exchange device |
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