CN107607661A - Multi-dimensional chromatograph system - Google Patents
Multi-dimensional chromatograph system Download PDFInfo
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- CN107607661A CN107607661A CN201710792504.5A CN201710792504A CN107607661A CN 107607661 A CN107607661 A CN 107607661A CN 201710792504 A CN201710792504 A CN 201710792504A CN 107607661 A CN107607661 A CN 107607661A
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Abstract
A kind of multi-dimensional chromatograph system, including collector, supplement pipeline and multiple chromatographic columns；Wherein at least two chromatographic column is respectively the first chromatographic column and the second chromatographic column, and the first chromatographic column is used to be connected to be separated with the first flowing；The sample that collector is used to collecting after the first chromatogram post separation in the second chromatographic column to be separated；When second chromatographic column is separated, one end of collector is used to be connected with the second flowing, and the other end is connected with the second chromatographic column, and the sample after the first chromatogram post separation is transferred into the second chromatographic column is separated；One end of supplement pipeline is used to be connected with the second flowing, one end that the other end is communicated in the second chromatographic column with collector connects, converge sample of the second mobile phase in supplement pipeline with being come out from collector before the second chromatogram post separation, therefore content of first mobile phase in the sample into the second chromatogram post separation is treated is reduced, so as to inhibit chromatogram peak stretching, so as to improve chromatographic isolation effect.
Description
Technical field
The present invention relates to technical field of chromatography separation, more particularly to a kind of multi-dimensional chromatograph system.
Background technology
With the fast development of chromatographic separation technology, the clastotype of chromatogram is also gradually sent out from one-dimensional mode to multi-dimensional model
Exhibition.Multidimensional chromatography refers to that sample passes through the separation method of two or more chromatogram modes, compared to one-dimensional mode, its significant increase
Separating capacity.The mobile phase used yet with the chromatogram mode of two kinds of different mechanism is typically incomplete same, therefore through upper
After one-dimensional chromatographic isolation, it is transferred to down the mobile phase that bidimensional chromatographic isolation when one-dimensional chromatogram further separates be present and incompatible asks
Topic, so as to cause the serious broadening of chromatographic peak, and then make it that separating effect is bad.
The content of the invention
Based on this, it is necessary to provide a kind of separative efficiency high multi-dimensional chromatograph system.
A kind of multi-dimensional chromatograph system, including collector, supplement pipeline and multiple chromatographic columns；Wherein at least two chromatographic column
Respectively the first chromatographic column and the second chromatographic column, first chromatographic column are used to be connected to be separated with the first flowing, institute
Collector is stated for the sample after collecting the first chromatogram post separation to be separated in second chromatographic column；Described second
When chromatographic column is separated, one end of the collector is used to be connected with the second flowing, the other end and second chromatographic column
Connection, is transferred to second chromatographic column by the sample after the first chromatogram post separation and is separated；The supplement pipeline
One end is used to be connected with the described second flowing, and one end that the other end is communicated in second chromatographic column with the collector connects
It is logical.
Above-mentioned multi-dimensional chromatograph system, sample to be separated are first separated in the first chromatographic column using the first mobile phase, are collected
Device collects the sample after the first chromatogram post separation.Sample after first chromatogram post separation is transferred to the second chromatographic column from collector and carried out
When separating again, the sample in collector is pushed into by the second chromatographic column using second mobile phase different from the first mobile phase, by
Sample in collector carries the first mobile phase, therefore it is incompatible to have two kinds of mobile phases when being transferred to the second chromatogram post separation
The problem of causing chromatogram peak stretching.And use supplement pipeline to supplement the second mobile phase, and make the second mobile phase of the supplement the
Converge before two chromatogram post separations with the sample come out from collector, reduce the first mobile phase and waiting to enter the second chromatographic column point
From sample in content so that the component in sample in the second chromatogram post separation can well column cap be enriched with, suppression
Chromatogram peak stretching is made, so as to improve chromatographic isolation effect.
In one of the embodiments, the supplement pipeline is provided with flow control assembly.
In one of the embodiments, the collector is quantitative loop or trapping column.
In one of the embodiments, first chromatographic column is reverse-phase chromatographic column, and second chromatographic column is hydrophilic color
Compose post.
In one of the embodiments, two collections are provided between first chromatographic column and second chromatographic column
Device, one of them is separated for the sample after the first chromatogram post separation to be transferred into second chromatographic column, another
For continuing to collect the sample after the first chromatogram post separation, and two collectors alternately collect first chromatogram
Sample after post separation is simultaneously transferred to second chromatographic column to be separated.
In one of the embodiments, in addition to switching valve, first chromatographic column, second chromatographic column and described
Two collectors between one chromatographic column and second chromatographic column connect with the switching valve respectively, the switching valve energy
Enough switching states, so that two collectors alternately collect the sample after the first chromatogram post separation and are transferred to institute
The second chromatographic column is stated to be separated.
In one of the embodiments, the switching valve is two eight ways valves, two ten-way valves or connected by two six-way valves
Connect composition.
In one of the embodiments, when the switching valve is two eight ways valves, the switching valve has eight ports, according to
Secondary is first port, second port, the 3rd port, the 4th port, fifth port, the 6th port, the 7th port and the 8th port；
First chromatographic column connects with the first port, and second chromatographic column connects with the 7th port；Its
In the both ends of a collector connected respectively with the second port and the 6th port, another collector
Both ends connect with the 4th port and the 8th port respectively；The fifth port is used to discharge waste liquid；
The switching valve has two states and can switched between described two states；One of state is：Institute
State first port to connect with the second port, the 3rd port connects with the 4th port, the fifth port and institute
The connection of the 6th port is stated, the 7th port connects with the 8th port；Another state is：The first port and described the
Eight ports connect, and the second port connects with the 3rd port, and the 4th port connects with the fifth port, described
6th port connects with the 7th port.
In one of the embodiments, when the switching valve is two ten-way valves, the switching valve has ten ports, according to
It is secondary for first port, second port, the 3rd port, the 4th port, fifth port, the 6th port, the 7th port, the 8th port,
9th port and the tenth port；
First chromatographic column connects with the first port, and second chromatographic column connects with the 9th port；Its
In the both ends of a collector connected respectively with the 3rd port and the tenth port, another collector
Both ends connect with the fifth port and the 8th port respectively；6th port is used to discharge waste liquid；
The switching valve has two states and can switched between described two states；One of state is：Institute
State first port to connect with the tenth port, the second port connects with the 3rd port, the 4th port and institute
Fifth port connection is stated, the 6th port connects with the 7th port, and the 8th port connects with the 9th port；
Another state is：The first port connects with the second port, and the 3rd port connects with the 4th port, described
Fifth port connects with the 6th port, and the 7th port connects with the 8th port, the 9th port with it is described
Tenth port connects.
In one of the embodiments, when the switching valve is made up of two six-way valves, one of six-way valve has six
Individual port, it is followed successively by first port, second port, the 3rd port, the 4th port, fifth port and the 6th port；Another six
Port valve has six ports, is followed successively by the 7th port, the 8th port, the 9th port, the tenth port, the tenth Single port and the 12nd
Port；
The second port connects with the 7th port, and the 4th port connects with the tenth Single port；It is described
First chromatographic column connects with the first port；Second chromatographic column connects with the fifth port；One of them described receipts
The both ends of storage connect with the 3rd port and the 6th port respectively, the both ends of another collector respectively with institute
The 9th port is stated to connect with the tenth Two-port netwerk；
The switching valve has two states and can switched between described two states；One of state is：Institute
State first port to connect with the 6th port, the second port connects with the 3rd port, the 4th port and institute
Fifth port connection is stated, the 7th port connects with the 8th port, and the 9th port connects with the tenth port,
Tenth Single port connects with the tenth Two-port netwerk；Another state is：The first port connects with the second port,
3rd port connects with the 4th port, and the fifth port connects with the 6th port, the 7th port with
The tenth Two-port netwerk connection, the 8th port connect with the 9th port, the tenth port and described tenth one end
Mouth connection.
Brief description of the drawings
Fig. 1 is the structure chart of two working conditions of the multi-dimensional chromatograph system of an embodiment；
Fig. 2 is the structure chart of two working conditions of the multi-dimensional chromatograph system of another embodiment；
Fig. 3 is the structure chart of a working condition of the multi-dimensional chromatograph system of another embodiment；
Fig. 4 is the structure chart of the another working condition of multi-dimensional chromatograph system shown in Fig. 3；
Fig. 5 is conventional multidimensional chromatographic system and the multi-dimensional chromatograph system shown in Fig. 1 be applied to it is used first-class during separation
The dynamic mutually gradient figure with the second mobile phase；
Fig. 6 is that conventional multidimensional chromatographic system is applied to separation ginsenoside root extract, obtained full two-dimension analysis spectrogram；
Fig. 7 is that multi-dimensional chromatograph system shown in Fig. 1 is applied to separation ginsenoside root extract, obtained full two-dimension analysis spectrum
Figure.
Embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But the present invention can realize in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention
The implication that technical staff is generally understood that is identical.Term used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
The arbitrary and all combination of the Listed Items of pass.
Referring to Fig. 1, the multi-dimensional chromatograph system 10 of an embodiment of the present invention include collector 100, supplement pipeline 200 and
Multiple chromatographic columns.
Wherein at least two chromatographic column is respectively the first chromatographic column 300 and the second chromatographic column 400, the first chromatographic column 300
For being connected with the first mobile phase 20 to be separated.
Collector 100 is used to collect the sample after the first chromatographic column 300 separates to be separated in the second chromatographic column 400；
When second chromatographic column 400 is separated, one end of collector 100 is used to connect with the second mobile phase 30, the other end and the second color
Spectrum post 400 connects, and the sample after the first chromatographic column 300 is separated is transferred to the second chromatographic column 400 and separated.
One end of supplement pipeline 200 is used to connect with the second mobile phase 30, and the other end is communicated in the second color with collector 100
Compose post 400 one end connection so that supplement pipeline 200 in the second mobile phase 30 the second chromatographic column 400 separation before with from
The sample that collector 100 comes out converges.
Above-mentioned multi-dimensional chromatograph system 10, sample to be separated are first divided in the first chromatographic column 300 using the first mobile phase 20
From collector 100 collects the sample after the separation of the first chromatographic column 300.Sample after the separation of first chromatographic column 300 is from collector
100 are transferred to the second chromatographic column 400 when being separated again, will be collected using second mobile phase 30 different from the first mobile phase 20
Sample in device 100 is pushed into the second chromatographic column 400, because the sample in collector 100 carries the first mobile phase 20, therefore is transferred to
Second chromatographic column 400 can have two kinds of mobile phases incompatible the problem of causing chromatogram peak stretching when separating.And use supplement pipeline
200 the second mobile phases 30 of supplement, and make the second mobile phase 30 of the supplement before the separation of the second chromatographic column 400 with from collector
100 sample out converges, and reduces the first mobile phase 20 and is waiting to enter the content in the sample of the second chromatographic column 400 separation,
So that the component in sample can be enriched with column cap well when the second chromatographic column 400 separates, it is suppressed that chromatogram peak stretching,
So as to improve chromatographic isolation effect.
Specifically, in addition to the first chromatogram pump and the second chromatogram pump.First chromatographic column 300 is connected with the first chromatogram pump, with
For the first mobile phase to be injected into the first chromatographic column 300.Second chromatogram pump is connected with collector 100, for second is flowed
Mutually injection collector 100, reinjects the second chromatographic column 400.
In one of the embodiments, supplement pipeline 200 is provided with flow control assembly 210.Flow control assembly 210 is used
The flow of the second mobile phase 30 in pipeline 200 is supplemented in control, is waiting to enter the second color to better control over the first mobile phase 20
The content in the sample that post 400 separates is composed, so as to reach more preferable separating effect.
Further, flow control assembly 210 can be flow valve or quantitative loop.
In one of the embodiments, the first chromatographic column 300 is reverse-phase chromatographic column, and the second chromatographic column 400 is hydrophilic chromatographic
Post.
Such first chromatographic column 300 separates compound in sample to be separated according to the descending order of polarity successively, washes
De- composition enters collector 100.The component that second chromatographic column 400 shifts after first chromatographic column 300 is separated according to polarity by
It is small to carry out second of separation to big order.Usually, now the first mobile phase 20 used is rich water mobile phase, and the second flowing
Phase 30 is high organic phase mobile phase, therefore higher, the more moisture of moisture from the sample after the separation of the first chromatographic column 300
Into the second chromatographic column 400, the retention of the second chromatographic column 400 can be reduced, causes the broadening of chromatographic peak.And use supplement pipe
Road 200 supplements the second mobile phase 30, and the moisture is little by little diluted, progresses into the second chromatographic column 400, avoids the moisture from passing through
The problem of irreversible chromatogram peak stretching is disposably caused from the push-in of collector 100 by the second mobile phase 30, so as to inhibit
Chromatogram peak stretching, improve chromatographic isolation effect.
In one of the embodiments, two collectors 100 are provided between the first chromatographic column 300 and the second chromatographic column 400.
One of them is used for the sample after the first chromatographic column 300 is separated and is transferred to the second chromatographic column 400 and is separated, another be used for after
The continuous sample collected after the separation of the first chromatographic column 300, and after two collectors 100 alternately collect the separation of the first chromatographic column 300
Sample and be transferred to the second chromatographic column 400 to be separated.
Such first chromatographic column 300 is constantly separated under the elution of the first mobile phase 20, and two collectors 100 are handed over
The sample separated in real time alternately and is by stages transferred to the second chromatographic column 400；Collect and terminate when one of collector 100
Afterwards, switch to another collector 100 to continue to collect the sample of the first chromatographic column 300 separation, while the collection there are the receipts of sample
Storage 100 is connected with the second mobile phase 30 and the second chromatographic column 400, and sample therein is transferred into the second chromatographic column 400 is carried out
Separation.
Especially, the first chromatographic column 300 and the second chromatographic column are passed through in the separation analysis of all the components in sample to be separated
400 separation, all the components are divided with equal proportion by the first chromatographic column 300 and the second chromatographic column 400 in sample to be separated
The problem of being detected from after, therefore avoiding the poor reproducibility of the loss of sample and sample in separation process.
Further, collector 100 is quantitative loop or trapping column.Further, flow control assembly 210 and collector
100 when being quantitative loop, preferably capacity identical quantitative loop.
Specifically in the present embodiment, collector 100 is anti-phase trapping column.In one of the embodiments, when collector 100
For anti-phase trapping column when, in addition to dilution pipeline 310.Dilution one end of pipeline 310 connects with retarder thinner 50, the other end and first
The port of export connection of chromatographic column 300, so that retarder thinner 50 converges with the sample separated by the first chromatographic column 300.Usually,
First mobile phase 20 used is rich water mobile phase, and retarder thinner 50 is high aqueous phase, and the sample separated by the first chromatographic column 300 enters
One step passes through the dilution of retarder thinner 50 so that and the water content treated into the sample of anti-phase trapping column improves, and organic phase reduces, with
The target analytes in sample are made preferably to be inverted trapping column supplementary set.
When collector 100 is quantitative loop, the dilution pipeline 310 can omit.It is appreciated that in Fig. 2~Fig. 4, work as receipts
When storage 100 is anti-phase trapping column, dilution pipeline 310 (not showing in Fig. 2~Fig. 4) can be also provided with.
In one of the embodiments, in addition to detection means 500, the outlet of the chromatographic column 400 of detection means 500 and second
End connection.Specifically, detection means 500 is the analysis and detection devices 500 such as chromatograph.
Refer to Fig. 1, Fig. 2, Fig. 3 and Fig. 4, in one of embodiment, in addition to switching valve 600/700/800.First
Two collectors 100 between chromatographic column 300, the second chromatographic column 400 and the first chromatographic column 300 and the second chromatographic column 400 are distinguished
600/700/800 is connected with switching valve.Switching valve 600/700/800 allows hand over state, so that two collectors 100 are alternately
Collect the sample after the separation of the first chromatographic column 300 and be transferred to the second chromatographic column 400 to be separated.
Further, switching valve 600/700/800 is for two eight ways valves, two ten-way valves or by two six-way valve connection groups
Into.
Referring to Fig. 1, further, in one embodiment, switching valve 600 is two eight ways valves.Switching valve 600 has eight
Individual port, it is followed successively by first port 610, second port 620, the 3rd port 630, the 4th port 640, fifth port the 650, the 6th
Port 660, the 7th port 670 and the 8th port 680.Wherein the first chromatographic column 300 connects with first port 610.Second chromatogram
Post 400 connects with the 7th port 670.The both ends of one of collector 100 respectively with the port 660 of second port 620 and the 6th
Connection, the both ends of another collector 100 connect with the 4th port 640 and the 8th port 680 respectively.Fifth port 650 is used for
Discharge waste liquid 40.
Switching valve 600 has two states and can switched between two states.One of state is：First end
Mouth 610 connects with second port 620, and the 3rd port 630 connects with the 4th port 640, the port 660 of fifth port 650 and the 6th
Connection, the 7th port 670 connects with the 8th port 680.Another state is：First port 610 connects with the 8th port 680, the
Two-port netwerk 620 connects with the 3rd port 630, and the 4th port 640 connects with fifth port 650, the 6th port 660 and the 7th port
670 connections.
During work, switching valve 600 alternately switches in two states.Specifically, the waste liquid 40 and the 5th of detection means 500
The waste liquid 40 that port 650 is discharged converges discharge system.
Referring to Fig. 2, further, in another embodiment, switching valve 700 is two ten-way valves.Switching valve has ten
Individual port, it is followed successively by first port 701, second port 702, the 3rd port 703, the 4th port 704, fifth port the 705, the 6th
Port 706, the 7th port 707, the 8th port 708, the 9th port 709 and the tenth port 710.Wherein the first chromatographic column 300 with
First port 701 connects.Second chromatographic column 400 connects with the 9th port 709.The both ends of one of collector 100 respectively with
3rd port 703 connects with the tenth port 710, the both ends of another collector 100 respectively with the port of fifth port 705 and the 8th
708 connections.6th port 706 is used to discharge waste liquid 40.
Switching valve 700 has two states and can switched between two states.One of state is：First end
Mouth 701 connects with the tenth port 710, and second port 702 connects with the 3rd port 703, the 4th port 704 and fifth port 705
Connection, the 6th port 706 connect with the 7th port 707, and the 8th port 708 connects with the 9th port 709.Another state is：The
Single port 701 connects with second port 702, and the 3rd port 703 connects with the 4th port 704, the port of fifth port 705 and the 6th
706 connections, the 7th port 707 connect with the 8th port 708, and the 9th port 709 connects with the tenth port 710.
During work, switching valve 700 alternately switches in two states.Specifically, the waste liquid 40 and the 6th of detection means 500
The waste liquid 40 that port 706 is discharged converges discharge system.
Fig. 3 and Fig. 4 is referred to, further, in another embodiment, switching valve 800 is made up of two six-way valve connections.
One of six-way valve 810 has six ports, is followed successively by first port 801, second port 802, the 3rd port the 803, the 4th
Port 804, the port 806 of fifth port 805 and the 6th.Another six-way valve 820 has six ports, is followed successively by the 7th port
807th, the 8th port 808, the 9th port 809, the tenth port 810, the tenth Single port 811 and the tenth Two-port netwerk 812.Second port
802 connect with the 7th port 807, and the 4th port 804 connects with the tenth Single port 811.Wherein, the first chromatographic column 300 and first
Port 801 connects.Second chromatographic column 400 connects with fifth port 805.The both ends of one of collector 100 are respectively with the 3rd
Port 803 connects with the 6th port 806.The both ends of another collector 100 respectively with the 9th port 809 and the tenth Two-port netwerk
812 connections.
Switching valve 800 has two states and can switched between two states.
Referring to Fig. 3, one of state is：First port 801 connects with the 6th port 806, second port 802 and
Three ports 803 connect, and the 4th port 804 connects with fifth port 805, and the 7th port 807 connects with the 8th port 808, and the 9th
Port 809 connects with the tenth port 810, and the tenth Single port 811 connects with the tenth Two-port netwerk 812.
Referring to Fig. 4, another state is：First port 801 connects with second port 802, the 3rd port 803 and the 4th end
Mouth 804 connects, and fifth port 805 connects with the 6th port 806, and the 7th port 807 connects with the tenth Two-port netwerk 812, the 8th end
Mouth 808 connects with the 9th port 809, and the tenth port 810 connects with the tenth Single port 811.During work, switching valve is in two states
It is middle alternately to switch.
Specific in the present embodiment, chromatographic column is two.Now, the multi-dimensional chromatograph system 10 is Comprehensive two-dimensional LC.
It is understood that when the quantity of chromatographic column is three or more than three, the 3rd, the 4th chromatographic column etc. and previous chromatographic column it
Between connected also by collector, can also be provided with above-mentioned supplement pipeline.Preferably, two chromatographic columns adjacent on separation sequence are equal
Connected by collector, supplement pipeline with above-mentioned switching valve.
It is concrete application embodiment below.
Multi-dimensional chromatograph system and the present invention of the tradition without supplement pipeline multi-dimensional chromatograph system as shown in Figure 1 is respectively adopted
It is sequentially connected anti-phase and hydrophilic chromatographic and builds full two-dimentional system.Wherein, the first chromatographic column is reverse-phase chromatographic column：C18 chromatographic columns (20
× 4.6mm, 5 μm), flow velocity 1ml/min；Second chromatographic column is hydrophilic chromatographic post：HILIC chromatographic columns (25 × 4.6mm, 5 μm),
Flow velocity 1.5mL/min, the first collector are reversed-phase column (2 × 4.6mm, 5 μm), and the second collector is reversed-phase column (2 × 4.6mm, 5 μ
M), supplement connection reversed-phase column ((2 × 4.6mm, 5 μm)) on pipeline and be used as flow control assembly.
Ginsenoside root extract is used to analyze object, full two dimension point is carried out to Ginsenosides compound therein
Analysis.Sample preparation：Precision weighs ginseng extract 0.3g ultrasonic dissolutions in 5ml ethanol, after taking supernatant to cross 0.4 μm of organic film
Wait sample introduction.
First mobile phase and the second mobile phase are gradient elution.First mobile phase is the aqueous solution of acetonitrile, its acetonitrile
Volumetric concentration is 2%~50%.Second mobile phase is initially 100% acetonitrile, and then acetonitrile volumetric concentration gradually reduces.It is first-class
It is dynamic mutually as shown in Figure 5 with the concentration gradient of the second mobile phase.Abscissa is the time in Fig. 5, and ordinate is acetonitrile volumetric concentration, 1st
Dimensional continuous gradient and 2stDimensional continuous gradient are respectively first
Mobile phase gradient and the second mobile phase gradient.
Use the multi-dimensional chromatograph system of traditional multi-dimensional chromatograph system and the present invention in Detection wavelength for 203nm, enter respectively
Pedestrian joins the full two-dimension analysis of root extract, obtains full two-dimension analysis spectrogram, respectively as shown in Figure 6 and Figure 7.Horizontal stroke in Fig. 6 and Fig. 7
Coordinate is retention time, and ordinate is peak intensity.By the ginsenoside Rg1 peak base in Fig. 6 and Fig. 7 is wide, ginsenoside Re peak base
The information record such as width, Observable peak number mesh, system peak capacity is as shown in table 1.
Table 1
As shown in Table 1, by taking saponin(e Rg1 and Re in ginseng as an example, the more traditional multi-dimensional chromatograph of multi-dimensional chromatograph system of the present invention
System, there is advantages below：The color that a width of conventional multidimensional chromatographic system in the peak base of saponin(e Rg1 and Re chromatographic peak obtains in ginseng
The peak base of spectral peak it is wide 1/3, it is seen that reduce chromatographic peak width.Spectrogram Observable peak number mesh adds 20%, and system peak capacity carries
It is high by 50%.Because sample introduction concentration is certain, therefore the sensitivity of detection is greatly improved, significantly increase post effect and separation effect
Rate.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of multi-dimensional chromatograph system, it is characterised in that including collector, supplement pipeline and multiple chromatographic columns；Wherein at least have
Two chromatographic columns are respectively the first chromatographic column and the second chromatographic column, and first chromatographic column is used to be connected to enter with the first flowing
Row separation, the collector are used to collect the sample after the first chromatogram post separation in second chromatographic column to be divided
From；When second chromatographic column is separated, one end of the collector is used to be connected with the second flowing, the other end with it is described
Second chromatographic column is connected, and the sample after the first chromatogram post separation is transferred into second chromatographic column and separated；It is described
One end of supplement pipeline is used to be connected with the described second flowing, and the other end is communicated in second chromatographic column with the collector
One end connection.
2. multi-dimensional chromatograph system as claimed in claim 1, it is characterised in that the supplement pipeline is provided with flow control group
Part.
3. multi-dimensional chromatograph system as claimed in claim 1, it is characterised in that the collector is quantitative loop or trapping column.
4. multi-dimensional chromatograph system as claimed in claim 1, it is characterised in that first chromatographic column is reverse-phase chromatographic column, institute
It is hydrophilic chromatographic post to state the second chromatographic column.
5. the multi-dimensional chromatograph system as described in any one of Claims 1 to 4, it is characterised in that first chromatographic column and described
Two collectors are provided between second chromatographic column, one of them is used to the sample after the first chromatogram post separation being transferred to
Second chromatographic column is separated, and another is used to continue to collect the sample after the first chromatogram post separation, and two institutes
Collector is stated alternately to collect the sample after the first chromatogram post separation and be transferred to second chromatographic column to be divided
From.
6. multi-dimensional chromatograph system as claimed in claim 5, it is characterised in that also including switching valve, first chromatographic column, institute
Two collectors stated between the second chromatographic column and first chromatographic column and second chromatographic column are cut with described respectively
Valve connection is changed, the switching valve allows hand over state, so that two collectors alternately collect first chromatographic column point
Sample from after is simultaneously transferred to second chromatographic column to be separated.
7. multi-dimensional chromatograph system as claimed in claim 6, it is characterised in that the switching valve be two eight ways valves, two ten
Port valve is made up of two six-way valve connections.
8. multi-dimensional chromatograph system as claimed in claim 7, it is characterised in that described when the switching valve is two eight ways valves
Switching valve has eight ports, is followed successively by first port, second port, the 3rd port, the 4th port, fifth port, the 6th end
Mouth, the 7th port and the 8th port；
First chromatographic column connects with the first port, and second chromatographic column connects with the 7th port；Wherein one
The both ends of the individual collector connect with the second port and the 6th port respectively, the both ends of another collector
Connected respectively with the 4th port and the 8th port；The fifth port is used to discharge waste liquid；
The switching valve has two states and can switched between described two states；One of state is：Described
Single port connects with the second port, and the 3rd port connects with the 4th port, the fifth port and described the
Six ports connect, and the 7th port connects with the 8th port；Another state is：The first port and the 8th end
Mouth connection, the second port connect with the 3rd port, and the 4th port connects with the fifth port, and the described 6th
Port connects with the 7th port.
9. multi-dimensional chromatograph system as claimed in claim 7, it is characterised in that described when the switching valve is two ten-way valves
Switching valve has ten ports, is followed successively by first port, second port, the 3rd port, the 4th port, fifth port, the 6th end
Mouth, the 7th port, the 8th port, the 9th port and the tenth port；
First chromatographic column connects with the first port, and second chromatographic column connects with the 9th port；Wherein one
The both ends of the individual collector connect with the 3rd port and the tenth port respectively, the both ends of another collector
Connected respectively with the fifth port and the 8th port；6th port is used to discharge waste liquid；
The switching valve has two states and can switched between described two states；One of state is：Described
Single port connects with the tenth port, and the second port connects with the 3rd port, the 4th port and described the
Five ports connect, and the 6th port connects with the 7th port, and the 8th port connects with the 9th port；It is another
State is：The first port connects with the second port, and the 3rd port connects with the 4th port, and the described 5th
Port connects with the 6th port, and the 7th port connects with the 8th port, the 9th port and the described tenth
Port connects.
10. multi-dimensional chromatograph system as claimed in claim 7, it is characterised in that when the switching valve is made up of two six-way valves,
One of six-way valve has six ports, is followed successively by first port, second port, the 3rd port, the 4th port, fifth port
And the 6th port；Another six-way valve has six ports, is followed successively by the 7th port, the 8th port, the 9th port, the tenth end
Mouth, the tenth Single port and the tenth Two-port netwerk；
The second port connects with the 7th port, and the 4th port connects with the tenth Single port；Described first
Chromatographic column connects with the first port；Second chromatographic column connects with the fifth port；One of them described collector
Both ends connected respectively with the 3rd port and the 6th port, the both ends of another collector are respectively with described
Nine ports connect with the tenth Two-port netwerk；
The switching valve has two states and can switched between described two states；One of state is：Described
Single port connects with the 6th port, and the second port connects with the 3rd port, the 4th port and described the
Five ports connect, and the 7th port connects with the 8th port, and the 9th port connects with the tenth port, described
Tenth Single port connects with the tenth Two-port netwerk；Another state is：The first port connects with the second port, described
3rd port connects with the 4th port, and the fifth port connects with the 6th port, the 7th port with it is described
Tenth Two-port netwerk connects, and the 8th port connects with the 9th port, and the tenth port connects with the tenth Single port
It is logical.
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