CN206114669U - Immune colloidal gold reagent plate for detecting tripolycyanamide - Google Patents
Immune colloidal gold reagent plate for detecting tripolycyanamide Download PDFInfo
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- CN206114669U CN206114669U CN201621092245.2U CN201621092245U CN206114669U CN 206114669 U CN206114669 U CN 206114669U CN 201621092245 U CN201621092245 U CN 201621092245U CN 206114669 U CN206114669 U CN 206114669U
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- plate
- transparent cover
- detection
- tripolycyanamide
- pad
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Abstract
The utility model discloses an immune colloidal gold reagent plate for detecting tripolycyanamide. The device includes: the bottom plate is equipped with a three -dimensional translucent cover on, fixed surface installs a pair of lift cylinder on the bottom plate, and fixed mounting has the lifter plate on a pair of lift cylinder, and the lifter plate is located three -dimensional translucent cover, is equipped with the PVC bottom plate on the lifter plate, is equipped with one deck nitrocellulose membranes on the PVC bottom plate, and last detection line and the matter accuse line of being equipped with respectively of nitrocellulose membranes still is equipped with on the nitrocellulose membranes left side and glues gold combination pad, be equipped with square anchor clamps no. 1 on the inner wall of three -dimensional translucent cover left side, a square anchor clamps right side accompanies the sample pad, and the sample side of stacking up still is equipped with the reagent pipe, and the reagent pipe is fixed at three -dimensional translucent cover upper surface, be equipped with square anchor clamps no. 2 on the inner wall of three -dimensional translucent cover right side, two left sides of square anchor clamps accompany the pad that absorbs water, absorb water to fill up to be located the nitrocellulose membranes top, three -dimensional translucent cover upper surface is equipped with controller and attention device, the controller respectively with the electric signal connection of attention device and lift cylinder.
Description
Technical field
This utility model is related to a kind of agent plate of melamine residual in detection animal feed, milk and milk powder, especially
It is related to a kind of immune colloidal gold reagent plate of detection tripolycyanamide.
Background technology
Tripolycyanamide (melamine) is a kind of triazines nitrogen heterocyclic ring Organic substance, also known as melamine, cyanogen urine Disnalon (Ferrer)..Due to
The content of its nitrogen is high, tasteless low toxicity, adds existing food and feed industry protein content method of testing has certain defect,
Tripolycyanamide by illegal businessman be used as food additive, to lift food inspection in protein content index.But take the photograph for a long time
Enter this food containing tripolycyanamide, reproduction, the infringement of urinary system, bladder and kidney stone can be caused, or even can be further
Induction bladder cancer.Once there are many cause feedings and led by the feedstuff of the raw material production of tripolycyanamide pollution in March, 2007, the U.S.
Pet cat, Canis familiaris L. is caused to be poisoned to death event；And in part of in September, 2008, Chinese multiple brand baby milk powders are because containing tripolycyanamide
Cause child's renal calculuss.This sequence of events causes the extensive concern of domestic and international problem safe to food, and food and animal are raised
The detection of melamine residual in material has become necessary.
On October 7th, 2008, after " malicious milk powder " event, Ministry of Public Health, the Ministry of Industry and Information Technology, the Ministry of Agriculture, industry and commerce are total
Office, State Administration of Quality Supervision, Inspection and Quarantine combine and have printed and distributed the bulletin specified with regard to milk product and containing tripolycyanamide temporary control and education Limited Doses in dairy products.
Specify in bulletin：The Limited Doses of tripolycyanamide are 1mg/kg in milk power for infant and young children；Liquid milk (including raw milk), milk
The Limited Doses of tripolycyanamide are 2.5mg/kg in powder, other prescription emulsifiable powders；Tripolycyanamide in other food containing breast more than 15%
Limited Doses be 2.5mg/kg.
At present, for the main rationalization analytic process of the detection method of melamine residual and immunoassay.Physico-chemical analysis
Method, such as HPLC and LC-MS, with specificity it is good, accuracy rate is high the characteristics of, Shi Ge great testing agencies to detect sample confirm
Prefered method, but exist to equipment, environment, operant skill etc. require, it is required costly, be unfavorable for extensive screening sample,
Thus be not suitable for vast department of basic unit.Immunoassay, such as enzyme linked immunosorbent assay (ELISA), it is big with detection limit, operate phase
To it is simple the advantages of, be increasingly being used for the detection of antibiotic residues in animal-derived food, but ELISA method whole operation
Time remains a need for 1-2h, and need to use expensive special instruments and equipment, with certain limitation.
Food safety involves the interests of the state and the people, and develops a kind of test limit and meets the requirements, and favorable reproducibility, detection time is short,
Suitable field quick detection, technical products or the lower-cost quick detection reagent of instrument and equipment it is extremely urgent.
Utility model content
The purpose of this utility model is to provide a kind of sensitivity height, and specificity is good, easy to be quick, the three of low production cost
Poly cyanamid immune colloid gold quick detection reagent plate.
This utility model provides a kind of immune colloidal gold reagent plate of detection tripolycyanamide, including base plate, the base plate
A stereo transparent cover is provided with, plate upper surface is installed with a pair of lift cylinders, fixed peace on the pair of lift cylinder
Equipped with lifter plate, the lifter plate is located in stereo transparent cover, and lifter plate is provided with PVC base plates, and the PVC base plates are provided with one
Layer nitrocellulose filter, is respectively equipped with detection line and nature controlling line on the nitrocellulose filter, the detection line is located at nature controlling line
Left side, is additionally provided with glue gold pad on the nitrocellulose filter left side, the glue gold pad left side is close to PVC base plates；
Square fixture one is installed with the stereo transparent cover inner left wall, the right side of the square fixture one accompanies sample pad, institute
Sample pad is stated positioned at glue gold pad top, sample pad top is additionally provided with Reagent Tube, and the Reagent Tube is fixed on stereo transparent cover
Upper surface；Square fixture two is installed with the inwall of the stereo transparent cover right side, the left side of the square fixture two accompanies suction
Water cushion, the adsorptive pads are located at nitrocellulose filter top；The stereo transparent cover upper surface is provided with controller and attention device, institute
State controller to be connected with attention device and the lift cylinder signal of telecommunication respectively, the microprocessor in controller respectively with stereo transparent cover on
The light sensor one arranged in surface inner-wall and the connection of the signal of telecommunication of light sensor two, the light sensor one is located at detection
Directly over line, light sensor two is located at directly over nature controlling line.
It is preferred that being coated with tripolycyanamide carrier protein couplet thing on the nitrocellulose filter, tripolycyanamide is coupled
Carrier protein can be bovine serum albumin, ovalbumin and hemocyanin.
This utility model detectable plate, due to being provided separately for sample pad and adsorptive pads, so as to through repeated detection
Afterwards, the section components in whole detection plate need to be only changed, so as to significantly reduce cost；Light sensor one and light sensor
The setting of device two, such that it is able to easily by the microprocessor in the color reaction in detection line and nature controlling line to controller,
Comparing result is converted to the signal of telecommunication and is delivered in attention device by microprocessor after processing, and different comparing results are converted into
The different signals of telecommunication, so that attention device sends different sound, testing staff just can be square according to the sound that attention device sends
Just judge testing result, it is to avoid testing staff needs prolonged viewing agent plate, such that it is able to improving detection speed；
The setting of stereo transparent cover, can be effectively prevented from external environmental reagent, such that it is able to effectively improve detection structure；This
The integrated operation of utility model agent plate is simple, simple production process, easily promotes.
Description of the drawings
A kind of immune colloidal gold reagent plate structural representation of detection tripolycyanamide that Fig. 1 is provided for this utility model.
Description of reference numerals：
1- base plates, 2- lift cylinders, 3- lifter plates, 4-PVC base plates, 5- nitrocellulose filters, 5-1- detection lines, 5-2- matter
Control line, 6- glue gold pad, the square fixtures one of 7-, 8- sample pad, the square fixtures two of 9-, 10- adsorptive pads, 11- Reagent Tubes, 12-
Controller, 13- attention devices, 14- stereo transparent covers.
Specific embodiment
Below in conjunction with the accompanying drawings, a specific embodiment of the present utility model is described in detail, it is to be understood that this
The protection domain of utility model is not limited by specific embodiment.
As shown in figure 1, this utility model embodiment provides a kind of immune colloidal gold reagent plate of detection tripolycyanamide, bag
Base plate 1 is included, the base plate 1 is provided with a stereo transparent cover 14, and the upper surface of base plate 1 is installed with a pair of lift cylinders 2, described
Lifter plate 3 is installed with a pair of lift cylinders 2, the lifter plate 3 is located in stereo transparent cover 14, and lifter plate 3 is provided with
PVC base plates 4, the PVC base plates 4 are provided with one layer of nitrocellulose filter 5, and detection is respectively equipped with the nitrocellulose filter 5
Line 5-1 and nature controlling line 5-2, detection line 5-1 is located at nature controlling line 5-2 left sides, and the nitrocellulose filter 5 is additionally provided with left side
It is close to PVC base plates 4 in glue gold pad 6, the left side of glue gold pad 6；It is fixed in the inner left wall of stereo transparent cover 14
Square fixture 1 is installed, the right side of the square fixture 1 accompanies sample pad 8, and the sample pad 8 is located on glue gold pad 6
Side, the top of sample pad 8 is additionally provided with Reagent Tube 11, and the Reagent Tube 11 is fixed on the upper surface of stereo transparent cover 14；It is described three-dimensional saturating
Square fixture 29 is installed with 14 right side inwalls of bright cover, the left side of the square fixture 29 accompanies adsorptive pads 10, the suction
Water cushion 10 is located at the top of nitrocellulose filter 5；The upper surface of stereo transparent cover 14 is provided with controller 12 and attention device 13, described
Controller 12 is connected respectively with attention device 13 and the signal of telecommunication of lift cylinder 2, and the microprocessor in controller 12 is saturating with solid respectively
The light sensor one arranged on 14 upper surface inwalls of bright cover and the connection of the signal of telecommunication of light sensor two, the light sensor one
Directly over detection line 5-1, light sensor two is located at directly over nature controlling line 5-2.This utility model detectable plate, passes through
Controller controls the rise of lift cylinder, so that the glue gold pad and sample pad on PVC base plates is close to, nitrocellulose filter
It is close to adsorptive pads, subsequently extrudes after sample liquid from Reagent Tube, the color in detection line and nature controlling line can changes therewith, photosensitive
Color strong and weak signals in the transmission detection line of sensor one, the color strong and weak signals on the transmission nature controlling line of light sensor two, two
Contrast signal is converted into the signal of telecommunication and is delivered to attention device by individual color signal after the microprocessor contrast in controller is processed
In, so that attention device sends sound；When biography in the color strong and weak signals transmitted in light sensor one with light sensor two
The color strong and weak signals passed are the same, or the color strong and weak signals transmitted in dependent sensor one are transmitted more than in light sensor two
Color strong and weak signals, now attention device send a kind of sound, and then show in sample melamine concentration less than 2mg/kg or
Person is without melamine residual；When the little face with transmission in light sensor two of the color strong and weak signals transmitted in light sensor one
Color strength weak signal, now attention device sends another kind of sound, and then shows that melamine concentration is higher than 2mg/kg in sample；Work as light
The remote little color strong and weak signals with transmission in light sensor two of color strong and weak signals transmitted in dependent sensor one, now warn
Device sends again a kind of sound, and then shows that melamine concentration is very high in sample.The integrated operation of this utility model agent plate is simple,
Simple production process, easily promotes.
Further, tripolycyanamide carrier protein couplet thing is coated with the nitrocellulose filter 5, tripolycyanamide is coupled
Carrier protein can be bovine serum albumin, ovalbumin and hemocyanin.
In sum, the immune colloidal gold reagent plate of a kind of detection tripolycyanamide that this utility model embodiment is provided, bag
Base plate is included, the base plate is provided with a stereo transparent cover, and plate upper surface is installed with a pair of lift cylinders, the pair of liter
Lifter plate is installed with sending down the abnormal ascending QI cylinder, the lifter plate is located in stereo transparent cover, and lifter plate is provided with PVC base plates, described
PVC base plates are provided with one layer of nitrocellulose filter, and detection line and nature controlling line, the inspection are respectively equipped with the nitrocellulose filter
Survey line is located at nature controlling line left side, and glue gold pad, the glue gold pad left side are additionally provided with the nitrocellulose filter left side
It is close to PVC base plates；Square fixture one, the right side of the square fixture one are installed with the stereo transparent cover inner left wall
Sample pad is accompanied, the sample pad is located at glue gold pad top, and sample pad top is additionally provided with Reagent Tube, and the Reagent Tube is fixed
In stereo transparent cover upper surface；Square fixture two, the square fixture are installed with the inwall of the stereo transparent cover right side
Two left sides accompany adsorptive pads, and the adsorptive pads are located at nitrocellulose filter top；The stereo transparent cover upper surface is provided with control
Device and attention device, the controller is connected respectively with attention device and the lift cylinder signal of telecommunication, the microprocessor difference in controller
It is connected with the light sensor one and the signal of telecommunication of light sensor two that arrange on stereo transparent cover upper surface inwall, the photosensitive biography
Sensor one is located at directly over detection line, and light sensor two is located at directly over nature controlling line.This utility model detectable plate, due to
Sample pad and adsorptive pads are provided separately, so as to after repeated detection, need to only changing the section components in whole detection plate,
So as to significantly reduce cost；The setting of light sensor one and light sensor two, such that it is able to easily by detection line
In the microprocessor in the color reaction on nature controlling line to controller, microprocessor is changed comparing result after processing
It is delivered in attention device for the signal of telecommunication, different comparing results is converted into the different signals of telecommunication, so that attention device sends difference
Sound, testing staff just can easily judge testing result according to the sound that attention device sends, it is to avoid testing staff
Prolonged viewing agent plate is needed, such that it is able to improve detection speed；The setting of stereo transparent cover, can be effectively prevented from outer
Boundary's environmental pollution reagent, such that it is able to effectively improve detection structure；The integrated operation of this utility model agent plate is simple, production work
Skill is simple, easily promotes.
Disclosed above is only several specific embodiments of the present utility model, but, this utility model embodiment not office
It is limited to this, the changes that any person skilled in the art can think of should all fall into protection domain of the present utility model.
Claims (2)
1. a kind of immune colloidal gold reagent plate of detection tripolycyanamide, including base plate (1), it is characterised in that on the base plate (1)
A stereo transparent cover (14) is provided with, base plate (1) upper surface is installed with a pair of lift cylinders (2), the pair of lift cylinder
(2) lifter plate (3) is installed with, in stereo transparent cover (14), lifter plate (3) is provided with PVC to the lifter plate (3)
Base plate (4), the PVC base plates (4) are provided with one layer of nitrocellulose filter (5), are respectively equipped with the nitrocellulose filter (5)
Detection line (5-1) and nature controlling line (5-2), the detection line (5-1) is located at nature controlling line (5-2) left side, the nitrocellulose filter
(5) glue gold pad (6) is additionally provided with left side, glue gold pad (6) left side is close to PVC base plates (4)；It is described three-dimensional saturating
Square fixture one (7) is installed with bright cover (14) inner left wall, the right side of the square fixture one (7) accompanies sample pad (8),
The sample pad (8) is additionally provided with Reagent Tube (11), the Reagent Tube positioned at glue gold pad (6) top, sample pad (8) top
(11) it is fixed on stereo transparent cover (14) upper surface；Square fixture is installed with the inwall of stereo transparent cover (14) right side
Two (9), the left side of the square fixture two (9) accompanies adsorptive pads (10), and the adsorptive pads (10) are on nitrocellulose filter (5)
Side；Stereo transparent cover (14) upper surface is provided with controller (12) and attention device (13), the controller (12) respectively with police
Show device (13) and lift cylinder (2) signal of telecommunication connection, the microprocessor in controller (12) respectively with stereo transparent cover (14) on
The light sensor one arranged in surface inner-wall and the connection of the signal of telecommunication of light sensor two, the light sensor one is located at detection
Directly over line (5-1), light sensor two is located at directly over nature controlling line (5-2).
2. a kind of immune colloidal gold reagent plate of detection tripolycyanamide as claimed in claim 1, it is characterised in that the nitric acid
Tripolycyanamide carrier protein couplet thing is coated with cellulose membrane (5), the carrier protein for being coupled tripolycyanamide can be pure for Sanguis Bovis seu Bubali
Albumen, ovalbumin and hemocyanin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621092245.2U CN206114669U (en) | 2016-09-29 | 2016-09-29 | Immune colloidal gold reagent plate for detecting tripolycyanamide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621092245.2U CN206114669U (en) | 2016-09-29 | 2016-09-29 | Immune colloidal gold reagent plate for detecting tripolycyanamide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206114669U true CN206114669U (en) | 2017-04-19 |
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ID=58522158
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621092245.2U Expired - Fee Related CN206114669U (en) | 2016-09-29 | 2016-09-29 | Immune colloidal gold reagent plate for detecting tripolycyanamide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206114669U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141564A (en) * | 2018-11-02 | 2020-05-12 | 莱卡生物系统努斯洛赫有限责任公司 | Device, method and tissue processor for indicating the state of a solvent |
-
2016
- 2016-09-29 CN CN201621092245.2U patent/CN206114669U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141564A (en) * | 2018-11-02 | 2020-05-12 | 莱卡生物系统努斯洛赫有限责任公司 | Device, method and tissue processor for indicating the state of a solvent |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170419 Termination date: 20190929 |
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| CF01 | Termination of patent right due to non-payment of annual fee |