CN206104448U - Biochip self - cleaning device - Google Patents

Abstract

The utility model belongs to the technical field of biochip, a biochip self - cleaning device is related to. Including last box, lower box, the T montant that appears, gear, rack, hold -in range, gear rotor shaft, sleeve, the T gallows that appears, chip rack and washing liquid, T shape montant lower extreme is equipped with the gear, and the gear links to each other with motor I, and wheel and rack meshes mutually, and the rack is fixed on the horizontal pole of T shape montant, is equipped with the hold -in range on the rack, and the hold -in range both ends are equipped with the synchronizing wheel, and the hold -in range links to each other with motor II, cooperate with gear rotor shaft in the hold -in range bottom, gear rotor shaft and rack lower extreme sliding connection, gear rotor shaft be the by a small margin lift along with the lift of rack, the sleeve registrates with gear rotor shaft mutually, and gear rotor shaft descends the T shape hanger connected with the inversion, is equipped with the chip rack on the T shape gallows, and the chip rack is located the washing liquid. The utility model discloses simple and practical has realized the self - cleaning to biochip.

Description

A kind of biochip automatic clearing apparatuss

Technical field

This utility model belongs to biochip technology field, is related to a kind of biochip automatic clearing apparatuss.

Background technology

Biochip, also known as protein chip or gene chip, they originate from DNA hybridization probe technique and semi-conductor industry The crystallization that technology combines.The technology mean after a large amount of probe molecules are fixed on holder with fluorescently-labeled DNA or Other sample molecules（Such as albumen, the factor or small molecule）Hybridized, it is strong by the hybridization signal for detecting each probe molecule The quantity and sequence information of degree and then acquisition sample molecule.Biochip technology originates from making nucleic acid molecular hybridization.So-called biological core Piece refers generally to the biological information molecule that high density is fixed on mutual supporting dielectric（Such as genetic fragment, DNA fragmentation or polypeptide, egg White matter, glycan molecule, tissue etc.）Microarray hybridization cake core（micro-arrays）, the sequence of each molecule and position in array It is all known to put, and is pre-set sequence dot matrix.Micro-fluidic chip and liquid phase biochip are than microarray core The biochip new technique developed after piece, biochip technology are the substances of Systems biotechnology.Into 21 century, with Biotechnology is developed rapidly, and electronic technology and biotechnology combine brother --- the biological core of semiconductor chip of being born Piece, the life to us is brought a deepgoing revolution by this.This revolution can be all played for global sustainable development Immeasurable contribution.The cleannes of biochip have a great impact to the function of biochip, therefore, to biochip Cleaning sanitary equipment have very big requirement.Existing biological chip washing method mainly has two classes, and a class is cleaned using instrument, separately An outer class is washed using shaking table vibration.

Mainly there are two kinds using instrument clean：A kind of is special cleaning instrument, as Advalytix companies produce AdvaWash AW400 microscope slides wash instrument, and which can be cleaned to general wave carrier piece, and simultaneously four chips can be carried out clearly Wash, although which realizes the automatic cleaning of chip, can not meet some chips must be carried out under the conditions of uniform temperature clearly The requirement washed.Another is washed using the wash mill that hybridization instrument is carried, such as Amersham Biosciences The Lucidea SlidePro hybridization instruments of company's production, can hybridize six chips simultaneously, and its subsidiary cleaning is can use after hybridization Drying device carries out cleaning-drying to chip, but which is full automatic working, it is difficult to realize individually operated, is also unfavorable for large quantities of Amount washing, and there is dead volume in its chip in cleaning process, be especially directed to the larger chips of dot matrix scope such as express spectra more It is the not thorough phenomenon of dot matrix cleaning easily occur.

Washed using shaking table vibration, wherein shaking table is divided into common shaking table and shaking bath again.Cleanout fluid can pour plastics into In box or in beaker, chip is directly placed on the copper frame in plastic casing or in beaker and carries out vibration washing.Relative on copper frame Washing, washing of the chip in plastic casing are unfavorable for that high-volume is washed and concentrates centrifuge dripping.Chip is washed on copper frame in beaker When washing, as copper frame levels washing liquid flow velocity difference is increased, impact is produced on chip easily also, in beaker the amount of washing liquid is not in addition With the impact for cleaning speed than larger, this is greatly lowered the repeatability of chip cleaning.For both shaking tables The mode of washing, if in the case where chip needs two or more cleanout fluid to clean, its process for changing clothes liquid is also needed to artificially Manual operations, the artificial manual error brought more reduce the repeatability of chip cleaning.

Utility model content

This utility model solves biochip cleaning and is difficult, it is impossible to the technical barrier of automatization, discloses a kind of biology Chip automatic clearing apparatuss.

To solve above-mentioned technical barrier, employ the following technical solutions：

A kind of biochip automatic clearing apparatuss, including upper box, lower box, ┩ shape montants, gear, tooth bar, Timing Belt, Rotary gear shaft, sleeve, T-shaped suspension bracket, chip rack, motor I, motor II and cleanout fluid；It is provided with ┩ shapes to erect in the top box body Bar, gear, tooth bar and Timing Belt, ┩ shape montants lower end are provided with gear, and gear is connected with motor I, and wheel and rack is meshed, tooth Bar is fixed on the cross bar of ┩ shape montants, and tooth bar is provided with Timing Belt, and Timing Belt two ends are provided with synchronizing wheel, Timing Belt and motor II It is connected；The Timing Belt bottom is engaged with rotary gear shaft, and rotary gear shaft is connected with tooth bar lower slide, and rotary gear shaft is with tooth The lifting of bar and lift by a small margin；Sleeve, T-shaped suspension bracket, chip rack and cleanout fluid are provided with the nowel body；The sleeve With rotary gear shaft phase fit, rotary gear shaft is connected with inverted T-shaped suspension bracket, and T-shaped suspension bracket is provided with chip rack, and chip is placed Frame is located in cleanout fluid.

As preferred version of the present utility model, between the rotary gear shaft and sleeve, cushion is provided with, it is to avoid in cleaning During rotary gear shaft rock, be bolted between rotary gear shaft and T-shaped suspension bracket.

As preferred version of the present utility model, in the chip rack, 5-10 chip slot is provided with.The lower box With automatic temperature-adjusting function, the temperature of cleanout fluid can be adjusted as desired.

The beneficial effects of the utility model are：Engagement of this utility model by wheel and rack, realizes automatically adjusting The height of rotary gear shaft；Timing Belt is cooperated with the gear of rotary gear shaft, makes chip rack realize rotary cleaning, is discharged Centrifuge dripping can also be realized after cleanout fluid；This utility model is simple and practical, realizes the automatic cleaning to biochip.

Description of the drawings

Fig. 1 is structural representation of the present utility model；Wherein 1- lower boxes, 2- cleanout fluid, 3-T shape suspension brackets, 4- bolts, 5- Rotary gear shaft, 6- sleeves, 7- Timing Belts, 8- cross bars, 9- synchronizing wheels, 10- motors II, 11- tooth bars, 12- ┩ shape montants, 13- teeth Wheel, 14- motors, 15- upper boxes, 16- chip racks, 17- chips, 18- chip slots.

Specific embodiment

As shown in figure 1, a kind of biochip automatic clearing apparatuss, including upper box 15, lower box 1, ┩ shapes montant 12, tooth Wheel 13, tooth bar 11, Timing Belt 7, rotary gear shaft 5, sleeve 6, T-shaped suspension bracket 3, chip rack 16, motor I 14, II 10 He of motor Cleanout fluid 2；┩ shapes montant 12, gear 13, tooth bar 11 and Timing Belt 7 are provided with the upper box 15,12 lower end of ┩ shapes montant sets There is gear 13, gear 13 is connected with motor 14, and gear 13 is meshed with tooth bar 11, tooth bar 11 is fixed on the cross bar of ┩ shapes montant 12 On 8, tooth bar 11 is provided with Timing Belt 7, and 7 two ends of Timing Belt are provided with synchronizing wheel 9, and Timing Belt 7 is connected with motor II 10；The synchronization 7 bottoms of band are engaged with rotary gear shaft 5, and rotary gear shaft 5 is connected with 11 lower slide of tooth bar, and rotary gear shaft 5 is with tooth bar 11 Lift and lift by a small margin；Sleeve 6, T-shaped suspension bracket 3, chip rack 16 and cleanout fluid 2 are provided with the lower box 1；The set Cylinder 6 and 5 phase fit of rotary gear shaft, rotary gear shaft 5 and inverted connection, T-shaped suspension bracket 3 are provided with chip rack 16, and chip is put Put frame 16 to be located in cleanout fluid 2.Cushion is provided between the rotary gear shaft 5 and sleeve 6, it is to avoid turn in cleaning process middle gear Rocking for axle 5, is connected by bolt 4 between rotary gear shaft 5 and T-shaped suspension bracket 3.5-10 core is provided with the chip rack 16 Film trap 18.The lower box 1 has automatic temperature-adjusting function, and the temperature of cleanout fluid 2 can be adjusted as desired.

Concrete operations of the present utility model are as follows：Chip 17 is put in the chip slot 18 of chip rack 16, then will Chip rack 16 is hung on inverted T-shaped suspension bracket 3, is opened motor I 14, by the cooperation between gear 13 and tooth bar 11, is made 11 uniform descent of tooth bar, also declines in the drive lower gear rotating shaft 5 of tooth bar 11, when chip 17 is immersed in cleanout fluid 2, opens electricity Machine II 10, under the drive of Timing Belt 7,5 Periodic Rotating of rotary gear shaft starts the cleaning of chip 17；Cleaning is opened after terminating Chip rack 16 is risen to disengaging cleanout fluid 2, opens motor II 10, centrifuge dripping by motor I 14.

Claims (3)

1. a kind of biochip automatic clearing apparatuss, it is characterised in that：Including upper box（15）, lower box（1）, ┩ shape montants （12）, gear（13）, tooth bar（11）, Timing Belt（7）, rotary gear shaft（5）, sleeve（6）, T-shaped suspension bracket（3）, chip rack （16）, motor I（14）, motor II（10）And cleanout fluid（2）；The upper box（15）┩ shape montants are provided with inside（12）, gear （13）, tooth bar（11）And Timing Belt（7）, ┩ shape montants（12）Lower end is provided with gear（13）, gear（13）With motor I（14）Phase Even, gear（13）With tooth bar（11）It is meshed, tooth bar（11）It is fixed on ┩ shape montants（12）Cross bar（8）On, tooth bar（11）On It is provided with Timing Belt（7）, Timing Belt（7）Two ends are provided with synchronizing wheel（9）, Timing Belt（7）With motor II（10）It is connected；The Timing Belt （7）Bottom and rotary gear shaft（5）It is engaged, rotary gear shaft（5）With tooth bar（11）Lower slide connects；The lower box（1）It is interior It is provided with sleeve（6）, T-shaped suspension bracket（3）, chip rack（16）And cleanout fluid（2）；The sleeve（6）With rotary gear shaft（5）It is nested Close, rotary gear shaft（5）With inverted T-shaped suspension bracket（3）Connection, T-shaped suspension bracket（3）It is provided with chip rack（16）, chip placement Frame（16）Positioned at cleanout fluid（2）It is interior.

2. biochip automatic clearing apparatuss as claimed in claim 1, it is characterised in that：The rotary gear shaft（5）With sleeve （6）Between be provided with cushion, rotary gear shaft（5）With T-shaped suspension bracket（3）Between pass through bolt（4）Connection.

3. biochip automatic clearing apparatuss as claimed in claim 1, it is characterised in that：The chip rack（16）Inside set There is 5-10 chip slot（18）.