CN206096151U - Detect fluorescence test paper strip of tetracycline class and sulfanilamide (SN) class antibiotic simultaneously - Google Patents
Detect fluorescence test paper strip of tetracycline class and sulfanilamide (SN) class antibiotic simultaneously Download PDFInfo
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- CN206096151U CN206096151U CN201620578448.6U CN201620578448U CN206096151U CN 206096151 U CN206096151 U CN 206096151U CN 201620578448 U CN201620578448 U CN 201620578448U CN 206096151 U CN206096151 U CN 206096151U
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Abstract
The utility model discloses an it detects technical field, concretely relates to detect fluorescence test paper strip of tetracycline class and sulfanilamide (SN) class antibiotic simultaneously to belong to fluorescence immunity chromatography. Fluorescence test paper strip includes PVC bottom plate, sample pad, reacts the membrane, paper absorbs water, wherein, the sample wafer pad, react the membrane and absorb water paper and be and be located the PVC bottom plate, and sample pad and the paper that absorbs water be located respectively the reaction membrane both sides and with the contact of reaction membrane, does the detect reagent who adopts when fluorescence test paper strip uses include tetracycline class antibiotic monoclonal antibody tombarthite europium red fluorescence tag and sulfanilamide (SN) class antibiotic monoclonal antibody tombarthite europium red fluorescence tag, and the freeze -drying is in plastics reagent bucket. Fluorescence test paper strip have portable good, sensitivity is high, check -out time is short, can detect characteristics such as tetracycline class and sulfanilamide (SN) class antibiotic simultaneously, exerts the important function in the multiclass antibiotic detects simultaneously.
Description
Technical field
This utility model belongs to fluorescence immune chromatography detection technique field, and in particular to it is a kind of detect simultaneously Tetracyclines and
The fluorescent test paper strip of sulfa antibiotics.
Background technology
Antibiotic has the ability for killing microorganism, and in modern livestock and poultry breeding industry, antibiotic is widely used, such as Fourth Ring
Plain class antibiotic is used to promote growth of animals or poultry, sulfa antibiotics to be used to prevent and treat the infection class disease such as diarrhoea.Due to anti-
Raw element action effect is good, instant effect, and many Producers tend to be excessively used antibiotic.Research shows：Unreasonable use antibiosis
Plain medicine, does not only result in residual antibiotic in animal food, and the Animal fecal pollution for discharging enter after environment can to ambient soil,
Environment water causes antibiotic to pollute, and in the drinking water source of China many places has antibiotic to detect.Human body intake for a long time is containing anti-
The food of raw element can destroy the environment of healthy human body normal flora, cause immunocompetence to decline.For long term monitoring food, environment
The residual quantity of middle antibiotic, to meet MRL standard, plant and environmental monitoring department etc. are all actively finding accurate
Really, quick, feasible detection method.
Tetracycline antibiotics (Tetracyclines) include chlortetracycline (chlotetracycline), oxytetracycline
(oxytetracycline), tetracycline (tetracycline) and semi-synthetic derivative methacycline, doxycycline, diformazan
Amido tetracycline etc., its structure basic framework containing aphthacene.Tetracycline antibiotics have broad spectrum activity, are widely used in poultry
Aquaculture and clinical treatment；Wherein, tetracycline, chlortetracycline, oxytetracycline are most widely used.Sulfa antibiotics
(Sulfonamides) refer to the class antibiotic with P-aminobenzene-sulfonamide structure, up to thousands of kinds of species, and synthesize simple,
Volume of production is big, low price.As there is certain toxicity to be gradually backed out the clinical treatment of human body for which, but it is still widely used for poultry
Aquaculture, is one of most widely used veterinary drug antibiotic.
At present for the detection method of tetracycline antibiotics and sulfa antibiotics substantially has：Microbial method, enzyme-linked exempt from
Epidemic disease method, Ultra Performance Liquid Chromatography-mass spectrum/MS, high performance capillary electrophoresis, fluorimetry etc..Veterinary drug inspection at present
Survey object and be substantially similar antibiotic medicine, for while detecting that the technique study of multiclass antibiotic is few, it is impossible to meet veterinary drug
The ever-increasing present situation of species.High, the with a high credibility detection method of detection field sensitivity is liquid chromatograph mass spectrography at present
Method, but the method needs expensive instrument, the experimenter of technology maturation, at the same also need to the pre-treatment of large amount of complex and it is long when
Between detection time, be not suitable for the screening of field quick detection and batch samples.Realize quick detection and batch samples
Rapid screening for monitoring food and environment in antibiotic residual it is significant.
Utility model content
The purpose of this utility model is to provide a kind of while detecting the fluorescent test paper of Tetracyclines and sulfa antibiotics
Bar, the technical scheme taken are as follows：
It is a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, the fluorescent test paper strip includes：PVC
Base plate 1, sample pad 2, reaction film 3, absorbent paper 4；Wherein, print pad 2, reaction film 3 and absorbent paper 4 is positioned at PVC base plates 1
On, and sample pad 2 and absorbent paper 4 respectively positioned at reaction film 3 both sides and contact with reaction film 3；
The first detection line 5, the second detection line 6 and nature controlling line 7 are fixed with the reaction film 3；First detection line 5, second
Detection line 6 and nature controlling line 7 are parallel to each other and with the length of fluorescent test paper strip to vertical, and the first detection line 5 is near sample pad 2 one
End, nature controlling line 7 are located between the first detection line 5 and nature controlling line 7 near 4 one end of absorbent paper, the second detection line 6.
Tetracycline antibiotics hapten-carrier protein conjugate, second inspection are coated with first detection line 5
Sulfa antibiotics hapten-carrier protein conjugate is coated with survey line 6, sheep anti mouse is coated with the nature controlling line 7 anti-
Body.
The nature controlling line 7 away from reaction film 3 be connected with absorbent paper 4 one end spacing be 2-3mm；Second detection line 6 is away from Quality Control
The spacing of line 7 is 4-6mm；Spacing of first detection line 5 away from the second detection line 6 is 4-6mm.
Described sample pad 2 is glass fibre, and the reaction film 3 is nitrocellulose filter.
The beneficial effects of the utility model are：
1st, sensitivity is high：Detectable used by the test strips is anti-with rare-earth europium red fluorescence nano material labelling antibiotic
Body, can under 365nm ultraviolet lights direct observed result, due to the unique optical characteristics of rare-earth europium red fluorescence nano material, make
The detection of tetracycline antibiotics and sulfa antibiotics is greatly improved compared to colloidal gold labeled monoclonal antibody detection sensitivity, minimum
Can detect that the trace residue of nanogram level.The fluorescent test paper strip is respectively tetracycline to the detection sensitivity of tetracycline medication
100ng/l, oxytetracycline 100ng/l, chlortetracycline 100ng/l, are 1000ng/l to sulfa drugss sensitivity.
2nd, it is easy to operate quick：During using the fluorescent test paper strip detection sample, only testing sample need to be added containing detection
In the plastics reagent barrel of reagent, after mixing, 5min is incubated, then the detectable after incubation is inserted in test strips sample pad one end
In, result is observed under 365nm after 5min, without the need for being diluted to testing sample during detection.
3rd, while detecting, testing result is intuitively accurate：Whether testing result is developed the color with two detection lines and a nature controlling line
As negative and positive mark.If the first detection line does not develop the color, in representing testing sample, tetracycline antibiotics content is not less than
Lowest detectable limit of the test strips to tetracycline antibiotics；Colour developing is then represented less than lowest detectable limit.If the second detection line does not show
Color, in representing testing sample, sulfa antibiotics content is not less than lowest detectable limit of the test strips to sulfa antibiotics；Colour developing
Then represent less than lowest detectable limit.Therefore two class antibiotic can be detected simultaneously, and visual result accurately, is difficult erroneous judgement.
4th, it is easy to popularization and application on a large scale：The fluorescent test paper strip is simple to operate, meets making for different field level personnel
With needs, such as customs quarantine control department, environmental quality monitoring, food quality monitoring etc., with very big market prospect and economy, society
Can benefit.
Description of the drawings
Fig. 1 is the structural representation of the fluorescent test paper strip.
Fig. 2 is the top view of the fluorescent test paper strip.
Fig. 3 is testing result schematic diagram of the shown fluorescent test paper strip to testing sample.
Specific embodiment
With reference to the accompanying drawings and examples this utility model is described further, but protection domain of the present utility model is not
It is limited to this.
It is a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, the fluorescent test paper strip includes：PVC
Base plate 1, sample pad 2, reaction film 3, absorbent paper 4；Wherein, print pad 2, reaction film 3 and absorbent paper 4 is respectively positioned on PVC base plates 1,
And sample pad 2 and absorbent paper 4 are contacted positioned at the both sides of reaction film 3 and with reaction film 3 respectively；
The first detection line 5, the second detection line 6 and nature controlling line 7 are fixed with the reaction film 3；First detection line 5, second
Detection line 6 and nature controlling line 7 are parallel to each other and with the length of fluorescent test paper strip to vertical, and the first detection line 5 is near sample pad 2 one
End, nature controlling line 7 are located between the first detection line 5 and nature controlling line 7 near 4 one end of absorbent paper, the second detection line 6；
Tetracycline antibiotics hapten-carrier protein conjugate, second inspection are coated with first detection line 5
Sulfa antibiotics hapten-carrier protein conjugate is coated with survey line 6, sheep anti mouse is coated with the nature controlling line 7 anti-
Body.
The nature controlling line 7 away from reaction film 3 be connected with absorbent paper 4 one end spacing be 2-3mm；Second detection line 6 is away from Quality Control
The spacing of line 7 is 4-6mm；Spacing of first detection line 5 away from the second detection line 6 is 4-6mm.
Described sample pad 2 is glass fibre, and the reaction film is nitrocellulose filter.
Embodiment 1：The preparation of detectable monoclonal antibody-rare-earth europium red fluorescence label
Detectable used in the fluorescent test paper strip includes that tetracycline antibiotics monoclonal antibody-rare-earth europium redness is glimmering
Signal thing and sulfa antibiotics monoclonal antibody-rare-earth europium red fluorescence label, preparation method is：
The rare-earth europium red fluorescent microspheres of about 100 μ L are added in the PBS buffer solution of the NHS and EDC of 0.1mol/L, so
After be separately added into sulfonamides monoclonal antibody and Tetracyclines monoclonal antibody, under room temperature stir 4 hours, add BSA to its end
Concentration is 1%, stands 10min, is washed twice with PBS buffer solution after centrifugation, then resuspended with PBS solution, obtains Tetracyclines
Antibiotic monoclonal antibody-rare-earth europium red fluorescence label and sulfa antibiotics monoclonal antibody-rare-earth europium red fluorescence
Label, puts 4With.
Embodiment 2：The assembling of the fluorescent test paper strip of Tetracyclines and sulfa antibiotics is detected simultaneously
1) preparation of sample pad
Glass fibre membrane is cut into after strip, containing 1%BSA, is impregnated with the 0.02M PBS of 0.5% polysorbas20,60 DEG C are done
Dry 1h, is stored in standby in exsiccator.
2) preparation of reaction film
With PBS by sulfa antibiotics hapten-carrier protein conjugate and tetracycline antibiotics hapten-
Carrier protein couplet thing is diluted to 0.4mg/ml, and sheep anti-mouse antibody is diluted to 0.63mg/ml；Nitrocellulose filter cutting
Afterwards, the tetracycline antibiotics hapten-carrier protein conjugate that 2 μ L have diluted is coated with the first detection line, in the second inspection
2 μ L sulfa antibiotics hapten-carrier protein conjugates are coated with survey line, the goat-anti that 2 μ L have diluted is coated with nature controlling line
Murine antibody.
3) assembling of fluorescent test paper strip
Paste sample pad, reaction film and the absorbent paper for preparing on PVC base plates successively；The end of sample pad and reaction
Film top is connected, and reaction film end is connected with absorbent paper top, is assembled into fluorescent test paper strip.
Embodiment 4：The use of the fluorescent test paper strip of Tetracyclines and sulfa antibiotics is detected simultaneously
Used detectable includes tetracycline antibiotics monoclonal antibody-rare-earth europium red fluorescence label and sulphur
Amine antibiotic monoclonal antibody-rare-earth europium red fluorescence label, and lyophilizing is in plastics reagent barrel；
Testing sample is added in the plastics reagent barrel containing detectable, after mixing, 5min is incubated, then by test strips sample
In detectable after the insertion incubation of pad one end, after 5min, result is observed under 365nm；
In testing sample, sulfa antibiotics content is not less than lowest detection line, the sulfa antibiotics in testing sample with
Sulfa antibiotics monoclonal antibody in detectable-rare-earth europium red fluorescence label is combined, in the fluorescent-labeled antibody
Antigen binding site closing, in the second detection line, coated sulfa antibiotics hapten-carrier protein conjugate can not be with
Fluorescent-labeled antibody is combined, and fluorescence can not be sent under ultraviolet light 365nm；In testing sample, tetracycline antibiotics content is not low
In lowest detection line, the tetracycline antibiotics in testing sample and the tetracycline antibiotics monoclonal anti in detectable
Body-rare-earth europium red fluorescence label is combined, the antigen binding site closing in the fluorescent-labeled antibody, is wrapped in the first detection line
The tetracycline antibiotics hapten-carrier protein conjugate of quilt can not be combined with fluorescent-labeled antibody, under ultraviolet light 365nm
Fluorescence can not be sent.
When sulfa antibiotics in testing sample and tetracycline antibiotics are below the i.e. double-negative result of lowest detection line
When, the first detection line and the second detection line occur red stripes under ultraviolet light 365nm, as shown in S1 in Fig. 3；When treating test sample
In product, tetracycline antibiotics are not less than lowest detection line, when sulfa antibiotics are less than lowest detection line, in ultraviolet light 365nm
Lower first detection line occurs without red stripes, when red stripes occurs in the second detection line, as shown in S2 in Fig. 3；Work as testing sample
Middle tetracycline antibiotics are less than lowest detection line, and when sulfa antibiotics are not less than lowest detection line, the first detection line occurs
Red stripes, the second detection line occur without red stripes, as shown in S3 in Fig. 3；When tetracycline antibiotics in testing sample and
Sulfa antibiotics be not less than lowest detection line i.e. double positive findingses when, the first detection line and the second detection line are in ultraviolet light
Red stripes are occurred without under 365nm, as shown in S4 in Fig. 3；The above results judge red stripes occur as front with nature controlling line
Carry.
Nature controlling line occurs without red stripes, testing result mistake, as shown in ER in Fig. 3 under ultraviolet light 365nm.May be former
Because test strips are expired or detectable rotten, detection should be stopped immediately, change test strips and detectable.
Claims (4)
1. a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, it is characterised in that the fluorescent test paper
Bar includes：PVC base plates (1), sample pad (2), reaction film (3), absorbent paper (4)；Wherein, sample pad (2), reaction film (3) and suction
Water paper (4) is respectively positioned on PVC base plates (1), and sample pad (2) and absorbent paper (4) respectively positioned at reaction film (3) both sides and with it is anti-
Film (3) is answered to contact；
The first detection line (5), the second detection line (6) and nature controlling line (7) are fixed with the reaction film (3)；First detection line
(5), the second detection line (6) and nature controlling line (7) are parallel to each other and with the length of fluorescent test paper strip to vertical, and the first detection line (5)
Near sample pad (2) one end, nature controlling line (7) near absorbent paper (4) one end, the second detection line (6) positioned at the first detection line (5) and
Between nature controlling line (7).
2. according to claim 1 a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, which is special
Levy and be, on first detection line (5), be coated with tetracycline antibiotics hapten-carrier protein conjugate, described second
Sulfa antibiotics hapten-carrier protein conjugate is coated with detection line (6), on the nature controlling line (7), goat-anti is coated with
Mus anti antibody.
3. according to claim 1 a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, which is special
Levy and be, the nature controlling line (7) away from reaction film (3) be connected with absorbent paper (4) one end spacing be 2-3mm；Second detection line (6)
Spacing away from nature controlling line (7) is 4-6mm；Spacing of first detection line (5) away from the second detection line (6) is 4-6mm.
4. according to claim 1 a kind of while detecting the fluorescent test paper strip of Tetracyclines and sulfa antibiotics, which is special
Levy and be, described sample pad (2) is glass fibre, the reaction film (3) is nitrocellulose filter.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620578448.6U CN206096151U (en) | 2016-06-14 | 2016-06-14 | Detect fluorescence test paper strip of tetracycline class and sulfanilamide (SN) class antibiotic simultaneously |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107462715A (en) * | 2017-07-31 | 2017-12-12 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | A kind of carbofuran and Mobucin duplex inspection Immunofluorescence test paper strip and kit and its application |
| CN108535486A (en) * | 2018-02-11 | 2018-09-14 | 北京华海骏业科技发展有限公司 | A kind of chloramphenicol immunofluorescence assay method based on europium label |
| CN109813907A (en) * | 2017-11-22 | 2019-05-28 | 中国科学院上海生命科学研究院 | A kind of method that can detect simultaneously sulfamido and Tetracyclines |
| CN109870574A (en) * | 2017-12-05 | 2019-06-11 | 中国科学院上海生命科学研究院 | Extract the kit and detection method of antibiotic residue in food |
| CN110672843A (en) * | 2019-10-30 | 2020-01-10 | 南京海关动植物与食品检测中心 | Quintuplet time-resolved fluoroimmunoassay test strip for quantitatively determining sulfonamides |
| CN110873794A (en) * | 2018-08-29 | 2020-03-10 | 中国农业大学 | High-flux ultrasensitive double-label time-resolved immunochromatographic test strip and application thereof |
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| CN111198173A (en) * | 2020-01-14 | 2020-05-26 | 潍坊科技学院 | Preparation method of fluorescent test strip for environmental monitoring |
| CN111829996A (en) * | 2020-06-23 | 2020-10-27 | 江苏大学 | Preparation method of lanthanide metal organic framework test strip for visually detecting tetracycline antibiotics |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107462715A (en) * | 2017-07-31 | 2017-12-12 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | A kind of carbofuran and Mobucin duplex inspection Immunofluorescence test paper strip and kit and its application |
| CN107462715B (en) * | 2017-07-31 | 2018-10-02 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | A kind of carbofuran and Mobucin duplex inspection Immunofluorescence test paper strip and kit and its application |
| CN109813907A (en) * | 2017-11-22 | 2019-05-28 | 中国科学院上海生命科学研究院 | A kind of method that can detect simultaneously sulfamido and Tetracyclines |
| CN109870574A (en) * | 2017-12-05 | 2019-06-11 | 中国科学院上海生命科学研究院 | Extract the kit and detection method of antibiotic residue in food |
| CN108535486A (en) * | 2018-02-11 | 2018-09-14 | 北京华海骏业科技发展有限公司 | A kind of chloramphenicol immunofluorescence assay method based on europium label |
| CN110873794A (en) * | 2018-08-29 | 2020-03-10 | 中国农业大学 | High-flux ultrasensitive double-label time-resolved immunochromatographic test strip and application thereof |
| CN110873793A (en) * | 2018-08-29 | 2020-03-10 | 中国农业大学 | High-flux ultrasensitive fluorescent gold nano-cluster immunochromatographic test strip and application thereof |
| CN110672843A (en) * | 2019-10-30 | 2020-01-10 | 南京海关动植物与食品检测中心 | Quintuplet time-resolved fluoroimmunoassay test strip for quantitatively determining sulfonamides |
| CN111198173A (en) * | 2020-01-14 | 2020-05-26 | 潍坊科技学院 | Preparation method of fluorescent test strip for environmental monitoring |
| CN111829996A (en) * | 2020-06-23 | 2020-10-27 | 江苏大学 | Preparation method of lanthanide metal organic framework test strip for visually detecting tetracycline antibiotics |
| CN111829996B (en) * | 2020-06-23 | 2023-02-17 | 江苏大学 | Preparation method of lanthanide metal organic framework test strip for visually detecting tetracycline antibiotics |
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