CN207352021U - Furaxone metabolite quantum dot fluorescence test card - Google Patents

Abstract

The utility model discloses a kind of Furaxone metabolite quantum dot fluorescence test card, belong to the detection field of Furaxone metabolite, including by deck and the get stuck plastic body formed, block and test paper, deck both ends are equipped with the card column of positioning test paper, get stuck and the raised line of compacting test paper is useful for equipped with the cutting ferrule and ring cloth with the one-to-one corresponding grafting of two card columns；Test paper is quantum dot fluorescence test strips, is formed by overlapping the sample pad of connection, label pad, reaction film, water absorption pad successively, and sample pad stretches out plastic body suction side, and reaction film is equipped with detection line and nature controlling line；Get stuck and be equipped with observation window；Deck and air hole is equipped with getting stuck.This test card has cheap, it is easy to use, high sensitivity, quick and precisely, stability it is good, without other instruments and specialty personnel, can be with Furaxone metabolite the features such as, as a result judge it is intuitive and reliable, suitable for the remaining on-site supervision of Furaxone metabolite in animal derived food and the examination of great amount of samples.

Description

Furaxone metabolite quantum dot fluorescence test card

Technical field

The utility model belongs to the detection technique field of Furaxone metabolite, and in particular to a kind of Furaxone metabolite Quantum dot fluorescence test card.

Background technology

Furazolidone (Furazolidone) is Nitrofuran antibiotics, has stronger antibacterial effect, it is to most The pathogen such as number gram-positive bacteria, Gram-negative bacteria, protozoon have killing effect, because price is relatively low and effect is good, therefore In livestock and poultry and aquaculture using upper very extensive.But it has poultry certain toxicity, and have potential carcinogenic and lure Lead the material that organism produces mutation.Furazolidone can rapid metabolization in animal body, its metabolite 3-amino -2- oxazolidones (3-Amino-2-oxazolidinone, AOZ) can combine to form stable compound with protein, these metabolites can be Discharge and be absorbed by the body from protein under the acid condition of the gastric juice of the mankind, so as to be caused to health serious Harm.But study for a long period of time proof, Cistofuran metabolite and Furaxone metabolite have larger toxicity, can induce body gene Mutation, monster, cancer, therefore the great attention of people is caused, it is multinational to have prohibited such medicine in treatment and feed Use.Nineteen ninety-five, European Union provided against using itrofurans antibacterials comprehensively, the itrofurans in animal derived food The limit value of medicine and metabolite residue, which must not be, to be detected.The U.S. is completely forbidden Nitrofuran metabolites for food-borne within 2002 Animal.2004 food and drug administration (FDA) disclose forbid using in import animal derived food 11 kinds List of substance, including nitrofurazone and furazolidone.The Ministry of Agriculture of China issues No. 193 bulletins in April, 2002, discloses 《Food animal disables veterinary drug and other compound inventories》, it is specified that forbid using itrofurans antibiosis in all food animals Aquatic products Nitrofuran metatolites are included residual Supervisory Surveillance Program in 2003 by element again.

Currently used for detection furazolidone and its Metabolic residue thing main method have spectrophotometry, liquid chromatography, Liquid chromatography-mass spectrometry, Liquid Chromatography-Tandem Mass Spectrometry, enzyme-linked immunization etc., the expensive instrument of above-mentioned detection method needs, Sample pre-treatments are cumbersome, reagent consumption is big, detection time is long, it is necessary to the shortcomings of the experimenter of technology maturation, it is difficult to meet Quick, simple and direct, scene the requirement of modern measure.

Utility model content

In view of this, the purpose of this patent is to provide a kind of Furaxone metabolite quantum dot fluorescence test card, has Detection sensitivity is high, easy to operate, the time is short, stability is good, the personnel without other instruments and specialty, as a result judges directly perceived accurate Really, it is suitable for the screening of field quick detection and batch samples.

To reach above-mentioned purpose, the utility model provides following technical solution：

The utility model provides a kind of Furaxone metabolite quantum dot fluorescence test card, including plastic body, is sheathed on Block on plastic body suction side and the test paper in plastic body, the plastic body is by deck and is covered on deck On the composition that gets stuck, the deck is equipped with towards the corresponding surface both ends got stuck to be used to position the card column of test paper, and test paper is passed through by card column Wear on the bottom plate for being placed on deck；It is described get stuck towards deck corresponding surface be equipped with two card columns correspond grafting card Set and ring are furnished with the raised line with the matched compacting test paper of bottom plate；The test paper is quantum dot fluorescence test strips, by overlapping successively The sample pad of connection, label pad, reaction film, water absorption pad composition, the sample pad stretch out plastic clip away from label pad away from end Body suction side, and the circular hole passed through for card column is equipped with sample pad, water absorption pad, the reaction film is equipped with detection line and matter Control line；The observation window for being equipped with the corresponding position of reaction film and being able to observe that detection line and nature controlling line that gets stuck；It is described Deck and get stuck with being equipped with air hole on the corresponding position of absorption pad.

Further, the card column outer contour has the cross-sectional structure identical with the cutting ferrule Internal periphery, the cross section Structure is cylindrical type, square, D fonts or cross.

Further, the card column and cutting ferrule tight fit.

Further, the plastic body suction side petition warhead structure, for grafting or is screwed on the type of attachment of block.

Further, the sample pad, label pad and water absorption pad deviating from face covered with rubber protective layer away from bottom plate.

Further, the width that laminates of the sample pad and label pad is 2mm, and the width that laminates of absorption pad and reaction film is 2mm。

Further, quantum dot-labeled anti-Furaxone metabolite monoclonal antibody is perfused with the label pad.

Further, the detection line is to spray or be printed with Furaxone metabolite-carrier protein couplet on reaction film The detection line T lines of thing solution, the nature controlling line are the nature controlling line C line for spraying or being printed with sheep anti-mouse igg solution on reaction film, And the spacing between detection line T lines and nature controlling line C line is 4mm, detection line T lines are arranged close to the side of sample pad.

Further, described get stuck is additionally provided with detection line trace " C " corresponding with detection line T lines on the side of observation window With nature controlling line trace " T " corresponding with nature controlling line C line.

Further, sample pad and label pad are hard glass fiber, and reaction film is nitrocellulose filter, and bottom plate is moulded for hard Adhesive tape or the cardboard bar not absorbed water, water absorption pad are the pure white filter paper made of plant long fibre is raw material.

Compared with prior art, its remarkable advantage is the utility model：

1st, high sensitivity；For the utility model using quantum dot as label, quantum dot has unique better optical property：It is glimmering Light luminous efficiency is high, and exciting line scope is wide, can " unitary excites, polynary transmitting ", spectral line of emission narrow range and symmetrical, photobleaching Speed is slow, and fluorescence lifetime length, particle diameter is close with biomolecule, energy multifunction after surface modification, the amount of different-grain diameter and species The characteristic wavelength fluorescence spectrum that sub- azeotropic mixture produces does not overlap, and is very suitable for sample trace analysis, due to above-mentioned unique light Chemical property, improves detection sensitivity, which is 1ng/mL, detection sensitivity More traditional colloidal gold strip method improves about 10 times.

2nd, it is easy to operate quick：Using during the test card without other any reagents, as long as will be after processing, by test paper card insertion Enter sample it is molten in, 3-5min can observe in the UV lamp result or naked eyes directly observation.

3rd, show that testing result is intuitively accurate：Whether test card is developed the color as negative and positive standard using detection line, with The presence or absence of nature controlling line judges whether test card fails；Do not develop the color at test card detection line, nature controlling line C colour developing when, represent detection Furaxone metabolite content is greater than or equal to the detection sensitivity of test card in sample；At test card detection line and nature controlling line During place's colour developing, then it represents that the content of Furaxone metabolite is greater than or equal to the sensitivity of test card；When the nature controlling line of test card When place does not develop the color, detection line is regardless of whether colour developing all represents test card failure.Therefore visual result is accurate, is not easy to judge by accident.

4th, cost is low, is easy to promote and apply on a large scale：Not only detection speed is fast for the test card of the utility model, operation letter It is single, without using other instruments, meet the use needs of different field level personnel, such as foods supervision department, veterinary drug supervision department Door, customs quarantine control department, environmental quality monitoring etc., have very big market prospects and economical, societal benefits.

Other advantages, target and feature of the utility model will be explained in the following description to a certain extent State, and to a certain extent, based on will be apparent to those skilled in the art to investigating hereafter, or Person can be instructed from the practice of the utility model.The target of the utility model and other advantages can be said by following Bright book is realized and obtained.

Brief description of the drawings

In order to make the purpose of this utility model, technical solution and advantage clearer, below in conjunction with attached drawing to this practicality It is new to be preferably described in detail, wherein：

Fig. 1 is the overall positive structure schematic of Furaxone metabolite quantum dot fluorescence test card；

Fig. 2 is the overall structure schematic diagram of Furaxone metabolite quantum dot fluorescence test card；

Fig. 3 is the deck internal structure schematic diagram of Furaxone metabolite quantum dot fluorescence test card；

Fig. 4 is the internal structure schematic diagram that gets stuck of Furaxone metabolite quantum dot fluorescence test card；

Fig. 5 is the capping structure schematic diagram of Furaxone metabolite quantum dot fluorescence test card.

Embodiment

Illustrate the embodiment of the utility model below by way of specific instantiation, those skilled in the art can be by this theory Content disclosed by bright book understands other advantages and effect of the utility model easily.The utility model can also be by addition Different embodiments are embodied or practiced, and the various details in this specification can also be based on different viewpoints with answering With carrying out various modifications or alterations under the spirit without departing from the utility model.It should be noted that institute in following embodiments The diagram of offer only illustrates the basic conception of the utility model in a schematic way, in the case where there is no conflict, following embodiments and Feature in embodiment can be mutually combined.

Please refer to Fig.1-5, the element numbers in attached drawing represent respectively：1- blocks, 2- plastic bodies, 3- test paper, 4- decks, 5- gets stuck, 6- cutting ferrules, 7- card columns, 8- raised lines, 9- suction sides, 10- sample pads, 11- bottom plates, 12- label pads, 13- reaction films, 14- water absorption pads, 15- detection lines, 16- nature controlling lines, 17- observation windows, 18 air holes.

Embodiment is substantially as shown in drawings：The present embodiment provides a kind of Furaxone metabolite quantum dot fluorescence test card, Including plastic body 2, the block 1 being sheathed on 2 suction side of plastic body and the test paper 3 in plastic body 2, plastic clip Body 2 is by deck 4 and is covered on getting stuck on deck 4 and 5 forms, and deck 4 is equipped with towards the 5 corresponding surface both ends of getting stuck and is used to position examination The card column 7 of paper 3, test paper 3 is run through by card column 7 to be placed on the bottom plate 11 of deck 4；Get stuck and 5 be equipped with towards the corresponding surface of deck 4 The cutting ferrule 6 of grafting is corresponded with two card columns 7 and ring is furnished with and the matched raised line 8 for being compacted test paper 3 of bottom plate 11；Test paper 3 is adopted With quantum dot fluorescent test paper strip, formed by overlapping the sample pad 10 of connection, label pad 12, reaction film 13, water absorption pad 14 successively, sample Product pad 10 stretches out 2 suction side 9 of plastic body away from label pad 12 away from end, and is equipped with use on sample pad 10, water absorption pad 14 The circular hole (unmarked) passed through in card column 7, reaction film 13 are equipped with detection line 15 and nature controlling line 16；Get stuck 5 with 13 phase of reaction film Corresponding position is equipped with the observation window 17 for being able to observe that detection line 15 and nature controlling line 16；Deck 4 and get stuck 5 and absorption pad 14 Air hole 18 is equipped with corresponding position.Using the above scheme, the test card of the utility model has cheap, uses It is convenient, high sensitivity, quick and precisely, stability is good, personnel without other instruments and specialty, can be with Furaxone metabolite etc. Feature, as a result judge it is intuitive and reliable, suitable for the remaining on-site supervision of Furaxone metabolite in animal derived food and a large amount of samples This examination.

7 outer contour of card column in the present embodiment has the cross-sectional structure identical with 6 Internal periphery of cutting ferrule, the cross-sectional structure Using cross, one or more groups in cylindrical type, square or D fonts can also be used in various embodiments certainly Close.Preferably, card column 7 realizes tight fit with cutting ferrule 6 by way of interference fit, there is provided the structural stability of this test strips.

2 suction side of plastic body, 9 petition warhead structure in the present embodiment, can use with the type of attachment of block 1 and insert Connect, can also use and be screwed on.It can reach protection and be exposed to sample pad and the effect easy to assembling that plastic body suction side is.

Sample pad 10, label pad 12 and water absorption pad 14 deviating from face covered with glued membrane away from bottom plate 11 in the present embodiment Protective layer, to play a protective role.

The width that laminates of sample pad 10 and label pad 12 in the present embodiment is 2mm, absorption pad 14 and reaction film 13 it is folded Pressure width is 2mm, can meet use condition.

Quantum dot-labeled anti-Furaxone metabolite monoclonal antibody is perfused with label pad 12 in the present embodiment.Inspection Survey line 15 is the detection line T lines for spraying or being printed with Furaxone metabolite-carrier protein couplet thing solution on reaction film 13, Nature controlling line 16 is to spray or be printed with the nature controlling line C line of sheep anti-mouse igg solution, and detection line T lines and Quality Control on reaction film 13 Spacing between line C lines is 4mm, and detection line T lines are arranged close to the side of sample pad 10.5 are got stuck on the side of observation window 17 It is additionally provided with detection line trace " C " corresponding with detection line T lines and nature controlling line trace corresponding with nature controlling line C line " T ".

Sample pad 10 and label pad 12 in the present embodiment are hard glass fiber, and reaction film 13 is nitrocellulose filter, Bottom plate 11 is hard plastic bar or the cardboard bar not absorbed water, and water absorption pad 14 is the pure white filter made of plant long fibre is raw material Paper.

The preparation method of test card described in the utility model is：

1) preparation of anti-Furaxone metabolite monoclonal antibody-quantum dot-labeled thing：Take carboxyl water-soluble quantum dot 600 μ L, lucifuge is shaken 30 minutes after the methanol mixing of the carbodiimide hydrochloride (1mg/mL) and 800 μ L that add 18 μ L, adds 8 μ The beta -mercaptoethanol of L is terminated, and takes the anti-Furaxone metabolite monoclonal antibody (1.5mg/mL) of 10mL to add activation It is mixed with QDs, lucifuge concussion 2h, adds mercaptoethanol and stablize QDs, dialyse.In 1,6300r/min bars after dialysis Centrifuged 3 minutes under part, remove supernatant, precipitation is resuspended in 10mmol/l PBS (pH7.4), and 4 DEG C of preservations, obtain furazolidone generation Thank to thing specific antibody-quantum dot-labeled thing.

2) preparation of label pad：After hard glass tunica fibrosa is cut into strip, it is placed in containing 0.5% bovine serum albumin(BSA) (body Product percentage composition), immersion 2h in 0.1mol/L phosphate buffers (pH7.2), 37 DEG C to dry 2h spare.By anti-furazolidone metabolite Thing monoclonal antibody-quantum dot-labeled thing takes out placement to room temperature respectively, dilutes 100-1000 times into quantum with PBS-TBN respectively Point label solution, then uniformly sprays quantum dot-labeled antibody thing in hard glass fiber film surface, drying at room temperature, 4 DEG C Preserve.PBS-TBN used is sodium chloride nacl containing 20mM-150mM, 0.02%-0.1% tweens Tween-20,2mg/mL-10m/ The 10mM-100mM phosphate buffer PB of mL bovine serum albumin(BSA)s BSA, 0.05% sodium azide NaN3, obtain label pad.

3) preparation of reaction film：Nitrocellulose filter is placed on the unidirectional specking instrument platforms of X-only, furazolidone metabolite Thing-carrier protein couplet thing solution (0.5mg/ml) is put in A ponds, and sheep anti-mouse igg solution (0.5mg/ml) is put in B ponds, flattening pressure Tightly, antigen and secondary antibody are distinguished into fixed fire on nitrocellulose filter after start, forms detection line T lines and nature controlling line C line.Room temperature is certainly So after drying, 30min in confining liquid (mass concentration is the PBS buffer of 1% BSA, pH 7.4), 37 DEG C of drying are dipped in Afterwards, drier is added, 4 DEG C are sealed.

4) preparation of sample pad：Hard glass fiber be 2% containing mass concentration BSA, mass concentration be 1% sugarcane After the PBS processing for the NaN3 that the Boratex and mass concentration that sugar, mass concentration are 0.5% are 0.1%, drying for standby, is sample Product pad.

5) preparation of absorption pad：The extremely strong plant long fibre of water absorbing capacity is cut for the pure white filter paper that raw material make Machine-cut into 4mm wide slice, blend compounds film carry out one side closed, drying for standby.

6) assembling of quantum dot fluorescence test card：On bottom plate, sample pad, label pad, reaction film and absorption pad are pressed one Determine technique to fit together successively, the test strips of 3mm wide are made of CM4000 cutting machines, be packaged in test strips by certain technique In special plastic body with 1 observation window, and sample pad is stretched out in outside the plastic body suction side, be this practicality New Furaxone metabolite quantum dot fluorescence test card.The test card assembled is put in the aluminium foil bag of drier and seals Preserve.

The use of test card described in the utility model operates：

1) processing of tissue samples：The flesh of fish (kind is unlimited) or fresh water shrimp (kind is unlimited) 1 ± 0.01g are weighed in 50mL 0.1M HCl 2mL, 0.01M derivatization reagent 0.1mL are successively added in plastic centrifuge tube, with the abundant vortex mixed of vortex instrument For 1min so that tissue dispersion is uniform, constant-temperature incubation 16h or so in 37 DEG C of insulating boxs, successively adds 1M NaOH 0.2mL, acetic acid second Ester 8mL, with the abundant vortex mixed 1min of vortex instrument, 4000r/min centrifugations 5min.Supernatant 4mL is taken in 5mL plastic centrifuge tubes In, dried up in 60 DEG C or so water-bath nitrogen, be taken up in order of priority and add n-hexane 0.5mL, PBS fliud flushing 0.5mL, be vortexed after 4000r/ Min centrifuge 5min, remove clear liquid be used for analyze detection.

2) quantum dot fluorescent test paper card is taken out, by the sample pad insertion sample to be tested of test card, stops 10-15 seconds, takes Go out, observe the directly observation of result or naked eyes after 5-10 minutes in the UV lamp.

3) result judges：

It is negative：If nature controlling line C line and detection line T lines occur two at the same time on nitrocellulose filter in test card observation window During fluorescence " ‖ " trace, represent that Furaxone metabolite residual content is negative in sample solution.

It is positive：If nature controlling line C line shows fluorescence " a | " trace on nitrocellulose filter in test card observation window, and Corresponding detection line T lines do not show fluorescence, represent to be detected Furaxone metabolite residual content in sample solution and are positive.

It is invalid：If nature controlling line C line has one or two not show phosphor strip on nitrocellulose filter in test card observation window No matter band, then correspond to whether detection line T lines fluorescence trace occur, and it is invalid which is judged to.

Finally illustrate, above example is merely intended for describing the technical solutions of the present application, but not for limiting the present application, although ginseng The utility model is described in detail according to preferred embodiment, it will be understood by those of ordinary skill in the art that, can be to this The technical solution technical scheme is modified or replaced equivalently of utility model, without departing from the objective and scope of the technical program, it should all Cover among the right of the utility model.

Claims (10)

1. Furaxone metabolite quantum dot fluorescence test card, including plastic body (2), it is sheathed on plastic body suction side (9) On block (1) and the test paper (3) in plastic body, the plastic body is by deck (4) and is covered on deck Get stuck (5) composition, it is characterised in that and the deck is equipped with the card column (7) for being used for positioning test paper towards the corresponding surface both ends got stuck, Test paper is run through by card column to be placed on the bottom plate (11) of deck；The corresponding surface to get stuck towards deck is equipped with and two card columns one The cutting ferrule (6) and ring of one correspondence grafting are furnished with the raised line (8) with the matched compacting test paper of bottom plate；The test paper is quantum dot Fluorescent test paper strip, is formed, institute by overlapping the sample pad (10) of connection, label pad (12), reaction film (13), water absorption pad (14) successively State sample pad and stretch out plastic body suction side away from end away from label pad, and be equipped with sample pad, water absorption pad for card column The circular hole passed through, the reaction film are equipped with detection line (15) and nature controlling line (16)；It is described to get stuck and the corresponding position of reaction film Put equipped with the observation window (17) for being able to observe that detection line and nature controlling line；The deck and get stuck and the corresponding position of absorption pad Put and be equipped with air hole (18).

2. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that outside the card column Profile has a cross-sectional structure identical with the cutting ferrule Internal periphery, the cross-sectional structure is cylindrical type, square, D fonts or It is cross.

3. Furaxone metabolite quantum dot fluorescence test card according to claim 2, it is characterised in that the card column with Cutting ferrule tight fit.

4. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that the plastic clip Body suction side petition warhead structure, for grafting or is screwed on the type of attachment of block.

5. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that the sample Pad, label pad and water absorption pad deviate from face covered with rubber protective layer away from bottom plate.

6. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that the sample pad The width that laminates with label pad is 2mm, and the width that laminates of absorption pad and reaction film is 2mm.

7. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that the label pad On be perfused with quantum dot-labeled anti-Furaxone metabolite monoclonal antibody.

8. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that the detection line For the detection line T lines of Furaxone metabolite-carrier protein couplet thing solution, the Quality Control are sprayed or are printed with reaction film Line is the nature controlling line C line for spraying or being printed with sheep anti-mouse igg solution on reaction film, and between detection line T lines and nature controlling line C line Spacing be 4mm, detection line T lines are arranged close to the side of sample pad.

9. Furaxone metabolite quantum dot fluorescence test card according to claim 8, it is characterised in that described to get stuck Be additionally provided with the side of observation window detection line trace " C " corresponding with detection line T lines and with the corresponding Quality Control of nature controlling line C line Line trace " T ".

10. Furaxone metabolite quantum dot fluorescence test card according to claim 1, it is characterised in that sample pad and Label pad is hard glass fiber, and reaction film is nitrocellulose filter, and bottom plate is hard plastic bar or the cardboard bar not absorbed water, and is inhaled Water cushion is the pure white filter paper made of plant long fibre is raw material.