CN210690597U - Poultry egg class antibiotic remains to detect with ally oneself with card more - Google Patents
Poultry egg class antibiotic remains to detect with ally oneself with card more Download PDFInfo
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Abstract
The application belongs to the technical field of food antibiotic detection, and particularly relates to a multi-joint card for detecting residue of poultry egg antibiotics. The structure of the device comprises an external shell and test strips which are arranged in parallel in the shell in a grid manner; the external shell is of an integrated design structure or a clamping shell structure which can be opened and closed through side edges, and a plurality of independent clamping grooves are formed in the internal bottom shell through independent dividing plates; meanwhile, an observation window is arranged in the middle of each clamping groove corresponding to the face shell; each clamping groove is provided with a sample adding hole at the bottom of the corresponding face shell or provided with a sample adding groove transversely vertical to each test strip at the bottom of the face shell; the number of the clamping grooves is 3-5. The detection test strip is as follows: sulfanilamide test strip, quinolone test strip, chloramphenicol test strip, florfenicol test strip, and tetracycline test strip. The multi-link card has: after the sample is pretreated at one time, a plurality of items can be detected simultaneously, and the purposes of small volume and large flux detection can be better realized.
Description
Technical Field
The application belongs to the technical field of food antibiotic detection, and particularly relates to a multi-joint card for detecting residue of poultry egg antibiotics.
Background
Eggs are important food and nutrient sources for human beings, and antibiotics are commonly used additives for treating or preventing poultry diseases in the production process of eggs. But unqualified or illegal addition of antibiotics which are not allowed to be used or excessive addition of antibiotics makes the antibiotics which are beyond the standard easy to remain in the poultry eggs, thereby causing potential harm to human health.
Commonly used antibiotic drugs in poultry farming in the prior art include: sulfonamides, quinolones, chloramphenicol, florfenicol, tetracyclines, and the like. Because of the different properties of these drugs, the hazard to humans is different, and the regulations on whether they are allowed to be used are different, as far as the requirements of the use regulations for some specific types of antibiotics are concerned:
the sulfanilamide drugs have broad-spectrum antibacterial property and are widely used for treating bacterial infectious diseases; when the feed additive is used as a feed drug additive of livestock and poultry, if the residue is serious in animal food, the feed additive can cause anaphylactic reaction of human, influence the immune system of the organism, destroy the hematopoietic function of the human body and the like, and can cause carcinogenicity, so that the drug is limited in use by the ministry of agriculture;
quinolone is a broad-spectrum high-efficiency antibacterial drug, and is widely applied to the treatment and prevention of various infectious diseases of animals; because of large usage amount and wide application, the ministry of agriculture takes the compound as the key point for monitoring veterinary drug residues;
chloramphenicol belongs to bacteriostatic broad-spectrum antibiotics and is widely used for treating various sensitive bacteria infections, but because chloramphenicol has serious side effects, serious adverse reactions are caused to a hematopoietic system, and diseases such as human aplastic anemia and agranulocytosis can be caused, national regulations strictly prohibit the use of chloramphenicol in the animal breeding process;
florfenicol is an antibiotic drug, generates broad-spectrum antibacterial action by inhibiting the activity of peptide acyl transferase, has wide antibacterial spectrum, is widely used for treating bacterial diseases of animals, such as respiratory system infection and intestinal tract infection, and has obvious treatment effect on various bacterial diseases of livestock and poultry; however, florfenicol has certain immunotoxicity and embryotoxicity, and adverse reactions such as anorexia and diarrhea can occur after administration, so that the poultry (egg laying is forbidden) cannot be detected in poultry eggs (such as eggs) in the 235 th notice of Ministry of agriculture;
the tetracycline medicaments are broad-spectrum antibacterial agents and are widely applied to the culture of livestock, poultry and aquatic products; however, the drugs can cause various adverse reactions such as human hepatotoxicity, renal toxicity, allergic reaction and the like; and a large amount of residues in the animal body can be caused if the animals are abused for a long time in the breeding process, so that the health of consumers is seriously harmed; therefore, the method is also the focus of veterinary drug residue monitoring at present.
In the prior art, the methods for detecting the residues of the five antibiotics are mainly used for high performance liquid chromatography, liquid chromatography-mass spectrometry and the like, and the detection methods have the advantages of high sensitivity, accurate quantification, accurate qualitative determination and the like. However, these methods are expensive to detect and require specialized technicians, expensive laboratory equipment and long preliminary preparation. Thus, practical detection applications are more limited. On the other hand, in the prior art, although some products have been developed and designed for determining whether a single antibiotic has residues or not and whether the single antibiotic has the residues or not in a violation of adding, when detection and determination are required to be performed for determining whether a plurality of antibiotics have residues at one time, new detection products still need to be developed and designed, so that different use requirements are met.
Disclosure of Invention
The application aims to provide a multi-joint card product for detecting antibiotic residues of poultry eggs, so that convenience is brought to inspection and quarantine work for judging whether antibiotics are added and used illegally or whether specific antibiotics are left.
The technical solution adopted in the present application is detailed as follows.
A multi-joint card for detecting antibiotic residues in poultry eggs comprises an outer shell and detection test strips which are arranged in parallel and in a grid manner inside the shell;
the outer shell is of an integrated design structure (so as to meet the requirement of disposable use) or a clamping shell structure with the side edges capable of being opened and closed, and a plurality of independent clamping grooves (each clamping groove is equivalent to one grid) are formed in the inner bottom shell of the outer shell through independent grid plates; meanwhile, an observation window is arranged in the middle of each clamping groove corresponding to the face shell; for facilitating sample adding detection, each clamping groove is provided with a sample adding hole at the bottom of the corresponding face shell, or provided with a sample adding groove transversely vertical to each test strip at the bottom of the face shell (so as to carry out uniform sample adding on different detection test strips); in the preferred design, aiming at the sample adding slot which is transversely arranged, the water-absorbing filter paper is designed in the sample adding slot, so that a sample to be added can be uniformly and quickly dispersed to the sample adding parts corresponding to different test strips;
in the preferred design, a cover plate made of transparent material (for example, a cover plate made of acrylic material) is designed on the face shell corresponding to the observation window arranged on each clamping groove, so that the detection result is not influenced by the external environment;
the specific preparation material of the outer shell can adopt a PVC material;
the number of the card slots is specifically 3, 4 or 5, for example, and a three-link card, a four-link card or a five-link card product can be correspondingly formed; however, it should be noted that the number of the slots is not too large, and the main reasons are as follows: when the quantity is excessive, the size of the product specification is too large, the use is not convenient enough, and the detection cost is easy to increase; on the other hand, when the number of the clamping grooves is too large, certain influence is easily formed on the sample loading uniformity.
Taking the number of the card slots as 5 (corresponding to a quintuplet card product) as an example, the specification of the whole external shell can be designed as follows: the length is 70mm and the width is 65 mm.
The detection test strip can specifically adopt one or any combination of the following detection test strips (when the same detection test strip is adopted, the detection can be carried out on different samples, and when the same detection test strip is adopted, different detection test strips are used as controls to ensure the repeatability of a detection result, and when the different detection test strips are adopted, the same detection can be carried out on whether the same sample contains different antibiotic residues or not, and whether different types of antibiotics have residues or not can be carried out on different samples):
the specific detection test strip type comprises a sulfonamide detection test strip, a quinolone detection test strip, a chloramphenicol detection test strip, a florfenicol detection test strip and a tetracycline detection test strip, and the specific detection test strip structure is as follows:
the device comprises a bottom plate, a display part arranged in the middle of the bottom plate, and a sample adding part and a power part arranged at two ends of the bottom plate, wherein a reaction part is arranged between the sample adding part and the display part;
the sample adding part and the reaction part, the reaction part and the display part, and the display part and the power part are tightly connected; the tight connection can be realized by adopting a partial overlapping mode (the length of the overlapped part is about 1-2 mm) to ensure tight connection;
the bottom plate is mainly used for supporting all structural components, does not participate in reaction, and has certain hardness and corrosion resistance in view of the characteristics of partial chemical materials, and the preparation material is specifically, for example, a PVC material;
the display part is specifically a nitrocellulose membrane provided with a detection line and a quality control line;
the sample adding part is a sample pad prepared by adopting glass cellulose with a certain adsorption function; in the preferred design, the sample pad is prepared by adopting a glass cellulose membrane after being treated by buffer solution; the buffer is specifically, for example, a PB (pH = 8.0) buffer (phosphate buffer) at a concentration of 0.05M; the buffer solution has better ionic strength and more proper pH value, and can play a better buffering role on a detected sample, thereby improving the sample loading performance and shielding the interference of impurities;
the power part is a structural part made of a material with a liquid flow absorption function, and is specifically made of absorbent filter paper with strong water absorption capacity; for example, the water absorption capacity of the water absorption paper with the specification of 370 +/-10 grams per square meter can reach 750 +/-100 grams per square meter, so that the flow speed of related liquid can be better met, and the detection result can be conveniently and quickly judged;
the reaction part is a gold-labeled antibody combination pad prepared by a glass cellulose membrane coated with colloidal gold and an antibody combination; in the marking process, the antibody concentration and the marking amount of the gold-labeled antibody (colloidal gold and an antibody conjugate) are different (different dosage mainly ensures the reaction effect of the final test strip) aiming at different types of test strips:
aiming at the test strip for detecting sulfonamides, the concentration of the sulfonamides antibody is 9.6mg/ml, and the amount of the gold-labeled antibody is 0.8 mul/ml;
aiming at the quinolone detection test strip, the concentration of the quinolone antibody is 10.2mg/ml, and the labeling amount of the gold-labeled antibody is 0.6 mu l/ml;
for a chloramphenicol detection test strip, the concentration of the chloramphenicol antibody is 3.35mg/ml, and the amount of the gold-labeled antibody is 1.2 μ l/ml;
aiming at the florfenicol detection test strip, the concentration of a florfenicol antibody is 7.1mg/ml, and the labeling amount of a gold-labeled antibody is 0.75 mu l/ml;
aiming at the tetracycline detection test strip, the concentration of the tetracycline antibody is 7.1mg/ml, and the labeling amount of the gold-labeled antibody is 1.2 mul/ml.
It should be noted that the quality control line scribed on the nitrocellulose membrane is designed near the power part (absorbent paper end), and the detection line is arranged near the sample addition part (sample pad end); the distance between the detection line and the quality control line can be set to be 5 mm; in the specific preparation, aiming at different types of test strips, the amounts of the detected substance-carrier protein conjugate and the rabbit anti-mouse IgG used when the display part is marked with a detection line (T line) and a quality control line (C line) are slightly different, and the following can be referred to:
aiming at the sulfanilamide test strip, the concentration of the detected substance-carrier protein conjugate is 11.6mg/ml, and the scribing quantity of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 10;
aiming at the quinolone detection test strip, the concentration of the detected substance-carrier protein conjugate is 16mg/ml, and the scribing amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T3.5;
for a chloramphenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 5.6mg/ml, and the cross-line amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 8;
aiming at the florfenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 15.3mg/ml, and the line drawing amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C22T 3;
aiming at the tetracycline detection test strip, the concentration of the test object-carrier protein conjugate is 15mg/ml, and the cross-line quantity of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 4.
Furthermore, the nitrocellulose membrane is specifically an mdi CNPC-SS24 type nitrocellulose membrane product, the size of the pore diameter of the nitrocellulose membrane is 10 μm, the nitrocellulose membrane has good water running performance, and has the advantages of good sensitivity, strong aging resistance, small batch difference and the like, and the nitrocellulose membrane can better meet the use requirements;
the glass cellulose membrane is a product with the Huaiyuan K-75 specification, has the advantages of uniform and compact lines, good hydrophilicity, strong water storage capacity and the like, and can better ensure the quality of a final detection strip.
In a specific finished product, the specific specification of the test strip is, for example: the length is 60mm and the width is 4.00 mm.
In the existing detection technology, the detection method based on the recognition of specific molecules of antibodies to antigens in immunology has the advantages of high affinity, rapidness, simplicity, economy, practicality and the like, and can realize the detection with small volume and large flux, so the method is one of the development directions of the detection technology of antibiotic drug residues. On the other hand, in the existing detection of the poultry egg antibiotics, the number of samples to be detected is large due to the variety of the samples to be detected. Based on the development trend and the actual detection requirement of the detection technology, the inventor develops and designs a multi-joint card detection product for detecting whether the antibiotic residues exist in the poultry egg products.
In general, the multi-gang card provided by the application has the following technical advantages:
1. according to the needs, the triple, quadruple or quintuplet structure of different forms can be adopted, when specifically using: the method has the advantages that the method only needs one sample pretreatment process, but can simultaneously detect the residual conditions of various avian egg antibiotics, so that the operation is efficient and quick, and the method is suitable for preliminary screening detection of various and large amount of samples;
2. the sensitivity is high, and the specificity is strong; the detection card is prepared by labeling a high-affinity monoclonal antibody with colloidal gold, gold particles in the gold-labeled antibody are combined with antibody molecules through the van der Waals force of opposite charges, and the colloidal gold has little influence on the specificity and the affinity of the monoclonal antibody; the detection limit of each variety can reach respectively: 20-50ppb of sulfanilamide, 20-50ppb of quinolone, 10ppb of chloramphenicol, 30ppb of florfenicol, and 20-50ppb of tetracycline;
3. the treated sample solution is dripped into the sample adding hole of the test paper card by a dropper for 4-6 drops (when dripping respectively, if adding samples uniformly, the sample adding amount should be increased properly), and the result can be observed within 4-6min without any other reagent;
4. the result interpretation is simple and intuitive: two purple red bands appear, which indicates that the sample does not contain the detected object or the concentration of the detected object is lower than the detection limit; when the detection T line is colorless or nearly colorless, the concentration of the detected object in the sample is equal to or higher than the detection limit; if the line C does not appear, the operation process is incorrect or the detection card fails, and the detection strip needs to be replaced again or the detection card needs to be detected again;
5. use the utility model discloses the test paper card need not join in marriage complicated instrument and equipment and expensive reagent in addition, and the witnessed inspections targets in place in one step, low cost, and it is fast to take effect, consequently easily popularizes and applies on a large scale.
Drawings
FIG. 1 is a schematic external view of an external housing of a multi-gang card;
FIG. 2 is a schematic diagram of an internal test strip structure;
FIG. 3 is a reference image for interpretation of the results of the detection;
in the figure: 1. a base plate; 2. a sample addition part; 3. a reaction section; 4. a display unit; 5. detecting lines; 6. a quality control line; 7. a power section; 8. a sample adding hole 9 and an observation window.
Detailed Description
The present application is further illustrated by the following examples. Before introducing specific examples, it should be noted that rabbit anti-mouse IgG, anti-test substance monoclonal antibody, test substance-carrier protein conjugate, etc. involved in the following examples are all commercially available or conventionally prepared according to the prior art, and are not described again.
Examples
As shown in fig. 1, the poultry egg antibiotic residue detection multi-gang card provided by the application comprises an outer shell and a detection test strip arranged in parallel and in a grid manner inside the shell (the test strip structure is shown in fig. 2);
the outer shell is of an integrated design structure (so as to meet the requirement of disposable use) or a clamping shell structure which can be opened and closed through side edges, and a plurality of independent clamping grooves (each clamping groove is equivalent to a grid) (not shown in the figure) are formed in the inner bottom shell through independent grid plates; meanwhile, an observation window 9 is arranged in the middle of each clamping groove corresponding to the face shell; for facilitating sample adding detection, each clamping groove is provided with a sample adding hole 8 at the bottom of the corresponding face shell, or provided with a sample adding groove transversely vertical to each test strip at the bottom of the face shell (so as to carry out uniform sample adding on different detection test strips); in the preferred design, to the application of sample groove of horizontal setting, be designed with absorbent filter paper in the application of sample groove to ensure that the sample that adds can be more for even and quick dispersion to the application of sample position that different test paper strips correspond (shown in the figure only be independent application of sample hole design, in the middle of the actual design, can directly design for horizontal application of sample groove, cooperate the absorbent filter paper of application of sample groove department design simultaneously, thereby can once the application of sample, detect simultaneously).
In the preferred design, a cover plate made of transparent material (for example, a cover plate made of acrylic material) is designed on the face shell corresponding to the observation window arranged on each clamping groove, so that the detection result is not influenced by the external environment;
the external shell is made of PVC material.
The number of the card slots is specifically 3, 4 or 5, for example, and a three-link card, a four-link card or a five-link card product can be correspondingly formed; however, it should be noted that the number of the slots is not too large, and the main reasons are as follows: when the quantity is excessive, the size of the product specification is too large, the use is not convenient enough, and the detection cost is easy to increase; on the other hand, when the number of the clamping grooves is too large, certain influence is easily formed on the sample loading uniformity.
Taking the number of the card slots as 5 (corresponding to a quintuplet card product, namely shown in the figure) as an example, the specification of the whole external shell can be designed as follows: the length is 70mm and the width is 65 mm.
The detection test strip can specifically adopt one or any combination of the following detection test strips (when the same detection test strip is adopted, the detection can be carried out on different samples, and when the same detection test strip is adopted, different detection test strips are used as controls to ensure the repeatability of a detection result, and when the different detection test strips are adopted, the same detection can be carried out on whether the same sample contains different antibiotic residues or not, and whether different types of antibiotics have residues or not can be carried out on different samples):
the specific detection test strip type comprises a sulfonamide detection test strip, a quinolone detection test strip, a chloramphenicol detection test strip, a florfenicol detection test strip and a tetracycline detection test strip, and the specific detection test strip structure is shown in figure 2 and specifically comprises the following steps:
the device comprises a bottom plate 1, a display part 4 arranged in the middle of the bottom plate 1, a sample adding part 2 and a power part 7 arranged at two ends of the bottom plate 1, wherein a reaction part 3 is arranged between the sample adding part 2 and the display part 4;
the sample adding part 2 is tightly connected with the reaction part 3, the reaction part 3 is tightly connected with the display part 4, and the display part 4 is tightly connected with the power part 7; the tight connection can be realized by adopting a partial overlapping mode (the length of the overlapped part is about 1-2 mm) to ensure tight connection.
The bottom plate 1 is mainly used for supporting all structural components, does not participate in reaction, and has certain hardness and corrosion resistance in view of the characteristics of partial chemical materials, and the preparation material is specifically, for example, a PVC material;
the display part 4 is specifically a nitrocellulose membrane provided with a detection line 5 and a quality control line 6;
the sample adding part 2 is a sample pad prepared by adopting glass cellulose with a certain adsorption function; in the preferred design, the sample pad is prepared by adopting a glass cellulose membrane after being treated by buffer solution; the buffer is specifically, for example, a PB (pH = 8.0) buffer (phosphate buffer) at a concentration of 0.05M; the buffer solution has better ionic strength and more proper pH value, and can play a better buffering role on a detected sample, thereby improving the sample loading performance and shielding the interference of impurities;
the power part 7 is a structural part made of a material with a function of absorbing liquid flow, and is specifically made of absorbent filter paper with strong water absorption capacity; more specifically, for example, the water absorption capacity of the water absorption paper with the specification of 370 +/-10 grams per square meter can reach 750 +/-100 grams per square meter, so that the flow speed of related liquid can be better met, and the detection result can be conveniently and quickly judged.
The reaction part 3 is a gold-labeled antibody combination pad prepared by a glass cellulose membrane coated with colloidal gold and an antibody combination substance; in the marking process, the antibody concentration and the marking amount of the gold-labeled antibody (colloidal gold and an antibody conjugate) are different (different dosage mainly ensures the reaction effect of the final test strip) aiming at different types of test strips:
aiming at the test strip for detecting sulfonamides, the concentration of the sulfonamides antibody is 9.6mg/ml, and the amount of the gold-labeled antibody is 0.8 mul/ml;
aiming at the quinolone detection test strip, the concentration of the quinolone antibody is 10.2mg/ml, and the labeling amount of the gold-labeled antibody is 0.6 mu l/ml;
for a chloramphenicol detection test strip, the concentration of the chloramphenicol antibody is 3.35mg/ml, and the amount of the gold-labeled antibody is 1.2 μ l/ml;
aiming at the florfenicol detection test strip, the concentration of a florfenicol antibody is 7.1mg/ml, and the labeling amount of a gold-labeled antibody is 0.75 mu l/ml;
aiming at the tetracycline detection test strip, the concentration of the tetracycline antibody is 7.1mg/ml, and the labeling amount of the gold-labeled antibody is 1.2 mul/ml.
In the specific design, a quality control line 6 marked on the nitrocellulose membrane is designed near a power part 7 (a water absorption paper end), and a detection line 5 is arranged near a sample adding part 2 (a sample pad end); the distance between the detection line and the quality control line can be set to be 5 mm; in the specific preparation, aiming at different types of test strips, the amounts of the detected substance-carrier protein conjugate and the rabbit anti-mouse IgG used when the display part is marked with a detection line (T line) and a quality control line (C line) are slightly different, and the following can be referred to:
aiming at the sulfanilamide test strip, the concentration of the detected substance-carrier protein conjugate is 11.6mg/ml, and the scribing quantity of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 10;
aiming at the quinolone detection test strip, the concentration of the detected substance-carrier protein conjugate is 16mg/ml, and the scribing amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T3.5;
for a chloramphenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 5.6mg/ml, and the cross-line amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 8;
aiming at the florfenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 15.3mg/ml, and the line drawing amount of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C22T 3;
aiming at the tetracycline detection test strip, the concentration of the test object-carrier protein conjugate is 15mg/ml, and the cross-line quantity of rabbit anti-mouse IgG (the concentration is 8 mg/ml) is C25T 4.
Furthermore, the nitrocellulose membrane is specifically an mdi CNPC-SS24 type nitrocellulose membrane product, the size of the pore diameter of the nitrocellulose membrane is 10 μm, the nitrocellulose membrane has good water running performance, and has the advantages of good sensitivity, strong aging resistance, small batch difference and the like, and the nitrocellulose membrane can better meet the use requirements;
the glass cellulose membrane is a product with the Huaiyuan K-75 specification, has the advantages of uniform and compact lines, good hydrophilicity, strong water storage capacity and the like, and can better ensure the quality of a final detection strip.
In a specific finished product, the specific specification of the test strip is, for example: the length is 60mm and the width is 4.00 mm.
When the multi-link card is used for detecting and judging whether the poultry egg sample contains prohibited antibiotics or whether antibiotics remain, the poultry egg sample to be detected needs to be pretreated to obtain a liquid to be detected, and the specific sample pretreatment mode can refer to the following steps:
taking 1ml of fully and uniformly stirred fresh egg liquid (such as eggs) by using a 1ml straw, adding 5ml of pure water into a 10ml centrifuge tube, tightly covering the centrifuge tube cover, shaking up and down by hand, uniformly mixing for 1min, and sampling for detection.
When the detection reagent is specifically applied, the test paper card is flatly placed, the liquid to be detected is dripped into the sample adding hole by using the dropper, 4-6 drops are dripped, and the result is observed after 4-6min (if the structural design of the sample adding groove is adopted, the sample adding amount is properly increased, for example, at least 30 drops are increased, so as to ensure that enough samples are arranged at each detection test paper strip position).
The specific result judgment reference is shown in fig. 3:
negative (-): two purple red bands appear, which indicates that the sample does not contain the detected object or the concentration of the detected object is lower than the detection limit;
positive (+): when the detection T line is colorless or nearly colorless, the concentration of the detected object in the sample is equal to or higher than the detection limit; and (4) invalidation: the absence of line C (two right-most panels in fig. 3) indicates an incorrect operation or a failed test card, requiring replacement of the test strip or retesting.
In general, the rapid poultry egg detection multi-link card comprises: the method has the advantages of simultaneously detecting a plurality of items after pretreating the sample at one time, well realizing the detection purposes of small volume and large flux, and being suitable for various production detection scenes such as laboratory detection, customs quarantine, health supervision, scale culture, individual production and the like due to the advantages of convenient operation, quick and accurate detection result, high sensitivity, low cost, good stability and the like, and can lay a good detection technical foundation for ensuring the safety and health of food.
Claims (8)
1. A multi-joint card for detecting antibiotic residues in poultry eggs is characterized by comprising an outer shell and detection test strips which are arranged in parallel and in a grid manner inside the shell;
the external shell is of an integrated design structure or a clamping shell structure which can be opened and closed through side edges, and a plurality of independent clamping grooves are formed in the internal bottom shell through independent dividing plates; meanwhile, an observation window is arranged in the middle of each clamping groove corresponding to the face shell; a sample adding groove transversely vertical to each test strip is arranged at the bottom of the face shell; meanwhile, water absorption filter paper is designed in the sample adding groove;
the number of the clamping grooves is 3, 4 or 5, and three-link cards, four-link cards or five-link card products can be correspondingly formed.
2. The multi-connected card for detecting antibiotic residues in poultry eggs as claimed in claim 1, wherein the face shell is provided with a cover plate made of transparent material at a position corresponding to the observation window of each card slot.
3. The poultry egg antibiotic residue detection multi-joint card as claimed in claim 1, wherein the outer shell is made of PVC.
4. The poultry egg antibiotic residue detection multi-connected card as claimed in claim 1, wherein when the number of the card slots is 5, the specification of the whole outer shell is designed as follows: the length is 70mm and the width is 65 mm.
5. The multiplex card for detecting avian egg antibiotic residues as claimed in claim 1, wherein the detection test strip specifically adopts one or any combination of the following detection test strips: a sulfanilamide detection test strip, a quinolone detection test strip, a chloramphenicol detection test strip, a florfenicol detection test strip, and a tetracycline detection test strip; the structure of the detection test strip is as follows:
the device comprises a bottom plate, a display part arranged in the middle of the bottom plate, and a sample adding part and a power part arranged at two ends of the bottom plate, wherein a reaction part is arranged between the sample adding part and the display part;
the sample adding part and the reaction part, the reaction part and the display part, and the display part and the power part are tightly connected;
the display part is specifically a nitrocellulose membrane provided with a detection line and a quality control line;
the sample adding part is a sample pad prepared by adopting glass cellulose with an adsorption function;
the power part is a structural part made of a material with a liquid flow adsorption function, and is specifically made of absorbent filter paper;
the reaction part is a gold-labeled antibody combination pad prepared by a glass cellulose membrane coated with colloidal gold and an antibody combination; in the marking process, aiming at different types of test strips, the antibody concentration and the gold-labeled antibody marking amount are different:
aiming at the test strip for detecting sulfonamides, the concentration of the sulfonamides antibody is 9.6mg/ml, and the amount of the gold-labeled antibody is 0.8 mul/ml;
aiming at the quinolone detection test strip, the concentration of the quinolone antibody is 10.2mg/ml, and the labeling amount of the gold-labeled antibody is 0.6 mu l/ml;
for a chloramphenicol detection test strip, the concentration of the chloramphenicol antibody is 3.35mg/ml, and the amount of the gold-labeled antibody is 1.2 μ l/ml;
aiming at the florfenicol detection test strip, the concentration of a florfenicol antibody is 7.1mg/ml, and the labeling amount of a gold-labeled antibody is 0.75 mu l/ml;
aiming at the tetracycline detection test strip, the concentration of the tetracycline antibody is 7.1mg/ml, and the labeling amount of the gold-labeled antibody is 1.2 mul/ml.
6. The multiplex card for detecting avian egg antibiotic residues as claimed in claim 5, wherein in the detection strip, the quality control line scribed on the nitrocellulose membrane is arranged near the dynamic part, and the detection line is arranged near the sample addition part; during specific preparation, aiming at different types of test strips, the dosage of a detected substance-carrier protein conjugate and the dosage of rabbit anti-mouse IgG adopted when a detection line and a quality control line are marked on a display part are slightly different, and the specific steps are as follows:
aiming at the sulfanilamide test strip, the concentration of the detected substance-carrier protein conjugate is 11.6mg/ml, and the scribing amount of rabbit anti-mouse IgG is C25T 10;
aiming at the quinolone detection test strip, the concentration of the detected substance-carrier protein conjugate is 16mg/ml, and the line drawing amount of rabbit anti-mouse IgG is C25T3.5;
for a chloramphenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 5.6mg/ml, and the amount of the rabbit anti-mouse IgG is C25T 8;
aiming at the florfenicol detection test strip, the concentration of the detected substance-carrier protein conjugate is 15.3mg/ml, and the cross-line quantity of rabbit anti-mouse IgG is C22T 3;
aiming at the tetracycline detection test strip, the concentration of the detected substance-carrier protein conjugate is 15mg/ml, and the line drawing amount of rabbit anti-mouse IgG is C25T 4.
7. The multiplex card for detecting avian egg antibiotic residues as claimed in claim 5, wherein in the detection test strip, the nitrocellulose membrane is a mdi CNPC-SS24 type nitrocellulose membrane product;
the glass cellulose membrane is a product with the Huaiyuan K-75 specification.
8. The multiplex card for detecting avian egg antibiotic residues as claimed in claim 5, wherein the detection test strip has the specific specifications: the length is 60mm and the width is 4.00 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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