CN205982288U - A box is hatched to antibody for immunoblotting experiment - Google Patents
A box is hatched to antibody for immunoblotting experiment Download PDFInfo
- Publication number
- CN205982288U CN205982288U CN201620864068.9U CN201620864068U CN205982288U CN 205982288 U CN205982288 U CN 205982288U CN 201620864068 U CN201620864068 U CN 201620864068U CN 205982288 U CN205982288 U CN 205982288U
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- valve bag
- liquid outlet
- antibody
- filling opening
- bag
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Abstract
The utility model belongs to the technical field of biological assay, a a box is hatched to antibody for immunoblotting experiment is related to, including valve bag, last liquid outlet, the filling opening of setting up of valve bag, the liquid outlet highly be higher than the filling opening, liquid outlet and filling opening set up a three -way valve respectively, it is big young coniform in, that the tubulose interface of three -way valve is the collar extension, and valve bag is last to be formed a stereoplasm and hatch the cavity, the product of the utility model is simple in structure, design benefit is reasonable, can effectively practice thrift antibody in the use, saves reagent, can effectively avoid the production of bubble, the device can recycle very environmental protection through abundant washing after the use simultaneously, in addition, the transparent hard tube that is connected of valve bag can be used for observing valve bag and whether add the hydroful.
Description
Technical field
This utility model belongs to field of bioexperiment technology, is related to a kind of antibody incubation box for immunoblot experiment.
Background technology
Antibody is reacted with the albumen on NC or pvdf membrane.Antibody dilution is dissolved in 5%TBST milk or antibody dilution
In liquid.In testing at present, Normal practice is the film band turning purposeful albumen to be placed in antibody incubation box, then in box
Corresponding grid puts into corresponding film and antibody diluent.The shortcoming of such way is that antibody usage amount is big.Every lattice are general
Need 10 milliliters to 20 milliliters of antibody diluent.Used in experiment, antibody is very expensive, and especially one resists.So behaviour
In work, researcher to save antibody often through the usage amount reusing antibody diluent and minimizing antibody diluent.At certain
A little laboratorys, researcher, by using plastic sheeting, according to the stripe size shearing off, is sealed out almost big with film with plastic packaging machine
Little sack, adds the antibody diluent of 2-5 milliliter in sack.But the defect of such method is to be required for weight every time
Newly make sack, time-consuming, not environmentally.Made the sack coming to be easy to produce bubble during sample-adding simultaneously.In plastic bag,
If the place occupied by bubble is in the top of film, just it is easy to the reaction of meeting blocking antibody and albumen on film.This shortcoming institute
The result leading to is in the place not reacted caused by bubble, and display band would not in the later stage, may cause
False negative result, leads to the failure of an experiment.
Content of the invention
This utility model is directed to the problems referred to above, provides a kind of antibody incubation box for immunoblot experiment, this incubation box
Antibody can effectively be saved, save reagent, avoid the generation of above-mentioned bubble simultaneously.
According to the technical solution of the utility model：A kind of antibody incubation box for immunoblot experiment it is characterised in that：
Including valve bag, setting liquid outlet, filling opening on described valve bag, the height of liquid outlet is higher than filling opening, liquid outlet and liquid feeding
Mouth is respectively provided with a three-way valve, and the tube-like interface of described three-way valve is in coniform, the formation on valve bag that collar extension is big, internal orifice is little
One hard is incubated cavity.
As further improvement of the utility model, the longitudinal cross-section of described valve bag is rectangle shape, and valve bag is at least
Two sides arranges self-sealed article, and described liquid outlet and filling opening are arranged on the same sealing side of valve bag.
As further improvement of the utility model, the volume of described valve bag is 2 ~ 5 milliliters, and the height of incubation cavity is
4 ~ 9 millimeters.
As further improvement of the utility model, described liquid outlet is connected on valve bag by transparent hard tube.
As further improvement of the utility model, described valve bag outer surface arranges label bag.
Of the present utility model have technical effect that：This utility model product structure is simple, design is ingenious rationally, is using
The effectively save antibody of energy in journey, saves reagent, can be prevented effectively from the generation of bubble；This device is after a procedure through abundant water simultaneously
Wash and can be recycled, very environmental protection；In addition, the transparent hard tube that valve bag is connected can be used for observing valve bag whether liquid feeding
Full.
Brief description
Fig. 1 is structural representation of the present utility model.
Specific embodiment
Below in conjunction with the accompanying drawings specific embodiment of the present utility model is further described.
In Fig. 1, including valve bag 1, liquid outlet 2, filling opening 3, incubation cavity 4, three-way valve 5, transparent hard tube 6, label bag 7
Deng.
As shown in figure 1, this utility model is a kind of antibody incubation box for immunoblot experiment, including valve bag 1, institute
State setting liquid outlet 2, filling opening 3 on valve bag 1, the height of liquid outlet 2 is higher than filling opening 3, liquid outlet 2 is with filling opening 3 respectively
One three-way valve 5 of setting, the tube-like interface of described three-way valve 5 is in coniform, the formation one on valve bag 1 that collar extension is big, internal orifice is little
Individual hard is incubated cavity 4.
The longitudinal cross-section of valve bag 1 is rectangle shape, valve bag 1 at least two sides setting self-sealed article, described liquid outlet 2 with plus
Liquid mouth 3 is arranged on the same sealing side of valve bag 1.
The volume of valve bag 1 is 2 ~ 5 milliliters, and the height of incubation cavity 4 is 4 ~ 9 millimeters.
Liquid outlet 2 is connected on valve bag 1 by transparent hard tube 6, and transparent hard tube 6 is convenient to observe liquid feeding situation.
Valve bag 1 outer surface arranges label bag 7, is used for placing the mark of title and the parameters of experimental film etc. in label bag
Sign.
Work process of the present utility model is as follows：This utility model product, when using, is first turned on valve bag 1, film
After putting into incubation cavity 4, then valve bag 1 open side is sealed, open liquid outlet 2 and filling opening 3, and by valve bag 1 somewhat
Tilt, make liquid outlet 2 position higher,（Using gravity, so that bubble is up walked, gone out by liquid outlet）.Even if there being a small amount of residual
Bubble, somewhat beat just as gravity and bubble is driven away.So there will be neither bubble, can be film and antibody diluent again
Combine well and react.Whole valve bag 1 cumulative volume controls in 2-5 milliliter.Because the film containing destination protein is very thin, institute
As long as just permissible at several millimeters with the height of incubation cavity 4.
Because the destination protein that different researchers is studied is different, the film size cut is all different.Due to originally setting
The antibody incubation box for immunoblot experiment of meter is also fairly simple, in the Process Design making and can manufacture different size
Incubation box.One laboratory, according to common size, is bought some different size of sacks and just can be met most experiments.
This antibody incubation bag can recycle after sufficiently cleaning simultaneously, so very environmental protection.This incubation box is easy to use, effectively
Avoid bubble, greatly save antibody consumption, saved the experiment fees of preciousness.
Filling opening 3 and liquid outlet 2 use three-way valve 5, and it is coniform that the tubular inlet of three-way valve is in that collar extension is big, internal orifice is little,
Agree with 1 milliliter used in experimental implementation of pipette tips phase.And inner face uses frosted structure, effectively prevent sending out of leakage
Raw.
Claims (5)
1. a kind of antibody incubation box for immunoblot experiment it is characterised in that：Including valve bag（1）, described valve bag（1）
Upper setting liquid outlet（2）, filling opening（3）, liquid outlet（2）Height be higher than filling opening（3）, liquid outlet（2）With filling opening（3）Point
She Zhi not a three-way valve（5）, described three-way valve（5）Tube-like interface be in collar extension is big, internal orifice is little coniform, valve bag（1）
Upper one hard of formation is incubated cavity（4）.
2. be used for as claimed in claim 1 the antibody incubation box of immunoblot experiment it is characterised in that：Described valve bag（1）
Longitudinal cross-section be rectangle shape, valve bag（1）At least two sides setting self-sealed article, described liquid outlet（2）With filling opening（3）Setting
In valve bag（1）Same sealing side on.
3. be used for as claimed in claim 1 the antibody incubation box of immunoblot experiment it is characterised in that：Described valve bag（1）
Volume be 2 ~ 5 milliliters, be incubated cavity（4）Height be 4 ~ 9 millimeters.
4. be used for as claimed in claim 1 the antibody incubation box of immunoblot experiment it is characterised in that：Described liquid outlet（2）
By transparent hard tube（6）It is connected to valve bag（1）On.
5. be used for as claimed in claim 1 the antibody incubation box of immunoblot experiment it is characterised in that：Described valve bag（1）
Outer surface arranges label bag（7）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620864068.9U CN205982288U (en) | 2016-08-10 | 2016-08-10 | A box is hatched to antibody for immunoblotting experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620864068.9U CN205982288U (en) | 2016-08-10 | 2016-08-10 | A box is hatched to antibody for immunoblotting experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205982288U true CN205982288U (en) | 2017-02-22 |
Family
ID=59977183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620864068.9U Expired - Fee Related CN205982288U (en) | 2016-08-10 | 2016-08-10 | A box is hatched to antibody for immunoblotting experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205982288U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109254160A (en) * | 2018-11-09 | 2019-01-22 | 上海交通大学医学院附属第九人民医院 | A kind of automatic Western Blot antibody incubation box |
-
2016
- 2016-08-10 CN CN201620864068.9U patent/CN205982288U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109254160A (en) * | 2018-11-09 | 2019-01-22 | 上海交通大学医学院附属第九人民医院 | A kind of automatic Western Blot antibody incubation box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170222 Termination date: 20170810 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |