CN205710766U - A kind of Tissue Culture Plate preventing edge effect - Google Patents
A kind of Tissue Culture Plate preventing edge effect Download PDFInfo
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- CN205710766U CN205710766U CN201620220909.2U CN201620220909U CN205710766U CN 205710766 U CN205710766 U CN 205710766U CN 201620220909 U CN201620220909 U CN 201620220909U CN 205710766 U CN205710766 U CN 205710766U
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- reservoir
- cell culture
- tissue culture
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- culture plate
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Abstract
This utility model relates to a kind of Tissue Culture Plate preventing edge effect, and its structure is on the plate body of described Tissue Culture Plate, arranges the reservoir around outer rim cell culture well.Tissue Culture Plate described in the utility model; the sterile liquid such as PBS or sterilized water, during cell is cultivated, the liquid in preferential evaporation reservoir is added in reservoir; cell culture fluid in the cell culture well at edge can be played a protective role, edge effect will not be caused.
Description
Technical field
This product relates to cell culture system technical field, the edge that the most pre-vaporization prevention causes
The Tissue Culture Plate of effect.
Background technology
Tissue Culture Plate is experiment consumptive material conventional in cell experiment, is especially used for relating to cell
In the experiment of growth activity, such as MTT experiment, XTT experiment, CCK-8 experiment etc..Due to
The multiplex 96 porocyte culture plates of upper experiment, illustrate as a example by 96 orifice plates so main, these
Experiment is required to the strictest comparison, and in each hole, the amount of liquid must be identical, if it is not the same,
When carrying out microplate reader detection, the result obtained will be affected, and due to amount of liquid not
With, cell grows also disunity, without comparability.The total amount of liquid in its each hole required is equal,
Be not only only initial incubation time every hole total amount of liquid equal, and include in whole experimentation
It is all such.But current Tissue Culture Plate is tested, every when guarantee that can only be artificial is initial
The amount of liquid in one hole is equal, when putting into (usually 48h) after incubator cultivates a period of time, and training
Supporting will be the fewest the closer to amount of liquid in the culture hole at edge on plate, thus produces experimental error.
At present, also there is the method tackling this problem, that is, by one week of Tissue Culture Plate edge
Hole is available, and fills with the sterile liquid such as PBS or distilled water, and remaining hole is just tested.But
Being so to waste very much Tissue Culture Plate, such as 96 orifice plates, the hole of a week, edge has 36, and 36
Individual Kong Jun does not tests, and is used for doing 60 the most internal holes tested, wastes 37.5%.
And edge hole is when adding PBS, adding of need one hole, hole one, complex operation, particularly this type of reality
Test to generally require and do several blocks of plates simultaneously, easily make mistakes, sometimes due to added liquid, easily shake
Go out, and enter the experimental port closed on.
Utility model content
(1) to solve the technical problem that
Steam by antermarginal culture hole liquid for what Tissue Culture Plate of the prior art existed
Sending out speed fast, affect the defect of experimental result, this utility model provides one that edge can be prevented to imitate
The Tissue Culture Plate answered.
(2) technical scheme
Tissue Culture Plate described in the utility model, compared with traditional Tissue Culture Plate, its district
It is not, on the plate body of described Tissue Culture Plate, the storage around outer rim cell culture well is set
Liquid bath.The sterile liquid such as PBS or sterilized water is added, in the process that cell is cultivated in reservoir
In, the liquid in preferential evaporation reservoir, the cell in the cell culture well at edge can be cultivated
Liquid plays a protective role, and will not cause edge effect.
The width of described reservoir and the diameter ratio of cell culture well are 0.8～1.2.Reservoir
Width is similar with the diameter of cell culture well, correspondingly disengagement area and the evaporation of cell culture well
Area is similar, and therefore its evaporation rate is close with the evaporation rate of the cell culture well at edge, can
The cell culture well at edge is played and protects to greatest extent.
The degree of depth of described reservoir is 0.8～1.2 with the ratio of the degree of depth of described cell culture well.Liquid storage
The degree of depth of groove is similar with the degree of depth of cell culture well, can analog cell culture hole, storage fully
Appropriate liquid.
The upper surface of described reservoir and the upper surface flush of described cell culture well, cover plate can cover
Upper surface in described reservoir.
Described reservoir is 1～5mm with the distance of described plate body outer rim.This distance both can ensure
In reservoir, liquid fully evaporates and the liquid in the cell culture well at edge is played protection
Effect, also will not because of with plate body inwall apart from excessive and bring waste of material.
Described reservoir is 3～6mm with the distance of described outer rim cell culture well.In experimental implementation
During, due to the movement of Tissue Culture Plate, easily cause the liquid in culture tank and enter thin
Born of the same parents' culture hole, the distance of 3～6mm both can avoid liquid in culture tank to enter cell culture well,
Also do not result in the waste in Tissue Culture Plate space.
The corner of described reservoir is fillet.The design of fillet is more beneficial for liquid in reservoir
Science distribution, prevent the generation of edge effect.
Preferably, on the plate body of described Tissue Culture Plate, arrange around outer rim cell culture well
Reservoir；The width of described reservoir and the diameter ratio of cell culture well are 0.8～1.2, institute
The ratio stating the degree of depth of reservoir and the degree of depth of described cell culture well is 0.8～1.2, reservoir upper
Surface and the distance of the upper surface flush of cell culture well, described reservoir and described plate body outer rim
Being 1～5mm, described reservoir is 3～6mm with the distance of described outer rim cell culture well.
(3) beneficial effect
Tissue Culture Plate described in the utility model, has the advantages that
1) this product remains the basic structure of tradition culture plate, is newly provided with one at its edge
It is specifically designed to the groove adding the sterile liquid such as PBS or distilled water week, for " protection " Internal Experiment
Hole, will not cause the evaporation of liquid in the cell culture well at edge, and with traditional solution this
The method of one problem is compared, and is not required to sacrifice border cell's culture hole, saves cell culture well.
The reservoir described in the utility model protection philosophy to cell culture well and the evaporation of liquid
Relevant, fluid molecule is affected by ambient energy can leave liquid level, enters in air, at liquid
The top of body forms vapour pressure, and these steams can spread to surrounding, also can collide between fluid molecule,
After consumed energy or be directly hit back liquid level, or it is diffused in surrounding.It is in cell
The steam that liquid in the hole at culture plate edge is formed is more easy to by the seam between plate lid and plate body
Gap diffuses in air, and vapor pressure lowering is fast, and the hole of inside, fluid molecule is the most mutual
The chance of collision is big, the most easily returns in liquid, and vapour pressure is more stable.This product sets up week
Limit liquid bath, can replace edge hole, and the vapour pressure of internal holes is risen Stabilization.
2) this utility model selects to use the structure of reservoir, cultivates with traditional use cell
The method of the outer rim cell culture well of plate is compared, the interpolation of liquid convenient to operation, and
The waste of cell culture well will not be caused.
3) reservoir described in the utility model and cell culture well are provided with a certain distance, with
Traditional directly uses the outermost layer sub-layers cell culture well of Tissue Culture Plate as protective layer phase
Ratio, liquid is not easily accessible cell culture well, can reduce the error of experiment.
In a word, Tissue Culture Plate of the present invention, both can play the effect to prevention edge effect,
The most easy to operate, prevent liquid from entering cell culture well, reduce experimental error.
Accompanying drawing explanation
Fig. 1 is elevation cross-sectional view of the present utility model；
Fig. 2 is top plan view of the present utility model.
In figure: 1, plate body；2, lid；3, cell culture well；4, reservoir；5, plate
Body skeleton；6, identification post；7, plate body outer rim.
Detailed description of the invention
Following example are used for illustrating this utility model, but are not limited to model of the present utility model
Enclose.
Embodiment 1
The present embodiment relates to the Tissue Culture Plate preventing edge effect described in the utility model,
As in figure 2 it is shown, on the plate body 1 of described Tissue Culture Plate, arrange around outer rim cell culture well
Reservoir 4.
Embodiment 2
The present embodiment relates to the Tissue Culture Plate preventing edge effect described in the utility model,
As it is shown in figure 1, compared with embodiment 1, its difference is:
The width of described reservoir is 0.8 with the diameter ratio of cell culture well 3；
The degree of depth of described reservoir 4 is 0.8 with the ratio of the cell culture well degree of depth；
The upper surface of reservoir 4 and the upper surface flush of cell culture well 3, described lid 2 is placed on
On described plate body 1；
The distance with plate body outer rim 7 of reservoir 4 is 3mm, the cell training of reservoir and outer most edge
Support hole distance be 6mm, described reservoir be corner be four limit cannelures of fillet.
Embodiment 3
Tissue Culture Plate described in the utility model, including following operating procedure:
1. experimenter tears packaging, takes out Tissue Culture Plate.
Open plate lid 2 the most gently, and empirically need the experiment done to complete cell culture well 3
Operation.
3. draw 8～the sterile liquid such as 10mlPBS or distilled water, from week with pipet or liquid-transfering gun
It is slowly added in limit liquid bath 4 one, is added to about the 1/2 of neighboring liquid groove depth.
4. covering plate lid (2), experimenter carries out next step experiment.
5. all should operate in an aseptic environment more than.
Although, used general explanation, detailed description of the invention and test, to this reality
Made detailed description with novel, but on the basis of this utility model, can to work some repair
Changing or improve, this will be apparent to those skilled in the art.Therefore, without departing from
These modifications or improvements on the basis of this utility model spirit, belong to this utility model
Claimed scope.
Claims (8)
1. the Tissue Culture Plate preventing edge effect, it is characterised in that on the plate body of described Tissue Culture Plate, arranges the reservoir around outer rim cell culture well.
Tissue Culture Plate the most according to claim 1, it is characterised in that the width of described reservoir and the diameter ratio of cell culture well are 0.8～1.2.
Tissue Culture Plate the most according to claim 1 and 2, it is characterised in that the degree of depth of described reservoir is 0.8～1.2 with the ratio of the degree of depth of described cell culture well.
Tissue Culture Plate the most according to claim 3, it is characterised in that the upper surface of described reservoir and the upper surface flush of described cell culture well.
5. according to the Tissue Culture Plate described in claim 1 or 4, it is characterised in that described reservoir is 1～5mm with the distance of described plate body outer rim.
6. according to the Tissue Culture Plate described in claim 1 or 4, it is characterised in that described reservoir is 3～6mm with the distance of described outer rim cell culture well.
7. according to the Tissue Culture Plate described in claim 1 or 4, it is characterised in that the corner of described reservoir is fillet.
8. the Tissue Culture Plate described in claim 1, it is characterized in that, the width of described reservoir and the diameter ratio of cell culture well are 0.8～1.2, the degree of depth of described reservoir is 0.8～1.2 with the ratio of the degree of depth of described cell culture well, the upper surface of reservoir and the upper surface flush of cell culture well, described reservoir is 1～5mm with the distance of described plate body outer rim, and described reservoir is 3～6mm with the distance of described outer rim cell culture well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620220909.2U CN205710766U (en) | 2016-03-22 | 2016-03-22 | A kind of Tissue Culture Plate preventing edge effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620220909.2U CN205710766U (en) | 2016-03-22 | 2016-03-22 | A kind of Tissue Culture Plate preventing edge effect |
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| Publication Number | Publication Date |
|---|---|
| CN205710766U true CN205710766U (en) | 2016-11-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620220909.2U Expired - Fee Related CN205710766U (en) | 2016-03-22 | 2016-03-22 | A kind of Tissue Culture Plate preventing edge effect |
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| CN (1) | CN205710766U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707551A (en) * | 2018-06-27 | 2018-10-26 | 深圳市深研生物科技有限公司 | A kind of cell observation device and cell observation method |
-
2016
- 2016-03-22 CN CN201620220909.2U patent/CN205710766U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707551A (en) * | 2018-06-27 | 2018-10-26 | 深圳市深研生物科技有限公司 | A kind of cell observation device and cell observation method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161123 Termination date: 20170322 |