CN205699591U - A kind of novel combination type column chromatography device - Google Patents

Abstract

The open a kind of novel combination type column chromatography device of this utility model, including: chromatographic column, Y tube, constant voltage separatory funnel and pressure tap, the lower end interface link layer analysis post of Y tube, the upper end interface of two of Y tube connects constant voltage separatory funnel and pressure tap respectively；Pressure air imports chromatographic column and constant voltage separatory funnel by Y tube after pressure tap pressure regulation, promote the eluent that in constant voltage separatory funnel, action of gravity flows down, mixture in chromatographic column realizes eluting separate, the evacuated tube with piston structure arranged by the upper end of pressure tap carries out pressure release, change the eluent in constant voltage separatory funnel, recover pressurization, it is achieved gradient elution.This apparatus structure is simple, feature richness, easy to use, saving reagent, achieve normal pressure, pressurization, isocratic elution, gradient elution, liquid-auto-dropping, speed is adjustable, and the function of detector can be connected, and this device can be widely applied to the chromatographic isolation field of laboratory, institute and production enterprise.

Description

A kind of novel combination type column chromatography device

Technical field

This utility model relates to a kind of novel combination type column chromatography device.

Background technology

Column chromatography, also known as column chromatography, is a kind of specific implementation of chromatographic isolation, column chromatography Separation is to be filled in fixing mutually in cylinder, loads mixture to be separated, uses single or mixed Close or change over flowing and flow through fixing phase mutually, due to component each in mixture and fixing phase Flowing phase separation power varies in size and translational speed is different, finally realize different material separation and Purification.Now conventional fixing phase granularity is less, manually adds flowing phase, flows and flow mutually under normal pressure Speed is little, flows and be difficult to mutually change in separation process, and detection means is single, and reagent waste is many, Separating extremely time-consuming, labor intensity is big.

Utility model content

For above-mentioned technical problem, this utility model provides a kind of novel combination type column chromatography dress Put, can conveniently realize that pressure is variable, eluent convenient switching, liquid-auto-dropping, speed is adjustable, Can the function of external detector.

This utility model be the technical scheme is that

A kind of novel combination type column chromatography device, including: chromatographic column, Y tube, constant voltage are divided Liquid funnel and pressure tap, the lower end interface link layer analysis post of described Y tube, described Y shape The upper end interface of two of pipe connects constant voltage separatory funnel and pressure tap respectively；

On the outer wall of described chromatographic column, scale is set；The inside of described chromatographic column is fixedly installed one Porous sand plate, the bottom of described chromatographic column arranges the first rotary-piston, in order to control aperture； The upper end interface of described chromatographic column is inner side frosted type standard interface；The upper end of described chromatographic column The outside of interface is provided with chromatographic column barb；The bottom of described first rotary-piston connects chromatographic column Tip, outside described chromatographic column tip is flat taper, and the outer wall of described chromatographic column tip is arranged Screw thread；

The lower end of described constant voltage separatory funnel arranges the second rotary-piston, is used for controlling aperture, The body that expands on the top of described second rotary-piston is liquid storage body, described liquid storage body Arranging scale on outer wall, the bottom of described second rotary-piston is outside frosted type standard interface, The upper end interface of described constant voltage separatory funnel is inner side frosted type standard interface；Described constant voltage is divided On the upper end interface of liquid funnel, hollow glass plug is set；The upper termination of described constant voltage separatory funnel The outside of mouth also sets up hook, and described hook is fixed by rubber band with chromatographic column barb；

The lower end interface of described pressure tap is outside frosted type standard interface, and described pressure connects The upper end of pipe is set to the evacuated tube with piston structure；The side of described pressure tap is arranged Pressed gas entrance, described pressed gas entrance has ladder disconnecting prevention structure.

The beneficial effects of the utility model are that described modular layer analysis apparatus can be according to concrete need Column chromatography pressure to be realized is variable, eluent facilitates switching, liquid-auto-dropping, speed is adjustable, Connect the function of detector detection, and apparatus structure is simple, feature richness, easy to use, It is greatly improved mask work efficiency.

Accompanying drawing explanation

Fig. 1 is the structural representation of novel combination type column chromatography device described in the utility model；

In figure, 1, chromatographic column；2, Y tube；3, constant voltage separatory funnel；4, pressure connects Pipe；11, chromatographic column tip；12, porous sand plate；13, the first rotary-piston；14, color Spectrum post barb；31, the second rotary-piston；32, hollow glass plug；33, hook；41、 Pressed gas entrance.

Detailed description of the invention

Below in conjunction with the accompanying drawings this utility model is described in further detail, to make this area Technical staff can implement according to this with reference to description word.

As it can be seen, the open a kind of novel combination type column chromatography device of this utility model, including: Chromatographic column 1, Y tube 2, constant voltage separatory funnel 3 and pressure tap 4, described Y tube 2 Lower end interface link layer analysis post 1, the upper end interface of two of described Y tube 2 connects respectively Constant voltage separatory funnel 3 and pressure tap 4；

On the outer wall of described chromatographic column 1, scale is set；The inside of described chromatographic column 1 is fixing to be set Putting a porous sand plate 12, the bottom of described chromatographic column 1 arranges the first rotary-piston 13, in order to Control aperture；The upper end interface of described chromatographic column 1 is inner side frosted type standard interface；Described The outside of the upper end interface of chromatographic column 1 is provided with chromatographic column barb 14；Described first rotary-piston The bottom of 13 connects chromatography column jecket point 11, and described chromatographic column tip 11 is flat taper, and institute The outer wall stating chromatographic column tip 11 arranges external screw thread；

The lower end of described constant voltage separatory funnel 3 arranges the second rotary-piston 31, opens for control Degree, the body that expands on the top of described second rotary-piston 31 is liquid storage body, described liquid storage Arranging scale on the outer wall of body, the bottom of described second rotary-piston 31 is outside frosted type Standard interface, the upper end interface of described constant voltage separatory funnel 3 is inner side frosted type standard interface； On the upper end interface of described constant voltage separatory funnel 3, hollow glass plug 32 is set；Described constant voltage is divided The outside of the upper end interface of liquid funnel 3 also sets up hook 33, described hook 33 and chromatographic column Barb 14 is fixed by rubber band；

The lower end interface of described pressure tap 4 is outside frosted type standard interface, described pressure The upper end of adapter 4 is set to the evacuated tube with piston structure；The one of described pressure tap 4 Side arranges pressed gas entrance 41, and described pressed gas entrance 41 has ladder disconnecting prevention structure.

Concrete operation step includes:

A, preparation: select chromatographic column 1 and the leakage of constant voltage separatory of suitable dimension size as required Bucket 3, cleaning is dried；Dry column-packing then carries out necessary drying mutually by fixing, and wet method dress post is then To fix accordingly and infiltrate swelling preparation mutually；Conventional fixing have mutually silica gel, activated carbon, aluminium oxide, Kieselguhr, cellulose, gel, macroporous resin, polyamide, ion exchange resin；

B, dress post (as a example by wet method): fixing chromatographic column 1, add appropriate quartz with little funnel Sand makes to be formed filter course on porous sand plate 12, adds fixing phase, opens piston and waits for sedimentation quietly, Pressurization Accelerated subsidence make fixing accumulation mutually closely if desired, adds the most even and regular when liquid level is relatively low Amount quartz sand also makes quartz sand smooth, loads mixed liquor, and a small amount of reagent cleans chromatographic column 1 wall；

C, eluting: normal pressure eluting is installed and filled the constant voltage separatory funnel 3 of flowing phase and adjust work Plug aperture, makes liquid droping speed consistent with chromatographic column tip 11 rate of outflow；Pressurization eluting is installed Fill constant voltage separatory funnel 3 and pressure tap 4, the pressure gas of pressure tap 4 of flowing phase Body entrance 41 is connected to air compressor machine pressurization, with rubber band or snap-lock chromatographic column 1, Y shape Pipe 2, constant voltage separatory funnel 3 and pressure tap 4, pressure tap 4 piston standard-sized sheet, open sky Press pump is pressurizeed by pressed gas entrance 41, and adjustment piston, to predetermined pressure, adjusts work in time Filling in 31 apertures, liquid droping speed is consistent with chromatographic column tip 11 rate of outflow；Change flowing phase (gradient elution or pressurization isocratic elution) Shi Douying first slowly opens pressure tap 4 vent piston Pressure, pipettes constant voltage separatory funnel 3 after pressure balance and changes flowing phase, again after binding again Start the eluting that pressurizes；No matter which kind of eluting, be always ensured that chromatographic column 1 upper strata quartz sand layer with On remain with the flowing phase of certain altitude, it is to avoid the air that dries up when flowing is not enough mutually enters fixing Cause mutually and separate unsuccessfully；

D, reception: effluent introduces detector at chromatographic column tip 11, believe according to detector Number change is received；Simple separation coloring matter can move visually segmentation according to colour band and connect Receive.

Modular layer analysis apparatus described in the utility model can realize column chromatography according to specific needs Pressure is variable, eluent facilitates switching, liquid-auto-dropping, speed is adjustable, connect detector inspection The function surveyed, and apparatus structure is simple, feature richness, easy to use, be greatly improved point From work efficiency.

Although embodiment of the present utility model is disclosed as above, but it is not restricted to Listed utilization in bright book and embodiment, it can be applied to various this practicality applicable completely Novel field, for those skilled in the art, is easily achieved other repairing Change, therefore under the general concept limited without departing substantially from claim and equivalency range, this reality It is not limited to specific details and shown here as the legend with description with novel.

Claims (1)

1. a novel combination type column chromatography device, it is characterised in that including: chromatographic column, Y tube, constant voltage separatory funnel and pressure tap, the lower end interface link layer of described Y tube Analysis post, the upper end interface of two of described Y tube connects constant voltage separatory funnel respectively and pressure connects Pipe；

On the outer wall of described chromatographic column, scale is set；The inside of described chromatographic column is fixedly installed one Porous sand plate, the bottom of described chromatographic column arranges the first rotary-piston, in order to control aperture； The upper end interface of described chromatographic column is inner side frosted type standard interface；The upper end of described chromatographic column The outside of interface is provided with chromatographic column barb；The bottom of described first rotary-piston connects chromatographic column Tip, outside described chromatographic column tip is flat taper, and the outer wall of described chromatographic column tip is arranged Screw thread；

The lower end of described constant voltage separatory funnel arranges the second rotary-piston, is used for controlling aperture, The body that expands on the top of described second rotary-piston is liquid storage body, described liquid storage body Arranging scale on outer wall, the bottom of described second rotary-piston is outside frosted type standard interface, The upper end interface of described constant voltage separatory funnel is inner side frosted type standard interface；Described constant voltage is divided On the upper end interface of liquid funnel, hollow glass plug is set；The upper termination of described constant voltage separatory funnel The outside of mouth also sets up hook, and described hook is fixed by rubber band with chromatographic column barb；

The lower end interface of described pressure tap is outside frosted type standard interface, and described pressure connects The upper end of pipe is set to the evacuated tube with piston structure；The side of described pressure tap is arranged Pressed gas entrance, described pressed gas entrance has ladder disconnecting prevention structure.