CN205699586U - A kind of modified model combination type column chromatography device - Google Patents

Abstract

The open a kind of modified model combination type column chromatography device of this utility model, including: chromatographic column, constant voltage separatory funnel, pressure tap and surge flask；Chromatographic column and constant voltage separatory funnel is imported by pressure tap again after pressure air buffered bottle pressure regulation pressure measurement, promote the eluent flowed down by gravity in constant voltage separatory funnel, mixture in chromatographic column realizes eluting separate, and change the eluent in constant voltage separatory funnel by surge flask pressure release, recover pressurization, it is achieved gradient elution.This apparatus structure is simple, feature richness, easy to use, saving reagent, achieve normal pressure, pressurization, isocratic elution, gradient elution, liquid-auto-dropping, speed is adjustable, and the function of detector can be connected, and this device can be widely applied to the chromatographic isolation field of laboratory, institute and production enterprise.

Description

A kind of modified model combination type column chromatography device

Technical field

This utility model relates to a kind of modified model combination type column chromatography device.

Background technology

Column chromatography, also known as column chromatography, is a kind of specific implementation of chromatographic isolation, column chromatography Separation is to be filled in fixing mutually in cylinder, loads mixture to be separated, uses single or mixed Close or change over flowing and flow through fixing phase mutually, due to component each in mixture and fixing phase Flowing phase separation power varies in size and translational speed is different, finally realize different material separation and Purification.Now conventional fixing phase granularity is less, manually adds flowing phase, flows and flow mutually under normal pressure Speed is little, flows and be difficult to mutually change in separation process, and detection means is single, and reagent waste is many, Separating extremely time-consuming, labor intensity is big.

Utility model content

For above-mentioned technical problem, this utility model provides a kind of modified model combination type column chromatography Device, can conveniently realize that pressure is variable, eluent convenient switching, liquid-auto-dropping, speed can Adjust, can the function of external detector.

This utility model be the technical scheme is that

A kind of modified model combination type column chromatography device, including: chromatographic column, constant voltage separatory funnel, Pressure tap and surge flask；

On the outer wall of described chromatographic column, scale is set；The inside of described chromatographic column is fixedly installed one Porous sand plate, the bottom of described chromatographic column arranges the first rotary-piston, in order to control aperture； The upper end interface of described chromatographic column is inner side frosted type standard interface；The upper end of described chromatographic column The outside of interface is provided with chromatographic column barb；The bottom of described first rotary-piston connects chromatographic column Tip, outside described chromatographic column tip is flat taper, and the outer wall of described chromatographic column tip is arranged Screw thread；

The lower end of described constant voltage separatory funnel arranges the second rotary-piston, is used for controlling aperture, The body that expands on the top of described second rotary-piston is liquid storage body, described liquid storage body Arranging scale on outer wall, the bottom of described second rotary-piston is outside frosted type standard interface, The upper end interface of described constant voltage separatory funnel is inner side frosted type standard interface；Described constant voltage is divided On the upper end interface of liquid funnel, hollow glass plug is set；The upper termination of described constant voltage separatory funnel The outside of mouth also sets up hook, and described hook is fixed by rubber band with chromatographic column barb；

The lower end interface of described pressure tap is outside frosted type standard interface, and described pressure connects The upper end interface of pipe is inner side frosted type standard interface, and the side of described pressure tap arranges side Pressure interface, described lateral pressure interface has ladder disconnecting prevention structure；Described lateral pressure interface leads to Cross detachable pressure line to be connected with surge flask；

The bottle mouth position of described surge flask arranges stopper, and described stopper inserts pressure-measuring pipe and emptying Pipe, described evacuated tube arranges the 3rd rotary-piston, and the both sides of described surge flask are respectively provided with One pressed gas gateway, described pressed gas gateway has ladder disconnecting prevention structure.

The beneficial effects of the utility model are that described modular layer analysis apparatus can be according to concrete need Column chromatography pressure to be realized is variable, eluent facilitates switching, liquid-auto-dropping, speed is adjustable, Connect the function of detector detection, and apparatus structure is simple, feature richness, easy to use, It is greatly improved mask work efficiency.

Accompanying drawing explanation

Fig. 1 is the structural representation of modified model combination type column chromatography device described in the utility model Figure；

In figure, 1, chromatographic column；2, constant voltage separatory funnel；3, pressure tap；4, buffering Bottle；11, chromatographic column tip；12, porous sand plate；13, the first rotary-piston；14, color Spectrum post barb；21, the second rotary-piston；22, hollow glass plug；23, hook；31、 Lateral pressure interface；41, pressed gas gateway；42, evacuated tube；43, pressure-measuring pipe；44、 3rd rotary-piston.

Detailed description of the invention

Below in conjunction with the accompanying drawings this utility model is described in further detail, to make this area Technical staff can implement according to this with reference to description word.

As it can be seen, the open a kind of modified model combination type column chromatography device of this utility model, bag Include: chromatographic column 1, constant voltage separatory funnel 2, pressure tap 3 and surge flask 4；

On the outer wall of described chromatographic column 1, scale is set；The inside of described chromatographic column 1 is fixing to be set Putting a porous sand plate 12, the bottom of described chromatographic column 1 arranges the first rotary-piston 13, in order to Control aperture；The upper end interface of described chromatographic column 1 is inner side frosted type standard interface；Described The outside of the upper end interface of chromatographic column 1 is provided with chromatographic column barb 14；Described first rotary-piston The bottom of 13 connects chromatography column jecket point 11, and described chromatographic column tip 11 is flat taper, and institute The outer wall stating chromatographic column tip 11 arranges external screw thread；

The lower end of described constant voltage separatory funnel 2 arranges the second rotary-piston 21, opens for control Degree, the body that expands on the top of described second rotary-piston 21 is liquid storage body, described liquid storage Arranging scale on the outer wall of body, the bottom of described second rotary-piston 21 is outside frosted type Standard interface, the upper end interface of described constant voltage separatory funnel 2 is inner side frosted type standard interface； On the upper end interface of described constant voltage separatory funnel 2, hollow glass plug 22 is set；Described constant voltage is divided The outside of the upper end interface of liquid funnel 2 also sets up hook 23, described hook 23 and chromatographic column Barb 14 is fixed by rubber band；

The lower end interface of described pressure tap 3 is outside frosted type standard interface, described pressure The upper end interface of adapter 3 is inner side frosted type standard interface, the side of described pressure tap 3 Arranging lateral pressure interface 31, described lateral pressure interface 31 has ladder disconnecting prevention structure；Described Lateral pressure interface 31 is connected with surge flask 4 by detachable pressure line；

The bottle mouth position of described surge flask 4 arranges stopper, and described stopper inserts pressure-measuring pipe 43 With evacuated tube 42, described evacuated tube 42 arranges the 3rd rotary-piston 44, described surge flask The both sides of 4 are respectively provided with a pressed gas gateway 41, described pressed gas gateway 41 There is ladder disconnecting prevention structure.

Concrete operation step includes:

A, preparation: select chromatographic column 1 and the leakage of constant voltage separatory of suitable dimension size as required Bucket 2, cleaning is dried；Dry column-packing then carries out necessary drying mutually by fixing, and wet method dress post is then To fix accordingly and infiltrate swelling preparation mutually；Conventional fixing have mutually silica gel, activated carbon, aluminium oxide, Kieselguhr, cellulose, gel, macroporous resin, polyamide, ion exchange resin；

B, dress post (as a example by wet method): fixing chromatographic column 1, add appropriate quartz with little funnel Sand makes to be formed filter course on porous sand plate 12, adds fixing phase, opens the first rotary-piston 13 wait for sedimentation quietly, if desired pressurization Accelerated subsidence make fixing to pile up closely mutually, and liquid level is relatively low Time add uniform appropriate amount of quartz sand and make quartz sand smooth, load mixed liquor, a small amount of reagent is clear Wash chromatographic column 1 wall；

C, eluting: normal pressure eluting is installed and filled the flowing constant voltage separatory funnel 2 of phase and adjust the Two rotary-piston 21 apertures, make liquid droping speed consistent with chromatographic column tip 11 rate of outflow； Pressurization eluting installs constant voltage separatory funnel 2 and the pressure tap 3 filling flowing phase, and pressure connects Pipe 3 lateral pressure interface is connected to pressed gas gateway 41, with rubber band or buckle fixed layer Analysis post 1, pressure tap 3 and constant voltage separatory funnel 2, the 3rd rotary-piston 44 standard-sized sheet, open Open air pressure pump to be pressurizeed by pressed gas gateway 41, adjust the 3rd rotary-piston 44 in advance Constant-pressure, adjusts the second rotary-piston 21 aperture, liquid droping speed and chromatographic column tip in time 11 rates of outflow are consistent；Change flowing phase (gradient elution or pressurization isocratic elution) Shi Douying First pressure release, pipettes constant voltage separatory funnel 2 after pressure balance and changes flowing phase, again bind After start the eluting that pressurizes again；No matter which kind of eluting, is always ensured that the quartz on chromatographic column 1 upper strata More than layer of sand remain with the flowing phase of certain altitude, it is to avoid the air that dries up during flowing deficiency mutually enters Enter to fix to cause mutually and separate unsuccessfully；

D, reception: effluent introduces detector at chromatographic column tip 11, according to detector Signal intensity is received；Simple separation coloring matter can move visually segmentation according to colour band Receive.

Modular layer analysis apparatus described in the utility model can realize column chromatography according to specific needs Pressure is variable, eluent facilitates switching, liquid-auto-dropping, speed is adjustable, connect detector inspection The function surveyed, and apparatus structure is simple, feature richness, easy to use, be greatly improved point From work efficiency.

Although embodiment of the present utility model is disclosed as above, but it is not restricted to Listed utilization in bright book and embodiment, it can be applied to various this practicality applicable completely Novel field, for those skilled in the art, is easily achieved other repairing Change, therefore under the general concept limited without departing substantially from claim and equivalency range, this reality It is not limited to specific details and shown here as the legend with description with novel.

Claims (1)

1. a modified model combination type column chromatography device, it is characterised in that including: chromatographic column, Constant voltage separatory funnel, pressure tap and surge flask；

On the outer wall of described chromatographic column, scale is set；The inside of described chromatographic column is fixedly installed one Porous sand plate, the bottom of described chromatographic column arranges the first rotary-piston, in order to control aperture； The upper end interface of described chromatographic column is inner side frosted type standard interface；The upper end of described chromatographic column The outside of interface is provided with chromatographic column barb；The bottom of described first rotary-piston connects chromatographic column Tip, outside described chromatographic column tip is flat taper, and the outer wall of described chromatographic column tip is arranged Screw thread；

The lower end of described constant voltage separatory funnel arranges the second rotary-piston, is used for controlling aperture, The body that expands on the top of described second rotary-piston is liquid storage body, described liquid storage body Arranging scale on outer wall, the bottom of described second rotary-piston is outside frosted type standard interface, The upper end interface of described constant voltage separatory funnel is inner side frosted type standard interface；Described constant voltage is divided On the upper end interface of liquid funnel, hollow glass plug is set；The upper termination of described constant voltage separatory funnel The outside of mouth also sets up hook, and described hook is fixed by rubber band with chromatographic column barb；

The lower end interface of described pressure tap is outside frosted type standard interface, and described pressure connects The upper end interface of pipe is inner side frosted type standard interface, and the side of described pressure tap arranges side Pressure interface, described lateral pressure interface has ladder disconnecting prevention structure；Described lateral pressure interface leads to Cross detachable pressure line to be connected with surge flask；

The bottle mouth position of described surge flask arranges stopper, and described stopper inserts pressure-measuring pipe and emptying Pipe, described evacuated tube arranges the 3rd rotary-piston, and the both sides of described surge flask are respectively provided with One pressed gas gateway, described pressed gas gateway has ladder disconnecting prevention structure.