CN205603607U - Be suitable for allele high resolution fusion curve analysis and detection kit - Google Patents
Be suitable for allele high resolution fusion curve analysis and detection kit Download PDFInfo
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- CN205603607U CN205603607U CN201620438490.8U CN201620438490U CN205603607U CN 205603607 U CN205603607 U CN 205603607U CN 201620438490 U CN201620438490 U CN 201620438490U CN 205603607 U CN205603607 U CN 205603607U
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Abstract
The utility model provides a be suitable for allele high resolution fusion curve analysis and detection kit, including the lid, box body and the bottom low temperature control module who suits, the liner is adopted on the upper portion surface of box body, the lower part is adopted the square groove to separate and is provided with seven containers constitution bottom low temperature control module respectively, bottom low temperature control module is including the vessel bore who places PCR mix, place the vessel bore that the primer is mixed to high resolution fusion tracing analysis specificity, place deionized water's vessel bore, place the vessel bore of allele's DNA standard substance no. 1, place the vessel bore of allele's DNA standard substance no. 2, place the vessel bore of allele's DNA standard substance no. 3 and the container of placing sterilization PCR reaction tube, the lower part of box body sets up the low temperature control space of cutting apart into four differences for the cryosphere, is cryosphere no. 1, cryosphere no. 2, cryosphere no. 3 respectively and places the container of sterilization PCR reaction tube, SNP somatotype accuracy can be guaranteed to this kit, can reach reagent temperature sensitive's requirement simultaneously.
Description
Technical field
This utility model relates to the technical field analyzing test kit device, and more specifically, this utility model relates to the technical field of a kind of allele high-resolution melting curve analysis detection.
Background technology
Rs1800629 allele high-resolution melting curve analysis detection kit is for by contrasting the polymorphism in temperature changing process of the gene on crowd's chromosome, the waveform peak difference being i.e. embodied on melting curve, come ratio shared by specific site difference base and a certain character or the dependency of disease in analysis crowd, for further genescreen, the research of the aspect such as disease prevention and clinical application guidance lays the foundation.But owing to operation is more complicated, be easier contaminated, more sensitive to temperature, complex structure and other problems.Therefore, need badly and existing high-resolution melting curve analysis detection kit is carried out upgrading, overcome the problem and shortage of existing existence.
Summary of the invention
Deficiency and the problem of existence for prior art present situation, this utility model provides one to be suitable for allele high-resolution melting curve analysis detection kit, compared with prior art, the applicable allele high-resolution melting curve analysis detection kit that this utility model provides is applicable to the detection of rs1800629 allele high-resolution melting curve analysis, simple in construction, reasonable in design, it is convenient for carrying transport.Ensureing while SNP typing accuracy, it is achieved that the advantage such as simple, quick, with low cost, greatly meeting the needs of gene studies level.
For achieving the above object, the technical solution adopted in the utility model is:
This utility model provides one to be suitable for allele high-resolution melting curve analysis detection kit, including lid, box body and the bottom low temperature control module adapted, the upper face of box body uses liner, bottom uses square groove to separate and is respectively arranged with low temperature control module bottom seven container compositions, bottom low temperature control module includes the container hole placing PCR mix, place the container hole of high-resolution melting curve analysis specificity mix primer, place the container hole of deionized water, place the container hole of allelic DNA standard substance one, place the container hole of allelic DNA standard substance two, place the container hole of allelic DNA standard substance three and place the container of sterilizing PCR reaction tube；The bottom of box body is set to cryosphere and is divided into four different low temperature control spaces, respectively cryosphere one, cryosphere two, cryosphere three and the container of placement sterilizing PCR reaction tube；The container hole placing PCR mix is correspondingly arranged with cryosphere one, the container hole placing high-resolution melting curve analysis specificity mix primer and the container hole placing deionized water are correspondingly arranged with cryosphere two, the container hole placing allelic DNA standard substance one, the container hole placing allelic DNA standard substance two and place the container hole of allelic DNA standard substance three and be correspondingly arranged with common cryosphere three.
In this utility model, place and the container hole of PCR mix is placed PCR mix, place and the container hole of high-resolution melting curve analysis specificity mix primer is placed high-resolution melting curve analysis specificity mix primer, place and the container hole of deionized water is placed deionized water, place the container hole of allelic DNA standard substance one, the container hole placing allelic DNA standard substance two places three different allelic DNA standard substance in the container hole placing allelic DNA standard substance three respectively, this utility model preferentially uses rs1800629 allele, place and the container of sterilizing PCR reaction tube is placed sterilizing PCR reaction tube, the container hole square groove of three kinds of allelic DNA standard substance separates, so that distinguishing, accelerate work efficiency.The bottom cryosphere of cassette bottom is divided into four, it is respectively cryosphere one, cryosphere two and cryosphere three, control temperature and the condition adapted therewith respectively, it is required for temperature, humidity and aridity that bottom low temperature control module provides different, meets the requirement being suitable for the detection of allele high-resolution melting curve analysis with this.
The employing technique scheme following beneficial effect of acquisition:
This utility model is used to provide one to be suitable for allele high-resolution melting curve analysis detection kit, simple in construction, reasonable in design, it is convenient for carrying transport.Ensureing while SNP typing accuracy, it is achieved that the advantage such as simple, quick, with low cost, can be simultaneously reached the requirement that temperature of reagent is sensitive.
Accompanying drawing explanation
Fig. 1 is shown as this utility model offer and is suitable for the box body structure schematic diagram in allele high-resolution melting curve analysis detection kit.
Fig. 2 is shown as this utility model of Fig. 1 and provides the front section view being suitable for allele high-resolution melting curve analysis detection kit.
Fig. 3 is shown as this utility model and provides the structural representation of the lid being suitable in allele high-resolution melting curve analysis detection kit.
In Fig. 1-3,1-places the container hole of high-resolution melting curve analysis specificity mix primer, 2-places the container hole of PCR mix, 3-liner, 4-places the container hole of deionized water, 5-places the container hole of allelic DNA standard substance one, 6-places the container hole of allelic DNA standard substance two, and 7-places the container hole of allelic DNA standard substance three, and 8-places the container of sterilizing PCR reaction tube, 9-box body, low temperature control module bottom 10-, 11-cryosphere one, 12-cryosphere two, 13-cryosphere three, 14-lid.
Detailed description of the invention
In conjunction with accompanying drawing 1-3, illustrate for embodiment, but, this utility model is not limited to following embodiment.
nullThis utility model provides one to be suitable for allele high-resolution melting curve analysis detection kit，Including lid (14)、Box body (9) and bottom low temperature control module (10) adapted，The upper face of box body (9) uses liner (3)，Bottom uses square groove to separate and is respectively arranged with low temperature control module (10) bottom seven container compositions，Bottom low temperature control module (10) includes the container hole (2) placing PCR mix、Place the container hole (1) of high-resolution melting curve analysis specificity mix primer、Place the container hole (4) of deionized water、Place the container hole (5) of allelic DNA standard substance one、Place the container hole (6) of allelic DNA standard substance two、Place the container hole (7) of allelic DNA standard substance three and place the container (8) of sterilizing PCR reaction tube；The bottom of box body (9) is set to cryosphere and is divided into four different low temperature control spaces, respectively cryosphere one (11), cryosphere two (12), cryosphere three (13) and the container (8) of placement sterilizing PCR reaction tube；The container hole (2) placing PCR mix is correspondingly arranged with cryosphere one (11), the container hole (1) placing high-resolution melting curve analysis specificity mix primer and the container hole (4) placing deionized water are correspondingly arranged with cryosphere two (12), and the container hole (7) of the container hole (5) placing allelic DNA standard substance one, the container hole (6) placing allelic DNA standard substance two and the allelic DNA standard substance three of placement is correspondingly arranged with common cryosphere three (13).
nullIn this utility model，Place and the container hole (2) of PCR mix is placed PCR mix，Place and the container hole (1) of high-resolution melting curve analysis specificity mix primer is placed high-resolution melting curve analysis specificity mix primer，Place and the container hole (4) of deionized water is placed deionized water，Place the container hole (5) of allelic DNA standard substance one、The container hole (6) placing allelic DNA standard substance two places three different allelic DNA standard substance in the container hole (7) placing allelic DNA standard substance three respectively，This utility model preferentially uses rs1800629 allele，Place and the container (8) of sterilizing PCR reaction tube is placed sterilizing PCR reaction tube，The container hole square groove of three kinds of allelic DNA standard substance separates，So that distinguishing，Accelerate work efficiency.The bottom cryosphere of cassette bottom is divided into four, it is respectively cryosphere one (11), cryosphere two (12) and cryosphere three (13), control temperature and the condition adapted therewith respectively, it is required for temperature, humidity and aridity that bottom low temperature control module (10) provides different, meets the requirement being suitable for the detection of allele high-resolution melting curve analysis with this.
In sum, this utility model provides one to be suitable for allele high-resolution melting curve analysis detection kit, it is highly suitable for the detection of rs1800629 allele high-resolution melting curve analysis, simple in construction, reasonable in design, it is convenient for carrying transport, ensureing while SNP typing accuracy, it is achieved that simple, quick, with low cost, meet the advantages such as temperature of reagent is sensitive.
Although this utility model discloses as above with preferred embodiment, but this utility model is not limited to this.Any those skilled in the art, without departing from spirit and scope of the present utility model, all can make various changes or modifications; any amendment that is all within spirit of the present utility model and principle, that made, equivalent; improve, within should be included in protection domain of the present utility model.
Claims (1)
1. an applicable allele high-resolution melting curve analysis detection kit, it is characterized in that, including lid, box body and the bottom low temperature control module adapted, the upper face of box body uses liner, bottom uses square groove to separate and is respectively arranged with low temperature control module bottom seven container compositions, bottom low temperature control module includes the container hole placing PCR mix, place the container hole of high-resolution melting curve analysis specificity mix primer, place the container hole of deionized water, place the container hole of allelic DNA standard substance one, place the container hole of allelic DNA standard substance two, place the container hole of allelic DNA standard substance three and place the container of sterilizing PCR reaction tube；The bottom of box body is set to cryosphere and is divided into four different low temperature control spaces, respectively cryosphere one, cryosphere two, cryosphere three and the container of placement sterilizing PCR reaction tube；Place PCR
The container hole of mix is correspondingly arranged with cryosphere one, the container hole placing high-resolution melting curve analysis specificity mix primer and the container hole placing deionized water are correspondingly arranged with cryosphere two, the container hole placing allelic DNA standard substance one, the container hole placing allelic DNA standard substance two and place the container hole of allelic DNA standard substance three and be correspondingly arranged with common cryosphere three.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620438490.8U CN205603607U (en) | 2016-05-13 | 2016-05-13 | Be suitable for allele high resolution fusion curve analysis and detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620438490.8U CN205603607U (en) | 2016-05-13 | 2016-05-13 | Be suitable for allele high resolution fusion curve analysis and detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN205603607U true CN205603607U (en) | 2016-09-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620438490.8U Expired - Fee Related CN205603607U (en) | 2016-05-13 | 2016-05-13 | Be suitable for allele high resolution fusion curve analysis and detection kit |
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| CN (1) | CN205603607U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488586A (en) * | 2017-08-30 | 2017-12-19 | 合肥安为康医学检验有限公司 | A kind of intelligence system based on HRM technologies |
-
2016
- 2016-05-13 CN CN201620438490.8U patent/CN205603607U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488586A (en) * | 2017-08-30 | 2017-12-19 | 合肥安为康医学检验有限公司 | A kind of intelligence system based on HRM technologies |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160928 Termination date: 20210513 |