CN205599281U - A grind appearance ware for organizing grind appearance - Google Patents
A grind appearance ware for organizing grind appearance Download PDFInfo
- Publication number
- CN205599281U CN205599281U CN201620341354.7U CN201620341354U CN205599281U CN 205599281 U CN205599281 U CN 205599281U CN 201620341354 U CN201620341354 U CN 201620341354U CN 205599281 U CN205599281 U CN 205599281U
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- China
- Prior art keywords
- funnel
- mill
- chassis
- plug
- grind away
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model belongs to biotechnology apparatus field, in particular to mill appearance ware that is used for plant or animal tissue. By hanging mill (1), interlocking line (2), funnel (3) funnel stopper (4), it links to each other with the motor to hang mill (1) interlocking line (2), it cooperatees through screw thread funnel (3) to hang chassis (13) of mill (1) below, the upper portion outside of funnel (3) is equipped with perpendicular line (32), and interior sphere (33) of funnel (3) are smooth, and the outer bottom of funnel (3) is equipped with flare opening (34), and the outside of flare opening (34) is equipped with the band, the upper surface of funnel stopper (4) is the sphere, and the side is equipped with spring leaf (42), and flare opening (34) of funnel (3) bottom, the last sphere 41 of funnel stopper (4) and interior sphere (33) the coincidence of funnel (3) are filled in to funnel stopper (4) lock. The utility model discloses grind appearance thoroughly, the time is short, practice thrift sample and liquid nitrogen, reduces cross contamination's possibility, has eliminated the danger of sample cell explosion.
Description
Technical field
This utility model belongs to agricultural and biotech equipment field, particularly to a kind of for plant or the sample milling machine of animal tissue.
Background technology
Grind away is the single stepping frequently involved in the technical fields such as agricultural, specifically includes: agricultural, biotechnology, pharmacy, gardening etc., the pay attention to day by day tested biotechnology along with people, and grind away test application is more and more extensive.In grind away test, the grind away of tissue is one frequent and laborious operation of testing crew, such as in large quantities of extractions RNA (ribonucleic acid) or DNA (DNA (deoxyribonucleic acid)) are tested, need each experiment of each time point is repeated tissue grind away.Tissue can easily and fast, the grind away of health is directly connected to test schedule speed and final test effect.
The material of grind away experiment is mainly plant tissue and animal tissue, and period to ensure to organize activity or the integrity of interior molecular substance, so needing to use liquid nitrogen to keep sample to be in low temperature state, therefore particularly severe to test operation during tissue grind away.
There is following defect in tradition tissue grind away operation: (1) grind away time length and waste muscle power, general grind away mainly uses mortar, if thoroughly ground, need 20～30min, animal tissue is the thickest, and the required grind away time can be more long, and waste of manpower is the most；(2) sample waste is serious, typically need to be continuously added liquid nitrogen during grind away, fly away container adding the fashionable dust that often evokes, the most also have amount of powder and remain in the inwall of sample milling machine and cannot collect, for measuring less or precious sample, this waste is difficult to accept；(3) liquid nitrogen waste is serious, the typically liquid nitrogen to be continuously added when grind away, has when there is liquid nitrogen and grind in container, sample can flow everywhere with liquid nitrogen, so now the efficiency of grind away is low, only wait until that liquid nitrogen soon volatilizees most grind away, therefore has substantial amounts of liquid nitrogen and slatterns；(4) grind away is the most thorough, often through the color of powder, general grind away judges that grind away is the most complete, but the different times of different tissues or different tissues just cannot the most directly judge, this problem eliminates often by increasing the grind away time, so so the most indirectly causing defect 1), 2) and 3), typically hanging mill sample milling machine is grind away in 1.5ml conical centrifuge tube, due to centrifuge tube and the reason of rotary head, sample can not thoroughly be ground, sample can not thoroughly crush, and the material needed for may result in follow-up test can not effectively extract；5) there is cross-contamination, owing to grind away is to carry out in the container of opening, so when adding liquid nitrogen, the powder evoked can fly away everywhere, often through liquid nitrogen and capsule contamination to other test specimen, for requiring the strict test of comparison, such operation may result in test error；6) sample adds, with directly contacting of liquid nitrogen, the risk that sample stores, often with part liquid nitrogen when sample is stored into centrifuge tube, when temperature is increased to a certain degree, liquid nitrogen in centrifuge tube can be changed into gas, the pressure in centrifuge tube can be made to increase, so the risk that centrifuge tube explodes often occurs in follow-up test.
Utility model content
The purpose of this utility model is to solve common sample milling machine grind away time length, waste muscle power, waste sample and liquid nitrogen, grind away thoroughly and the problem of cross-contamination, it is provided that a kind of easy and simple to handle, save time, the tissue sample milling machine of health.It is characterized in that, tissue sample milling machine is by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section 1.5 meters long train lines (2).
The groove (11) preventing hands from sliding it is provided with at the described handle hanging mill (1), hang mill (1) lower section and be provided with chassis (13), chassis (13) is reinforced by reinforcement (12) with hanging mill (1), and the outside of chassis (13) is provided with external screw thread (14)；The rotary head (15) bottom center from chassis (13) is stretched out.
The upper inner of described funnel (3) is provided with female thread (31), and described chassis (13) are matched with the female thread (31) of funnel (3) by external screw thread (14).
The upper outside of described funnel (3) is provided with the vertical line (32) conveniently screwed, the inner surface (33) of funnel (3) is smooth, it is hemispherical, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with is easy to the band that funnel plug (4) is fixing.
The upper surface of described funnel plug (4) is sphere, the side of funnel plug (4) is provided with spring leaf (42), when funnel plug (4) fastens bell mouth (34) filling in funnel (3) bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3).
The thickness on described chassis (13) is 5～10mm, its diameter 20～50mm.
The internal diameter 4～9mm of described bell mouth (34)；Match with the inner cylinder face of bell mouth (34) in the face of cylinder of funnel plug (4).
Described funnel 3 is metal stainless steel material
Feature of the present utility model and having the beneficial effect that
(1) using electric mill to grind, the grind away time shortens, it is to avoid traditional grind away is lost time defect with manpower；(2) hand-held grind away is easy to operate, quick, safety, at least can improve the work efficiency 80% of testing crew；(3) unique leaf that flies designs, and drives when grind away sample powder to circulate with air, makes sample fully be smashed, and eliminates common grind away probability the most thoroughly；(4) confined space during grind away, the cross-contamination occurred when reducing common grind away, it is ensured that follow-up test result is more accurate, and confined space reduces the loss that sample flies away simultaneously；(5) mortar inner wall smooth, it is to avoid common grind away rear section sample is not easy the defect collected；(6) can directly put in liquid nitrogen with mortar interval in handle lower end, conduction hemisphere can ensure that and is in low temperature state inside mortar, this kind of grind away mode reduces the waste of liquid nitrogen, and isolation liquid nitrogen contacts with the direct of sample simultaneously, eliminates the danger of centrifuge tube blast；(7) one times grind away can be used for multiple times, avoid and commonly hang the defect that grind away of grinding machine is used only once, it is ensured that being smoothed out of test.
Accompanying drawing explanation
Fig. 1 is the main assembling schematic diagram of tissue sample milling machine；
Fig. 2 is the bottom assembling schematic diagram of tissue sample milling machine；
Fig. 3 is sample collection schematic diagram；
In figure, 1--grinding machine, 2--train line, 3--funnel, 4--funnel plug, 11--groove, 12--reinforcement, 13--chassis, 14--external screw thread, 15--rotary head, 31--female thread, 32--vertical line, 33--Internal Spherical Surface, 34--bell mouth, 4--funnel plug, the upper sphere of 41--, 42--spring leaf.
Detailed description of the invention
With embodiment, concrete structure of the present utility model is further illustrated below in conjunction with the accompanying drawings.
The main assembling structure of tissue sample milling machine is as shown in Figure 1, it is characterised in that, tissue sample milling machine is by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section 1.5 meters long train lines (2).
The bottom assembling structure of tissue sample milling machine is as shown in Figure 2, the groove (11) preventing hands from sliding it is provided with at the described handle hanging mill (1), hang mill (1) lower section and be provided with chassis (13), chassis (13) is reinforced by reinforcement (12) with hanging mill (1), and the outside of chassis (13) is provided with external screw thread (14)；The rotary head (15) bottom center from chassis (13) is stretched out；The upper inner of described funnel (3) is provided with female thread (31), and described chassis (13) are matched with the female thread (31) of funnel (3) by external screw thread (14)；The upper outside of described funnel (3) is provided with the vertical line (32) conveniently screwed, the inner surface (33) of funnel (3) is smooth, it is hemispherical, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with is easy to the band that funnel plug (4) is fixing；The upper surface of described funnel plug (4) is sphere, the side of funnel plug (4) is provided with spring leaf (42), when funnel plug (4) fastens bell mouth (34) filling in funnel (3) bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3)；The thickness on described chassis (13) is 5～10mm, its diameter 20～50mm；The internal diameter 4～9mm of described bell mouth (34)；Match with the inner cylinder face of bell mouth (34) in the face of cylinder of funnel plug (4)；Described funnel (3) is metal stainless steel material.
Use funnel plug (4) to fill in the bell mouth (34) of funnel (3) bottom, the sample good through liquid nitrogen freezing is put into the funnel (3) also passing through liquid nitrogen freezing and crossing；Being screwed by funnel (3) and be connected on chassis (13), power-on, sample is constantly smashed in rotary head (15).
Sample collection is as shown in Figure 3, after sample grind away, sample powder is swept to gently the side of funnel (3), take off funnel plug (4), bell mouth (34) is inserted into liquid nitrogen freezing good centrifuge tube (5) mouth, collects sample.Afterwards funnel (3) is partly screwed on chassis (13), take off funnel plug (4), tissue grinder is placed in ethanol, start power supply and clean dismembyator, take out tissue grinder afterwards, start power supply and dried or paper using wiped clean is used for testing next time.
The above; it is only this utility model preferably detailed description of the invention; but protection domain of the present utility model is not limited thereto; any those familiar with the art is in the technical scope that this utility model discloses; the change that can readily occur in or replacement, all should contain within protection domain of the present utility model.Therefore, protection domain of the present utility model should be as the criterion with scope of the claims.
Claims (3)
1. the sample milling machine being used for organizing grind away, it is characterised in that by hanging mill (1), train line (2), leakage
Bucket (3) funnel plug (4)；Hang mill (1) to be connected with motor by one section 1.5 meters long train lines (2)；
It is provided with the groove (11) preventing hands from sliding at the described handle hanging mill (1), hangs mill (1) lower section and arrange
Having chassis (13), chassis (13) are reinforced by reinforcement (12) with hanging mill (1), chassis (13)
Outside is provided with external screw thread (14)；The rotary head (15) bottom center from chassis (13) is stretched out；Described funnel (3)
Upper inner be provided with female thread (31), described chassis (13) are by external screw thread (14) and funnel (3)
Female thread (31) match；
The upper outside of funnel (3) is provided with the vertical line (32) conveniently screwed, the Internal Spherical Surface (33) of funnel (3)
Smooth, it is hemispherical, and the outer bottom of funnel (3) is provided with bell mouth (34), outside bell mouth (34)
Side is provided with is easy to the band that funnel plug (4) is fixing；The upper surface of described funnel plug (4) is sphere, funnel
The side of plug (4) is provided with spring leaf (42), fastens the leakage filling in funnel (3) bottom at funnel plug (4)
During quarrel (34), the upper sphere 41 of funnel plug (4) overlaps with the Internal Spherical Surface (33) of funnel (3).
The most according to claim 1 a kind of for organizing the sample milling machine of grind away, it is characterised in that chassis
(13) thickness is 5～10mm, its diameter 20～50mm.
The most according to claim 1 a kind of for organizing the sample milling machine of grind away, it is characterised in that funnel
The internal diameter 4～9mm of mouth (34)；The face of cylinder of funnel plug (4) matches with the inner cylinder face of bell mouth (34)
Close.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620341354.7U CN205599281U (en) | 2016-04-21 | 2016-04-21 | A grind appearance ware for organizing grind appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620341354.7U CN205599281U (en) | 2016-04-21 | 2016-04-21 | A grind appearance ware for organizing grind appearance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205599281U true CN205599281U (en) | 2016-09-28 |
Family
ID=56969723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620341354.7U Expired - Fee Related CN205599281U (en) | 2016-04-21 | 2016-04-21 | A grind appearance ware for organizing grind appearance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205599281U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105728145A (en) * | 2016-04-21 | 2016-07-06 | 广西作物遗传改良生物技术重点开放实验室 | Sample grinder for tissue sample grinding |
-
2016
- 2016-04-21 CN CN201620341354.7U patent/CN205599281U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105728145A (en) * | 2016-04-21 | 2016-07-06 | 广西作物遗传改良生物技术重点开放实验室 | Sample grinder for tissue sample grinding |
| CN105728145B (en) * | 2016-04-21 | 2018-01-26 | 广西作物遗传改良生物技术重点开放实验室 | A kind of sample milling machine for tissue grind away |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160928 Termination date: 20200421 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |