CN209193964U - Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution - Google Patents

Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution Download PDF

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Publication number
CN209193964U
CN209193964U CN201821610620.7U CN201821610620U CN209193964U CN 209193964 U CN209193964 U CN 209193964U CN 201821610620 U CN201821610620 U CN 201821610620U CN 209193964 U CN209193964 U CN 209193964U
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China
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culture solution
tube
cell culture
valve
test tube
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Expired - Fee Related
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CN201821610620.7U
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Chinese (zh)
Inventor
王勤
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Shanghai Xingjia Medical Technology Co Ltd
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Shanghai Xingjia Medical Technology Co Ltd
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Abstract

The utility model discloses a kind of placenta stem-cell culture solution extraction collecting devices of avoidable pollution, including support base and thermal-insulating body, the upper right side of the support base is fixedly connected with placement frame, the upper end of the test tube is provided with the first valve, the upper end of the rubber hose is communicated with connecting tube, the thermal-insulating body is located at the left side for placing frame, the inside of the thermal-insulating body is equipped with refrigerating plant, the upper right side of the storage container is provided with syringe tube, the inside of the syringe tube, which is run through, is connected with injector push-rod, cylinder shape valve is installed inside connecting tube between the syringe tube and the test tube of the leftmost side, it is fluted that connection is inlayed in the inside of the support base of the downside for placing frame.The placenta stem-cell culture solution that can avoid pollution, which extracts, uses collecting device, stores convenient for required culture solution is drawn into storage container as needed, this process avoids culture solution from contacting with air, to avoid polluting.

Description

Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution
Technical field
The utility model relates to placenta stem-cell culture solutions to extract correlative technology field, specially a kind of avoidable pollution Collecting device is used in the extraction of placenta stem-cell culture solution.
Background technique
Currently, stem cell is a kind of progenitor cell with self-replacation and Multidirectional Differentiation feature, stem cell is human body base This structural units and functional unit, it can thus be appreciated that stem cell is the cells of origin of the various histoorgans of human body, human body is from fertilization Egg development to fetus, from development of fetus to adult during the entire process of, stem cell loses its totipotency step by step, from all-round embryo Stem cell forms Subaerial blue green algae, multipotential stem cell and special energy stem cell, these are in the stem cell of different developmental phases Conclusive effect is played in the development of human tissue organ, growth, damage, repair process, it can be said that stem cell Body energy can be promoted vigorous, enable body keep fit state, stem cell is more demanding to condition of culture, and main component has: DMEM, EGF, serum, dual anti-, LIF, glutamine, 3-mercaptoethanol, nonessential amino acid etc..
But culture solution is not isolated with air when extracting culture solution by people, the impurity bacterium in air is held It easily breeds in culture solution, to influence the quality of culture solution, influences to placenta stem-cell culture, we have proposed one kind thus The placenta stem-cell culture solution that can avoid pollution, which extracts, uses collecting device, for solving the above problems.
Utility model content
It extracts the purpose of this utility model is to provide a kind of placenta stem-cell culture solution of avoidable pollution and is set with collection It is standby, it to solve general device mentioned above in the background art extracting culture solution is directly extracted by syringe, so The inside for re-injecting into collection device afterwards, readily accesses air, so that easy to carry impurity, causes culture solution to be contaminated Problem.
To achieve the above object, the utility model provides the following technical solutions: a kind of placenta stem-cell of avoidable pollution Culture solution, which extracts, uses collecting device, including support base and thermal-insulating body, and the upper right side of the support base, which is fixedly connected with, to be put The inside set frame, and place frame, which is run through, is connected with test tube, and the upper end of the test tube is provided with the first valve, and the first valve Inside through being connected with rubber hose, the upper end of the rubber hose is communicated with connecting tube, and rubber hose from top to bottom according to The secondary inside through the first valve and test tube, the thermal-insulating body are located at the left side for placing frame, and the fixed peace of thermal-insulating body Mounted in the left upper end of support base, the inside of the thermal-insulating body is equipped with refrigerating plant, and the upper end card slot of thermal-insulating body connects It is connected to storage container, the upper right side of the storage container is provided with syringe tube, and the lower end card slot of syringe tube is connected with card Block, and fixture block is through on the upper end inner wall of storage container, the inside of the syringe tube through being connected with injector push-rod, And the bottom righthand side of syringe tube is connected to the left end of connecting tube, in the connecting tube between the syringe tube and the test tube of the leftmost side Portion is equipped with cylinder shape valve, and the upper end of cylinder shape valve is connected with rotating block through connecting tube, and rotating block passes through cunning Rail is slidably connected on fixed block, and it is fluted that connection is inlayed in the inside of the support base of the downside for placing frame, and groove Inside through being connected with neonychium, and be intertwined and connected on the outside of neonychium and have spring.
Preferably, the placement frame is in "well" type frame structure, and places the equidistant card slot in inside of frame 4 groups of test tubes are connected with, and the height dimension of test tube is greater than the height dimension for placing frame.
Preferably, first valve includes sleeve, limiting slot and idler wheel, and the inside of sleeve is soft through rubber is connected with Pipe, sleeve are connected with idler wheel by limiting slot rolling.
Preferably, sleeve structure in a rectangular trapezoid, and the bevel edge of sleeve is arranged in parallel with limiting slot, and sleeve The spacing dimension of bevel edge and limiting slot is less than the radius size of idler wheel.
Preferably, the length dimension of the injector push-rod is less than the spacing of the left end of connecting tube and the upper end of syringe tube Size, and the cylinder shape valve inside connecting tube is 0-90 ° by the angular range that rotating block rotates on fixed block, and solid Determine angularly to be provided with arc-shaped sliding rail on block.
Preferably, the neonychium is symmetrical set about the central axes of groove, and the diameter dimension of groove is greater than examination The width dimensions of pipe.
Compared with prior art, the utility model has the beneficial effects that this can avoid the placenta stem-cell culture solution of pollution It extracts and uses collecting device, convenient for as needed storing the inside that required culture solution is drawn into storage container, this mistake Journey avoids culture solution from contacting with air, so that the problem of culture solution is contaminated is avoided,
1, it is equipped with the first valve, the idler wheel on the first valve is slided on sleeve by limiting slot, consequently facilitating adjusting The spacing between idler wheel and rubber hose is saved, when spacing increases, convenient for keeping the patency of rubber hose, on the contrary it is obstructed, Consequently facilitating extracting required culture solution from test tube as needed, collection is facilitated to use;
2, it is equipped with connecting tube and syringe tube, connecting tube is connected with syringe tube, consequently facilitating keeping extracting culture solution When, the contact with air is avoided, so that culture solution be avoided to be contaminated, influences the use of the culture to placenta stem-cell;
3, it is equipped with groove, neonychium and spring, when test tube is placed on the inside of groove, neonychium, which passes through, squeezes spring, Neonychium is shunk in the inside of groove, consequently facilitating the lower end to test tube is fixed, while being also convenient for utilizing spring There is elastic reaction certain elasticity to restore, and avoid excessive extrusion convenient for generating buffer function convenient for protecting to test tube, It influences to use.
Detailed description of the invention
Fig. 1 is that the utility model faces the schematic diagram of the section structure;
Fig. 2 is enlarged structure schematic diagram at A in the utility model Fig. 1;
Fig. 3 is the first valve mechanism of the utility model schematic diagram;
Fig. 4 is enlarged structure schematic diagram at B in the utility model Fig. 1;
Fig. 5 is the overlooking structure diagram of the utility model fixed block.
In figure: 1, support base;2, frame is placed;3, test tube;4, connecting tube;5, the first valve;501, sleeve;502, it limits Position slot;503, idler wheel;6, thermal-insulating body;7, refrigerating plant;8, storage container;9, syringe tube;10, fixture block;11, syringe pushes away Bar;12, rubber hose;13, groove;14, neonychium;15, spring;16, cylinder shape valve;17, fixed block;18, rotating block; 19, sliding rail.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-5 is please referred to, the utility model provides a kind of technical solution: a kind of placenta stem-cell culture of avoidable pollution Liquid, which extracts, uses collecting device, including support base 1, placement frame 2, test tube 3, connecting tube 4, the first valve 5, thermal-insulating body 6, system Device for cooling 7, storage container 8, syringe tube 9, fixture block 10, injector push-rod 11, rubber hose 12, groove 13, neonychium 14, bullet Spring 15, cylinder shape valve 16, fixed block 17, rotating block 18 and sliding rail 19, the upper right side of support base 1 are fixedly connected with placing frame Frame 2, and place the inside of frame 2 through being connected with test tube 3, the upper end of test tube 3 are provided with the first valve 5, and the first valve 5 Through being connected with rubber hose 12, the upper end of rubber hose 12 is communicated with connecting tube 4 for inside, and rubber hose 12 from top to bottom according to The secondary inside through the first valve 5 and test tube 3, thermal-insulating body 6 are located at the left side for placing frame 2, and the fixed peace of thermal-insulating body 6 Mounted in the left upper end of support base 1, the inside of thermal-insulating body 6 is equipped with refrigerating plant 7, and the upper end card slot of thermal-insulating body 6 connects It is connected to storage container 8, the upper right side of storage container 8 is provided with syringe tube 9, and the lower end card slot of syringe tube 9 is connected with card Block 10, and fixture block 10 is through on the upper end inner wall of storage container 8, the inside of syringe tube 9 are pushed away through being connected with syringe Bar 11, and the bottom righthand side of syringe tube 9 is connected to the left end of connecting tube 4, the company between syringe tube 9 and the test tube 3 of the leftmost side Cylinder shape valve 16 is installed, and the upper end of cylinder shape valve 16 is connected with rotating block 18 through connecting tube 4 inside adapter tube 4, and And rotating block 18 is slidably connected on fixed block 17 by sliding rail 19, the inside for placing the support base 1 of the downside of frame 2 is inlayed Connect fluted 13, and the inside of groove 13 is through being connected with neonychium 14, and the outside of neonychium 14 is intertwined and connected bullet Spring 15.
Placing frame 2 as shown in figure 1 is in "well" type frame structure, and the equidistant card slot in inside for placing frame 2 is connected with 4 Group test tube 3, and the height dimension of test tube 3 is greater than the height dimension for placing frame 2, convenient for placing different types of culture solution, Convenient to use, if first valve 5 includes sleeve 501, limiting slot 502 and idler wheel 503 in Fig. 1 and 3, the inside of sleeve 501 is run through It is connected with rubber hose 12, sleeve 501 is connected with idler wheel 503 by the rolling of limiting slot 502, convenient for adjusting opening for rubber hose 12 Logical and closure, consequently facilitating extracting the culture solution inside different test tubes 3 as needed;
The structure in a rectangular trapezoid of sleeve 501 as shown in figure 1, and the bevel edge of sleeve 501 is arranged in parallel with limiting slot 502, and The bevel edge of sleeve 501 and the spacing dimension of limiting slot 502 are less than the radius size of idler wheel 503, pass through limiting slot 502, sliding sleeve Idler wheel 503 on cylinder 501, when slide downward, idler wheel 503 squeezes rubber hose 12, to avoid rubber hose 12 unobstructed, upwards Convenient for keeping the unobstructed of rubber hose 12, consequently facilitating the case where adjusting the closing and unlatching of rubber hose 12, is easy to use, such as The length dimension of injector push-rod 11 is less than the spacing dimension of the left end of connecting tube 4 and the upper end of syringe tube 9 in Fig. 1,4 and 5, And the cylinder shape valve 16 inside connecting tube 4 is 0-90 ° by the angular range that rotating block 18 rotates on fixed block 17, and It is angularly provided with arc-shaped sliding rail 19 on fixed block 17, cylinder shape valve 16 is rotated by rotating block 18, convenient for adjusting circle The positional relationship of cylindrical valve body 16 and connecting tube 4, consequently facilitating connecting tube 4 is opened or closed, consequently facilitating culture solution is extracted, side Just it uses;
As neonychium 14 is symmetrical set about the central axes of groove 13 in Fig. 1 and 2, and the diameter dimension of groove 13 is big In the width dimensions of test tube 3, convenient for providing certain buffer function, consequently facilitating being also convenient for while test tube 3 is fixed Test tube 3 is protected.
Working principle: when can avoid the placenta stem-cell culture solution extraction collecting device of pollution using this, first will Idler wheel 503 on first valve 5 of the rightmost side keeps rubber hose 12 to exist by the upward sliding on sleeve 501 of limiting slot 502 The patency of the inside of first valve 5, and the lower end outside nesting of rubber hose 12 is equipped with the temperature biography of model pt100 Sensor, consequently facilitating being monitored in real time to the culture solution inside test tube 3, by master reference, convenient for transmitting the temperature to On computer display screen, just understand with temperature of the people to culture solution, then leads to the drive cylinder shape valve 16 of rotating block 18 It crosses sliding rail 19 and rotates 90 ° on fixed block 17, it is parallel with the inner wall of connecting tube 4 convenient for the largest face of cylinder shape valve 16, thus Convenient for keeping the patency of connecting tube 4, then injector push-rod 11 is pulled up, consequently facilitating passing through connecting tube 4 and rubber Culture solution inside the test tube 3 of the rightmost side is fetched into the inside of syringe tube 9 by hose 12, and rotating block 18 is then driven cylinder Shape valve body 16 rotates 90 ° by sliding rail 19 on fixed block 17, convenient for the largest face of cylinder shape valve 16 and the inner wall of connecting tube 4 Vertically, consequently facilitating closing connecting tube 4, then injector push-rod 11 is pressed down against, convenient for culture solution is expressed to storage container 8 Inside, then fixture block 10 is pulled up so that fixture block 10 and syringe tube 9 carry out card slot connection, same mode by other The culture solution of inside of test tube 3 be fetched into the inside of storage container 8, concentrate culture solution, mixed, it is dry convenient for culture placenta Cell during this, avoids contact of the culture solution with air, thus the phenomenon that avoiding culture solution from polluting, influence using, Then the power switch of refrigerating plant 7 is opened, refrigerating plant 7 cools down to storage container 8, convenient for keeping storage container 8 Activity, keep from heat damage, influences using here it is the placenta stem-cell culture solution extraction collecting devices of avoidable pollution The whole process used.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (6)

1. a kind of placenta stem-cell culture solution of avoidable pollution, which extracts, uses collecting device, including support base (1) and incubator Body (6), it is characterised in that: the upper right side of the support base (1), which is fixedly connected with, places frame (2), and places frame (2) Inside is through being connected with test tube (3), and the upper end of the test tube (3) is provided with the first valve (5), and the inside of the first valve (5) Through being connected with rubber hose (12), the upper end of the rubber hose (12) is communicated with connecting tube (4), and rubber hose (12) is certainly It sequentially passes through under above in the inside of the first valve (5) and test tube (3), the thermal-insulating body (6) is located at the left side for placing frame (2) Side, and thermal-insulating body (6) is fixedly mounted on the left upper end of support base (1), the inside of the thermal-insulating body (6) is equipped with refrigeration Device (7), and the upper end card slot of thermal-insulating body (6) is connected with storage container (8), the upper right side setting of the storage container (8) Have syringe tube (9), and the lower end card slot of syringe tube (9) is connected with fixture block (10), and fixture block (10) is through memory On the upper end inner wall of ware (8), the inside of the syringe tube (9), which is run through, to be connected with injector push-rod (11), and syringe tube (9) Bottom righthand side be connected to the left ends of connecting tube (4), the connecting tube (4) between the syringe tube (9) and the test tube (3) of the leftmost side Inside is equipped with cylinder shape valve (16), and the upper end of cylinder shape valve (16) is connected with rotating block (18) through connecting tube (4), And rotating block (18) is slidably connected on fixed block (17) by sliding rail (19), the support of the downside for placing frame (2) The inside of pedestal (1), which is inlayed, connects fluted (13), and the inside of groove (13) is through being connected with neonychium (14), and protects Being intertwined and connected on the outside of pad (14) has spring (15).
2. a kind of placenta stem-cell culture solution of avoidable pollution according to claim 1, which extracts, uses collecting device, special Sign is: the placement frame (2) is in "well" type frame structure, and the equidistant card slot in inside for placing frame (2) is connected with 4 Group test tube (3), and the height dimension of test tube (3) is greater than the height dimension for placing frame (2).
3. a kind of placenta stem-cell culture solution of avoidable pollution according to claim 1, which extracts, uses collecting device, special Sign is: first valve (5) includes sleeve (501), limiting slot (502) and idler wheel (503), and the inside of sleeve (501) is passed through It wears and is connected with rubber hose (12), sleeve (501) is rolled by limiting slot (502) and is connected with idler wheel (503).
4. a kind of placenta stem-cell culture solution of avoidable pollution according to claim 3, which extracts, uses collecting device, special Sign is: the sleeve (501) structure in a rectangular trapezoid, and the bevel edge of sleeve (501) is arranged in parallel with limiting slot (502), and And the bevel edge of sleeve (501) and the spacing dimension of limiting slot (502) are less than the radius size of idler wheel (503).
5. a kind of placenta stem-cell culture solution of avoidable pollution according to claim 1, which extracts, uses collecting device, special Sign is: the length dimension of the injector push-rod (11) is less than between the left end of connecting tube (4) and the upper end of syringe tube (9) Carpenters square cun, and the cylinder shape valve (16) of connecting tube (4) inside passes through the angle that rotating block (18) rotate on fixed block (17) Range is 0-90 °, and arc-shaped sliding rail (19) are angularly provided on fixed block (17).
6. a kind of placenta stem-cell culture solution of avoidable pollution according to claim 1, which extracts, uses collecting device, special Sign is: the neonychium (14) is symmetrical set about the central axes of groove (13), and the diameter dimension of groove (13) is big Width dimensions in test tube (3).
CN201821610620.7U 2018-09-30 2018-09-30 Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution Expired - Fee Related CN209193964U (en)

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CN201821610620.7U CN209193964U (en) 2018-09-30 2018-09-30 Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution

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CN201821610620.7U CN209193964U (en) 2018-09-30 2018-09-30 Collecting device is used in a kind of placenta stem-cell culture solution extraction of avoidable pollution

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113148456A (en) * 2021-03-11 2021-07-23 上海拜力生物科技有限公司 Human myocardial cell separation reagent storage equipment and method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113148456A (en) * 2021-03-11 2021-07-23 上海拜力生物科技有限公司 Human myocardial cell separation reagent storage equipment and method thereof

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Granted publication date: 20190802

Termination date: 20210930