CN105728145B - A kind of sample milling machine for tissue grind away - Google Patents
A kind of sample milling machine for tissue grind away Download PDFInfo
- Publication number
- CN105728145B CN105728145B CN201610252335.1A CN201610252335A CN105728145B CN 105728145 B CN105728145 B CN 105728145B CN 201610252335 A CN201610252335 A CN 201610252335A CN 105728145 B CN105728145 B CN 105728145B
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- funnel
- plug
- bell mouth
- mill
- chassis
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/0056—Other disintegrating devices or methods specially adapted for specific materials not otherwise provided for
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to biotech equipment field, more particularly to a kind of sample milling machine for being used for plant or animal tissue.By hanging mill (1), train line (2), funnel (3) funnel plug (4)；Mill (1) train line (2) is hung with motor to be connected；The chassis (13) below mill (1) is hung to be engaged by screw thread funnel (3)；The upper outside of funnel (3) is provided with vertical line (32), the Internal Spherical Surface (33) of funnel (3) is smooth, the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34) is provided with band；The upper surface of funnel plug (4) is sphere, side is provided with spring leaf (42), funnel plug (4) fastens the bell mouth (34) for filling in funnel (3) bottom, and the upper sphere 41 of funnel plug (4) overlaps with the Internal Spherical Surface (33) of funnel (3).Grind away of the present invention is thorough, the time is short, saves sample and liquid nitrogen, reduces the possibility of cross pollution, eliminates the danger of sample cell blast.
Description
The invention belongs to agricultural and biotech equipment field, more particularly to a kind of grind away for being used for plant or animal tissue
Device.
Background technology
Grind away is the single stepping frequently involved in the technical fields such as agricultural, is specifically included：Agricultural, biotechnology, pharmacy,
Gardening etc., with the pay attention to day by day that people are tested biotechnology, grind away experiment application is more and more extensive.In grind away experiment, group
The grind away knitted is one frequent and laborious operation of testing crew, such as in large quantities of extraction RNA (ribonucleic acid) or DNA (deoxidations
Ribonucleic acid) experiment in, it is necessary to which each experiment to each time point repeats tissue grind away.Tissue can easily and fast,
The grind away of health is directly connected to test process speed and final test effect.
The material of grind away experiment is mainly plant tissue and animal tissue, during which to ensure the activity of molecular substance in tissue
Or integrality, so need to use liquid nitrogen to keep sample to be in low-temperature condition, therefore to test operation during grind away is organized
It is particularly severe.
Following defect be present in tradition tissue grind away operation：(1) the grind away time is long and waste muscle power, general grind away mainly use
Mortar, if thoroughly ground, it is necessary to which 20~30min, animal tissue is typically thicker, the required grind away time can waste people more long
Power is also more；(2) sample waste is serious, typically needs to be continuously added liquid nitrogen during grind away, often evokes when adding
Dust flies away container, also has amount of powder in addition and remains in the inwall of sample milling machine and can not collect, less or precious for measuring
Sample, this waste are difficult to receive；(3) liquid nitrogen wastes serious, liquid nitrogen is typically continuously added in grind away, in container
Have and ground when liquid nitrogen be present, sample can flow everywhere with liquid nitrogen, so now the efficiency of grind away is low, only wait until that liquid nitrogen is soon waved
Hair grind away, therefore have substantial amounts of liquid nitrogen and slattern to the greatest extent；(4) grind away is not thorough, and general grind away comes often through the color of powder
Judge whether grind away is complete, but different tissues or the different times of different tissues just effectively can not directly judge, this
Kind of problem eliminates often by the increase grind away time, so so also causing defect 1 indirectly), 2) and 3), typically hang mill and grind
Sample device is the grind away in 1.5ml conical centrifuge tubes, and due to centrifuge tube and rotary head, sample can not thoroughly be ground, sample
Can not thoroughly it crush, may result in the material needed for follow-up test can not effectively extract；5) cross pollution be present, because grind away is
Carried out in the container of opening, so when adding liquid nitrogen, the powder evoked can fly away everywhere, dirty often through liquid nitrogen and container
Other test specimens are contaminated, the experiment strict for requiring comparison, such operation may result in test error；6) sample and liquid
The direct contact of nitrogen adds the risk of sample storage, and sample often carries part liquid nitrogen when being stored into centrifuge tube, works as temperature
To a certain extent, the liquid nitrogen in centrifuge tube can be changed into gas, can increase the pressure in centrifuge tube, so subsequently trying for rise
The risk of centrifuge tube blast often occurs in testing.
The content of the invention
The invention aims to solve, the common sample milling machine grind away time is long, it is physical to waste, wastes sample and liquid nitrogen, mill
Sample is not thorough and the problem of cross pollution, there is provided a kind of tissue sample milling machine easy to operate, time saving, hygienic.Characterized in that, group
Sample milling machine is knitted by hanging mill (1), train line (2), funnel (3) funnel plug (4)；Hang mill (1) and pass through one section of 1.5 meters of long train line
(2) it is connected with motor.
The groove (11) for preventing that rubber sleeve is dynamic is provided with the handle for hanging mill (1), hangs and is provided with chassis below mill (1)
(13), chassis (13) are reinforced with hanging mill (1) by reinforcement (12), and the outside of chassis (13) is provided with external screw thread (14)；Rotary head
(15) stretched out from the bottom center of chassis (13).
The upper inner of the funnel (3) is provided with internal thread (31), and the chassis (13) passes through external screw thread (14) and funnel
(3) internal thread (31) is engaged.
The upper outside of the funnel (3) is provided with the vertical line (32) conveniently screwed, and the inner surface (33) of funnel (3) is smooth,
It is hemispherical, and the outer bottom of funnel (3) is provided with bell mouth (34), and the outside of bell mouth (34), which is provided with, is easy to funnel plug (4) solid
Fixed band.
The upper surface of the funnel plug (4) is sphere, and the side of funnel plug (4) is provided with spring leaf (42), in funnel plug (4)
When fastening fills in bell mouth (34) of funnel (3) bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3).
The thickness of the chassis (13) is 5~10mm, 20~50mm of its diameter.
4~9mm of internal diameter of the bell mouth (34)；The face of cylinder of funnel plug (4) and the inner cylinder face phase of bell mouth (34)
Coordinate.
The funnel 3 is metal stainless steel material
The features of the present invention and have the beneficial effect that：
(1) ground using electric mill, the grind away time shortens, and avoids traditional grind away and loses time and the defects of manpower；(2) hand
Hold that grind away is easy to operate, quick, safe, can at least improve the operating efficiency 80% of testing crew；(3) unique winged leaf is set
Meter, sample powder is driven sample is fully smashed with air circulation, eliminate common grind away and not enough thoroughly may in grind away
Property；(4) confined space during grind away, the cross pollution occurred during common grind away is reduced, it is ensured that follow-up test result is more accurate
Really, while confined space reduces the loss that sample flies away；(5) mortar inner wall smooth, sample segment is not after avoiding common grind away
The defects of easily collecting；(6) directly can be put into mortar interval in liquid nitrogen handle lower end, conduction hemisphere can ensure mortar
Inside is in low-temperature condition, and such a grind away mode reduces the waste of liquid nitrogen, while completely cuts off liquid nitrogen and contacted with the direct of sample, disappears
Except the danger of centrifuge tube blast；(7) grind aways can be used for multiple times, avoid and common hang grind away of grinding machine and can only use
Time the defects of, it is ensured that experiment is smoothed out.
Brief description of the drawings
Fig. 1 is the main assembling schematic diagram of tissue sample milling machine；
Fig. 2 is the bottom assembling schematic diagram of tissue sample milling machine；
Fig. 3 is sample collection schematic diagram；
In figure, 1-- grinding machines, 2-- train lines, 3-- funnels, 4-- funnel plugs, 11-- grooves, 12-- reinforcements, 13-- bottoms
Disk, 14-- external screw threads, 15-- rotary heads, 31-- internal threads, 32-- vertical lines, 33-- Internal Spherical Surfaces, 34-- bell mouths, 4-- funnel plugs,
The upper spheres of 41--, 42-- spring leafs.
Embodiment
The concrete structure of the present invention is further illustrated with reference to the accompanying drawings and examples.
Organize the main assembling structure of sample milling machine as shown in Figure 1, it is characterised in that tissue sample milling machine is by hanging mill (1), train line
(2), funnel (3) funnel plug (4)；Mill (1) is hung by one section of 1.5 meters of long train line (2) with motor to be connected.
The bottom assembling structure of sample milling machine is organized as shown in Fig. 2 being provided with prevent rubber sleeve dynamic at the handle for hanging mill (1)
Groove (11), hang and chassis (13) is provided with below mill (1), chassis (13) pass through reinforcement (12) reinforcing, chassis with hanging mill (1)
(13) outside is provided with external screw thread (14)；Rotary head (15) is stretched out from the bottom center of chassis (13)；In the top of the funnel (3)
Side is provided with internal thread (31), and the chassis (13) is engaged by external screw thread (14) with the internal thread (31) of funnel (3)；The leakage
The upper outside of bucket (3) is provided with the vertical line (32) that conveniently screws, and the inner surface (33) of funnel (3) is smooth, and it is hemispherical, funnel
(3) outer bottom is provided with bell mouth (34), and the outside of bell mouth (34) is provided with the band for being easy to funnel plug (4) fixed；The leakage
The upper surface of bucket plug (4) is sphere, and the side of funnel plug (4) is provided with spring leaf (42), is fastened in funnel plug (4) and fills in funnel
(3) during bell mouth (34) of bottom, the upper sphere of funnel plug (4) overlaps with the inner surface (33) of funnel (3)；The chassis (13)
Thickness be 5~10mm, 20~50mm of its diameter；4~9mm of internal diameter of the bell mouth (34)；The face of cylinder of funnel plug (4) with
The inner cylinder face of bell mouth (34) is engaged；The funnel (3) is metal stainless steel material.
Filled in the bell mouth (34) of funnel (3) bottom, will be put into by the good sample of liquid nitrogen frozen using funnel plug (4)
Also pass through in the funnel (3) that liquid nitrogen frozen is crossed；Funnel (3) is screwed and is connected on chassis (13), power-on, rotary head (15)
Constantly smash sample.
Sample powder is gently swept to the side of funnel (3), removed by sample collection as shown in figure 3, after sample grind away
Funnel plug (4), bell mouth (34) is inserted into the good centrifuge tube of liquid nitrogen frozen (5) mouth, collects sample.Afterwards by funnel (3) half
It is screwed on chassis (13), removes funnel plug (4), tissue grinder is placed in alcohol, starts power supply cleaning mill, Zhi Houqu
Go out tissue grinder, start power supply and dried or be used to test next time with paper wiped clean.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in,
It should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with scope of the claims
It is defined.
Claims (1)
1. a kind of sample milling machine for tissue grind away, it is characterised in that by hanging mill（1）, train line（2）, funnel（3）, funnel plug
（4）Composition；Hang mill（1）Pass through one section of 1.5 meters of long train line（2）It is connected with motor；
It is described to hang mill（1）Handle at be provided with the groove for preventing rubber sleeve dynamic（11）, hang mill（1）Lower section is provided with chassis（13）, bottom
Disk（13）With hanging mill（1）Pass through reinforcement（12）Reinforce, chassis（13）Outside be provided with external screw thread（14）；Rotary head（15）From chassis
（13）Bottom center stretch out；The funnel（3）Upper inner be provided with internal thread（31）, the chassis（13）Pass through external screw thread
（14）With funnel（3）Internal thread（31）It is engaged；
Funnel（3）Upper outside be provided with the vertical line that conveniently screws（32）, funnel（3）Internal Spherical Surface（33）Smooth, it is hemisphere
Shape, funnel（3）Outer bottom be provided with bell mouth（34）, bell mouth（34）Outside be provided be easy to funnel plug（4）Fixed band；
The funnel plug（4）Upper surface be sphere, funnel plug（4）Side be provided with spring leaf（42）, in funnel plug（4）Fastening is filled in
Funnel（3）The bell mouth of bottom（34）When, funnel plug（4）Upper sphere 41 and funnel（3）Internal Spherical Surface（33）Overlap；
The chassis（13）Thickness be 5 ~ 10mm, its a diameter of 20 ~ 50mm；
The bell mouth（34）Internal diameter be 4 ~ 9mm；Funnel plug（4）The face of cylinder and bell mouth（34）Inner cylinder face match
Close.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252335.1A CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252335.1A CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105728145A CN105728145A (en) | 2016-07-06 |
| CN105728145B true CN105728145B (en) | 2018-01-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610252335.1A Active CN105728145B (en) | 2016-04-21 | 2016-04-21 | A kind of sample milling machine for tissue grind away |
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| Country | Link |
|---|---|
| CN (1) | CN105728145B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108855504A (en) * | 2018-06-11 | 2018-11-23 | 黄崇轩 | A kind of sample milling machine of use for laboratory tissue polishing |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109855932A (en) * | 2019-01-02 | 2019-06-07 | 中铝材料应用研究院有限公司 | A method of preparing rolling aluminium alloy sheet EBSD sample |
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| US4072275A (en) * | 1975-12-05 | 1978-02-07 | Mettler Instruments Ag | Sample preparation device |
| JPS63115438U (en) * | 1987-01-19 | 1988-07-25 | ||
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
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| CN204359699U (en) * | 2015-01-27 | 2015-05-27 | 于艳丽 | A kind of instrument for detecting pesticide residue through |
| CN204944925U (en) * | 2015-09-17 | 2016-01-06 | 四川农业大学 | A kind of refrigeration grinding machine |
| CN105251564A (en) * | 2015-11-19 | 2016-01-20 | 江西师范大学 | Biological sample freezing and grinding machine |
| CN205599281U (en) * | 2016-04-21 | 2016-09-28 | 广西作物遗传改良生物技术重点开放实验室 | A grind appearance ware for organizing grind appearance |
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Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1289121A (en) * | 1969-02-10 | 1972-09-13 | ||
| DE2260277A1 (en) * | 1972-11-17 | 1974-06-12 | Saiki | LAB ANALYSIS INSTRUMENTS |
| US4072275A (en) * | 1975-12-05 | 1978-02-07 | Mettler Instruments Ag | Sample preparation device |
| JPS63115438U (en) * | 1987-01-19 | 1988-07-25 | ||
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
| CN204286891U (en) * | 2014-11-24 | 2015-04-22 | 中国烟草总公司郑州烟草研究院 | One grows tobacco hard tissues cryogrinding device |
| CN204359699U (en) * | 2015-01-27 | 2015-05-27 | 于艳丽 | A kind of instrument for detecting pesticide residue through |
| CN204944925U (en) * | 2015-09-17 | 2016-01-06 | 四川农业大学 | A kind of refrigeration grinding machine |
| CN105251564A (en) * | 2015-11-19 | 2016-01-20 | 江西师范大学 | Biological sample freezing and grinding machine |
| CN205599281U (en) * | 2016-04-21 | 2016-09-28 | 广西作物遗传改良生物技术重点开放实验室 | A grind appearance ware for organizing grind appearance |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108855504A (en) * | 2018-06-11 | 2018-11-23 | 黄崇轩 | A kind of sample milling machine of use for laboratory tissue polishing |
| CN108855504B (en) * | 2018-06-11 | 2021-08-27 | 瑞安市华澳绿康农业科技有限公司 | Sample grinding device for tissue polishing in laboratory |
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| Publication number | Publication date |
|---|---|
| CN105728145A (en) | 2016-07-06 |
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| CB03 | Change of inventor or designer information |
Inventor after: Qu Junjie Inventor after: Yin Ling Inventor after: Huang Yu Inventor after: Lu Jiang Inventor after: Wei Yuanwen Inventor after: Guo Wenfeng Inventor after: Fang Hui Inventor before: Yin Ling Inventor before: Qu Junjie Inventor before: Huang Yu Inventor before: Lu Jiang Inventor before: Wei Yuanwen Inventor before: Guo Wenfeng Inventor before: Fang Hui |
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