CN205528832U - External perfusion system - Google Patents
External perfusion system Download PDFInfo
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- CN205528832U CN205528832U CN201620148617.2U CN201620148617U CN205528832U CN 205528832 U CN205528832 U CN 205528832U CN 201620148617 U CN201620148617 U CN 201620148617U CN 205528832 U CN205528832 U CN 205528832U
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- Prior art keywords
- perfusion
- perfusion cell
- vitro
- cell
- perfusion system
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Abstract
The utility model discloses an external perfusion system, it includes stock solution bottle, thermostatted water bath, peristaltic pump, perfusion cell, collecting pipe and a plurality of pipe its characterized in that, save the nutrient solution in the stock solution bottle, during the nutrient solution was submerged to the one end of first pipe, the other end passed through the input of peristaltic pump connection perfusion cell, places experimental material in the perfusion cell, and the output of perfusion cell is through the 2nd pipe connection collecting pipe, and the perfusion cell is placed in the thermostatted water bath, wherein: perfusion cell body is the tube -shape, and the front end has long and thin portion, and the tail end stopper has the rubber buffer, and the rubber buffer is inserted by the 2nd pipe connection syringe needle, and long and thin portion is through the first pipe of silicone tube sealing connection. The utility model discloses required part obtains in all can following the laboratory, easily obtains and be with low costs, simple structure, easy dismounting, equipment is at any time used at any time, sealing connection between each part, sealing performance is good.
Description
Technical field
This utility model relates in vitro tissue experimental detection device field, specifically a kind of In vitro perfusion system.
Background technology
Modern medicine and study of pharmacy relate to different medicine, compound and different experiment conditions more and carry out sequence inertia stimulation,
Being the common pattern of modern medicine and pharmacology research, the current sequence inertia experiment form using perfusion or directly dosing carries out more.Directly add
Medicine is uneven and easily cause accurate research unstable etc. shortcoming is many at present is not used because easily causing tested material.Perfusion experiment
Then need special perfusion system, it is desirable to whole perfusion pipeline is for sealing pipeline, and system cost is expensive.
Utility model content
This utility model is to solve problem present in background technology, proposes a kind of In vitro perfusion system, and it includes liquid containing bottle, perseverance
Warm water bath, peristaltic pump, perfusion cell, collecting pipe and some conduits, store nutritional solution in described liquid containing bottle, the first conduit
One end is submerged in nutritional solution, and the other end connects the input of perfusion cell by peristaltic pump, places experiment material in perfusion cell,
The outfan of perfusion cell connects collecting pipe by the second conduit, and perfusion cell is placed in thermostatical water bath, wherein: described filling
Stream cell body is tubular, and front end has elongate portion, and tail end is plugged with rubber stopper, and the second conduit connecting needle is inserted through rubber stopper, carefully
Long portion is tightly connected the first conduit by silica gel tube.
Preferably, perfusion system also includes that water proof container, perfusion cell are placed in thermostatical water bath by water proof container.
In a preferred embodiment, described water proof container is beaker.
In a preferred embodiment, described perfusion cell body is the syringe removed and advance head.
In a preferred embodiment, described rubber stopper is transversal section of the propelling of syringe.
Preferably, perfusion cell also includes hollow rubber pipe and 400 mesh nylon wires, and 400 mesh nylon wires are extruded by hollow rubber plug
It is fixed on the interior forward end of perfusion cell body.
In a preferred embodiment, described hollow rubber plug is transversal section of the propelling of syringe.
In a preferred embodiment, peristaltic pump is that multiple tracks is wriggled, and per pass can connect one group of first conduit, carries out organizing filling more simultaneously
Stream.
The beneficial effects of the utility model
Needed for this utility model, parts all can obtain from laboratory, it is easy to obtains and low cost；Simple in construction, easy accessibility,
Assemble at any time and use at any time；It is tightly connected between each parts, good seal performance.Multiple tracks peristaltic pump can support that organize perfusion enters more simultaneously
OK.
Accompanying drawing explanation
Fig. 1 is the first structured flowchart of this utility model.
Fig. 2 is this utility model the second structured flowchart.
Fig. 3 be the first structure of this utility model be preferable to carry out illustration.
Fig. 4 be this utility model the second structure be preferable to carry out illustration.
Fig. 5 be the first structure of this utility model perfusion cell be preferable to carry out illustration.
Fig. 6 be the second structure of this utility model perfusion cell be preferable to carry out illustration.
Detailed description of the invention
Below in conjunction with embodiment, the utility model is described in further detail, but protection domain of the present utility model is not limited to this:
Embodiment 1: combining Fig. 1 and Fig. 3, a kind of In vitro perfusion system, it includes liquid containing bottle 1, thermostatical water bath 4, wriggles
Pump 3, perfusion cell 5, collecting pipe 6 and some conduits, store nutritional solution 2, the one of the first conduit 7-1 in described liquid containing bottle 1
End submerges in nutritional solution 2, and the other end connects through peristaltic pump 3 and connects the input of perfusion cell 5, places in perfusion cell 5
Experiment material, the outfan of perfusion cell 5 connects collecting pipe 6 by the second conduit 7-2, and perfusion cell 5 is placed on thermostatted water
In bath 4.
Embodiment 2: combine Fig. 2 and Fig. 4, a kind of In vitro perfusion system, it also includes water proof container
8, perfusion cell 5 is placed in thermostatical water bath 4 by water proof container 8.In more excellent embodiment, water proof container 8 is for burning
Cup.
Embodiment 3: combine Fig. 5, a kind of In vitro perfusion system as described in embodiment 1 or 2, perfusion cell body 5-1 is
Tubular, front end (nutrient infusion end) has elongate portion 5-2, and tail end (nutritional solution outfan) is plugged with rubber stopper 5-3, the
Two conduit 7-2 connecting needle 5-4 are inserted through rubber stopper 5-3, and elongate portion 5-2 is tightly connected the first conduit 7-by silica gel tube 5-5
1。
Embodiment 4: a kind of In vitro perfusion system, perfusion cell body 5-1 is the note removed and advance head
Emitter (5ml).
Embodiment 5: a kind of In vitro perfusion system as described in embodiment 3 or 4, rubber stopper 5-3 is pushing away of syringe (5ml)
Enter transversal section.
Embodiment 6: combine Fig. 6, a kind of In vitro perfusion system as described in embodiment 3～5, perfusion cell 5 also includes hollow
400 mesh nylon wire 5-7 extruding are fixed on perfusion cell originally by rubber tube 5-6 and 400 mesh nylon wire 5-7, hollow rubber plug 5-6
The interior forward end of body 5-1.
Embodiment 7: a kind of In vitro perfusion system as described in embodiment 3～6, hollow rubber plug 5-6 is syringe (5ml)
Transversal section of propelling.
Embodiment 8: a kind of In vitro perfusion system as described in embodiment 3～7, peristaltic pump 3 is multiple tracks peristaltic pump, and per pass can connect
Meet a first conduit 7-1, carry out organizing perfusion simultaneously more.
This system not only has the feature of general isolated culture, and culture can be made to be in the environment of continuous renewal, eliminates
Products of cellular metabolism and the feedback effect of hormone, and culture can be made to accept the stimulation process of different frequency, intensity and time, suitable
Release dynamics in research hormone.
Nutritional solution 2 can use conventional nutritional solution, such as MEM, RPMI-1640, M199 etc..Thermostatical water bath 4 can use
Electric heating constant temperature three water tank, such as model S HH W21 600S etc..Peristaltic pump 3 can use the multiple tracks of GILSON to wriggle
Pump.
Use process: the experiment material aseptically taken out (as gastrointestinal tract mucous etc.) is with saline solution (such as PBS liquid)
Cleaning up, weigh, be placed in perfusion cell 5 and carry out hatching perfusion, perfusion rate can regulate voluntarily.During perfusion, liquid containing bottle
Nutritional solution 2 in 1 is provided power by modulated for synchronized peristaltic pump 3 respectively, enters by thermostatical water bath through the first conduit 7-1
The 4 perfusion cells 5 maintaining 37 DEG C of water-baths.After stable texture state, change nutritional solution 2 perfusion one section containing stimulating factor into
Time, then recover ordinary nutritional liquid 2 and (nutritional solution 2 can be recorded in advance from liquid containing bottle 1 to the time of perfusion cell 5, nutritional solution
2 flow out to the time of collecting pipe 6 from perfusion cell 5, in order to accurately experiment with computing process).By required time interval (
As every 5-10min collect once) in collecting pipe 6, collect perfusate ,-20 DEG C of sample preserves to be measured.
Specific embodiment described herein is only to illustrate this utility model spirit.Technology belonging to this utility model
Described specific embodiment can be made various amendment or supplements or use similar mode to replace by the technical staff in field
Generation, but without departing from spirit of the present utility model or surmount scope defined in appended claims.
Claims (8)
1. an In vitro perfusion system, it is characterised in that it includes liquid containing bottle, thermostatical water bath, peristaltic pump, perfusion cell, collecting pipe
And some conduits, described liquid containing bottle stores nutritional solution, one end of the first conduit is submerged in nutritional solution, and the other end is by wriggling
Pump connects the input of perfusion cell, places experiment material in perfusion cell, and the outfan of perfusion cell is by the second conduit even
Receiving collector, perfusion cell is placed in thermostatical water bath, wherein: described perfusion cell body is tubular, and front end has carefully
Long portion, tail end is plugged with rubber stopper, and the second conduit connecting needle is inserted through rubber stopper, and elongate portion is tightly connected first by silica gel tube
Conduit.
A kind of In vitro perfusion system the most according to claim 1, it is characterised in that perfusion system also includes water proof container, and perfusion is little
Room is placed in thermostatical water bath by water proof container.
A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described water proof container is beaker.
A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described perfusion cell body is to remove to advance head
Syringe.
A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described rubber stopper is that the propelling of syringe is transversal
Section.
A kind of In vitro perfusion system the most according to claim 1, it is characterised in that perfusion cell also includes hollow rubber pipe and 400
Mesh nylon wire, 400 mesh nylon wire extruding are fixed on the interior forward end of perfusion cell body by hollow rubber plug.
A kind of In vitro perfusion system the most according to claim 6, it is characterised in that described hollow rubber plug is the propelling head of syringe
Transversal section.
8. according to a kind of In vitro perfusion system described in any one of claim 1～7, it is characterised in that described peristaltic pump is multiple tracks peristaltic pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620148617.2U CN205528832U (en) | 2016-02-26 | 2016-02-26 | External perfusion system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620148617.2U CN205528832U (en) | 2016-02-26 | 2016-02-26 | External perfusion system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205528832U true CN205528832U (en) | 2016-08-31 |
Family
ID=56779375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620148617.2U Expired - Fee Related CN205528832U (en) | 2016-02-26 | 2016-02-26 | External perfusion system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205528832U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108917167A (en) * | 2018-08-07 | 2018-11-30 | 江苏斯托利仪器仪表有限公司 | A kind of constant temperature perfusion system |
| CN111212899A (en) * | 2017-10-16 | 2020-05-29 | 里珍纳龙药品有限公司 | Perfusion bioreactors and related methods of use |
-
2016
- 2016-02-26 CN CN201620148617.2U patent/CN205528832U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111212899A (en) * | 2017-10-16 | 2020-05-29 | 里珍纳龙药品有限公司 | Perfusion bioreactors and related methods of use |
| US11965153B2 (en) | 2017-10-16 | 2024-04-23 | Regeneron Pharmaceuticals, Inc. | Perfusion bioreactor and related methods of use |
| CN108917167A (en) * | 2018-08-07 | 2018-11-30 | 江苏斯托利仪器仪表有限公司 | A kind of constant temperature perfusion system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160831 Termination date: 20210226 |