CN205384407U - Reflective digital holographic microscope of dual wavelength - Google Patents
Reflective digital holographic microscope of dual wavelength Download PDFInfo
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- CN205384407U CN205384407U CN201620182264.8U CN201620182264U CN205384407U CN 205384407 U CN205384407 U CN 205384407U CN 201620182264 U CN201620182264 U CN 201620182264U CN 205384407 U CN205384407 U CN 205384407U
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Abstract
The utility model discloses a reflective digital holographic microscope of dual wavelength, including first laser instrument, second laser instrument, cube beam splitter prism, optical distance extender, first speculum, dispersion prism, cube beam combining prism and camera device, still include the second mirror, achromatic lens, achromatic microscope objective and sample platform. The utility model is used for the micro - formation of image of reflective the sample surface 3D, the light path of laser beam sharing and the optical element of two bundles of different wavelength for the device structure is simple relatively, and manufacturing cost is lower, and is simpler during the use, convenient to popularize and use.
Description
Technical field
This utility model relates to digital hologram technical field of imaging, is specifically related to a kind of dual wavelength reflective digital holographic microscope.
Background technology
Digital hologram (DigitalHolography), is the product that combines of common holography, computer technology, digital camera technology.The recording process of digital holography and common holography is essentially identical, the difference is that replacing holographic plate to record hologram with electro-photographic devices such as CCD, CMOS, and the hologram of record is stored in computer.Reproducing processes and the common holography of digital holography are also different, and it is, with numerical computation method, the hologram being stored in computer is carried out the reconstruction of hologram.Comparing with common holography, digital hologram has the advantage that
(1) owing to replacing holographic plate to record hologram with electronics picture pick-up device, required time of exposure can be very short, there is no the procedure for wet processing of chemical silver halide dry plate, each transients of moving object can be recorded continuously, it is achieved the continuous real-time online holographic recording under video；
(2) pass through digital reproduction, it is possible to obtain the intensity distributions of Object light wave and the quantitative image of phase mehtod simultaneously.
Digital holographic microscope is the product that Digital Holography and micro-imaging technique combine, it is possible to small items is carried out micro-imaging, has important application prospect in biomedical etc..The work process of existing digital holographic microscope is divided into two steps, and one is the hologram formed through optical interference with CCD or CMOS record, then hologram is stored in computer；Two is by computer simulated optical diffraction process, realizes being recorded the reconstruction of hologram of object by numerical computations.Passing through the reconstruction of hologram, it is possible to obtain the magnitude image and the position phase images that are recorded object simultaneously, and position phase images contains the three-dimensional information being recorded object, therefore great majority application is to utilize position phase images.
No matter the phase difference of two light waves owing to participating in interfering is how many, the position that reconstruction of hologram algorithm obtains be worth mutually be all betweenBetween, therefore exist be referred to as parcel position phase orThe mutually uncertain problem in position.In order to obtain the true position phase images being recorded object, it is necessary for carrying out position and solves parcel process mutually.But, due to discontinuous or sudden change appearance, in discontinuous or catastrophe point, position solves parcel mutually and there will be mistake.Parcel is solved mutually in order to more effectively discontinuous or catastrophe point be carried out position, dual wavelength Digital Holography is proposed, namely adopting and have the laser beam of two kinds of wavelength to record hologram, the parcel position phase images utilizing two kinds of wavelength corresponding respectively is combined and is carried out position and solve parcel mutually.
Dual wavelength Digital Holography, relative Single wavelength Digital Holography, on hologram recording apparatus, the problem that following two aspects will be considered: is to ensure that the same point of the same position pixel counter sample of the parcel position phase images that two kinds of wavelength are corresponding respectively；Two are to ensure that there is a larger angle in the direction of the holographic interference fringes that two kinds of wavelength are corresponding respectively so that in dual wavelength composite hologram Fourier spectrum, and the first component corresponding with two wavelength separates as far as possible.nullIn order to meet above-mentioned condition，Excessively complicated (the JonasK ü hn of the dual wavelength digital hologram recording equipment adopted at present,TristanColomb,FrédéricMontfort,FlorianCharrière,YvesEmery,EtienneCuche,PierreMarquet,andChristianDepeursinge.Real-timedual-wavelengthdigitalholographicmicroscopywithasinglehologramacquisition[J].OpticsExpress,2007,15 (12): 7231-7242.)，It is unfavorable for popularization and application.
Utility model content
The purpose of this utility model is the defect solving prior art, it is provided that a kind of dual wavelength reflective digital holographic microscope, and for reflective sample surfaces 3D micro-imaging, the technical scheme of employing is as follows:
A kind of dual wavelength reflective digital holographic microscope, including the first laser instrument, second laser, cube beam splitter prism, optical path lengthening device, the first reflecting mirror, dispersing prism, cube beam cementing prism and picture pick-up device, also include the second reflecting mirror, achromat, achromatic micro objective, sample stage and computer；Wherein, the first reflecting mirror, dispersing prism and the relative position of cube beam cementing prism make the direction of the holographic interference fringes that two kinds of wavelength are corresponding respectively inconsistent；First laser instrument exports the light beam that two bundle wavelength are different with second laser, two light beams are injected in cube beam splitter prism along the normal direction of cube beam splitter prism two not ipsilateral respectively, from the laser beam of a branch of dual wavelength mixing of the normal direction of the another two side of cube beam splitter prism each outgoing respectively；Wherein a branch of for reference beam, reference beam reflects into dispersing prism through optical path lengthening device, the first reflecting mirror, dispersing prism splits a beam into the two Single wavelength laser beams that the direction of propagation is slightly different, and two Single wavelength laser beams further rise injects one cube of beam cementing prism, then injects in picture pick-up device；Another bundle is object beam, object beam reflects into a cube beam cementing prism through the second reflecting mirror, the sample being placed on sample stage is irradiated then through achromat and achromatic micro objective, the light reflected by sample is injected in cube beam cementing prism through achromatic micro objective and achromat, sample is imaged onto interfering with reference beam as on sensitive face of picture pick-up device, forms dual wavelength composite hologram；After hologram is shot by picture pick-up device, it is stored in computer and carries out the reconstruction of hologram.
In this utility model, it is possible to adjust achromatic micro objective, achromat and sample stage along optical axis direction is suitably mobile, so that the image planes of sample are positioned on the picture sensitive face of picture pick-up device by achromatic micro objective and achromat；Optical path lengthening device is adjustable, makes reference beam and object beam arrive the optical path difference as sensitive face of picture pick-up device less than the coherence length of laser instrument.
As preferably, described first laser instrument and second laser setting spaced apart ninety degrees from.
As preferably, this utility model also includes the first spatial light wave filter and the first beam-expanding collimation lens that are set in turn between the first laser instrument and cube beam splitter prism, is set in turn in the second space optical filter between second laser and cube beam splitter prism and the second beam-expanding collimation lens.
The light beam that laser instrument is sent by spatial light wave filter and beam-expanding collimation lens respectively is filtered and beam-expanding collimation.
As preferably, the back focus of achromatic micro objective and the front focus of achromat overlap, and constitute a combinative optical system.
As preferably, achromat is plus lens.
Compared with prior art, the beneficial effects of the utility model:
This utility model is used for reflective sample surfaces 3D micro-imaging, and the laser beam of two bundle different wave lengths shares a light path and optical element so that apparatus structure is relatively easy, and manufacturing cost is lower, simpler during use, it is simple to promote the use of.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Detailed description of the invention
Below in conjunction with drawings and Examples, this utility model is described in further details.
nullEmbodiment: as shown in Figure 1，A kind of dual wavelength reflective digital holographic microscope，Including the first laser instrument 1、Second laser 2、Cube beam splitter prism 7、Optical path lengthening device 8、First reflecting mirror 9、Dispersing prism 10、Cube beam cementing prism 11 and picture pick-up device 17，Also include the second reflecting mirror 12、Achromat 13、Achromatic micro objective 14 and sample stage 16，Wherein，First reflecting mirror 9、Dispersing prism 10 makes the direction of the holographic interference fringes that two kinds of wavelength are corresponding respectively inconsistent with the relative position of cube beam cementing prism 11，First laser instrument 1 exports the light beam that two bundle wavelength are different with second laser 2，Two light beams are injected in cube beam splitter prism 7 along the normal direction of cube beam splitter prism 7 two not ipsilateral respectively，Laser beam from a branch of dual wavelength mixing of the normal direction of the another two side of cube beam splitter prism 7 each outgoing respectively，Wherein a branch of for reference beam，Reference beam is through optical path lengthening device 8、First reflecting mirror 9 reflects into dispersing prism 10，Dispersing prism 10 splits a beam into the two Single wavelength laser beams that the direction of propagation is slightly different，Two Single wavelength laser beams further rise injects one cube of beam cementing prism 11，Inject again in picture pick-up device 17；Another bundle is object beam, object beam reflects into cube beam cementing prism 11 through the second reflecting mirror, the sample 15 being placed on sample stage is irradiated then through achromat 13 and achromatic micro objective 14, the light reflected by sample is injected in cube beam cementing prism 11 through achromatic micro objective 14 and achromat 13, sample 15 is imaged onto interfering with reference beam as on sensitive face of picture pick-up device 17, forms dual wavelength composite hologram；After hologram is shot by picture pick-up device 17, it is stored in computer 18 and carries out the reconstruction of hologram.
In the present embodiment, it is possible to adjust achromatic micro objective 14, achromat 13 and sample stage 16 along optical axis direction is suitably mobile, so that the image planes of sample are positioned on the picture sensitive face of picture pick-up device 17 by achromatic micro objective 14 and achromat 13；Optical path lengthening device 8 is adjustable, makes reference beam and object beam arrive the optical path difference as sensitive face of picture pick-up device less than the coherence length of laser instrument.
Described first laser instrument 1 and second laser 2 setting spaced apart ninety degrees from.
The present embodiment also includes the first spatial light wave filter 3 and the first beam-expanding collimation lens 5 being set in turn between the first laser instrument 1 and cube beam splitter prism 7, is set in turn in the second space optical filter 4 between second laser 2 and cube beam splitter prism 7 and the second beam-expanding collimation lens 6.
The light beam that laser instrument is sent by spatial light wave filter and beam-expanding collimation lens respectively is filtered and beam-expanding collimation.
In the present embodiment, the back focus of achromatic micro objective 14 and the front focus of achromat 13 overlap, and constitute a combinative optical system.
In the present embodiment, achromat 13 is plus lens.
In the present embodiment, the first laser instrument 1 is the HONGGUANG helium neon laser of output to be 3mW, wavelength be λ=632.8nm；Second laser 2 is the gold-tinted helium neon laser of output to be 3mW, wavelength be λ=594nm；Spatial filter 3 and 4 is made up of pin hole and 20 x Microscope Objectives；Beam-expanding collimation lens 5 and 6 are K9 glass planoconvex lens that focal length is 60mm, diameter 30mm；Cube beam splitter prism 7 and cube beam cementing prism 11 adopt rib length to be 25.4mm, splitting ratio are the K9 glass cubic Amici prism of 1:1；Optical path lengthening device 8 is made up of the plane mirror of 4 K9 glass silverings, diameter 40mm；Dispersing prism 10 is 45 degree of equilateral prisms of 25.4mm, K9 glass for rib length；Reflecting mirror 9 and 12 is the plane mirror of K9 glass silvering, diameter 40mm；The achromatism plus lens 13 double; two glued achromatism plus lens of a diameter 25.4mm, focal length 60mm；Achromatic micro objective 14 uses apochromat；Picture pick-up device 17 adopts ccd video camera.
Claims (5)
1. a dual wavelength reflective digital holographic microscope, it is characterized in that, including the first laser instrument, second laser, cube beam splitter prism, optical path lengthening device, the first reflecting mirror, dispersing prism, cube beam cementing prism and picture pick-up device, also include the second reflecting mirror, achromat, achromatic micro objective, sample stage and computer；Wherein, the first reflecting mirror, dispersing prism and the relative position of cube beam cementing prism make the direction of the holographic interference fringes that two kinds of wavelength are corresponding respectively inconsistent；First laser instrument exports the light beam that two bundle wavelength are different with second laser, two light beams are injected in cube beam splitter prism along the normal direction of cube beam splitter prism two not ipsilateral respectively, from the laser beam of a branch of dual wavelength mixing of the normal direction of the another two side of cube beam splitter prism each outgoing respectively；Wherein a branch of for reference beam, reference beam reflects into dispersing prism through optical path lengthening device, the first reflecting mirror, dispersing prism splits a beam into the two Single wavelength laser beams that the direction of propagation is slightly different, and two Single wavelength laser beams further rise injects one cube of beam cementing prism, then injects in picture pick-up device；Another bundle is object beam, object beam reflects into a cube beam cementing prism through the second reflecting mirror, the sample being placed on sample stage is irradiated then through achromat and achromatic micro objective, the light reflected by sample is injected in cube beam cementing prism through achromatic micro objective and achromat, sample is imaged onto interfering with reference beam as on sensitive face of picture pick-up device, forms dual wavelength composite hologram；After hologram is shot by picture pick-up device, it is stored in computer and carries out the reconstruction of hologram.
2. a kind of dual wavelength reflective digital holographic microscope according to claim 1, it is characterised in that described first laser instrument and second laser setting spaced apart ninety degrees from.
3. a kind of dual wavelength reflective digital holographic microscope according to claim 1, it is characterized in that, also include the first spatial light wave filter and the first beam-expanding collimation lens that are set in turn between the first laser instrument and cube beam splitter prism, be set in turn in the second space optical filter between second laser and cube beam splitter prism and the second beam-expanding collimation lens.
4. a kind of dual wavelength reflective digital holographic microscope according to claim 1, it is characterised in that the back focus of achromatic micro objective and the front focus of achromat overlap, and constitute a combinative optical system.
5. a kind of dual wavelength reflective digital holographic microscope according to claim 1, it is characterised in that achromat is plus lens.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017181591A (en) * | 2016-03-28 | 2017-10-05 | 富士ゼロックス株式会社 | Digital holographic device |
| JP2017181580A (en) * | 2016-03-28 | 2017-10-05 | 富士ゼロックス株式会社 | Digital holographic device |
| CN107463081A (en) * | 2017-06-16 | 2017-12-12 | 重庆理工大学 | A kind of real-time micro-imaging detecting system of three-dimensional using Digital Holography |
| CN108007379A (en) * | 2017-11-20 | 2018-05-08 | 西北工业大学 | A kind of dual wavelength is total to railway digital holographic interferometry method and system |
| CN108415230A (en) * | 2018-05-15 | 2018-08-17 | 许之敏 | A kind of novel changable coke digital holographic microscope |
| CN109116708A (en) * | 2018-10-19 | 2019-01-01 | 许之敏 | A kind of dual wavelength reflective digital holographic microscope |
| CN111273534A (en) * | 2020-03-19 | 2020-06-12 | 嘉应学院 | Dual-wavelength digital holographic microscopic imaging method and device |
| CN112379583A (en) * | 2020-11-12 | 2021-02-19 | 昆明理工大学 | Lens-free system and color scanning holographic implementation method thereof |
| CN112985297A (en) * | 2021-02-07 | 2021-06-18 | 新余学院 | Reflection-type measurement-based dual-wavelength common-path digital holographic microscopic device and measurement method |
-
2016
- 2016-03-10 CN CN201620182264.8U patent/CN205384407U/en active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017181591A (en) * | 2016-03-28 | 2017-10-05 | 富士ゼロックス株式会社 | Digital holographic device |
| JP2017181580A (en) * | 2016-03-28 | 2017-10-05 | 富士ゼロックス株式会社 | Digital holographic device |
| CN107463081A (en) * | 2017-06-16 | 2017-12-12 | 重庆理工大学 | A kind of real-time micro-imaging detecting system of three-dimensional using Digital Holography |
| CN107463081B (en) * | 2017-06-16 | 2020-01-17 | 重庆理工大学 | Three-dimensional real-time microscopic imaging detection system using digital holographic technology |
| CN108007379A (en) * | 2017-11-20 | 2018-05-08 | 西北工业大学 | A kind of dual wavelength is total to railway digital holographic interferometry method and system |
| CN108415230A (en) * | 2018-05-15 | 2018-08-17 | 许之敏 | A kind of novel changable coke digital holographic microscope |
| CN109116708A (en) * | 2018-10-19 | 2019-01-01 | 许之敏 | A kind of dual wavelength reflective digital holographic microscope |
| CN111273534A (en) * | 2020-03-19 | 2020-06-12 | 嘉应学院 | Dual-wavelength digital holographic microscopic imaging method and device |
| CN111273534B (en) * | 2020-03-19 | 2021-05-04 | 嘉应学院 | Dual-wavelength digital holographic microscopic imaging method and device |
| CN112379583A (en) * | 2020-11-12 | 2021-02-19 | 昆明理工大学 | Lens-free system and color scanning holographic implementation method thereof |
| CN112985297A (en) * | 2021-02-07 | 2021-06-18 | 新余学院 | Reflection-type measurement-based dual-wavelength common-path digital holographic microscopic device and measurement method |
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