CN205329047U - Experiment equipment for exposing cells in nanoparticle aerosols - Google Patents
Experiment equipment for exposing cells in nanoparticle aerosols Download PDFInfo
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- CN205329047U CN205329047U CN201620108010.1U CN201620108010U CN205329047U CN 205329047 U CN205329047 U CN 205329047U CN 201620108010 U CN201620108010 U CN 201620108010U CN 205329047 U CN205329047 U CN 205329047U
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- aerosol
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- culture medium
- lamina membranacea
- board component
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Abstract
The utility model relates to an experiment equipment for exposing cells in nanoparticle aerosols, the on -line screen storage device comprises a base, be equipped with the spout on the base, be equipped with cell lamina membranacea subassembly on the spout, base upper end both sides are equipped with telescoping mast respectively, the subassembly is supplied with to the last aerosol that is equipped with of telescoping mast, the aerosol is supplied with the subassembly block and is in telescoping mast is last, cell lamina membranacea subassembly inboard is equipped with the cell lamina membranacea, and the outside is equipped with the culture medium export and the culture medium enters the mouth, culture medium export, culture medium entry with the cell lamina membranacea links to each other, the aerosol is supplied with the subassembly upper end and still is equipped with oxygen and carbon dioxide entry, the cell lamina membranacea subassembly outside still is equipped with gas collector, the utility model discloses beneficial effect provides safe, the convenient experimental facilities that exposes of cell nano particle aerosol that again can precise and quantitative control in the medical research for fields such as nano -material, nanometer toxicology, medicine, health and environmental protection.
Description
Technical field
This utility model relates to field of experiment equipment, and especially cell is exposed to the experimental facilities in nano granule aerosol。
Background technology
Along with nano material is in whole world extensive use, and then bring the particulate matter of nano material also day by day to highlight for biological potential hazard, research nano-particle specifically has for biology how the degree of which harm and harm is a global difficult problem, concern is progressively caused at academia for this, begin one's study and undertaken through respiratory tract, laboratory animal being carried out exposure experiment through skin, per os, collunarium by artificial analogy method, owing to nano material is small enough to permeates cell membranes easily, so more pressing close to。Exclusively carrying out, owing to there is no at present, ad hoc approach and the simulation experiment device that cell exposes in nano granule aerosol, similar nanometer of exposure test can only simply carry out laboratory animal is tested。Owing to the individual difference of animal and uncertain factor also have a strong impact on the accuracy of experiment, can not offer experimental data accurate, rigorous, simultaneously because do not have sealed environment, nano granule aerosol there is also certain infection risk, and serious meeting threatens the health of experimenter。Add animal welfare and protection aspect requires to adopt the cell replacement method more become more meticulous to resist animal experiment, thus constraining the development of association area research。Therefore design and develop experimental technique that a kind of cell is exposed in nano granule aerosol and equipment just becomes currently urgent needs。
Utility model content
In view of currently available technology above shortcomings, this utility model provides cell to be exposed to the experimental facilities in nano granule aerosol, it is possible to be exposed to the experiment in nano granule aerosol for cell。
For reaching above-mentioned purpose, of the present utility model adopt the following technical scheme that
Cell is exposed to the experimental facilities in nano granule aerosol, including base, described base is provided with chute, described chute is provided with cell membrane board component, described base upper end is respectively provided on two sides with telescopic column, described telescopic column is provided with aerosol feeding assembly, and described aerosol feeding assembly is fastened on described telescopic column；It is provided with cell lamina membranacea inside described cell membrane board component, outside is provided with culture medium outlet and culture medium entrance, the outlet of described culture medium, culture medium entrance are connected with described cell lamina membranacea, described aerosol feeding assembly is provided with aerosol entrance and aerosol spout, and described aerosol spout is connected with described aerosol entrance；Described aerosol feeding assembly upper end is additionally provided with oxygen and carbon dioxide entrance, is additionally provided with gas collector outside described cell membrane board component。
As optimal technical scheme of the present utility model, described aerosol feeding assembly is provided with sealing ring, for covering sealing with cell membrane board component。
As optimal technical scheme of the present utility model, in described cell membrane board component, it is provided with multiple described cell lamina membranacea。
As optimal technical scheme of the present utility model, described cell lamina membranacea is semipermeable membrane culture dish。
As optimal technical scheme of the present utility model, described cell membrane board component upper end is provided with multiple first engaging part, and described aerosol feeding assembly lower end is provided with multiple second engaging part, and described first engaging part is connected with described second engaging part。
As optimal technical scheme of the present utility model, described aerosol feeding assembly is additionally provided with a high-voltage generator。
As optimal technical scheme of the present utility model, in described cell membrane board component, it is additionally provided with a gas content sensor。
This utility model relates to the experimental facilities that cell is exposed in nano granule aerosol, including base, described base is provided with chute, described chute is provided with cell membrane board component, described base upper end is respectively provided on two sides with telescopic column, described telescopic column is provided with aerosol feeding assembly, and described aerosol feeding assembly is fastened on described telescopic column；Being provided with cell lamina membranacea inside described cell membrane board component, outside is provided with culture medium outlet and culture medium entrance, and the outlet of described culture medium, culture medium entrance are connected with described cell lamina membranacea；Described aerosol feeding assembly upper end is additionally provided with oxygen and carbon dioxide entrance; it is additionally provided with gas collector outside described cell membrane board component; this utility model provide the benefit that provide in medical research conveniently, safely again can accurate quantification control cell nano particulate aerosol expose experimental facilities, for fields such as nanomaterial science, nanotoxicology, medicine, health and environmental conservation。
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme in this utility model embodiment, the accompanying drawing used required in embodiment will be briefly described below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings。
Fig. 1 is structural representation of the present utility model。
Fig. 2 is the structural representation of aerosol feeding assembly in this utility model。
Fig. 3 is the structural representation of cell membrane board component in this utility model。
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is clearly and completely described, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole embodiments。Based on the embodiment in this utility model, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of this utility model protection。
As shown in Figure 1-Figure 3, this utility model provides cell to be exposed to the experimental facilities in nano granule aerosol, including base 1, base 1 is provided with chute 2, chute 2 is provided with cell membrane board component 3, this chute 2 is for the superincumbent slip of cell membrane board component 3, simple migratory cell lamina membranacea assembly 3 is controlled by guide rail, just can carry out cultivating the loading of position easily, conveniently it is operated in an experiment, base 1 upper end is respectively provided on two sides with telescopic column 4, telescopic column 4 is provided with aerosol feeding assembly 5, aerosol feeding assembly 5 is fastened on telescopic column 4, the raising and lowering of aerosol feeding assembly 5 can be realized by telescopic column 4, it is convenient for the operation of experiment；Being provided with cell lamina membranacea 6 inside cell membrane board component 3, outside is provided with culture medium outlet 7 and culture medium entrance 8, and culture medium outlet 7, culture medium entrance 8 are connected with cell lamina membranacea 6, it is possible to for input and the output of culture medium；Aerosol feeding assembly 5 upper end is additionally provided with oxygen and carbon dioxide entrance; to ensure cells survival environmental requirement; it is additionally provided with gas collector outside cell membrane board component 3; so that discharging safely after exposing the used smooth adsorption filtration of the gas containing nano-particle; this utility model provide the benefit that provide in medical research conveniently, safely again can accurate quantification control cell nano particulate aerosol expose experimental facilities, for fields such as nanomaterial science, nanotoxicology, medicine, health and environmental conservation。
In the present embodiment, also include embedded air flue, carry out gas exchange by embedded air flue；All component all can carry out autoclave sterilization；Agent structure of the present utility model is by senior silicate glass and polishing stainless steel manufacture, and profile is graceful and corrosion-resistant。
In the present embodiment, aerosol feeding assembly 5 is provided with sealing ring, for covering sealing with cell membrane board component 3。
In the present embodiment, multiple described cell lamina membranacea 6 it is provided with in cell membrane board component 3, it is preferably 3, three built-in culture chambers carry out independent culture medium supply respectively through bottom built-in pipe, medium flux can be controlled to adjust respectively by respective culture medium supply line, and by independent culture medium import and export, each culture chamber can carry out static state, intermittent or successional culture medium supply, and each culture chamber has independent Water jacket type circulation water-bath to control。
In the present embodiment, cell lamina membranacea 6 is semipermeable membrane culture dish。
In the present embodiment, cell membrane board component 3 upper end is provided with multiple first engaging part 9, aerosol feeding assembly 5 lower end is provided with multiple second engaging part 10, first engaging part 9 is connected with described second engaging part 10, that is the locking device that top structure passes through is cultivated exposure chamber easily and is tightly locked together with bottom, keeps good air-tightness。
In the present embodiment, aerosol feeding assembly 5 is additionally provided with a high-voltage generator。
In the present embodiment, being additionally provided with a gas content sensor in cell membrane board component 3 is, it is possible to for the oxygen content in cell membrane board component 3 is monitored。
In the present embodiment, experimental gas enters cell membrane board component 3 by the central intake pipe of top aerosol feeding assembly 5, then respectively enter exposure chamber respectively through three radial pipelines and carry out exposing contamination, and three internal chambeies that expose carry out independent exposure contamination respectively by independent air inlet/outlet。
In sum, this utility model relates to the experimental facilities that cell is exposed in nano granule aerosol, including base, described base is provided with chute, described chute is provided with cell membrane board component, described base upper end is respectively provided on two sides with telescopic column, and described telescopic column is provided with aerosol feeding assembly, and described aerosol feeding assembly is fastened on described telescopic column；Being provided with cell lamina membranacea inside described cell membrane board component, outside is provided with culture medium outlet and culture medium entrance, and the outlet of described culture medium, culture medium entrance are connected with described cell lamina membranacea；Described aerosol feeding assembly upper end is additionally provided with oxygen and carbon dioxide entrance; it is additionally provided with gas collector outside described cell membrane board component; this utility model provide the benefit that provide in medical research conveniently, safely again can accurate quantification control cell nano particulate aerosol expose experimental facilities, for fields such as nanomaterial science, nanotoxicology, medicine, health and environmental conservation。
The above; it is only detailed description of the invention of the present utility model; but protection domain of the present utility model is not limited thereto; any those skilled in the art is in technical scope disclosed in this utility model; the change that can readily occur in or replacement, all should be encompassed within protection domain of the present utility model。Therefore, protection domain of the present utility model should be as the criterion with described scope of the claims。
Claims (7)
1. cell is exposed to the experimental facilities in nano granule aerosol, it is characterized in that, including base, described base is provided with chute, described chute is provided with cell membrane board component, described base upper end is respectively provided on two sides with telescopic column, and described telescopic column is provided with aerosol feeding assembly, and described aerosol feeding assembly is fastened on described telescopic column；It is provided with cell lamina membranacea inside described cell membrane board component, outside is provided with culture medium outlet and culture medium entrance, the outlet of described culture medium, culture medium entrance are connected with described cell lamina membranacea, described aerosol feeding assembly is provided with aerosol entrance and aerosol spout, and described aerosol spout is connected with described aerosol entrance；Described aerosol feeding assembly upper end is additionally provided with oxygen and carbon dioxide entrance, is additionally provided with gas collector outside described cell membrane board component。
2. cell according to claim 1 is exposed to the experimental facilities in nano granule aerosol, it is characterised in that: described aerosol feeding assembly is provided with sealing ring, for covering sealing with cell membrane board component。
3. cell according to claim 1 is exposed to the experimental facilities in nano granule aerosol, it is characterised in that: it is provided with multiple described cell lamina membranacea in described cell membrane board component。
4. cell according to claim 1 is exposed to the experimental facilities in nano granule aerosol, it is characterised in that: described cell lamina membranacea is semipermeable membrane culture dish。
5. it is exposed to the experimental facilities in nano granule aerosol according to the arbitrary described cell of claim 1-4, it is characterized in that: described cell membrane board component upper end is provided with multiple first engaging part, described aerosol feeding assembly lower end is provided with multiple second engaging part, and described first engaging part is connected with described second engaging part。
6. cell according to claim 5 is exposed to the experimental facilities in nano granule aerosol, it is characterised in that: described aerosol feeding assembly is additionally provided with a high-voltage generator。
7. cell according to claim 5 is exposed to the experimental facilities in nano granule aerosol, it is characterised in that: it is additionally provided with a gas content sensor in described cell membrane board component。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620108010.1U CN205329047U (en) | 2016-01-28 | 2016-01-28 | Experiment equipment for exposing cells in nanoparticle aerosols |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620108010.1U CN205329047U (en) | 2016-01-28 | 2016-01-28 | Experiment equipment for exposing cells in nanoparticle aerosols |
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| Publication Number | Publication Date |
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| CN205329047U true CN205329047U (en) | 2016-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620108010.1U Expired - Fee Related CN205329047U (en) | 2016-01-28 | 2016-01-28 | Experiment equipment for exposing cells in nanoparticle aerosols |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110358671A (en) * | 2018-04-10 | 2019-10-22 | 北京慧荣和科技有限公司 | Multi link open-and-close mechanism |
| CN110804549A (en) * | 2019-11-20 | 2020-02-18 | 山东中医药大学附属医院 | Low-pressure and low-oxygen cell experimental device |
-
2016
- 2016-01-28 CN CN201620108010.1U patent/CN205329047U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110358671A (en) * | 2018-04-10 | 2019-10-22 | 北京慧荣和科技有限公司 | Multi link open-and-close mechanism |
| CN110804549A (en) * | 2019-11-20 | 2020-02-18 | 山东中医药大学附属医院 | Low-pressure and low-oxygen cell experimental device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160622 Termination date: 20170128 |
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| CF01 | Termination of patent right due to non-payment of annual fee |