CN205235991U - A micro -fluidic chip , detecting system for assembling phosphorus detects - Google Patents
A micro -fluidic chip , detecting system for assembling phosphorus detects Download PDFInfo
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- CN205235991U CN205235991U CN201521062984.2U CN201521062984U CN205235991U CN 205235991 U CN205235991 U CN 205235991U CN 201521062984 U CN201521062984 U CN 201521062984U CN 205235991 U CN205235991 U CN 205235991U
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Abstract
The application discloses a micro -fluidic chip, detecting system for assembling phosphorus detects, this chip include the colorimetric pool, communicate in the microchannel of colorimetric pool one end and communicate in the liquid outlet of the colorimetric pool other end, it has carrier liquid inlet, sample inlet, colouring agent inlet and reductant inlet to communicate in proper order in the liquid flow direction of microchannel edge, the colouring agent inlet with the intercommunication has the first mixed microchannel that promotes liquid mixing between the reductant inlet, the reductant inlet with the intercommunication has the mixed microchannel of second that promotes liquid mixing between the colorimetric pool, first mixed microchannel and the mixed microchannel of second are the passageway of the circuitous complications that form many times. The utility model discloses on -line measuring can be realized to the chip, and this micro -fluidic chip design configuration combines spectrophotometer to realize the content of the total phosphorus of on -line measuring in can inserting spectrophotometer's detection pond just.
Description
Technical field
The application belongs to water quality monitoring technical field, particularly relates to a kind of detect for total phosphorus micro-fluidicChip, detection system.
Background technology
Total phosphorus (TotalPhosphorus-TP) is one of three conventional indexs of water quality monitoring. Phosphorus is mainly depositedBe in industrial wastewater and sanitary sewage, the excessive meeting of total phosphorus causes the eutrophication of water body, follows algaeAbnormality proliferation, it rots to decompose and can consume the oxygen in water body, and then causes aquatile mortality, waterMatter worsens, and even causes the bogginess in lake.
It is in acid medium that macroscopic view detects the method for total phosphorus in water sample, will clear up completely orthophosphates andAmmonium molybdate reaction generates after phosphato-molybdic heteropolyacid under antimonic salt catalytic action, adds anti-sepsis acid to reduce,Generate blue complex, proceed to and in cuvette, carry out absorbance detection. Often complicated operation of this process, consumptionTime tediously long, and reagent consumption is large, has increased artificial and reagent cost, contaminated environment, and artificial disturbanceFactor is many, has reduced accuracy in detection.
Utility model content
The purpose of this utility model is to provide a kind of micro-fluidic chip, detection system detecting for total phosphorus,To overcome deficiency of the prior art.
For achieving the above object, the utility model provides following technical scheme:
The embodiment of the present application discloses a kind of micro-fluidic chip detecting for total phosphorus, comprises colorimetric pool, companyLead in the microchannel of colorimetric pool one end and be communicated in the liquid outlet of the colorimetric pool other end, described microchannelAlong being communicated with successively carrier fluid inlet, sample inlet, developer inlet on liquid flow direction and going backFormer dose of inlet, is communicated with between described developer inlet and described reducing agent inlet and promotes liquid mixedThe first mixing microchannel of closing, is communicated with promotion liquid between described reducing agent inlet and described colorimetric poolThe second mixing microchannel of mixing, described first mixes microchannel and second mixes microchannel for repeatedly roundaboutThe tortuous passage forming.
Preferably, at the above-mentioned micro-fluidic chip detecting for total phosphorus, described micro-fluidic chip comprisesUpper cover plate, lower cover and be formed at the chip channel plate between described upper cover plate and lower cover, described inColorimetric pool, microchannel are formed on described chip channel plate, offer respectively with described on described upper cover plateThe liquid injection hole that carrier fluid inlet, sample inlet, developer inlet and reducing agent inlet are communicated with, instituteState and on lower cover, offer the fluid hole being communicated with described liquid outlet.
Preferably, at the above-mentioned micro-fluidic chip detecting for total phosphorus, described chip channel plate comprisesMain part and convexedly stretch in the extension of main part one side, described microchannel is formed on described main part,Described colorimetric pool is formed on described extension.
Preferably, at the above-mentioned micro-fluidic chip detecting for total phosphorus, the passage of described colorimetric pool is recessedIf be formed at the upper surface of described main part, between described chip channel plate and upper cover plate, described in go outBetween liquid mouth and described colorimetric pool, be communicated with fluid pipeline, this fluid pipeline is arranged with that to be formed at described chip logicalThe lower surface of guidance tape, between described chip channel plate and lower cover, described in described liquid outlet is formed atOn main part.
Preferably, at the above-mentioned micro-fluidic chip detecting for total phosphorus, described upper cover plate, chip lead toThe material of guidance tape and lower cover is selected from glass, PC, PMMA or PDMS.
Preferably, at the above-mentioned micro-fluidic chip detecting for total phosphorus, described first mixes microchannelOr second mix the inwall of microchannel and protruded out lug boss, this lug boss accelerates to mix to the liquid of flowing throughClose.
Disclosed herein as well is a kind of total phosphorus detection system, comprising:
Described micro-fluidic chip;
Spectrophotometer, in contrastive colours pond, total phosphorus content detects.
Compared with prior art, the utility model has the advantage of:
(1), checkout gear of the present utility model is micro-fluidic chip, volume is little;
(2), the utility model chip can realize online detection, this micro-fluidic chip design configuration canJust insert in the detection cell of spectrophotometer, realized containing of online detection total phosphorus in conjunction with spectrophotometerAmount;
(3), application micro-fluidic chip reduced artificial disturbance factor, improved accuracy in detection.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present application or technical scheme of the prior art, below will be to realityThe accompanying drawing of executing required use in example or description of the Prior Art is briefly described, apparently, belowAccompanying drawing in description is only some embodiment that record in the application, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Figure 1 shows that the perspective view of micro-fluidic chip in the utility model specific embodiment;
Figure 2 shows that the utility model specific embodiment chips channel plate structural representation;
Figure 3 shows that upper cover plate structural representation in the utility model specific embodiment;
Figure 4 shows that lower cover structural representation in the utility model specific embodiment.
Detailed description of the invention
Micro-fluidic chip technology be a kind of in minute yardstick space the science and technology of fluid administration. ShouldWith microflow control technique by integrated the basic functions such as mixing, reaction and the detection core to tens square centimetersOn sheet, complete the detection to total phosphorus in water sample, compared with conventional method, there is high treatment effeciency, largeShorten greatly detection time; Less reagent consumption, has reduced sample cost, has reduced environmental pollution,Increase accuracy in detection. This detects significant for following total phosphorus.
Below in conjunction with the accompanying drawing in the utility model embodiment, to the technology in the utility model embodimentScheme is described in detail, and obviously, described embodiment is only that the utility model part is implementedExample, instead of whole embodiment. Based on the embodiment in the utility model, ordinary skill peopleThe every other embodiment that member obtains under the prerequisite of not making creative work, belongs to this practicalityNovel protected scope.
Shown in ginseng Fig. 1 to Fig. 4, the micro-fluidic chip detecting for total phosphorus, comprises upper cover plate 1, lower cover2 and be formed at the chip channel plate 3 between upper cover plate 1 and lower cover 2.
The thickness of upper cover plate and lower cover is preferably 1mm, and the thickness of chip channel plate is preferably 10mm.
On chip channel plate 3, be formed with colorimetric pool 301, be communicated in the microchannel 302 of colorimetric pool one end, withAnd being communicated in the liquid outlet 303 of the colorimetric pool other end, microchannel 302 is along being communicated with successively on liquid flow directionThere are carrier fluid inlet 304, sample inlet 305, developer inlet 306 and reducing agent inlet 307,Between developer inlet 306 and reducing agent inlet 307, be communicated with the first mixing that promotes that liquid mixesMicrochannel 308, is communicated with between reducing agent inlet 307 and colorimetric pool 301 and promotes second of liquid mixingMixing microchannel 309, the first mixes microchannels 308 and the second mixing microchannel 309 is repeatedly roundabout formationTortuous passage. On upper cover plate 1, offer respectively with carrier fluid inlet 304, sample inlet 305,The liquid injection hole 101 that developer inlet 306 and reducing agent inlet 307 are communicated with, offers on lower cover 2The fluid hole 201 being communicated with liquid outlet.
Microchannel is preferably dimensioned to be: wide 1mm, degree of depth 0.5mm.
In this technical scheme, carrier fluid inlet 304, sample inlet 305, developer inlet 306By dynamical system, liquid is injected respectively with reducing agent inlet 307, this dynamical system is micro-injectionPump, peristaltic pump, constant pressure pump or Miniature diaphragm pump.
In this technical scheme, the material of upper cover plate, chip channel plate and lower cover be selected from glass, PC,The high polymer material that PMMA or PDMS etc. are transparent, more preferably PMMA, employing high-temperature calcination,The method such as hot pressing or adhesive film by three layers of chip bonding together.
Further, chip channel plate 3 comprises main part 3010 and convexedly stretches in the extension of main part one sidePortion 3011, microchannel is formed on main part 3010, and colorimetric pool is formed on extension 3011.
Further, the passage of colorimetric pool is arranged with the upper surface that is formed at main part, is positioned at chip channelBetween plate and upper cover plate, between liquid outlet and described colorimetric pool, be communicated with fluid pipeline 3012, this drain pipeRoad 3012 is arranged with the lower surface that is formed at described chip channel plate, is positioned at described chip channel plate and lower coverBetween, liquid outlet 303 is formed on described main part.
Fluid pipeline is preferably dimensioned to be: wide 1mm, degree of depth 0.5mm.
In this technical scheme, the test section of detecting the micro-fluidic chip of total phosphorus is according to conventional light splitting lightThe detection cell of degree instrument designs, and in order to reduce reagent dosage, colorimetric pool width is 3mm, detects roadFootpath is 10mm. Whole chip is " T " type, be just inserted in the detection cell of spectrophotometer. ShouldThe colorimetric pool part of chip: intermediate layer is the groove of processing, is bonded together upper and lower cover plates and intermediate plate,Form closed pond body, and upper and lower surface is smooth. Adopt and make in this way colorimetric pool and can preventOn PMMA plate, directly cutting produces knife mark, affects accuracy in detection. The inlet of colorimetric pool is engraved in chipThe upper surface of channel plate, at the bottom of being positioned at colorimetric pool pond; Liquid outlet is engraved in the lower surface of chip channel plate, is positioned atThe Chi Ding of colorimetric pool, this design has effectively reduced the dead volume of colorimetric pool, prevents that solution is remaining, shortens steadyFix time, reduce error, reduce on the other hand chip volume, improve its utilization rate.
Further, the first inwall that mixes microchannel 308 or the second mixing microchannel 309 has protruded out protrudingPlay portion's (not shown), this lug boss accelerates to mix to the liquid of flowing through.
In this technical scheme, mix microchannel and adopt CNC machine tooling S type passage, by suitably subtractingThe speed of little fluid injection, the length that increases bend number and passage improves hybrid reaction efficiency. Mixed in order to improveClose reaction efficiency and can also in passage, do some special constructions, for example: pillar, rib shape or fish-boneThe lug bosses such as shape structure.
In this case, the chemical principle that in water quality, total phosphorus detects is: under acid condition, water sample is through disappearingThe orthophosphates obtaining after solution generates phosphomolybdic acid heteropoly acid with ammonium molybdate reaction under the catalysis of antimonic salt, shouldCompound is generated blue complex by ascorbic acid reduction, measures absorbance in wavelength 889nm place.
Utilize said chip to detect the method for total phosphorus, comprising:
(1), be first ready to four syringe pumps, syringe and micro-fluidic chip are connected, syringe is putPut on syringe pump, the extension of micro-fluidic chip is inserted in the slot that spectrophotometer is corresponding;
(2), open syringe pump, under its dynamic action, will clear up successively water sample, sulfuric acid carrier fluid, colour developingAgent and reducing agent are injected in micro-fluidic chip, wherein, by ammonium molybdate solution, antimony tartrate potassium solution withVolume ratio 3:1 mixes, and adds dissolution of sodium hydroxide to mix and is made into developer, and reducing agent is preferably ascorbic acidSolution;
(3) developer that, phosphorous water sample injects with developer inlet under the carrying of sulfuric acid carrier fluid is theOne mixes mixing in microchannel, reaction, generates phosphomolybdic acid heteropoly acid;
(4), phosphomolybdic acid heteropoly acid is reduced the reducing agent reduction generation blue complex that agent inlet injects;
(5), blue complex solution slowly flows in colorimetric pool, after colorimetric pool is full of, carry out continuouslyPower detects, and detects the absorbance of blue complex in 889nm place, taking the phosphatic concentration of standard as horizontalCoordinate, the absorbance of blue complex solution is ordinate Criterion curve, calculates according to calibration curveObtain the content of total phosphorus in water sample. Along with the continuous injection of solution, mixed solution flows out ratio from colorimetric pool topLook pond, enters fluid pipeline.
It should be noted that, in this article, the relational terms such as the first and second grades is only used forAn entity or operation are separated with another entity or operating space, and not necessarily require or implyBetween these entities or operation, there is relation or the order of any this reality. And term " comprises "," comprise " or its any other variant is intended to contain comprising of nonexcludability, thereby make to comprise that one isProcess, method, article or the equipment of row key element not only comprise those key elements, but also comprise do not have brightOther key elements of really listing, or it is intrinsic to be also included as this process, method, article or equipment instituteKey element. In the situation that there is no more restrictions, the key element being limited by statement " comprising ... ",And be not precluded within process, method, article or the equipment that comprises described key element, also exist other identicalKey element.
The above is only the application's detailed description of the invention, it should be pointed out that general for the artLogical technical staff, not departing under the prerequisite of the application's principle, can also make some improvement and profitDecorations, these improvements and modifications also should be considered as the application's protection domain.
Claims (7)
1. the micro-fluidic chip detecting for total phosphorus, is characterized in that, comprises colorimetric pool, is communicated inThe microchannel of colorimetric pool one end and be communicated in the liquid outlet of the colorimetric pool other end, described microchannel is along liquidOn body flow direction, be communicated with successively carrier fluid inlet, sample inlet, developer inlet and reducing agentInlet, is communicated with between described developer inlet and described reducing agent inlet and promotes what liquid mixedFirst mixes microchannel, is communicated with and promotes liquid to mix between described reducing agent inlet and described colorimetric poolSecond mix microchannel, described first mix microchannel and second mix microchannel be repeatedly roundabout formationTortuous passage.
2. the micro-fluidic chip detecting for total phosphorus according to claim 1, is characterized in that: instituteStating micro-fluidic chip comprises upper cover plate, lower cover and is formed between described upper cover plate and lower coverChip channel plate, described colorimetric pool, microchannel are formed on described chip channel plate, on described upper cover plateOffer respectively and described carrier fluid inlet, sample inlet, developer inlet and reducing agent inletThe liquid injection hole being communicated with, offers the fluid hole being communicated with described liquid outlet on described lower cover.
3. the micro-fluidic chip detecting for total phosphorus according to claim 2, is characterized in that: instituteState chip channel plate and comprise main part and convexedly stretch in the extension of main part one side, described microchannel formsOn described main part, described colorimetric pool is formed on described extension.
4. the micro-fluidic chip detecting for total phosphorus according to claim 3, is characterized in that: instituteThe passage of stating colorimetric pool is arranged with the upper surface that is formed at described main part, is positioned at described chip channel plate and upperBetween cover plate, between described liquid outlet and described colorimetric pool, be communicated with fluid pipeline, this fluid pipeline is arranged withBe formed at the lower surface of described chip channel plate, between described chip channel plate and lower cover, described inLiquid outlet is formed on described main part.
5. the micro-fluidic chip detecting for total phosphorus according to claim 2, is characterized in that: instituteThe material of stating upper cover plate, chip channel plate and lower cover is selected from glass, PC, PMMA or PDMS.
6. the micro-fluidic chip detecting for total phosphorus according to claim 1, is characterized in that: instituteThe inwall of stating the first mixing microchannel or the second mixing microchannel has protruded out lug boss, and this lug boss is to flowing throughLiquid accelerate mix.
7. a total phosphorus detection system, is characterized in that, comprising:
The arbitrary described micro-fluidic chip of claim 1 to 6;
Spectrophotometer, in contrastive colours pond, total phosphorus content detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521062984.2U CN205235991U (en) | 2015-12-18 | 2015-12-18 | A micro -fluidic chip , detecting system for assembling phosphorus detects |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201521062984.2U CN205235991U (en) | 2015-12-18 | 2015-12-18 | A micro -fluidic chip , detecting system for assembling phosphorus detects |
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| CN205235991U true CN205235991U (en) | 2016-05-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105562131A (en) * | 2015-12-18 | 2016-05-11 | 苏州汶颢芯片科技有限公司 | Micro-fluidic chip for total phosphorus detection, total phosphorus detection system and total phosphorus detection method |
| CN111175289A (en) * | 2018-11-09 | 2020-05-19 | 广州中国科学院先进技术研究所 | Multi-functional quick water quality testing system based on multispectral imaging and micro-fluidic technology |
-
2015
- 2015-12-18 CN CN201521062984.2U patent/CN205235991U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105562131A (en) * | 2015-12-18 | 2016-05-11 | 苏州汶颢芯片科技有限公司 | Micro-fluidic chip for total phosphorus detection, total phosphorus detection system and total phosphorus detection method |
| CN105562131B (en) * | 2015-12-18 | 2017-10-31 | 苏州汶颢芯片科技有限公司 | Micro-fluidic chip, detecting system and the detection method detected for total phosphorus |
| CN111175289A (en) * | 2018-11-09 | 2020-05-19 | 广州中国科学院先进技术研究所 | Multi-functional quick water quality testing system based on multispectral imaging and micro-fluidic technology |
| CN111175289B (en) * | 2018-11-09 | 2022-06-28 | 广州中国科学院先进技术研究所 | Multi-functional quick water quality testing system based on multispectral imaging and micro-fluidic technique |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 215000 A, 1 District, 128 Fang Zhou road, Suzhou Industrial Park, Jiangsu, Suzhou, China Patentee after: Suzhou Wen Hao microfluidic technology Limited by Share Ltd Address before: 215808 Suzhou Industrial Park, Jiangsu Road, No. 128 Patentee before: Suzhou Wenhao Chip Technology Co., Ltd. |
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| CP03 | Change of name, title or address |