CN205205150U - Electric shock pipe - Google Patents
Electric shock pipe Download PDFInfo
- Publication number
- CN205205150U CN205205150U CN201520981250.8U CN201520981250U CN205205150U CN 205205150 U CN205205150 U CN 205205150U CN 201520981250 U CN201520981250 U CN 201520981250U CN 205205150 U CN205205150 U CN 205205150U
- Authority
- CN
- China
- Prior art keywords
- chock plug
- electrode
- elastic component
- electric shock
- rod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Vibration Dampers (AREA)
Abstract
The utility model provides an electric shock pipe belongs to cell fusion equipment technical field. It has solved electrode among current electric shock pipe technical problem such as drop easily. This electric shock pipe includes body and chock plug, body inside has the cavity that is used for holding target liquid sample, the one end or the middle part of body are equipped with the first electrode that is linked together with the cavity, the other end of body has the opening that is linked together with the cavity, be equipped with the second electrode in the chock plug, the 2nd electrode package draws together the body of rod and the cap body, be equipped with the elastic component between the cap body and the chock plug, the body of rod insert establish in the chock plug and with chock plug sliding connection, inner and the cap body of the body of rod link firmly, the outer end of the body of rod can be through the opening and outside electric connection of chock plug, still be equipped with between second electrode and chock plug and prevent that the body of rod breaks away from the limit structure of chock plug, elastic component production compressive deformation makes the second electrode compress tightly on the opening when being fixed in the body tip with the chock plug. The utility model discloses in can prevent effectively through limit structure that the second electrode breaks away from the chock plug.
Description
Technical field
The utility model belongs to biological medicine plant and instrument technical field, relates to the electric shock pipe in a kind of cell electroporation equipment, particularly electroporation.
Background technology
Electroporation be utilize electricimpulse to introduce in cell cannot the macromolecular technology of permeates cell membranes, it is a kind of extensive employing and obtains the method for test cell line and gene therapy of strong preference.When applying highfield, cytolemma temporarily becomes porous character and can through foreign material, such as macromole.Cytolemma electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulsed voltage, pulse duration, number of pulses and other experiment conditions; Equipment at present for cell electroporation mainly contains cell electroporation, pole cup etc.
China patent CN1965079B discloses a kind of electroporation device with long hollow member, this electroporation device comprises long hollow member, to provide uniform electric field in electroporation process, wherein particularly, perform electroporation be by be full of in hollow member comprise cell and will inject cell the liquid sample of material after, apply electricimpulses by pair of electrodes from long hollow member two ends.
Applicant once declared one section of " method and apparatus of electroporation " (METHODSANDDEVICESFORELECTROPORATION by name; US2013/0052711A1) United States Patent (USP), a kind of sampling receptacle is described in this patent, here claim with the pipe belt that shocks by electricity, electric shock manages the sampling receptacle be namely equivalent in aforesaid U.S. Patent application, its effect adds containing cell and the liquid sample of material that will inject cell in pipe, at the upper/lower terminal of electric shock pipe, top electrode and lower electrode are set respectively, be connected with lower electrode with top electrode by cell electroporation, thus make to form electric field in electric shock pipe, and then extracellular material is injected in cell.After filling liquid sample in electric shock pipe, sample shape is fixed, and can not be formed bending between electrode by fluid surface as traditional open pole cup, eliminate the liquid internal electric field after liquid curved surface more even, can improve electroporation efficiency.And liquid sample being injected after in electric shock pipe, before starting electric shock, need in electric shock pipe to have prevented air residual from forming aeration distribution of current.In that patent, applicant is designed with an annular recesses be connected with electric shock tube chamber on the tube wall of electric shock pipe, experimenter is sustainable to injection liquid sample body in electric shock tube chamber until after fluid surface protrusion electric shock tube chamber, to contact until be pressed onto the upper edge of the tube chamber that shocks by electricity under the polar cap that power on liquid convex surface again, form the sealing to liquid inside cavity, and the liquid overflowed on a small quantity flows in annular recesses.This design generally can prevent having residual air to affect experiment in electric shock pipe.
But applicant finds in constantly putting into practice, the electric shock pipe in above-mentioned patent is higher to the precise requirements manufactured, and the requirement of experimenter's operation is simultaneously also higher.If accurate not in manufacture, easily cause the poor sealing on edge on electrode and cavity, may under electrode cap after generating electrodes upwarp and again leave edge on cavity and cause extraneous air to reenter the situation of cavity.If also easily cause there is residual air bubble in pipe in addition when operator's improper use, affect the effect of cell electroporation.Scientific experiment is very high to the reliability requirement of plant and instrument, and therefore, above-mentioned electric shock pipe needs on manufacturing, to increase some designs improving reliability to improve conventional efficient and the experiment effect that cell electricity melts test.
Summary of the invention
The utility model is the problems referred to above existed for existing technology, provides a kind of electric shock pipe.Technical problem to be solved in the utility model is: ensureing electric shock composite pipe tightness while, how to improve the stability that the second electrode is connected with chock plug.
The purpose of this utility model realizes by following technical proposal:
A kind of electric shock pipe, described electric shock pipe comprises body and chock plug, described tubular body has the cavity for holding target liq sample, one end of described body or middle part are provided with the first electrode be connected with cavity, the other end of described body has the opening be connected with described cavity, the second electrode is provided with in described chock plug, described second electrode comprises the body of rod and cap body, one end face of described cap body can fit with the annular end face of edge of opening, elastic component is provided with between the other end of described cap body and described chock plug, it is characterized in that, the described body of rod to be plugged in described chock plug and to be slidably connected with described chock plug, the inner and the described cap body of the described body of rod are connected, the outer end of the described body of rod can be electrically connected by the opening of described chock plug and outside, the position limiting structure that can prevent the body of rod from departing from chock plug is also provided with between described second electrode and chock plug, when chock plug being fixed on body end, described elastic component produces compressive set the second electrode is pressed on opening.
Its principle of work is as follows: this electric shock pipe in use, in cavity, the liquid sample of the material comprising cell and cell will be injected is filled in from the opening of body, then chock plug is fixed on body end and the annular end face at the external end face of the cap of the second electrode and body open edge is fitted, one end of first electrode and the second electrode all with liquid communication in cavity, the other end of the first electrode and the second electrode can electrically connect with outside, first electrode is connected with the pulse power with the second electrode, electric discharge makes form electric field in the cavity of electric shock pipe and make cytolemma have certain permeability, thus the target substance in liquid sample is entered in cell.In the technical program, when chock plug is fixed on body end, elastic component is between chock plug and the cap body of the second electrode, can be formed between cap body and chock plug and seal, and the body of rod of the second electrode is plugged in chock plug and be slidably connected with chock plug, can effectively prevent the second electrode from departing from chock plug by position limiting structure.
In above-mentioned electric shock pipe, described position limiting structure comprises the convex edge that described body of rod outer end extends along body of rod radial direction, and described convex edge radial dimension is more bigger than the bore of described chock plug opening; Described convex edge can extrude described chock plug under external force and produce deformation and the opening passing described chock plug.Chock plug can be passed smoothly in convex edge after external force extrusion plug capitiform becomes, and after chock plug recovers deformation, convex edge can keep spacing with chock plug, effectively prevents coming off of the second electrode.
In above-mentioned electric shock pipe, the opening part of described chock plug has the abutment face leant with described convex edge.Matching with abutment face through chock plug smoothly and keep spacing in convex edge after external force extrusion plug capitiform becomes, prevents the second electrode delamination.
In above-mentioned electric shock pipe, described convex edge is tapered, and the outer side of described convex edge has by the spigot surface one of the end face of convex edge face tilt extension laterally.By the guide effect of spigot surface one, the installation of the second electrode can be facilitated to place.
In above-mentioned electric shock pipe, described chock plug comprises the tubular portion with through hole one, the described body of rod is plugged in described through hole one, described position limiting structure comprises the annular protrusion on tubular portion inner side-wall, in the form of annular discs and the external diameter that is described cap body of described cap body is greater than the internal diameter of described annular protrusion, and described cap body can make the outer face of cap body be positioned on the upper side of annular protrusion through described annular protrusion.As another kind of position limiting structure scheme, can smoothly through the endoporus of annular projection after the annular protrusion deformation of cap body in external force extruded tubular part, then annular projection recovers deformation, the outer face of cap body and the upper side of annular protrusion lean and keep spacing, effectively prevent coming off of the second electrode.
In above-mentioned electric shock pipe, the height of the tubular portion of described chock plug is greater than the height of described elastic component.Such elastic component is positioned at tubular portion, and the cap body of the second electrode is also positioned at tubular portion inside, plays spacing effect, further by tubular portion to the second electrode and elastic component, the cap body of the second electrode and the inwall of tubular portion can also be made to be formed seal, thus improve stopping property.
In above-mentioned electric shock pipe, mount pad is in a tubular form provided with in tubular portion, the second above-mentioned electrode is slidably connected on mount pad, described elastic component is set on described mount pad, the height of described elastic component is greater than the height of mount pad, when chock plug being fixed on body end described elastic component produce compressive set make the upper surface of elastic component and chock plug against, the lower surface of elastic component and the upper surface of described cap body against.Elastic component between the tubular portion and mount pad of chock plug and also the two ends of elastic component lean with chock plug and the second electrode respectively, elastic component produces compressive set, elastic component and chock plug and the second electrode are formed seal, can avoid occurring gap between the second electrode and the opening of body, air is avoided to enter liquid sample in cavity, and when slightly upwarping appears in chock plug time, elastic component also can do certain recovery deformation, ensure that the second electrode still reclines mutually with the end face of edge of opening, thus make the liquid sample in cavity still can not produce air bubble when experimenter's application of sample operates some deviation, in sum, the technical program improves the sealing property between the second electrode and opening effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity.
Because elastic component is between the tubular portion and mount pad of chock plug, upper and lower two ends lean with chock plug and the second electrode again respectively, therefore elastic component there will not be the phenomenon come off, and elastic component can carry out spacing to the cap body of the second electrode simultaneously, prevents the excessively movement of the second electrode.
Body in the technical program, chock plug all adopt insulating material to make, and the first electrode and the second electrode adopt electro-conductive material to make, and this part is prior art, and not in this to go forth for the material of embody rule.In addition, the first electrode both can directly be fixed in body, also equally with the second electrode can be arranged on inside a chock plug and seal the cavity of body.
In above-mentioned electric shock pipe, the inner side-wall of described mount pad has the spigot surface three extended by the inside sidewall slope of the end face of described mount pad.By the guide effect of spigot surface three, the installation that can further facilitate the second electrode is placed.
In above-mentioned electric shock pipe, between the inner side-wall of the outer side of described elastic component and the tubular portion of described chock plug, there is gap.This gap of indwelling can provide certain space for the deformation of elastic component, thus utilizes the restorer of elastic component to realize the automatic adjustment carrying out shutoff gap between chock plug and the second electrode.
In above-mentioned electric shock pipe, described chock plug comprises the tubular portion with through hole one, the described body of rod is plugged in described through hole one, mount pad is in a tubular form provided with in described tubular portion, the second above-mentioned electrode is slidably connected on mount pad, described elastic component is the elastic part of described mount pad lower end, the described body of rod has the slope leant with described elastic component end face with the junction of cap body.The body of rod has slope and is used for inserting mount pad, and slope makes the elastic component generation elastic deformation on mount pad after inserting mount pad, improve stopping property.
In above-mentioned electric shock pipe, the periphery of described opening has can be fixed on body end by chock plug and make described elastic component produce the locating structure of compressive set.Make resilient member deforms can improve stopping property further by locating structure, also can effectively prevent chock plug from coming off from body end simultaneously.
In above-mentioned electric shock pipe, described locating structure comprises the connecting cylinder portion be connected as a single entity with the end of described body, described connecting cylinder portion offers the cylinder chamber for described chock plug grafting, the chamber wall in described cylinder chamber has convex tendon one, the outer side of described chock plug has can with the convex tendon two of described convex tendon one phase clamping.Convex tendon one and convex tendon two can be complete annular or discontinuous annular is not even annular, can reach the effect of clamping.Convex tendon one can not be clearly with the protrusion of convex tendon two, and the size adopting chock plug to fill in body portion is a bit larger tham the size inside connecting cylinder portion, reaches the object of location in an interference fit; Chock plug and the phase clamping of connecting cylinder portion, both are connected and is separated all very convenient, improve fluid injection and move the convenience of liquid after completing electric shock, certainly also can adopt the mode be threaded, when adopting screw thread, chock plug rotation can make it be fixed on connecting cylinder portion.
In above-mentioned electric shock pipe, alternatively, described locating structure comprises the grab one be arranged on described chock plug, described body has can with the buckle one of described grab one phase clamping.
In above-mentioned electric shock pipe, the end face of described edge of opening offers annular recesses, chock plug is connected by a flexible connecting member with described body.The two ends of flexible connecting member are connected with body with chock plug respectively, chock plug was both connected with body, relatively can swings by body again, avoiding because losing chock plug because of carelessness, the plastic thickness of described flexible piece makes smaller, can realize the flexibility that its wide-angle is bending.Chock plug can not be interfered like this to take off from body, can prevent again chock plug to drop loss, very easy to use.
Compared with prior art, the utility model has the following advantages:
1, in the technical program, the body of rod of the second electrode to be plugged in chock plug and to be slidably connected with chock plug, can effectively prevent the second electrode from departing from chock plug by position limiting structure.
2, in the technical program when chock plug is fixed on body end, elastic component produces compressive set and elastic component and chock plug and the second electrode is formed seal, can avoid occurring gap between the second electrode and the opening of body, air is avoided to enter liquid sample in cavity, and when slightly upwarping appears in chock plug time, elastic component also can do certain recovery deformation, ensure that the second electrode still reclines mutually with the end face of edge of opening, thus make the liquid sample in cavity still can not produce air bubble when experimenter's application of sample operates some deviation; Effectively improve the sealing property between the second electrode and opening, thus avoid the air in the external world when adding liquid sample to enter in cavity.
Accompanying drawing explanation
Fig. 1 is the perspective view of this electric shock pipe in embodiment one.
Fig. 2 is the vertical sectional structure schematic diagram of electric shock pipe in Fig. 1.
Fig. 3 is that in embodiment one, the enlarged partial sectional view after the second electrode installed by this electric shock pipe.
Fig. 4 is that in embodiment two, the enlarged partial sectional view after the second electrode installed by this electric shock pipe.
Fig. 5 is that in embodiment three, the enlarged partial sectional view after the second electrode installed by this electric shock pipe.
In figure, 1, body; 1a, opening; 1b, connecting cylinder portion; 1c, cylinder chamber; 1d, convex tendon one; 1e, cavity; 1f, annular recesses; 2, the first electrode; 3, the second electrode; 31, the body of rod; 31a, convex edge; 31b, spigot surface one; 31c, slope; 32, cap body; 4, chock plug; 41, through hole one; 43, convex tendon two; 44, mount pad; 45, tubular portion; 45a, spigot surface three; 45b, annular protrusion; 46, abutment face; 5, elastic component; 30, flexible connecting member.
Embodiment
Be below specific embodiment of the utility model and by reference to the accompanying drawings, the technical solution of the utility model is further described, but the utility model be not limited to these embodiments.
Embodiment one:
As Fig. 1, shown in Fig. 2 and Fig. 3, this electric shock pipe comprises body 1 and chock plug 4, chock plug 4 is connected on body 1 by flexible connecting member 30, body 1 inside has the cavity 1e for holding target liq sample, one end of body 1 or middle part are provided with the first electrode 2 be connected with cavity 1e, the other end of body 1 has the opening 1a be connected with cavity 1e, the end face at opening 1a edge offers annular recesses 1f, the second electrode 3 is provided with and the outer end of the second electrode 3 can be electrically connected by the opening of chock plug 4 and outside in chock plug 4, second electrode 3 is slidably connected with chock plug 4, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, it is characterized in that, chock plug 4 comprises the tubular portion 45 with through hole 1 and the mount pad 44 being in a tubular form positioned at tubular portion 45, the second above-mentioned electrode 3 to be slidably connected on mount pad 44 and to have the elastic component 5 of annular at mount pad 44 overcoat, the height of elastic component 5 is greater than the height of mount pad 44, the height of the tubular portion 45 of chock plug 4 is greater than the height of described elastic component 5, when chock plug 4 being fixed on body 1 end elastic component 5 produce compressive set make the upper surface of elastic component 5 and chock plug 4 against, the lower surface of elastic component 5 and the second electrode 3 against.This electric shock pipe in use, in cavity 1e, the liquid sample of the material comprising cell and cell will be injected is filled in from the opening 1a of body 1, then chock plug 4 is fixed on body 1 end and the annular end face at the inner face of the second electrode 3 and body 1 opening 1a edge is fitted, the inner of first electrode 2 and the second electrode 3 all with liquid communication in cavity 1e, the outer end of the first electrode 2 and the second electrode 3 can electrically connect with outside, first electrode 2 is connected with the pulse power with the second electrode 3, electric discharge makes form electric field in the cavity 1e of electric shock pipe and make cytolemma have certain permeability, thus the target substance in liquid sample is entered in cell.In the technical program, when chock plug 4 is fixed on body 1 end, elastic component 5 between the tubular portion 45 and mount pad 44 of chock plug 4 and also the two ends of elastic component 5 lean with chock plug 4 and the second electrode 3 respectively, elastic component 5 produces compressive set, elastic component 5 is formed with chock plug 4 and the second electrode 3 seal, can avoid occurring gap between the second electrode 3 and the opening 1a of body 1, air is avoided to enter liquid sample in cavity 1e, and when slightly upwarping appears in chock plug 4 time, elastic component 5 also can do certain recovery deformation, ensure that the second electrode 3 still reclines mutually with the end face at opening 1a edge, thus make the liquid sample in cavity 1e still can not produce air bubble when experimenter's application of sample operates some deviation, to sum up, the technical program improves the sealing property between the second electrode 3 and opening 1a effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity 1e.Because elastic component 5 is between the tubular portion 45 and mount pad 44 of chock plug 4, upper and lower two ends lean with chock plug 4 and the second electrode 3 again respectively, therefore there will not be the phenomenon come off.
Body 1 in the present embodiment, chock plug 4 all adopt insulating material to make, and the first electrode 2 and the second electrode 3 adopt electro-conductive material to make, and this part is prior art, and not in this to go forth for the material of embody rule.In addition, the first electrode 2 both can directly be fixed in body 1, also equally with the second electrode 3 can be arranged on inside a chock plug 4 and seal the cavity 1e of body 1.
As shown in Figures 2 and 3, the medial surface of elastic component 5 contacts with the outer side of mount pad 44, the medial surface of elastic component 5 and upper surface contact against forming multidimensional seal with chock plug 4 and are connected, add that the lower surface of elastic component 5 and the second electrode 3 are formed to be tightly connected, thus improve sealing effectiveness further; Convex edge 31a is tapered, and the outer side of convex edge 31a has by spigot surface one 31b of the end face of convex edge 31a face tilt extension laterally; The inner side-wall of mount pad 44 has spigot surface three 45a extended by the inside sidewall slope of the end face of mount pad 44.By the guide effect of spigot surface one 31b and spigot surface three 45a, the installation of the second electrode 3 can be facilitated to place.
As shown in Figures 2 and 3, the periphery of opening 1a has and chock plug 4 can be fixed on body 1 end and make elastic component 5 produce the locating structure of compressive set; This locating structure comprises the connecting cylinder portion 1b be connected as a single entity with the end of body 1, connecting cylinder portion 1b offers the cylinder chamber 1c for chock plug 4 grafting, cylinder chamber 1c chamber wall on there is convex tendon one 1d, the outer side of chock plug 4 has can with the convex tendon 2 43 of convex tendon one 1d phase clamping; By locating structure, elastic component 5 is out of shape and can improves stopping property further, also can effectively prevent chock plug 4 from coming off from body 1 end simultaneously, convex tendon one 1d and convex tendon 2 43 can be complete annular or discontinuous annular is not even annular, can reach the effect of clamping.The protrusion of convex tendon one 1d and convex tendon 2 43 can not be clearly, and the size adopting chock plug 4 to fill in body 1 part is a bit larger tham the size inside connecting cylinder portion 1b, reaches the object of location in an interference fit; Chock plug 4 and the clamping of connecting cylinder portion 1b phase, make both connect be separated all very convenient, improve fluid injection and move the convenience of liquid after completing electric shock, certainly also can adopt the mode be threaded, when adopting screw thread, chock plug 4 rotation can make it be fixed on connecting cylinder portion 1b.
As shown in Figure 3, second electrode 3 comprises the body of rod 31 and cap body 32, one end and the cap body 32 of the body of rod 31 are connected, the other end of the body of rod 31 can be electrically connected by the opening of chock plug 4 and outside and this end also has the convex edge 31a extended along the body of rod 31 radial direction, and the opening part of chock plug 4 has the abutment face 46 leant with convex edge 31a.Convex edge 31a can be passed mount pad 44 by extruding mount pad 44 deformation of external force smoothly and lean with the abutment face 46 at chock plug 4 opening 1a place, after mount pad 44 recovers deformation, convex edge 31a matches with abutment face 46 formation position limiting structure, effectively can prevent coming off of the second electrode 3.Between the inner side-wall of the outer side of elastic component 5 and the tubular portion 45 of chock plug 4, there is gap; Chock plug 4 is fixed on the lower surface of elastic component 5 and the upper surface of cap body 32 during body 1 end to lean, between the periphery of cap body 32 and tubular portion 45 inner side-wall of chock plug 4, also there is gap.Above-mentioned two gaps, place of indwelling can provide certain space for the deformation of elastic component 5, thus utilize the restorer of elastic component 5 to realize the automatic adjustment carrying out shutoff gap between chock plug 4 and the second electrode 3.
Embodiment two:
As shown in Figure 4, the present embodiment is roughly the same with embodiment one, difference is, in the present embodiment, chock plug 4 comprises the tubular portion 45 with through hole 1, the body of rod 31 is plugged in through hole 1, is provided with mount pad 44 in a tubular form in tubular portion 45, and the second above-mentioned electrode 3 is slidably connected on mount pad 44, elastic component 5 is the elastic part of mount pad 44 lower end, the body of rod 31 has the slope 31c leant with elastic component end face with the junction of cap body 32; After the slope 31c of the body of rod 31 is inserted into mount pad 44, slope 31c makes the elastic component 5 on mount pad 44 that elastic deformation occur after inserting mount pad 44, improves stopping property.
Embodiment three:
As shown in Figure 5, the present embodiment is roughly the same with embodiment one, difference is, position limiting structure in the present embodiment comprises the annular protrusion 45b on tubular portion 45 inner side-wall, in the form of annular discs and the external diameter that is cap body 32 of cap body 32 is greater than the internal diameter of annular protrusion 45b, cap body 32 can make the lower surface of cap body 32 be positioned on the upper side of annular protrusion 45b through annular protrusion 45b, can smoothly through the endoporus of annular protrusion 45b after the annular protrusion 45b deformation of cap body 32 in external force extruded tubular part 45, then annular protrusion 45b recovers deformation, the outer face of cap body 32 and the upper side of annular protrusion 45b lean and keep spacing, effectively prevent coming off of the second electrode 3.
Specific embodiment described in literary composition is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various amendment or supplements or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present utility model or surmount the scope that appended claims defines.
Claims (8)
1. an electric shock pipe, described electric shock pipe comprises body (1) and chock plug (4), described body (1) inside has the cavity (1e) for holding target liq sample, one end of described body (1) or middle part are provided with the first electrode (2) be connected with cavity (1e), the other end of described body (1) has the opening (1a) be connected with described cavity (1e), the second electrode (3) is provided with in described chock plug (4), described second electrode (3) comprises the body of rod (31) and cap body (32), the outer face of described cap body (32) can fit with the annular end face at opening (1a) edge, elastic component (5) is provided with between the inner face of described cap body (32) and described chock plug (4), it is characterized in that, the described body of rod (31) to be plugged in described chock plug (4) and to be slidably connected with described chock plug (4), the inner and the described cap body (32) of the described body of rod (31) are connected, the outer end of the described body of rod (31) can be electrically connected by the opening of described chock plug (4) and outside, the position limiting structure that can prevent the body of rod (31) from departing from chock plug (4) is also provided with between described second electrode (3) and chock plug (4), when chock plug (4) is fixed on body (1) end, described elastic component (5) generation compressive set makes the second electrode (3) be pressed on opening (1a).
2. electric shock pipe according to claim 1, it is characterized in that, described position limiting structure comprises the convex edge (31a) that the described body of rod (31) outer end extends along the body of rod (31) radial direction, and described convex edge (31a) radial dimension is more bigger than the bore of described chock plug (4) opening; Described convex edge (31a) can extrude described chock plug (4) under external force and produce deformation and the opening passing described chock plug (4).
3. electric shock pipe according to claim 2, it is characterized in that, the opening part of described chock plug (4) has the abutment face (46) leant with described convex edge (31a).
4. the electric shock pipe according to Claims 2 or 3, it is characterized in that, (31a) is tapered in described convex edge, and the outer side of described convex edge (31a) has by the spigot surface one (31b) of the end face of convex edge (31a) face tilt extension laterally.
5. electric shock pipe according to claim 1, it is characterized in that, described chock plug (4) comprises the tubular portion (45) with through hole one (41), the described body of rod (31) is plugged in described through hole one (41), described position limiting structure comprises the annular protrusion (45b) on tubular portion (45) inner side-wall, in the form of annular discs and the external diameter that is described cap body (32) of described cap body (32) is greater than the internal diameter of described annular protrusion (45b), described cap body (32) can make the outer face of cap body (32) be positioned on the upper side of annular protrusion (45b) through described annular protrusion (45b).
6. the electric shock pipe according to claim 1 or 2 or 3, it is characterized in that, described chock plug (4) comprises the tubular portion (45) with through hole one (41), the described body of rod (31) is plugged in described through hole one (41), mount pad (44) is in a tubular form provided with in described tubular portion (45), above-mentioned the second electrode (3) is slidably connected on mount pad (44), described elastic component (5) is set in described mount pad (44) outward, the height of described elastic component (5) is greater than the height of mount pad (44), between the periphery of described cap body (32) and tubular portion (45) inner side-wall of described chock plug (4), there is gap, when chock plug (4) being fixed on body (1) end described elastic component (5) produce compressive set make the upper surface of elastic component (5) and chock plug (4) against, the lower surface of elastic component (5) and the upper surface of described cap body (32) against.
7. the electric shock pipe according to claim 1 or 2 or 3, it is characterized in that, described chock plug (4) comprises the tubular portion (45) with through hole one (41), the described body of rod (31) is plugged in described through hole one (41), mount pad (44) is in a tubular form provided with in described tubular portion (45), above-mentioned the second electrode (3) is slidably connected on mount pad (44), described elastic component (5) is the elastic part of described mount pad (44) lower end, the described body of rod (31) junction that is upper and cap body (32) has the slope (31c) leant with described elastic component end face.
8. the electric shock pipe according to claim 1 or 2 or 3, it is characterized in that, the periphery of described opening (1a) has can be fixed on body (1) end by chock plug (4) and make described elastic component (5) produce the locating structure of compressive set, described locating structure comprises the connecting cylinder portion (1b) be connected as a single entity with the end of described body (1), described connecting cylinder portion (1b) offers the cylinder chamber (1c) for described chock plug (4) grafting, the chamber wall of described cylinder chamber (1c) has convex tendon one (1d), the outer side of described chock plug (4) has can with the convex tendon two (43) of described convex tendon one (1d) phase clamping.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981250.8U CN205205150U (en) | 2015-12-01 | 2015-12-01 | Electric shock pipe |
| JP2017530204A JP6629858B2 (en) | 2014-12-02 | 2015-12-02 | Electric shock pipe and electroporation apparatus having the electric shock pipe |
| PCT/IB2015/059297 WO2016088062A1 (en) | 2014-12-02 | 2015-12-02 | An electric shock pipe and cell electroporator having electric shock pipe |
| US15/532,182 US10472601B2 (en) | 2014-12-02 | 2015-12-02 | Shock tube and cell electroporation device with the shock tube |
| EP15864958.2A EP3228695A4 (en) | 2014-12-02 | 2015-12-02 | An electric shock pipe and cell electroporator having electric shock pipe |
| US16/586,989 US11965149B2 (en) | 2014-12-02 | 2019-09-29 | Shock tube and cell electroporation device with the shock tube |
| JP2019219959A JP6838125B2 (en) | 2014-12-02 | 2019-12-04 | An electric shock pipe and an electroporation device having the electric shock pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981250.8U CN205205150U (en) | 2015-12-01 | 2015-12-01 | Electric shock pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205205150U true CN205205150U (en) | 2016-05-04 |
Family
ID=55842878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520981250.8U Active CN205205150U (en) | 2014-12-02 | 2015-12-01 | Electric shock pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205205150U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110885857A (en) * | 2018-09-11 | 2020-03-17 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
| CN114196532A (en) * | 2021-11-01 | 2022-03-18 | 上海盟德生物科技有限公司 | Multi-channel cell electrotransformation instrument |
-
2015
- 2015-12-01 CN CN201520981250.8U patent/CN205205150U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110885857A (en) * | 2018-09-11 | 2020-03-17 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
| CN110885857B (en) * | 2018-09-11 | 2024-01-26 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
| CN114196532A (en) * | 2021-11-01 | 2022-03-18 | 上海盟德生物科技有限公司 | Multi-channel cell electrotransformation instrument |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101084528B1 (en) | Pipette tip for electroporation device | |
| US20160227841A1 (en) | Atomizer and electronic cigarette having same | |
| CN205205150U (en) | Electric shock pipe | |
| EP2003435A4 (en) | An apparatus for detecting sealability of a lithium ion cell | |
| CN105411584B (en) | It is a kind of to concentrate for wet brain electricity cap and flexible micro- ooze electrode structure | |
| CN208852154U (en) | Medical device and component including the medical device | |
| HK1112386A1 (en) | Method for making a reservoir containing an active substance diffused through the reservoir and installation therefor | |
| JP6838125B2 (en) | An electric shock pipe and an electroporation device having the electric shock pipe | |
| CN104621718B (en) | Inhalator and its atomizing component | |
| CN104403943B (en) | A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe | |
| CN205205151U (en) | Electric shock pipe | |
| CN204311066U (en) | The cell electroporation of electric shock pipe is managed and had to a kind of electric shock | |
| CN208838883U (en) | Calparine cap | |
| CN102306741A (en) | Injection method for battery electrolyte | |
| CN204516838U (en) | Column type battery fluid injection tool | |
| CN214179126U (en) | Disposable electronic cigarette sealing structure | |
| CN218961482U (en) | NanoFil injector with RPE injection kit | |
| CN208535154U (en) | A kind of novel seal plug taper sealing structure | |
| CN203544633U (en) | Packaging flexible tube for medical conducting adhesive | |
| CN217885094U (en) | Oil dripping jig for oil storage cotton of electronic cigarette | |
| CN215875957U (en) | Needle seat for micro-needle | |
| CN215065637U (en) | A drip piece ware for cell division phase | |
| CN206697547U (en) | A kind of stubborn rich solution electrokinetic cell vent plug frock | |
| CN210813149U (en) | Injection pen for treating phospholipid-resistant syndrome | |
| CN208525654U (en) | Implanted cardiac pacemaker connector sealing structure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |