CN114196532A - Multi-channel cell electrotransformation instrument - Google Patents

Multi-channel cell electrotransformation instrument Download PDF

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Publication number
CN114196532A
CN114196532A CN202111281879.8A CN202111281879A CN114196532A CN 114196532 A CN114196532 A CN 114196532A CN 202111281879 A CN202111281879 A CN 202111281879A CN 114196532 A CN114196532 A CN 114196532A
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module
cell
channel
lower electrode
plate
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朱海龟
王竞
邓小勇
常庆
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Shanghai Mengde Biotechnology Co ltd
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Shanghai Mengde Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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Abstract

The invention discloses a multi-channel cell electrotransfer instrument, which comprises a shell, a touch display module, a power supply module, a microcontroller module, a multi-channel electrotransfer module and a driving module, wherein the multi-channel electrotransfer module and the driving module are arranged in the shell, and the driving module is arranged in XYZ-axis driving and used for driving the multi-channel electrotransfer module to move. The invention discloses a multi-channel cell electrotransformation instrument, which can realize electroporation transfection of 1-6 cell samples under independent conditions. The electrotransfer instrument comprises a unique sample box and electrodes, can carry out in-situ electroporation and exogenous substance introduction on cell samples growing on a plurality of embedding dishes, can not interfere with each other, can simultaneously complete the operation of a plurality of samples, improves the comparability between the samples and greatly improves the experimental efficiency.

Description

Multi-channel cell electrotransformation instrument
Technical Field
The invention relates to the field of biological instrument equipment, in particular to a multi-channel cell electrotransformation instrument.
Background
The electroporation technology is a technology which generates membrane pores communicating the inside and outside of cells by forming a potential difference on two sides of a cell membrane through an external electric field and disturbing the arrangement of phospholipid bilayers. The number, location, size, duration, and recovery of the pores is dependent on a number of factors. Including the strength of the electric field, the energy distribution, the physical and chemical environment of the cell, the size and state of the cell, the local structure of the cell membrane, etc. During the duration of the pore, there is a certain probability that foreign substances, such as plasmids, RNA, proteins, or polysaccharides, will enter the cell. Therefore, the electroporation technology has wide application in the fields of gene editing, embryo transformation, cell mechanism research, protein production, cell therapy and the like.
Electroporation transfection has an irreplaceable advantage over other transfection means. Conventional chemical transfection methods, such as calcium phosphate precipitation, liposome (Lipofectamine 2000, etc.), cationic polymer (PEI, etc.), rely on the phagocytic capacity of cells, and the vector escapes into the cells via lysosomes, making transfection possible. Therefore, the transfection efficiency of the chemical method is low for cells with weak phagocytic ability, such as primary cells, suspension cells, etc., and cells with strong lysosomal ability, such as macrophages, etc. For such recalcitrant cells, a method of viral infection often has to be used. However, the virus selection and preparation period is long, the operation is complicated, and the cost is high. There is also a limit to the size of the inserted fragment, which cannot exceed 2500 bps. However, many common editing tools, such as CRISPR-Cas9, need to be transformed into large-fragment plasmids, which limits the application of virus infection to some extent. In addition, since the principle of viral infection is to change the genome, there is a great risk, and it is difficult to apply the virus to clinical development. Electroporation transfection efficiently transfects recalcitrant cells, has no restriction on plasmid size, is transient transfection, has a low probability of being embedded into the genome, and is often used as a complementary means to chemical and viral methods.
Among the current electrotransformers, there are the Gene Pulser Xcell from Bio-Rad, the Neon system from Thermo Fisher, and the Nucleofector from Lonza, among others. The Bio-Rad electrotransfer instrument adopts a cuvette-type electrotransfer plate, can output simple square waves or exponential decay waves, and reduces the death rate of cells by using an electrotransfer buffer solution. The Neon system changes the electric rotating plate into the electric rotating suction head on the basis, reduces the distance between electrodes, makes the electric field more uniform, and thereby improves the survival rate of cells. The Nucleofector adds a reagent for promoting the plasmid to enter the cell nucleus, namely a nuclear transfer reagent, into the electrotransfer buffer solution so as to improve the transfection efficiency. And different formulas of electrotransfer buffer solutions are provided aiming at different cells so as to improve the survival rate of the cells. Although there is some improvement, these instruments do not fundamentally solve the problem of electrical transfer randomness, do not have qualitative improvements in transfection efficiency and cell survival rate, and have large differences between experimental results, which are difficult to predict. In a typical experiment, a plurality of samples are often required to be tested, and a common electrotransformation machine can only operate one sample at a time, so that the parallel comparison among the samples is not facilitated, and the operation is more complicated.
Disclosure of Invention
In view of the above-mentioned defects of the prior art, the technical problems to be solved by the present invention are the problems of randomness of electroporation, low transfection efficiency, low cell survival rate, inaccurate experimental results, etc. existing in the conventional electroporation apparatus, and the simultaneous electroporation transfection of a plurality of cell samples is realized. The invention provides a multi-channel cell electrotransfer instrument, which is used for performing electroporation and exogenous substance introduction on cells growing on a plurality of embedding dishes. The device can be used for respectively and independently operating 1-6 samples embedded into the vessel at one time, the samples cannot interfere with each other, the comparability among the samples is enhanced, the experimental efficiency is greatly improved, and the operation is simplified.
In order to achieve the above object, the present invention provides a multi-channel cell electric transfer instrument, which comprises a housing, a touch display module, a power module, a microcontroller module, a multi-channel electric transfer module and a driving module, wherein the multi-channel electric transfer module and the driving module are arranged inside the housing, and the driving module is configured to be driven by XYZ axes and is used for driving the multi-channel electric transfer module to move.
Furthermore, the multi-channel electric rotating module comprises an upper electrode contact, an electric rotating plate, a lower electrode contact and a drawing module, wherein the upper electrode contact, the electric rotating plate, the lower electrode contact and the drawing module are sequentially arranged from top to bottom, and the driving module is connected with the upper electrode contact and drives the upper electrode contact to move; the electric rotating plate is arranged on the lower electrode contact, the lower electrode contact is arranged on the drawing module, and the drawing module is used for enabling the electric rotating plate and the lower electrode contact to be connected with the multi-channel cell electric rotating instrument in a drawing mode.
Furthermore, the electrotransfer plate comprises a porous plate, an upper electrode, a lower electrode, a sealing ring and a cell embedding dish, wherein the porous plate comprises a plurality of hole sites, one hole site is a channel, the hole site is used for placing the cell embedding dish, after the cell embedding dish is placed in the hole site, the dish bottom of the cell embedding dish is contacted with the lower electrode, the dish bottom of the cell embedding dish is made of a nano-pore membrane and is provided with a nano microchannel, and cells embedded into the dish are cultured on the nano-pore membrane; the lower electrode of the hole site is used as the hole bottom, the sealing ring is used for sealing the hole bottom of the porous plate, and the upper electrode is used as the hole top of the hole site.
Furthermore, the drawing module is arranged as a drawer, and the lower electrode contact is fixedly connected with the drawer.
Further, the lower electrode contact comprises a groove position plate, a plurality of electrode columns and a pressure spring, the electrode columns are installed on the groove position plate through the pressure spring, and the electrode columns are arranged in parallel.
Further, when the electrotransfer plate is placed on the position plate, the lower electrodes are in contact with the electrode posts and each lower electrode is in contact with one or more electrode posts.
Further, the upper electrode contact comprises an electrode column, and the electrode column is arranged to be in contact with the upper electrode of the electric rotating plate under the driving of the driving module.
Further, the multi-channel electric rotating module comprises a sensor module which is arranged to detect whether the multi-channel electric rotating module is in place.
Furthermore, the material of the nano-pore membrane of the cell embedding vessel is set to be one of plastic, high molecular material, more molecular material and metal, the thickness of the nano-pore membrane is set to be 0-10mm, and the pore diameter of the micro-channel of the nano-pore membrane is 0-1000 μm.
Furthermore, the nano-pore membrane micro-channel pores are set to be manufactured by the processes of particle accelerated impact, etching, weaving, laser drilling and the like.
Technical effects
1. The multichannel microchannel cell electrotransformation instrument is simple to operate, cell culture is completed in a cell embedding dish, the cell embedding dish is placed in a hole position of a porous plate, a positive electrode is placed in the hole position, a drawer is closed, the positive electrode is pressed by moving a positive electrode column downwards through a touch display screen, the positive electrode column is firmly contacted with the positive electrode under the tension action of a pressure spring, and the negative electrode column is firmly contacted with a negative electrode column, so that the cell electrotransfection instrument is electrically connected with a microcontroller unit, and the energy level, the input time and the like of pulse voltage are input, so that the accurate electrotransfection process can be completed in a very short time;
2. the cells are embedded into the dish to complete cell culture, the cells are positioned on the nanopore membrane, and the exogenous substances can be directionally introduced into the cells under the action of an electric field, so that the introduction efficiency of the exogenous substances is improved, and the experiment shows that the introduction rate is improved from 25% to about 90%;
3. the low voltage that little the control unit sent combines stage pulse, and the control of limit and the electric field of corresponding intensity of timely release have improved the survival rate of cell, have improved more than 90% from 25%.
The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, the features and the effects of the present invention.
Drawings
FIG. 1 is a schematic diagram of a multi-channel electrical transfer module and a driving module of a multi-channel cell electrical transfer apparatus according to a preferred embodiment of the present invention;
FIG. 2 is a schematic diagram of an electric rotating plate structure of a multi-channel cell electric rotating machine according to a preferred embodiment of the present invention;
FIG. 3 is a schematic enlarged view of a portion of an electric transfer plate of a multi-channel cell electric transfer apparatus according to a preferred embodiment of the present invention;
FIG. 4 is a schematic diagram of the structure of the lower electrode contact of the multi-channel cell electrotransfer device according to a preferred embodiment of the present invention;
FIG. 5 is a partially enlarged view of the lower electrode contact of the multi-channel cell relay device according to the preferred embodiment of the present invention;
FIG. 6 is a schematic diagram of the housing of a multi-channel cell electrotransfer apparatus according to a preferred embodiment of the present invention.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the following description, for purposes of explanation and not limitation, specific details are set forth, such as particular internal procedures, techniques, etc. in order to provide a thorough understanding of the embodiments of the invention. It will be apparent, however, to one skilled in the art that the present invention may be practiced in other embodiments that depart from these specific details. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present invention with unnecessary detail.
The invention discloses a multi-channel cell electrotransfer instrument, which comprises a shell 26, a touch display module 24, a power supply module, a microcontroller module, a multi-channel electrotransfer module and a driving module, wherein the multi-channel electrotransfer module and the driving module are arranged in the shell, and the driving module is arranged in XYZ-axis driving and used for driving the multi-channel electrotransfer module to move, as shown in figure 6.
As shown in fig. 1, the multi-channel electrical rotating module comprises an upper electrode contact 4, an electrical rotating plate 7, a lower electrode contact 6 and a drawing module 8, wherein the upper electrode contact 4, the electrical rotating plate 7, the lower electrode contact 6 and the drawing module 8 are sequentially arranged from top to bottom, and a driving module is connected with the upper electrode contact and drives the upper electrode contact to move; the electricity changes the board and sets up to install on the lower electrode contact, and the lower electrode contact sets up on the pull module, and the pull module is used for making electricity change board, lower electrode contact and multichannel microchannel cell electrotransformation appearance pull to be connected, and in this embodiment, the pull module sets up to the drawer, lower electrode contact and drawer fixed connection.
As shown in fig. 2 and 3, the electrotransfer plate 7 comprises a porous plate 15, an upper electrode 13, a lower electrode 12, a sealing ring 14 and a cell embedding dish 11, wherein the porous plate comprises a plurality of hole sites, one hole site is a channel, the hole site is used for placing the cell embedding dish 11, the bottom of the cell embedding dish 11 is made of a nano-pore membrane and is provided with a nano-microchannel, and liquid cells in the cell embedding dish are cultured on the nano-pore membrane; the lower electrode 12 at the hole site serves as the bottom of the well, which is sealed with a seal ring 14, and is used for injecting a liquid exogenous substance (plasmid, RNA, protein, polysaccharide, or the like) to be transferred. The upper electrode 13 serves as a hole top of the hole site. The number of the hole sites of the electric rotating plate can be 1-96, and 6 hole sites, namely 6 channels, are selected in the embodiment. The bottom of each hole of the electric rotating plate is provided with a lower electrode which is arranged at the bottom of the porous plate in the following way: the periphery of the lower electrode is sleeved with a soft rubber sealing ring, the lower electrode and the soft rubber sealing ring are placed in a porous plate die cavity and are integrally injection-molded with a porous plate, one cell embedding vessel and an upper electrode can be placed at each hole site above the porous plate, the materials of the lower electrode and the upper electrode are not limited and can be conductors such as graphite, metal, conductive plastics and the like, the materials of the lower electrode and the upper electrode in the embodiment are manufactured by processing low-resistance graphite, and the resistivity of the lower electrode and the upper electrode must be less than 10-5Omega.m, the surface of the lower electrode is provided with a liquid groove, the depth of the liquid groove is 0-1000mm, the diameter of the liquid groove is 0-1000mm, the shape of the notch of the liquid groove can be a cylindrical cavity, a rectangular cavity, a polygonal cavity and an elliptical cavity, the end surface of the upper electrode, which is contacted with the liquid sample, can be a plane, an arc surface, a vertebral body surface and a polygonal surface, and the distance between the upper electrode and the nano-porous membrane is 0-1000 mm.
In this embodiment, the cell embedding dish is made of insulating material, the flange of the dish mouth can be circular, square, oval or polygonal, the bottom of the cell embedding dish is welded with the nano-pore membrane, the welding can be hot-melt welding, hot-press welding, adhesive welding, laser welding, ultrasonic welding or high-frequency welding, the nano-pore membrane can be made of plastic, high-molecular materials, more than molecular materials or metal, the thickness of the nano-pore membrane is 0-10mm, the pore diameter of the nano-pore membrane microchannel is 0-1000 μm, and the nano-pore membrane microchannel can be manufactured by processes such as accelerated impact, etching, weaving and laser drilling through chestnuts.
As shown in fig. 4 and 5, the lower electrode contact includes a groove plate 16, electrode posts 17 and compression springs 18, the electrode posts 17 are mounted on the groove plate 16 by the compression springs 18, each of the electrode posts is provided with a compression spring, the electrode posts 17 are provided in plurality and arranged in parallel relation, and can be electrically connected with the lower electrode individually. And when the electrode column is pressed, the electrode column can reset under the action of the elastic force of the pressure spring. When the electro-rotating plate is placed on the slot plate, the lower electrodes are in contact with the electrode posts, and each lower electrode is in contact with one or more electrode posts. In this embodiment, 24 electrode columns are installed on the cell site plate, and the resistivity of the electrode columns must be less than 10-5Omega.m. The contact surface of the electrode column and the lower electrode is in a knurling shape, the knurling standard is according to relevant parameters of GB/T6403.3-2008, the knurling can be straight knurling or cross-hatched knurling, and the knurling modulus is less than or equal to 0.5. The contact surface of the electrode column and the lower electrode can be other regular dentate contacts, so that the electrode column and the lower electrode can be contacted fully and firmly more effectively, and the voltage and the current are stable and reliable; the electrode columns can be electrically connected with the lower electrode simultaneously by 1-6. In this embodiment, the electric rotating plate is made for insulating material processing, and electric rotating plate and trench board match each other, are convenient for fix a position the electric rotating plate.
As shown in fig. 1, the upper electrode contact 4 includes an electrode column 5, and the electrode column 5 is disposed to contact the upper electrode of the electro-rotating plate under the driving of the driving module. The electrode column resistivity must be less than 10-5Omega · m, the contact surface of the electrode column and the upper electrode is in a knurling shape, the knurling standard is according to related parameters of GB/T6403.3-2008, the knurling can be straight knurling or cross-hatched knurling, and the knurling modulus is less than or equal to 0.5. The contact surface of the electrode column and the upper electrode can be other regular dentate contacts, so that the electrode column and the upper electrode are in full and firm contact more effectively, and the stability and reliability of voltage and current are ensured.
The driving module comprises a Y-axis driving assembly 1, an X-axis driving assembly 2 and a Z-axis driving assembly 3, the electrode column can move up and down under the driving of the Z-axis driving assembly, the Z-axis driving assembly can move left and right under the driving of the X-axis driving assembly, the X-axis driving assembly can move back and forth under the driving of the Y-axis driving assembly, and the electrode column can move in any direction above the electric rotating plate under the driving of the X-axis driving assembly, the Y-axis driving assembly and the Z-axis driving assembly. The electrode column can move down to press any upper electrode on the electric rotating plate, when the electrode column, the electrode column and the microcontroller unit are electrically connected, the microcontroller unit is electrically connected with the power supply module, and the instrument outputs electric pulses to complete the electroporation leading-in of the sample.
Further, the multi-channel cell electric rotating instrument of the embodiment of the invention further comprises a sensor module 9, the sensor module is arranged for detecting whether the multi-channel electric rotating module is in place, and the sensor module 9 is arranged near the drawer opening.
The application method and the principle of the multi-channel cell electrotransformation instrument provided by the embodiment of the invention are as follows:
when the cell inserting plate is used, liquid cells are cultured in the cell inserting dish for a certain time in advance, the cells are attached to the pore-containing membrane, a proper amount of liquid exogenous substances are injected into the hole sites of the electric rotating plate in advance, so that the upper electrode and the lower electrode are ensured to form conductive connection capable of being electrified, the electric rotating plate with good conductive connection is placed on the groove site plate, the lower electrode is contacted with the electrode columns at the moment, each lower electrode is contacted with 4 electrode columns, then the drawer is closed, the sensor detects that the drawer is closed, the touch display module gives an instruction, the electrode columns can move downwards to press the upper electrode, the electrode columns are stably contacted with the upper electrode under the tension action of the pressure spring, the lower electrode is stably contacted with the electrode columns, so that the lower electrode is electrically connected with the microcontroller unit, the microcontroller unit receives the instruction and outputs pulse voltage, and a controllable stable electric field is formed on two sides of the pore-containing membrane, thereby injecting the exogenous material into the cells.
In the cell electric transfer instrument, a casing is provided with a touch display screen, a user can set electroporation parameters of each sample on the upper section of the touch screen, the touch display screen is connected with a micro control unit, an upper electrode post and a lower electrode post are connected with the micro control unit, the micro control unit is provided with a voltage generation unit, a current detection unit, a pulse switch unit, a programmable singlechip and other microprocessors, and the voltage generation unit and the pulse switch unit emit pulses with extremely low voltage to complete electroporation lead-in.
The use process of the multi-channel cell electrotransformation instrument is as follows:
1. and (4) ejecting the drawer, embedding 1-6 cells into the hole positions of the porous plate placed in the dish, placing the upper electrode, withdrawing the drawer, and contacting the lower electrode with a lower electrode contact inside the instrument. And setting parameters through the touch display screen. After the click starts, the upper electrode contact moves down to contact the upper electrode of the first sample, and a passage is formed. The instrument outputs electric pulses to complete the electroporation introduction of the first sample;
2. after the first sample is finished, the micro control unit moves by combining with the transmission device and repositions the upper electrode contact to the next sample, and finishes the operation of the second sample according to the set parameters, and so on;
3. within a few seconds, after all samples are finished, the drawer is popped up, and the cell samples can be taken out and put back to be cultured continuously.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims (10)

1. The utility model provides a cell electrotransformation appearance of multichannel, includes shell, touch-control display module, power module and microcontroller module, its characterized in that still includes multichannel electrotransformation module and drive module, multichannel electrotransformation module with drive module sets up inside the shell, wherein, drive module sets up to XYZ axle drive, is used for driving multichannel electrotransformation module removes.
2. The multi-channel cell electrotransfer instrument according to claim 1, wherein the multi-channel electrotransfer module comprises an upper electrode contact, an electrotransfer plate, a lower electrode contact and a pull module, the upper electrode contact, the electrotransfer plate, the lower electrode contact and the pull module are arranged in sequence from top to bottom, and the driving module is connected with the upper electrode contact and drives the upper electrode contact to move; the electric rotating plate is arranged to be installed on the lower electrode contact, the lower electrode contact is arranged on the drawing module, and the drawing module is used for enabling the electric rotating plate, the lower electrode contact and the multi-channel cell electric rotating instrument to be connected in a drawing mode.
3. The multi-channel cell electrotransfer instrument according to claim 2, wherein the electrotransfer plate comprises the multi-hole plate, an upper electrode, a lower electrode, a sealing ring and a cell embedding dish, the multi-hole plate comprises a plurality of hole sites, one hole site is a channel, the hole site is used for placing the cell embedding dish, after the cell embedding dish is placed in the hole site, the dish bottom of the cell embedding dish is in contact with the lower electrode, the dish bottom of the cell embedding dish is made of a nano-hole membrane and is provided with a nano micro channel, and cells in the cell embedding dish are cultured on the nano-hole membrane; the hole site uses the lower electrode as the hole bottom, uses the sealing washer to seal the hole bottom of the porous plate, and the upper electrode is used as the hole top of the hole site.
4. A multi-channel cell relay according to claim 2, wherein the drawing module is configured as a drawer, and the lower electrode contact is fixedly connected to the drawer.
5. A multi-channel cell relay according to claim 3, wherein the lower electrode contact comprises a slot plate, a plurality of electrode columns and a compression spring, the electrode columns are mounted on the slot plate by the compression spring, and the electrode columns are arranged in parallel.
6. A multi-channel cell relay according to claim 5, wherein when said relay plate is placed on said well plate, said lower electrodes are in contact with said electrode posts, and each lower electrode is in contact with one or more of said electrode posts.
7. A multi-channel cell relay according to claim 3, wherein the upper electrode contact comprises an electrode post, and the electrode post is configured to contact the upper electrode of the relay plate under the driving of the driving module.
8. A multi-channel cell relay according to claim 1, further comprising a sensor module configured to detect whether the multi-channel relay is in place.
9. A multi-channel cell electro-conversion device as claimed in claim 3, wherein the nano-porous membrane material of the cell embedding dish is one of plastic, polymer material and metal, the thickness of the nano-porous membrane is set to 0-10mm, and the pore diameter of the micro-channel of the nano-porous membrane is 0-1000 μm.
10. A multi-channel cell electroporation apparatus as claimed in claim 9, wherein the nanopore membrane microchannel aperture is configured to be fabricated by particle accelerated impact, etching, weaving, laser drilling, and the like.
CN202111281879.8A 2021-11-01 2021-11-01 Multi-channel cell electrotransformation instrument Pending CN114196532A (en)

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