CN104403943B - A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe - Google Patents

A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe Download PDF

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CN104403943B
CN104403943B CN201410722470.9A CN201410722470A CN104403943B CN 104403943 B CN104403943 B CN 104403943B CN 201410722470 A CN201410722470 A CN 201410722470A CN 104403943 B CN104403943 B CN 104403943B
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electrode
electric shock
elastic component
chock plug
shock pipe
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CN104403943A (en
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陈剑
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Taizhou Sairui TREK Biological Technology Co.,Ltd.
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Priority to CN201410722470.9A priority Critical patent/CN104403943B/en
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Priority to JP2017530204A priority patent/JP6629858B2/en
Priority to PCT/IB2015/059297 priority patent/WO2016088062A1/en
Priority to US15/532,182 priority patent/US10472601B2/en
Priority to EP15864958.2A priority patent/EP3228695A4/en
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Publication of CN104403943B publication Critical patent/CN104403943B/en
Priority to US16/586,989 priority patent/US11965149B2/en
Priority to JP2019219959A priority patent/JP6838125B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube

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Abstract

The invention provides a kind of cell electroporation shocking by electricity pipe and there is electric shock pipe, belong to biological medicine instrument and equipment technical field. It solve existing cell electricity and turn the technical problems such as poor stability. This electric shock pipe includes body, the first electrode, the second electrode and chock plug, tubular body has the cavity for holding target liq sample, one end of body is provided with the first electrode, second electrode is arranged in chock plug and the outer end of the second electrode can be coupled with external electrical by the opening of chock plug, the elastic component being connected with the second electrode it is provided with in chock plug, the outside of elastic component is connected with chock plug, and the inner side of elastic component is connected with the second electrode. The present invention also provides for a kind of cell electroporation that can place this electric shock pipe. The pipe that shocks by electricity in the present invention can be effectively improved the sealing property between the second electrode and opening, thus avoiding the air extraneous when adding fluid sample to enter in cavity; This cell electroporation is easy to operation, convenient observation.

Description

A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe
Technical field
The invention belongs to biological medicine instrument and equipment technical field, relate to a kind of cell electroporation shocking by electricity pipe and there is electric shock pipe.
Background technology
Cell electroporation (turn also known as cell electrotransfection or cell electricity) be utilize electric pulse to introduce in cell cannot the technology of macromole of permeates cell membranes. Electroporation is a kind of widely used and obtains the method for test cell line and gene therapy of strong preference. When applying highfield, cell membrane is temporarily changed to porous character and can pass through foreign material, such as macromole. Cell membrane electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulse voltage, pulse duration, number of pulses and other experiment conditions.
The equipment being currently used for cell electroporation mainly has cell electroporation, pole cup etc. China patent CN1965079B discloses a kind of electroporation device with long hollow, this electroporation device includes long hollow, to provide uniform electric field in electroporation process, wherein particularly, performing electroporation is by after being full of, in hollow, the liquid sample of material including cell and will inject cell, pair of electrodes applying electric pulses from long two ends of hollow.
Applicant once declared one section of " method and apparatus of electroporation " METHODSANDDEVICESFORELECTROPORATION by name; The United States Patent (USP) of US2013/0052711A1, this patent describes a kind of electric shock pipe, its effect is to add the liquid sample containing cell and the material that will inject cell in pipe, upper/lower terminal at electric shock pipe is respectively provided with electrode and bottom electrode, it is connected with upper electrode and bottom electrode by cell electroporation, so that forming electric field in electric shock pipe, and then extracellular material is made to inject in cell. And being filled in by liquid sample after in electric shock pipe, before starting electric shock, need in electric shock pipe to have prevented air residual from forming aeration CURRENT DISTRIBUTION. In that patent, applicant's design on the tube wall of electric shock pipe has an annular groove being connected with electric shock tube chamber, experimenter sustainable to electric shock tube chamber in inject liquid sample until liquid surface protrude electric shock tube chamber after, to contact until being pressed onto the upper edge of electric shock tube chamber again under upper electrode cap with liquid convex surface, formed the sealing of liquid inside cavity, and the liquid stream overflowed on a small quantity is in annular groove. This design is generally possible to prevent in electric shock pipe residual air impact experiment.
But, applicant finds in constantly practice, and the precise requirements to manufacturing of the electric shock pipe in above-mentioned patent is higher, and the requirement of experimenter's operation simultaneously is also higher. If it is accurate not on manufacturing, it is easy to cause electrode and the poor sealing on edge on cavity, it is possible to after under electrode cap, generating electrodes upwarps and again leaves on cavity along causing that extraneous air reenters the situation of cavity. If being additionally also easily caused in pipe when operator's improper use and having residual air bubble, affect the effect of cell electroporation. Scientific experiment is very high to the reliability requirement of instrument and equipment, and therefore, above-mentioned electric shock pipe needs to increase some designs improving reliability on manufacturing, thus improving the reliability of experimenter's operation.
Summary of the invention
The present invention be directed to the problems referred to above that existing technology exists, it is provided that a kind of electric shock pipe. The technical problem to be solved is: how by improving the sealing property between the second electrode and open end, thus avoiding the air in the external world to enter in cavity.
The present invention be directed to the problems referred to above that existing technology exists, additionally provide a kind of cell electroporation with electric shock pipe, the technical problem to be solved is: how to improve the electroporation performance of this cell electroporation.
First purpose of the present invention can be realized by following technical proposal:
A kind of electric shock pipe, described electric shock pipe includes body, the first electrode, the second electrode and chock plug, described tubular body has the cavity for holding target liq sample, it is characterized in that, one end of described body is provided with described first electrode, the other end of described body has the opening being connected with described cavity, and the working position of described first electrode is connected with described cavity, and the edge of described opening has the end face of annular; Described second electrode is arranged in chock plug and the outer end of described second electrode can be coupled with external electrical by the opening of described chock plug, the inner face of described second electrode can fit with the annular end face of edge of opening, the elastic component being connected with the second electrode it is provided with in described chock plug, the outside of described elastic component is connected with described chock plug, the inner side of described elastic component is connected with described second electrode, and the periphery of described opening has can be fixed on chock plug body end and make described elastic component produce the location structure of compressive deformation.
Its operation principle is as follows: this electric shock is managed in use, the liquid sample that can fill in the material comprising cell and will injecting cell to cavity extremely forms a liquid convex surface, then passing through fixed structure makes chock plug be fixed on body end and make the elastic component between chock plug and the second electrode produce compressive deformation, now the second electrode is crushed on open end, first electrode and the second electrode all with liquid communication in cavity, the first electrode and the second electrode is made to be connected with the pulse power again, electric discharge makes to be formed in electric shock pipe electric field, cell membrane is made to have certain permeability, so that the target substance in liquid sample can enter in cell. when chock plug is fixed on body end, elastic component produces compressive deformation, can avoid, between the second electrode and opening, gap occurs, improve the sealing property between the second electrode and open end, thus avoiding air to enter the liquid sample in cavity, and, when slightly upwarping occurs in chock plug time, elastic component also can do certain recovery deformation, the liquid sample in cavity ensures that the second electrode still reclines mutually with the end face of edge of opening, so that still will not produce air bubble when experimenter's application of sample operates some deviation, in sum, this electric shock pipe can be effectively improved the sealing property between the second electrode and opening, thus avoiding the air extraneous when adding fluid sample to enter in cavity.
Location structure in the application directly provides the stable support in outer end and location for elastic component, elastic component is made to be in stable compressive state between chock plug and the second electrode, second electrode can be applied stable elastic force by such elastic component, make the laminating between the second electrode and the open end of body more closely and stablize, thus the sealing effectiveness enhanced between the second electrode and the open end of body, location structure and elastic component are inseparable, jointly solve the technical problem of " the sealing stability how strengthening between the second electrode and body ".
It should be noted that the body in the application, chock plug adopt insulant to make, the first electrode and the second electrode adopt conductive material to make, and this part is prior art, and not in this to go forth for the material of concrete application. It addition, the first electrode both can be directly anchored in body, it is also possible to the same with the second electrode be arranged on inside a chock plug and again body cavity sealed.
In above-mentioned one electric shock pipe, described chock plug includes the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode includes the body of rod and cap body, one end of the described body of rod is connected with described cap body, the described body of rod other end can pass through the opening of described chock plug and couple with external electrical, described elastic component is socketed on body of rod lateral surface, the outer end of described chock plug has along the radially inner gear edge of through hole one, the outer face of described elastic component is resisted against gear along upper, and the inner face of this elastic component is resisted against in cap body. Described elastic component adopts rubber-plastics material to make, and rubber-plastics material includes plastics, rubber, silica gel etc. When chock plug is fixed on body end, the end face at the second electrode and the cavity hatch edge of body reclines mutually, and elastic component produces compression, improves the sealing of the second electrode and edge of opening end face. The body of rod of described second electrode can pass through opening and the external electrical connections on chock plug gear edge, and the body of rod of the second electrode can extend to the outside of chock plug to the second electrifying electrodes; The described body of rod can also be not extend to the outside of chock plug, and the electric connection contacts outside chock plug can also be put in chock plug by the opening of chock plug and electrically connect with the second electrode. Elastic component generally can make round, it is also possible to is incomplete round even other shapes, if flexible and can grafting the second electrode. In above-mentioned one electric shock pipe, described elastic component can be an independent elastic component, is arranged in an interference fit in through hole one, or is arranged in through hole one in the mode such as bonding and is connected with described chock plug. Described elastic component can interference fit or bonding mode be socketed on the body of rod of described second electrode.
In above-mentioned one electric shock pipe, shoulder block portion one it is provided with between described elastic component and described cap body, described shoulder block portion one between described elastic component and described cap body, the size in described shoulder block portion one or the spacial yardstick diameter more than the body of rod and the diameter less than cap body. One, shoulder block portion cap body and elastic component separate and make cap body compressing elastic component carry out pressure conduction only by shoulder block portion one, and the diameter in shoulder block portion one or be smaller in size than cap body and elastic component, the bigger pressure thus produced on relatively small area of contact promotes elastic component to be easier to deform upon displacement. When chock plug is fixed in location structure, second electrode reclines mutually with open end, elastic component is subject to being in compressive state from the pressure of the second electrode, produce pressure conduction by this compressive deformation and increase sealing property to the open end of body end, and produce when chock plug has and upwarps on a small quantity certain to restore deformation and keep the second electrode to continue to be pressed on the open end of body end.
In above-mentioned one electric shock pipe, described shoulder block portion one can be independent parts, such as one the independent round shoulder block being smaller in size than elastic component and cap body, shoulder block portion one is enclosed within the body of rod between elastic component and cap body, its material can be insulation or nonisulated can.
In above-mentioned one electric shock pipe, as the second situation, described shoulder block portion one is installed in described cap body or described shoulder block portion one and described cap body, the body of rod are one-body molded, and the inner face of described elastic component is resisted against on the end face in described shoulder block portion one. When in body of rod grafting with elastic component time, cap body and elastic component are separated and by its conducting pressure by this shoulder block portion one.
In above-mentioned one electric shock pipe, as the third situation, described shoulder block portion one is installed on described elastic component or described shoulder block portion one is one-body molded with elastic component, and described shoulder block portion one leans with described cap body.
In above-mentioned one electric shock pipe, as in another kind of elastic component scheme, the connected mode of elastic component and described chock plug is for being directly connected to, described chock plug includes the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode includes the body of rod and cap body, one end of the described body of rod is connected with described cap body, the described body of rod other end can pass through the opening of described chock plug and couple with external electrical, described elastic component is have elastic gear along elastic component along what through hole one extended internally out on described through hole one inwall, described gear is resisted against on the lateral surface of the described body of rod along elastic component. this gear is identical with chock plug along the material of elastic component, and the inwall of outside and chock plug main body is connected as a single entity and one opening of intermediate formation, and the body of rod of the second electrode is plugged on keeps off the opening part along elastic component with interference fit or the mode such as bonding. the main body of electric shock pipe is for the needs of degree of accuracy, it is generally required to be made to certain intensity to prevent deformation, and the gear being connected as a single entity with chock plug can adopt the mode reducing this partial intensities to realize elasticity along elastic component as elastic component, this partial design such as become thickness compare other parts thinner it is thus possible to be easier to pressure deformation. this gear generally can make a round along elastic component, it is also possible to is not round, if the elasticity of maintaining and can grafting the second electrode just passable.
In above-mentioned one electric shock pipe, as the first situation, described gear along being provided with shoulder block portion two between elastic component and described cap body, the size in described shoulder block portion two or the spacial yardstick diameter more than the body of rod and the diameter less than cap body. This shoulder block portion two can be independent back-up ring.
In above-mentioned one electric shock pipe, the second situation is that shoulder block portion two is installed in described cap body or described shoulder block portion two is all one-body molded with described cap body, the body of rod, and described gear is resisted against on the end face in described shoulder block portion two along the inner face of elastic component.
In above-mentioned one electric shock pipe, the third situation be shoulder block portion two be installed in described gear along elastic component or described shoulder block portion two one-body molded along elastic component with gear, described shoulder block portion two leans with described cap body.
In above-mentioned one electric shock pipe, scheme as the third kind of elastic component, described elastic component is a stage clip, described chock plug includes the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode includes the body of rod and cap body, one end of the described body of rod is connected with described cap body, the described body of rod other end can pass through the opening of described chock plug and couple with external electrical, described compression spring sleeve is connected on body of rod lateral surface, the outer end of described chock plug has along the radially inner gear edge of through hole one, the outer face of described stage clip is resisted against gear along upper, the inner face of this stage clip is resisted against in cap body. when chock plug is fixed on body end, stage clip can produce compression, improves the sealing property between the body end of the second electrode and edge of opening.
In above-mentioned one electric shock pipe, described location structure includes the connecting cylinder portion being connected as a single entity with the end of described body, described connecting cylinder portion offers the barrel chamber for described chock plug grafting, having convex tendon one in the cavity wall of described barrel chamber, the lateral surface of described chock plug has can with the convex tendon two of described convex tendon one phase clamping. Described convex tendon one and convex tendon two can be complete annular or discontinuous annular is not even annular, can reach the effect of clamping. The protrusion of convex tendon one and convex tendon two can not be it is obvious that adopts what chock plug filled in body portion to be sized slightly larger than the size inside connecting cylinder portion, reaches the purpose positioned in an interference fit; Chock plug and the phase clamping of connecting cylinder portion so that both connect and separate extremely convenients, move the convenience of liquid, certainly may be used without threaded mode after improving fluid injection and completing electric shock, when adopting screw thread, chock plug rotates and it can be made to be fixed on connecting cylinder portion.
In above-mentioned one electric shock pipe, described location structure includes the grab one being arranged on described chock plug, and described body has can with the buckle one of described grab one phase clamping.
In above-mentioned one electric shock pipe, the end face of described edge of opening offers annular groove. This electric shock is managed in use, liquid sample can be filled in cavity, until liquid sample forms a convex surface on opening, again through the second electrode contact liquid convex surface lower means of press seals opening, ensure in cavity without residual air, and form the surplus liquid required for convex surface and flow into annular groove, do not affect cell electroporation process in cavity fluid sample.
In above-mentioned one electric shock pipe, described chock plug is connected by a flexible connecting member with described body. The two ends of flexible connecting member are connected with chock plug and body respectively, chock plug was both connected with body, can relatively swing by body again, it is to avoid because imprudence loses chock plug, the plastic thickness of described flexible piece is made to smaller, it is possible to achieve the flexibility of its wide-angle bending.
In above-mentioned one electric shock pipe, the outside of described elastic component and described chock plug fit to be formed and seal, and the inner side of described elastic component and the lateral surface of the second electrode fit to be formed and seal.
In above-mentioned one electric shock pipe, being provided with ion conductive layer in the cavity of described body, the subsurface of described ion conductive layer and the first electrode contact, the face layer of described ion conductive layer can with target liq sample contact. As above design, the direct contact of cell sample and the first electrode can be separated, thus avoiding cell sample to be subject to the direct infringement of electrochemical reaction near the first electrode, ion conductive layer contains the soluble-salt composition source as ion, gelatinous mass such as agarose, agar, polyacrylamide, colloid albumen etc. can be contained and form gel or semi-solid state, or can infiltrate that saline solution formed containing porous solid can the state of ionic conduction.
Second purpose of the present invention can be realized by following technical proposal:
A kind of cell electroporation with electric shock pipe, described cell electroporation includes casing, is provided with fixing seat, described fixing seat offers jack in described casing; It is characterized in that, described cell electroporation also includes the electric shock pipe being plugged in this jack, described electric shock pipe includes body, the first electrode, the second electrode and chock plug, described tubular body has the cavity for holding target liq sample, one end of described body is provided with described first electrode, the other end of described body has the opening being connected with described cavity, and the working position of described first electrode is connected with described cavity, and the edge of described opening has the end face of annular; Described second electrode is arranged in chock plug and the outer end of described second electrode can be coupled with external electrical by the opening of described chock plug, the inner face of described second electrode can fit with the annular end face of edge of opening, the elastic component being connected with the second electrode it is provided with in described chock plug, the lateral surface of described elastic component is connected with described chock plug, the inner side of described elastic component is connected with described second electrode, and the periphery of described opening has can be fixed on chock plug body end and make described elastic component produce the location structure of compressive deformation; The inner of described jack has the electrode terminal one that can electrically connect with described first electrode, described casing is provided with the cover plate for hiding jack outer end, described cover plate is provided with the electrode terminal two electrically connected with described second electrode, described casing is additionally provided with the power module electrically connected with described electrode terminal one and electrode terminal two.
Its operation principle is as follows: this cell electroporation is in use, can liquid sample be filled in the cavity of body, then chock plug is covered, shock by electricity the cover plate that then closes after pipe is placed in jack, making electrode terminal one electrically connect with the first electrode, electrode terminal two electrically connects with the second electrode, then starts power pulse electric discharge, make to be formed in electric shock pipe electric field, and then make extracellular material inject in cell. Owing to being additionally provided with the elastic component for strengthening the active force between the second electrode and open end in chock plug, therefore can avoid, between the second electrode and opening, gap occurs; Further, when the phenomenon that chock plug appearance slightly upwarps, elastic component also can do certain recovery deformation, it is ensured that the second electrode still reclines mutually with the body end of open end, thus avoiding extraneous air to enter cavity; Also make the electric shock pipe bubble that oneself electrochemical reaction internal produces among electric shock process suffer restraints simultaneously. In sum, electric shock pipe in this cell electroporation can be effectively improved the sealing property between the second electrode and open end, turn thus avoiding air extraneous in electric shock set-up procedure to enter formation aeration electricity in electric shock tube chamber, and reduce the bubble of the electrochemical reaction generation impact on electric current in electric shock process, thus improving the electroporation performance of this cell electroporation.
In above-mentioned a kind of cell electroporation with electric shock pipe, described fixing seat includes pedestal and clamping cylinder, offers described jack in described clamping cylinder, offers a slot in described pedestal, and described clamping cylinder is inserted in slot and both detachably interconnectings. Clamping cylinder and slot removably connect, then the diameter of the pipe that can shock by electricity according to difference, change the clamping cylinder of corresponding jack pore size, can improve the versatility of this cell electroporation.
In above-mentioned a kind of cell electroporation with electric shock pipe, described clamping cylinder is made up of the light transmittance material more than 50%.
In above-mentioned a kind of cell electroporation with electric shock pipe, the bottom land of the bottom of described clamping cylinder and described slot reclines mutually, and the top of described clamping cylinder is provided with at least one lever. Design facilitates operator's mounting or dismounting clamping cylinder after having lever. In order to adapt to shorter electric shock pipe, metal electrode extension device can also be installed below clamping cylinder, contact with electrode terminal one below this metal extension device, after shorter electric shock pipe inserts clamping cylinder, the first electrode realizes electrically connecting by metal extension device with electrode terminal one, so supplements the deficiency of electric shock pipe height.
In above-mentioned a kind of cell electroporation with electric shock pipe, an inner chamber it is further opened with in described pedestal, the bottom land of described slot offers through hole two, the two ends of described through hole two respectively with described jack and intracavity inter-connection, described inner chamber is provided with spring base and spring, the inner of described spring is fixed on spring base, and the outer end of described spring is connected with the inner of described electrode terminal one, and the outer end of described electrode terminal one can be passed described through hole two and be inserted in described jack. After design has spring base and spring, when cover plate covers jack openings, first electrode and the second electrode can electrically connect with electrode terminal one and electrode terminal two respectively, cover plate push down electric shock pipe time described spring compressed generation bigger extra pressure and this pressure is transmitted to described cavity hatch via the second electrode, strengthen the sealing of the second electrode and cavity hatch. Due to electrochemical reaction electrolysis meeting creating pockets of air in cell electric shock process, when between the second electrode and cavity hatch, pressure is relatively larger, these electrochemistry bubbles can be compressed and be reduced it to the impact of CURRENT DISTRIBUTION in electric shock process, thus improving cell electroporation effect. Cell has shocked by electricity back shroud when opening, and electric shock pipe can eject a part, convenient pickup under the action of the spring from jack.
In above-mentioned a kind of cell electroporation with electric shock pipe, described cover plate is made up of the light transmittance material more than 50%, described pedestal adnexa is provided with the sensor for detecting electric shock pipe change in displacement, the micro-control unit electrically connected with sensor it is provided with in described casing, it is additionally provided with display lamp in described casing, described display lamp is also connected with described power module, and the signal of described sensor can pass to described micro-control unit for controlling display lamp. Display lamp is except having instruction electric shock pipe connection status effect, can also there is illuminating effect simultaneously, the display lamp such as LED etc. that can adopt higher brightness illuminates electric shock pipe, and sensor can trigger switch for mechanicalness, and the depression bar linked with it when electrode terminal one is subjected to displacement triggers switch; Sensor can also be photoswitch, when electrode terminal one is subjected to displacement, light path is produced impact and triggers photoswitch; Sensor can also Hall switch etc., trigger when electric shock pipe or electrode terminal one are subjected to displacement.
In above-mentioned a kind of cell electroporation with electric shock pipe, described display lamp may be mounted in the casing near electric shock pipe jack, and light illuminates electric shock pipe nearby. Described display lamp can also be arranged on fixing seat, described fixing seat can be opened horizontal through hole to install display lamp, described clamping cylinder can be made by transparent material, and the display lamp on fixing seat can pass through clamping cylinder and illuminate electric shock pipe from the side, is formed and compares visual effect clearly. Further, various sizes of transparent clamping cylinder is adapted to various sizes of electric shock pipe.
In above-mentioned a kind of cell electroporation with electric shock pipe, described casing is provided with a display screen, it is provided with sampler in described casing, described micro-control unit electrically connects with display screen and power module, described sampler respectively with electric shock pipe and micro-control unit electrically connect, described samplers sample to the signal of telecommunication can pass to micro-control unit and show with the form of wavy curve on a display screen. Power module can produce the electric pulse that cell electroporation needs, casing is provided with display screen and for display instrument and experiment information and experimental implementation interface can be shown, micro-control unit in casing can control power module and display screen, micro-control unit includes programmable single-chip microcomputer and other microprocessors etc., sampler can gather the signal of telecommunication in cell electroporation process, including voltage or current signal, sampler includes the electronic components such as resistance, the signal of telecommunication processes via micro-control unit, it is possible to show on a display screen with the form of oscillogram.
In above-mentioned a kind of cell electroporation with electric shock pipe, the inner of described cover plate is articulated with in casing, and the outer end of described cover plate is provided with buckle two, and described casing is additionally provided with can with the grab two of described buckle two-phase clamping. Cover plate one end is articulated with in casing, the other end and casing phase clamping, can conveniently cover and open.
Compared with prior art, the invention have the advantages that
1, when chock plug is fixed on body end, elastic component produces compressive deformation, can avoid, between the second electrode and opening, gap occurs, improve the sealing property between the second electrode and open end, thus avoiding air to enter the liquid sample in cavity, and, when the phenomenon that chock plug appearance slightly upwarps, elastic component also can do certain recovery deformation, the liquid sample in cavity ensures that the second electrode still reclines mutually with the end face of edge of opening, so that still will not produce air bubble when experimenter's application of sample operates some deviation; In sum, this electric shock pipe can be effectively improved the sealing property between the second electrode and opening, thus avoiding the air extraneous when adding fluid sample to enter in cavity;
2, in the application, cell electroporation cover plate produces extra pressure when pushing down electric shock pipe and is transmitted to the body end of edge of opening via the second electrode, strengthen the sealing of the second electrode and edge of opening, the electrochemistry bubble that in cell electric shock process, solution electrochemistry reaction produces can be compressed to a certain extent, thus reducing it to the impact of CURRENT DISTRIBUTION in electric shock process, thus improving cell electroporation effect;
3, in the application, cell electroporation can allow experimenter to observe the state of electric shock pipe, contributes to experimenter and observes the carrying out with Control release;
4, in the application cell electroporation can actual current in showed cell electroporation process or voltage waveform, contribute to experimenter and observe the carrying out with Control release.
Accompanying drawing explanation
Stereochemical structure when Fig. 1 is that in embodiment one, the second electrode do not installed by this electric shock pipe is shown
It is intended to.
Cuing open of electric shock pipe when Fig. 2 is that in embodiment one, chock plug is not fixed in location structure
View.
Fig. 3 is the partial enlarged drawing in embodiment one.
Fig. 4 is the partial enlarged drawing in embodiment two.
Fig. 5 is the partial enlarged drawing in embodiment three.
Fig. 6 is the sectional view of second electrode electric shock pipe when being fixed in location structure with chock plug.
Fig. 7 is partial enlarged drawing in embodiment four.
Fig. 8 is partial enlarged drawing in embodiment five.
Fig. 9 is partial enlarged drawing in embodiment six.
Figure 10 is partial enlarged drawing in embodiment seven.
Figure 11 is the structural representation of this cell electroporation in embodiment eight.
Figure 12 is the partial enlarged drawing of Figure 11.
Figure 13 is the explosive view of this cell electroporation in embodiment eight.
Figure 14 is the fundamental diagram of this cell electroporation in embodiment eight.
Figure 15 is the axonometric drawing of this cell electroporation in embodiment eight.
In figure, 1, body; 1a, opening; 1b, connecting cylinder portion; 1c, barrel chamber; 1d, convex tendon one; 1e, cavity; 1f, annular groove; 1g, ion conductive layer; 2, the first electrode; 3, the second electrode; 31, the body of rod; 32, cap body; 33, shoulder block portion one; 34, shoulder block portion two; 4, chock plug; 41, through hole one; 42, gear edge; 43, convex tendon two; 5, elastic component; 6, stage clip; 7, casing; 8, fixing seat; 81, pedestal; 811, slot; 82, clamping cylinder; 821, jack; 822, lever; 9, cover plate; 10, electrode terminal one; 11, electrode terminal two; 12, inner chamber; 13, through hole two; 14, spring base; 15, spring; 16, buckle two; 17, grab two; 18, keep off along elastic component; 21, electric shock pipe; 24, sampler; 25, micro-control unit; 26, display screen; 27, power module; 28, display lamp; 29, sensor; 30, flexible connecting member.
Detailed description of the invention
The following is specific embodiments of the invention and in conjunction with accompanying drawing, technical scheme is further described, but the present invention is not limited to these embodiments.
Embodiment one:
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 6, this electric shock pipe includes body the 1, first electrode the 2, second electrode 3 and chock plug 4, chock plug 4 is connected by a flexible connecting member 30 with body 1, body 1 is internal has the cavity 1e for holding target liq sample, one end of body 1 is provided with the first electrode 2, the other end of body 1 has the opening 1a being connected with cavity 1e, and the working position of the first electrode 2 is connected with cavity 1e, and the edge of opening 1a has the end face of annular, second electrode 3 is arranged in chock plug 4 and the outer end of the second electrode 3 can be coupled with external electrical by the opening of chock plug 4, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, it is provided with elastic component between chock plug 4 and the second electrode 3, the lateral surface of elastic component leans with chock plug 4 or is connected, medial surface and second electrode 3 of elastic component lean, the periphery of opening 1a has can be fixed on chock plug 4 body 1 end and make elastic component produce the location structure of compressive deformation, location structure includes the connecting cylinder portion 1b being connected as a single entity with the end of body 1, connecting cylinder portion 1b offers the barrel chamber 1c for chock plug 4 grafting, the cavity wall of barrel chamber 1c has convex tendon one 1d, having on the lateral surface of chock plug 4 can with the convex tendon 2 43 of convex tendon one 1d phase clamping, in the present embodiment, convex tendon one 1d and convex tendon 2 43 are complete annulars, can also is that other shapes such as some sections of arc compositions.
When using this electric shock pipe, the liquid sample filling in the material comprising cell and will injecting cell to cavity 1e extremely forms a liquid convex surface, then passing through fixed structure makes chock plug 4 be fixed on body 1 end and make the elastic component between chock plug 4 and the second electrode 3 produce compressive deformation, now the second electrode 3 is crushed on opening 1a end face, first electrode 2 and the second electrode 3 all with liquid communication in cavity 1e, the first electrode 2 and the second electrode 3 is made to be connected with the pulse power again, electric discharge makes to be formed in electric shock pipe 21 electric field, cell membrane is made to have certain permeability, so that the target substance in liquid sample can enter in cell. when chock plug 4 is fixed on body 1 end, elastic component produces compressive deformation, can avoid, between the second electrode 3 and opening 1a, gap occurs, improve the sealing property between the second electrode 3 and opening 1a end face, thus avoiding air to enter the liquid sample in cavity 1e, and, when the phenomenon that slightly upwarps occurs in chock plug 4, elastic component also can do certain recovery deformation, the liquid sample in cavity 1e ensures that the second electrode 3 still reclines mutually with the end face at opening 1a edge, so that still will not produce air bubble when experimenter's application of sample operates some deviation, it is effectively improved the sealing property between the second electrode 3 and opening 1a, thus avoiding the air extraneous when adding fluid sample to enter in cavity 1e.
Body 1, chock plug 4 in the present embodiment adopt insulant to make, first electrode 2 and the second electrode 3 adopt conductive material to make, first electrode 2 both can be directly anchored in body 1, it is also possible to the same with the second electrode 3 be arranged on inside a chock plug 4 and body 1 cavity 1e sealed again. Elastic component is an elastic component 5 adopting rubber-plastics material to make, chock plug 4 includes the tubular portion with through hole 1, second electrode 3 is plugged in through hole 1, second electrode 3 includes the body of rod 31 and the cap body 32 being connected with the body of rod 31 one end, the body of rod 31 other end can pass chock plug 4 and couple with external electrical, elastic component 5 is socketed on the body of rod 31 lateral surface, the outer end of chock plug 4 has along the radially inner gear of through hole 1 along 42, the outer face of elastic component 5 is resisted against gear along 42, and the inner face of this elastic component 5 is resisted against in cap body 32. Rubber-plastics material includes plastics, rubber, silica gel etc., during by chock plug 4 body 1 end regularly, the end face at the second electrode 3 and the cavity 1e opening 1a edge of body 1 reclines mutually, elastic component 5 produces compression, improve the sealing of the second electrode 3 and opening 1a edge end face, and the body of rod 31 can pass gear and along 42 and extend to the outside convenient of chock plug 4 and be energized to the second electrode 3; Certain second electrode 3 can also be not extend to the outside of chock plug 4, and electric connection contacts and the ends contact of the second electrode 3 outside chock plug 4 realize electrical connection. Elastic component 5 generally can make a complete round, it is also possible to is not complete round, if flexible and can grafting the second electrode 3.
The present embodiment Elastic part 5 is an independent elastic component 5, is arranged in an interference fit in through hole 1, or is arranged in through hole 1 in the mode such as bonding; Being provided with shoulder block portion 1 between elastic component 5 and cap body 32, round and elastic component 5 can be made by shoulder block portion 1 and cap body 32 is coaxially disposed, the size in shoulder block portion 1 or the diameter diameter more than the body of rod 31 and the diameter less than cap body 32. Shoulder block portion 1 can also be that incomplete round or other shapes, its size or spacial yardstick are also required to the diameter more than the body of rod 31 and the diameter less than cap body 32. One 33, shoulder block portion cap body 32 and elastic component 5 separate and make cap body 32 oppress elastic component 5 and require over shoulder block portion 1 and carry out pressure conduction, and the diameter in shoulder block portion 1 or be smaller in size than cap body 32 and elastic component 5, the bigger pressure thus produced on relatively small area of contact promotes elastic component 5 to be easier to deform upon displacement. When chock plug 4 is fixed in location structure, second electrode 3 reclines mutually with opening 1a end face, elastic component 5 is subject to being in compressive state from the pressure of the second electrode 3, produce pressure conduction by this compressive deformation and increase sealing property to the opening 1a end face of body 1 end, and produce when chock plug 4 has and upwarps on a small quantity certain to restore deformation and keep the second electrode 3 to continue to be pressed on the opening 1a end face of body 1 end.
Shoulder block portion 1 is independent parts, and shoulder block portion 1 is enclosed within the body of rod 31 between elastic component 5 and cap body 32, its material can insulate or nonisulated can; The lateral surface of elastic component 5 and the inwall of through hole 1 fit and form sealing, and the medial surface of this elastic component 5 and the lateral surface of the body of rod 31 fit and form sealing.
Convex tendon one 1d and convex tendon 2 43 can be complete annular or discontinuous annular, can reach the effect of clamping. The protrusion of convex tendon one 1d and convex tendon 2 43 can not be it is obvious that adopt chock plug 4 to fill in the size inside the connecting cylinder portion that the is sized slightly larger than 1b of body 1 part, reaches the purpose of location in an interference fit; Chock plug 4 and connecting cylinder portion 1b phase clamping, both are connected and separates all extremely convenients, moving the convenience of liquid after improving fluid injection and completing electric shock, certainly may be used without threaded mode, when adopting screw thread, chock plug 4 rotates and it can be made to be fixed on connecting cylinder portion 1b. As another kind of situation, location structure can be disposed on the grab one on chock plug 4 and be arranged on body 1 can with the buckle one of grab one phase clamping.
As shown in Figure 2, the end face at this enforcement middle tube body 1 opening 1a edge offers annular groove 1f, fill in liquid sample in cavity 1e on opening 1a, form a convex surface, when the second electrode 3 contact liq convex surface lower means of press seals opening 1a, annular groove 1f is extruded and flowed into convex surface surplus liquid, do not affect cell electroporation process in cavity 1e fluid sample.
As shown in Figure 2, the cavity 1e of body 1 is provided with ion conductive layer 1g, subsurface and first electrode 2 of ion conductive layer 1g contact, the face layer of ion conductive layer 1g can with target liq sample contact, ion conductive layer 1g separates the direct contact of cell sample and the first electrode 2, thus avoiding cell sample to be subject to the direct infringement of electrochemical reaction near the first electrode 2, ion conductive layer 1g contains the soluble-salt composition source as ion, and infiltration saline solution forms conductive ion.
Embodiment two:
As shown in Figure 4, the present embodiment is roughly the same with embodiment one, it is different in that, shoulder block portion 1 and cap body 32, the body of rod 31 are one-body molded, the inner face of elastic component 5 is resisted against on the end face in shoulder block portion 1, when in the body of rod 31 grafting with elastic component 5 time, cap body 32 and elastic component 5 are separated and by its conducting pressure by this shoulder block portion 1. The shape in shoulder block portion 1 can be annular annular, discontinuous, polygon and other shapes, its size or diameter more than the diameter of the body of rod 31 diameter less than cap body 32.
Embodiment three:
As it is shown in figure 5, the present embodiment is roughly the same with embodiment one or embodiment two, being different in that, shoulder block portion 1 is installed on elastic component 5 or shoulder block portion 1 is one-body molded with elastic component 5, and shoulder block portion 1 and cap body 32 lean. The shape in shoulder block portion 1 can be annular annular, discontinuous, polygon and other shapes, its size or diameter more than the diameter of the body of rod 31 diameter less than cap body 32.
Embodiment four:
As shown in Figure 7, the present embodiment is roughly the same with embodiment one, it is different in that, as another kind of elastic component scheme in the present embodiment, elastic component is have elastic gear along elastic component 18 along what through hole 1 extended internally out on through hole 1 inwall, gear is resisted against on the lateral surface of the body of rod 31 along elastic component 18 and forms sealing, gear is along being additionally provided with shoulder block portion 2 34 between elastic component 18 and cap body 32, shoulder block portion 2 34 is independently arranged, shoulder block portion 2 34 can make round and be coaxially disposed along elastic component 18 and cap body 32 with gear, the diameter in shoulder block portion 2 34 diameter more than the body of rod 31 and the diameter less than cap body 32. shoulder block portion 2 34 can also be incomplete round or other shapes, its size or the spacial yardstick diameter more than the body of rod 31 and the diameter less than cap body 32. this gear is identical along material and the chock plug 4 of elastic component 18, and the inwall of outside and chock plug 4 main body is connected as a single entity and one opening of intermediate formation, and the body of rod 31 of the second electrode 3 is plugged on keeps off the opening part along elastic component 18 with interference fit or the mode such as bonding. the main body of electric shock pipe 21 is for the needs of degree of accuracy, it is generally required to be made to certain intensity to prevent deformation, and the gear being connected as a single entity with chock plug 4 can adopt the mode reducing this partial intensities to realize elasticity along elastic component 18 as elastic component, this partial design such as become thickness compare other parts thinner it is thus possible to be easier to pressure deformation. this gear generally can make a complete round along elastic component 18, it is also possible to is not complete round, if the elasticity of maintaining and can grafting the second electrode 3 just passable.
Embodiment five:
As shown in Figure 8, the present embodiment and embodiment four are roughly the same, are different in that, shoulder block portion 2 34 and cap body 32 in the present embodiment, the body of rod 31 are all one-body molded, keep off the inner face along elastic component 18 and are resisted against on the end face in shoulder block portion 2 34. The shape in shoulder block portion 2 34 can be annular annular, discontinuous, polygon and other shapes, its size or diameter more than the diameter of the body of rod 31 diameter less than cap body 32.
Embodiment six:
As it is shown in figure 9, the present embodiment is roughly the same with embodiment four or five, being different in that, in the present embodiment, shoulder block portion 2 34 is one-body molded along elastic component 18 with gear, and shoulder block portion 2 34 and cap body 32 lean. The shape in shoulder block portion 2 34 can be annular annular, discontinuous, polygon and other shapes, its size or diameter more than the diameter of the body of rod 31 diameter less than cap body 32.
Embodiment seven:
As shown in Figure 10, the present embodiment is roughly the same with embodiment one, it is different in that, scheme as the third elastic component, the present embodiment Elastic part is a stage clip 6, chock plug 4 includes the tubular portion with through hole 1, second electrode 3 is arranged in through hole 1, second electrode 3 includes the body of rod 31 and the cap body 32 being connected with the body of rod 31 one end, the body of rod 31 other end can pass through the opening of chock plug 4 and couple with external electrical, stage clip 6 is socketed on the body of rod 31 lateral surface, the outer end of chock plug 4 has along the radially inner gear of through hole 1 along 42, the outer face of stage clip 6 is resisted against gear along 42, the inner face of this stage clip 6 is resisted against in cap body 32. when chock plug 4 is fixed on body 1 end, stage clip 6 can produce compression, improves the sealing property between body 1 end at the second electrode 3 and opening 1a edge.
Embodiment eight:
If Figure 11 is to, shown in 15, providing a kind of cell electroporation with electric shock pipe 21 in the present embodiment, this cell electroporation includes casing 7, is provided with fixing seat 8 in casing 7, and fixing seat 8 offers jack 821; Cell electroporation also includes the electric shock pipe 21 being plugged in this jack 821, electric shock pipe 21 includes body the 1, first electrode the 2, second electrode 3 and chock plug 4, body 1 is internal has the cavity 1e for holding target liq sample, one end of body 1 is provided with the first electrode 2, the other end of body 1 has the opening 1a being connected with cavity 1e, the working position of the first electrode 2 is connected with cavity 1e, and the edge of opening 1a has the end face of annular; Second electrode 3 is arranged in chock plug 4 and the outer end of the second electrode 3 can by the opening of chock plug 4 and external electrical connections, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, it is provided with elastic component between chock plug 4 and the second electrode 3, the lateral surface of elastic component leans with chock plug 4 or is connected, medial surface and second electrode 3 of elastic component lean, and the periphery of opening 1a has can be fixed on chock plug 4 body 1 end and make elastic component produce the location structure of compressive deformation; The inner of jack 821 has the electrode terminal 1 that can electrically connect with the first electrode 2, casing 7 is provided with the cover plate 9 for hiding jack 821 outer end, cover plate 9 is provided with the electrode terminal 2 11 electrically connected with the second electrode 3, casing 7 is additionally provided with the power module 27 electrically connected with electrode terminal 1 and electrode terminal 2 11.
This cell electroporation is in use, liquid sample can be filled in the cavity 1e of body 1, then chock plug 4 is covered, shock by electricity the cover plate 9 that then closes after pipe 21 is placed in jack 821, making electrode terminal 1 electrically connect with the first electrode 2, electrode terminal 2 11 electrically connects with the second electrode 3, then starts power pulse electric discharge, make to be formed in electric shock pipe 21 electric field, and then make extracellular material inject in cell. Owing to being additionally provided with the elastic component for strengthening the active force between the second electrode 3 and opening 1a end face in chock plug 4, therefore can avoid, between the second electrode 3 and opening 1a, gap occurs; Further, when the phenomenon that slightly upwarps occurs in chock plug 4, elastic component also can do certain recovery deformation, it is ensured that the second electrode 3 still reclines mutually with body 1 end of opening 1a end face, thus avoiding extraneous air to enter cavity 1e; Also make electric shock pipe 21 bubble that oneself electrochemical reaction internal produces among electric shock process suffer restraints simultaneously. To sum up, electric shock pipe 21 in this cell electroporation can be effectively improved the sealing property between the second electrode 3 and opening 1a end face, turn thus avoiding air extraneous in electric shock set-up procedure to enter formation aeration electricity in electric shock pipe 21 cavity 1e, and reduce the bubble of the electrochemical reaction generation impact on electric current in electric shock process, thus improving the electroporation performance of this cell electroporation.
As shown in Figure 11, Figure 12 and Figure 13, fixing seat 8 includes pedestal 81 and clamping cylinder 82, the jack 821 offered in clamping cylinder 82, a slot 811 is offered in pedestal 81, clamping cylinder 82 is inserted in slot 811 and both detachably interconnectings, the bottom land of the bottom of clamping cylinder 82 and slot 811 reclines mutually, and the top of clamping cylinder 82 is provided with at least one lever 822.
As shown in figure 12, an inner chamber 12 it is further opened with in pedestal 81, the bottom land of slot 811 offers through hole 2 13, the two ends of through hole 2 13 are connected with jack 821 and inner chamber 12 respectively, inner chamber 12 is provided with spring base 14 and spring 15, the inner of spring 15 is fixed on spring base 14, and the outer end of spring 15 is connected with the inner of electrode terminal 1, and the outer end of electrode terminal 1 can be passed through hole 2 13 and be inserted in jack 821. After design has spring base 14 and spring 15, when cover plate 9 covers jack 821 opening 1a, first electrode 2 and the second electrode 3 can electrically connect with electrode terminal 1 and electrode terminal 2 11 respectively, spring 15 during electric shock pipe 21 pushed down by cover plate 9, and by the compression bigger extra pressure of generation, this pressure is transmitted to cavity 1e opening 1a via the second electrode 3, strengthens the sealing of the second electrode 3 and cavity 1e opening 1a. Due to electrochemical reaction electrolysis meeting creating pockets of air in cell electric shock process, when between the second electrode 3 and cavity 1e opening 1a, pressure is relatively larger, these electrochemistry bubbles can be compressed and be reduced it to the impact of CURRENT DISTRIBUTION in electric shock process, thus improving cell electroporation effect. Cell has shocked by electricity back shroud 9 when opening, and electric shock pipe 21 can eject a part, convenient pickup under the effect of spring 15 from jack 821.
The inner of cover plate 9 is articulated with in casing 7, the outer end of cover plate 9 is provided with buckle 2 16, being additionally provided with in casing 7 can with the grab 2 17 of buckle 2 16 phase clamping, cover plate 9 clamping cylinder 82 is made up of the light transmittance material more than 50%, the sensor 29 for detecting electric shock pipe 21 change in displacement it is provided with in pedestal 81 adnexa, micro-control unit 25 it is provided with in casing 7, micro-control unit 25 electrically connects with sensor 29, being additionally provided with the display lamp 28 electrically connected with micro-control unit 25 in pedestal 81, display lamp 28 also electrically connects with power module 27. Display lamp 28 is except having instruction electric shock pipe 21 connection status effect, can also there is illuminating effect simultaneously, the light source such as LED etc. that can adopt higher brightness illuminates electric shock pipe 21, sensor 29 can trigger switch for mechanicalness, and the depression bar linked with it when electrode terminal 1 is subjected to displacement triggers switch; Sensor 29 can also be photoswitch, when electrode terminal 1 is subjected to displacement, light path is produced impact and triggers photoswitch; Sensor 29 can also Hall switch etc., when shocking by electricity pipe 21 or electrode terminal 1 triggers when being subjected to displacement.
Display lamp 28 is arranged in the casing 7 near electric shock pipe 21 jack 821, and light illuminates electric shock pipe 21 nearby. as other situations, display lamp 28 can also be arranged on fixing seat 8, and fixing seat 8 can open horizontal through hole to install display lamp 28. clamping cylinder 82 can be made by transparent material, and the display lamp 28 on fixing seat 8 can pass through clamping cylinder 82 and illuminate electric shock pipe 21 from the side, is formed and compares visual effect clearly. being provided with a display screen 26 in casing 7, be provided with sampler 24 in casing 7, micro-control unit 25 electrically connects with display screen 26 and power module 27, and sampler 24 electrically connects with electric shock pipe 21 and micro-control unit 25 respectively. power module 27 can produce the electric pulse that cell electroporation needs, display screen 26 is used for display instrument and experiment information and can show experimental implementation interface, micro-control unit 25 can control power module 27 and display screen 26, micro-control unit 25 includes programmable single-chip microcomputer and other microprocessors etc., sampler 24 can gather the signal of telecommunication in cell electroporation process, including voltage or current signal, sampler 24 includes the electronic components such as resistance, the signal of telecommunication processes via micro-control unit 25, relevant parameter can be shown on the display 26 with the form of numerical value or oscillogram.
Shoulder block portion 1 in above example and shoulder block portion 2 34 may each be complete ring-type, it is possible to use some sections of arcs compositions, the simple transformation of its planform is all substantially identical with the technical program.
Specific embodiment described in literary composition is only to present invention spirit explanation for example. Described specific embodiment can be made various amendment or supplements or adopt similar mode to substitute by those skilled in the art, but without departing from the spirit of the present invention or surmount the scope that appended claims is defined.

Claims (25)

1. an electric shock pipe, described electric shock pipe includes body (1), first electrode (2), second electrode (3) and chock plug (4), described body (1) is internal has the cavity (1e) for holding target liq sample, it is characterized in that, one end of described body (1) is provided with described first electrode (2), the other end of described body (1) has the opening (1a) being connected with described cavity (1e), the working position of described first electrode (2) is connected with described cavity (1e), the edge of described opening (1a) has the end face of annular, described second electrode (3) is arranged in chock plug (4) and the outer end of described second electrode (3) can be coupled with external electrical by the opening of described chock plug (4), the inner face of described second electrode (3) can fit with the annular end face at opening (1a) edge, the elastic component (5) being connected with the second electrode (3) it is provided with in described chock plug (4), the outside of described elastic component (5) is connected with described chock plug (4), the inner side of described elastic component is connected with described second electrode (3), the periphery of described opening (1a) has can be fixed on chock plug (4) body (1) end and make described elastic component produce the location structure of compressive deformation.
2. electric shock pipe according to claim 1, it is characterized in that, described chock plug (4) includes the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) includes the body of rod (31) and cap body (32), the described body of rod (31) one end is connected with described cap body (32), the described body of rod (31) other end can pass through the opening of described chock plug (4) and couple with external electrical, described elastic component (5) is socketed on the body of rod (31) lateral surface, the outer end of described chock plug (4) has along radially inner gear edge (42) of through hole one (41), the outer face of described elastic component (5) is resisted against gear on (42), the inner face of this elastic component (5) is resisted against in cap body (32).
3. electric shock pipe according to claim 2, it is characterized in that, shoulder block portion one (33) it is provided with, the diameter being sized larger than the body of rod (31) in described shoulder block portion one (33) and the diameter less than cap body (32) between described elastic component (5) and described cap body (32).
4. electric shock pipe according to claim 3, it is characterized in that, described shoulder block portion one (33) is installed in described cap body (32) and is above connected with described cap body (32), the body of rod (31), and the inner face of described elastic component (5) is resisted against on the end face in described shoulder block portion one (33).
5. electric shock pipe according to claim 3, it is characterized in that, described shoulder block portion one (33) is installed in described elastic component (5) and is above connected with described elastic component (5), and described shoulder block portion one (33) leans with described cap body (32).
6. electric shock pipe according to claim 1, it is characterized in that, described chock plug (4) includes the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) includes the body of rod (31) and cap body (32), the described body of rod (31) one end is connected with described cap body (32), the described body of rod (31) other end can pass through the opening of described chock plug (4) and couple with external electrical, described elastic component (5) is for having elastic gear along elastic component (18) along what through hole one (41) extended internally out on described through hole one (41) inwall, described gear is resisted against on the lateral surface of the described body of rod (31) along elastic component (18).
7. electric shock pipe according to claim 6, it is characterized in that, described gear along being provided with shoulder block portion two (34) between elastic component (18) and described cap body (32), the diameter being sized larger than the body of rod (31) in described shoulder block portion two (34) and the diameter less than cap body (32).
8. electric shock pipe according to claim 7, it is characterized in that, described shoulder block portion two (34) is installed in described cap body (32) and is above connected with described cap body (32), the body of rod (31), and described gear is resisted against on the end face in described shoulder block portion two (34) along the inner face of elastic component (18).
9. electric shock pipe according to claim 7, it is characterized in that, described shoulder block portion two (34) is installed in described gear and is connected along elastic component (18) with described gear along elastic component (18) is upper, and described shoulder block portion two (34) leans with described cap body (32).
10. electric shock pipe according to claim 1, it is characterized in that, described elastic component (5) is a stage clip (6), described chock plug (4) includes the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) includes the body of rod (31) and cap body (32), the described body of rod (31) one end is connected with described cap body (32), the described body of rod (31) other end can pass through the opening of described chock plug (4) and couple with external electrical, described stage clip (6) is socketed on the body of rod (31) lateral surface, the outer end of described chock plug (4) has along radially inner gear edge (42) of through hole one (41), the outer face of described stage clip (6) is resisted against gear on (42), the inner face of this stage clip (6) is resisted against in cap body (32).
11. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, described location structure includes the connecting cylinder portion (1b) being connected as a single entity with the end of described body (1), described connecting cylinder portion (1b) offers the barrel chamber (1c) for described chock plug (4) grafting, having convex tendon one (1d) in the cavity wall of described barrel chamber (1c), the lateral surface of described chock plug (4) has can with the convex tendon two (43) of described convex tendon one (1d) phase clamping.
12. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, described location structure includes the grab one being arranged on described chock plug (4), and described body (1) has can with the buckle one of described grab one phase clamping.
13. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterised in that the end face at described opening (1a) edge offers annular groove (1f).
14. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterised in that described chock plug (4) is connected by a flexible connecting member (30) with described body (1).
15. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, the outside of described elastic component (5) and described chock plug (4) fit and form sealing, and the lateral surface of the inner side of described elastic component (5) and described second electrode (3) fits and forms sealing.
16. according to the electric shock pipe described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, the cavity (1e) of described body (1) is provided with ion conductive layer (1g), the subsurface of described ion conductive layer (1g) and the first electrode (2) contact, the face layer of described ion conductive layer (1g) can with target liq sample contact.
17. a cell electroporation with electric shock pipe, described cell electroporation includes casing (7), it is provided with fixing seat (8) in described casing (7), described fixing seat (8) offers jack (821), it is characterized in that, described cell electroporation also includes the electric shock pipe (21) being plugged in this jack (821), described electric shock pipe (21) includes body (1), first electrode (2), second electrode (3) and chock plug (4), described body (1) is internal has the cavity (1e) for holding target liq sample, one end of described body (1) is provided with described first electrode (2), the other end of described body (1) has the opening (1a) being connected with described cavity (1e), the working position of described first electrode (2) is connected with described cavity (1e), the edge of described opening (1a) has the end face of annular, described second electrode (3) is arranged in chock plug (4) and the outer end of described second electrode (3) can by the opening of described chock plug (4) and external electrical connections, the inner face of described second electrode (3) can fit with the annular end face at opening (1a) edge, the elastic component (5) being connected with the second electrode (3) it is provided with in described chock plug (4), the outside of described elastic component (5) is connected with described chock plug (4), the inner side of described elastic component is connected with described second electrode (3), the periphery of described opening (1a) has can be fixed on chock plug (4) body (1) end and make described elastic component produce the location structure of compressive deformation, the inner of described jack (821) has the electrode terminal one (10) that can electrically connect with described first electrode (2), described casing (7) is provided with the cover plate (9) for hiding jack (821) outer end, described cover plate (9) is provided with the electrode terminal two (11) electrically connected with described second electrode (3), described casing (7) is additionally provided with the power module (27) electrically connected with described electrode terminal one (10) and electrode terminal two (11).
18. the cell electroporation with electric shock pipe according to claim 17, it is characterized in that, described fixing seat (8) includes pedestal (81) and clamping cylinder (82), described jack (821) is offered in described clamping cylinder (82), offering a slot (811) in described pedestal (81), described clamping cylinder (82) is inserted in slot (811) and both detachably interconnectings.
19. the cell electroporation with electric shock pipe according to claim 18, it is characterised in that described clamping cylinder (82) is made up of the light transmittance material more than 50%.
20. the cell electroporation with electric shock pipe according to claim 18, it is characterized in that, the bottom land of the bottom of described clamping cylinder (82) and described slot (811) reclines mutually, and the top of described clamping cylinder (82) is provided with at least one lever (822).
21. the cell electroporation with electric shock pipe according to claim 18, it is characterized in that, an inner chamber (12) it is further opened with in described pedestal (81), the bottom land of described slot (811) offers through hole two (13), the two ends of described through hole two (13) are connected with described jack (821) and inner chamber (12) respectively, described inner chamber (12) is provided with spring base (14) and spring (15), the inner of described spring (15) is fixed on spring base (14), the outer end of described spring (15) is connected with the inner of described electrode terminal one (10), the outer end of described electrode terminal one (10) can be passed described through hole two (13) and be inserted in described jack (821).
22. the cell electroporation with electric shock pipe according to claim 18 or 19 or 20 or 21, it is characterized in that, described cover plate (9) is made up of the light transmittance material more than 50%, the sensor (29) for detecting electric shock pipe (21) change in displacement it is provided with in described pedestal (81), the micro-control unit (25) being connected with sensor (29) it is provided with in described casing (7), display lamp (28) it is additionally provided with in described casing (7), described display lamp (28) is connected with described power module (27), the signal of described sensor (29) can pass to described micro-control unit (25) and be used for controlling display lamp (28).
23. the cell electroporation with electric shock pipe according to claim 22, it is characterized in that, described casing (7) is provided with a display screen (26), sampler (24) it is provided with in described casing (7), described micro-control unit (25) is connected with display screen (26) and power module (27), and described sampler (24) is connected with electric shock pipe (21) and micro-control unit (25) respectively.
24. the cell electroporation with electric shock pipe according to claim 23, it is characterized in that, described samplers sample to the signal of telecommunication can pass to micro-control unit (25) and show with the form of wavy curve on display screen (26).
25. the cell electroporation with electric shock pipe according to claim 17 or 18 or 19 or 20 or 21, it is characterized in that, the inner of described cover plate (9) is articulated with in casing (7), the outer end of described cover plate (9) is provided with buckle two (16), and described casing (7) is additionally provided with can with the grab two (17) of described buckle two (16) phase clamping.
CN201410722470.9A 2014-12-02 2014-12-02 A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe Active CN104403943B (en)

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CN201410722470.9A CN104403943B (en) 2014-12-02 2014-12-02 A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe
JP2017530204A JP6629858B2 (en) 2014-12-02 2015-12-02 Electric shock pipe and electroporation apparatus having the electric shock pipe
PCT/IB2015/059297 WO2016088062A1 (en) 2014-12-02 2015-12-02 An electric shock pipe and cell electroporator having electric shock pipe
US15/532,182 US10472601B2 (en) 2014-12-02 2015-12-02 Shock tube and cell electroporation device with the shock tube
EP15864958.2A EP3228695A4 (en) 2014-12-02 2015-12-02 An electric shock pipe and cell electroporator having electric shock pipe
US16/586,989 US11965149B2 (en) 2014-12-02 2019-09-29 Shock tube and cell electroporation device with the shock tube
JP2019219959A JP6838125B2 (en) 2014-12-02 2019-12-04 An electric shock pipe and an electroporation device having the electric shock pipe

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US10472601B2 (en) 2014-12-02 2019-11-12 Jian Chen Shock tube and cell electroporation device with the shock tube
CN110437999A (en) * 2019-07-15 2019-11-12 广州蛟龙细胞医药科技有限公司 A kind of electroporation
CN114196533A (en) * 2021-11-01 2022-03-18 上海盟德生物科技有限公司 Microchannel cell electrotransformation instrument

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