CN104403943A - Electroporation cuvette and cell electrotransfection device with same - Google Patents

Electroporation cuvette and cell electrotransfection device with same Download PDF

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Publication number
CN104403943A
CN104403943A CN201410722470.9A CN201410722470A CN104403943A CN 104403943 A CN104403943 A CN 104403943A CN 201410722470 A CN201410722470 A CN 201410722470A CN 104403943 A CN104403943 A CN 104403943A
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CN
China
Prior art keywords
electrode
electric shock
elastic component
chock plug
shock pipe
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Granted
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CN201410722470.9A
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Chinese (zh)
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CN104403943B (en
Inventor
陈剑
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Taizhou Sairui TREK Biological Technology Co.,Ltd.
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Individual
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Priority to CN201410722470.9A priority Critical patent/CN104403943B/en
Publication of CN104403943A publication Critical patent/CN104403943A/en
Priority to JP2017530204A priority patent/JP6629858B2/en
Priority to EP15864958.2A priority patent/EP3228695A4/en
Priority to PCT/IB2015/059297 priority patent/WO2016088062A1/en
Priority to US15/532,182 priority patent/US10472601B2/en
Application granted granted Critical
Publication of CN104403943B publication Critical patent/CN104403943B/en
Priority to US16/586,989 priority patent/US11965149B2/en
Priority to JP2019219959A priority patent/JP6838125B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube

Abstract

The invention provides an electroporation cuvette and a cell electrotransfection device with the electroporation cuvette and belongs to the technical field of biomedical instruments and equipment. The electroporation cuvette and the cell electrotransfection device solve the technical problems such as poor cell electrotransfection stability at present. The electroporation cuvette comprises a cuvette body, a first electrode, a second electrode and a plug, wherein the cuvette body is internally provided with a cavity for accommodating a target liquid sample; the first electrode is arranged at one end of the cuvette body; the second electrode is arranged in the plug; the outer end of the second electrode can be electrically connected with the exterior via an opening of the plug; the plug is internally provided with an elastic piece connected with the second electrode; the outer side of the elastic piece is connected with the plug; and the inner side of the elastic piece is connected with the second electrode. The invention further provides the cell electrotransfection device where the electroporation cuvette can be placed. The electroporation cuvette can effectively improve sealability between the second electrode and the opening, thereby preventing outside air from entering the cavity when the liquid sample is added, and the cell electrotransfection device is convenient to operate and facilitates observation.

Description

The cell electroporation of electric shock pipe is managed and had to a kind of electric shock
Technical field
The invention belongs to biological medicine plant and instrument technical field, relate to a kind of electric shock and manage and have the cell electroporation of electric shock pipe.
Background technology
Cell electroporation (turning also known as cell electrotransfection or cell electricity) be utilize electricimpulse to introduce in cell cannot the macromolecular technology of permeates cell membranes.Electroporation is a kind of extensive employing and obtains the method for test cell line and gene therapy of strong preference.When applying highfield, cytolemma temporarily becomes porous character and can through foreign material, such as macromole.Cytolemma electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulsed voltage, pulse duration, number of pulses and other experiment conditions.
Equipment at present for cell electroporation mainly contains cell electroporation, pole cup etc.China patent CN 1965079B discloses a kind of electroporation device with long hollow member, this electroporation device comprises long hollow member, to provide uniform electric field in electroporation process, wherein particularly, perform electroporation be by be full of in hollow member comprise cell and will inject cell the liquid sample of material after, apply electricimpulses by pair of electrodes from long hollow member two ends.
Applicant once declared one section of " method and apparatus of electroporation " METHODS AND DEVICES FOR ELECTROPORATION by name; The United States Patent (USP) of US 2013/0052711 A1, a kind of electric shock pipe is described in this patent, its effect adds containing cell and the liquid sample of material that will inject cell in pipe, at the upper/lower terminal of electric shock pipe, top electrode and lower electrode are set respectively, be connected with lower electrode with top electrode by cell electroporation, thus make to form electric field in electric shock pipe, and then extracellular material is injected in cell.And liquid sample being filled in after in electric shock pipe, before starting electric shock, need in electric shock pipe to have prevented air residual from forming aeration distribution of current.In that patent, applicant is designed with an annular recesses be connected with electric shock tube chamber on the tube wall of electric shock pipe, experimenter is sustainable to injection liquid sample body in electric shock tube chamber until after fluid surface protrusion electric shock tube chamber, to contact until be pressed onto the upper edge of the tube chamber that shocks by electricity under the polar cap that power on liquid convex surface again, form the sealing to liquid inside cavity, and the liquid overflowed on a small quantity flows in annular recesses.This design generally can prevent having residual air to affect experiment in electric shock pipe.
But applicant finds in constantly putting into practice, the electric shock pipe in above-mentioned patent is higher to the precise requirements manufactured, and the requirement of experimenter's operation is simultaneously also higher.If accurate not in manufacture, easily cause the poor sealing on edge on electrode and cavity, may under electrode cap after generating electrodes upwarp and again leave edge on cavity and cause extraneous air to reenter the situation of cavity.If also easily cause there is residual air bubble in pipe in addition when operator's improper use, affect the effect of cell electroporation.Scientific experiment is very high to the reliability requirement of plant and instrument, and therefore, above-mentioned electric shock pipe needs on manufacturing, increase the design that some improve reliability, thus improves the reliability of experimenter's operation.
Summary of the invention
The present invention be directed to the problems referred to above that existing technology exists, provide a kind of electric shock pipe.Technical problem to be solved by this invention is: how by the sealing property between raising second electrode and open end-face, thus avoids extraneous air to enter in cavity.
The present invention be directed to the problems referred to above that existing technology exists, additionally provide a kind of cell electroporation with electric shock pipe, technical problem to be solved by this invention is: the electroporation performance how improving this cell electroporation.
First object of the present invention realizes by following technical proposal:
A kind of electric shock pipe, described electric shock pipe comprises body, the first electrode, the second electrode and chock plug, described tubular body has the cavity for holding target liq sample, it is characterized in that, one end of described body is provided with described first electrode, the other end of described body has the opening be connected with described cavity, and the working position of described first electrode is connected with described cavity, and the edge of described opening has the end face of annular; Described second electrode is arranged in chock plug and the outer end of described second electrode and can be electrically connected by the opening of described chock plug and outside, the inner face of described second electrode can fit with the annular end face of edge of opening, the elastic component be connected with the second electrode is provided with in described chock plug, the outside of described elastic component is connected with described chock plug, the inner side of described elastic component is connected with described second electrode, and the periphery of described opening has can be fixed on body end by chock plug and make described elastic component produce the locating structure of compressive set.
Its principle of work is as follows: this electric shock pipe in use, the liquid sample of the material comprising cell and cell will be injected can be filled in forming a liquid convex surface to cavity, then make chock plug be fixed on body end by fixed sturcture and make the elastic component between chock plug and the second electrode produce compressive set, now the second electrode is crushed on open end-face, first electrode and the second electrode all with liquid communication in cavity, the first electrode is made to be connected with the pulse power with the second electrode again, electric discharge makes to form electric field in electric shock pipe, cytolemma is made to have certain permeability, thus the target substance in liquid sample is entered in cell.When chock plug is fixed on body end, elastic component produces compressive set, can avoid occurring gap between the second electrode and opening, improve the sealing property between the second electrode and open end-face, thus avoid air to enter liquid sample in cavity, and, when slightly upwarping appears in chock plug time, elastic component also can do certain recovery deformation, ensure that the second electrode still reclines mutually with the end face of edge of opening, thus make the liquid sample in cavity still can not produce air bubble when experimenter's application of sample operates some deviation; In sum, this electric shock pipe can improve the sealing property between the second electrode and opening effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity.
Locating structure in the application is directly for elastic component provides the stable support in outer end and location, elastic component is made to be in stable compressed state between chock plug and the second electrode, such elastic component can apply stable elastic force to the second electrode, make the laminating between the second electrode and the open end-face of body more closely with stable, thus the sealing effectiveness enhanced between the second electrode and the open end-face of body, locating structure and elastic component interwoveness, jointly solve the technical problem of " how strengthening the sealing stability between the second electrode and body ".
It should be noted that the body in the application, chock plug adopt insulating material to make, the first electrode and the second electrode adopt electro-conductive material to make, and this part is prior art, and not in this to go forth for the material of embody rule.In addition, the first electrode both can directly be fixed in body, also equally with the second electrode can be arranged on inside a chock plug and seal body cavity.
In above-mentioned one electric shock pipe, described chock plug comprises the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode comprises the body of rod and cap body, one end and the described cap body of the described body of rod are connected, the described body of rod the other end can be electrically connected by the opening of described chock plug and outside, described elastic component is socketed on body of rod outer side, the outer end of described chock plug has along the radially inner gear edge of through hole one, the outer face of described elastic component is resisted against gear along upper, and the inner face of this elastic component is resisted against on cap body.Described elastic component adopts rubber-plastics material to make, and rubber-plastics material comprises plastics, rubber, silica gel etc.When chock plug is fixed on body end, the second electrode reclines mutually with the end face at the cavity hatch edge of body, and elastic component produces compression set, improves the stopping property of the second electrode and edge of opening end face.The body of rod of described second electrode can keep off opening and the external electrical connections on edge by chock plug, the body of rod of the second electrode can extend to the outside of chock plug to the second electrifying electrodes; The described body of rod also can not extend to the outside of chock plug, and the electric connection contacts of chock plug outside also can be put in chock plug by the opening of chock plug and be electrically connected with the second electrode.Elastic component generally can make round, also can be incomplete round even other shapes, as long as flexible and can grafting second electrode.In above-mentioned one electric shock pipe, described elastic component can be an independently elastic component, is arranged in an interference fit in through hole one, or is arranged in through hole one in the mode such as bonding and is connected with described chock plug.Described elastic component can shrink-fit or bonding mode be socketed on the body of rod of described second electrode.
In above-mentioned one electric shock pipe, shoulder block portion one is provided with between described elastic component and described cap body, described shoulder block portion one is between described elastic component and described cap body, and the size in described shoulder block portion one or spacial yardstick are greater than the diameter of the body of rod and are less than the diameter of cap body.Shoulder block portion one separates cap body and elastic component and makes cap body oppress elastic component and can only carry out pressure conduction by shoulder block portion one, and the diameter in shoulder block portion one or size are less than cap body and elastic component, produce thus and impel elastic component more easily deformational displacement to occur compared with the larger pressure on small area of contact.When chock plug is fixed in locating structure, second electrode reclines mutually with open end-face, elastic component is subject to the pressure from the second electrode and is in compressed state, produce pressure conduction by this compressive set and increase sealing property to the open end-face of body end, and chock plug have upwarp on a small quantity in produce certain recovery deformation and keep the second electrode to continue to be pressed on the open end-face of body end.
In above-mentioned one electric shock pipe, described shoulder block portion one can be independently parts, such as size is less than an independent round shoulder block of elastic component and cap body, and shoulder block portion one is enclosed within the body of rod between elastic component and cap body, its material can be insulation or nonisulated can.
In above-mentioned one electric shock pipe, as the second situation, described shoulder block portion one is installed on described cap body or described shoulder block portion one and described cap body, the body of rod are one-body molded, and the inner face of described elastic component is resisted against on the end face in described shoulder block portion one.When in body of rod grafting and elastic component time, cap body and elastic component are separated by this shoulder block portion one and by its conducting pressure.
In above-mentioned one electric shock pipe, as the third situation, described shoulder block portion one be installed on described elastic component or described shoulder block portion one one-body molded with elastic component, described shoulder block portion one leans with described cap body.
In above-mentioned one electric shock pipe, as in another kind of elastic component scheme, the mode of connection of elastic component and described chock plug is for be directly connected, described chock plug comprises the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode comprises the body of rod and cap body, one end and the described cap body of the described body of rod are connected, the described body of rod the other end can be electrically connected by the opening of described chock plug and outside, described elastic component be described through hole one inwall extends internally out along through hole one there is elastic gear along elastic component, described gear is resisted against on the outer side of the described body of rod along elastic component.This gear is identical with chock plug along the material of elastic component, and the inwall of outside and chock plug main body is connected as a single entity and intermediate formation opening, and the body of rod of the second electrode is plugged on the opening part of gear along elastic component with shrink-fit or the mode such as bonding.The main body of electric shock pipe, for the needs of tolerance range, generally needs to make have certain intensity to prevent distortion; And the mode reducing this partial intensities can be adopted with the gear that chock plug is connected as a single entity to realize elasticity as elastic component along elastic component, such as this partial design become thickness to compare other parts thinner thus more easily pressure deformation can occur.This gear generally can make a round along elastic component, may not be round, if the elasticity of maintaining and can grafting second electrode just passable.
In above-mentioned one electric shock pipe, as the first situation, described gear is provided with shoulder block portion two along between elastic component and described cap body, and the size in described shoulder block portion two or spacial yardstick are greater than the diameter of the body of rod and are less than the diameter of cap body.This shoulder block portion two can be independently back-up ring.
In above-mentioned one electric shock pipe, the second situation be shoulder block portion two be installed on described cap body or described shoulder block portion two and described cap body, the body of rod all one-body molded, described gear is resisted against on the end face in described shoulder block portion two along the inner face of elastic component.
In above-mentioned one electric shock pipe, it is one-body molded along elastic component with gear along on elastic component or described shoulder block portion two that the third situation is that shoulder block portion two is installed in described gear, and described shoulder block portion two leans with described cap body.
In above-mentioned one electric shock pipe, as the scheme of the third kind of elastic component, described elastic component is a stage clip, described chock plug comprises the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode comprises the body of rod and cap body, one end and the described cap body of the described body of rod are connected, the described body of rod the other end can be electrically connected by the opening of described chock plug and outside, described compression spring sleeve is connected on body of rod outer side, the outer end of described chock plug has along the radially inner gear edge of through hole one, the outer face of described stage clip is resisted against gear along upper, the inner face of this stage clip is resisted against on cap body.When chock plug is fixed on body end, stage clip can produce compression set, the sealing property between the body end of improving the second electrode and edge of opening.
In above-mentioned one electric shock pipe, described locating structure comprises the connecting cylinder portion be connected as a single entity with the end of described body, described connecting cylinder portion offers the cylinder chamber for described chock plug grafting, the chamber wall in described cylinder chamber has convex tendon one, the outer side of described chock plug has can with the convex tendon two of described convex tendon one phase clamping.Described convex tendon one and convex tendon two can be complete annular or discontinuous annular is not even annular, can reach the effect of clamping.Convex tendon one can not be clearly with the protrusion of convex tendon two, and the size adopting chock plug to fill in body portion is a bit larger tham the size inside connecting cylinder portion, reaches the object of location in an interference fit; Chock plug and the phase clamping of connecting cylinder portion, both are connected and is separated all very convenient, improve fluid injection and move the convenience of liquid after completing electric shock, certainly also can adopt the mode be threaded, when adopting screw thread, chock plug rotation can make it be fixed on connecting cylinder portion.
Above-mentioned one electric shock pipe in, described locating structure comprises the grab one be arranged on described chock plug, described body has can with the buckle one of described grab one phase clamping.
In above-mentioned one electric shock pipe, the end face of described edge of opening offers annular recesses.This electric shock pipe in use, liquid sample can be filled in cavity, until liquid sample forms a convex surface on opening, press down sealed open by the second electrode contact liquid convex surface again, ensure in cavity without residual air, and the surplus liquid formed required for convex surface flows into annular recesses, do not affect cell electroporation process in cavity liquid sample.
In above-mentioned one electric shock pipe, described chock plug is connected by a flexible connecting member with described body.The two ends of flexible connecting member are connected with body with chock plug respectively, chock plug was both connected with body, relatively can swings by body again, avoiding because losing chock plug because of carelessness, the plastic thickness of described flexible piece makes smaller, can realize the flexibility that its wide-angle is bending.
In above-mentioned one electric shock pipe, the outside of described elastic component and described chock plug fit and form sealing, and the inner side of described elastic component and the outer side of the second electrode fit and form sealing.
In above-mentioned one electric shock pipe, be provided with ion conductive layer in the cavity of described body, the subsurface of described ion conductive layer and the first electrode contact, the upper aspect of described ion conductive layer can with target liq sample contact.As above design, cell sample can be separated contact with the direct of the first electrode, thus avoid cell sample to be subject to the direct infringement of the first ate electrode electrochemical reaction, ion conductive layer contains the source of soluble salt composition as ion, gel or semi-solid state can be formed containing gelatinous mass such as agarose, agar, polyacrylamide, colloid albumen etc., or can infiltrate that salts solution formed containing porous solid can the state of ionic conduction.
Second object of the present invention realizes by following technical proposal:
Have a cell electroporation for electric shock pipe, described cell electroporation comprises casing, is provided with permanent seat in described casing, and described permanent seat offers jack; It is characterized in that, described cell electroporation also comprises the electric shock pipe be plugged in this jack, described electric shock pipe comprises body, the first electrode, the second electrode and chock plug, described tubular body has the cavity for holding target liq sample, one end of described body is provided with described first electrode, the other end of described body has the opening be connected with described cavity, and the working position of described first electrode is connected with described cavity, and the edge of described opening has the end face of annular; Described second electrode is arranged in chock plug and the outer end of described second electrode and can be electrically connected by the opening of described chock plug and outside, the inner face of described second electrode can fit with the annular end face of edge of opening, the elastic component be connected with the second electrode is provided with in described chock plug, the outer side of described elastic component is connected with described chock plug, the inner side of described elastic component is connected with described second electrode, and the periphery of described opening has can be fixed on body end by chock plug and make described elastic component produce the locating structure of compressive set; The inner of described jack has the electrode terminal one that can be electrically connected with described first electrode, described casing is provided with the cover plate for hiding jack outer end, described cover plate is provided with the electrode terminal two that can be electrically connected with described second electrode, described casing is also provided with the power module be electrically connected with described electrode terminal one and electrode terminal two.
Its principle of work is as follows: this cell electroporation in use, liquid sample can be filled in the cavity of body, then chock plug is covered, then close after electric shock pipe is placed in jack cover plate, electrode terminal one is electrically connected with the first electrode, and electrode terminal two is electrically connected with the second electrode, then starts power pulse electric discharge, make to form electric field in electric shock pipe, and then extracellular material is injected in cell.Owing to being also provided with the elastic component for strengthening the reactive force between the second electrode and open end-face in chock plug, therefore can avoid occurring gap between the second electrode and opening; Further, when the phenomenon that slightly upwarps appears in chock plug, elastic component also can do certain recovery deformation, ensures that the second electrode still reclines mutually with the body end of open end-face, thus avoids extraneous air to enter cavity; Also make the electric shock pipe bubble that oneself electrochemical reaction inner produces among electric shock process suffer restraints simultaneously.In sum, electric shock pipe in this cell electroporation can improve the sealing property between the second electrode and open end-face effectively, thus avoid entering in electric shock tube chamber at the air extraneous in set-up procedure that shocks by electricity forming aeration electricity turn, and reduce the bubble of electrochemical reaction generation in electric shock process to the impact of electric current, thus improve the electroporation performance of this cell electroporation.
A kind ofly to have in the cell electroporation of electric shock pipe above-mentioned, described permanent seat comprises pedestal and clamping cylinder, offers described jack in described clamping cylinder, offers a slot in described pedestal, and described clamping cylinder to be inserted in slot and both detachably interconnectings.Clamping cylinder and slot removably connect, then the diameter of the pipe that can shock by electricity according to difference, change the clamping cylinder of corresponding jack pore size, can improve the versatility of this cell electroporation.
A kind ofly to have in the cell electroporation of electric shock pipe above-mentioned, the material that described clamping cylinder is greater than 50% by transmittance is made.
A kind ofly to have in the cell electroporation of electric shock pipe above-mentioned, the bottom of described clamping cylinder reclines mutually with the bottom land of described slot, and the top of described clamping cylinder is provided with at least one lever.After being designed with lever, handled easily person installs and removes clamping cylinder.In order to adapt to shorter electric shock pipe, also metal electrode extension device can be installed below clamping cylinder, contact with electrode terminal one below this metal enhance part, after shorter electric shock pipe inserts clamping cylinder, the first electrode realizes being electrically connected by metal enhance part with electrode terminal one, supplements the deficiency of electric shock pipe height like this.
A kind ofly have in the cell electroporation of electric shock pipe above-mentioned, an inner chamber is also offered in described pedestal, the bottom land of described slot offers through hole two, the two ends of described through hole two respectively with described jack and intracavity inter-connection, spring support and spring is provided with in described inner chamber, the inner of described spring is fixed on spring support, and the outer end of described spring is connected with the inner of described electrode terminal one, and the outer end of described electrode terminal one can be passed described through hole two and be inserted in described jack.After being designed with spring support and spring, when cover plate covers jack openings, first electrode and the second electrode can be electrically connected with electrode terminal one and electrode terminal two respectively, when electric shock pipe pushed down by cover plate, described spring is compressed the larger extra pressure of generation and this pressure is transmitted to described cavity hatch via the second electrode, strengthens the stopping property of the second electrode and cavity hatch.Due to electrochemical reaction electrolysis meeting creating pockets of air in cell electric shock process, when between the second electrode and cavity hatch, pressure ratio is larger, these electrochemistry bubbles can be compressed and reduce its impact on distribution of current in electric shock process, thus improve cell electroporation effect.Cell has shocked by electricity back shroud when opening, and electric shock pipe can eject a part under the action of the spring from jack, convenient pickup.
A kind ofly have in the cell electroporation of electric shock pipe above-mentioned, the material that described cover plate is greater than 50% by transmittance is made, described pedestal annex is provided with the sensor for detecting electric shock pipe change in displacement, the micro-control unit be connected with sensor electrical is provided with in described casing, also pilot lamp is provided with in described casing, described pilot lamp is also connected with described power module, and the signal of described sensor can pass to described micro-control unit and be used for controlling pilot lamp.Pilot lamp is except having the effect of instruction electric shock pipe connection state, also illuminating effect can be had simultaneously, the pilot lamp of higher brightness such as LED etc. can be adopted to illuminate electric shock pipe, and sensor can be mechanicalness trigger switch, when electrode terminal one is subjected to displacement the depression bar trigger switch of Shi Yuqi interlock; Sensor also can be optoelectronic switch, to have an impact triggering optoelectronic switch to light path when electrode terminal one is subjected to displacement; Sensor also can Hall switch etc., triggers when shock by electricity pipe or electrode terminal one are subjected to displacement.
A kind ofly have in the cell electroporation of electric shock pipe above-mentioned, described pilot lamp can be arranged on the casing near electric shock pipe jack, and light illuminates electric shock pipe nearby.Described pilot lamp also can be arranged on permanent seat, described permanent seat can be opened horizontal through hole to install pilot lamp, described clamping cylinder can be made by transparent material, and the pilot lamp on permanent seat can illuminate electric shock pipe from the side through clamping cylinder, forms visual effect more clearly.Further, the transparent clamping cylinder of different size can adapt to the electric shock pipe of different size.
A kind ofly have in the cell electroporation of electric shock pipe above-mentioned, described casing is provided with a display screen, sampling thief is provided with in described casing, described micro-control unit is electrically connected with display screen and power module, described sampling thief to be managed and micro-control unit is electrically connected with electric shock respectively, described samplers sample to electrical signal can pass to micro-control unit and show with the form of wave on a display screen.Power module can produce the electricimpulse that cell electroporation needs, casing is provided with display screen and is used for display instrument and experiment information experimental implementation interface can be shown, micro-control unit in casing can control power module and display screen, micro-control unit comprises programmable micro-chip and other microprocessors etc., sampling thief can gather the electrical signal in cell electroporation process, comprise voltage or current signal, sampling thief comprises the electronic components such as resistance, electrical signal, can with the display of the form of oscillogram on a display screen via micro-control unit process.
A kind ofly to have in the cell electroporation of electric shock pipe above-mentioned, the inner of described cover plate is articulated with on casing, and the outer end of described cover plate is provided with buckle two, described casing is also provided with can with the grab two of described buckle two-phase clamping.Cover plate one end is articulated with on casing, and the other end and the clamping of casing phase, conveniently can cover and open.
Compared with prior art, the present invention has the following advantages:
1, when chock plug is fixed on body end, elastic component produces compressive set, can avoid occurring gap between the second electrode and opening, improve the sealing property between the second electrode and open end-face, thus avoid air to enter liquid sample in cavity, and, when the phenomenon that slightly upwarps appears in chock plug, elastic component also can do certain recovery deformation, ensure that the second electrode still reclines mutually with the end face of edge of opening, thus make the liquid sample in cavity still can not produce air bubble when experimenter's application of sample operates some deviation; In sum, this electric shock pipe can improve the sealing property between the second electrode and opening effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity;
2, extra pressure is transmitted to edge of opening body end via the second electrode is produced when electric shock pipe pushed down by cell electroporation cover plate in the application, strengthen the stopping property of the second electrode and edge of opening, the electrochemistry bubble that in cell electric shock process, solution electrochemistry reaction produces can be compressed to a certain extent, thus reduce its impact on distribution of current in electric shock process, thus improve cell electroporation effect;
3, in the application, cell electroporation can allow experimenter to observe the state of electric shock pipe, contributes to experimenter and observes the carrying out with Control release;
4, in the application, cell electroporation can actual current in showed cell electroporation process or voltage waveform, contributes to experimenter and observes the carrying out with Control release.
Accompanying drawing explanation
Fig. 1 is that the three-dimensional arrangement that in embodiment one, this electric shock is managed when not installing the second electrode is shown
Intention.
Fig. 2 is cuing open of electric shock pipe when chock plug is not fixed in locating structure in embodiment one
View.
Fig. 3 is the partial enlarged drawing in embodiment one.
Fig. 4 is the partial enlarged drawing in embodiment two.
Fig. 5 is the partial enlarged drawing in embodiment three.
Fig. 6 is the sectional view of the electric shock pipe of the second electrode when being fixed in locating structure with chock plug.
Fig. 7 is partial enlarged drawing in embodiment four.
Fig. 8 is partial enlarged drawing in embodiment five.
Fig. 9 is partial enlarged drawing in embodiment six.
Figure 10 is partial enlarged drawing in embodiment seven.
Figure 11 is the structural representation of this cell electroporation in embodiment eight.
Figure 12 is the partial enlarged drawing of Figure 11.
Figure 13 is the explosive view of this cell electroporation in embodiment eight.
Figure 14 is the fundamental diagram of this cell electroporation in embodiment eight.
Figure 15 is the axonometric drawing of this cell electroporation in embodiment eight.
In figure, 1, body; 1a, opening; 1b, connecting cylinder portion; 1c, cylinder chamber; 1d, convex tendon one; 1e, cavity; 1f, annular recesses; 1g, ion conductive layer; 2, the first electrode; 3, the second electrode; 31, the body of rod; 32, cap body; 33, shoulder block portion one; 34, shoulder block portion two; 4, chock plug; 41, through hole one; 42, edge is kept off; 43, convex tendon two; 5, elastic component; 6, stage clip; 7, casing; 8, permanent seat; 81, pedestal; 811, slot; 82, clamping cylinder; 821, jack; 822, lever; 9, cover plate; 10, electrode terminal one; 11, electrode terminal two; 12, inner chamber; 13, through hole two; 14, spring support; 15, spring; 16, buckle two; 17, grab two; 18, keep off along elastic component; 21, electric shock pipe; 24, sampling thief; 25, micro-control unit; 26, display screen; 27, power module; 28, pilot lamp; 29, sensor; 30, flexible connecting member.
Embodiment
Be below specific embodiments of the invention and by reference to the accompanying drawings, technical scheme of the present invention is further described, but the present invention be not limited to these embodiments.
Embodiment one:
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 6, this electric shock pipe comprises body 1, first electrode 2, second electrode 3 and chock plug 4, chock plug 4 is connected by a flexible connecting member 30 with body 1, body 1 inside has the cavity 1e for holding target liq sample, one end of body 1 is provided with the first electrode 2, the other end of body 1 has the opening 1a be connected with cavity 1e, and the working position of the first electrode 2 is connected with cavity 1e, and the edge of opening 1a has the end face of annular, second electrode 3 is arranged in the chock plug 4 and outer end of the second electrode 3 and can be electrically connected by the opening of chock plug 4 and outside, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, elastic component is provided with between chock plug 4 and the second electrode 3, the outer side of elastic component leans with chock plug 4 or is connected, medial surface and second electrode 3 of elastic component lean, the periphery of opening 1a has can be fixed on body 1 end by chock plug 4 and make elastic component produce the locating structure of compressive set, locating structure comprises the connecting cylinder portion 1b be connected as a single entity with the end of body 1, connecting cylinder portion 1b offers the cylinder chamber 1c for chock plug 4 grafting, the chamber wall of cylinder chamber 1c has convex tendon one 1d, the outer side of chock plug 4 has can with the convex tendon 2 43 of convex tendon one 1d phase clamping, in the present embodiment, convex tendon one 1d and convex tendon 2 43 are complete annulars, can also be other shapes such as some sections of arc compositions.
When using this electric shock pipe, the liquid sample of the material comprising cell and cell will be injected is filled in forming a liquid convex surface to cavity 1e, then make chock plug 4 be fixed on body 1 end by fixed sturcture and make the elastic component between chock plug 4 and the second electrode 3 produce compressive set, now the second electrode 3 is crushed on opening 1a end face, first electrode 2 and the second electrode 3 all with liquid communication in cavity 1e, the first electrode 2 is made to be connected with the pulse power with the second electrode 3 again, electric discharge makes to form electric field in electric shock pipe 21, cytolemma is made to have certain permeability, thus the target substance in liquid sample is entered in cell.When chock plug 4 is fixed on body 1 end, elastic component produces compressive set, can avoid occurring gap between the second electrode 3 and opening 1a, improve the sealing property between the second electrode 3 and opening 1a end face, thus avoid air to enter liquid sample in cavity 1e, and, when the phenomenon that slightly upwarps appears in chock plug 4, elastic component also can do certain recovery deformation, ensure that the second electrode 3 still reclines mutually with the end face at opening 1a edge, thus make the liquid sample in cavity 1e still can not produce air bubble when experimenter's application of sample operates some deviation; Effectively improve the sealing property between the second electrode 3 and opening 1a, thus avoid the air in the external world when adding liquid sample to enter in cavity 1e.
Body 1 in the present embodiment, chock plug 4 adopt insulating material to make, first electrode 2 and the second electrode 3 adopt electro-conductive material to make, first electrode 2 both can directly be fixed in body 1, also equally with the second electrode 3 can be arranged on inside a chock plug 4 and seal body 1 cavity 1e.Elastic component is an elastic component 5 adopting rubber-plastics material to make, chock plug 4 comprises the tubular portion with through hole 1, second electrode 3 is plugged in through hole 1, the cap body 32 that second electrode 3 comprises the body of rod 31 and is connected with the body of rod 31 one end, the body of rod 31 the other end can electrically connect through chock plug 4 and outside, elastic component 5 is socketed on the body of rod 31 outer side, the outer end of chock plug 4 has along the radially inner gear of through hole 1 along 42, the outer face of elastic component 5 is resisted against gear along on 42, and the inner face of this elastic component 5 is resisted against on cap body 32.Rubber-plastics material comprises plastics, rubber, silica gel etc., by chock plug 4 regularly body 1 end time, second electrode 3 reclines mutually with the end face at the cavity 1e opening 1a edge of body 1, elastic component 5 produces compression set, improve the stopping property of the second electrode 3 and opening 1a edge end face, and along 42, the body of rod 31 can extend to that the outside of chock plug 4 is convenient to be energized to the second electrode 3 through gear; Certain second electrode 3 also can not extend to the outside of chock plug 4, and the electric connection contacts of chock plug 4 outside realizes being electrically connected with the ends contact of the second electrode 3.Elastic component 5 generally can make a complete round, may not be complete round, as long as flexible and can grafting second electrode 3.
The present embodiment Elastic part 5 is independently elastic components 5, is arranged in an interference fit in through hole 1, or is arranged in through hole 1 in the mode such as bonding; Be provided with shoulder block portion 1 between elastic component 5 and cap body 32, shoulder block portion 1 can make round and elastic component 5 and cap body 32 and coaxially arrange, and the size in shoulder block portion 1 or diameter are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.Shoulder block portion 1 also can be incomplete round or other shapes, and its size or spacial yardstick also need to be greater than the diameter of the body of rod 31 and be less than the diameter of cap body 32.Shoulder block portion 1 separates cap body 32 and elastic component 5 and makes cap body 32 oppress elastic component 5 to need to carry out pressure conduction by shoulder block portion 1, and the diameter in shoulder block portion 1 or size are less than cap body 32 and elastic component 5, produce thus and impel elastic component 5 more easily deformational displacement to occur compared with the larger pressure on small area of contact.When chock plug 4 is fixed in locating structure, second electrode 3 reclines mutually with opening 1a end face, elastic component 5 is subject to the pressure from the second electrode 3 and is in compressed state, produce pressure conduction by this compressive set and increase sealing property to the opening 1a end face of body 1 end, and chock plug 4 have upwarp on a small quantity in produce certain recovery deformation and keep the second electrode 3 to continue to be pressed on the opening 1a end face of body 1 end.
Shoulder block portion 1 is independently parts, and shoulder block portion 1 is enclosed within the body of rod 31 between elastic component 5 and cap body 32, its material can insulate or nonisulated can; The outer side of elastic component 5 and the inwall of through hole 1 fit and form sealing, and the medial surface of this elastic component 5 and the outer side of the body of rod 31 fit and form sealing.
Convex tendon one 1d and convex tendon 2 43 can be complete annular or discontinuous annular, can reach the effect of clamping.The protrusion of convex tendon one 1d and convex tendon 2 43 can not be clearly, and the size adopting chock plug 4 to fill in body 1 part is a bit larger tham the size inside connecting cylinder portion 1b, reaches the object of location in an interference fit; Chock plug 4 and the clamping of connecting cylinder portion 1b phase, make both connect be separated all very convenient, improve fluid injection and move the convenience of liquid after completing electric shock, certainly also can adopt the mode be threaded, when adopting screw thread, chock plug 4 rotation can make it be fixed on connecting cylinder portion 1b.As another kind of situation, locating structure can be arranged at grab on chock plug 4 one and to be arranged on body 1 can with the buckle one of grab one phase clamping.
As shown in Figure 2, the end face at this enforcement middle tube body 1 opening 1a edge offers annular recesses 1f, fill in liquid sample in cavity 1e and form a convex surface on opening 1a, convex surface surplus liquid is also extruded when pressing down sealed open 1a and flows into annular recesses 1f by the second electrode 3 contact liq convex surface, does not affect cell electroporation process in cavity 1e liquid sample.
As shown in Figure 2, ion conductive layer 1g is provided with in the cavity 1e of body 1, the subsurface of ion conductive layer 1g contacts with the first electrode 2, the upper aspect of ion conductive layer 1g can with target liq sample contact, ion conductive layer 1g separates cell sample and contacts with the first the direct of electrode 2, thus avoiding cell sample to be subject to the direct infringement of electrochemical reaction near the first electrode 2, ion conductive layer 1g contains the source of soluble salt composition as ion, infiltrates salts solution and forms conductive ion.
Embodiment two:
As shown in Figure 4, the present embodiment is roughly the same with embodiment one, difference is, shoulder block portion 1 and cap body 32, the body of rod 31 are one-body molded, the inner face of elastic component 5 is resisted against on the end face in shoulder block portion 1, when in the body of rod 31 grafting and elastic component 5 time, cap body 32 and elastic component 5 are separated by this shoulder block portion 1 and by its conducting pressure.The shape in shoulder block portion 1 can be annular, discontinuous annular, Polygons and other shapes, and its size or diameter are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.
Embodiment three:
As shown in Figure 5, the present embodiment is roughly the same with embodiment one or embodiment two, and difference is, shoulder block portion 1 be installed on elastic component 5 or shoulder block portion 1 one-body molded with elastic component 5, shoulder block portion 1 and cap body 32 lean.The shape in shoulder block portion 1 can be annular, discontinuous annular, Polygons and other shapes, and its size or diameter are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.
Embodiment four:
As shown in Figure 7, the present embodiment is roughly the same with embodiment one, difference is, as another kind of elastic component scheme in the present embodiment, elastic component be through hole 1 inwall extends internally out along through hole 1 there is elastic gear along elastic component 18, keep off be resisted against the body of rod 31 along elastic component 18 outer side on and form sealing, gear is also provided with shoulder block portion 2 34 along between elastic component 18 and cap body 32, shoulder block portion 2 34 is independent to be arranged, shoulder block portion 2 34 can make round and coaxially arrange along elastic component 18 and cap body 32 with gear, the diameter in shoulder block portion 2 34 is greater than the diameter of the body of rod 31 and is less than the diameter of cap body 32.Shoulder block portion 2 34 also can be incomplete round or other shapes, and its size or spacial yardstick are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.This gear is identical with chock plug 4 along the material of elastic component 18, and the inwall of outside and chock plug 4 main body is connected as a single entity and intermediate formation opening, and the body of rod 31 of the second electrode 3 is plugged on the opening part of gear along elastic component 18 with shrink-fit or the mode such as bonding.The main body of electric shock pipe 21, for the needs of tolerance range, generally needs to make have certain intensity to prevent distortion; And the mode reducing this partial intensities can be adopted with the gear that chock plug 4 is connected as a single entity to realize elasticity as elastic component along elastic component 18, such as this partial design become thickness to compare other parts thinner thus more easily pressure deformation can occur.This gear generally can make a complete round along elastic component 18, may not be complete round, if the elasticity of maintaining and can grafting second electrode 3 just passable.
Embodiment five:
As shown in Figure 8, the present embodiment is roughly the same with embodiment four, and difference is, shoulder block portion 2 34 and cap body 32 in the present embodiment, the body of rod 31 are all one-body molded, and the inner face kept off along elastic component 18 is resisted against on the end face in shoulder block portion 2 34.The shape in shoulder block portion 2 34 can be annular, discontinuous annular, Polygons and other shapes, and its size or diameter are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.
Embodiment six:
As shown in Figure 9, the present embodiment is roughly the same with embodiment four or five, and difference is, in the present embodiment, shoulder block portion 2 34 is one-body molded along elastic component 18 with gear, and shoulder block portion 2 34 and cap body 32 lean.The shape in shoulder block portion 2 34 can be annular, discontinuous annular, Polygons and other shapes, and its size or diameter are greater than the diameter of the body of rod 31 and are less than the diameter of cap body 32.
Embodiment seven:
As shown in Figure 10, the present embodiment is roughly the same with embodiment one, difference is, as the scheme of the third elastic component, the present embodiment Elastic part is a stage clip 6, chock plug 4 comprises the tubular portion with through hole 1, second electrode 3 is arranged in through hole 1, the cap body 32 that second electrode 3 comprises the body of rod 31 and is connected with the body of rod 31 one end, the body of rod 31 the other end can be electrically connected by the opening of chock plug 4 and outside, stage clip 6 is socketed on the body of rod 31 outer side, the outer end of chock plug 4 has along the radially inner gear of through hole 1 along 42, the outer face of stage clip 6 is resisted against gear along on 42, the inner face of this stage clip 6 is resisted against on cap body 32.When chock plug 4 is fixed on body 1 end, stage clip 6 can produce compression set, the sealing property between body 1 end of improving the second electrode 3 and opening 1a edge.
Embodiment eight:
As shown in Figure 11 to 15, provide a kind of cell electroporation with electric shock pipe 21 in the present embodiment, this cell electroporation comprises casing 7, is provided with permanent seat 8 in casing 7, permanent seat 8 offers jack 821; Cell electroporation also comprises the electric shock pipe 21 be plugged in this jack 821, electric shock pipe 21 comprises body 1, first electrode 2, second electrode 3 and chock plug 4, body 1 inside has the cavity 1e for holding target liq sample, one end of body 1 is provided with the first electrode 2, the other end of body 1 has the opening 1a be connected with cavity 1e, the working position of the first electrode 2 is connected with cavity 1e, and the edge of opening 1a has the end face of annular; Second electrode 3 is arranged in chock plug 4 and the outer end of the second electrode 3 can by the opening of chock plug 4 and external electrical connections, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, elastic component is provided with between chock plug 4 and the second electrode 3, the outer side of elastic component leans with chock plug 4 or is connected, medial surface and second electrode 3 of elastic component lean, and the periphery of opening 1a has can be fixed on body 1 end by chock plug 4 and make elastic component produce the locating structure of compressive set; The inner of jack 821 has the electrode terminal 1 that can be electrically connected with the first electrode 2, casing 7 is provided with the cover plate 9 for hiding jack 821 outer end, cover plate 9 is provided with the electrode terminal 2 11 that can be electrically connected with the second electrode 3, casing 7 is also provided with the power module 27 be electrically connected with electrode terminal 1 and electrode terminal 2 11.
This cell electroporation in use, liquid sample can be filled in the cavity 1e of body 1, then chock plug 4 is covered, then close after electric shock pipe 21 is placed in jack 821 cover plate 9, electrode terminal 1 is electrically connected with the first electrode 2, and electrode terminal 2 11 is electrically connected with the second electrode 3, then starts power pulse electric discharge, make to form electric field in electric shock pipe 21, and then extracellular material is injected in cell.Owing to being also provided with the elastic component for strengthening the reactive force between the second electrode 3 and opening 1a end face in chock plug 4, therefore can avoid occurring gap between the second electrode 3 and opening 1a; Further, when the phenomenon that slightly upwarps appears in chock plug 4, elastic component also can do certain recovery deformation, ensures that the second electrode 3 still reclines mutually with body 1 end of opening 1a end face, thus avoids extraneous air to enter cavity 1e; Also make electric shock pipe 21 bubble that oneself electrochemical reaction inner produces among electric shock process suffer restraints simultaneously.To sum up, electric shock pipe 21 in this cell electroporation can improve the sealing property between the second electrode 3 and opening 1a end face effectively, thus avoid entering in electric shock pipe 21 cavity 1e at the air extraneous in set-up procedure that shocks by electricity forming aeration electricity turn, and reduce the bubble of electrochemical reaction generation in electric shock process to the impact of electric current, thus improve the electroporation performance of this cell electroporation.
As shown in Figure 11, Figure 12 and Figure 13, permanent seat 8 comprises pedestal 81 and clamping cylinder 82, the jack 821 offered in clamping cylinder 82, a slot 811 is offered in pedestal 81, clamping cylinder 82 to be inserted in slot 811 and both detachably interconnectings, the bottom of clamping cylinder 82 reclines mutually with the bottom land of slot 811, and the top of clamping cylinder 82 is provided with at least one lever 822.
As shown in figure 12, an inner chamber 12 is also offered in pedestal 81, the bottom land of slot 811 offers through hole 2 13, the two ends of through hole 2 13 are connected with inner chamber 12 with jack 821 respectively, spring support 14 and spring 15 is provided with in inner chamber 12, the inner of spring 15 is fixed on spring support 14, and the outer end of spring 15 is connected with the inner of electrode terminal 1, and the outer end of electrode terminal 1 can be passed through hole 2 13 and be inserted in jack 821.After being designed with spring support 14 and spring 15, when cover plate 9 covers jack 821 opening 1a, first electrode 2 and the second electrode 3 can be electrically connected with electrode terminal 1 and electrode terminal 2 11 respectively, spring 15 when shocking by electricity pipe 21 pushed down by cover plate 9, and by the compression larger extra pressure of generation, this pressure is transmitted to cavity 1e opening 1a via the second electrode 3, strengthens the stopping property of the second electrode 3 and cavity 1e opening 1a.Due to electrochemical reaction electrolysis meeting creating pockets of air in cell electric shock process, when between the second electrode 3 and cavity 1e opening 1a, pressure ratio is larger, these electrochemistry bubbles can be compressed and reduce its impact on distribution of current in electric shock process, thus improve cell electroporation effect.Cell has shocked by electricity back shroud 9 when opening, and electric shock pipe 21 can eject a part under the effect of spring 15 from jack 821, convenient pickup.
The inner of cover plate 9 is articulated with on casing 7, the outer end of cover plate 9 is provided with buckle 2 16, casing 7 is also provided with can with the grab 2 17 of buckle 2 16 phase clamping, the material that cover plate 9 clamping cylinder 82 is greater than 50% by transmittance is made, the sensor 29 for detecting electric shock pipe 21 change in displacement is provided with in pedestal 81 annex, micro-control unit 25 is provided with in casing 7, micro-control unit 25 is electrically connected with sensor 29, also be provided with the pilot lamp 28 be electrically connected with micro-control unit 25 in pedestal 81, pilot lamp 28 is also electrically connected with power module 27.Pilot lamp 28 is except having the effect of instruction electric shock pipe 21 connection state, also illuminating effect can be had simultaneously, the light source of higher brightness such as LED etc. can be adopted to illuminate electric shock pipe 21, sensor 29 can be mechanicalness trigger switch, when electrode terminal 1 is subjected to displacement the depression bar trigger switch of Shi Yuqi interlock; Sensor 29 also can be optoelectronic switch, to have an impact triggering optoelectronic switch to light path when electrode terminal 1 is subjected to displacement; Sensor 29 also can Hall switch etc., triggers when shock by electricity pipe 21 or electrode terminal 1 are subjected to displacement.
Pilot lamp 28 is arranged on the casing 7 near electric shock pipe 21 jack 821, and light illuminates electric shock pipe 21 nearby.As other situations, pilot lamp 28 also can be arranged on permanent seat 8, permanent seat 8 can leave horizontal through hole to install pilot lamp 28.Clamping cylinder 82 can be made by transparent material, and the pilot lamp 28 on permanent seat 8 can illuminate electric shock pipe 21 from the side through clamping cylinder 82, forms visual effect more clearly.Casing 7 is provided with a display screen 26, is provided with sampling thief 24 in casing 7, micro-control unit 25 is electrically connected with display screen 26 and power module 27, and sampling thief 24 is electrically connected with electric shock pipe 21 and micro-control unit 25 respectively.Power module 27 can produce the electricimpulse that cell electroporation needs, display screen 26 is used for display instrument and experiment information can show experimental implementation interface, micro-control unit 25 can control power module 27 and display screen 26, micro-control unit 25 comprises programmable micro-chip and other microprocessors etc., sampling thief 24 can gather the electrical signal in cell electroporation process, comprise voltage or current signal, sampling thief 24 comprises the electronic components such as resistance, electrical signal processes via micro-control unit 25, correlation parameter can be shown on the display 26 with the form of numerical value or oscillogram.
Shoulder block portion 1 in above embodiment and shoulder block portion 2 34 can be all complete ring-types, and also can use some sections of arc compositions, the simple transformation of its structural shape is all identical with the technical program essence.
Specific embodiment described in literary composition is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.

Claims (25)

1. an electric shock pipe, described electric shock pipe comprises body (1), first electrode (2), second electrode (3) and chock plug (4), described body (1) inside has the cavity (1e) for holding target liq sample, it is characterized in that, one end of described body (1) is provided with described first electrode (2), the other end of described body (1) has the opening (1a) be connected with described cavity (1e), the working position of described first electrode (2) is connected with described cavity (1e), the edge of described opening (1a) has the end face of annular, described second electrode (3) is arranged in chock plug (4) and the outer end of described second electrode (3) and can be electrically connected by the opening of described chock plug (4) and outside, the inner face of described second electrode (3) can fit with the annular end face at opening (1a) edge, the elastic component (5) be connected with the second electrode (3) is provided with in described chock plug (4), the outside of described elastic component (5) is connected with described chock plug (4), the inner side of described elastic component is connected with described second electrode (3), the periphery of described opening (1a) has can be fixed on body (1) end by chock plug (4) and make described elastic component produce the locating structure of compressive set.
2. electric shock pipe according to claim 1, it is characterized in that, described chock plug (4) comprises the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) comprises the body of rod (31) and cap body (32), the described body of rod (31) one end and described cap body (32) are connected, the described body of rod (31) the other end can be electrically connected by the opening of described chock plug (4) and outside, described elastic component (5) is socketed on the body of rod (31) outer side, the outer end of described chock plug (4) has along the radially inner gear of through hole one (41) along (42), the outer face of described elastic component (5) is resisted against gear on (42), the inner face of this elastic component (5) is resisted against on cap body (32).
3. electric shock pipe according to claim 2, it is characterized in that, be provided with shoulder block portion one (33) between described elastic component (5) and described cap body (32), the size in described shoulder block portion one (33) is greater than the diameter of the body of rod (31) and is less than the diameter of cap body (32).
4. electric shock pipe according to claim 3, it is characterized in that, described shoulder block portion one (33) is installed in described cap body (32) and is above connected with described cap body (32), the body of rod (31), and the inner face of described elastic component (5) is resisted against on the end face in described shoulder block portion one (33).
5. electric shock pipe according to claim 3, it is characterized in that, described shoulder block portion one (33) is installed in described elastic component (5) and is above connected with described elastic component (5), and described shoulder block portion one (33) leans with described cap body (32).
6. electric shock pipe according to claim 1, it is characterized in that, described chock plug (4) comprises the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) comprises the body of rod (31) and cap body (32), the described body of rod (31) one end and described cap body (32) are connected, the described body of rod (31) the other end can be electrically connected by the opening of described chock plug (4) and outside, described elastic component (5) has elastic gear along elastic component (18) for what described through hole one (41) inwall extended internally out along through hole one (41), described gear is resisted against on the outer side of the described body of rod (31) along elastic component (18).
7. electric shock pipe according to claim 6, it is characterized in that, described gear is provided with shoulder block portion two (34) along between elastic component (18) and described cap body (32), and the size in described shoulder block portion two (34) is greater than the diameter of the body of rod (31) and is less than the diameter of cap body (32).
8. electric shock pipe according to claim 7, it is characterized in that, described shoulder block portion two (34) is installed in described cap body (32) and is above connected with described cap body (32), the body of rod (31), and described gear is resisted against on the end face in described shoulder block portion two (34) along the inner face of elastic component (18).
9. electric shock pipe according to claim 7, it is characterized in that, described shoulder block portion two (34) is installed in described gear and is connected along elastic component (18) with described gear along elastic component (18) is upper, and described shoulder block portion two (34) leans with described cap body (32).
10. electric shock pipe according to claim 1, it is characterized in that, described elastic component (5) is a stage clip (6), described chock plug (4) comprises the tubular portion with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) comprises the body of rod (31) and cap body (32), the described body of rod (31) one end and described cap body (32) are connected, the described body of rod (31) the other end can be electrically connected by the opening of described chock plug (4) and outside, described stage clip (6) is socketed on the body of rod (31) outer side, the outer end of described chock plug (4) has along the radially inner gear of through hole one (41) along (42), the outer face of described stage clip (6) is resisted against gear on (42), the inner face of this stage clip (6) is resisted against on cap body (32).
11. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, described locating structure comprises the connecting cylinder portion (1b) be connected as a single entity with the end of described body (1), described connecting cylinder portion (1b) offers the cylinder chamber (1c) for described chock plug (4) grafting, the chamber wall of described cylinder chamber (1c) has convex tendon one (1d), the outer side of described chock plug (4) has can with the convex tendon two (43) of described convex tendon one (1d) phase clamping.
12. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, described locating structure comprises the grab one be arranged on described chock plug (4), described body (1) has can with the buckle one of described grab one phase clamping.
13. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, is characterized in that, the end face at described opening (1a) edge offers annular recesses (1f).
14. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, described chock plug (4) is connected by a flexible connecting member (30) with described body (1).
15. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, outside and the described chock plug (4) of described elastic component (5) fit and form sealing, and the inner side of described elastic component (5) and the outer side of described second electrode (3) fit and formed and seal.
16. electric shock pipes according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10, it is characterized in that, ion conductive layer (1g) is provided with in the cavity (1e) of described body (1), the subsurface of described ion conductive layer (1g) contacts with the first electrode (2), the upper aspect of described ion conductive layer (1g) can with target liq sample contact.
17. 1 kinds have electric shock pipe cell electroporation, described cell electroporation comprises casing (7), be provided with permanent seat (8) in described casing (7), described permanent seat (8) offer jack (821), it is characterized in that, described cell electroporation also comprises the electric shock pipe (21) be plugged in this jack (821), described electric shock pipe (21) comprises body (1), first electrode (2), second electrode (3) and chock plug (4), described body (1) inside has the cavity (1e) for holding target liq sample, one end of described body (1) is provided with described first electrode (2), the other end of described body (1) has the opening (1a) be connected with described cavity (1e), the working position of described first electrode (2) is connected with described cavity (1e), the edge of described opening (1a) has the end face of annular, described second electrode (3) is arranged in chock plug (4) and the outer end of described second electrode (3) can by the opening of described chock plug (4) and external electrical connections, the inner face of described second electrode (3) can fit with the annular end face at opening (1a) edge, the elastic component (5) be connected with the second electrode (3) is provided with in described chock plug (4), the outside of described elastic component (5) is connected with described chock plug (4), the inner side of described elastic component is connected with described second electrode (3), the periphery of described opening (1a) has can be fixed on body (1) end by chock plug (4) and make described elastic component produce the locating structure of compressive set, the inner of described jack (821) has the electrode terminal one (10) that can be electrically connected with described first electrode (2), described casing (7) is provided with the cover plate (9) for hiding jack (821) outer end, described cover plate (9) is provided with the electrode terminal two (11) that can be electrically connected with described second electrode (3), described casing (7) is also provided with the power module (27) be electrically connected with described electrode terminal one (10) and electrode terminal two (11).
The 18. cell electroporations with electric shock pipe according to claim 17, it is characterized in that, described permanent seat (8) comprises pedestal (81) and clamping cylinder (82), described jack (821) is offered in described clamping cylinder (82), offer a slot (811) in described pedestal (81), described clamping cylinder (82) to be inserted in slot (811) and both detachably interconnectings.
The 19. cell electroporations with electric shock pipe according to claim 18, it is characterized in that, the material that described clamping cylinder (82) is greater than 50% by transmittance is made.
The 20. cell electroporations with electric shock pipe according to claim 18, it is characterized in that, the bottom of described clamping cylinder (82) reclines mutually with the bottom land of described slot (811), and the top of described clamping cylinder (82) is provided with at least one lever (822).
The 21. cell electroporations with electric shock pipe according to claim 18, it is characterized in that, an inner chamber (12) is also offered in described pedestal (81), the bottom land of described slot (811) offers through hole two (13), the two ends of described through hole two (13) are connected with inner chamber (12) with described jack (821) respectively, spring support (14) and spring (15) is provided with in described inner chamber (12), the inner of described spring (15) is fixed on spring support (14), the outer end of described spring (15) is connected with the inner of described electrode terminal one (10), the outer end of described electrode terminal one (10) can be passed described through hole two (13) and be inserted in described jack (821).
22. according to claim 17 or 18 or 19 or 20 or 21 having electric shock pipe cell electroporation, it is characterized in that, the material that described cover plate (9) is greater than 50% by transmittance is made, the sensor (29) for detecting electric shock pipe (21) change in displacement is provided with in described pedestal (81), the micro-control unit (25) be connected with sensor (29) is provided with in described casing (7), pilot lamp (28) is also provided with in described casing (7), described pilot lamp (28) is connected with described power module (27), the signal of described sensor (29) can pass to described micro-control unit (25) and be used for controlling pilot lamp (28).
23. according to claim 17 or 18 or 19 or 20 or 21 having electric shock pipe cell electroporation, it is characterized in that, described casing (7) is provided with a display screen (26), sampling thief (24) is provided with in described casing (7), described micro-control unit (25) is connected with display screen (26) and power module (27), and described sampling thief (24) manages (21) with electric shock respectively and micro-control unit (25) is connected.
The 24. cell electroporations with electric shock pipe according to claim 23, it is characterized in that, described samplers sample to electrical signal can pass to micro-control unit (25) and show with the form of wave on display screen (26).
25. according to claim 17 or 18 or 19 or 20 or 21 having electric shock pipe cell electroporation, it is characterized in that, the inner of described cover plate (9) is articulated with on casing (7), the outer end of described cover plate (9) is provided with buckle two (16), described casing (7) is also provided with can with the grab two (17) of described buckle two (16) phase clamping.
CN201410722470.9A 2014-12-02 2014-12-02 A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe Active CN104403943B (en)

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CN201410722470.9A CN104403943B (en) 2014-12-02 2014-12-02 A kind of cell electroporation shocking by electricity pipe and there is electric shock pipe
JP2017530204A JP6629858B2 (en) 2014-12-02 2015-12-02 Electric shock pipe and electroporation apparatus having the electric shock pipe
EP15864958.2A EP3228695A4 (en) 2014-12-02 2015-12-02 An electric shock pipe and cell electroporator having electric shock pipe
PCT/IB2015/059297 WO2016088062A1 (en) 2014-12-02 2015-12-02 An electric shock pipe and cell electroporator having electric shock pipe
US15/532,182 US10472601B2 (en) 2014-12-02 2015-12-02 Shock tube and cell electroporation device with the shock tube
US16/586,989 US11965149B2 (en) 2014-12-02 2019-09-29 Shock tube and cell electroporation device with the shock tube
JP2019219959A JP6838125B2 (en) 2014-12-02 2019-12-04 An electric shock pipe and an electroporation device having the electric shock pipe

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US10472601B2 (en) 2014-12-02 2019-11-12 Jian Chen Shock tube and cell electroporation device with the shock tube
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CN110437999A (en) * 2019-07-15 2019-11-12 广州蛟龙细胞医药科技有限公司 A kind of electroporation
CN114196533A (en) * 2021-11-01 2022-03-18 上海盟德生物科技有限公司 Microchannel cell electrotransformation instrument

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