CN205205151U - Electric shock pipe - Google Patents
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- CN205205151U CN205205151U CN201520981477.2U CN201520981477U CN205205151U CN 205205151 U CN205205151 U CN 205205151U CN 201520981477 U CN201520981477 U CN 201520981477U CN 205205151 U CN205205151 U CN 205205151U
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Abstract
The utility model provides an electric shock pipe belongs to cell fusion equipment technical field. It has been solved current electric shock pipe and has produced technical problem such as high -tension arc easily. This electric shock pipe includes chock plug and integrated into one piece's body, and the body is inside to have the cavity that is used for holding target liquid sample, and the opening and outside electric connection of chock plug can be passed through in the outer end that is equipped with second electrode and second electrode in the chock plug, and the middle part in the body is equipped with the first electrode that is linked together with the cavity, and first electrode and body form and seal, and the tube part who is located the first electrode downside is for preventing first electrode and the second electrode extension section at body outside production high -tension arc. The utility model discloses in make the air disruptive distance between first electrode and second electrode extra long greatly through setting up extension section, even applyed very high high voltage, can prevent effectively also that the punch through from appearing in first electrode and second electrode in the air outside the body.
Description
Technical field
The utility model belongs to biological medicine plant and instrument technical field, relates to the electric shock pipe in a kind of cell electroporation equipment, particularly electroporation.
Background technology
Electroporation be utilize electricimpulse to introduce in cell cannot the macromolecular technology of permeates cell membranes, it is a kind of extensive employing and obtains the method for test cell line and gene therapy of strong preference.When applying highfield, cytolemma temporarily becomes porous character and can through foreign material, such as macromole.Cytolemma electricity penetration depends on the various parameters of electric field, such as pulse pattern, pulsed voltage, pulse duration, number of pulses and other experiment conditions; Equipment at present for cell electroporation mainly contains cell electroporation, pole cup etc.
China patent CN1965079B discloses a kind of electroporation device with long hollow member, this electroporation device comprises long hollow member, to provide uniform electric field in electroporation process, wherein particularly, perform electroporation be by be full of in hollow member comprise cell and will inject cell the liquid sample of material after, apply electricimpulses by pair of electrodes from long hollow member two ends.
Applicant once declared one section of " method and apparatus of electroporation " (METHODSANDDEVICESFORELECTROPORATION by name; US2013/0052711A1) United States Patent (USP), a kind of sampling receptacle is described in this patent, here claim with the pipe belt that shocks by electricity, electric shock manages the sampling receptacle be namely equivalent in aforesaid U.S. Patent application, its effect adds containing cell and the liquid sample of material that will inject cell in pipe, at the upper/lower terminal of electric shock pipe, top electrode and lower electrode are set respectively, be connected with lower electrode with top electrode by cell electroporation, thus make to form electric field in electric shock pipe, and then extracellular material is injected in cell.After filling liquid sample in electric shock pipe, sample shape is fixed, and can not be formed bending between electrode by fluid surface as traditional open pole cup, eliminate the liquid internal electric field after liquid curved surface more even, can improve electroporation efficiency.And liquid sample being injected after in electric shock pipe, before starting electric shock, need in electric shock pipe to have prevented air residual from forming aeration distribution of current.In that patent, applicant is designed with an annular recesses be connected with electric shock tube chamber on the tube wall of electric shock pipe, experimenter is sustainable to injection liquid sample body in electric shock tube chamber until after fluid surface protrusion electric shock tube chamber, to contact until be pressed onto the upper edge of the tube chamber that shocks by electricity under the polar cap that power on liquid convex surface again, form the sealing to liquid inside cavity, and the liquid overflowed on a small quantity flows in annular recesses.This design generally can prevent having residual air to affect experiment in electric shock pipe.
But, applicant finds in constantly putting into practice, the experiment needs having some experiments particularly to relate to microorganism apply several kilovolts even voltage of more than ten thousand volts at electric shock pipe two ends, and sample usually needs to cool and causes electric shock pipe outer wall easily to produce water of condensation making it to be in very moistening state, the pipe outside air that shocks by electricity under high-voltage and moistening environment is likely broken down by high-voltage and affects the carrying out of electroporation experiment.
Summary of the invention
The utility model is the problems referred to above existed for existing technology, provides a kind of electric shock pipe.Technical problem to be solved in the utility model is: how to reduce in the air between electric shock pipe two electrodes outside electric shock pipe and produce high-voltage arc phenomenon.
The purpose of this utility model realizes by following technical proposal:
A kind of electric shock pipe, described electric shock pipe comprises chock plug and integrated body, described tubular body has the cavity for holding target liq sample, one end of described body has the opening be connected with described cavity, the second electrode is provided with and the outer end of described second electrode can be electrically connected by the opening of described chock plug and outside in described chock plug, the inner face of described second electrode can fit with the annular end face of edge of opening, it is characterized in that, middle part in described body is provided with the first electrode be connected with cavity, described first electrode and body are formed and seal, the described body portion be positioned on the downside of the first electrode is the extension that can prevent the first electrode and the second electrode from producing high-voltage arc outside described body.
Its principle of work is as follows: this electric shock pipe in use, in cavity, the liquid sample of the material comprising cell and cell will be injected is filled in from the opening of body, then chock plug is fixed on body end and the annular end face at the inner face of the second electrode and body open edge is fitted, first electrode and the second electrode all with liquid communication in cavity, be electrically connected with the first electrode in the extension that electrode terminal can extend into body, the outer end of the second electrode can be electrically connected by the opening of chock plug and outside, first electrode is connected with the pulse power with the second electrode, electric discharge makes to form electric field in the cavity of electric shock pipe, cytolemma is made to have certain permeability, thus the target substance in liquid sample is entered in cell.In the technical program, the body portion be positioned on the downside of the first electrode is the extension that can prevent the first electrode and the second electrode from producing high-voltage arc outside body, the good insulation preformance of extension, generally can not be breakdown under pulsed voltage, first electrode and the second electrode to produce high-voltage arc outside body, extension can only be walked around, by arranging extension, the first electrode and the second interelectrode air breakdown distance are lengthened greatly, even if be applied with very high high-voltage, also can effectively prevent from occurring punch-through in the first electrode and the air of the second electrode outside body.Thus ensure that electric current realizes electroporation to the target liq sample in cavity.
In addition, this extension also has the effect as handle, facilitates user's taking and placing to shock by electricity and manages.
Body in the technical program, chock plug all adopt insulating material to make, and the first electrode and the second electrode adopt electro-conductive material to make, and this part is prior art, and not in this to go forth for the material of embody rule.In addition, the first electrode both can directly be fixed in body, also equally with the second electrode can be arranged on inside a chock plug and seal body cavity.
In above-mentioned electric shock pipe, the length of described extension is general between 1mm to 40mm as required, and can be between 2mm to 30mm as preferred extension length, that more conventional be 5mm to 20mm.When the length such as when extension is greater than the length of cavity, the path distance that first electrode and the second electrode reach air breakdown outside body is at least the length of the cavity of three times, and such first electrode and the second electrode may produce high-voltage arc hardly outside body.
In above-mentioned electric shock pipe, described body adopts the thickness of pipe that the made of plastic and thickness of pipe of extension is less than described cavity place.The thickness of pipe at cavity place is thicker, prevents the high-voltage breakdown between the first electrode and the second electrode.The thickness of pipe that simultaneously thickness of pipe of extension is less than cavity place is convenient to the first electrode and is extend in body from extension and be arranged on the middle part of body, is also convenient to be electrically connected with the first electrode in extension that electrode terminal extend into body.
In above-mentioned electric shock pipe, described body is greater than described body at the pore diameter at described cavity place at the pore diameter at extension place and forms step in body, the second described electrode has convex shoulder and is positioned at the Access Division on the upside of convex shoulder, the end face of described convex shoulder contacts with above-mentioned step, and described Access Division is connected in the pore at described cavity place.Step and convex shoulder cooperatively interact and can play the effect of positioning limit, improve while installing convenience and ensure stopping property, prevent target liq sample from leaking.
In above-mentioned electric shock pipe, be provided with contact part on the downside of the convex shoulder of described second electrode, the diameter of described contact part is less than the diameter of convex shoulder and the tube wall of contact part and described body extension exists gap.Also be convenient to place the clamping of the second electrode and install by contact part.
In above-mentioned electric shock pipe, the length of described contact segment is general shorter, prevents the lower end of the first electrode distance extension too near and walks around extension generation electric arc.
In above-mentioned electric shock pipe, be provided with the elastic component be connected with the second electrode in described chock plug, the outside of described elastic component is connected with described chock plug, and the inner side of described elastic component is connected with described second electrode.When chock plug is fixed on body end, elastic component produces compressive set, elastic component and chock plug and the second electrode are formed seal, can avoid occurring gap between the second electrode and the opening of body, air is avoided to enter liquid sample in cavity, and, when slightly upwarping appears in chock plug time, elastic component also can do certain recovery deformation, ensure that the second electrode still reclines mutually with the end face of edge of opening, thus make the liquid sample in cavity still can not produce air bubble when experimenter's application of sample operates some deviation; In sum, the technical program improves the sealing property between the second electrode and opening effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity.
In above-mentioned electric shock pipe, described chock plug comprises the tubular portion with through hole one, described second electrode is arranged in described through hole one, described second electrode comprises the body of rod and cap body, one end and the described cap body of the described body of rod are connected, the described body of rod the other end can be electrically connected by the opening of described chock plug and outside, described elastic component is socketed on body of rod outer side, the outer end of described chock plug has along the radially inner gear edge of through hole one, the outer face of described elastic component is resisted against gear along upper, and the inner face of this elastic component is resisted against on cap body.Elastic component adopts rubber-plastics material to make, and rubber-plastics material comprises plastics, rubber, silica gel etc.When chock plug is fixed on body end, the second electrode reclines mutually with the end face at the cavity hatch edge of body, and elastic component produces compression set, improves the stopping property of the second electrode and edge of opening end face.The body of rod of the second electrode can keep off opening and the external electrical connections on edge by chock plug, the body of rod of the second electrode can extend to the outside of chock plug to the second electrifying electrodes; Certain body of rod also can not extend to the outside of chock plug, but the electric connection contacts of chock plug outside is put in chock plug by the opening of chock plug and is electrically connected with the second electrode.Elastic component generally can make round, also can be incomplete round even other shapes, as long as flexible and can grafting second electrode.Elastic component can be an independently elastic component, is arranged in an interference fit in through hole one, or is arranged in through hole one in the mode such as bonding and is connected with chock plug.
In above-mentioned electric shock pipe, alternatively, described chock plug comprises the tubular portion with through hole one and the mount pad being in a tubular form positioned at tubular portion, the second above-mentioned electrode to be slidably connected on mount pad and to have the elastic component of annular at mount pad overcoat, the height of elastic component is greater than the height of mount pad, described second electrode comprises the body of rod and cap body, the inner and the described cap body of the described body of rod are connected, the inner and the described cap body of the described body of rod are connected, the outer end of the described body of rod can be electrically connected by the opening of described chock plug and outside, the outer end of the described body of rod has the convex edge extended along body of rod radial direction, the size of described convex edge is slightly larger than the opening of described chock plug, the opening part of described chock plug has the abutment face leant with described convex edge, described elastic component between described tubular portion and mount pad, when chock plug is fixed on body end described elastic component produce compressive set make the outer face of elastic component and chock plug against, the inner face of elastic component and cap body against.Elastic component, between the tubular portion and mount pad of chock plug, can not come off, and chock plug and the second electrode lean with the two ends of elastic component respectively and can realize sealing, convenient install while ensure that sealing effectiveness.
In above-mentioned electric shock pipe, described elastic component is set on described mount pad outer side and the medial surface of described elastic component contacts with the outer side of mount pad.The medial surface of elastic component and upper surface contact against forming multidimensional seal with plug and are connected, and add that the inner face of elastic component and the second electrode are formed and are tightly connected, thus improve sealing effectiveness further.
In technique scheme, the first electrode is multistage cylinder or cone column, and the circumference along the first electrode on the upper end outer side wall of the first electrode has annular protrusion, and the inwall of described annular protrusion and described cavity forms the wringing fit sealed.The outer side of described convex shoulder and the tube wall of extension can leave some spaces and conveniently install.Formed by the annular protrusion of local and the inwall of cavity and seal, conveniently can install, reduce resistance during placement the first electrode.In the technical program, the length of extension is 1/3 ~ 2/3 of whole tube length.
Compared with prior art, the body portion be positioned in the utility model on the downside of the first electrode is the extension that can prevent the first electrode and the second electrode from producing high-voltage arc outside body, the good insulation preformance of extension, first electrode and the second electrode to produce high-voltage arc outside body, can only from walking around extension, by arranging extension, the first electrode and the second interelectrode air breakdown distance are lengthened greatly, even if be applied with very high high-voltage, also can effectively prevent from occurring punch-through in the first electrode and the air of the second electrode outside body.Thus ensure that electricimpulse realizes electroporation to the target liq sample in cavity.
Accompanying drawing explanation
Fig. 1 is that in embodiment one, this electric shock manages perspective view when not installing the second electrode.
Fig. 2 is that in embodiment one, the sectional view after the second electrode installed by this electric shock pipe.
Fig. 3 is the partial enlarged drawing in embodiment one.
Fig. 4 is the partial enlarged drawing in embodiment two.
In figure, 1, body; 1a, opening; 1e, cavity; 1h, extension; 1i, step; 2, the first electrode; 21, convex shoulder; 22, Access Division; 23, contact part; 3, the second electrode; 31, the body of rod; 31a, convex edge; 32, cap body; 4, chock plug; 41, through hole one; 42, edge is kept off; 44, mount pad; 45, tubular portion; 46, abutment face; 5, elastic component; 30, flexible connecting member.
Embodiment
Be below specific embodiment of the utility model and by reference to the accompanying drawings, the technical solution of the utility model is further described, but the utility model be not limited to these embodiments.
Embodiment one:
As depicted in figs. 1 and 2, this electric shock pipe comprises chock plug 4 and integrated body 1, chock plug 4 is connected on body 1 by flexible connecting member 30, body 1 inside has the cavity 1e for holding target liq sample, one end of body 1 has the opening 1a be connected with cavity 1e, the second electrode 3 is provided with and the outer end of the second electrode 3 can be electrically connected by the opening of chock plug 4 and outside in chock plug 4, the inner face of the second electrode 3 can fit with the annular end face at opening 1a edge, middle part in body 1 is provided with the first electrode 2 be connected with cavity 1e, first electrode 2 is formed with body 1 and seals, body 1 part be positioned on the downside of the first electrode 2 is the extension 1h that can prevent the first electrode 2 and the second electrode 3 from producing high-voltage arc outside body 1.This electric shock pipe in use, in cavity 1e, the liquid sample of the material comprising cell and cell will be injected is filled in from the opening 1a of body 1, then chock plug 4 is fixed on body 1 end and the annular end face at the inner face of the second electrode 3 and body 1 opening 1a edge is fitted, first electrode 2 and the second electrode 3 all with liquid communication in cavity 1e, be electrically connected with the first electrode 2 in the extension 1h that around electrode terminal of insulation can extend into body 1, the outer end of the second electrode 3 can be electrically connected by the opening of chock plug 4 and outside, first electrode 2 is connected with the pulse power with the second electrode 3, electric discharge makes to form electric field in the cavity 1e of electric shock pipe, cytolemma is made to have certain permeability, thus the target substance in liquid sample is entered in cell.In the technical program, body 1 part be positioned on the downside of the first electrode 2 is the extension 1h that can prevent the first electrode 2 and the second electrode 3 from producing high-voltage arc outside body 1, the good insulation preformance of extension 1h, first electrode 2 and the second electrode 3 to produce high-voltage arc outside body 1, at least will double the distance of extension 1h, or extension 1h can only be walked around, increase the distance of twice extension 1h, by arranging extension 1h, the distance of the air breakdown between the first electrode 2 and the second electrode 3 is lengthened greatly, even if be applied with very high high-voltage, also can effectively prevent from occurring punch-through in the first electrode 2 and the air of the second electrode 3 outside body 1.Thus ensure that electric current realizes electroporation to the target liq sample in cavity 1e; In addition, this extension 1h also has the effect as handle, facilitates this electric shock pipe to place and installs.
As shown in Figure 2, the length effect of extension 1h is larger, such as when the length of extension 1h is greater than the length of cavity 1e and insulate outside electrode terminal, the path distance that first electrode 2 and the second electrode 3 reach air breakdown outside body 1 is at least the length of the cavity 1e of three times, and such first electrode 2 and the second electrode 3 may produce high-voltage arc hardly outside body 1; Body 1 adopts the thickness of pipe that thickness of pipe that is made of plastic and extension 1h is less than cavity 1e place; The thickness of pipe at cavity 1e place is thicker, prevent the high-voltage breakdown between the first electrode 2 and the second electrode 3, the thickness of pipe that simultaneously thickness of pipe of extension 1h is less than cavity 1e place is convenient to the first electrode 2 and is extend in body 1 from extension 1h and be arranged on the middle part of body 1, is also convenient to electrode terminal and extend into that the extension 1h of body 1 is interior to be electrically connected with the first electrode 2; Body 1 is greater than body 1 at the pore diameter at cavity 1e place at the pore diameter at extension 1h place and forms step 1i in body 1, the Access Division 22 that second electrode 3 has convex shoulder 21 and is positioned on the upside of convex shoulder 21, the end face of convex shoulder 21 contacts with above-mentioned step 1i, Access Division 22 is connected in the pore at cavity 1e place, step 1i and convex shoulder 21 cooperatively interact and can play the effect of positioning limit, improve while convenience is installed and ensure stopping property, prevent target liq sample from leaking.
As shown in Figure 2, the second electrode 3 on the downside of convex shoulder 21 is contact part 23, and the diameter of contact part 23 is less than the diameter of convex shoulder 21 and contact part 23 exists gap with the tube wall of body 1 extension 1h, is also convenient to place the clamping of the second electrode 3 and install by contact part 23.The length of contact segment is general shorter, prevents the first electrode 2 too near apart from the lower end of extension 1h and walks around extension 1h generation electric arc.
As shown in Figure 3, be provided with the elastic component 5 be connected with the second electrode 3 in chock plug 4, the outside of elastic component 5 is connected with chock plug 4, and the inner side of elastic component 5 is connected with the second electrode 3; Chock plug 4 comprises the tubular portion 45 with through hole 1, second electrode 3 is arranged in through hole 1, second electrode 3 comprises the body of rod 31 and cap body 32, one end and the cap body 32 of the body of rod 31 are connected, the body of rod 31 the other end can be electrically connected by the opening of chock plug 4 and outside, and elastic component 5 is socketed on the body of rod 31 outer side, and the outer end of chock plug 4 has along the radially inner gear of through hole 1 along 42, the outer face of elastic component 5 is resisted against gear along on 42, and the inner face of this elastic component 5 is resisted against on cap body 32.When chock plug 4 is fixed on body 1 end, elastic component 5 produces compressive set, elastic component 5 is formed with chock plug 4 and the second electrode 3 seal, can avoid occurring gap between the second electrode 3 and the opening 1a of body 1, air is avoided to enter liquid sample in cavity 1e, and, when slightly upwarping appears in chock plug 4 time, elastic component 5 also can do certain recovery deformation, ensure that the second electrode 3 still reclines mutually with the end face at opening 1a edge, thus make the liquid sample in cavity 1e still can not produce air bubble when experimenter's application of sample operates some deviation; To sum up, the technical program improves the sealing property between the second electrode 3 and opening 1a effectively, thus avoids the air in the external world when adding liquid sample to enter in cavity 1e.
Elastic component 5 adopts rubber-plastics material to make, and rubber-plastics material comprises plastics, rubber, silica gel etc.When chock plug 4 is fixed on body 1 end, the second electrode 3 reclines mutually with the end face at the cavity 1e opening 1a edge of body 1, and elastic component 5 produces compression set, improves the stopping property of the second electrode 3 and opening 1a edge end face.The body of rod 31 of the second electrode 3 can by chock plug 4 keep off along 42 opening 1a and external electrical connections, the outside that the body of rod 31 of the second electrode 3 can extend to chock plug 4 is energized to the second electrode 3; Certain body of rod 31 also can not extend to the outside of chock plug 4, but the electric connection contacts of chock plug 4 outside is put in by the opening of chock plug 4, and chock plug 4 is interior to be electrically connected with the second electrode 3.Elastic component 5 generally can make round, also can be incomplete round even other shapes, as long as flexible and can grafting second electrode 3.Elastic component 5 can be an independently elastic component 5, is arranged in an interference fit in through hole 1, or is arranged in through hole 1 in the mode such as bonding and is connected with chock plug 4.
In the present embodiment, the first electrode 2 is multistage cylinder or cone column, and further, the upper end outer side wall of the first electrode 2 has annular protrusion along the circumference of the first electrode 2, the inwall of annular protrusion and cavity 1e forms the wringing fit sealed.The outer side of convex shoulder 21 and the tube wall of extension 1h can leave some spaces and conveniently install.Formed by the annular protrusion of local and the inwall of cavity 1e and seal, conveniently can install, reduce resistance during placement the first electrode 2.
Body 1 in the present embodiment, chock plug 4 all adopt insulating material to make, and the first electrode 2 and the second electrode 3 adopt electro-conductive material to make, and this part is prior art, and not in this to go forth for the material of embody rule.In the present embodiment, the length of extension 1h is 1/3 ~ 2/3 of whole body 1 length.In addition, the first electrode 2 both can directly be fixed in body 1, also equally with the second electrode 3 can be arranged on inside a chock plug 4 and seal body 1 cavity 1e.
Embodiment two:
As shown in Figure 4, the present embodiment is roughly the same with embodiment one, difference is, in the present embodiment, chock plug 4 comprises the tubular portion 45 with through hole 1 and the mount pad 44 being in a tubular form positioned at tubular portion 45, the second above-mentioned electrode 3 to be slidably connected on mount pad 44 and to have the elastic component 5 of annular at mount pad 44 overcoat, the height of elastic component 5 is greater than the height of mount pad 44, when chock plug 4 being fixed on body 1 end elastic component 5 produce compressive set make the outer face of elastic component 5 and chock plug 4 against, the inner face of elastic component 5 and the second electrode 3 against, elastic component 5 is set on mount pad 44 outer side and the medial surface of elastic component 5 contacts with the outer side of mount pad 44.Between the tubular portion 45 of elastic component 5 chock plug 4 and mount pad 44, can not come off, and chock plug 4 and the second electrode 3 lean with the two ends of elastic component 5 respectively and can realize sealing, the medial surface of elastic component 5 and upper surface contact against forming multidimensional seal with plug and are connected, add that the lower surface of elastic component 5 and the second electrode 3 are formed to be tightly connected, convenient install while ensure that sealing effectiveness.
The outer end of the body of rod 31 of the second electrode 3 has the convex edge 31a extended along the body of rod 31 radial direction, and the size of convex edge 31a is slightly larger than the opening of chock plug 4, and the opening part of chock plug 4 has the abutment face 46 leant with convex edge 31a; When chock plug 4 is fixed on body 1 end elastic component 5 produce compressive set make the outer face of elastic component 5 and chock plug 4 against, the inner face of elastic component 5 and cap body 32 against.Convex edge 31a can pass chock plug 4 smoothly and lean with the abutment face 46 of chock plug 4 opening part after external force extrusion plug capitiform becomes, and after mount pad 44 recovers deformation, convex edge 31a matches with abutment face 46 to be formed and keeps spacing, effectively prevents coming off of the second electrode 3.
Specific embodiment described in literary composition is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various amendment or supplements or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present utility model or surmount the scope that appended claims defines.
Claims (10)
1. an electric shock pipe, described electric shock pipe comprises chock plug (4) and integrated body (1), described body (1) inside has the cavity (1e) for holding target liq sample, one end of described body (1) has the opening (1a) be connected with described cavity (1e), the second electrode (3) is provided with and the outer end of described second electrode (3) can be electrically connected by the opening of described chock plug (4) and outside in described chock plug (4), the inner face of described second electrode (3) can fit with the annular end face at opening (1a) edge, it is characterized in that, middle part in described body (1) is provided with the first electrode (2) be connected with cavity (1e), described first electrode (2) and body (1) are formed and seal, described body (1) part being positioned at the first electrode (2) downside is to prevent the first electrode (2) and the second electrode (3) from producing the extension (1h) of high-voltage arc in described body (1) outside.
2. electric shock pipe according to claim 1, it is characterized in that, the length of described extension (1h) is between 1mm and 40mm.
3. electric shock pipe according to claim 2, it is characterized in that, the length of described extension (1h) is between 2mm and 30mm.
4. electric shock pipe according to claim 1 and 2, it is characterized in that, described body (1) is greater than described body (1) at the pore diameter at described cavity (1e) place and at body (1) middle formation step (1i) at the pore diameter at extension (1h) place, described the second electrode (3) has convex shoulder (21) and is positioned at the Access Division (22) of convex shoulder (21) upside, the end face of described convex shoulder (21) contacts with above-mentioned step (1i), described Access Division (22) is connected in the pore at described cavity (1e) place.
5. electric shock pipe according to claim 4, it is characterized in that, convex shoulder (21) downside of described second electrode (3) is provided with contact part (23), and the diameter of described contact part (23) is less than the diameter of convex shoulder (21) and contact part (23) exists gap with the tube wall of described body (1) extension (1h).
6. electric shock pipe according to claim 1 and 2, it is characterized in that, elastic component (5) is provided with between described chock plug (4) and the second electrode (3), the outer end of described elastic component (5) or outside and described chock plug (4) can be formed and be tightly connected, and the inner of described elastic component (5) or inner side can be formed with described second electrode (3) and be tightly connected.
7. electric shock pipe according to claim 6, it is characterized in that, described chock plug (4) comprises the tubular portion (45) with through hole one (41), described second electrode (3) is arranged in described through hole one (41), described second electrode (3) comprises the body of rod (31) and cap body (32), one end and the described cap body (32) of the described body of rod (31) are connected, the described body of rod (31) the other end can be electrically connected by the opening of described chock plug (4) and outside, described elastic component (5) is socketed on the body of rod (31) outer side, the outer end of described chock plug (4) has along the radially inner gear of through hole one (41) along (42), the outer face of described elastic component (5) is resisted against gear on (42), the inner face of this elastic component (5) is resisted against on cap body (32).
8. electric shock pipe according to claim 1 and 2, it is characterized in that, described second electrode (3) comprises the body of rod (31) and cap body (32), the inner and the described cap body (32) of the described body of rod (31) are connected, the described body of rod (31) to be plugged in described chock plug (4) and to be slidably connected with described chock plug (4), the inner and the described cap body (32) of the described body of rod (31) are connected, the outer end of the described body of rod (31) can be electrically connected by the opening of described chock plug (4) and outside, the outer end of the described body of rod (31) has the convex edge (31a) extended along the body of rod (31) radial direction, the size of described convex edge (31a) is slightly larger than the opening of described chock plug (4), the opening part of described chock plug (4) has the abutment face (46) leant with described convex edge (31a), elastic component (5) is provided with between described chock plug (4) and described cap body (32), when chock plug (4) is fixed on body (1) end described elastic component (5) produce compressive set make the outer face of elastic component (5) and chock plug (4) against, the inner face of elastic component (5) and cap body (32) against.
9. electric shock pipe according to claim 8, it is characterized in that, described chock plug (4) comprises the tubular portion (45) with through hole one (41) and the mount pad (44) being in a tubular form positioned at tubular portion (45), above-mentioned the second electrode (3) is slidably connected on mount pad (44), and described elastic component (5) is positioned between described tubular portion (45) and mount pad (44) and the height of described elastic component (5) is greater than the height of mount pad (44).
10. electric shock pipe according to claim 9, it is characterized in that, described elastic component (5) is set on described mount pad (44) outer side and the medial surface of described elastic component (5) contacts with the outer side of mount pad (44).
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981477.2U CN205205151U (en) | 2015-12-01 | 2015-12-01 | Electric shock pipe |
| JP2017530204A JP6629858B2 (en) | 2014-12-02 | 2015-12-02 | Electric shock pipe and electroporation apparatus having the electric shock pipe |
| PCT/IB2015/059297 WO2016088062A1 (en) | 2014-12-02 | 2015-12-02 | An electric shock pipe and cell electroporator having electric shock pipe |
| US15/532,182 US10472601B2 (en) | 2014-12-02 | 2015-12-02 | Shock tube and cell electroporation device with the shock tube |
| EP15864958.2A EP3228695A4 (en) | 2014-12-02 | 2015-12-02 | An electric shock pipe and cell electroporator having electric shock pipe |
| US16/586,989 US11965149B2 (en) | 2014-12-02 | 2019-09-29 | Shock tube and cell electroporation device with the shock tube |
| JP2019219959A JP6838125B2 (en) | 2014-12-02 | 2019-12-04 | An electric shock pipe and an electroporation device having the electric shock pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981477.2U CN205205151U (en) | 2015-12-01 | 2015-12-01 | Electric shock pipe |
Publications (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110885857A (en) * | 2018-09-11 | 2020-03-17 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110885857A (en) * | 2018-09-11 | 2020-03-17 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
| CN110885857B (en) * | 2018-09-11 | 2024-01-26 | 苏州壹达生物科技有限公司 | Intermittent flow type formula electrotransfection device |
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