CN205170815U - Biological cell fixing device - Google Patents
Biological cell fixing device Download PDFInfo
- Publication number
- CN205170815U CN205170815U CN201520814031.0U CN201520814031U CN205170815U CN 205170815 U CN205170815 U CN 205170815U CN 201520814031 U CN201520814031 U CN 201520814031U CN 205170815 U CN205170815 U CN 205170815U
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- CN
- China
- Prior art keywords
- microflute
- cell
- utility
- model
- stationary installation
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Abstract
The utility model relates to a biological cell fixing device, including the microflute that basement and the array that is located the substrate surface set up, every microflute includes that a tank bottom face and ring establish the cell wall face that inclines at the tank bottom face all around, every cell wall face of microflute is the inclined plane, just the cell wall face of microflute is the obtuse angle setting with the contained angle of tank bottom face. The utility model discloses a microflute of array setting is caught and the fixed cell, can guarantee that the cell gets into the microflute along the tipper wall of microflute under the condition of not damaged in, improve the cell capture rate, realize convenient, swift cell is placed with fixed, the utility model discloses simple structure can simplify the cell and operate regularly, alleviates operating personnel's the work degree of difficulty.
Description
Technical field
The utility model relates to biology and medical field.Especially, the utility model relates to a kind of biomass cells stationary installation.
Background technology
In field of reproduction, often relate to the relevant treatment to biological ovum, as passed through fixing to puncture, to find time ovum.In the research of zebra fish-egg, one of the most frequently used technology is exactly microinjection; Namely under the microscope, zebra fish ovum is fixed in square fence that agar material surrounds, by pressure, the substance injection in syringe needle is entered in cell.Whole injection process carries out under powerful microscope, therefore requires that the stationary installation of ovum and operator have high technical requirements.
In prior art, need small zebra fish ovum gripping, transport and be precisely placed in object fence, consume operator's a large amount of time and energy; And due to ovum less and more fragile, general equipment or apparatus are difficult to fix preferably, and operation or place unreasonable its tissue of equal destructible or damaging cells, bring difficulty to analysis.Therefore, how to realize not in damaging cells situation, fast, easily ovum place and be fixedly one of current research topic.
Utility model content
The utility model aims to provide a kind of biomass cells stationary installation, with large, the inefficient problem of placement and fixing operation difficulty solving cell in existing microinjection.
The utility model discloses a kind of biomass cells stationary installation, concrete scheme is as follows: the microflute that the array that this cell stationary installation comprises substrate and is positioned at substrate surface is arranged, each microflute comprises the trough wall surface that a groove bottom and ring are located at groove bottom surrounding side, each trough wall surface of described microflute is scarp, and the angle of the trough wall surface of described microflute and bottom surface is obtuse angle arranges.
Further, described substrate and described microflute are integral forming structure.
Further, the cross section of described microflute is trilateral, rhombus, square or regular hexagon.
Further, the vertical section of the trough wall surface of described microflute is trilateral, rounded triangle or trapezoidal.
Further, the trough wall surface of described microflute and the angle of groove bottom are 120 °-150 °.
Further, described substrate and microflute are polydimethyl siloxane material.
The microflute that the utility model adopts array to arrange is caught and fixed cell, can ensure that cell enters in microflute along tipper wall in undamaged situation, improves cell capture efficiency, realizes cell easily and efficiently and places with fixing; The utility model structure is simple, can simplify cell and operate regularly, alleviate the work difficulty of operator.
Accompanying drawing explanation
Fig. 1 is the structure iron of the cross section of cell stationary installation of the present utility model;
Fig. 2 is the partial longitudinal section figure of cell stationary installation of the present utility model.
Embodiment
For further illustrating each embodiment, the utility model provides accompanying drawing.These accompanying drawings are a part for the utility model disclosure, and it is mainly in order to illustrate embodiment, and the associated description of specification sheets can be coordinated to explain the operation principles of embodiment.Coordinate with reference to these contents, those of ordinary skill in the art will be understood that other possible embodiments and advantage of the present utility model.Assembly in figure not drawn on scale, and similar element numbers is commonly used to assembly like representation class.
Now with embodiment, the utility model is further illustrated by reference to the accompanying drawings.
As depicted in figs. 1 and 2, the microflute 2 that biomass cells stationary installation of the present utility model is arranged by substrate 1 and the array being positioned at substrate 1 surface forms, each microflute 2 comprises the trough wall surface 3 that a groove bottom (not shown) and ring are located at groove bottom surrounding side, each trough wall surface 3 of this microflute 2 is scarp, and the trough wall surface 3 of described microflute 2 is that obtuse angle is arranged with the angle of groove bottom.
In the present embodiment, this substrate 1 and microflute 2 are integral forming structure, and material therefor is polydimethyl siloxane material.
In the present embodiment, the cross section of this microflute 2 is square, is arranged in array on the base 1.Microflute cross section of the present utility model is not limited to square, can also be trilateral, rhombus or regular hexagon.
In the present embodiment, the vertical section of this trough wall surface 3 is trilateral.Of course, in other embodiments of the present utility model, the vertical section of trough wall surface 3 can also be rounded triangle or trapezoidal.
In the present embodiment, the trough wall surface 3 of microflute 2 is designed to 120 ° with the angle of the groove bottom of microflute 2.Under this angle design, when using suction pipe cell spray to be placed in cell stationary installation of the present utility model, cell can slip in microflute automatically along the wall of microflute, realizes the efficient capture of cell, simultaneously not damaging cells tissue.In other embodiments of the present utility model, the angle of the trough wall surface 3 of microflute 2 and the groove bottom of microflute 2 can be set to the unspecified angle between 120 °-150 °, and all can realize the object of automatically catching cell, difference is only the capture rate of cell.
The utility model is a kind of array microflute cell stationary installation, cell and water mixture is drawn with suction pipe, drop in the surface of stationary installation, along with the ejection of suction pipe, cell dispersal, in different zones, then siphons away unnecessary water droplet, keep cell surface moistening full, now cell can slip into microflute 2 inside along the tipper wall 3 of microflute 2, thus improves cell capture efficiency under the prerequisite ensureing not damaged cell, realizes cell easily and efficiently and places with fixing.
Although specifically show in conjunction with preferred embodiment and describe the utility model; but those skilled in the art should be understood that; not departing from the spirit and scope of the present utility model that appended claims limits; can make a variety of changes the utility model in the form and details, be protection domain of the present utility model.
Claims (6)
1. a biomass cells stationary installation, it is characterized in that: the microflute that the array comprising substrate and be positioned at substrate surface is arranged, each microflute comprises the trough wall surface that a groove bottom and ring are located at groove bottom surrounding side, each trough wall surface of described microflute is scarp, and the angle of the trough wall surface of described microflute and groove bottom is obtuse angle arranges.
2. a kind of biomass cells stationary installation according to claim 1, is characterized in that: described substrate and described microflute are integral forming structure.
3. a kind of biomass cells stationary installation according to claim 1, is characterized in that: the cross section of described microflute is trilateral, rhombus, square or regular hexagon.
4. a kind of biomass cells stationary installation according to claim 1, is characterized in that: the vertical section of the trough wall surface of described microflute is trilateral, rounded triangle or trapezoidal.
5. a kind of biomass cells stationary installation according to claim 1, is characterized in that: the trough wall surface of described microflute and the angle of groove bottom are 120 °-150 °.
6. a kind of biomass cells stationary installation according to claim 1, is characterized in that: described substrate and microflute are made up of polydimethyl siloxane material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520814031.0U CN205170815U (en) | 2015-10-21 | 2015-10-21 | Biological cell fixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520814031.0U CN205170815U (en) | 2015-10-21 | 2015-10-21 | Biological cell fixing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205170815U true CN205170815U (en) | 2016-04-20 |
Family
ID=55734829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520814031.0U Expired - Fee Related CN205170815U (en) | 2015-10-21 | 2015-10-21 | Biological cell fixing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205170815U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520748A (en) * | 2016-06-30 | 2017-03-22 | 厦门大学 | Cell fixation material suitable for microinjection, and preparation method thereof |
-
2015
- 2015-10-21 CN CN201520814031.0U patent/CN205170815U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520748A (en) * | 2016-06-30 | 2017-03-22 | 厦门大学 | Cell fixation material suitable for microinjection, and preparation method thereof |
| CN106520748B (en) * | 2016-06-30 | 2020-08-21 | 厦门大学 | Material suitable for cell fixation of microinjection and preparation method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20171021 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |