CN205109101U - Chromatography device - Google Patents
Chromatography device Download PDFInfo
- Publication number
- CN205109101U CN205109101U CN201520891179.4U CN201520891179U CN205109101U CN 205109101 U CN205109101 U CN 205109101U CN 201520891179 U CN201520891179 U CN 201520891179U CN 205109101 U CN205109101 U CN 205109101U
- Authority
- CN
- China
- Prior art keywords
- piston
- chromatographic
- chromatographic column
- chromatography
- cock body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004587 chromatography analysis Methods 0.000 title abstract description 27
- 239000000945 filler Substances 0.000 claims description 17
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 2
- 239000003480 eluent Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 8
- 238000000746 purification Methods 0.000 abstract description 6
- 238000002347 injection Methods 0.000 abstract description 3
- 239000007924 injection Substances 0.000 abstract description 3
- 238000001042 affinity chromatography Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 16
- 238000011049 filling Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model provides a chromatography device belongs to biological purification chromatography device field, and present device structure is complicated in order to solve, the high scheduling problem design of manufacturing cost. This chromatography device includes the chromatography column, and chromatography column upper end opening, lower extreme are connected with the filter, gets into and can follow the chromatography column inner wall and slide and be provided with piston assembly from the chromatography column open end, and piston assembly includes piston cock body and piston rod, the piston rod run through the piston cock body and with piston cock body fixed connection, the runner that runs through is seted up to the piston rod on its axial direction, the runner communicates with the cavity that piston cock body and chromatography column lower extreme formed. The utility model discloses an injection of treating chromatography liquid has been realized to chromatography device's piston assembly's piston rod, and quick affinity chromatography is realized to the piston assembly liquid in the chromatography column that still is used for pressurizeing, the power pump that has saved present device with inject the pipeline into, under the prerequisite of guaranteeing the chromatography quality, it is simpler to have the structure, prepares advantage with low costs.
Description
Technical field
The utility model relates to biological purification chromatographic technique field, particularly relates to a kind of chromatographic apparatus.
Background technology
Chromatographic technique bio-molecular separation with analyze in apply very extensive, can efficiently, separate targets thing or remove impurity, also for sample concentration, purification or solution replacement etc. from mixture rapidly.Affinity chromatography is the one of chromatographic technique, can be placed in chromatographic column with the filler of object specific binding (filler as affine in ProteinA, ProteinG is affine filler, the affine filler of metal-chelating etc.), when sample solution is by chromatographic column, affine filler specific binding in object and chromatographic column and be trapped in chromatographic column, other are not directly flowed out by the molecule adsorbed, by selecting suitable eluant solution chromatographic column combined Protein elution can be gone out, thus the separation of realize target molecule.
Existing chromatographic column comprises common chromatographic column and gravity post, and common chromatographic column need configure extra kinetic pump device to carrying out chromatographic runs, could have certain requirement to equipment performance; Chromatographic column flow velocity is low, and flux is little, and purification chromatography efficiency is low.
Utility model content
The utility model provides a kind of chromatographic apparatus, and its object is to provides the chromatographic apparatus that a kind of structure is more simple, chromatography efficiency is higher.
For reaching this object, the utility model by the following technical solutions:
The utility model provides a kind of chromatographic apparatus, comprise chromatographic column, described chromatographic column upper end open, lower end is connected with filter, enter from described chromatographic column openend and can slide along chromatographic column inwall and be provided with piston component, described piston component comprises piston cock body and piston rod, described piston rod runs through described piston cock body and is fixedly connected with piston cock body, described piston rod offers the runner run through in its axial direction, the chamber that described runner and piston cock body and chromatographic column lower end are formed, for treating that chromatographic solution is injected in chromatographic column.
Further technical scheme is, one end inwall of described piston rod connection piston cock body is provided with check-valves.
Further technical scheme is, described chromatographic column inwall and open end is provided with limiting stand.
Further technical scheme is, before the described piston component of loading, injects the affine filler of setting volume in chromatographic column, described affine filler can with treat that the object in chromatographic solution is combined.
Further technical scheme is, the side that described piston rod does not connect piston cock body is fixedly installed draw back plate, and described draw back plate is provided with through hole, described draw back plate is also provided with the buckle closure for fastening or open described through hole.
Further technical scheme is, described buckle closure one end and described draw back plate hinged.
Further technical scheme is, described chromatographic column outer wall is provided with for measuring affine filler and the scale treating chromatographic solution injected slurry volume.
Further technical scheme is, the filter membrane of described filter interior is hydrophylic filter membranes, and the filtering accuracy of described filter membrane is 0.45um, and the capacity of described chromatographic column is 25ml.
The beneficial effects of the utility model are:
The piston rod of the piston component of chromatographic apparatus of the present utility model achieves the injection treating chromatographic solution, piston component is also for liquid in pressurizing chromatographic column, achieve pressurization and feed liquor carry out simultaneously, eliminate the kinetic pump of existing apparatus and independent filling line, under the prerequisite ensureing chromatography quality, there is the advantage that structure is more simple, preparation cost is low, purification chromatography efficiency is high.
Accompanying drawing explanation
The structural representation of the chromatographic apparatus that Fig. 1 provides for the utility model preferred embodiment.
In figure:
1, chromatographic column; 2, filter; 3, piston component; 4, runner; 5, check-valves; 6, limiting stand; 7, draw back plate; 8, through hole; 9, buckle closure; 10, filter membrane; 31, piston cock body; 32, piston rod.
Detailed description of the invention
The technical solution of the utility model is further illustrated by detailed description of the invention below in conjunction with accompanying drawing.
Preferred embodiment
Present embodiments provide a kind of chromatographic apparatus, as shown in Figure 1, comprise chromatographic column 1, chromatographic column 1 is hollow columnar structures, its upper end open, and lower end is outlet end, and this fluid port is connected with filter 2.Enter from chromatographic column 1 openend and can slide along chromatographic column 1 inwall and be provided with piston component 3, piston component 3 comprises piston cock body 31 and piston rod 32.Piston cock body 31 is elastic component, can gapless press-in in chromatographic column 1.Piston rod 32 runs through piston cock body 31 and is fixedly connected with piston cock body 31, piston rod 32 offers the runner 4 run through in its axial direction, the chamber that runner 4 is formed with piston cock body 31 and chromatographic column 1 lower end, for treating that chromatographic solution is injected in chromatographic column 1.
Above-mentioned chromatographic column 1 is used to carry out treating that the process of chromatographic solution chromatography is as follows:
Step1: under not loading the prerequisite of piston component 3, injects the affine filler of setting volume in chromatographic column 1;
Step2: load piston component 3 from chromatographic column 1 openend, piston cock body 31 is introduced into reserved setting height between chromatographic column 1 to piston cock body 31 and filler liquid level, and this setting height is determined according to by the metering treating chromatographic solution of injecting;
Step3: inject in the runner 4 of piston rod 32 and treat chromatographic solution, treats that chromatographic solution to enter in the chamber that piston cock body 31 and chromatographic column 1 lower end formed through runner 4 and is combined with affine filler;
Step4: slowly promote piston rod 32 downwards, to the liquid pressing in chromatographic column 1, make to treat that chromatographic solution is fully combined fast with affine filler, treat that the object of chromatography is combined with affine filler and is trapped in chromatographic column 1, other are not flowed out from the port of export by the molecule adsorbed.
Preferably, in piston rod 32 pressure process, in order to prevent liquid self-flowing road 4 reflux in chromatographic column 1 from affecting chromatography efficiency and chromatography quality, one end inwall connecting piston cock body 31 at piston rod 32 is provided with check-valves 5.5, this check-valves allows liquid self-flowing road 4 to flow in the chamber of lower end.
Preferably, for preventing causing chromatographic column 1 to be toppled in chromatography midway because of maloperation, cause piston component 3 to skid off in chromatographic column 1, chromatographic column 1 inwall is provided with limiting stand 6 near open end.
Preferably, for making the push-and-pull of piston rod 32 more laborsaving, the side that piston rod 32 does not connect piston cock body 31 is fixedly installed draw back plate 7, draw back plate 7 is provided with through hole 8, draw back plate 7 is also provided with the buckle closure 9 for fastening or open through hole 8.Buckle closure 9 one end and draw back plate 7 hinged.
In order to the affine filler of accurate measurement more and the volume treating chromatographic solution, chromatographic column 1 outer wall is decorated with scale.
Preferably, chromatographic column 1 can select capacity to be 25ml syringe, and filter 2 can select the filtering accuracy of filter membrane 10 to be the hydrophilic syringe needle filter of 0.45um.
The piston rod 32 of the piston component 3 of the chromatographic apparatus of the present embodiment achieves the injection treating chromatographic solution, piston component 3 is also for liquid in pressurizing chromatographic column, achieve pressurization and feed liquor carry out simultaneously, eliminate the kinetic pump of existing apparatus and independent filling line, under the prerequisite ensureing chromatography quality, there is the advantage that structure is more simple, preparation cost is low, purification chromatography efficiency is high.
Below know-why of the present utility model is described in conjunction with specific embodiments.These describe just in order to explain principle of the present utility model, and can not be interpreted as the restriction to the utility model protection domain by any way.Based on explanation herein, those skilled in the art does not need to pay performing creative labour can associate other detailed description of the invention of the present utility model, and these modes all will fall within protection domain of the present utility model.
Claims (8)
1. a chromatographic apparatus, comprise chromatographic column (1), described chromatographic column upper end open, lower end is connected with filter (2), it is characterized in that: enter from described chromatographic column (1) openend and the slip of chromatographic column (1) inwall can be provided with piston component (3), described piston component (3) comprises piston cock body (31) and piston rod (32), described piston rod (32) runs through described piston cock body (31) and is fixedly connected with piston cock body (31), described piston rod (32) offers the runner (4) run through in its axial direction, the chamber that described runner (4) is formed with piston cock body (31) and chromatographic column (1) lower end, for treating that chromatographic solution or eluent are injected in chromatographic column (1).
2. chromatographic apparatus according to claim 1, is characterized in that: one end inwall of described piston rod (32) connection piston cock body (31) is provided with check-valves (5).
3. chromatographic apparatus according to claim 1 and 2, is characterized in that: on described chromatographic column (1) inwall and near open end, be provided with limiting stand (6).
4. chromatographic apparatus according to claim 1, is characterized in that: front in the described piston component of loading (3), injects the affine filler of setting volume in chromatographic column (1), described affine filler can with treat that the object in chromatographic solution is combined.
5. chromatographic apparatus according to claim 1, it is characterized in that: the side that described piston rod (32) does not connect piston cock body (31) is fixedly installed draw back plate (7), described draw back plate (7) is provided with through hole (8), described draw back plate (7) is also provided with the buckle closure (9) for fastening or open described through hole (8).
6. chromatographic apparatus according to claim 5, is characterized in that: described buckle closure (9) one end and described draw back plate (7) hinged.
7. chromatographic apparatus according to claim 4, is characterized in that: described chromatographic column (1) outer wall is provided with for measuring affine filler and the scale treating chromatographic solution injected slurry volume.
8. chromatographic apparatus according to claim 1, it is characterized in that: the filter membrane (10) of described filter (2) inside is hydrophylic filter membranes, the filtering accuracy of described filter membrane is 0.45um, and the capacity of described chromatographic column (1) is 25ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520891179.4U CN205109101U (en) | 2015-11-10 | 2015-11-10 | Chromatography device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520891179.4U CN205109101U (en) | 2015-11-10 | 2015-11-10 | Chromatography device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205109101U true CN205109101U (en) | 2016-03-30 |
Family
ID=55564750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520891179.4U Expired - Fee Related CN205109101U (en) | 2015-11-10 | 2015-11-10 | Chromatography device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205109101U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114072216A (en) * | 2019-06-28 | 2022-02-18 | 信达生物制药(苏州)有限公司 | Modular chromatography device |
| CN115105859A (en) * | 2022-06-08 | 2022-09-27 | 上海金鑫生物科技有限公司 | Self-adaptive sealed chromatographic column |
-
2015
- 2015-11-10 CN CN201520891179.4U patent/CN205109101U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114072216A (en) * | 2019-06-28 | 2022-02-18 | 信达生物制药(苏州)有限公司 | Modular chromatography device |
| CN115105859A (en) * | 2022-06-08 | 2022-09-27 | 上海金鑫生物科技有限公司 | Self-adaptive sealed chromatographic column |
| CN115105859B (en) * | 2022-06-08 | 2024-03-19 | 上海金鑫生物科技有限公司 | Self-adaptive sealed chromatographic column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160330 |