CN205091342U - Immunity magnetic separation device - Google Patents
Immunity magnetic separation device Download PDFInfo
- Publication number
- CN205091342U CN205091342U CN201520918181.6U CN201520918181U CN205091342U CN 205091342 U CN205091342 U CN 205091342U CN 201520918181 U CN201520918181 U CN 201520918181U CN 205091342 U CN205091342 U CN 205091342U
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- China
- Prior art keywords
- magnet
- separating device
- support arm
- magnetic separating
- position limit
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- 230000036039 immunity Effects 0.000 title abstract description 4
- 238000007885 magnetic separation Methods 0.000 title abstract 3
- 238000000926 separation method Methods 0.000 claims abstract description 32
- 230000003028 elevating effect Effects 0.000 claims abstract description 3
- 239000011324 bead Substances 0.000 claims description 28
- 239000006249 magnetic particle Substances 0.000 claims description 4
- 239000013077 target material Substances 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 230000005389 magnetism Effects 0.000 abstract 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 abstract 1
- 230000005622 photoelectricity Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 210000002381 plasma Anatomy 0.000 description 7
- 239000013076 target substance Substances 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000001042 affinity chromatography Methods 0.000 description 6
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 102000009027 Albumins Human genes 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 2
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 2
- 239000003114 blood coagulation factor Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102400001244 Cerebellin Human genes 0.000 description 1
- 101800001299 Cerebellin Proteins 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 229910000828 alnico Inorganic materials 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 229910000859 α-Fe Inorganic materials 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses an immunity magnetic separation device of the target material (such as biomolecule ) of separation high viscosity solution in such as plasma. The utility model provides an immunity magnetic separation device, the device includes: horizontal base, two columns installed on the pedestal. Elevating system is including linear slide rail, slider and toothed belt, magnetism separating mechanism is including magnet and magnet support arm, actuating mechanism is including step motor and photoelectricity stopper, a power supply. And control mechanism, including logic circuit controller and stepper motor controller. The utility model discloses can be safely, high -efficient ground and separate the target material of high viscosity solution in such as plasma fast, realize the large -scale production to this target material.
Description
Technical field
The utility model relates to a kind of immune magnetic separating device for separating of the target substance (such as biomolecule) in high viscosity solution such as blood plasma.
Background technology
Isolation of target substances such as albumin, clotting factor, CER, transferrin etc. from high viscosity solution such as blood plasma at present, most blood product enterprise all adopts cold ethanol to combine the method for chromatography to be separated these bioproteins in the world, but the trace of albumin that cold ethanol method produces plasma proteins process results is little, and the discarded object of generation contains a large amount of valuable composition.
For multiple blood coagulation class and anticoagulation series products, international large manufacturer many employings native ligand affinity chromatography or antibody affinity chromatography legal system standby.In addition, because the viscosity of blood plasma is high, therefore can affect velocity of separation to a certain extent in affinity chromatography, especially in large-scale production, need to optimize accordingly and special design the specificity etc. of affinity column, tomography devices, solid-phase adsorbent, affinity ligand.And China is due in the technical backwardness of extensive affinity chromatography, the high-purity clotting factor series products still not having affinity chromatography to prepare at present comes out.
For this reason, be the technical deficiency of shortcoming and affinity chromatography that overcomes cold ethanol method and shortcoming, need to develop and be a kind ofly suitable for being separated efficiently, with high yield various biomolecules or the method for separate targets biomolecule or device specifically from blood plasma.
Utility model content
In order to solve the problem, the utility model provides a kind of immune magnetic separating device, comprising: horizontal base; Column; Elevating mechanism, comprises line slide rail, slide block and odontoid belt; Magneto separate mechanism, comprises magnet and magnet support arm; Driving mechanism, comprises stepper motor and photoelectric position limit device; Power supply; And control gear, comprise logical circuit controller and controllor for step-by-step motor.
In the utility model, line slide rail is vertically fixed on column, and slide block can be lower mobile in the vertical direction on described line slide rail; Magnet support arm comprises free-end and stiff end, and magnet is fixed on the free-end of magnet support arm, and the stiff end of magnet support arm to be fixed on slide block and to be fixedly connected with odontoid belt; The driving wheel of stepper motor is connected with odontoid belt; And according to the signal that photoelectric position limit device sends, controllor for step-by-step motor sends instruction control step motor forward or reverse, thus control odontoid belt drives magnet support arm to move up and down on line slide rail.
In a preferred embodiment, Magneto separate mechanism can comprise two magnet support arms and two magnets, each magnet support arm is fixed a magnet.
In a preferred embodiment, photoelectric position limit device can comprise uplink and downlink photoelectric position limit device, and uplink and downlink photoelectric position limit device can be arranged on the upper and lower of described column respectively.
In a preferred embodiment, the separation container holding the solution containing magnetic particle can be placed between described two magnets, distance when placing described separation container between magnet and the chamber wall of separation container is less than 0.5 centimetre, is preferably less than 0.1-0.3 centimetre.
In the utility model, magnetic particle can be magnetic bead.
In a preferred embodiment, separation container can be cylindrical shape, can cover at least half of the girth of separation container at the inside surface of circumferentially two magnets of separation container.
In a preferred embodiment, magnet can be electromagnet.
In a further preferred embodiment, magnet can be permanent magnet.
In a preferred embodiment, described immune magnetic separating device may be used for the magnetic bead in separating high-viscosity solution, thus can be separated the target substance be combined on magnetic bead further.In a further preferred embodiment, described high viscosity solution can be blood plasma.
The beneficial effects of the utility model:
The utility model moves down along the chamber wall of separation container by making magnet, can under the influence of a magnetic field a part of magnetic bead be attracted on chamber wall, in the process simultaneously moved down at magnet, magnetic beads a large amount of under the impact of the magnetic line of force is thereupon with the downward sedimentation of identical speed, thus overcome the resistance of solution viscosity to magnetic bead sedimentation to a great extent, accelerate velocity of separation and the efficiency of magnetic bead.Owing to magnetic bead being combined with target molecule (such as, the specific antibody by coupling target substance on magnetic bead), therefore target substance can be collected rapidly.
Compare with method with existing separation equipment, immune magnetic separating device of the present utility model does not have particular/special requirement (such as to separation equipment, do not need chromatographic column), production process is few, not needing to optimize too much the separation condition of each production process when changing the target substance that will be separated, being therefore very suitable for suitability for industrialized production.The use of photoelectric position limit device simultaneously can ensure the to-and-fro movement of magnet, thus can improve Beads enrichment efficiency to a greater degree.
In sum, the utility model structure is simple, can be separated on a large scale rapidly (such as, pilot scale or industrial-scale are separated) to the specific objective material in blood plasma.
Accompanying drawing explanation
Fig. 1 is the right view of an embodiment of immune magnetic separating device of the present utility model;
Fig. 2 is the basic functional principle of immune magnetic separating device of the present utility model, shows two magnets 7 ' and 7 in figure " be arranged in separation container 18 both sides, contain magnetic bead 19 in separation container 18.
In the accompanying drawings, Reference numeral implication is as follows:
1 immune magnetic separating device
2 horizontal bases
3 columns
4 line slide rails
5 slide blocks
6 odontoid belts
7,7 ', 7 " magnet
8 magnet support arms
9 stepper motors and step-by-step controller
10 power supplys and logical circuit controller
11 up photoelectric position limit devices
12 descending photoelectric position limit devices
13 up safety position limiters
14 descending safety position limiters
15 odontoid belts and magnet support arm point of fixity
16 bracing frames
17 stepper motor driving wheels
18 separation containers
19 magnetic beads
20 solution to be separated
Embodiment
Fig. 1 schematically illustrates an embodiment of immune magnetic separating device of the present utility model.In FIG, immune magnetic separating device 1 comprises: horizontal base 2; Column 3; Line slide rail 4, slide block 5 and odontoid belt 6; Magnet 7 and magnet support arm 8; Stepper motor and step-by-step controller 9; Power supply and logical circuit controller 10; Up photoelectric position limit device 11 and descending photoelectric position limit device 12; Up safety position limiter 13 and descending safety position limiter 14; Odontoid belt and magnet support arm point of fixity 15; Bracing frame 16; Stepper motor driving wheel 17.Also separation container 18 is shown with dotted line in Fig. 1.Solution 20 to be separated and distribution magnetic bead 19 (see Fig. 2) is in the solution accommodated in separation container 18.
In the utility model, line slide rail 4 is vertically fixed on column 3, and slide block 5 can be lower mobile in the vertical direction on described line slide rail 4; Magnet 7 is fixed on the free-end of magnet support arm 8, and the other end of magnet support arm 8 to be fixed on slide block 5 and to be fixedly connected with odontoid belt 6; Stepper motor driving wheel 17 is connected with odontoid belt 6; And according to uplink and downlink photoelectric position limit device (11,12) signal sent, controllor for step-by-step motor 9 sends instruction control step motor 9, and (stepper motor and controllor for step-by-step motor show with an entirety in FIG, be labeled as 9) forward or reverse, thus control odontoid belt 6 drives magnet support arm 8 to move up and down on line slide rail 4.
Magnet 7 can be selected from electromagnet and permanent magnet.Permanent magnet can be natural magnet or artificial magnet, and the selection of artificial magnet is unrestricted, can use any artificial magnet as known in the art, such as alnico magnet, ferrite lattice, ndfeb magnet etc., the ndfeb magnet that preferred magnetic is the strongest.
In FIG, power unit and logical circuit controller entirety are with numeral 10 mark.Logical circuit controller can comprise the control assembly such as logical circuit and control knob (such as up button).
Fig. 2 schematically illustrates principle of work of the present utility model.In fig. 2, when immune magnetic separating device 1 of the present utility model is in standby condition, separation container 18 is placed on two magnets (7 '; 7 ") both sides, for the consideration to magnetic fields scope, distance between magnet (7 ', 7 ") and the chamber wall of separation container 18 is generally less than 0.5 centimetre, is preferably less than 0.1-0.3 centimetre.Containing solution 20 to be separated in separation container 18, inserted magnetic bead 19 in solution 20 to be separated, on magnetic bead 19, coupling has the antibody of combining target material.The distribution situation of (time at electromagnet for energising) magnetic line of force when magnet (7 ', 7 ") produces magnetic field is also schematically shown in Fig. 2.Under the influence of a magnetic field, a part of magnetic bead 19 in solution 20 to be separated will move respectively to two magnets (7 ', 7 ") and attracted to both sides on the chamber wall of magnet.When magnet (7 '; 7 ") when moving down along chamber wall, part magnetic bead 19 attracted on container side wall, other magnetic bead 19 is followed magnet and is moved down under the impact of the magnetic line of force, the resistance that the viscosity that can overcome high viscosity solution (such as blood plasma) declines to magnetic bead, thus accelerate magnetic bead settling velocity, and then improve separation efficiency.When magnet (7 '; 7 ") when moving to container bottom, magnet or power-off can be removed (at magnet (7 '; 7 ") for electromagnet when), the magnetic bead 19 be attracted to after magnetic field dissipate on container side wall is fallen bottom container 18 under gravity, now by any means easily, the whole magnetic beads being deposited to container bottom can be reclaimed and (such as, escape hole is set bottom container 18, utilize its discharge of Valve controlling; Or collected by other magnetic collection device; Etc.).Afterwards, magnet can be moved to container 18 top and repeat downward movement, magnetic bead residual in further separation container.Such downward movement can be repeatedly.
As can be seen here, immune magnetic separating device of the present utility model does not affect by the target substance that will be separated, only relevant with the density of magnetic bead in the viscosity of the size of separation container, solution to be separated, separation container etc.Those skilled in the art can determine the magnetic force size of magnet, slide block displacement and the parameter such as the distance between magnet and separation container and multiplicity according to the viscosity, bead density etc. of the size of separation container, solution to be separated.
In the utility model, the magnetic bead of any type for separating of biomolecule as known in the art can be used, unrestricted in the utility model.Described magnetic bead can coupling is special to target biological molecules according to procedures known in the art specific antibody.
Illustrate the operating process of the embodiment of the of the present utility model immune magnetic separating device shown in Fig. 1 below:
1. power supply energising, presses up button (be arranged in power supply and logic controller 10, do not show in figure);
2. stepper motor 9 works, and stepper motor driving wheel 17 drives odontoid belt 6 to rotate, and band movable slider 5 is up, now electromagnet 7 no power;
3. the up arrival of slide block 5 up photoelectric position limit device 11 place, triggers up photoelectric position limit device 11;
4. up photoelectric position limit device 11 starts, and control step motor 9 reverses, and stepper motor driving wheel 17 drives odontoid belt 6 to rotate, and band movable slider 5 is descending, and electromagnet 7 is energized simultaneously;
5. slide block 5 goes downwards to descending photoelectric position limit device 12 place, and triggers descending photoelectric position limit device 12;
6. descending photoelectric position limit device 12 starts, and namely stepper motor 9 rotates forward, and stepper motor driving wheel 17 drives odontoid belt 6 to rotate, and band movable slider 5 is up, simultaneously electromagnet 7 power-off (now magnetic line of force disappearance);
7. as needed repeated isolation, be then back to the 3rd step, or if do not needed further separation, then by power cut-off.
It should be noted that; in immune magnetic separating device in FIG; also be provided with up safety position limiter 13 and descending safety position limiter 14; both effects are when uplink and downlink photoelectric position limit device (11; 12) start time malfunctioning; make device power-off, protect whole tripping device and separation container 18.
Claims (7)
1. an immune magnetic separating device, is characterized in that described immune magnetic separating device comprises:
Horizontal base;
Column;
Elevating mechanism, comprises line slide rail, slide block and odontoid belt;
Magneto separate mechanism, comprises magnet and magnet support arm;
Driving mechanism, comprises stepper motor and photoelectric position limit device;
Power supply; With
Control gear, comprises logical circuit controller and controllor for step-by-step motor;
Wherein said line slide rail is vertically fixed on described column, and described slide block can be lower mobile in the vertical direction on described line slide rail;
Described magnet support arm comprises free-end and stiff end, and described magnet is fixed on the free-end of described magnet support arm, and the stiff end of described magnet support arm to be fixed on described slide block and to be fixedly connected with described odontoid belt;
The driving wheel of described stepper motor is connected with described odontoid belt; And
According to the signal that described photoelectric position limit device sends, controllor for step-by-step motor sends instruction control step motor forward or reverse, thus controls described odontoid belt and drive described magnet support arm to move up and down on described line slide rail.
2. immune magnetic separating device according to claim 1, is characterized in that described Magneto separate mechanism comprises two magnet support arms and two magnets, each magnet support arm is fixed a magnet.
3. immune magnetic separating device according to claim 1, it is characterized in that described photoelectric position limit device comprises uplink and downlink photoelectric position limit device, described uplink and downlink photoelectric position limit device is arranged on the upper and lower of described column respectively.
4. immune magnetic separating device according to claim 2, it is characterized in that the separation container of the solution held containing magnetic particle is placed between described two magnets, the distance when placing described separation container between described magnet and the chamber wall of described separation container is less than 0.5 centimetre.
5. immune magnetic separating device according to claim 4, is characterized in that described magnetic particle is magnetic bead.
6. the immune magnetic separating device according to any one of claim 1-5, is characterized in that described magnet is electromagnet.
7. the immune magnetic separating device according to any one of claim 1-5, is characterized in that described magnet is permanent magnet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520918181.6U CN205091342U (en) | 2015-11-17 | 2015-11-17 | Immunity magnetic separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520918181.6U CN205091342U (en) | 2015-11-17 | 2015-11-17 | Immunity magnetic separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205091342U true CN205091342U (en) | 2016-03-16 |
Family
ID=55482015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520918181.6U Active CN205091342U (en) | 2015-11-17 | 2015-11-17 | Immunity magnetic separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205091342U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108927285A (en) * | 2018-05-23 | 2018-12-04 | 齐齐哈尔医学院 | The micro- magnetic ball fast desorption device of nanometer |
| CN113484121A (en) * | 2021-06-28 | 2021-10-08 | 同济大学 | Separation and purification device and separation and purification method based on dynamic magnetic field |
-
2015
- 2015-11-17 CN CN201520918181.6U patent/CN205091342U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108927285A (en) * | 2018-05-23 | 2018-12-04 | 齐齐哈尔医学院 | The micro- magnetic ball fast desorption device of nanometer |
| CN108927285B (en) * | 2018-05-23 | 2020-03-24 | 齐齐哈尔医学院 | Nano micro magnetic ball quick desorption device |
| CN113484121A (en) * | 2021-06-28 | 2021-10-08 | 同济大学 | Separation and purification device and separation and purification method based on dynamic magnetic field |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Minghui Inventor after: Gao Hongqi Inventor after: Fu Lin Inventor before: Gao Hongqi Inventor before: Zhang Minghui Inventor before: Fu Lin |
|
| COR | Change of bibliographic data |