CN204905212U - A ion transport device for formation of image of biological tissue mass spectrum - Google Patents
A ion transport device for formation of image of biological tissue mass spectrum Download PDFInfo
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- CN204905212U CN204905212U CN201520580709.3U CN201520580709U CN204905212U CN 204905212 U CN204905212 U CN 204905212U CN 201520580709 U CN201520580709 U CN 201520580709U CN 204905212 U CN204905212 U CN 204905212U
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Abstract
The utility model provides an ion transport device for formation of image of biological tissue mass spectrum, the device includes electron spray nozzle, objective table and mass spectrograph collection awl, places sample in an organized way on the objective table, being equipped with the ion transport pipe and sealing up the sleeve pipe between electron spray nozzle and mass spectrograph collection awl, mass spectrograph collection awl one end is close to at the ion transport pipe to sealed sleeve pipe setting, adopt sealing connection between the one end of sealed sleeve pipe and the mass spectrograph collection awl, the air -extractor is installed to the other end, has the flowmeter at the internally mounted who seals up the sleeve pipe. The utility model discloses can be in more remote realization tissue the ionizationoun of molecule, the sensitivity of formation of image analysis and detection improves, enlarged the little molecular type's of endogenous coverage, can ally oneself with usefulness with current commercial mass spectrograph simultaneously.
Description
Technical field
The utility model belongs to the mass ions source technology of life science, field of medicaments, and particularly, the utility model relates to the ionogenic auxiliary equipment of a kind of biological tissue mass spectrum imaging.
Background technology
Mass spectrum imaging is a kind of novel Molecular imaging techniques based on mass spectral analysis, its the most outstanding feature be when exempt from mark, without the need to complex sample pre-treatment, by once testing the chemical information and spatial distribution characteristic that directly obtain the multiple biologically functional molecule in histotomy surface, and specific ion image can be associated with Histopathological Characteristics, therefore in the field such as clinical medicine, molecular biology, there is major application prospect.
Along with the progress and development of science and technology, study relevant technology to biological tissue and means increasing.Optical image technology under traditional microscope can observe tissue and cellular morphology, but can not provide any relevant biochemical information; Though conventional Measurement for Biochemistry can obtain a certain amount of biochemical information, can not the spatial distribution characteristic of characterizing biomolecules.Mass spectrum imaging is a kind of the highly sensitive of mass-spectrometric technique and high specific, the original position of surface area technology and visual novel molecular imaging technique of image processing techniques of combining.The tissue physiology that the molecular species obtained by this technology, relative abundance and distribution phasor can be observed with conventional microscope or pathological characters are associated, and therefore in life science and medical research, have become a kind of strong analysis tool.Matrix assisted laser desorption ionization mass spectrum imaging (MALDI-MSI) and parsing electrospray ionization mass spectrum imaging (DESI-MSI) directly can obtain protein, polypeptide, lipid, medicine and other endogenous metabolism thing distribution situation in the tissue.MALDI-MSI need operate in a vacuum, and convenience is poor; And need to cover matrix absorption laser energy on tissue sections and realize ionization, and the low quality region in mass spectrogram produces a large amount of matrix peaks, covers and disturbs micromolecular Mass Spectrometer Method.Current DESI-MSI mainly can realize the imaging analysis of lipid compound in tissue, be that content is high in the tissue because of lipid, and mass ions responds.There is a difficult problem to the micromolecular imaging analysis of endogenous in tissue in therefore current mass spectrum imaging technology, needs the coverage improving the micromolecular detection sensitivity of endogenous in organizing and molecular species; On the other hand, still there is larger difficulty under comparatively at a distance, realize biological tissue's Middle molecule imaging analysis or Mass Spectrometer Method.
Utility model content
The purpose of this utility model is to provide a kind of ion transport device for biological tissue's mass spectrum imaging, endogenous Small molecular in biological tissue can be delivered to the collection cone of the equipment such as mass spectrometer with descending maximum more at a distance, realize highly effective gathering, and then improve the sensitivity of imaging analysis and detection.
The technical solution of the utility model is as follows:
A kind of ion transport device for biological tissue's mass spectrum imaging, this device comprises electron spray nozzle, objective table and mass spectrometer collection cone, objective table is placed with tissue sample, it is characterized in that: between electron spray nozzle and mass spectrometer collection cone, be provided with ion transfer tube and Sealed casing pipe; Sealed casing pipe is arranged on ion transfer tube and bores one end near mass spectrometer collection; One end of Sealed casing pipe and mass spectrometer collection adopt between boring and are tightly connected, and the other end is provided with air-extractor, between Sealed casing pipe and air-extractor, be provided with flowmeter; The axis angle that the axis of ion transfer tube and mass spectrometer collection are bored is 0 ~ 90 degree.
As a kind of preferred version, ion transfer tube outlet and mass spectrometer collection bore between be apart 0-20 millimeter.Ion transfer bore is 0.1 ~ 50 millimeter.
As a kind of preferred version, the port shape of ion transfer tube near one end of tissue sample is cylindrical shape, taper barrel or wedge shape, and wedge shape corner cut becomes 0 ~ 60 ° with ion transfer tube axis.
As a kind of preferred version, the distance between both electron spray nozzle and ion transfer tube is at 0 ~ 10 millimeter.
As a kind of preferred version, the angle of ion transfer tube axis and tissue sample place plane is 0 ~ 90 ° of scope.
As a kind of preferred version, described air-extractor adopts vacuum pump or air compressor.
The utility model achieves biological tissue's ionization under atmospheric conditions, and can obtain highly sensitive detection under remote condition.Compared with existing ion source transmission means, have the following advantages and effect: the 1. air-extractor of low vacuum: produce certain negative pressure in the perimeter of the acquisition interface of mass spectrometer plasma checkout equipment, pressure is less than atmospheric pressure, but be greater than its interior zone, to there is provided in ion transfer tube required air-flow, flow is greater than 0 liter/min and 0-100 liter/min of adjustment.Adopt the mode that this active sucks, reduce the ionogenic effects that dispel of open type such as DESI, improve collecting efficiency, be delivered to maximum the collection cone of the equipment such as mass spectrometer, thus improve sensitivity to a great extent.2. the air of high flow capacity, high speed is as the motion carrier of charged particle, can reduce the loss of ion in transmitting procedure, realize ion long-distance transmissions under atmospheric conditions.3. the air of high flow capacity, high speed is as the motion carrier of charged particle, can promote the desolvated degree of charged drop in ionization process, promotes sample ionization.4. based on principle of inertia and employing air back suction, the particles such as the air of little quality, solvent can be separated, realize charged particle in checkout equipment acquisition interface enrichments such as mass spectrometers.5. expand space and the flexibility of sample, increase the analysis ability to bulky items, will the application of imaging of tissue be expanded, as the real-time Detection and diagnosis in clinical operation.6. because improve the sensitivity of open type mass spectrum imaging to a great extent, especially expanding the coverage of endogenous Small molecular type, the mass spectrum imaging analysis of the micromolecular high-quality biological tissue of polymorphic type low content endogenous can be realized.
Accompanying drawing explanation
The structural representation of a kind of ion transport device for biological tissue's mass spectrum imaging that Fig. 1 provides for this experiment is novel.
Fig. 2 is the influence curve of gas flow rate to biological tissue's (ionic strength) detection sensitivity.
In figure: 1-electron spray nozzle; 2-tissue sample; 3-objective table; 4-ion transfer tube; 5-Sealed casing pipe; The collection of 6-mass spectrometer is bored; 7-flowmeter; 8-air-extractor.
Embodiment
In order to make the purpose of this utility model, technical scheme and technique effect clearly, below in conjunction with drawings and Examples, the utility model will be further described.
The structural representation of a kind of ion transport device for biological tissue's mass spectrum imaging that Fig. 1 provides for the utility model, this transmitting device comprises electron spray nozzle 1, objective table 3, mass spectrometer collection cone 6, transfer tube 4 and Sealed casing pipe 5; Objective table 3 is placed with tissue sample 2, and ion transfer tube 4 and Sealed casing pipe 5 are arranged between electron spray nozzle 1 and mass spectrometer collection cone 6; Sealed casing pipe 5 is arranged on one end that ion transfer tube 4 is bored near mass spectrometer collection; One end of Sealed casing pipe 5 and mass spectrometer collection adopt between boring 6 and are tightly connected, and the other end is provided with air-extractor 8, and described air-extractor 8 can adopt vacuum pump or air compressor.Flowmeter 7 is arranged on the inside of Sealed casing pipe 5.
The axis angle that the axis of ion transfer tube 4 and mass spectrometer collection bore 6 is 0 ~ 90 degree, and as a kind of preferred version, the axis angle that the axis of ion transfer tube and mass spectrometer collection are bored is 60 ~ 70 degree.Ion transfer tube outlet and mass spectrometer collection bore between be apart 0-20 millimeter.The internal diameter of ion transfer tube is generally 0.1 ~ 50 millimeter.The port shape of ion transfer tube near one end of tissue sample is cylindrical shape, taper barrel or wedge shape, and wedge shape corner cut becomes 0 ~ 60 ° with the axis of ion transfer tube, is preferably 15 degree.Distance between both electron spray nozzle and ion transfer tube is at 0 ~ 10 millimeter.The axis of ion transfer tube and the angle of tissue sample 2 place plane are 0 ~ 90 degree of scope, and being preferably angle is 15 degree.
The course of work of the present utility model is as follows:
Biological tissue is made into frozen section, and tissue sample 2 is fixed on objective table 3 or under its primal environment, by control device, by automatic path planning, mass spectrum imaging scanning is carried out to appointment or whole tissue regions; Ion transfer tube 4 applies certain voltage, to make it and mass spectrometer collection is bored between 6 and formed certain electrical potential difference; Use air-extractor in ion transfer tube, produce the air-flow of certain speed, its direction is that tissue sample 2 region bores 6 to mass spectrometer collection, and flowmeter 7 controls and monitors flow velocity size, and air velocity is generally about 60 liters/min; The front end of ion transfer tube and tissue sample close to and resolves some positions with tissue and keeps invariable, the secondary charged drop containing Middle molecule in a organized way brings ion transfer tube in a large number through air-flow, and realizes desolventizing and further ionization in the process.
When the axis angle that the axis of ion transfer tube and mass spectrometer collection bore 6 is arranged with acute angle, the pollution to mass spectrometer collection cone 6 such as solid, neutral drop can be reduced, improve durability and the stability of instrument.
In the front end of ion transfer tube, the initial charged particles produced by certain mode acts on tissue sample surface, and carries out parsing formation secondary charged drop or ion to tissue sample, brings ion transfer tube in large quantities through moving air, realize highly effective gathering, and accelerated.Be delivered to mass spectrometer collection cone by the effect of air-flow simultaneously, finally enter mass spectrometer and detected, obtain the Information in Mass Spectra of tissue sample Middle molecule.Carry out processing and reconstruct to data eventually through mass spectrum imaging software, obtain the mass spectrum imaging figure organized.
Fig. 2 is the impact of gas with various flow velocity on biological tissue's detection sensitivity.Can find out from the change of figure medium sensitivity and can obtain higher detection sensitivity when gas flow rate in transfer tube is 60 liters/min.
Claims (7)
1. the ion transport device for biological tissue's mass spectrum imaging, this device comprises electron spray nozzle (1), objective table (3) and mass spectrometer collection cone (6), objective table (3) is placed with tissue sample (2), it is characterized in that: between electron spray nozzle (1) and mass spectrometer collection cone (6), be provided with ion transfer tube (4) and Sealed casing pipe (5); Sealed casing pipe (5) is arranged on ion transfer tube (4) near mass spectrometer collection cone (6) one end; One end of Sealed casing pipe (5) and mass spectrometer collection are bored to adopt between (6) and are tightly connected, the other end is provided with air-extractor (8), is provided with flowmeter (7) between Sealed casing pipe (5) and air-extractor (8); The axis angle that (6) are bored in the axis of ion transfer tube (4) and mass spectrometer collection is 0 ~ 90 degree.
2. according to a kind of ion transport device for biological tissue's mass spectrum imaging according to claim 1, it is characterized in that: it between (6) is apart 0-20 millimeter that ion transfer tube (4) outlet and mass spectrometer collection bores.
3. according to a kind of ion transport device for biological tissue's mass spectrum imaging according to claim 1, it is characterized in that: ion transfer tube (4) internal diameter is 0.1 ~ 50 millimeter.
4. according to a kind of ion transport device for biological tissue's mass spectrum imaging described in claim 1,2 or 3, it is characterized in that: the port shape of ion transfer tube (4) near one end of tissue sample (2) is cylindrical shape, taper barrel or wedge shape, and wedge shape corner cut becomes 0 ~ 60 ° with ion transfer tube (4) axis.
5. according to a kind of ion transport device for biological tissue's mass spectrum imaging described in claim 1,2 or 3, it is characterized in that: electron spray nozzle (1) and ion transfer tube (4) distance between the two is at 0 ~ 10 millimeter.
6. according to a kind of ion transport device for biological tissue's mass spectrum imaging described in claim 1,2 or 3, it is characterized in that: the angle of ion transfer tube (4) axis and tissue sample (2) place plane is 0 ~ 90 ° of scope.
7. according to a kind of ion transport device for biological tissue's mass spectrum imaging according to claim 1, it is characterized in that: described air-extractor (8) adopts vacuum pump or air compressor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201520580709.3U CN204905212U (en) | 2015-08-04 | 2015-08-04 | A ion transport device for formation of image of biological tissue mass spectrum |
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| CN201520580709.3U CN204905212U (en) | 2015-08-04 | 2015-08-04 | A ion transport device for formation of image of biological tissue mass spectrum |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105973973A (en) * | 2016-07-11 | 2016-09-28 | 华南师范大学 | Biological tissue mass spectrometry imaging method |
| CN110044997A (en) * | 2018-01-15 | 2019-07-23 | 中国医学科学院药物研究所 | A kind of ionic strength of internal drug virtually corrects and quantitative mass spectral i mage analysis methods |
| CN111540665A (en) * | 2020-04-20 | 2020-08-14 | 清华大学深圳国际研究生院 | Ionization device and application thereof |
-
2015
- 2015-08-04 CN CN201520580709.3U patent/CN204905212U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105973973A (en) * | 2016-07-11 | 2016-09-28 | 华南师范大学 | Biological tissue mass spectrometry imaging method |
| CN105973973B (en) * | 2016-07-11 | 2020-10-20 | 华南师范大学 | Biological tissue mass spectrum imaging method |
| CN110044997A (en) * | 2018-01-15 | 2019-07-23 | 中国医学科学院药物研究所 | A kind of ionic strength of internal drug virtually corrects and quantitative mass spectral i mage analysis methods |
| CN110044997B (en) * | 2018-01-15 | 2023-08-04 | 中国医学科学院药物研究所 | Ion intensity virtual correction and quantitative mass spectrum imaging analysis method for in-vivo medicine |
| CN111540665A (en) * | 2020-04-20 | 2020-08-14 | 清华大学深圳国际研究生院 | Ionization device and application thereof |
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