CN204067299U - A kind of enclosed type molecule adduction isotope ion generator - Google Patents
A kind of enclosed type molecule adduction isotope ion generator Download PDFInfo
- Publication number
- CN204067299U CN204067299U CN201420313741.0U CN201420313741U CN204067299U CN 204067299 U CN204067299 U CN 204067299U CN 201420313741 U CN201420313741 U CN 201420313741U CN 204067299 U CN204067299 U CN 204067299U
- Authority
- CN
- China
- Prior art keywords
- isotope
- capillary
- ion
- container
- taylor cone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 230000005611 electricity Effects 0.000 claims abstract description 12
- 230000000155 isotopic effect Effects 0.000 claims abstract description 8
- 230000008676 import Effects 0.000 claims description 6
- 239000011800 void material Substances 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 150000002500 ions Chemical class 0.000 description 49
- 239000007789 gas Substances 0.000 description 22
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 12
- 239000007921 spray Substances 0.000 description 12
- 230000005684 electric field Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000001793 charged compounds Chemical class 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003595 mist Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000003570 air Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The utility model discloses a kind of ion generating device, specifically refer to a kind of enclosed type generator for generation of molecule adduction isotope ion.The utility model comprises a taylor cone generator, has the container (4) of a closed placement isotope gas or liquid vapour in capillary (2) exit of taylor cone generator (1); Taylor cone generator (1) comprises capillary (2), be connected to high-tension electricity joint in the outside of capillary (2), high-tension electricity electric connection forms a circuit loop with container (4) inwall of the placement isotope gas closed or liquid vapour.The utility model has the advantages that the present situation that effectively can change and need a large amount of isotopic source just can produce enough molecule adduction isotope ion bundle groups in the past, make applying of isotope ion become possibility, greatly reduce isotope ion production cost; And use this device convenient.Because isotope ion and molecule adduct ion bundle group have the industries such as biology, medicine, food to have purposes widely.
Description
Technical field
The utility model relates to a kind of ion generating device, specifically refers to a kind of enclosed type generator for generation of molecule adduction isotope ion.
Background technology
Since the mid-80, electric spray ion source has been applied to since large molecular mass analyzes by one of Nobel chemistry Prize winner in 2002 John B.Fenn, past more than 20 year, for electric spray ion source mechanism, still two patterns are rested on: Ion Evaporation Model (IEM) ion evaporation, with Charged Residue Model (CRM) charge residue mechanism.The basic structure of electric spray ion source and the difference do not had in essence of the nineties.
The basic structure of electric spray ion source is exactly be full of liquid in the middle of the metal or capillary glass tube of a hollow, facing to mass spectrometric ion entrance, liquid adds plus or minus high voltage, form negative ions in an atmosphere, a part of ion is sucked mass spectrometric mass analyzer by mass spectrometric vacuum system.
For electric spray ion source, although ionization probability is very high, almost reach 100%, molecular ion be transferred to the effective ion of mass spectrum mass detector, only have total number of ions 0.01% to 0.1% between.
In order to solve the problem, a lot of inventor has invented the electric spray ion source of different institutions, and referenced patent number is: the U.S. US4861988, US5412208, US5432343, US6992299, US5504329.But all inventions are all be among air by the transmitting needle point of electric spray ion source, ion current and fluid flow in close relations, 100 cannot to receive liter/min within the scope of large discharge ~ 100 microlitres/point obtain stable emission of ions, and ion transmission efficiency is not significantly improved.
For the formation of taylor cone, " computer and applied chemistry " the 28th volume o. 11th have corresponding open explanation, wherein by control voltage size, solution is made to form drop all the time at the capillary mouth of pipe and not drip, at this moment, the drop of capillary lower end is that " solution is subject to the effect of surface tension and electric field force at the capillary mouth of pipe to the hemispherical of convex simultaneously, along with electric field strength increases, same sex electric charge in solution is gathered in drop surface, the electric field that surface charge produces causes drop deformation " when voltage reaches a certain critical value Vc, the solution at mouth of pipe place gradually becomes taper by hemisphere, during balance, its angle, half top is approximately 493 ", this charged cone is called taylor cone (Tay lor Cone) by everybody ".
Electric spray ion source (ESI) is the most frequently used interface of current liquid chromatography-mass spectrography (LC-MS) coupling, belongs to soft ionization mode, can be used for studying thermally labile and the larger compound of polarity.Due to can the molecular ion of generating strap multi-charge, electric spray ion source also can be used for the large biological molecules such as Study on Protein.The first provings such as Fenn ESI is generating the applicability in biomolecular ions, until the sixties in 20th century, Dole etc. produce the mass spectrometer of First based on electron spray.Afterwards, Fenn etc. use ESI series connection level Four bar detector to detect molecular beam, recognize Dole ion source existing defects, and make an explanation to result, have modified the distance between ion source nozzle needle and end plate, thus reduce nozzle needle voltage.Ion source designed by Fenn developed into Fenn-Whitehouse design afterwards.This design uses capillary glass tube that ion is delivered into the first vacuum chamber from atmospheric environment, can select different inner diameters capillary according to demand.1987, Aeroassisted sprayer was introduced electrospray interface by Bruins etc.Atomization gas contributes to stable electrical spraying, keeps flow velocity close to 0.2mL/min, is applicable to liquid phase series connection ESI-MS. and allows nozzle and to there being larger distance between electrode, decrease the generation of corona discharge.Meanwhile, reduce the dependence of spray process to nozzle location, if place nozzle from axle, higher sensitivity can be obtained.After this, electric spray ion source structural design have passed through a series of improvement, there has also been more and more deep understanding to its operation principle.
At present, technical staff emphasizes at taylor cone generator (in this application always, the device forming taylor cone phenomenon is referred to as taylor cone generator) in liquid in capillary be produce ion basic, and always unknown for phenomenons the such as whether liquid at capillary outlet place can be ionized.In current demand, in order to obtain isotopic ionization, the effect formed by taylor cone of the prior art, causes needing in isotope ion process very highly have cost, so the application for isotope ion is subject to suitable restriction.
Utility model content
The utility model, for deficiency of the prior art, provides a kind of device conveniently can preparing molecule adduction isotope ion, can become possibility for the extensive use of isotope ion.
The utility model is achieved by following technical proposals:
A kind of enclosed type molecule adduction isotope ion generator, is characterized in that, a taylor cone generator, has the container of a closed placement isotope gas or liquid vapour at the capillary outlet place of taylor cone generator.
As preferably, in above-mentioned a kind of enclosed type molecule adduction isotope ion generator, taylor cone generator comprises capillary, be connected to high-tension electricity joint in the outside of capillary, high-tension electricity electric connection forms a circuit loop with the container inner wall of the placement isotope gas closed or liquid vapour.In order to realize better effect, between capillary and container inner wall, have a gas flow void layer, gas flow void layer is in communication with the outside by several side openings.Body of ventilating be the mixing of several gas in nitrogen, oxygen, argon gas, hydrogen, air or above-mentioned gas, the nitrogen in above-mentioned gas or air have better effect.
As preferably, the placement isotope gas closed in above-mentioned a kind of enclosed type molecule adduction isotope ion generator or the container of liquid have an opening to be connected with mass spectrometer, and the import of taylor cone generator is connected with polar liquid; Guarantee in capillary, have continuous print taylor cone phenomenon to exist.
As preferably, in above-mentioned a kind of enclosed type molecule adduction isotope ion generator, the capillary inlet of taylor cone generator is connected with chromatograph.
As preferably, the placement isotope gas closed in above-mentioned a kind of enclosed type molecule adduction isotope ion generator or the container of liquid vapour are connected with isotopic source.
As preferably, above-mentioned a kind of enclosed type molecule adduction isotope ion generator, has valve between the container that it is characterized in that isotopic source and isotope gas or liquid vapour; Effectively can regulate the flow of isotope gas or liquid vapour like this, the handled easily such as to control cost.
Beneficial effect: the utility model effectively can change the present situation in the past needing a large amount of isotopic source just can produce enough molecule adduction isotope ion bundle groups, makes applying of isotope ion become possibility, greatly reduces isotope ion production cost; And use this device convenient.Because isotope ion and molecule adduct ion bundle group have the industries such as biology, medicine, food to have purposes widely, therefore this extensive use can be realized by the utility model more fully.
Accompanying drawing explanation
Fig. 1 structural representation of the present utility model
The analysis of spectra of air near taylor cone in Fig. 2 the utility model embodiment
Air and heavy water D near taylor cone in Fig. 3 the utility model embodiment
2the analysis of spectra of O mist
Embodiment
Below enforcement of the present utility model is illustrated:
Embodiment 1
Structure with reference to the accompanying drawings shown in 1, makes an enclosed type molecule adduction isotope ion generator, comprising a taylor cone generator, has the container 4 of a closed placement isotope gas or liquid vapour in capillary 2 exit of taylor cone generator 1; Be connected to high-tension electricity joint in the outside of capillary 2, high-tension electricity electric connection forms a circuit loop with container 4 inwall of the placement isotope gas closed or liquid vapour.
Be connected with polar liquid water in the import of taylor cone generator 1, then under the effect of high-tension electricity, polar liquid, by electric field action, forms an electric field between the outlet and the inwall of container 4 of capillary 2, has isotope heavy water (D in container 4 simultaneously
2o), at the exit heavy water (D of capillary 2
2o) ion is formed by electric field action.
By analyzing produced ion, measuring near taylor cone, in order to obtain good contrast effect, first the air near taylor cone being measured, gained collection of illustrative plates as shown in Figure 2, then near taylor cone being air and heavy water D
2o mist measures, as shown in Figure 3 collection of illustrative plates.
Embodiment 2
Structure with reference to the accompanying drawings shown in 1, makes an enclosed type molecule adduction isotope ion generator, comprising a taylor cone generator, has the container 4 of a closed placement isotope gas or liquid vapour in capillary 2 exit of taylor cone generator 1; Be connected to high-tension electricity joint in the outside of capillary 2, high-tension electricity electric connection forms a circuit loop with container 4 inwall of the placement isotope gas closed or liquid vapour.
Be connected with polar liquid water in the import of taylor cone generator 1, then under the effect of high-tension electricity, polar liquid, by electric field action, forms an electric field between the outlet and the inwall of container 4 of capillary 2, has isotope heavy water (D in container 4 simultaneously
2o), at the exit heavy water (D of capillary 2
2o) ion is formed by electric field action.And a gas flow void layer is had between capillary 2 and container 4 inwall, gas flow void layer is in communication with the outside by several side openings, can pass into N
2as assist gas.Then an opening is had to be connected with mass spectrometer 3 at the container 4 of the placement isotope gas closed or liquid, the import connection of taylor cone generator 1 and chromatograph; Valve 5 is had, the control of more convenient like this dose of an isotope between isotopic source 6 and the container 4 of isotope gas or liquid vapour.
Carry out data to the present embodiment to record, also can obtain effect as shown in Figure 2 and Figure 3.
Claims (6)
1. an enclosed type molecule adduction isotope ion generator, it is characterized in that, comprise a taylor cone generator, have the container (4) of a closed placement isotope gas or liquid vapour in capillary (2) exit of taylor cone generator (1); Taylor cone generator (1) comprises capillary (2), be connected to high-tension electricity joint in the outside of capillary (2), high-tension electricity electric connection forms a circuit loop with container (4) inwall of the placement isotope gas closed or liquid vapour.
2. a kind of enclosed type molecule adduction isotope ion generator according to claim 1, it is characterized in that there is a gas flow void layer between capillary (2) and container (4) inwall, gas flow void layer is in communication with the outside by several side openings.
3. a kind of enclosed type molecule adduction isotope ion generator according to claim 1, it is characterized in that the container (4) of placement isotope gas or the liquid closed has an opening to be connected with mass spectrometer (3), the import of taylor cone generator (1) is connected with polar liquid.
4. a kind of enclosed type molecule adduction isotope ion generator according to claim 1, is characterized in that capillary (2) import of taylor cone generator (1) is connected with chromatograph.
5. a kind of enclosed type molecule adduction isotope ion generator according to claim 1, is characterized in that the container (4) of placement isotope gas or the liquid vapour closed is connected with isotopic source (6).
6. a kind of enclosed type molecule adduction isotope ion generator according to claim 1, has valve (5) between the container (4) that it is characterized in that isotopic source (6) and isotope gas or liquid vapour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420313741.0U CN204067299U (en) | 2014-08-21 | 2014-08-21 | A kind of enclosed type molecule adduction isotope ion generator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420313741.0U CN204067299U (en) | 2014-08-21 | 2014-08-21 | A kind of enclosed type molecule adduction isotope ion generator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204067299U true CN204067299U (en) | 2014-12-31 |
Family
ID=52208756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420313741.0U Expired - Fee Related CN204067299U (en) | 2014-08-21 | 2014-08-21 | A kind of enclosed type molecule adduction isotope ion generator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204067299U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104201084A (en) * | 2014-06-12 | 2014-12-10 | 浙江好创生物技术有限公司 | Closed type molecular adduct isotope ion generator |
-
2014
- 2014-08-21 CN CN201420313741.0U patent/CN204067299U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104201084A (en) * | 2014-06-12 | 2014-12-10 | 浙江好创生物技术有限公司 | Closed type molecular adduct isotope ion generator |
| CN104201084B (en) * | 2014-06-12 | 2017-02-01 | 浙江好创生物技术有限公司 | Closed type molecular adduct isotope ion generator |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6588118B2 (en) | Synchronizing ion production with the discontinuous atmospheric interface period | |
| US11631577B2 (en) | Ion focusing | |
| US9484195B2 (en) | Systems and methods for transfer of ions for analysis | |
| CN102709147B (en) | Electrospray ion source and mass spectrometer | |
| US6906322B2 (en) | Charged particle source with droplet control for mass spectrometry | |
| CN102142352B (en) | Electro-spray ion generator | |
| CN104241077B (en) | Normal pressure micro-glow discharge maldi mass spectrometer ion gun of magnetically confined and mass spectrometer | |
| CN106876241A (en) | Ultrasonic atomizatio Atomospheric pressure glow discharge ionization device | |
| CN109887825A (en) | A kind of chemical reaction intermediate Mass Spectrometer Method device and method | |
| CN208336147U (en) | The plasma ionization device of Atmospheric particulates | |
| CN204067299U (en) | A kind of enclosed type molecule adduction isotope ion generator | |
| CN201975366U (en) | Electro spray ion generator | |
| CN204228671U (en) | A kind of opening molecule adduction isotope ion generator | |
| CN104201084B (en) | Closed type molecular adduct isotope ion generator | |
| CN104037047B (en) | A kind of method preparing molecule adduction isotope ion | |
| CN209544279U (en) | A kind of chemical reaction intermediate Mass Spectrometer Method device | |
| CN104198624B (en) | A kind of opening molecule adduction isotope ion generator | |
| CN209247687U (en) | A kind of organic matter detection device of double focusing type fly chemical ionization mass spectrometry | |
| CN109860015B (en) | Composite ionization source device | |
| CN217544538U (en) | Secondary electrospray ionization source device, gas sample introduction system and reagent atomizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Closed type molecular adduct isotope ion generator Effective date of registration: 20160630 Granted publication date: 20141231 Pledgee: Yuhang Hangzhou science and technology Company limited by guarantee Pledgor: ZHEJIANG HAOCHUANG BIOTECH Co.,Ltd. Registration number: 2016330000039 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20170414 Granted publication date: 20141231 Pledgee: Yuhang Hangzhou science and technology Company limited by guarantee Pledgor: ZHEJIANG HAOCHUANG BIOTECH Co.,Ltd. Registration number: 2016330000039 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141231 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |