CN105973973B - Biological tissue mass spectrum imaging method - Google Patents
Biological tissue mass spectrum imaging method Download PDFInfo
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- CN105973973B CN105973973B CN201610547602.8A CN201610547602A CN105973973B CN 105973973 B CN105973973 B CN 105973973B CN 201610547602 A CN201610547602 A CN 201610547602A CN 105973973 B CN105973973 B CN 105973973B
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/626—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas
- G01N27/628—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas and a beam of energy, e.g. laser enhanced ionisation
Abstract
The invention discloses a biological tissue mass spectrum imaging method, which comprises the following steps: (1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices; (2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally air-drying; (3) dropwise adding acriflavine solution to the mouse kidney tissue slice, and standing to uniformly diffuse; (4) and (3) placing the corundum rod adhered with the mouse kidney tissue section dropwise added with the acriflavine solution in a mass spectrometer with laser for detection, and processing mass spectrum data ionized after laser analysis by software to obtain a mass spectrum imaging graph of the mouse kidney tissue containing acriflavine. The method is rapid and direct, matrix interference can be avoided, no complex sample pretreatment process exists, and the ionization imaging technology after laser analysis can adjust mass spectrum signals in time and space, thereby being beneficial to optimizing a mass spectrum imaging graph.
Description
Technical Field
The invention belongs to the technical field of mass spectrometry imaging, and particularly relates to a biological tissue mass spectrometry imaging method.
Background
The ionization mass spectrometry method after laser analysis has been greatly developed in the last twenty years, and compared with the traditional MALDI mass spectrometry method, the ionization mass spectrometry method has the advantages of rapidness, direct simplicity, no need of complex sample pretreatment and the like, so that the application of the ionization mass spectrometry method also slowly appears in the biological field, including the detection of biological micromolecules such as short peptides, amino acids and the like, drug molecules, chemical additives and other chemical molecules.
Mass spectrometry is a novel analytical technique for studying the distribution of molecules in biological cells, tissues and live animals, and can detect and record a spatial distribution of molecules in a particular system without labeling. The mass spectrometry technology was first appeared in 1997 and applied to the study of protein distribution in biological tissues, and with the rapid development of the mass spectrometry technology, the technology has been widely applied to the fields of proteome, lipidosome, drug metabolism and the like. Biological tissue imaging, as a general application of mass spectrometry, plays an important role in clinical medicine and disease diagnosis, and is accompanied by continuous innovation of mass spectrometry imaging technologies, and the traditional mass spectrometry imaging technologies including MALDI imaging, DESI imaging and the like have great limitations.
Conventional mass spectrometry imaging has the following disadvantages: (1) the speed is slow, and direct imaging cannot be realized; (2) the matrix interference is serious and the sample pretreatment is complicated; (3) the ionization imaging technology after laser analysis cannot adjust mass spectrum signals in time and space, and is not beneficial to optimizing a mass spectrum imaging graph.
Disclosure of Invention
The invention aims to provide a biological tissue mass spectrum imaging method which is rapid and direct, can avoid matrix interference and has no complex sample pretreatment process, and the ionization imaging technology after laser analysis can adjust mass spectrum signals in time and space, thereby being beneficial to optimizing a mass spectrum imaging graph.
The above object of the present invention is achieved by the following technical solutions: a method of mass spectrometry imaging of biological tissue comprising the steps of:
(1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices;
(2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally air-drying;
(3) dropwise adding acriflavine solution to the mouse kidney tissue slice, and standing to uniformly diffuse;
(4) and (3) placing the corundum rod adhered with the mouse kidney tissue section dropwise added with the acriflavine solution in a mass spectrometer with laser for detection, and processing mass spectrum data ionized after laser analysis by software to obtain a mass spectrum imaging graph of the mouse kidney tissue containing acriflavine.
In the above biological tissue mass spectrometry imaging method:
and (2) selecting Balb/c male mice in the step (1), and aging for 7-8 weeks.
And (3) cleaning the corundum rod in the step (2), adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally air-drying.
The concentration of the acriflavine solution in the step (3) is 10-3g/mL。
And (4) standing for 15-25 min in the step (3) to enable the dispersion to be uniform.
And (4) processing the ionized mass spectrum data after laser analysis in the step (4) by matlab software to obtain a mass spectrum imaging graph of the mouse kidney tissue containing acriflavine.
Compared with the prior art, the invention has the following advantages:
(1) the biological tissue mass spectrum imaging method has the advantages of no complex sample pretreatment and the like, effectively avoids the generation of experimental errors, reduces the complexity of experiments, and greatly improves the accuracy of the experiments;
(2) by adopting the method of the invention, the method takes the corundum rod as an experimental substrate and a novel biological tissue imaging technology, and the novel mass spectrometry imaging technology is used for imaging the mouse tissue injected with acriflavine intravenously to obtain the dynamic distribution of the drug in the mouse kidney, which can provide a good foundation for the development of future clinical medicine and the diagnosis of organ diseases such as kidney and the like;
(3) compared with the traditional mass spectrometry imaging method, the method has the advantages of milder detection conditions, simpler operation, quicker detection process and no interference of matrix, so the detection result is accurate, the sensitivity is high, the distribution of the medicine in the biological tissue can be detected and analyzed, and the novel biological tissue imaging method has wide application prospect in the fields of clinical medicine, kidney and other organ disease diagnosis;
(4) the method for directly researching the space distribution condition of the drug molecules on the mouse kidney by the laser desorption ionization mass spectrometry imaging technology provided by the invention saves the time for searching a proper matrix and avoids the influence of the matrix on the drug molecule signals in the experimental process, which shows that the imaging method can completely replace the traditional mass spectrometry imaging method to be applied to various fields as a novel biological mass spectrometry imaging technology.
Drawings
FIG. 1 is a mass spectrum obtained by using a laser-resolved ionization time-of-flight mass spectrometer to detect a pure drug molecule acriflavine by using a corundum rod as a matrix in example 1;
FIG. 2 is a mass spectrum obtained by using a laser-resolved ionization time-of-flight mass spectrometer to detect a pure drug molecule acriflavine by using a graphite rod as a matrix in example 1;
FIG. 3 is a mass spectrum obtained by detecting a mouse kidney tissue section with a corundum rod as a matrix by using a laser-resolved ionization time-of-flight mass spectrometer in example 2;
fig. 4 is an imaging diagram of kidney tissues to which acriflavine is added by using an ionization time-of-flight mass spectrometer after laser resolution, wherein the left image is a mouse kidney tissue section diagram, and the right image is a corresponding mass spectrum imaging diagram.
Detailed Description
Example 1
The mass spectrometry imaging method for the biological tissue provided by the embodiment comprises the following steps:
(1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices;
(2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally drying;
(3) dropwise adding acriflavine solution to the mouse kidney tissue slice, and standing to uniformly diffuse;
(4) and (3) fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer, and entering the mass spectrometer with laser through a sample injection system for detection to obtain a mass spectrogram of acriflavine in the mouse kidney tissue.
And (2) selecting Balb/c male mice in the step (1), and aging for 7-8 weeks.
The concentration of the acriflavine solution in the step (3) is 10-3g/mL，10-3When preparing g/mL acriflavine solution, ethanol is adopted as solvent, and the preparation concentration is 10-3g/mL acriflavine solution.
And (4) standing for 15-25 min in the step (3) to enable the dispersion to be uniform.
Fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer in the step (4), and entering the mass spectrometer with laser through a sample injection system for detection, wherein the specific detection process comprises the following steps: starting a mass spectrometer and a three-dimensional platform sample injector, respectively and sequentially emitting pulse lasers by two solid lasers in an ionization mass spectrometer after laser resolution, wherein a first beam of emitted pulse lasers is resolution lasers (infrared resolution lasers), the resolution lasers enter an ionization chamber after being focused and irradiate a mouse kidney tissue slice on a corundum rod, a second beam of pulse lasers is emitted after being delayed for 18-23 mu s, the second beam of pulse lasers is converted into vacuum ultraviolet lasers after being subjected to frequency tripling through a gas pool, the second beam of pulse lasers and the vacuum ultraviolet lasers are focused and separated by a flat convex mirror at a light outlet of the gas pool, the vacuum ultraviolet lasers enter the ionization chamber in a direction parallel to the corundum rod after being focused and are crossed with the first beam of pulse lasers, gas plumes generated after the first pulse lasers are gasified and resolved are ionized, and ionized ions fly through a flight tube, the mass spectrum of acriflavine in the mouse kidney tissue is shown in figure 1.
Under the same conditions, the matrix was replaced by a graphite rod, and the mass spectrum obtained was as shown in FIG. 2.
The spectrogram results in fig. 1-2 show that pure acriflavine can be well detected, the signal has a strong peak value, and the effect of adopting the corundum rod as the experimental matrix is better than that of other matrixes such as a graphite rod.
In the aspect of selecting a proper substrate material, the applicant of the invention tries a plurality of common substrate materials including a series of graphite and the like to perform experiments, finds that the effect of the experiments is not ideal, and finally finds that the auxiliary effect of the experiments is the best by using the corundum rod as the experimental substrate through continuous trial and improvement, and the accuracy and the efficiency of the experiments are greatly improved.
Similarly, the present inventors have previously studied several common anticancer and antitumor drugs, acriflavine, nonaphenylacridine, proflavine, acridine, etc., and found that the signal of acriflavine is optimal under mass spectrometry analysis by ionization after laser desorption, and thus the present inventors used acriflavine as a sample for experiments herein.
Example 2
The mass spectrometry imaging method for the biological tissue provided by the embodiment comprises the following steps:
(1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices;
(2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally drying;
(3) and (3) fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer, and entering the mass spectrometer with laser through a sample injection system for detection to obtain a mass spectrogram of acriflavine in the mouse kidney tissue.
And (2) selecting Balb/c male mice in the step (1), and aging for 7-8 weeks.
Fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer in the step (3), and entering the mass spectrometer with laser through a sample injection system for detection, wherein the specific detection process comprises the following steps: starting a mass spectrometer and a three-dimensional platform sample injector, respectively and sequentially emitting pulse lasers by two solid lasers in an ionization mass spectrometer after laser resolution, wherein a first beam of emitted pulse lasers is resolution lasers (infrared resolution lasers), the resolution lasers enter an ionization chamber after being focused and irradiate a mouse kidney tissue slice on a corundum rod, a second beam of pulse lasers is emitted after being delayed for 18-23 mu s, the second beam of pulse lasers is converted into vacuum ultraviolet lasers after being subjected to frequency tripling through a gas pool, the second beam of pulse lasers and the vacuum ultraviolet lasers are focused and separated by a flat convex mirror at a light outlet of the gas pool, the vacuum ultraviolet lasers enter the ionization chamber in a direction parallel to the corundum rod after being focused and are crossed with the first beam of pulse lasers, gas plumes generated after the first pulse lasers are gasified and resolved are ionized, and ionized ions fly through a flight tube, and (3) detecting by a microchannel plate, converting an ion signal into an electric signal by an oscilloscope, and finally processing by matlab software to obtain a required mass spectrum imaging graph, wherein the obtained mass spectrum in the mouse kidney tissue is shown in figure 3.
The mass spectrum of FIG. 3 shows that the signals of other substances (including amino acids, proteins, etc.) in the tissue are mainly concentrated between 0 and 100Da, and by comparing with the spectrum of FIG. 1, we find that the signals of the tissue and the signals of acriflavine do not overlap, which shows that the study on the tissue of the signals of acriflavine does not have any influence on the experimental results.
Example 3
The mass spectrometry imaging method for the biological tissue provided by the embodiment comprises the following steps:
(1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices;
(2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally drying;
(3) 10 mu L of 10-concentration solution is dripped on the kidney tissue section of the mouse-3Standing the g/mL acriflavine solution to uniformly diffuse the acriflavine solution;
(4) and (3) fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer, and entering the mass spectrometer with laser through a sample injection system for detection to obtain a mass spectrogram of acriflavine in the mouse kidney tissue.
And (2) selecting Balb/c male mice in the step (1), and aging for 7-8 weeks.
And (4) standing for 15-25 min in the step (3) to enable the dispersion to be uniform.
Fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer in the step (4), and entering the mass spectrometer with laser through a sample injection system for detection, wherein the specific detection process comprises the following steps: starting a mass spectrometer and a three-dimensional platform sample injector, respectively and sequentially emitting pulse lasers by two solid lasers in an ionization mass spectrometer after laser resolution, wherein a first beam of emitted pulse lasers is resolution lasers, the resolution lasers enter an ionization chamber after being focused and irradiate a mouse kidney tissue slice on a corundum rod, and then a second beam of pulse lasers is emitted after being delayed for 18-23 mu s, the second beam of pulse lasers is converted into vacuum ultraviolet lasers after being subjected to frequency tripling by a gas pool, the second beam of pulse lasers and the vacuum ultraviolet lasers are separated by focusing of a plano-convex mirror at a light outlet of the gas pool, the vacuum ultraviolet lasers enter the ionization chamber in a direction parallel to the corundum rod after being focused and are crossed with the first beam of pulse lasers, gas plumes generated after the first beam of pulse lasers are gasified and resolved are ionized, and ionized ions fly through a flight tube and are detected by a microchannel plate, and converting the ion signals into electric signals through an oscilloscope, and finally processing the electric signals through matlab software to obtain a required mass spectrum imaging graph as shown in FIG. 4.
In FIG. 4, the left image is a section of mouse kidney tissue added with acriflavine solution dropwise, and the right image is a corresponding image of mass spectrum.
By comparing the left image with the right image, we can find that the mass spectrum imaging image of the right image well shows the distribution of acriflavine on the mouse kidney tissue section, which indicates that the novel biological tissue imaging method of the present invention is completely feasible.
The invention is not limited to the specific embodiments described above, which are intended to illustrate the use of the invention in detail, and functionally equivalent production methods and technical details are part of the disclosure. In fact, a person skilled in the art, on the basis of the preceding description, will be able to find different modifications according to his own needs, which modifications are intended to be within the scope of the claims appended hereto.
Claims (5)
1. A biological tissue mass spectrum imaging method is characterized by comprising the following steps:
(1) selecting in vitro mouse kidney tissues, freezing and slicing to obtain mouse kidney tissue slices;
(2) selecting a corundum rod, adhering the mouse kidney tissue slice obtained in the step (1) to the corundum rod, and naturally air-drying;
(3) dropwise adding acriflavine solution to the mouse kidney tissue slice, and standing to uniformly diffuse;
(4) placing the corundum rod adhered with the mouse kidney tissue section dropwise added with the acriflavine solution in a mass spectrometer with laser for detection, and processing mass spectrum data ionized after laser analysis by software to obtain a mass spectrum imaging graph of the mouse kidney tissue containing acriflavine;
fixing the corundum rod adhered with the mouse kidney tissue slice on a sample injection rod of a mass spectrometer in the step (4), and entering the mass spectrometer with laser through a sample injection system for detection, wherein the specific detection process comprises the following steps: starting a mass spectrometer and a three-dimensional platform sample injector, respectively and sequentially emitting pulse lasers by two solid lasers in an ionization mass spectrometer after laser analysis, wherein a first beam of emitted pulse lasers is analyzed lasers, the analyzed lasers enter an ionization chamber after being focused and irradiate on a mouse kidney tissue slice on a corundum rod, and then a second beam of pulse lasers is emitted after being delayed by 18-23 mu s, the second beam of pulse lasers is converted into vacuum ultraviolet lasers after being subjected to frequency tripling in a gas pool, the second beam of pulse lasers and the vacuum ultraviolet lasers are separated by focusing of a plano-convex mirror at a light outlet of the gas pool, the vacuum ultraviolet lasers enter the ionization chamber in a direction parallel to the corundum rod after being focused and are crossed with the first beam of pulse lasers, gas plumes generated after the first beam of pulse lasers are gasified and analyzed are ionized, and the ionized ions fly through a flight tube and are detected by a microchannel plate, and converting the ion signals into electric signals through an oscilloscope, and finally processing the electric signals through matlab software to obtain a required mass spectrum imaging graph.
2. The method of mass spectrometry imaging of biological tissue of claim 1, wherein: and (2) selecting Balb/c male mice in the step (1), and aging for 7-8 weeks.
3. The method of mass spectrometry imaging of biological tissue of claim 1, wherein: the concentration of the acriflavine solution in the step (3) is 10-3g/mL。
4. The method of mass spectrometry imaging of biological tissue of claim 1, wherein: and (4) standing for 15-25 min in the step (3) to enable the dispersion to be uniform.
5. The method of mass spectrometry imaging of biological tissue of claim 1, wherein: and (4) processing the ionized mass spectrum data after laser analysis in the step (4) by matlab software to obtain a mass spectrum imaging graph of the mouse kidney tissue containing acriflavine.
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| CN109813796B (en) * | 2019-03-05 | 2020-11-17 | 中国农业科学院农业质量标准与检测技术研究所 | Mass spectrum imaging method for forchlorfenuron in muskmelon tissues |
| CN110243921B (en) * | 2019-06-28 | 2023-04-18 | 杭州汇健科技有限公司 | Rapid tumor tissue discrimination method based on tissue surface lipid fingerprint spectrogram |
| JP6876881B1 (en) * | 2019-06-28 | 2021-05-26 | 旭化成株式会社 | Imaging samples for mass spectrometers and their manufacturing methods |
| CN111239238B (en) * | 2020-02-03 | 2021-01-26 | 华南农业大学 | Rapid mass spectrometry imaging method for tissue sample |
| CN111796041B (en) * | 2020-08-06 | 2022-09-30 | 中国检验检疫科学研究院 | Imaging detection kit and method for veterinary drug distribution in aquatic products |
| CN112162028A (en) * | 2020-09-29 | 2021-01-01 | 中国农业科学院农业质量标准与检测技术研究所 | Mass spectrum imaging method for vitamin C in strawberry tissue |
| CN114460163B (en) * | 2022-02-11 | 2023-03-03 | 河北师范大学 | MALDI mass spectrometry imaging method for detecting iron content of biological tissue |
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