CN204903549U - Hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box - Google Patents
Hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box Download PDFInfo
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- CN204903549U CN204903549U CN201520610195.1U CN201520610195U CN204903549U CN 204903549 U CN204903549 U CN 204903549U CN 201520610195 U CN201520610195 U CN 201520610195U CN 204903549 U CN204903549 U CN 204903549U
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Abstract
The utility model provides a hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box, the box body includes upper cover and lower cover, on cover to be equipped with and drip an appearance mouthful and a window, be equipped with the test paper strip in the box body, the test paper strip includes the bottom plate, the bottom plate is including dripping appearance district and detection zone, be equipped with first mark pad between a detection zone and the appearance district, first mark pad overlap joint second mark pad, second mark pad overlap joint sample pad, sample pad overlap joint non -dry adhesive layer, the detection zone upper surface covers nitrocellulose membranes, the last peridium of nitrocellulose membranes has for line antibody of hepatitis c viral antigen and matter accuse, be equipped with the pad that absorbs water on the bottom plate of detection zone rear end, nitrocellulose membranes is the first mark pad of overlap joint and the pad that absorbs water respectively. The utility model discloses an increase mark pad and the design of non -setting adhesive and special barbola work, effectively improved detectivity.
Description
Technical field
The utility model relates to C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box.
Background technology
C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box common at present adopts individual layer label pad and sample pad overlap joint, sample can affect mark effect through the flow velocity of individual layer label pad is too fast, and existing kit also fails there is good performance in mark degree of accuracy and sample flow control.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box, can promote mark effect, Control Assay flow velocity, improve and detect degree of accuracy.
The technical scheme that the utility model technical solution problem adopts is: C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box, comprise box body, described box body comprises upper cover and lower cover, cover on described to be provided with and drip sample mouth and form, test strips is provided with in described box body, described test strips comprises base plate, described base plate comprises Di Yang district and detection zone, described Di Yang district is positioned at described below of dripping sample mouth, described detection zone is positioned at the below of described form, the first label pad is provided with between described detection zone and described Di Yang district, above the front end of described first label pad, overlap joint is provided with the second label pad, above the front end of described second label pad, overlap joint is provided with sample pad, above the rear end of described sample pad, overlap joint is provided with non-drying glue layer, described detection zone upper surface is coated with nitrocellulose filter, described nitrocellulose filter is coated with respectively C hepatitis virus antigen and nature controlling line antibody, the base plate of rear end, described detection zone is provided with adsorptive pads, the two ends of described nitrocellulose filter overlap the first label pad and described adsorptive pads respectively.
While employing technique scheme, the utility model can also adopt or combine and adopt following further technical scheme:
The outer ledge of described sample pad and described adsorptive pads aligns with the front-end and back-end of described base plate respectively.
The lap of splice of described first label pad and described second label pad is 5-6mm.
The lap of splice of described first label pad and described nitrocellulose filter is 1mm.
One end overlap joint sample pad of described non-drying glue layer, other end overlap joint is to described nitrocellulose filter, and described non-drying glue layer covers in described first label pad and described second label pad.
The beneficial effects of the utility model are: this kit is applicable to the qualitative detection of the antibody of HCV in human serum, blood plasma or whole blood sample, and whether auxiliary diagnosis has infected hepatitis C virus, are applicable to the detection of clinical and on-the-spot primary dcreening operation.
The utility model, by increasing the design of label pad and adhesive sticker and special barbola work, effectively improves detection sensitivity.This kit and JS3000 immunity analysis instrument support the use, and change traditional gold-marking immunity chromatography can only do qualitative analysis situation by visual observations testing result, embody very large advantage in accuracy, convenience and automaticity simultaneously.
Accompanying drawing explanation
Fig. 1 is outside drawing of the present utility model.
Fig. 2 is the structural drawing of test strips of the present utility model.
Embodiment
With reference to accompanying drawing.
Kit of the present utility model comprises box body, box body comprises upper cover 10 and lower cover 11, upper cover 10 is provided with and drips sample mouth 12 and form 13, test strips is provided with in box body, test strips comprises base plate 9, base plate 9 comprises Di Yang district 14 and detection zone 15, Di Yang district 14 is positioned at the below of dripping sample mouth 12, detection zone 15 is positioned at the below of form 13, the first label pad 4 is provided with between detection zone 15 and Di Yang district 14, above the front end of the first label pad 4, overlap joint is provided with the second label pad 3, above the front end of the second label pad 3, overlap joint is provided with sample pad 1, above the rear end of sample pad 1, overlap joint is provided with non-drying glue layer 2, the upper surface of detection zone 15 is coated with nitrocellulose filter 5, nitrocellulose filter 5 is coated with respectively C hepatitis virus antigen and nature controlling line antibody, bag is provided with detection line 6 and nature controlling line 7 respectively accordingly by part, the base plate 9 of rear end, detection zone 15 is provided with adsorptive pads 8, the two ends of nitrocellulose filter 5 overlap the first label pad 4 and adsorptive pads 8 respectively.
The outer ledge of sample pad 1 and adsorptive pads 8 respectively with the justify align of base plate 9.
The lap of splice of the first label pad 4 and the second label pad 3 is 5-6mm.
The lap of splice of the first label pad 4 and nitrocellulose filter 5 is 1mm.
One end overlap joint sample pad 1 of non-drying glue layer 2, other end overlap joint is to nitrocellulose filter 5, non-drying glue layer 2 is 1mm with the lap of splice of nitrocellulose filter 5, the middle part of non-drying glue layer 2 covers in the first mark basic unit 4 and the second mark basic unit 3, nitrocellulose filter 5 is pasted onto on the detection zone 15 of base plate 9, can see detection line 6 in detection zone and nature controlling line 7 through form 13.
The preparation method of kit of the present utility model is as follows:
C hepatitis virus antigen and sheep anti-mouse igg use 0.01M phosphate buffer (pH7.2-7.4) to be diluted to 0.4mg/ml-1.0mg/ml respectively, and successively this mixed liquor is drawn on the nitrocellulose filter that sticks on PV plate with the discharge rate of 1.0-1.5ul/cm with drawing film machine, the colloid gold label potpourri of gold mark HCV antigen and gold mark mouse IgG is sprayed at label pad.After 37 DEG C or 45 DEG C are dried, stick on request on base plate, thieving paper and sample pad are also bonded at relevant position, then after being cut into 6cm*4mm size with cutting cutter, loading is got stuck, compacting.
The using method of kit of the present utility model is as follows:
The detection of HCV antibody
1, testing tool: automatic lmunoassays analyzer (JS3000)
2, testing process and method:
(1) necessary complete reading operation instructions before testing, balance the check-out console of original packing, detection damping fluid and sample to be tested to room temperature before using; And check-out console, detection damping fluid and sample to be tested are respectively charged into JS3000 relevant position, and carry out bar code scan.
(2) sample this 96ul, get and detect damping fluid 36ul, both mixings, then get 110ul and add in check-out console well, and after 15 minutes, instrument automatic transport to optical module station carries out gold mark signal measuring, thus result is presented on instrument display screen.
(3) computing method: multiple spot is calibrated, and using sample function as computation schema, the value according to signal and calibration object makes reagent/response curve, and sample content can calculate on dosage/response curve according to its signal value, thus determines yin and yang attribute.
Claims (5)
1. C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box, comprise box body, described box body comprises upper cover and lower cover, cover on described to be provided with and drip sample mouth and form, it is characterized in that: in described box body, be provided with test strips, described test strips comprises base plate, described base plate comprises Di Yang district and detection zone, described Di Yang district is positioned at described below of dripping sample mouth, described detection zone is positioned at the below of described form, the first label pad is provided with between described detection zone and described Di Yang district, above the front end of described first label pad, overlap joint is provided with the second label pad, above the front end of described second label pad, overlap joint is provided with sample pad, above the rear end of described sample pad, overlap joint is provided with non-drying glue layer, described detection zone upper surface is coated with nitrocellulose filter, described nitrocellulose filter is coated with respectively C hepatitis virus antigen and nature controlling line antibody, the base plate of rear end, described detection zone is provided with adsorptive pads, the two ends of described nitrocellulose filter overlap the first label pad and described adsorptive pads respectively.
2. C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box as claimed in claim 1, is characterized in that: the outer ledge of described sample pad and described adsorptive pads aligns with the front-end and back-end of described base plate respectively.
3. C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box as claimed in claim 1, is characterized in that: the lap of splice of described first label pad and described second label pad is 5-6mm.
4. C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box as claimed in claim 1, is characterized in that: the lap of splice of described first label pad and described nitrocellulose filter is 1mm.
5. C hepatitis virus antigen colloidal gold immunochromatographiassay assay reagent box as claimed in claim 1, it is characterized in that: one end overlap joint sample pad of described non-drying glue layer, other end overlap joint is to described nitrocellulose filter, and described non-drying glue layer covers in described first label pad and described second label pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520610195.1U CN204903549U (en) | 2015-08-13 | 2015-08-13 | Hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520610195.1U CN204903549U (en) | 2015-08-13 | 2015-08-13 | Hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box |
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| Publication Number | Publication Date |
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| CN204903549U true CN204903549U (en) | 2015-12-23 |
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| CN201520610195.1U Active CN204903549U (en) | 2015-08-13 | 2015-08-13 | Hepatitis c viral antigen colloidal gold immunoassay chromatography detect reagent box |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732347A (en) * | 2018-05-23 | 2018-11-02 | 江苏维尔生物科技有限公司 | A kind of detection kit and preparation method thereof detecting HCV antibody in saliva |
-
2015
- 2015-08-13 CN CN201520610195.1U patent/CN204903549U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732347A (en) * | 2018-05-23 | 2018-11-02 | 江苏维尔生物科技有限公司 | A kind of detection kit and preparation method thereof detecting HCV antibody in saliva |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 10, building 311100, building 519, Xingguo Road, Yuhang Economic Development Zone, Zhejiang, Hangzhou Patentee after: The Sheng Tai biotechnology Limited by Share Ltd Address before: 311100 No. 10, building 519, Xingguo Road, Qianjiang Economic Development Zone, Zhejiang, Hangzhou Patentee before: HANGZHOU JOINSTAR BIOTECHNOLOGY CO., LTD. |