CN204579567U - A kind of fast-collection device of sporangiocyst class nematode sporangiocyst - Google Patents
A kind of fast-collection device of sporangiocyst class nematode sporangiocyst Download PDFInfo
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- CN204579567U CN204579567U CN201520175300.3U CN201520175300U CN204579567U CN 204579567 U CN204579567 U CN 204579567U CN 201520175300 U CN201520175300 U CN 201520175300U CN 204579567 U CN204579567 U CN 204579567U
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- sporangiocyst
- collection
- fast
- screen cloth
- collection device
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- 241000244206 Nematoda Species 0.000 title claims abstract description 15
- 239000002699 waste material Substances 0.000 claims abstract description 33
- 239000004744 fabric Substances 0.000 claims abstract description 27
- 238000000967 suction filtration Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 description 12
- 206010011732 Cyst Diseases 0.000 description 11
- 208000031513 cyst Diseases 0.000 description 11
- 241000244203 Caenorhabditis elegans Species 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 6
- 210000003491 skin Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 239000002689 soil Substances 0.000 description 4
- 239000012780 transparent material Substances 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000482313 Globodera ellingtonae Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- 244000176051 Apios tuberosa Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001480224 Heterodera Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 206010062701 Nematodiasis Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 235000010716 Vigna mungo Nutrition 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 229920001617 Vinyon Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of fast-collection device of sporangiocyst class nematode sporangiocyst, comprise filter and Suction filtration device, described Suction filtration device comprises collection funnel and the bottom waste stream receiving flask at top, described collection funnel comprises bucket body and leakage pipe, described bucket body top is provided with the screen cloth of tiling, described bottom, leakage pipe portion and receiving flask are tightly connected, and the sidepiece of described collection funnel or the sidepiece of waste collection bottle are provided with the blast pipe connecting air extractor; Described filter has two-layer screen cloth, and the order number of upper strata screen cloth is 10-20 order, and the order number of lower screen is 60-80 order.Sporangiocyst gathering unit of the present utility model, has time saving and energy saving, sporangiocyst and is easy to collect the advantages such as counting.
Description
Technical field
A kind of gathering unit of the utility model system, mainly for the fast-collection device of sporangiocyst class nematode sporangiocyst.
Background technology
The crop cyst nematode disease caused by cyst roundworm (Heterodera spp.) is a most important class nematodiasis on the crop of Temperate Region in China.Cyst roundworm is the settling down property plant endoparasitism nematode that a class soil passes, complete and generally need 30-35 days a history of life, fertility is strong, the main worm state that such nematode of sporangiocyst system of formation survives the winter, there are extremely strong vitality and adaptability widely, generally can survive in soil more than 9 years.
On the other hand, cyst roundworm causes the production loss of many important crops, and these crops comprise cereal crop, paddy rice, potato and soybean.The hazard symptoms main manifestations of crop field upper part is the short jaundice symptom of poor growth and yellowing leaf, and the hazard symptoms main manifestations of under ground portion is that root system is undeveloped, because root system is impaired, fully can not absorb rich water, and time serious, root system rots.Visible milky or yellow particle (i.e. sporangiocyst) on fibrous root.
The whole history of life of cyst roundworm is experienced 4 times and casts off a skin.First, first-instar young (First-stagejuveniles, J1s) casts off a skin into J2s in the ovum (Egg) of sporangiocyst (Cyst).J2s infects host's root and induced synthesis takes food site---plasomidum (Syncytium).J2s obtains nutriment from plasomidum, and polypide is expanded and casts off a skin and develops into J3s.J3s is again casted off a skin at development later stage and is developed into J4s (J4 male worm or female worm).J4s polypide continues to grow expands and casts off a skin into male worm adult or female worm adult.Female worm adult breaks through host's root tissue epidermis, but head is still fixed on and takes food that site Cell relay is continuous to be taken food; Male worm adult then dissociates root complete mating with female worm.Ovum starts to be formed in female polypide.When after female worm death, its polypide is transformed into the firm and sporangiocyst of tool protectiveness of quality, protection ovum wherein.Sporangiocyst regularly and in quantitative detection soil and on root system of plant is the basis of control cyst nematode disease.
The separating method of existing cyst roundworm sporangiocyst is a lot, and conventional comprises sucrose centrifugal separation, Fenwick levitron partition method and direct sieve method excessively.But the sporangiocyst that these separating methods finally obtain is all in a liquid, is often mixed with impurity in liquid, impact counting and collection.Conventional method pours in the funnel containing filter paper by collection liquid, after water is filtered dry, collects the sporangiocyst on filter paper.This method wastes time and energy, counting difficulty.
Utility model content
The utility model provides the sporangiocyst fast-collection device of a kind of sporangiocyst class nematode, not only easy and simple to handle and also solve sporangiocyst collect time, waste time and energy, counting difficulty problem.
For achieving the above object, the utility model provides following technical scheme:
A kind of fast-collection device of sporangiocyst class nematode sporangiocyst, comprise filter and Suction filtration device, described Suction filtration device comprises collection funnel and the bottom waste stream receiving flask at top, described collection funnel comprises bucket body and leakage pipe, described bucket body top is provided with the screen cloth of tiling, described bottom, leakage pipe portion and receiving flask are tightly connected, and the sidepiece of described collection funnel or the sidepiece of waste collection bottle are provided with the blast pipe connecting air extractor; Described filter has two-layer screen cloth, and the order number of upper strata screen cloth is 10-20 order, and the order number of lower screen is 60-80 order.
The conveniently counting of sporangiocyst, described screen cloth tiles filter paper, and filter paper has graticule line, and filter paper is divided into some regions by the graticule line on filter paper, sporangiocyst is counted under the microscope convenient.
As preferably, described screen cloth is provided with the detent mechanism for fixing filter paper.
As preferably, described detent mechanism is the convex pin that can penetrate filter paper, and namely convex pin facilitates the fixing of filter paper, facilitates again filter paper to take off from screen cloth.
In use, in order to make gathering unit hold reliably, be simultaneously convenient to disassembly, cleaning between Suction filtration device and filter, as preferably, described leakage pipe is threaded with waste collection bottle; In order to prevent in suction filtration process gathering unit gas leakage, as preferably, the junction of leakage pipe and waste collection bottle is provided with seal washer.
As preferably, described leakage pipe bottom is provided with external screw thread, and described waste collection mouth cylindrical part is provided with the internal thread matched.The inside of waste collection bottle is inserted in the bottom of leakage pipe, and both are threaded connection.
In order to prevent the waste liquid in waste collection bottle to be drawn in air extractor, described blast pipe is inclined upwardly.
For the ease of observing the liquid level of waste liquid in waste collection bottle and toppling over waste liquid in time, as preferably, described waste collection bottle is that transparent material is made.Collection funnel also can adopt transparent material to make.
Compared with prior art, the utility model has following beneficial effect:
The utility model carries out collecting by filtration by the two-layer screen cloth of filter to the sporangiocyst of cyst roundworm, the impurity that upper strata screen cloth filtering plant tissue etc. are larger, and less sporangiocyst filters lower screen; The sporangiocyst collected, again through Suction filtration device suction filtration, removes the moisture carried.Conveniently take out the sporangiocyst of collection, the screen cloth of Suction filtration device can lay filter paper, after the moisture on filter paper blots by air extractor, under filter paper can being transferred directly to microscope, observation counting is carried out to the sporangiocyst on filter paper.Sporangiocyst gathering unit of the present utility model, has time saving and energy saving, sporangiocyst and is easy to collect the advantages such as counting.
Accompanying drawing explanation
Fig. 1 is filter apparatus configuration schematic diagram of the present utility model;
Fig. 2 is Suction filtration device structural representation of the present utility model;
Fig. 3 is the cutaway view in A portion in Fig. 2.
Embodiment
In order to further set forth the technological means and effect thereof that the utility model takes, below in conjunction with embodiment and accompanying drawing thereof, the fast-collection device to the utility model cyst roundworm sporangiocyst is described in further detail.
The fast-collection device of the utility model sporangiocyst class nematode sporangiocyst, comprises filter 1 and Suction filtration device 2.A kind of filter of embodiment as shown in Figure 1, filter has two-layer screen cloth: upper strata screen cloth 11 and lower screen 12, and the sieve aperture of upper strata screen cloth 11 is 20 orders, and the sieve aperture of lower screen 12 is 60 orders, for removably connecting between two sieves, can separate after having filtered.
Fig. 2 is the Suction filtration device 2 of the fast-collection device of the utility model cyst roundworm sporangiocyst, comprises collection funnel 22 and the waste collection bottle 23 of socket mutually.Collection funnel 2 is in funnel-form, and upper end is conical bucket body, and the lower ending opening place of bucket body is connected to leakage pipe, and bucket body upper end is provided with the screen cloth 25 that level is laid.Screen cloth 25 can be laid the filter paper with grid lines, screen cloth 25 is provided with the convex pin (not shown) of fixing filter paper simultaneously, and convex pin can penetrate filter paper and be fixed on screen cloth.
The tapered ampuliform of waste collection bottle 23, the diameter of upper end bottleneck is slightly larger than the diameter of leakage pipe.As shown in Figure 3, connected by thread seal between collection funnel 22 and waste collection bottle 23, the bottle mouth position inwall of waste collection bottle 23 there is internal thread, the lower end of leakage pipe is inserted in waste collection bottle 23, the leakage pipe position corresponding with bottleneck is provided with the external screw thread matched with bottleneck, both are connected by the thread seal cooperatively interacted, strengthen the fast-collection device steadiness in use of this cyst roundworm sporangiocyst, also facilitate the dismounting between collection funnel 22 and waste collection bottle 23 simultaneously, be convenient to the cleaning of device inside and toppling over of waste liquid.
Embodiment of the present utility model is provided with the blast pipe 24 be connected with air extractor on the sidewall of collection funnel 23, can be used for the sporangiocyst that collected by suction arrives, removing moisture, is convenient to observe counting; In order to prevent, in timer gas leakage of bleeding, at collection funnel 22 and the junction of waste collection bottle 23, seal washer 3 being housed.
Conveniently observe the liquid level of waste liquid in waste collection bottle 23, topple over waste liquid in time, the material of the waste collection bottle 23 of the utility model embodiment is transparent vinyon (PE), also can adopt other transparent materials, as glass etc.Other parts of certain embodiment also can adopt transparent material to make, and are convenient to the inner case observing gathering unit.
Using method of the present utility model is:
To the sporangiocyst in soil first through levitron partition method with directly cross after sieve method etc. obtains sporangiocyst and plant root tissue suspension that some are tiny, collect by following method.
Filter of the present utility model is first used to collect sporangiocyst: to be had by parasitism the plant root tissue of cyst roundworm to be placed on upper strata screen cloth 11 (sieve aperture 20 order), female worm on root and sporangiocyst are flushed on lower screen 12 (sieve aperture 60 order) with strong current, the impurity that plant root tissue etc. are larger is filtered on upper strata screen cloth 11; Discard the root tissue etc. on upper strata screen cloth 11, and the water flushing such as the female worm on lower screen 12 and sporangiocyst is transferred in beaker.
Re-use Suction filtration device of the present utility model and suction filtration is carried out to the sporangiocyst collected: the sporangiocyst liquid in beaker is poured in collection funnel 22 of the present utility model, blast pipe 24 is connected on air extractor and bleeds, after the filter paper on screen cloth is drained, take off filter paper, be placed in basis of microscopic observation sporangiocyst and count.
The foregoing is only better implementation example of the present utility model, be not limited to the utility model, all within the utility model spirit and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (6)
1. the fast-collection device of a sporangiocyst class nematode sporangiocyst, it is characterized in that, comprise filter and Suction filtration device, described Suction filtration device comprises collection funnel and the bottom waste stream receiving flask at top, described collection funnel comprises bucket body and leakage pipe, described bucket body top is provided with the screen cloth of tiling, and described bottom, leakage pipe portion and waste collection bottle are tightly connected, and the sidepiece of described collection funnel or the sidepiece of waste collection bottle are provided with the blast pipe connecting air extractor; Described filter has two-layer screen cloth, and the order number of upper strata screen cloth is 10-20 order, and the order number of lower screen is 60-80 order.
2. the fast-collection device of sporangiocyst class nematode sporangiocyst as claimed in claim 1, it is characterized in that, described screen cloth is provided with the detent mechanism for fixing filter paper.
3. the fast-collection device of sporangiocyst class nematode sporangiocyst as claimed in claim 2, it is characterized in that, described detent mechanism is the convex pin that can penetrate filter paper.
4. the fast-collection device of sporangiocyst class nematode sporangiocyst as claimed in claim 1, it is characterized in that, described leakage pipe is threaded with waste collection bottle, and junction is provided with seal washer.
5. the fast-collection device of sporangiocyst class nematode sporangiocyst as claimed in claim 4, it is characterized in that, described leakage pipe bottom is provided with external screw thread, and described waste collection mouth cylindrical part is provided with the internal thread matched.
6. the fast-collection device of sporangiocyst class nematode sporangiocyst as claimed in claim 1, it is characterized in that, described blast pipe is inclined upwardly.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520175300.3U CN204579567U (en) | 2015-03-26 | 2015-03-26 | A kind of fast-collection device of sporangiocyst class nematode sporangiocyst |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520175300.3U CN204579567U (en) | 2015-03-26 | 2015-03-26 | A kind of fast-collection device of sporangiocyst class nematode sporangiocyst |
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| CN204579567U true CN204579567U (en) | 2015-08-26 |
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| CN201520175300.3U Expired - Fee Related CN204579567U (en) | 2015-03-26 | 2015-03-26 | A kind of fast-collection device of sporangiocyst class nematode sporangiocyst |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746098A (en) * | 2016-03-30 | 2016-07-13 | 四川省农业科学院土壤肥料研究所 | Ophiocordyceps sinensis spore collection device and collection method |
| CN108844800A (en) * | 2018-09-10 | 2018-11-20 | 江西业力医疗器械有限公司 | A kind of disposable excrement parasitic ovum acquisition pelletizer |
-
2015
- 2015-03-26 CN CN201520175300.3U patent/CN204579567U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746098A (en) * | 2016-03-30 | 2016-07-13 | 四川省农业科学院土壤肥料研究所 | Ophiocordyceps sinensis spore collection device and collection method |
| CN105746098B (en) * | 2016-03-30 | 2017-11-14 | 四川省农业科学院土壤肥料研究所 | Cordyceps sinensis spore collection method |
| CN108844800A (en) * | 2018-09-10 | 2018-11-20 | 江西业力医疗器械有限公司 | A kind of disposable excrement parasitic ovum acquisition pelletizer |
| CN108844800B (en) * | 2018-09-10 | 2023-11-03 | 广州市准好生物科技有限公司 | Disposable parasitic insect egg of excrement gathers film-making device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150826 Termination date: 20210326 |
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| CF01 | Termination of patent right due to non-payment of annual fee |