CN204302069U - A kind of device that simultaneously can extract sugar chain - Google Patents
A kind of device that simultaneously can extract sugar chain Download PDFInfo
- Publication number
- CN204302069U CN204302069U CN201420581008.7U CN201420581008U CN204302069U CN 204302069 U CN204302069 U CN 204302069U CN 201420581008 U CN201420581008 U CN 201420581008U CN 204302069 U CN204302069 U CN 204302069U
- Authority
- CN
- China
- Prior art keywords
- main body
- angle main
- turning left
- angle
- turning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of device that simultaneously can extract sugar chain, comprise support, angle main body of turning left, angle main body of turning right, ship type main body, pellicle, gasket seal, long soft-pipe and short flexible pipe, described angle main body of turning left is located on support with angle main frame of turning right, described angle main body of turning left is provided with corresponding hole with angle main body of turning right, and two corresponding holes are by long soft-pipe and short soft pipe connection; Be provided with ship type main body at described angle main body upper bottom surface of turning left, described ship type main body is provided with the hole corresponding with angle main body of turning left, and is provided with pellicle turning left between angle main body and ship type main body, is respectively equipped with gasket seal at the upper bottom surface of pellicle.The utility model is rational in infrastructure, practical and convenient, simplify operation, can the multiple identical or different kind sample of same time-triggered protocol, the column volume in each hole can be consistent, make component contained by sample to be separated within the basically identical time and to collect like this, especially can eliminate accidental error for parallel sample.
Description
Technical field
The utility model relates to a kind of device of Experiment of Analytical Chemistry, specifically a kind of device that simultaneously can extract sugar chain, is especially a kind ofly applicable to the device that sugar chain can be extracted in 96 holes simultaneously.
Background technology
One of key of sugar group research is the acquisition to sugar chain, namely carries out separation and purification to the sugar chain that glycoprotein discharges through enzyme process or chemical method.How obtaining sugar chain is a sugar group Epidemiological Analysis technical difficult problem, and at present, preparation method many employings enzymatic isolation method of sugar chain or Chemical releases method on glycoprotein, this separation and purification just determining sugar chain mainly comprises other small molecular weight impurity of de-albumen, desalination and removing.
The main separation method using bag filter and activated-charcoal column to combine carries out separation and purification to sample at present.Dialysis utilizes solution concentration diffusion effect, by material little for molecular weight as salt ion, oligonucleotide chain etc. penetrate in distilled water outside bag or dislysate, finite concentration can be kept poor, thus remove small-molecule substance by constantly changing water.Active carbon belongs to non-polar adsorbent, has certain adsorption capacity to sugar chain, is suitable for being separated sugar chain and salt ion.But the method is generally carry out separation and purification operation to simple sample.When carrying out Multi-example or batch sample separation and purification experiment, multiple bag filter and activated-charcoal column need be used to operate or a bag filter and activated-charcoal column operate repeatedly simultaneously, existence expends time in, complex operation, working face are excessive and labour intensity crosses the problems such as high, causes operating efficiency too low.
Summary of the invention
The purpose of this utility model overcomes the deficiencies in the prior art, provides a kind of sugar chain extraction element that simultaneously can realize Multi-example or batch sample separation and purification, and this device can obtain the sugar chain of maximum 96 samples release simultaneously.
The technical solution of the utility model is:
A kind of device that simultaneously can extract sugar chain, comprise support, angle main body of turning left, angle main body of turning right, ship type main body, pellicle, gasket seal, long soft-pipe and short flexible pipe, described angle main body of turning left is located on support with angle main frame of turning right, described angle main body of turning left is provided with corresponding hole with angle main body of turning right, two corresponding holes are by long soft-pipe and short soft pipe connection, in close main body side, angle of turning left, all long soft-pipes are enclosed with METAL HEATING PROCESS block, and all short hose package are wrapped with metal cooling block; Be provided with ship type main body at described angle main body upper bottom surface of turning left, described ship type main body is provided with the hole corresponding with angle main body of turning left, and is provided with pellicle turning left between angle main body and ship type main body, is respectively equipped with gasket seal at the upper bottom surface of pellicle.
Preferably, the upper bottom surface of described angle main body of turning right is higher than the upper bottom surface of angle main body of turning left, to ensure that liquid can be full of flexible pipe and angle main body of turning left smoothly from atmospheric pressure can be utilized during the hole application of sample of angle main body of turning right to ensure, between the liquid level of angle main body of simultaneously guaranteeing to turn left and pellicle, there is not bubble.Described angle main body of turning left, angle main body of turning right are symmetrical is respectively provided with 96 identical holes of size, and described hole is according to the format permutation of 96 porocyte culture plates.
Described gasket seal is provided with the hole corresponding with angle main body of turning left, and described hole, according to the format permutation of 96 porocyte culture plates, can be connected with 96 holes of angle main body of turning left with the ship type main body contacted up and down.The material of described gasket seal is waterproof sponge, and gasket seal can play a cushioning effect, the sealing guaranteed ship type main body and turn left between the main body of angle, ensures that the solution No leakage phenomenon in hole occurs.
In each hole of described turn left angle main body and angle main body of turning right, the mouth of pipe of long soft-pipe is higher than the mouth of pipe of short flexible pipe; The bottom of described long soft-pipe is lower than the bottom of short flexible pipe.All long soft-pipes are being enclosed with METAL HEATING PROCESS block near main body side, angle of turning left, and all short flexible pipes are being enclosed with metal cooling block near main body side, angle of turning left.Thus realize cold and hot convection current in the main body of left and right turning, in the hole that in the hole of the angle main body that ensures to turn right, d/d sugar chain transfers to angle main body of turning left and then pass pellicle by the charcoal absorption in ship type main body.Described long soft-pipe and short flexible pipe are U-shaped flexible pipe.
Four angles of described ship type main body and angle main body of turning left are respectively equipped with installing hole, and ship type main body and angle main body of turning left are fixed on support by column.Preferably, described installing hole is processed with internal thread, and described column is processed with external screw thread, and ship type main body, angle main body of turning left also tightly are fixed on support by the top nut of column, between described nut and ship type main body, be provided with pad.
Described pellicle is individual layer dialysis membrane.The aperture of pellicle can experimentally demand regulate, and its molecular cut off MWCO should be less than the molecular weight of the glycoprotein being extracted sugar chain, ensures that glycoprotein cannot pass pellicle.
The beneficial effects of the utility model:
The utility model is rational in infrastructure, practical and convenient, can the multiple identical or different kind sample of same time-triggered protocol, the column volume in each hole can be consistent, and flow velocity can be kept consistent, make component contained by sample to be separated within the basically identical time and to collect like this, especially can eliminate accidental error for parallel sample.The utility model simplifies operation, is applicable to small-molecule substance separation and purification experiment, is the optimal separation tools for purification of sugar chain analysis design mothod during sugar group is learned.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the scheme of installation of the utility model hull main body and angle main body of turning left.
In figure, 1-support, 2-turns left angle main body, and 3-turns right angle main body, 4-ship type main body, 5-pellicle, 6-gasket seal, 7-long soft-pipe, the short flexible pipe of 8-, 9-METAL HEATING PROCESS block, 10-metal cooling block, 11-column, 12-nut, 13-pad.
Detailed description of the invention
Below in conjunction with drawings and Examples, the utility model is further described.
As depicted in figs. 1 and 2, a device for sugar chain can be extracted simultaneously, comprise support 1, angle main body 2 of turning left, angle main body 3 of turning right, ship type main body 4, pellicle 5, gasket seal 6, long soft-pipe 7, short flexible pipe 8, METAL HEATING PROCESS block 9, metal cooling block 10 and column 11; Described angle main body 2 and the angle main body 3 of turning right of turning left is erected on support 1, and the upper bottom surface of angle main body 3 of turning right is higher than the upper bottom surface of angle main body 2 of turning left; Described angle main body 2 of turning left is provided with 96 identical holes of corresponding size with angle main body 3 of turning right, and hole is according to the format permutation of 96 porocyte culture plates; Angle main body 2 of turning left two holes corresponding with angle main body 3 of turning right are communicated with short flexible pipe 8 by long soft-pipe 7, and in each hole, the mouth of pipe of long soft-pipe 7 is higher than the mouth of pipe of short flexible pipe 8, and the bottom of long soft-pipe 7 is lower than the bottom of short flexible pipe 8; In close angle main body 2 side of turning left, all long soft-pipes 7 are enclosed with METAL HEATING PROCESS block 9, and all short flexible pipes 8 are enclosed with metal cooling block 10; Ship type main body 4 is provided with at described angle main body 2 upper bottom surface of turning left, described ship type main body 4 is provided with the hole corresponding with angle main body 2 of turning left, be provided with pellicle 5 turning left between angle main body 2 and ship type main body 4, be respectively equipped with gasket seal 6 at the upper bottom surface of pellicle 5, gasket seal 6 is provided with the hole corresponding with angle main body 2 of turning left; Described ship type main body 4 and four angles of angle main body 2 of turning left arrange the female installing hole of processing respectively, ship type main body 4 and angle main body 2 of turning left to be fixed on support 1 by being processed with externally threaded column 11, the top of column 11 is also tight with nut 12, between described nut 12 and ship type main body 4, be provided with pad 13.
Described pellicle 5 is individual layer dialysis membrane.The material of described gasket seal 6 is waterproof sponge.
During use, active carbon can be loaded as required in the hole of ship type main body 4, and the glycoprotein of sugar chain to be released and corresponding enzyme and suitable buffer solution are joined turn right in the hole of angle main body 3.Sugar chain after enzymolysis is through dialysis membrane 5 under the drive of cold cycling, and by the charcoal absorption of ship type main body 4 li, profit at utmost can change the sugar chain in the main hole of absorption left and right turning in this way.Take out the active carbon in ship type main body 4 hole after the completion of reaction, wash out by the sugar chain adsorbed with acetonitrile solution, be spin-dried for rear removal organic solvent can obtain purifying after sugar chain.
Claims (8)
1. one kind can be extracted the device of sugar chain simultaneously, it is characterized in that comprising support, angle main body of turning left, angle main body of turning right, ship type main body, pellicle, gasket seal, long soft-pipe and short flexible pipe, described angle main body of turning left is located on support with angle main frame of turning right, described angle main body of turning left is provided with corresponding hole with angle main body of turning right, two corresponding holes are by long soft-pipe and short soft pipe connection, in close main body side, angle of turning left, all long soft-pipes are enclosed with METAL HEATING PROCESS block, and all short hose package are wrapped with metal cooling block; Be provided with ship type main body at described angle main body upper bottom surface of turning left, described ship type main body is provided with the hole corresponding with angle main body of turning left, and is provided with pellicle turning left between angle main body and ship type main body, is respectively equipped with gasket seal at the upper bottom surface of pellicle.
2. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that the upper bottom surface of upper bottom surface higher than angle main body of turning left of described angle main body of turning right.
3. the device that simultaneously can extract sugar chain according to claim 1, it is characterized in that described angle main body of turning left, angle main body of turning right are symmetrical respectively and be provided with 96 identical holes of size, described hole is according to the format permutation of 96 porocyte culture plates.
4. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described gasket seal is provided with the hole corresponding with angle main body of turning left.
5. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that the mouth of pipe of the mouth of pipe higher than short flexible pipe of long soft-pipe in each hole of described turn left angle main body and angle main body of turning right; The bottom of described long soft-pipe is lower than the bottom of short flexible pipe.
6. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described ship type main body and four angles of angle main body of turning left are respectively equipped with installing hole, and ship type main body and angle main body of turning left are fixed on support by column.
7. the device that simultaneously can extract sugar chain according to claim 6, it is characterized in that described installing hole is processed with internal thread, described column is processed with external screw thread, ship type main body, angle main body of turning left also tightly are fixed on support by the top nut of column, between described nut and ship type main body, be provided with pad.
8. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described pellicle is individual layer dialysis membrane; The material of described gasket seal is waterproof sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420581008.7U CN204302069U (en) | 2014-10-09 | 2014-10-09 | A kind of device that simultaneously can extract sugar chain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420581008.7U CN204302069U (en) | 2014-10-09 | 2014-10-09 | A kind of device that simultaneously can extract sugar chain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204302069U true CN204302069U (en) | 2015-04-29 |
Family
ID=53107600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420581008.7U Withdrawn - After Issue CN204302069U (en) | 2014-10-09 | 2014-10-09 | A kind of device that simultaneously can extract sugar chain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204302069U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104236979A (en) * | 2014-10-09 | 2014-12-24 | 南京农业大学 | Device capable of simultaneously extracting carbohydrate chains |
-
2014
- 2014-10-09 CN CN201420581008.7U patent/CN204302069U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104236979A (en) * | 2014-10-09 | 2014-12-24 | 南京农业大学 | Device capable of simultaneously extracting carbohydrate chains |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203108263U (en) | Double column circulating chromatographic system | |
| CN204302069U (en) | A kind of device that simultaneously can extract sugar chain | |
| CN103157295B (en) | Double-column recycling chromatographic system | |
| CN202555136U (en) | Tail gas purification device for mercuric chloride catalyst production | |
| CN203564815U (en) | Centrifugal solid-phase extraction column | |
| CN103045713A (en) | Method for detecting trace amounts of microbes in a liquid sample containing multiple impurities | |
| CN203668114U (en) | UASB (up-flow anaerobic sludge blanket) reactor | |
| CN104236979A (en) | Device capable of simultaneously extracting carbohydrate chains | |
| WO2005037988A3 (en) | Nucleic acid-adsorbing porous membrane for separating and purifying nucleic acid and apparatus for separating and purifying nucleic acid | |
| CN102060674A (en) | Method for purifying high-purity organic solvent diethyl ether | |
| CN106349055B (en) | A kind of new separating and extracting process of gluconic acid mother liquid of sodium | |
| CN102827045A (en) | Method and apparatus for removing impurity in secondary methionine mother liquor | |
| CN102516112B (en) | Preparation method for chromatographic grade N,N-dimethyl formamide | |
| CN204910866U (en) | Be used for purified chromatographic system of high -purity ganglioside | |
| CN107973711A (en) | A kind of method of Simulation moving bed separating glucose acid mother liquid of sodium | |
| CN102731251B (en) | Purification method of highly pure organic solvent n-butanol | |
| CN105505890A (en) | Water enterovirus separating and purifying method | |
| CN202049080U (en) | Purification pretreatment device for static method specific surface area and pore size analyzer | |
| CN202700137U (en) | Rotary composite jointed solid-phase substrate extraction device | |
| CN205204864U (en) | Sewage automatic check out system | |
| CN212262431U (en) | Waste gas treatment system based on new energy automobile lithium cell recovery processing in-process | |
| CN102040497A (en) | Purification method of high-purity organic solvent butanone | |
| CN204508989U (en) | A kind of experiment baffling layer-stepping permeable reactive wall | |
| CN208577729U (en) | A kind of trace plant specimen samples DNA absorptive collection device | |
| CN202609925U (en) | Low concentration hydrogen recovery device adopting membrane separation and pressure swing adsorption |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20150429 Effective date of abandoning: 20160817 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |