CN104236979A - Device capable of simultaneously extracting carbohydrate chains - Google Patents
Device capable of simultaneously extracting carbohydrate chains Download PDFInfo
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- CN104236979A CN104236979A CN201410529820.XA CN201410529820A CN104236979A CN 104236979 A CN104236979 A CN 104236979A CN 201410529820 A CN201410529820 A CN 201410529820A CN 104236979 A CN104236979 A CN 104236979A
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Abstract
The invention discloses a device capable of simultaneously extracting carbohydrate chains. The device is composed of a support frame, a left corner main body, a right corner main body, a ship type main body, a semipermeable membrane, long hoses and short hoses. The left corner main body and the right corner main body are arranged on the support frame and provided with corresponding holes respectively, and every two corresponding holes are communicated through the corresponding long hose and the corresponding short hose; the upper bottom of the left corner body is provided with the ship type main body, and the ship type main body is provided with holes corresponding to the left corner main body; the semipermeable membrane is arranged between the left corner main body and the ship type main body, and the upper bottom and the lower bottom are provided with sealing gaskets. The device capable of simultaneously extracting the carbohydrate chains is reasonable in structure, practical and convenient, simplifies operations and can process multiple samples of the same variety or different varieties; the column volume of every hole can keep identical, so that components contained in the samples can be separated and collected roughly at the same time, and especially for parallel samples, accidental errors can be eliminated.
Description
Technical field
The present invention relates to a kind of device of Experiment of Analytical Chemistry, specifically a kind of device that simultaneously can extract sugar chain, be especially a kind ofly applicable to the device that sugar chain can be extracted in 96 holes simultaneously.
Background technology
One of key of sugar group research is the acquisition to sugar chain, namely carries out separation and purification to the sugar chain that glycoprotein discharges through enzyme process or chemical method.How obtaining sugar chain is a sugar group Epidemiological Analysis technical difficult problem, and at present, preparation method many employings enzymatic isolation method of sugar chain or Chemical releases method on glycoprotein, this separation and purification just determining sugar chain mainly comprises other small molecular weight impurity of de-albumen, desalination and removing.
The main separation method using bag filter and activated-charcoal column to combine carries out separation and purification to sample at present.Dialysis utilizes solution concentration diffusional effect, by material little for molecular weight as salt ion, oligonucleotide chain etc. penetrate in distilled water outside bag or dislysate, finite concentration can be kept poor, thus remove small-molecule substance by constantly changing water.Activated charcoal belongs to non-polar adsorbent, has certain adsorptive power to sugar chain, is suitable for being separated sugar chain and salt ion.But the method is generally carry out separation and purification operation to simple sample.When carrying out Multi-example or batch sample separation and purification experiment, multiple bag filter and activated-charcoal column need be used to operate or a bag filter and activated-charcoal column repeatable operation simultaneously, existence expends time in, complex operation, workplace are excessive and labour intensity crosses the problems such as high, causes work efficiency too low.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of sugar chain extraction element that simultaneously can realize Multi-example or batch sample separation and purification, this device can obtain the sugar chain of maximum 96 samples release simultaneously.
Technical scheme of the present invention is:
A kind of device that simultaneously can extract sugar chain, comprise support, angle main body of turning left, angle main body of turning right, ship type main body, semi-permeable diaphragm, gasket seal, long soft-pipe and short flexible pipe, described angle main body of turning left is located on support with angle main frame of turning right, described angle main body of turning left is provided with corresponding hole with angle main body of turning right, two corresponding holes are by long soft-pipe and short soft pipe connection, in close main body side, angle of turning left, all long soft-pipes are enclosed with METAL HEATING PROCESS block, and all short hose package are wrapped with metal cooling block; Be provided with ship type main body at described angle main body upper bottom surface of turning left, described ship type main body is provided with the hole corresponding with angle main body of turning left, and is provided with semi-permeable diaphragm turning left between angle main body and ship type main body, is respectively equipped with gasket seal at the upper bottom surface of semi-permeable diaphragm.
Preferably, the upper bottom surface of described angle main body of turning right is higher than the upper bottom surface of angle main body of turning left, to ensure that liquid can be full of flexible pipe and angle main body of turning left smoothly from atmospheric pressure can be utilized during the hole application of sample of angle main body of turning right to ensure, between the liquid level of angle main body of simultaneously guaranteeing to turn left and semi-permeable diaphragm, there is not bubble.Described angle main body of turning left, angle main body of turning right are symmetrical is respectively provided with 96 identical holes of size, and described hole is according to the format permutation of 96 porocyte culture plates.
Described gasket seal is provided with the hole corresponding with angle main body of turning left, and described hole, according to the format permutation of 96 porocyte culture plates, can be connected with 96 holes of angle main body of turning left with the ship type main body contacted up and down.The material of described gasket seal is waterproof sponge, and gasket seal can play a buffer action, the sealing guaranteed ship type main body and turn left between the main body of angle, ensures that the solution No leakage phenomenon in hole occurs.
In each hole of described turn left angle main body and angle main body of turning right, the mouth of pipe of long soft-pipe is higher than the mouth of pipe of short flexible pipe; The bottom of described long soft-pipe is lower than the bottom of short flexible pipe.All long soft-pipes are being enclosed with METAL HEATING PROCESS block near main body side, angle of turning left, and all short flexible pipes are being enclosed with metal cooling block near main body side, angle of turning left.Thus realize cold and hot convection current in the main body of left and right turning, in the hole that in the hole of the angle main body that ensures to turn right, d/d sugar chain transfers to angle main body of turning left and then pass semi-permeable diaphragm by the charcoal absorption in ship type main body.Described long soft-pipe and short flexible pipe are U-shaped flexible pipe.
Four angles of described ship type main body and angle main body of turning left are respectively equipped with mounting hole, and ship type main body and angle main body of turning left are fixed on support by column.Preferably, described mounting hole is processed with internal thread, and described column is processed with external thread, and ship type main body, angle main body of turning left also tightly are fixed on support by the top nut of column, between described nut and ship type main body, be provided with pad.
Described semi-permeable diaphragm is individual layer dialysis membrane.The aperture of semi-permeable diaphragm can experimentally demand regulate, and its molecular cut off MWCO should be less than the molecular weight of the glycoprotein being extracted sugar chain, ensures that glycoprotein cannot pass semi-permeable diaphragm.
Beneficial effect of the present invention:
The present invention is rational in infrastructure, practical and convenient, can the multiple identical or different kind sample of same time-triggered protocol, the column volume in each hole can be consistent, and flow velocity can be kept consistent, make component contained by sample to be separated within the basically identical time and to collect like this, especially can eliminate accidental error for parallel sample.This invention simplifies operation, be applicable to small-molecule substance separation and purification experiment, is the optimal separation tools for purification of sugar chain analysis design mothod during sugar group is learned.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the scheme of installation of hull main body of the present invention and angle main body of turning left.
In figure, 1-support, 2-turns left angle main body, and 3-turns right angle main body, 4-ship type main body, 5-semi-permeable diaphragm, 6-gasket seal, 7-long soft-pipe, the short flexible pipe of 8-, 9-METAL HEATING PROCESS block, 10-metal cooling block, 11-column, 12-nut, 13-pad.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.
As depicted in figs. 1 and 2, a device for sugar chain can be extracted simultaneously, comprise support 1, angle main body 2 of turning left, angle main body 3 of turning right, ship type main body 4, semi-permeable diaphragm 5, gasket seal 6, long soft-pipe 7, short flexible pipe 8, METAL HEATING PROCESS block 9, metal cooling block 10 and column 11; Described angle main body 2 and the angle main body 3 of turning right of turning left is erected on support 1, and the upper bottom surface of angle main body 3 of turning right is higher than the upper bottom surface of angle main body 2 of turning left; Described angle main body 2 of turning left is provided with 96 identical holes of corresponding size with angle main body 3 of turning right, and hole is according to the format permutation of 96 porocyte culture plates; Angle main body 2 of turning left two holes corresponding with angle main body 3 of turning right are communicated with short flexible pipe 8 by long soft-pipe 7, and in each hole, the mouth of pipe of long soft-pipe 7 is higher than the mouth of pipe of short flexible pipe 8, and the bottom of long soft-pipe 7 is lower than the bottom of short flexible pipe 8; In close angle main body 2 side of turning left, all long soft-pipes 7 are enclosed with METAL HEATING PROCESS block 9, and all short flexible pipes 8 are enclosed with metal cooling block 10; Ship type main body 4 is provided with at described angle main body 2 upper bottom surface of turning left, described ship type main body 4 is provided with the hole corresponding with angle main body 2 of turning left, be provided with semi-permeable diaphragm 5 turning left between angle main body 2 and ship type main body 4, be respectively equipped with gasket seal 6 at the upper bottom surface of semi-permeable diaphragm 5, gasket seal 6 is provided with the hole corresponding with angle main body 2 of turning left; Described ship type main body 4 and four angles of angle main body 2 of turning left arrange the female mounting hole of processing respectively, ship type main body 4 and angle main body 2 of turning left to be fixed on support 1 by being processed with externally threaded column 11, the top of column 11 is also tight with nut 12, between described nut 12 and ship type main body 4, be provided with pad 13.
Described semi-permeable diaphragm 5 is individual layer dialysis membrane.The material of described gasket seal 6 is waterproof sponge.
During use, activated charcoal can be loaded as required in the hole of ship type main body 4, and the glycoprotein of sugar chain to be released and corresponding enzyme and suitable damping fluid are joined turn right in the hole of angle main body 3.Sugar chain after enzymolysis is through dialysis membrane 5 under the drive of cold cycling, and by the charcoal absorption of ship type main body 4 li, profit at utmost can change the sugar chain in the main hole of absorption left and right turning in this way.Take out the activated charcoal in ship type main body 4 hole after the completion of reaction, wash out by the sugar chain adsorbed with acetonitrile solution, be spin-dried for rear removal organic solvent can obtain purifying after sugar chain.
Claims (8)
1. one kind can be extracted the device of sugar chain simultaneously, it is characterized in that comprising support, angle main body of turning left, angle main body of turning right, ship type main body, semi-permeable diaphragm, gasket seal, long soft-pipe and short flexible pipe, described angle main body of turning left is located on support with angle main frame of turning right, described angle main body of turning left is provided with corresponding hole with angle main body of turning right, two corresponding holes are by long soft-pipe and short soft pipe connection, in close main body side, angle of turning left, all long soft-pipes are enclosed with METAL HEATING PROCESS block, and all short hose package are wrapped with metal cooling block; Be provided with ship type main body at described angle main body upper bottom surface of turning left, described ship type main body is provided with the hole corresponding with angle main body of turning left, and is provided with semi-permeable diaphragm turning left between angle main body and ship type main body, is respectively equipped with gasket seal at the upper bottom surface of semi-permeable diaphragm.
2. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that the upper bottom surface of upper bottom surface higher than angle main body of turning left of described angle main body of turning right.
3. the device that simultaneously can extract sugar chain according to claim 1, it is characterized in that described angle main body of turning left, angle main body of turning right are symmetrical respectively and be provided with 96 identical holes of size, described hole is according to the format permutation of 96 porocyte culture plates.
4. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described gasket seal is provided with the hole corresponding with angle main body of turning left.
5. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that the mouth of pipe of the mouth of pipe higher than short flexible pipe of long soft-pipe in each hole of described turn left angle main body and angle main body of turning right; The bottom of described long soft-pipe is lower than the bottom of short flexible pipe.
6. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described ship type main body and four angles of angle main body of turning left are respectively equipped with mounting hole, and ship type main body and angle main body of turning left are fixed on support by column.
7. the device that simultaneously can extract sugar chain according to claim 1, it is characterized in that described mounting hole is processed with internal thread, described column is processed with external thread, ship type main body, angle main body of turning left also tightly are fixed on support by the top nut of column, between described nut and ship type main body, be provided with pad.
8. the device that simultaneously can extract sugar chain according to claim 1, is characterized in that described semi-permeable diaphragm is individual layer dialysis membrane; The material of described gasket seal is waterproof sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529820.XA CN104236979B (en) | 2014-10-09 | 2014-10-09 | A kind of device that can simultaneously extract sugar chain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529820.XA CN104236979B (en) | 2014-10-09 | 2014-10-09 | A kind of device that can simultaneously extract sugar chain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104236979A true CN104236979A (en) | 2014-12-24 |
| CN104236979B CN104236979B (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410529820.XA Expired - Fee Related CN104236979B (en) | 2014-10-09 | 2014-10-09 | A kind of device that can simultaneously extract sugar chain |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1890774A (en) * | 2003-10-10 | 2007-01-03 | 蛋白质发现公司 | Methods and devices for concentration and purification of analytes for chemical analysis including matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) |
| CN101759251A (en) * | 2010-02-12 | 2010-06-30 | 湖州富优得膜分离科技有限公司 | Special membrane treatment plant soaking solution process for anti-fouling integrated material and device thereof |
| WO2013047707A1 (en) * | 2011-09-29 | 2013-04-04 | 住友ベークライト株式会社 | Sugar chain refinement device and sugar chain refinement method |
| CN204302069U (en) * | 2014-10-09 | 2015-04-29 | 南京农业大学 | A kind of device that simultaneously can extract sugar chain |
-
2014
- 2014-10-09 CN CN201410529820.XA patent/CN104236979B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1890774A (en) * | 2003-10-10 | 2007-01-03 | 蛋白质发现公司 | Methods and devices for concentration and purification of analytes for chemical analysis including matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) |
| CN101759251A (en) * | 2010-02-12 | 2010-06-30 | 湖州富优得膜分离科技有限公司 | Special membrane treatment plant soaking solution process for anti-fouling integrated material and device thereof |
| WO2013047707A1 (en) * | 2011-09-29 | 2013-04-04 | 住友ベークライト株式会社 | Sugar chain refinement device and sugar chain refinement method |
| CN204302069U (en) * | 2014-10-09 | 2015-04-29 | 南京农业大学 | A kind of device that simultaneously can extract sugar chain |
Non-Patent Citations (2)
| Title |
|---|
| FABIO APARECIDO CORDEIRO .ETC: "Proteomics Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract", 《JOURNAL OF PROTEOME RESEARCH》 * |
| 孙兴权 等: "糖组学研究中糖蛋白糖链结构分析技术", 《化学进展》 * |
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| CN104236979B (en) | 2016-08-17 |
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