CN204241491U - The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain - Google Patents
The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain Download PDFInfo
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- CN204241491U CN204241491U CN201420708887.5U CN201420708887U CN204241491U CN 204241491 U CN204241491 U CN 204241491U CN 201420708887 U CN201420708887 U CN 201420708887U CN 204241491 U CN204241491 U CN 204241491U
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Abstract
The utility model discloses the immunology detection test card that a kind of Medroxyprogesterone and antibody thereof are residual, comprise plastics to get stuck and be packaged in plastics and to get stuck interior test strips, the middle part that described plastics get stuck is provided with well, the both sides of this well are respectively equipped with the first symmetrical view window, second view window and bleeder vent, and be provided with handheld terminal in the position of well, the bottom of described test strips is the supporting layer that PVC board is formed, what be attached to supporting layer central authorities is sample pad, the get stuck position of upper well of this sample pad and plastics is corresponding, the golden labeling antibody pad be closely connected is provided with successively on the left of sample pad, nitrocellulose filter and absorption pad, the gold mark antigen pad be closely connected is provided with successively on the right side of sample pad, nitrocellulose filter and absorption pad.The utility model is simple to operate, can remain simultaneously carry out batch qualitative detection to Medroxyprogesterone and antibody thereof.
Description
Technical field
The utility model belongs to immunochemistry detection technique field, is specifically related to the immunology detection test card that a kind of qualitative detection Medroxyprogesterone and antibody thereof are residual.
Background technology
Medroxyprogesterone acetate (Medroxyprogesterone acetate, MPA), also known as Medroxyprogesterone, medroxyprogesterone acetate, medroxyproges-terone acetate, be a kind of progestogens medicine, be mainly used in the treatment of long-acting contraception, miscarriage, functional amenorrhea, uterine hemorrhage etc. and the palliative treatment of some tumours clinically.In livestock breeding industry, MPA is the same with other protein anabolic hormone medicines, as growth promoter be used for animal getting fat and estrus estrus induction, bring out superfecundation, synchronization of estrus etc., to improve breeding potential and economic benefit.But MPA has toxic and side effect widely, can cause that scrambling is hemorrhage, amenorrhoea, mental depression, insomnia, feel sick, the bad reaction such as mammary swelling, even cause infertility, therefore MPA as feed addictive in animal body constantly accumulation serious threat human health.EU member country forbids to use MPA already in food source property animal feeding management, and specifies that its residual the highest limitation (MRL) in food source property animal product is " must not detect ".Clearly medroxyprogesterone acetate and preparation thereof are classified as forbidden drugs in No. 193 bulletin " veterinary drug of food animal forbidding and other compound inventory " that the Ministry of Agriculture of China issues in April, 2002.
At present, the method for detecting residue of Medroxyprogesterone and derivant thereof mainly contains high performance liquid chromatography (HPLC), GC-MS(gas chromatography-mass spectrography) (GC-MS), and liquid chromatograph mass spectrography (LC-MS) or multistage coupling technique, as LC-MS-MS etc.Physico-chemical tests method, although highly sensitive, needs expensive instrument and equipment, veteran operating personnel, and the extraction and cleaning process of complexity, is unfavorable for applying in production practices.Colloid gold label immunoassay is the novel analytical technology of one developed rapidly in recent years, be characterized in easy fast, cost is low, pollution-free, without the need to training, and all pack are contained in a test strips, are very suitable for Site Detection, have wide market outlook and using value.This problem obtains state natural sciences fund (U1204310) and Henan Province Universities scientific and technical innovation talent plan (14HASTIT026) financial support.
Summary of the invention
The technical matters that the utility model solves there is provided a kind of easy and simple to handle, quick and precisely, highly sensitive, with low cost and good stability, the Medroxyprogesterone of batch detection and the residual immunology detection test card of antibody thereof can be carried out.
The technical solution of the utility model is: the immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain, it is characterized in that comprising plastics to get stuck and be packaged in plastics and to get stuck interior test strips, the middle part that described plastics get stuck is provided with well, the both sides of this well are respectively equipped with the first symmetrical view window, second view window and bleeder vent, and be provided with handheld terminal in the position of well, the bottom of described test strips is the supporting layer that PVC board is formed, what be attached to supporting layer central authorities is sample pad, the get stuck position of upper well of this sample pad and plastics is corresponding, the golden labeling antibody pad be closely connected is provided with successively on the left of sample pad, nitrocellulose filter and absorption pad, the gold mark antigen pad be closely connected is provided with successively on the right side of sample pad, nitrocellulose filter and absorption pad.
The get stuck shape of upper well of plastics described in the utility model is funnel-form elliptical openings, the two ends that plastics get stuck have bleeder vent, and bleeder vent is multiple, in fan-shaped array, and bleeder vent, the first view window, the second view window and well are in the same level that plastics get stuck.
Nitrocellulose filter on the left of sample pad described in the utility model is coated with the detection line T line of medroxyprogesterone acetate-OVA coating antigen printing and the nature controlling line C line of sheep anti-mouse igg solution printing, between the two apart from 5mm, the detection line T line that wherein medroxyprogesterone acetate-OVA coating antigen is printed is positioned at the side near sample pad, and the nature controlling line C line of the detection line T line of medroxyprogesterone acetate-OVA coating antigen printing and the printing of sheep anti-mouse igg solution and plastics get stuck, the position of upper first view window is corresponding.Golden labeling antibody pad on the left of sample pad described in the utility model is perfused with the anti-Medroxyprogesterone monoclonal antibody of colloid gold label.
Nitrocellulose filter on the right side of sample pad described in the utility model is coated with the detection line T line of anti-Medroxyprogesterone monoclonal antibody printing and the nature controlling line C line of sheep anti-mouse igg solution printing, between the two apart from 5mm, the detection line T line that wherein anti-Medroxyprogesterone monoclonal antibody is printed is positioned at the side near sample pad, and the nature controlling line C line of the detection line T line of anti-Medroxyprogesterone monoclonal antibody printing and the printing of sheep anti-mouse igg solution and plastics get stuck, the position of upper second view window is corresponding.Gold mark antigen pad on the right side of sample pad described in the utility model is perfused with the medroxyprogesterone acetate carrier protein of colloid gold label.
The utility model has following beneficial effect:
(1) fast simple, convenient.Without the need to other any instrument or reagent during use quick detection test paper card, only 4-6 in the sample solution dropper instillation test card well after process need be dripped, 3-5 min gets final product observations.
(2) Medroxyprogesterone and antibody thereof can be detected remain simultaneously, result image, directly perceived.When C line on the first view window nitrocellulose filter shows redness " I " trace, represent that Medroxyprogesterone content is positive, when two redness " II " traces appear in C line and T line simultaneously, represent that Medroxyprogesterone content is negative; When C line on the second view window nitrocellulose filter shows redness " I " trace, represent that antibody content is positive, when two redness " II " traces appear in C line and T line simultaneously, represent that antibody content is negative.Result is visual in image, simply accurately, is not easy to occur erroneous judgement.
(3) plastics get stuck on bleeder vent make solution " climbing " smooth and easy, developing time is short.Bleeder vent makes get stuck interior very narrow space and the external world of plastics maintain the circulation of air, ensure that reaction film normally plays capillarity, enable detection thing rapidly displacement to bleeder vent, avoid retardance or the incomplete phenomenon such as swimming, improve colour developing efficiency.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model, and Fig. 2 is the side view of test strips in the utility model.
Drawing illustrates: 1, plastics get stuck, the 2, first view window, the 3, second view window, 4, well, 5, bleeder vent, 6, handheld terminal, 7, supporting layer, 8, sample pad, 9, golden labeling antibody pad, 10, gold mark antigen pad, 11, nitrocellulose filter, 12, nature controlling line C line, 13, the detection line T line printed of medroxyprogesterone acetate-OVA coating antigen, 14, the detection line T line printed of anti-Medroxyprogesterone monoclonal antibody, 15, absorption pad.
Embodiment
Describe embodiment in detail by reference to the accompanying drawings.The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain, comprise plastics and get stuck 1 and be packaged in the test strips that plastics get stuck in 1, described plastics get stuck 1 middle part be provided with well 4, the both sides of this well 4 are respectively equipped with the first symmetrical view window 2, second view window 3 and bleeder vent 5, and be provided with handheld terminal 6 in the position of well 4, the bottom of described test strips is the supporting layer 7 that PVC board is formed, be attached to supporting layer 7 central authorities for sample pad 8, this sample pad 8 and the plastics position of well 4 on 1 of getting stuck is corresponding, the golden labeling antibody pad 9 be closely connected is provided with successively on the left of sample pad 8, nitrocellulose filter 11 and absorption pad 15, wherein nitrocellulose filter 11 is coated with the detection line T line 13 of medroxyprogesterone acetate-OVA coating antigen printing and the nature controlling line C line 12 of sheep anti-mouse igg solution printing, between the two apart from 5mm, and the detection line T line 13 printed of medroxyprogesterone acetate-OVA coating antigen and the nature controlling line C line 12 that sheep anti-mouse igg solution is printed and plastics get stuck, the position of the first view window 2 on 1 is corresponding, gold labeling antibody pad 9 is perfused with the anti-Medroxyprogesterone monoclonal antibody of colloid gold label, the gold mark antigen pad 10 be closely connected is provided with successively on the right side of sample pad 8, nitrocellulose filter 11 and absorption pad 15, wherein nitrocellulose filter 11 is coated with the detection line T line 14 of anti-Medroxyprogesterone monoclonal antibody printing and the nature controlling line C line 12 of sheep anti-mouse igg solution printing, between the two apart from 5mm, and the detection line T line 14 printed of anti-Medroxyprogesterone monoclonal antibody and the nature controlling line C line 12 that sheep anti-mouse igg solution is printed and plastics get stuck, the position of the second view window 3 on 1 is corresponding, gold mark antigen pad 10 is perfused with the medroxyprogesterone acetate carrier protein of colloid gold label.
The plastics described in the utility model shape of well 4 on 1 that gets stuck is funnel-form elliptical openings, described plastics get stuck 1 two ends have bleeder vent 5 in fan-shaped array, and ventilative 5 holes, the first view window 2, second view window 3 and well 4 are in plastics gets stuck in the same level of 1.
Supporting layer 7 described in the utility model coordinates with stabilizing agent and auxiliary material the PVC board made by Corvic.Absorption pad 15 described in the utility model is the pure white filter paper that starting material are made by the plant long fibre that water-intake capacity is extremely strong.Sample pad 8 described in the utility model, golden labeling antibody pad 9 and gold mark antigen pad 10 are made by glass fibre cotton.The width that laminates that sample pad 8 described in the utility model marks antigen pad 10 with golden labeling antibody pad 9 and gold is respectively 1-2mm, and absorption pad 15 is 1-2mm with the width that laminates of nitrocellulose filter 11.
The preparation method of test card described in the utility model:
1) preparation of nitrocellulose filter: nitrocellulose filter is placed on X-only unidirectional specking instrument platform, medroxyprogesterone acetate-OVA coating antigen or anti-Medroxyprogesterone monoclonal antibody are put in A pond, RaMIgG is put in B pond, flatten and compress, after start by antigen and two anti-respectively fixed fires on nitrocellulose filter, form detection line T line and nature controlling line C line.After natural drying at room temperature, immersed 30min in confining liquid (mass concentration is the PBS damping fluid of the BSA of 1%, pH 7.4), after 37 DEG C of oven dry, add drying agent, 4 DEG C of sealings are preserved.
2) preparation of pad: glass fibre cotton is cut into the slice that 4mm is wide, putting into containing mass concentration is the BSA of 5%, and mass concentration is the sucrose of 2%, mass concentration be 0.8% NaCl and mass concentration be the NaN of 0.05%
3pBS treating fluid in 20min, 37 DEG C of constant temperature dryings, then by golden labeling antibody or gold mark antigen perfusion processed good glass fibre cotton on, vacuum freeze-drying 4h, is pad.
3) preparation of sample pad: glass fibre cotton with containing mass concentration be the BSA of 2%, mass concentration is the sucrose of 1%, mass concentration be 0.5% sodium borate and mass concentration be the NaN of 0.1%
3pBS process after, drying for standby, is sample pad.
4) preparation of absorption pad: be that the pure white filter paper cutting machine that starting material make is cut into the wide slice of 4 mm by plant long fibre extremely strong for water-intake capacity, blend compounds film carries out one side closed, drying for standby.
5) preparation of test strips: in back up pad (PVC board), nitrocellulose filter, pad, sample pad and absorption pad etc. are fitted together by certain technique, make the wide test strips of 4mm with CM4000 cutting machine, the closed container putting into band drying agent stores.
6) assembling of test card: in getting stuck by certain technique special plastics test strips be packaged in well, the first view window, the second view window and bleeder vent, be test card of the present utility model.
The using method of test card described in the utility model:
1) sample pre-treatments:
Get the animal tissue sample 5g after homogenizer or bruiser homogenate, add 20ml acetonitrile solution (85 parts of acetonitriles+15 parts of water) mixing, then boiling water bath heating 5min; Mixed liquor constant volume is original volume, and the centrifugal 10min of 3000r/min, gets supernatant 1ml, adds 4ml normal hexane degrease, rotates the centrifugal 10min of mixing 5min, 3000r/min, takes off layer solution and detect for test card.
2) sample drips:
After test card is kept flat, with dropper, sample solution is instilled 4-6 in test card well and drip, after 3-5min, get final product observations.
3) Analysis of test results:
Negative: if when two redness " II " traces appear in nature controlling line C line and detection line T line on the first view window or the second view window nitrocellulose filter, be judged to feminine gender simultaneously.
Positive: if nature controlling line C line shows redness " I " trace on the first view window or the second view window nitrocellulose filter, and detection line T line does not develop the color, and is judged to the positive.
Invalid: if nature controlling line C line does not show red stripes on the first view window or the second view window nitrocellulose filter, then no matter whether detection line T line occurs red trace, and it is invalid that this test card is all judged to.
Above embodiment is only and technological thought of the present utility model is described; protection domain of the present utility model can not be limited with this; every technological thought according to the utility model proposes, any change that technical scheme basis is done, all falls within the utility model protection domain.
Claims (2)
1. the immunology detection test card that a Medroxyprogesterone and antibody thereof are residual, it is characterized in that comprising plastics to get stuck and be packaged in plastics and to get stuck interior test strips, the middle part that described plastics get stuck is provided with well, the both sides of this well are respectively equipped with the first symmetrical view window, second view window and bleeder vent, and be provided with handheld terminal in the position of well, the bottom of described test strips is the supporting layer that PVC board is formed, what be attached to supporting layer central authorities is sample pad, the get stuck position of upper well of this sample pad and plastics is corresponding, the golden labeling antibody pad be closely connected is provided with successively on the left of sample pad, nitrocellulose filter and absorption pad, wherein nitrocellulose filter is coated with the detection line T line of medroxyprogesterone acetate-OVA coating antigen printing and the nature controlling line C line of sheep anti-mouse igg solution printing, between the two apart from 5mm, the detection line T line that medroxyprogesterone acetate-OVA coating antigen is printed is positioned at the side near sample pad, and the detection line T line of medroxyprogesterone acetate-OVA coating antigen printing and the nature controlling line C line that sheep anti-mouse igg solution is printed and plastics get stuck, the position of upper first view window is corresponding, gold labeling antibody pad is perfused with the anti-Medroxyprogesterone monoclonal antibody of colloid gold label, the gold mark antigen pad be closely connected is provided with successively on the right side of sample pad, nitrocellulose filter and absorption pad, wherein nitrocellulose filter is coated with the detection line T line of anti-Medroxyprogesterone monoclonal antibody printing and the nature controlling line C line of sheep anti-mouse igg solution printing, between the two apart from 5mm, the detection line T line that anti-Medroxyprogesterone monoclonal antibody is printed is positioned at the side near sample pad, and the detection line T line of anti-Medroxyprogesterone monoclonal antibody printing and the nature controlling line C line that sheep anti-mouse igg solution is printed and plastics get stuck, the position of upper second view window is corresponding, gold mark antigen pad is perfused with the medroxyprogesterone acetate carrier protein of colloid gold label.
2. the immunology detection test card that Medroxyprogesterone according to claim 1 and antibody thereof are residual, it is characterized in that: the two ends that described plastics get stuck have the bleeder vent in fan-shaped array, and bleeder vent, the first view window, the second view window and well are in the same level that plastics get stuck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420708887.5U CN204241491U (en) | 2014-11-24 | 2014-11-24 | The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420708887.5U CN204241491U (en) | 2014-11-24 | 2014-11-24 | The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain |
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| Publication Number | Publication Date |
|---|---|
| CN204241491U true CN204241491U (en) | 2015-04-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420708887.5U Expired - Fee Related CN204241491U (en) | 2014-11-24 | 2014-11-24 | The immunology detection test card that a kind of Medroxyprogesterone and antibody thereof remain |
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| CN (1) | CN204241491U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424937A (en) * | 2015-10-05 | 2016-03-23 | 华中农业大学 | Progesterone colloidal gold immunochromatography test strip for monkey early pregnancy detection |
-
2014
- 2014-11-24 CN CN201420708887.5U patent/CN204241491U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424937A (en) * | 2015-10-05 | 2016-03-23 | 华中农业大学 | Progesterone colloidal gold immunochromatography test strip for monkey early pregnancy detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150401 Termination date: 20151124 |