CN106405073A - Colloidal-gold detection card for cyhalothrin - Google Patents
Colloidal-gold detection card for cyhalothrin Download PDFInfo
- Publication number
- CN106405073A CN106405073A CN201510465261.5A CN201510465261A CN106405073A CN 106405073 A CN106405073 A CN 106405073A CN 201510465261 A CN201510465261 A CN 201510465261A CN 106405073 A CN106405073 A CN 106405073A
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- China
- Prior art keywords
- detection
- cyfloxylate
- colloidal
- gold
- card
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention provides a colloidal-gold detection card for cyhalothrin. The colloidal-gold detection card is mainly used for rapidly detecting the content of cyhalothrin in food. According to the invention, a liquid sample absorption part, a colloidal-gold marking part, a detection and reaction part and a water absorption part are successively adhered onto the backing of test paper; the detection and reaction part is coated by a strip of antigen 1 for detection, and the strip is used as a detection line; and the detection and reaction part is further coated by a strip of IgG against protein of a second species of animal, and the strip is used as a control line. The rapid detection card has good specificity, can carry out semi-quantitative detection, is usable at an ambient temperature of 4 to 35 DEG C, and is applicable to rapid detection of cyhalothrin residues in plant-derived food by individual farmers, quality supervision departments of food hygiene, customs and the like. The colloidal-gold detection card has the beneficial effects of good specificity, high sensitivity, capacity of realizing semi-quantitative detection, easiness and convenience in operation, etc.
Description
Technical field
Belong to field of detection of food safety and in particular in food the Harmful Residue detection method, particularly cyfloxylate test paper detecting method.
Background technology
Cyfloxylate（English name：Cyfluthrin）There are hundred trees,CyfluthrinDeng another name.Sterling is sticky, partially crystallizableAmberGrease, active ingredient >=90%, no special odor, non-volatile.It is insoluble in water, be slightly soluble inAlcohol,Readily solubleIn ether, ketone,TolueneDengOrganic solvent, rightAlkaliUnstable, stable to acid.
Cyfloxylate desinsection efficiently, has very good effect to many fruit scales mesh larva, also can some subterranean pest-insects of effectively preventing.With tag and stomach poison function based on, no interior suction and fumigation action.Insecticidal spectrum is wide, and effect is rapid, and the lasting period is long.Tool uses some ovicidal activities, and has Repellent effects to some adults.
In maximum residue limits for pesticide table in China's some agricultural byproducts established, the limitation of cyfloxylate must not exceed 0.02mg/kg for cottonseed oil, must not exceed 0.2mg/kg in pomaceous fruits fruit, citrus fruit, leaf vegetables, in apple, must not exceed 0.5mg/kg.
Content of the invention
Present invention aim at providing quick detection to go out the residual of Fluorine in Foods cypermethrin.
The technical scheme is that the detection card of cyfloxylate and its processing method of detection sample.Cyfloxylate detection card be strip flat shell shape detection card inside the shell arrange detector bar, detection card case surface by detection fenestra and well it is characterised in that test-strips by supporting backboard on paste four kinds of original papers form：Nitrocellulose filter is pasted in the middle part of supporting backboard, supporting pastes blotting paper on backboard one end, and the other end pastes sample pad, blotting paper the inner and nitrocellulose filter overlap joint, then there is one section of gold standard pad between sample pad and nitrocellulose filter, by gold standard pad, sample pad is linked together with nitrocellulose filter.It is the mixture containing the second kind albumen and cyfloxylate antibody in gold standard pad, 2 spaced apart colour developing marking bands are laterally had on nitrocellulose filter, article one, be the detection band containing cyfloxylate protein conjugate, another be the second kind animal protein IgG（Conventional for anti-rabbit antibody or anti-mouse antibody）；Test-strips insert detection card inside the shell, and, just to well, nitrocellulose filter is just to detection fenestra for sample pad.
Sample treatment is：Weigh sample 2 ± 0.05 g in the centrifuge tube of 50 mL, add 10 mL ultra-pure waters, smashed with high-speed organization pulverizer under the conditions of 1800 r/min（About 1 min）, take viscous fluid to add 50% trichloroacetic acid 2 mL and dichloromethane 10 mL, 30 min shaken at a high speed on Clothoid type oscillator.Then it is centrifuged 30 min under the conditions of 4 DEG C of 5000 rpm, takes supernatant 10 mL, add methylene chloride 2 mL, vibration mixing 20 min on cyclotron, then it is centrifuged 30 min under the conditions of 4 DEG C of 12000 rpm, supernatant is the extract of medicine.
It is an advantage of the invention that the cyfloxylate containing in sample can be gone out quick detection, and save testing cost, easy to use, detection is quick, and sensitivity is high, and result is accurate.
Brief description
Fig. 1 is that cyfloxylate detects the card shell and test strip arrangement figure in inside the shell.
Fig. 2 is cyfloxylate detection card test strip structural representation.
Fig. 3 is to show trace band schematic diagram on test strip nitrocellulose filter.
Specific embodiment
1. mark the preparation of collaurum
The preparation of colloidal gold solution：First by 1 g gold chloride（HAuCl4）Powder is dissolved in the chlorauric acid solution that 100 mL ultra-pure waters are configured to 1%；1 L ultra-pure water is taken to heat on speciality electric furnace again, the trisodium citrate aqueous solution of 10 mL 1% is added to after seethe with excitement, continue heating after boiling, the chlorauric acid solution of 10 mL 1% is added after 5 minutes, it is again heated to seethe with excitement, wait color to start timing after being changed into claret, after 10 minutes, stop heating, take off rear natural cooling, be settled to 1 L, as colloidal gold solution.
Antibody labeling：Determine 0.1 mol/l K needed for antibody2CO3Ratio, take 100 mL colloidal gold solutions, add the K of this ratio 0.1 mol/L2CO3, mix（About 5 min）, then cyfloxylate and the second kind animal protein are used PBS respectively（0.01 mo1/L, pH7.4）Dissolved dilution, to 2 mg/mL, adds the cyfloxylate antibody of 2 mL 2 mg/mL（Or 2 mL 2 mg/mL the second kind animal protein）, mix（5 min）, it is eventually adding 10% BSA 2 mL, mix（5 min）.Centrifugation stays precipitation three times, and the precipitation of this three times collections is gold mark cyfloxylate antibody.
Colloid gold label part is processed：After cyfloxylate antibody is diluted, by a certain percentage by carrier（As glass fibre, polyester film etc.）It is impregnated with, paving uniformly, is taken out and is put in 37 DEG C of constant temperature ovens baking 8-12 h, i.e. as colloid gold label part after being dried.
2. immunochromatography special polymer compound film preparation
Make transparent backing film with a kind of nitrocellulose filter first, backing film covers one layer strong to protein adhesion, what wetting property was good has the chromatographic film of certain pore size.The effect of backing film is the destruction stoping the organic solvent in adhesive to protein in chromatographic film.
3. the spotting methods of chromatographic film
By certain density sweetness antigen（The conjugates that cyfloxylate is formed with carrier mass）Accurate quantification；With a determining deviation（5 mm）Schedule in horizontal stripe shape point in chromatographic film, for detection.
Cyfloxylate detection card is to arrange test strip 2 in detection card shell 1, accompanying drawing 1 is shown in the setting of the structure of detection card shell 1 and test strip 2, detection card shell 1 is strip flat shell shape shell, long 70 mm, wide 20 mm, thick 5 mm, shell wall thickness 1 mm, it is made up of engineering plastics, detection card shell 1 is formed by upper lid and lower cover two semi join, detection card shell 1 has covered detection fenestra 3 and well 4.Test strip 2 is placed in the lower cover of detection card shell 1.
Test strip 2 is the fillet thin slice of sandwich construction, length of a film 60 mm, wide about 4 mm of piece, and thickness is less than 2.5 mm.Test strip 2 is sandwich construction：Bottom is supporting backboard 5, is polyethylene sheets, thickness about 0.5 mm, long 60 mm, wide 4 mm；Nitrocellulose filter 6, celluloid thickness 0.5 mm, long 20 mm, wide 4 mm are pasted in supporting backboard 5 middle part.Have on nitrocellulose filter 6 two spaced apart be shown displayed across trace band, referring to accompanying drawing 3, in two display traces, one is detection line 10, conjugate containing cyfloxylate, and another is line of reference 11, the IgG containing the second kind animal protein.Supporting pastes blotting paper 9 on backboard 5 one end, the other end pastes sample pad 8, and blotting paper 9 the inner is overlapped with nitrocellulose filter 6, then has one section of gold standard pad 7 between sample pad 8 and nitrocellulose filter 6, by gold standard pad 7, sample pad 8 is linked together with nitrocellulose filter 6, lap width is in 1-2 mm.Sample pad 8 material is water adsorption glass fiber or polyester film.
During using cyfloxylate colloidal-gold detecting-card, first by sample liquid to be detected（After the extract dilution of medicine, it is directly used in detection）The sample pad 8 of cyfloxylate test strip 2 is instilled from well 4, due to rainbow action principle, drive the anti-cyfloxylate monoclonal antibody colloid gold label thing contained by sample liquid to be detected and colloidal gold film 7 to spread to nitrocellulose filter 6 together, in 5-10 minute, observe result.
The key reaction of detection card is immunologic antigen and antibody response, the antibody of the colloid gold label of migration on nitrocellulose filter, on p-wire and containing cyfloxylate protein conjugate, and line of reference contains the IgG reaction of the second kind animal protein, forms brownish red band.If cyfloxylate to be measured is higher than permissible value accordingly in sample, the antibody response first and in gold standard pad after sample addition, the cyfloxylate protein conjugate without being carried with detection band is reacted, thus not developing the color.Mainly there are following 3 kinds of testing results：
1. detection line 10 and line of reference 11 manifest brownish red trace simultaneously, represent that testing result is feminine gender, illustrate not containing cyfloxylate in sample or content is less than permissible value；
2. detection line 10 does not develop the color, and line of reference 11 manifests brownish red trace, represents that testing result is the positive, illustrates that in sample, cyfloxylate content is higher than permissible value；
3. detection line 10 manifests brownish red trace, and line of reference 11 does not develop the color, or detection line 10 and line of reference 11 all do not develop the color, and represents that test strip lost efficacy.
Claims (4)
1. cyfloxylate colloidal-gold detecting-card it is characterised in that：Sample liquid absorption part, colloid gold label part, detection reactive moieties and water absorbent portion are posted on the backing of test paper, the material that colloid gold label is partly labeled is the mixture of the second kind animal protein and cyfloxylate antibody successively；Detection reactive moieties are coated with detection cyfloxylate antigen 1 bar as detection line, and the IgG 1 being simultaneously also coated with anti-second kind animal protein is as line of reference.
2. cyfloxylate colloidal-gold detecting-card according to claim 1 it is characterised in that：Cyfloxylate detection antigen is the conjugates that cyfloxylate is formed with carrier mass.
3. cyfloxylate colloidal-gold detecting-card according to claim 2 it is characterised in that：Described carrier mass, is protein, protein fragments, synthesis polypeptide, semi-synthetic polypeptide or polysaccharide.
4. cyfloxylate colloidal-gold detecting-card according to claim 1 it is characterised in that：The second described kind animal protein is the protein of non-antibody source animal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510465261.5A CN106405073A (en) | 2015-08-03 | 2015-08-03 | Colloidal-gold detection card for cyhalothrin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510465261.5A CN106405073A (en) | 2015-08-03 | 2015-08-03 | Colloidal-gold detection card for cyhalothrin |
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| Publication Number | Publication Date |
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| CN106405073A true CN106405073A (en) | 2017-02-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510465261.5A Pending CN106405073A (en) | 2015-08-03 | 2015-08-03 | Colloidal-gold detection card for cyhalothrin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN109813901A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of immune colloid gold detection card and preparation method thereof detecting ethiprole |
| CN109813900A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chloramphenicol |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509757A (en) * | 2013-07-03 | 2014-01-15 | 江南大学 | Hybridoma cell strain No. 15, and pyrethroid-resistant mass selection monoclonal antibody produced by hybridoma cell strain No. 15 |
| CN103808939A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Spectinomycin colloidal gold detection card |
-
2015
- 2015-08-03 CN CN201510465261.5A patent/CN106405073A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808939A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Spectinomycin colloidal gold detection card |
| CN103509757A (en) * | 2013-07-03 | 2014-01-15 | 江南大学 | Hybridoma cell strain No. 15, and pyrethroid-resistant mass selection monoclonal antibody produced by hybridoma cell strain No. 15 |
Non-Patent Citations (3)
| Title |
|---|
| SALLY K. MAK ET AL.: "Development of a class selective immunoassay for the type II pyrethroid insecticides", 《ANALYTICA CHIMICA ACTA》 * |
| 王东方 等: "拟除虫菊酯类农药通用人工抗原合成及免疫原性鉴定", 《农药》 * |
| 陈秀金: "拟除虫菊酯类农药免疫快速检测方法及其定量构效模型的建立", 《中国博士学位论文全文数据库 农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN109813901A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of immune colloid gold detection card and preparation method thereof detecting ethiprole |
| CN109813900A (en) * | 2017-11-21 | 2019-05-28 | 南京亿特生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chloramphenicol |
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Application publication date: 20170215 |
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