CN104655621B - A kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card - Google Patents
A kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card Download PDFInfo
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- CN104655621B CN104655621B CN201510056439.0A CN201510056439A CN104655621B CN 104655621 B CN104655621 B CN 104655621B CN 201510056439 A CN201510056439 A CN 201510056439A CN 104655621 B CN104655621 B CN 104655621B
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Abstract
The invention discloses a kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card, the upper lid being interlocked including lower cover and with lower cover, on be covered with well and peep hole, the speed that test residues of pesticides are fixed with lower cover surveys paper, speed surveys that paper includes bottom plate and portion pastes and fixed climbs sample film in the soleplate, colour developing pad and adsorptive pads are pasted successively in the one end for climbing sample film, the other end for climbing sample film pastes barrier film and enzyme pad successively, the position of the position correspondence observation colour developing pad of peep hole, the position of the position correspondence enzyme pad of well, detection time is short, it is simple to operate, lateral chromatography causes strong antijamming capability, color developing effect is good, it can be widely applied to the quick detection of organophosphor and carbamate chemicals for agriculture, the present invention is applied to technical field of bioengineering.
Description
Technical field
The present invention relates to technical field of bioengineering, more particularly to a kind of Organophosphorus and carbamate pesticides pesticide residue
Quick measuring card.
Background technology
At present, agricultural chemicals is to ensuring that agricultural high yield good harvest makes a great contribution, but easily remains in environment, particularly eats
Agricultural chemicals and its catabolite in product also bring potential hazard to the mankind, and agricultural chemicals, which enters crops, can cause food pollution, endanger
The health of victimization, it may appear that the symptom such as internal organ necrosis, hardening, immunologic function decline and depauperation.According to FAO (Food and Agriculture Organization of the United Nation)
FAO is counted, if without using agricultural chemicals, whole world agricultural accounts for overall crop yield per loss of the annual caused by disease, worm, crop smothering
37%, year loss be up to 126,000,000,000 dollars.With the food-safe requirement of the mankind and the development of international trade, the world is each
State requires that the project of detection is more and more to agricultural byproducts, because pesticide species are more and more, to improve the detection speed of agricultural chemicals,
Testing cost is reduced, multi-residue analysis turns into the main flow of Pesticide Residues analytical technology.Mainly there are gas-chromatography and matter
Compose GC-MS, liquid chromatogram and mass spectrometric hyphenated technique, Capillary Electrophoresis and mass spectrometry and gas phase, liquid chromatogram and multistage
Mass spectrometric hyphenated technique etc..The most representative method for having a U.S. FDA, German DFG method, S19 methods.
Initial the Detection Technologies of Pesticide Residues is only limitted to chemical method, colorimetric method and bioassary method, and detection method lacks single-minded
Property, sensitivity is not also high.1960s gas chromatography applications are analyzed in agricultural chemicals and medicament residue, substantially increase agricultural chemicals and
The detection level of drug residue.Since 1980s, high performance liquid chromatography starts to be widely used in thermally labile
With the analysis of ionic agricultural chemicals and its metabolin.Although the quantitative accurate, sensitivity of chromatography is high, required equipment is expensive,
Professional is needed to operate, and analysis time is long, is unfavorable for field monitoring.Method for detecting residue coverage rate is narrow, and detection speed is slow,
High expensive not adapting to pursue easy, the quick market demand now.
Organophosphorus and carbamate pesticides class agricultural chemicals is widely used in agricultural production as a kind of efficient pesticides.But it is a large amount of
Many potentially hazardous using that can cause, the sitotoxismus phenomenon especially caused by Organo-phosphorus Pesticide Residues in Vegetables is very tight
Weight.And the quick measuring card of existing fast detecting pesticide residue is existed by artificial folding, react insufficient, detection time compared with
It is long, the problems such as antijamming capability is poor.Therefore, develop it is a kind of it is easier, quickly and accurately detect organophosphorus pesticide
Environmentally safe and people's body the health of quick measuring card have very important meaning.
The content of the invention
To solve the above problems, the present invention provide it is a kind of need to only add sample sample liquid can quick detection complete and observe knot
The Organophosphorus and carbamate pesticides pesticide residue quick measuring card of fruit.
The technical solution adopted for the present invention to solve the technical problems is：
A kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card, including lower cover and the upper lid that is interlocked with lower cover,
On be covered with and the speed of test residues of pesticides is fixed with well and peep hole, lower cover surveys paper, speed, which surveys paper, includes the bottom plate and bottom of at
That fixation is pasted in the middle part of plate climbs sample film, and colour developing pad and adsorptive pads are pasted in the one end for climbing sample film successively, climb the other end of sample film successively
Paste in the position of colour developing pad in barrier film and enzyme pad, the position correspondence observation lower cover of peep hole, the position correspondence lower cover of well
The position of enzyme pad, climbs sample film and contains chromogenic substrate.
It is further used as being provided around speed survey paper surrounding on the improvement of technical solution of the present invention, lower lid and determines as speed survey paper
Some convex tendons and/or some salient points of position, upper lid both sides are respectively equipped with positioning bar, positioning bar interlock embedded each convex tendon and/or
In person's salient point and the cavity of lower cover housing formation, lower lid is provided with some holes for being used to position, and upper lid, which is provided with, fastens lower cover simultaneously
The stud of embedded hole.
The improvement of technical solution of the present invention is further used as, the depth of positioning bar insertion lower cover is 0.5mm~1.5mm, convex
The depth for following closely embedded hole is 0.5mm~1.5mm.
The improvement of technical solution of the present invention is further used as, upper lid one end is provided with a boss, and well is located in boss, plus
Sample hole entrance rounding.
The improvement of technical solution of the present invention is further used as, enzyme pad contains cholinesterase.
The improvement of technical solution of the present invention is further used as, amount of the enzyme pad containing cholinesterase is 0.5U~2U, the length of enzyme pad
For 18 mm~22mm and a width of 4mm~6mm.
The improvement of technical solution of the present invention is further used as, chromogenic substrate is indophenols acetic acid esters.
Be further used as the improvement of technical solution of the present invention, climb the amount of sample film acetic acid esters containing indophenols for 0.3 mg~
3.0mg, climbs a length of 9 mm~11mm and a width of 4mm~6mm of sample film.
The improvement of technical solution of the present invention is further used as, colour developing pad is filter paper or synthetic cellulose paper, and bottom plate is band
The PVC paper of viscosity.
The improvement of technical solution of the present invention is further used as, a length of 6 mm~8mm and a width of 4mm~6mm of the pad that develops the color inhale
The a length of 18 mm~22mm and a width of 4mm~6mm of water cushion, a length of 2 mm~4mm and a width of 4mm~6mm of barrier film, well
A length of 4mm~6mm and a width of 2mm~4mm, a length of 3mm~5mm and a width of 3mm~5mm of peep hole.
Beneficial effects of the present invention：This Organophosphorus and carbamate pesticides pesticide residue quick measuring card middling speed survey paper, which is provided with, climbs
Sample film, colour developing pad, adsorptive pads, barrier film and enzyme pad so that added in well after sample liquid, the enzyme r e lease in enzyme pad is in aqueous phase
In, acted on and climbed on sample film by lateral chromatography, contacted and react, reaction product continues to chromatograph by colour developing pad capture with aqueous phase
Accumulation, the more early haptoreaction of enzyme-to-substrate completes only to need 7min so that being loaded onto detection, or even shorter, and detection time is short,
Cost is low；Enzyme pad can play a part of filtering absorption to the pigment and other impurities in sample liquid, so that quick measuring card
Strong antijamming capability, color developing effect is good；The product reacted at first in course of reaction is accumulated on colour developing pad, with lateral chromatography
Effect may proceed to move to adsorptive pads, and the product reacted afterwards continues to supplement accumulation on colour developing pad, if there is agricultural chemicals in sample liquid
The generation object amount that residual is reacted after causing is reduced, and the color of colour developing pad will weaken, or even bleach, and sensitivity is high.
The pesticide residue fast detection card of the present invention can be widely applied to the quick inspection of organophosphor and carbamate chemicals for agriculture
Survey, the detection sample such as veterinary antibiotics can be loaded detection by simple process, only need a step, and it is that may wait for add sample sample liquid
Detection completes observation result, so as to avoid artificial folding, the problems such as reacting insufficient is simple to operate, convenient and swift.
Brief description of the drawings
The invention will be further described below in conjunction with the accompanying drawings：
Fig. 1 is that speed described in the embodiment of the present invention surveys the structural representation that paper is positioned over lower cover；
Fig. 2 is lower cover overall structure diagram described in the embodiment of the present invention；
Fig. 3 is upper lid overall structure diagram described in the embodiment of the present invention；
Fig. 4 is upper lid sectional view described in the embodiment of the present invention；
Fig. 5 is that speed surveys paper overall structure diagram described in the embodiment of the present invention；
Fig. 6 is that speed surveys paper side view described in the embodiment of the present invention.
Embodiment
The technique effect of the design of the present invention, concrete structure and generation is carried out below with reference to embodiment and accompanying drawing clear
Chu, it is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of embodiment, rather than whole embodiments, based on embodiments of the invention, those skilled in the art is not paying
The other embodiment obtained on the premise of creative work, belongs to the scope of protection of the invention.
1~Fig. 6 of reference picture, it is of the invention for a kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card, including lower cover 2
And the upper lid 3 being interlocked with lower cover 2, upper lid 3 is provided with well 34 and peep hole 33, is fixed with test residues of pesticides in lower cover 2
Speed survey paper 1, speed survey paper 1 include bottom plate 11 and pasted at the middle part of bottom plate 11 it is fixed climb sample film 14, climb one end of sample film 14 according to
Secondary to paste colour developing pad 15 and adsorptive pads 16, the other end for climbing sample film 14 pastes barrier film 13 and enzyme pad 12, the position of peep hole 33 successively
The position of correspondence observation colour developing pad 15 is put, the position of the position correspondence enzyme pad 12 of well 34 climbs sample film 14 and contains chromogenic substrate.
This Organophosphorus and carbamate pesticides pesticide residue quick measuring card middling speed surveys paper provided with sample film 14 is climbed, the pad 15 that develops the color,
Adsorptive pads 16, barrier film 13 and enzyme pad 12 so that in well 34 add sample liquid after, the enzyme r e lease in enzyme pad 12 in aqueous phase,
Acted on and climbed on sample film 14 by lateral chromatography, contacted and react, reactant continues to chromatograph with aqueous phase captures tired by colour developing pad 15
Product, the more early haptoreaction of enzyme-to-substrate completes only to need 7min, detection time is short, and cost is low, enzyme pad so that being loaded onto detection
Pigment and other impurities in 12 pairs of sample liquids can play a part of filtering absorption, so that the antijamming capability of quick measuring card
By force, color developing effect is good, and the product reacted at first in course of reaction is accumulated on colour developing pad 15, as lateral chromatography effect can be after
Continue and moved to adsorptive pads, the product reacted afterwards continues to supplement accumulation on colour developing pad 15, if there are residues of pesticides to make in sample liquid
The generation object amount reacted after obtaining is reduced, and the color of colour developing pad 15 will weaken, or even bleach, and sensitivity is high.
The pesticide residue fast detection card of the present invention can be widely applied to the quick inspection of organophosphor and carbamate chemicals for agriculture
Survey, the detection sample such as veterinary antibiotics can be loaded detection by simple process, only need a step, and it is that may wait for add sample sample liquid
Detection completes observation result, so as to avoid artificial folding, the problems such as reacting insufficient is simple to operate, convenient and swift.
As the preferred embodiment of the present invention, speed is provided around on lower cover 2 and surveys the surrounding of paper 1 and is positioned as speed survey paper 1
Some convex tendons and/or some salient points, the upper both sides of lid 3 are respectively equipped with positioning bar 32, positioning bar 32 staggeredly embedded each convex tendon and/
Or in salient point and the cavity of the shell of lower cover 2 formation, lower cover 2 is provided with some holes 21 for being used to position, and upper lid 3, which is provided with, to be fastened
The stud 31 of lower cover 2 and embedded hole 21.
As the preferred embodiment of the present invention, the depth of the insertion lower cover 2 of positioning bar 32 is 0.5mm~1.5mm, stud
The depth of 31 embedded holes 21 is 0.5mm~1.5mm.
When positioning bar 32 and stud 31 are embedded in lower cover 1mm, the fastening effect reached is optimal.
As the preferred embodiment of the present invention, upper one end of lid 3 is provided with a boss, and well 34 is in boss, sample-adding
The entrance rounding of hole 34.
As the preferred embodiment of the present invention, enzyme pad 12 contains cholinesterase.
As the preferred embodiment of the present invention, amount of the enzyme pad 12 containing cholinesterase is 0.5U~2U, enzyme pad 12 it is a length of
18 mm~22mm and a width of 4mm~6mm.
The optimal size of enzyme pad 12 is：Long 20mm × wide 5mm, when the amount of the cholinesterase of contained fixation is 0.5U~2U
When, the effect reached is optimal.
As the preferred embodiment of the present invention, chromogenic substrate is indophenols acetic acid esters.
As the preferred embodiment of the present invention, the amount for climbing the acetic acid esters containing indophenols of sample film 14 is 0.3 mg~3.0mg, is climbed
The a length of 9 mm~11mm and a width of 4mm~6mm of sample film 14.
The optimal size for climbing sample film is：Long 10mm × wide 5mm, the amount of the indophenols acetic acid esters of contained fixation is：0.3 mg~
During 3.0mg, the effect reached is optimal.
As the preferred embodiment of the present invention, colour developing pad 15 is filter paper or synthetic cellulose paper, and bottom plate 11 is that band is viscous
The PVC paper of property.
It is used as the preferred embodiment of the present invention, a length of 6 mm~8mm and a width of 4mm~6mm of the pad 15 that develops the color, adsorptive pads
16 a length of 18 mm~22mm and a width of 4mm~6mm, a length of 2 mm~4mm and a width of 4mm~6mm of barrier film 13, well
34 a length of 4mm~6mm and a width of 2mm~4mm, a length of 3mm~5mm and a width of 3mm~5mm of peep hole 33.
Colour developing pad 15 optimal size be：Long 7mm × wide 5mm, material is selected from：Filter paper, synthetic cellulose paper；Adsorptive pads 16
Optimal size be：Long 20mm × wide 5mm；The optimal size of barrier film 13 is：During long 3mm × wide 5mm, the effect reached is most
It is good.The optimal size of well 34 is：Long 5mm × wide 3mm, well 34 is relative with enzyme pad 12, the optimal size of peep hole 33
For：Long 4mm × wide 4mm, peep hole 33 is relative with colour developing pad 15.
The preferred embodiment of the present invention 1：Pasted on bottom plate 11 be fixed with indophenols acetic acid esters climb sample film 14, climb the size of sample film 14
For long 10mm × wide 5mm, the amount for being fixed with indophenols acetic acid esters is 0.3mg；Climb the one end of sample film 14 and paste colour developing pad 15 and water suction
Pad 16, colour developing pad 15 material is synthetic cellulose paper, and size is long 7mm × wide 5mm, and the size of adsorptive pads 16 is long 20mm × wide
5mm, climbs the other end of sample film 14 and is followed by pasting barrier film 13 successively and is fixed with the enzyme pad 12 of cholinesterase.The size of barrier film 13
For long 3mm × wide 5mm, the size of enzyme pad 12 is long 20mm × wide 5mm, and the amount of contained fixed cholinesterase is 0.5U.Speed survey paper 1 is put
In in the groove of lower plastic cover 2, bottom plate 11 is PVC bottom plate paper.
The step of detecting residues of pesticides is as follows：
Step 1: preparing blank sample liquid, blank sample liquid selects pure water, by detector horizontal positioned, toward well
0.1mL pure water is added in 34,7min is reacted under the conditions of 37 DEG C, observation colour developing result observes navy blue, table by peep hole 33
Show non agricultural chemical residuum in sample liquid.
Step 2: preparing agricultural chemicals mark product, 0.5mg/kg flolimats, by detector horizontal positioned, add into well 34
Enter and 7min is reacted under the conditions of 0.1mL 0.5mg/kg flolimats, 37 DEG C, observation colour developing result observes nothing by peep hole 33
Color, represents there are residues of pesticides in sample liquid.
The preferred embodiment of the present invention 2：The amount of fixed cholinesterase is 2U contained by enzyme pad 12, climbs what is fixed contained by sample film 14
The amount of indophenols acetic acid esters is 3.0mg, and the material of barrier film 13 is filter paper.
The step of detecting residues of pesticides is as follows：
Step 1: blank sample liquid selects pure water, by detector horizontal positioned, 0.1mL is added into well 34 pure
Water purification, 7min is reacted under the conditions of 37 DEG C, and observation colour developing result is observed navy blue by peep hole 33, represented in sample liquid without agriculture
Medicine is remained.
Step 2: preparing analog sample, analog sample is mark-on metrifonate to 0.2mg/kg pakchoi sample, vegetables mould
Intend sample process step be：Take 5g fresh weight vegetable samples to be cut into the fragment of about 1cm × 1cm or so sizes, be placed in specimen cup,
10 ml pure water are added afterwards, are shaken 50 times, stand 2min, supernatant is prepare liquid, and prepare liquid is greenish liquid.
Step 3: by detector horizontal positioned, greenish liquid, 37 DEG C in 0.1mL step 2 are added into well 34
Under the conditions of react 7min, observation colour developing result is observed colourless by peep hole 33, represents there are residues of pesticides in sample liquid.
By above-mentioned analog sample detection operate, using various concentrations Organophosphorus and carbamate pesticides class agricultural chemicals mark-on in
Pakchoi sample, detects the detection sensitivity of analog sample, and testing result is as shown in the table：
Certainly, the invention is not limited to above-mentioned embodiment, and those skilled in the art are without prejudice to originally
Equivalent variations or replacement can be also made on the premise of spirit, these equivalent modifications or replacement are all contained in the application right
It is required that in limited range.
Claims (9)
1. a kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card, it is characterised in that：Including lower cover (2) and with it is described under
The upper lid (3) that lid (2) is interlocked, the upper lid (3) is provided with fixed in well (34) and peep hole (33), the lower cover (2)
The speed for having test residues of pesticides surveys paper (1), and the speed, which surveys paper (1), to be included bottom plate (11) and paste solid in the middle part of the bottom plate (11)
Fixed climbs sample film (14), and colour developing pad (15) and adsorptive pads (16) are pasted in described one end for climbing sample film (14) successively, described to climb sample film
(14) the other end is pasted in barrier film (13) and enzyme pad (12), the position correspondence observation lower cover (2) of the peep hole (33) successively
The position of colour developing pad (15), the position of position correspondence lower cover (2) interior enzyme pad (12) of the well (34) is described to climb sample film
(14) chromogenic substrate is contained, the chromogenic substrate is to be provided around speed on indophenols acetic acid esters, the lower cover (2) to survey paper (1) surrounding
And some convex tendons and/or some salient points of paper (1) positioning are surveyed as speed, described upper lid (3) both sides are respectively equipped with positioning bar
(32), the positioning bar (32), which is interlocked, is embedded in the cavity that each convex tendon and/or salient point are formed with lower cover (2) shell, institute
Lower cover (2) is stated provided with some holes (21) for being used to position, the upper lid (3), which is provided with, fastens lower cover (2) and embedded hole (21)
Stud (31).
2. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1, it is characterised in that：The positioning
The depth of the embedded lower cover (2) of muscle (32) is 0.5mm~1.5mm, the depth of stud (31) embedded hole (21) for 0.5mm~
1.5mm。
3. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1 or claim 2, it is characterised in that：It is described
Upper lid (3) one end is provided with a boss, and the well (34) is in boss, well (34) the entrance rounding.
4. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1, it is characterised in that：The enzyme pad
(12) cholinesterase is contained.
5. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 4, it is characterised in that：The enzyme pad
(12) amount containing cholinesterase is 0.5U~2U, a length of 18mm~22mm and a width of 4mm~6mm of the enzyme pad (12).
6. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1, it is characterised in that：It is described to climb sample
The amount of film (14) acetic acid esters containing indophenols is 0.3mg~3.0mg.
7. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 6, it is characterised in that：It is described to climb sample
A length of 9mm~the 11mm and a width of 4mm~6mm of film (14).
8. Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1, it is characterised in that：The colour developing
It is filter paper or synthetic cellulose paper to pad (15), and the bottom plate (11) is adhesive PVC paper.
9. the Organophosphorus and carbamate pesticides pesticide residue quick measuring card according to claim 1 or 7, it is characterised in that：It is described
A length of 6mm~the 8mm and a width of 4mm~6mm of colour developing pad (15), a length of 18mm~22mm of the adsorptive pads (16) and a width of
4mm~6mm, a length of 2mm~4mm and a width of 4mm~6mm of the barrier film (13), a length of 4mm of the well (34)~
6mm and a width of 2mm~4mm, a length of 3mm~5mm and a width of 3mm~5mm of the peep hole (33).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510056439.0A CN104655621B (en) | 2015-02-03 | 2015-02-03 | A kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510056439.0A CN104655621B (en) | 2015-02-03 | 2015-02-03 | A kind of Organophosphorus and carbamate pesticides pesticide residue quick measuring card |
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| CN104655621A CN104655621A (en) | 2015-05-27 |
| CN104655621B true CN104655621B (en) | 2017-09-12 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928929A (en) * | 2016-04-20 | 2016-09-07 | 郑州大学 | Rapid detection point card for pesticide residues and method for detecting content of pesticide residues by using detection point card |
| CN105675606A (en) * | 2016-04-21 | 2016-06-15 | 湖北久华食安生物技术有限公司 | Quick detection card for pesticide residues |
| CN106442660A (en) * | 2016-10-31 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Quick detection card for organic phosphorus pesticide and manufacturing method thereof |
| CN106404772A (en) * | 2016-11-03 | 2017-02-15 | 百奥森（江苏）食品安全科技有限公司 | Reagent card for comprehensive detection on food |
| CN106568890A (en) * | 2016-11-07 | 2017-04-19 | 吴文华 | Portable pesticide residue detection device |
| CN106501250B (en) * | 2016-11-08 | 2020-03-10 | 郑州贝贝生物科技有限公司 | Rapid detection test strip and method for detecting organophosphorus and carbamate pesticide residues by using same |
| CN107478648B (en) * | 2017-08-25 | 2019-11-15 | 东莞市恒度检测科技有限公司 | A kind of pesticide residue fast detection card red and preparation method thereof |
| CN109580608A (en) * | 2018-12-17 | 2019-04-05 | 鲁东大学 | A kind of Rapid pesticide residue detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TW200743799A (en) * | 2006-05-26 | 2007-12-01 | Apex Biotechnology Corp | Methods and apparatus for enzyme inhibitors analysis |
| CN101319997A (en) * | 2007-06-08 | 2008-12-10 | 广州绿洲生化科技有限公司 | Card suitable for fast detecting pesticide residue in tea and detection method thereof |
| CN101693916B (en) * | 2009-11-05 | 2011-08-31 | 上海交通大学 | Card for fast testing residual pesticide |
| CN203414413U (en) * | 2013-08-15 | 2014-01-29 | 北京六角体科技发展有限公司 | Pesticide residue detecting percolation card |
| CN203519615U (en) * | 2013-10-15 | 2014-04-02 | 杭州迪恩科技有限公司 | Detection card for detecting beta-stimulants |
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Address after: 510663 Guangdong Guangzhou high tech Industrial Development Zone, Science City, open source road 11, A2 third. Patentee after: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. Address before: 510663 room 901, 7 science and Technology Street, science and technology Avenue, Guangzhou high tech Industrial Development Zone, Guangdong Patentee before: Guangzhou Oasis Biochemistry Technology Co.,Ltd. |
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Effective date of registration: 20180910 Address after: 510663 Guangdong Guangzhou high tech Industrial Development Zone, Science City, open source road 11, A2 third. Co-patentee after: Guangdong Zhong Da Da Detection Technology Co., Ltd. Patentee after: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. Address before: Guangzhou high tech Industrial Development Zone, Science City, open source road, No. 11, A2 building, third story. Patentee before: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. |
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