CN106442660A - Quick detection card for organic phosphorus pesticide and manufacturing method thereof - Google Patents
Quick detection card for organic phosphorus pesticide and manufacturing method thereof Download PDFInfo
- Publication number
- CN106442660A CN106442660A CN201610926837.8A CN201610926837A CN106442660A CN 106442660 A CN106442660 A CN 106442660A CN 201610926837 A CN201610926837 A CN 201610926837A CN 106442660 A CN106442660 A CN 106442660A
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- detection
- paper
- electrode
- lower cover
- golden finger
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Abstract
The invention discloses a quick detection card for an organic phosphorus pesticide. The quick detection card comprises a lower cover, detection paper and an upper cover, wherein the upper cover is buckled on the lower cover, the upper cover and the lower cover are matched with each other to form a shell, and the detection paper is fixed in the shell; a hydrophobic layer is selectively coated on the surface of the detection paper, a sampling area, a sample feeding channel and a detection area are formed on the area on the surface of the detection paper uncoated with the hydrophobic layer, the sampling area is communicated with the detection area through the sample feeding channel, and a sampling port is formed in the upper cover; and a reference electrode, a work electrode and a counter electrode are also arranged on the detection paper, the detection ends of the reference electrode, the work electrode and the counter electrode are all arranged in the detection area, a golden finger is also arranged at the lower end of the detection paper, the output ends of the reference electrode, the work electrode and the counter electrode are respectively connected with the golden finger, and the golden finger is corresponding to a data port of a detection device. The invention also discloses a manufacturing method for the quick detection card for the organic phosphorus pesticide. The detection card has the advantages of convenience in use, simple operation and high detection speed.
Description
Technical field
The present invention relates to Pesticides Testing technical field, specifically a kind of organophosphorus pesticide rapid detection card and its making side
Method.
Background technology
At present, pesticide is made a great contribution to guaranteeing agricultural high yield good harvest, but easily remains in environment, is particularly eaten
Pesticide and its catabolite in product also gives people class and brings potential hazard, and pesticide enters crops and can cause food pollution, danger
The health of victimization, it may appear that internal organs necrosis, hardening, the symptom such as immunologic function degression and dysplasia.According to FAO (Food and Agriculture Organization of the United Nation)
FAO is counted, if not using pesticide, whole world agricultural is per annual because the loss that disease, worm, crop smothering are caused accounts for overall crop yield
37%, year up to 126,000,000,000 dollars of loss.With mankind's requirement safe to food and the development of international trade, the world is each
To agricultural byproducts, state requires that the project of detection is more and more, as pesticide species are more and more, be the detection speed for improving pesticide,
Reduce testing cost, multi-residue analysis have become the main flow of Pesticide Residues analytical technology.Mainly there is gas chromatogram with matter
Spectrum multiple techniques, liquid chromatograph and mass spectrometric hyphenated technique, capillary electrophoresis and mass spectrometry and gas phase, liquid chromatograph with multistage
Mass spectrometric hyphenated technique etc..The most representative method for having U.S. FDA, the method for German DFG, S19 method.However, these inspections
Survey method generally needs valuable instrument and equipment, and can only carry out in laboratory environment.At present, by the vegetable of national standard
The quick detection of organophosphorus pesticide residual quantity, widely used residual toxicity quick measuring card is to utilize sensitivity high to organophosphorus insecticide
Acetylcholine esterase and the quick measuring card made of developer, not easy to maintain, operation needs manually to pinch pressure with handss, easily causes to react and does not fill
Point, and capacity of resisting disturbance is poor, increased randomness and uncertainty.
Content of the invention
It is an object of the invention to provide a kind of organophosphorus pesticide rapid detection card and preparation method thereof, to solve the above-mentioned back of the body
The problem for proposing in scape technology.
For achieving the above object, the present invention provides following technical scheme：
A kind of organophosphorus pesticide rapid detection card, including lower cover, detection paper and Shang Gai；The upper lid is fastened on down and covers, and on
Lid cooperatively forms shell with lower cover, and detection paper is fixed on inside the shell；Detection paper surface Selective coating has hydrophobic layer, and examines
The region for surveying the uncoated hydrophobic layer in paper surface forms sample application zone, sample presentation passage and detection zone, sample application zone and detection zone interval setting
On detection paper, and it is connected by sample presentation passage between sample application zone and detection zone, above covers and be provided with and sample-adding zone position phase
Corresponding adding mouth；Reaction enzymes region bar is additionally provided with the sample presentation passage, is coated with organophosphors water in the bar of reaction enzymes region
Solution enzyme layer；Reference electrode, working electrode is additionally provided with the detection paper and to electrode, reference electrode, working electrode and to electricity
The test side of pole is arranged in detection zone；The detection paper lower end is additionally provided with golden finger, reference electrode, working electrode and right
The outfan of electrode is respectively connecting to golden finger, and golden finger is corresponding with the FPDP of testing equipment.
As the further scheme of the present invention：Reaction enzymes region bar has three, and the length along sample presentation passage
Direction uniform intervals distribution.
As the present invention further scheme：The reference electrode and it is separately positioned on the two of working electrode to electrode
Side, and reference electrode and to the top of electrode all around the top of working electrode around forming arc-shaped.
As the present invention further scheme：The testing equipment is hand-held testing equipment.
As the present invention further scheme：The golden finger lower end coordinates formed shell through upper lid and lower cover,
And extend to the external world.
As the present invention further scheme：Also include the end cap for protecting golden finger, end cap be set in lid and
Lower cover coordinates the lower end of formed shell, and end cap golden finger is located at interior.
As the present invention further scheme：Cover under described and blocked hole is provided with, above cover and be provided with and blocked hole
Corresponding clip-connecting rod, is fastened with the cooperation of blocked hole by clip-connecting rod between upper lid and lower cover and is connected.
As the present invention further scheme：The blocked hole has four, is separately positioned at the corner of lower cover.
As the present invention further scheme：The positioning right angle muscle being provided with the lower cover for fixed test paper,
Detection paper is embedded in the locating slot constituted by positioning right angle muscle, and positioning right angle muscle is by upper positioning right angle muscle and lower positioning right angle muscle
Composition, upper positioning right angle muscle has two, and is separately positioned on detection paper upper end both sides, and lower positioning right angle muscle has two, point
It is not arranged on detection paper lower end both sides, and detection paper and is additionally provided with the locating notch for matching with lower positioning right angle muscle；Described
It is provided with upper lid for the limit rib spacing to detecting paper, limit rib has two, and is separately positioned on two in the middle part of detection paper
Side.
The manufacture method of described organophosphorus pesticide rapid detection card, step is as follows：
1）Lower cover, upper lid and end cap are made by being molded, standby；
2）By way of wax spray is printed, on the filter paper base material of detection paper, print hydrophobic layer, and the region shape in non-wax spray
Become sample application zone, sample presentation passage and detection zone, obtain filter paper after printing；
3）Filter paper after printing is put in baking oven, at 130-135 DEG C, 8-10min is processed, obtain first drying filter paper；
4）Using screen printing technique in the surface printing reference electrode of first drying filter paper, working electrode and to electrode, one
Golden finger is fixed in secondary drying filter paper lower end, and makes reference electrode, working electrode and electrode is all connected with golden finger, obtains inspection
Survey paper semi-finished product；
5）Organophosphor hydrolytic enzyme is coated in the reaction enzymes region bar of detection paper semi-finished product, after drying naturally, with detection paper half
Organophosphor hydrolytic enzyme layer is formed in the reaction enzymes region bar of finished product, obtain detection paper product；
6）Detection paper product is embedded in lower cover, and upper lid is fastened on down and covers；
7）End cap is enclosed within the overall bottom constituted by lower cover and the conjunction of upper cover buckle, golden finger is located at interior.
Compared with prior art, the invention has the beneficial effects as follows：
1st, organophosphorus pesticide rapid detection card of the present invention is easy to use, simple to operate, coordinates hand-held testing equipment, can be directly perceived
Detect organophosphorus pesticide content in detected sample, detection speed is fast, and the outdoor that can realize organophosphorus pesticide content is fast
Speed detection；
2nd, organophosphorus pesticide rapid detection card of the present invention is rational in infrastructure, compared with common papery detection card, is hardly damaged, it is easy to
Storage, and the interference in the external world can be resisted to a certain extent, advantageously ensure that the accuracy of testing result.
Description of the drawings
Fig. 1 is the structural representation of lower cover and detection paper in organophosphorus pesticide rapid detection card.
Fig. 2 is the structural representation for detecting paper in organophosphorus pesticide rapid detection card.
Fig. 3 is the structural representation of upper lid in organophosphorus pesticide rapid detection card.
Fig. 4 is the overall structure diagram of organophosphorus pesticide rapid detection card.
In figure：1- lower cover, 11- blocked hole, 12- positioning right angle muscle, 2- detection paper, 21- hydrophobic layer, 22- sample application zone, 23-
Sample presentation passage, 24- reaction enzymes region bar, 25- reference electrode, 26- working electrode, 27- are to electrode, 28- detection zone, 29- positioning
Lid, 31- clip-connecting rod, 32- adding mouth, 33- limit rib, 4- end cap on breach, 210- golden finger, 3-.
Specific embodiment
With reference to specific embodiment, technical scheme is described in more detail.
Fig. 1-4 are referred to, a kind of organophosphorus pesticide rapid detection card, including lower cover 1, detection paper 2 and upper lid 3；On described
Lid 3 is fastened on lower cover 1, and upper lid 3 cooperatively forms shell with lower cover 1, and detection paper 2 is fixed on inside the shell, by lower cover 1 and upper
The shell formed by lid 3 is protected to detecting paper 2, it is to avoid the damage of detection paper 2, and detection paper 2 is placed in inside the shell, can
The interference in the external world is resisted to a certain extent；The detection 2 surface Selective coating of paper has hydrophobic layer 21, and detects 2 surface of paper
The region of uncoated hydrophobic layer 21 forms sample application zone 22, sample presentation passage 23 and detection zone 28, and sample application zone 22 and detection zone 28 are spaced
Be arranged on detection paper 2, and be connected by sample presentation passage 23 between sample application zone 22 and detection zone 28, upper cover be provided with 3 with
The corresponding adding mouth 32 in 22 position of sample application zone, sample drops to sample application zone 22, the sample in sample application zone 22 by adding mouth 32
Gradually infiltrated in detection zone 28 by sample presentation passage 23；Reaction enzymes region bar 24 is additionally provided with the sample presentation passage 23, instead
Answer in enzyme region bar 24 and organophosphor hydrolytic enzyme layer is coated with, sample, can be through reaction enzymes during sample presentation passage 23 is flowed through
Region bar 24, is fully contacted with organophosphor hydrolytic enzyme layer so as to sample, and is mixed with the organophosphor hydrolytic enzyme for carrying, when reaching inspection
When surveying area 28, sample is mixed with organophosphor hydrolytic enzyme fully, and specific reaction occurs, to be conducive to detection, reaction enzymes
The quantity of region bar 24 is without restriction, in the present embodiment, it is preferred that reaction enzymes region bar 24 has three, and along giving
The length direction uniform intervals distribution of sample passage 23；
Reference electrode 25, working electrode 26 is additionally provided with and to electrode 27, reference electrode 25, working electrode on the detection paper 2
26 and the test side to electrode 27 be arranged in detection zone 28, reference electrode 25, working electrode 26 and to 27 phase interworking of electrode
Close, for detecting to the sample in detection zone 28, reference electrode 25, working electrode 26 and to the position relationship of electrode 27 not
Plus limit, in the present embodiment, it is preferred that the reference electrode 25 and be separately positioned on the both sides of working electrode 26 to electrode 27,
And reference electrode 25 and to the top of electrode 27 all around the top of working electrode 26 around forming arc-shaped；The detection paper 2
Lower end is additionally provided with golden finger 210, reference electrode 25, working electrode 26 and is respectively connecting to golden finger to the outfan of electrode 27
210, and golden finger 210 is corresponding with the FPDP of testing equipment, testing equipment be with reference electrode 25, working electrode 26 and
The electrode type testing equipment corresponding to electrode 27, the electrode type testing equipment is hand-held electrode type testing equipment, to be easy to
Carry, to realize in outdoor quick detection, reference electrode 25, working electrode 26 and the detection signal acquired in electrode 27 is led to
Cross golden finger 210 and testing equipment is sent to, testing equipment is processed to the detection signal for obtaining, and obtained inspection will be processed
Survey result to show, intuitively check in order to people；210 lower end of the golden finger coordinates institute's shape through upper lid 3 and lower cover 1
Become shell, and the external world is extended to, be arranged such, conveniently golden finger 210 is inserted in the FPDP of testing equipment；Described have
Machine phosphorus insecticide rapid detection card, also includes the end cap 4 for protecting golden finger 210, and end cap 4 is set in lid 3 and lower cover 1 coordinates
The lower end of formed shell, and in golden finger 210 is located at by end cap 4, when the organophosphorus pesticide rapid detection card is not used,
By end cap 4, golden finger 210 is protected, it is to avoid golden finger 210 is broken, use in the organophosphorus pesticide rapid detection card
During, end cap 4 is pulled up, so as to expose golden finger 210, to be inserted in the FPDP of testing equipment；
Be provided with blocked hole 11 on the lower cover 1, on upper lid 3, be provided with the clip-connecting rod 31 corresponding with blocked hole 11, upper lid 3 with
Fastened with the cooperation of blocked hole 11 by clip-connecting rod 31 between lower cover 1 and be connected, the quantity of blocked hole 11 and position are without restriction, this
In embodiment, it is preferred that the blocked hole 11 has four, it is separately positioned at the corner of lower cover 1；Arrange in the lower cover 1
The positioning right angle muscle 12 having for fixed test paper 2, detects that paper 2 is embedded in the locating slot constituted by positioning right angle muscle 12, fixed
Position right angle muscle 12 is made up of upper positioning right angle muscle and lower positioning right angle muscle, and upper positioning right angle muscle has two, and is separately positioned on
Detection 2 upper end both sides of paper, lower positioning right angle muscle has two, is separately positioned on detection 2 lower end both sides of paper, and detects on paper 2 also
It is provided with the locating notch 29 for matching with lower positioning right angle muscle；It is provided with for the limit spacing to detecting paper 2 in the upper lid 3
Position muscle 33, limit rib 33 has two, and is separately positioned on detection 2 middle part both sides of paper.
The manufacture method of the organophosphorus pesticide rapid detection card, step is as follows：
1）Lower cover 1, upper lid 3 and end cap 4 are made by being molded, standby；
2）By way of wax spray is printed, on the filter paper base material of detection paper 2, print hydrophobic layer 21, and the region in non-wax spray
Sample application zone 22, sample presentation passage 23 and detection zone 28 is formed, obtains filter paper after printing；
3）Filter paper after printing is put in baking oven, at 130-135 DEG C, 8-10min is processed, first drying filter paper is obtained, by drying
Dry-cure, makes the wax for being printed upon filter paper substrate surface infiltrate in filter paper base material；
4）Using screen printing technique in the surface printing reference electrode 25 of first drying filter paper, working electrode 26 and to electrode
27, first drying filter paper lower end fix golden finger 210, and make reference electrode 25, working electrode 26 and to electrode 27 all with gold
Finger 210 is connected, and obtains detection paper semi-finished product；
5）Organophosphor hydrolytic enzyme is coated in the reaction enzymes region bar 24 of detection paper semi-finished product, after drying naturally, with detection paper
Organophosphor hydrolytic enzyme layer is formed in the reaction enzymes region bar 24 of semi-finished product, obtain detection paper product；
6）Detection paper product is embedded in lower cover 1, and upper lid 3 is fastened on lower cover 1；
7）End cap 4 is enclosed within the overall bottom constituted by lower cover 1 and 3 fastening of upper lid, golden finger 210 is located at interior.
When the present invention is used, sample drops to sample application zone 22 by adding mouth 32, and the sample in sample application zone 22 passes through sample presentation
Passage 23 is gradually infiltrated in detection zone 28, sample during sample presentation passage 23 is flowed through, can through reaction enzymes region bar 24,
It is fully contacted with organophosphor hydrolytic enzyme layer so as to sample, and mixes with the organophosphor hydrolytic enzyme for carrying, when detection zone 28 is reached,
Sample is mixed with organophosphor hydrolytic enzyme fully, and specific reaction occurs, to be conducive to detection.Now, end cap 4 is pulled out
Under, so as to expose golden finger 210, to be inserted in the FPDP of testing equipment, reference electrode 25, working electrode 26 and right
Detection signal acquired in electrode 27 is sent to testing equipment by golden finger 210, and testing equipment enters to the detection signal for obtaining
Row is processed, and is shown obtained testing result is processed, and is intuitively checked in order to people.
Organophosphorus pesticide rapid detection card of the present invention is easy to use, simple to operate, coordinates hand-held testing equipment, Neng Gouzhi
That seen detects organophosphorus pesticide content in detected sample, and detection speed is fast, can realize the outdoor of organophosphorus pesticide content
Quick detection；Organophosphorus pesticide rapid detection card of the present invention is rational in infrastructure, compared with common papery detection card, is hardly damaged,
It is easy to storage, and extraneous interference can be resisted to a certain extent, advantageously ensures that the accuracy of testing result.
Above the better embodiment of the present invention is explained in detail, but the present invention is not limited to above-mentioned embodiment party
Formula, in the ken that one skilled in the relevant art possesses, can also be on the premise of without departing from present inventive concept
Various changes can be made.
Claims (10)
1. a kind of organophosphorus pesticide rapid detection card, it is characterised in that including lower cover（1）, detection paper（2）With upper lid（3）；Described
Upper lid（3）It is fastened on lower cover（1）On, and upper lid（3）With lower cover（1）Shell is cooperatively formed, detects paper（2）It is fixed on inside the shell；
The detection paper（2）Surface Selective coating has hydrophobic layer（21）, and detect paper（2）The uncoated hydrophobic layer in surface（21）Region
Form sample application zone（22）, sample presentation passage（23）And detection zone（28）, sample application zone（22）And detection zone（28）It is disposed on detection
Paper（2）On, and sample application zone（22）And detection zone（28）Between pass through sample presentation passage（23）It is connected, upper lid（3）On be provided with
Sample application zone（22）The corresponding adding mouth in position（32）；The sample presentation passage（23）Reaction enzymes region bar is inside additionally provided with（24）,
Reaction enzymes region bar（24）Organophosphor hydrolytic enzyme layer is inside coated with；The detection paper（2）On be additionally provided with reference electrode（25）, work
Make electrode（26）With to electrode（27）, reference electrode（25）, working electrode（26）With to electrode（27）Test side be arranged at
Detection zone（28）Interior；The detection paper（2）Lower end is additionally provided with golden finger（210）, reference electrode（25）, working electrode（26）With
To electrode（27）Outfan be respectively connecting to golden finger（210）, and golden finger（210）Relative with the FPDP of testing equipment
Should.
2. organophosphorus pesticide rapid detection card according to claim 1, it is characterised in that reaction enzymes region bar（24）
Three are had, and along sample presentation passage（23）Length direction uniform intervals distribution.
3. organophosphorus pesticide rapid detection card according to claim 2, it is characterised in that the reference electrode（25）With right
Electrode（27）It is separately positioned on working electrode（26）Both sides, and reference electrode（25）With to electrode（27）Top all around work
Make electrode（26）Top around formed arc-shaped.
4. organophosphorus pesticide rapid detection card according to claim 3, it is characterised in that the testing equipment be
Testing equipment.
5. the organophosphorus pesticide rapid detection card according to claim 1 or 2 or 3 or 4, it is characterised in that the golden finger
（210）Lower end passes through upper lid（3）And lower cover（1）Coordinate formed shell, and extend to the external world.
6. organophosphorus pesticide rapid detection card according to claim 5, it is characterised in that also include for protecting golden finger
（210）End cap（4）, end cap（4）It is set in lid（3）And lower cover（1）Coordinate the lower end of formed shell, and end cap（4）Will
Golden finger（210）In being located at.
7. organophosphorus pesticide rapid detection card according to claim 6, it is characterised in that the lower cover（1）On be provided with
Blocked hole（11）, upper lid（3）On be provided with and blocked hole（11）Corresponding clip-connecting rod（31）, upper lid（3）With lower cover（1）Between
By clip-connecting rod（31）With blocked hole（11）Cooperation fasten be connected.
8. organophosphorus pesticide rapid detection card according to claim 7, it is characterised in that the blocked hole（11）Have four
Individual, it is separately positioned on lower cover（1）Corner at.
9. organophosphorus pesticide rapid detection card according to claim 8, it is characterised in that the lower cover（1）Inside it is provided with
For fixed test paper（2）Positioning right angle muscle（12）, detect paper（2）It is embedded in positioning right angle muscle（12）The locating slot for being constituted
Interior, positioning right angle muscle（12）It is made up of upper positioning right angle muscle and lower positioning right angle muscle, upper positioning right angle muscle has two, and respectively
It is arranged on detection paper（2）Upper end both sides, lower positioning right angle muscle has two, is separately positioned on detection paper（2）Lower end both sides, and examine
Survey paper（2）On be additionally provided with the locating notch for matching with lower positioning right angle muscle（29）；The upper lid（3）Inside it is provided with for right
Detection paper（2）Spacing limit rib（33）, limit rib（33）Two are had, and is separately positioned on detection paper（2）Middle part both sides.
10. a kind of manufacture method of organophosphorus pesticide rapid detection card as claimed in claim 9, it is characterised in that step is such as
Under：
1）Lower cover is made by being molded（1）, upper lid（3）And end cap（4）, standby；
2）By way of wax spray is printed, in detection paper（2）Filter paper base material on print hydrophobic layer（21）, and in non-wax spray
Region forms sample application zone（22）, sample presentation passage（23）And detection zone（28）, obtain filter paper after printing；
3）Filter paper after printing is put in baking oven, at 130-135 DEG C, 8-10min is processed, obtain first drying filter paper；
4）Using screen printing technique first drying filter paper surface printing reference electrode（25）, working electrode（26）With to electricity
Pole（27）, golden finger is fixed in first drying filter paper lower end（210）, and make reference electrode（25）, working electrode（26）With to electricity
Pole（27）All and golden finger（210）It is connected, obtains detection paper semi-finished product；
5）Reaction enzymes region bar in detection paper semi-finished product（24）Interior coating organophosphor hydrolytic enzyme, after drying naturally, with detection
The reaction enzymes region bar of paper semi-finished product（24）Interior formation organophosphor hydrolytic enzyme layer, obtains detection paper product；
6）Detection paper product is embedded in lower cover（1）Interior, and by upper lid（3）It is fastened on lower cover（1）On；
7）By end cap（4）It is enclosed within lower cover（1）With upper lid（3）The overall bottom for being constituted is fastened, by golden finger（210）It is located at
Interior.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610926837.8A CN106442660A (en) | 2016-10-31 | 2016-10-31 | Quick detection card for organic phosphorus pesticide and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610926837.8A CN106442660A (en) | 2016-10-31 | 2016-10-31 | Quick detection card for organic phosphorus pesticide and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106442660A true CN106442660A (en) | 2017-02-22 |
Family
ID=58177192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610926837.8A Pending CN106442660A (en) | 2016-10-31 | 2016-10-31 | Quick detection card for organic phosphorus pesticide and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106442660A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109358043A (en) * | 2018-11-01 | 2019-02-19 | 中国农业科学院茶叶研究所 | A method of sample fast detecting pesticide residue is extracted with organic solvent |
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| CN206274316U (en) * | 2016-10-31 | 2017-06-23 | 百奥森（江苏）食品安全科技有限公司 | A kind of organophosphorus pesticide rapid detection card |
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