CN109813900A - A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chloramphenicol - Google Patents
A kind of time-resolved fluoroimmunoassay chromatography quantitative testing test paper item detecting chloramphenicol Download PDFInfo
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- CN109813900A CN109813900A CN201711162962.7A CN201711162962A CN109813900A CN 109813900 A CN109813900 A CN 109813900A CN 201711162962 A CN201711162962 A CN 201711162962A CN 109813900 A CN109813900 A CN 109813900A
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Abstract
The present invention relates to chloramphenicol rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper preparation method and applications.The test strips are made of Fusion5 film, nitrocellulose filter and blotting paper three parts, by competition law principle, using time-resolved fluorescence microballoon as immune marker, carry out quickly and accurately quantitative detection to determined antigen.
Description
Technical field
The invention belongs to field of detection of food safety, and in particular to the detection method of the Harmful Residue in food, especially
Time-resolved fluoroimmunoassay chromatograph test strip of chloramphenicol quantitative detection and its preparation method and application.
Background technique
Chloramphenicol is a kind of broad-spectrum antibiotic, is usually used in the treatment of the various infectious diseases of animal, for a variety of pathogens have compared with
Strong inhibiting effect.There are serious side effects for chloramphenicol, can inhibit human bone marrow's hematopoiesis function, cause the aregeneratory of the mankind
Property the diseases such as anaemia, therefore the residual chloromycetin in animal foodstuff constitutes grave danger to the health of the mankind.
The current measuring method in relation to chloramphenicol in animal derived food and its metabolin is mainly Physico-chemical tests method, is such as used
Chemical analysis measures the chloramphenicol residue in shrimp, and detection is limited to 0.0125ug/kg, uses high effective liquid chromatography for measuring
Chloramphenicol residue in the flesh of fish, detection are limited to 0.0125ug/kg.But these method instrument and equipments are expensive, complicated for operation, sensitive
Spend detection that is low, and not being suitable for batch samples, be unable to satisfy domestic food safety detection market there is an urgent need to.
Current immunochromatography (lateral flow immunoassay, LFIA) Rapid detection test strip is mostly with colloid
Gold, color latex microballoon or fluorescein are as marker.Based on the quick testing product of colloidal gold-labeled method exploitation, exist
The problems such as qualitative or sxemiquantitative, differences between batches are larger；Although color latex microballoon difference between batch improves to some extent, sensitivity is still
It is lower, it also can only qualitative or sxemiquantitative；Immunochromatography sensitivity based on fluorescein labelling technique is greatly improved, also can be into
Row quantitative detection, but due to containing higher autofluorescent background signal in sample, and stock displacement is smaller, can generate to detection
Large effect.
Time-resolved fluorescence (Time-resolved Fluorescence, TRF) is a kind of heterotope fluorescent marker,
Compared with common fluorescent, have stock displacement big, the features such as fluorescence lifetime is long, can effectively avoid the background in sample glimmering
The influence of the stray lights such as light and exciting light, therefore there is higher sensitivity and anti-interference ability compared to common fluorescent.
The present invention substitutes traditional sample pad, blood filter membrane, bonding pad using Fusion5, by original four layers even five layers
Membranous system simplifies, and develops the fast quantification immunity chromatography detection test paper for only needing trilamellar membrane structure, not only obtains production technology
Simplification has been arrived, and has effectively reduced batch internal difference and the difference between batch of product, has improved detection sensitivity, great practical value.
Summary of the invention
The purpose of the invention is to provide the time-resolved fluoroimmunoassay layer of novel energy rapid quantitative detection chloramphenicol
Analyse test strip and its preparation method and application.
One aspect of the present invention discloses chloramphenicol time-resolved fluoroimmunoassay chromatography quantitative testing test paper item, the test strips packet
Include Fusion5 film, nitrocellulose filter and blotting paper three parts.Nitrocellulose filter is located at centre, Fusion5 film and water suction
Paper is overlapped in nitrocellulose filter left and right ends respectively.Wherein, Fusion5 film is equipped with sample application zone and microballoon area, in microballoon area
It is loaded with time-resolved fluorescence microballoon；Nitrocellulose filter is equipped with detection line (T line) and nature controlling line (C line), and T line connects coating chlorine
Mycin antigen, C line are coated with anti-rabbit antibody.
It is an advantage of the invention that the content of chloramphenicol in tissue can be quickly detected.
The chloramphenicol time-resolved fluorescence microballoon, including detection microballoon and Quality Control microballoon, detection microballoon are pan coating
There is the fluorescent microsphere of chloramphenicol monoclonal antibody, Quality Control microballoon is the fluorescent microsphere that pan coating has rabbit-anti labelled protein.
Bright-coloured series elements compound is filled in the fluorescent microsphere；Preferably, which is europium chelating object；
Optimal, which can be Eu(TTA)3/ TOPO or Eu(TTA)3/Phen 。
The albumen can be cow's serum Y-globulin (BGG) or balf serum albumin (BSA).
The time-resolved fluorescence microspherulite diameter range is 100-1000 nm.
On the nitrocellulose filter, coated antigen is chloramphenicol antigen, coated anti-rabbit antibody on C line on T line.
The chloramphenicol time-resolved fluoroimmunoassay quantitative testing test paper bottom is equipped with plastic bottom board.
Second aspect of the present invention discloses the preparation side of chloramphenicol time-resolved fluoroimmunoassay chromatography quantitative testing test paper item
Method, comprising the following steps:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation
Aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of chloramphenicol；
(3) blank kilocalorie is pasted
Using overlapped mode, first stickup nitrocellulose filter on the plastic bottom board with gum, then in cellulose nitrate
Blotting paper and Fusion5 film are pasted respectively in plain film left and right ends；
(4) sprays film
By the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and chloramphenicol monoclonal antibody using release buffer mixed diluting to one
Determine concentration, is sprayed onto the microballoon area of Fusion5 film；After chloramphenicol antigen and the dilution of anti-rabbit antibody, it is sprayed onto nitrocellulose filter respectively
T line and C line position；
(5) is dry and cuts.
By the above-mentioned kilocalorie drying for having sprayed reagent, slitting.
Step (4) in the release buffer used contain 10-15% sucrose, 3-10% trehaloses, 0. 5-1%N, 0-
Double trimethylsilyl acetamides (BSA), 0.1 one 0.5% gentamicins.
Step detects final concentration of 0.5-3 mg/mL of microballoon, Quality Control microballoon final concentration 0.05-0.2 after (4) diluting
mg/mL；0.5-3 mg/mL of T line antigen final concentration, 0.5-23mg/mL of C line antigen final concentration；C, T line spray film liquid amount
0.5-1.5 μ 1/cm, it is 2-10 μ l/cm that microballoon, which sprays film amount,.
Step (5) described in drying can in constant temperature oven or drying chamber, 37 DEG C dry 12-16 hours.
Third aspect present invention discloses the application of chloramphenicol time-resolved fluoroimmunoassay chromatography quantitative testing test paper item.
Test strips of the invention can measure biomolecule by competition law principle, use competition law mode suitable for all
Immunochromatography detection.
Chloramphenicol time-resolved fluoroimmunoassay chromatograph test strip high sensitivity of the invention, withinrun precision is up to 10% left side
The right side, betweenrun precision can detect whole blood, serum, blood plasma, urine specimen, 250 mg/dL hemoglobins, 500 up to 15% simultaneously
Mg/dL triglyceride, 10 mg/dL bilirubin on the detection of this test strips without influence, far more than most of immunochromatographies in the market
Quick diagnosis product.
Detailed description of the invention
Fig. 1 test strips structure schematic diagram of the present invention (1. plastic bottom boards, 2. Fusion5 films, 3. nitrocellulose filters, 4.
Blotting paper, 5. sample application zones, 6. microballoon areas, 7. T line areas, 8. C line areas).
Specific embodiment
1. the preparation of each ingredient of test strips
The preparation for preparing the coated Quality Control microballoon of biotin labeling gamma globulin (BGG) of 1.1 Quality Control microballoons
(1) the preparation of the BGG of biotin labeling
With 0.1 M NaCNBH3BGG(is purchased from Pel-Freez Biological) it is configured to l0 mg/mL solution, it uses
DMSO(dimethylformamide) configuration Biotin-X-X-NHS(N- HOSu NHS modified biological element, manufacturer: SIGMA,
Production number: B3295) solution is to 16.2 mg/mL, according to the amount general of 1 mg BBG albumen addition, 5.4 μ, 1 Biotin-X-X-NHS
Biotin-X-X-NHS liquid is added in BGG solution, is uniformly mixed and is stood overnight at 4 DEG C.It is removed using dialysis free
Unreacted biotin, dialyzate are biotinylated protein elution buffer (0.1 M Tris, 0.3 M Nacl, 0.005 M
EDTA-Na-2H20, pH8.0).Dialysis finishes, and BCA method measures protein concentration.
(2) aldehyde group modified fluorescent microsphere is coated with using above-mentioned labelled protein
The above-mentioned (1) middle dialysis of Tween-20 solution, 2 mg that 6 μ l 20% are added in the fluorescent microsphere aldehyde group modified to 10mL obtains
Biotinylated protein and 16 μ l NaCNBH3, (25 mg/mL, the MES buffer of 0.05 M pH6.0, now match
It is current), adding 0.1 M pH6.0 MES to total volume is 400 μ l, and 37 DEG C are protected from light 48 h of incubation.The Gly solution of 40 μ l is added
(75 mg/mL, the MES buffer of 0.05 M pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l N, 0- bis- three
First silicon substrate acetamide (200 mg/mL, the MES buffer of 0.05 M pH6.0) solution.37 DEG C are protected from light 16 h of revolving reaction.
4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, is washed again twice with the MES buffer of 1 mL pH6.0.With 1 mL, 0.05 M
HEPES buffer solution (the 0.05 M HEPES, 0.3 M Nacl, 0.025 M EDTA-Na-2H of pH8.020,1.6% N, 0-is bis-
Trimethylsilyl acetamide (BSA), 0.1% Dextran, 0.1%Tween-20,0.3745% Triton X- 405,0.0l% celebrating are big
Mycin, 0.05 % Proclin) suspend (its final concentration of l0 mg/mL).
(3) Quality Control microballoon working solution is prepared
According to need of work, (2) respective concentration will be diluted to by middle suspension using the HEPES buffer solution of 0.05M pH 8.0, packing is protected
It deposits.
The preparation of 1.2 detection microballoons
Tween-20 solution, the 2 mg chloramphenicol monoclonal antibodies of 6 μ l 20% are added in the fluorescent microsphere aldehyde group modified to 10 mg
And 16 μ l NaCNBH3(25 mg/mL, the MES buffer of 0.05 M pH6.0 are ready-to-use), adds 0.1 M
PH6.0 MES is 400 μ l to total volume, and 37 DEG C are protected from light 48 h of incubation.Gly solution (75 mg/mL, 0.05 M of 40 μ l are added
The MES buffer of pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l BSA(200 mg/mL, 0.05 M pH6.0
MES buffer) solution.37 DEG C are protected from light 16 h of revolving reaction.4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, with 1
The MES buffer of mL pH6.0 is washed twice again.With the HEPES buffer solution of 1 mL, 0.05 M pH8.0 (0.05 M HEPES, 0.3
M NaC1,0.25 M EDTA-Na-2H20,1.6% BSA, 0.1% Dextran, 0.1% Tween-20,0.3745% Triton
X-405,0.01% gentamicin, 0.05% Proclin) suspend (its final concentration of 10 mg/mL).
The configuration of 1.3 microspheres solutions
With release buffer (contain 20% sucrose, 5% trehalose, 0.5% BSA, 0.2% gentamicin) by Quality Control microballoon and detection
Microballoon is configured to time-resolved fluorescence microballoon mixed liquor, and final concentration of 0.2 mg/mL of Quality Control microballoon detects the final concentration of l of microballoon
mg/mL。
The preparation of 1.4 detection lines (T line) solution
Chloramphenicol antigenic compound is diluted to 0.5 mg/mL with 0.01 M PB solution.
The preparation of 1.5 nature controlling lines (C line) solution
Streptavidin is diluted to 0.5 mg/mL with 0.01 M PB solution.
2. the preparation of test strips
2.1 blank kilocalories are pasted
According to the film combination of attached drawing 1, nitrocellulose filter 3 is located at centre, and Fusion5 film 2 is overlapped in respectively with blotting paper 4
Nitrocellulose filter left and right ends are pasted on the plastic bottom board with gum.
2.2 spray films
T is sprayed in the T line 7 in Fig. 1 respectively, 8 position of C line, and C line solution, C, it is 0.8 μ l/cm that T line, which sprays film liquid amount, 6 in Fig. 1
It sets and sprays microspheres solution, it is 4 μ l/cm that microspheres solution, which sprays film amount,.
2.3 drying
By step (2) in sprayed the chloramphenicol of reagent and be stuck in constant temperature oven 37 DEG C greatly and dry 16 hours.
2.4 slitting
The chloramphenicol kilocalorie of drying is cut into the paper slip of 4 mm width to get chloramphenicol test strips are arrived.
3. detecting instrument principle and application
3.1 measuring instrument principles: condenser lens is located at exciting light into 90 degree of directions, generates the sample in sample detection pond
Fluorescence signal line focus lens focus, then photomultiplier tube is reached after grating beam splitting, photomultiplier tube converts light signals into
Electric signal is finally sent to A/D converter and is handled.The laser light source uses diode pumped solid state laser, output
Power is 20 mW, and launch wavelength is 375 ± 5 nm.Fiber power of the exciting light after conducting fiber coupling is 5 mW or more.
Ultraviolet quartz glass is selected in sample detection pond.Grating is set as that the light for obtaining that wavelength is 440 ± 5nm can be divided.
3.1 detector applications: the extract after pretreatment of the edible animal tissue containing chloramphenicol is packed into sample inspection
It surveys in pond, laser light source issues the laser that wavelength is 375 ± 5nm, conducts after conducting fiber coupling to sample detection pond, and wear
Sample to be tested is crossed, the sample in sample detection pond generates fluorescence after absorbing exciting light, and the fluorescence signal line focus lens of generation are poly-
Coke, then through grating beam splitting, the light that wavelength is 440 ± 5 nm is obtained, photomultiplier tube is reached, photomultiplier tube turns optical signal
Become electric signal, while input a/d converter after electric signal is amplified, finally export and give reading plotter processing, passes through reading dress
Result can directly be read by setting.
4. the quantitative detection of test strips
4.1 draw standard curve
Be added in the chloramphenicol test strips sample application zone prepared various concentration chloramphenicol antigen standard (take five it is different
Concentration, respectively 0,10,30,90,270ng/mL, each concentration set 5 repetitions), sample-loading buffer is added dropwise, and (PBS contains 1.6%
BSA, 0.1% Tween-20, preservative), after film layer is analysed 10 minutes, instrument reading C, T line signal,
Experimental result and analysis are shown in Table 1:
1 chloramphenicol standard items testing result of table
Standard curve is drawn with antigen standard concentration and the signal averaging of measurement, using four parameter fitting modes, standard is bent
Line number evidence is shown in Table 2.
2 chloramphenicol quantitative measurement standard curve data of table
| SPE standard items (ng/mL) | 0 | 10 | 30 | 90 | 270 |
| Reading | 18.918 | 12.48 | 8.536 | 3.706 | 1.44 |
4.2 sample detection
Sample to be tested is sequentially added in the sample application zone of chloramphenicol test strips, sample-loading buffer is added dropwise, after film layer is analysed 10 minutes, instrument
Device reads C, T line signal.According to step (1) in standard curve calculate sample to be tested in chloramphenicol antigen concentration.
5. test strips performance test
10 times crowdes of internal difference CV of 2 concentration samples are measured, experimental result is shown in Table 3, the experimental results showed that, batch interior precision of test strips
Degree is respectively less than 11%.
3 test strips of table batch internal difference
0-500 ng/mL can reach using chloramphenicol quantitative testing test paper detection range prepared by the method, sensitivity exists
For 10 ng/mL hereinafter, withinrun precision is up to 11% or so, betweenrun precision can detect tissue, whole blood, blood up to 15% simultaneously
Clearly, plasma sample, 250 mg/dL hemoglobins, 500 mg/dL triglycerides, 10 mg/dL bilirubin on this detection without influence,
Far more than most of immunochromatography quick diagnosis products in the market.
Claims (10)
1. chloramphenicol rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper, it is characterised in that: including Fusion5 film,
Nitrocellulose filter and blotting paper three parts, nitrocellulose filter are located at centre, and Fusion5 film is overlapped in respectively with blotting paper
There are sample application zone and microballoon area in nitrocellulose filter left and right ends on Fusion5 film, and microballoon area is loaded with chloramphenicol monoclonal antibody
Time-resolved fluorescence microballoon；There are detection line and nature controlling line on nitrocellulose filter, is coated with chloramphenicol antigen, nature controlling line in detection line
Upper coating anti-rabbit antibody.
2. test strips as described in claim 1, it is characterised in that: the time-resolved fluorescence microballoon includes detection microballoon and matter
Microballoon is controlled, detection microballoon is the fluorescent microsphere that pan coating has chloramphenicol monoclonal antibody, and Quality Control microballoon is that pan coating has rabbit-anti label
The fluorescent microsphere of albumen.
3. test strips as claimed in claim 1 or 2, it is characterised in that: the particle size range of the time-resolved fluorescence microballoon is
100 - 1000 nm 。
4. test strips as described in claim 1, it is characterised in that: being coated with chloramphenicol detection in the detection line with antigen is chlorine
The conjugates that mycin and carrier mass are formed；Two anti-rabbit antibody are coated in detection line.
5. the preparation method of test strips as described in claim 1, preparation step are as follows:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation: aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of chloramphenicol；
(3) blank kilocalorie is pasted: nitrocellulose filter being pasted among plastic bottom board, Fusion5 film is taken respectively with blotting paper
It is connected to nitrocellulose filter left and right ends；
(4) sprays film: the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and chloramphenicol monoclonal antibody is mixed using release buffer
It is diluted to a certain concentration, is sprayed onto the microballoon area of Fusion5 film；After chloramphenicol antigen and the dilution of anti-rabbit antibody, it is sprayed onto nitric acid respectively
The T line and C line position of cellulose membrane；
(5) dry and slitting.
6. preparation method as claimed in claim 5, it is characterised in that: (4) middle release buffer group becomes 10-15% sugarcanes to step
Sugar, 3-10% trehaloses, 0. 5-1% N, the bis- trimethylsilyl acetamides (BSA) of O-, 0.1-0.5% gentamicin.
7. preparation method as claimed in claim 5, it is characterised in that: step detects microballoon final concentration of 0.5-after (4) diluting
3mg/mL, 0.05-0.3 mg/mL of Quality Control microballoon final concentration；0.5-3mg/mL of T line antigen final concentration, C line antigen final concentration
0. 5 - 3 mg/mL；It is 0.5-1.5 μ 1/cm that C, T line, which spray film liquid amount, and it is 2-10 μ l/cm that microballoon, which sprays film amount,.
8. preparation method as claimed in claim 5, it is characterised in that: (5) middle drying condition is 37 DEG C of drying 12-16 to step
Hour.
9. the chloramphenicol rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper as described in claim 5 claim,
It is characterized by: test strips bottom is equipped with plastic bottom board.
10. the application of test strips as described in claim 1, it is characterised in that: the test strips are used for using competition law mode
The quantitative detection of immunochromatography.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110658342A (en) * | 2019-10-12 | 2020-01-07 | 石家庄市畜产品质量监测中心 | Time-resolved fluorescence immunochromatographic assay quantitative detection method for chloramphenicol in aquatic products |
| CN110702896A (en) * | 2019-10-12 | 2020-01-17 | 青海省动物疫病预防控制中心 | Time-resolved fluorescence immunochromatographic assay quantitative detection method for quinolone drugs in yak meat |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101435823A (en) * | 2007-11-12 | 2009-05-20 | 无锡中德伯尔生物技术有限公司 | Fluorescent micro-ball immune chromatography test paper strip for detecting residual animal medicine and preparing method thereof |
| CN102353774A (en) * | 2011-06-13 | 2012-02-15 | 清华大学深圳研究生院 | Immunochromatographic test paper for detecting chloramphenicol and its preparation method |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104169710A (en) * | 2013-03-19 | 2014-11-26 | 香港锦程发展有限公司 | Method and apparatus for time-resolved fluorescence immunoassay testing |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN105319369A (en) * | 2014-07-24 | 2016-02-10 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit used for detecting chloramphenicol, and detection method thereof |
| CN105424923A (en) * | 2015-09-10 | 2016-03-23 | 南京微测生物科技有限公司 | Color-fluorescence dual-function immunochromatography test strip and preparation method thereof |
| CN106405073A (en) * | 2015-08-03 | 2017-02-15 | 南京亿特生物科技有限公司 | Colloidal-gold detection card for cyhalothrin |
-
2017
- 2017-11-21 CN CN201711162962.7A patent/CN109813900A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101435823A (en) * | 2007-11-12 | 2009-05-20 | 无锡中德伯尔生物技术有限公司 | Fluorescent micro-ball immune chromatography test paper strip for detecting residual animal medicine and preparing method thereof |
| CN102353774A (en) * | 2011-06-13 | 2012-02-15 | 清华大学深圳研究生院 | Immunochromatographic test paper for detecting chloramphenicol and its preparation method |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104169710A (en) * | 2013-03-19 | 2014-11-26 | 香港锦程发展有限公司 | Method and apparatus for time-resolved fluorescence immunoassay testing |
| CN104655835A (en) * | 2013-11-20 | 2015-05-27 | 南京亿特生物科技有限公司 | Immune colloidal gold detect card of chlorpromazine, and preparation method thereof |
| CN105319369A (en) * | 2014-07-24 | 2016-02-10 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit used for detecting chloramphenicol, and detection method thereof |
| CN106405073A (en) * | 2015-08-03 | 2017-02-15 | 南京亿特生物科技有限公司 | Colloidal-gold detection card for cyhalothrin |
| CN105424923A (en) * | 2015-09-10 | 2016-03-23 | 南京微测生物科技有限公司 | Color-fluorescence dual-function immunochromatography test strip and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110658342A (en) * | 2019-10-12 | 2020-01-07 | 石家庄市畜产品质量监测中心 | Time-resolved fluorescence immunochromatographic assay quantitative detection method for chloramphenicol in aquatic products |
| CN110702896A (en) * | 2019-10-12 | 2020-01-17 | 青海省动物疫病预防控制中心 | Time-resolved fluorescence immunochromatographic assay quantitative detection method for quinolone drugs in yak meat |
| CN110658342B (en) * | 2019-10-12 | 2023-06-02 | 石家庄市畜产品质量监测中心 | Method for quantitatively detecting chloramphenicol in aquatic products through time-resolved fluorescence immunochromatography |
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Application publication date: 20190528 |