CN204237788U - A kind of closed culture dish - Google Patents

A kind of closed culture dish Download PDF

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Publication number
CN204237788U
CN204237788U CN201420664257.2U CN201420664257U CN204237788U CN 204237788 U CN204237788 U CN 204237788U CN 201420664257 U CN201420664257 U CN 201420664257U CN 204237788 U CN204237788 U CN 204237788U
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China
Prior art keywords
ware
culture dish
jiao
hole
ware lid
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CN201420664257.2U
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Chinese (zh)
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张静霞
韩涛
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Putian University
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Putian University
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Abstract

The utility model discloses a kind of closed culture dish, described closed culture dish to comprise at the bottom of ware and can with the ware lid closely fastened at the bottom of ware, diapire and ware downside wall is comprised at the bottom of ware, ware lid comprises tegmental wall and ware lid sidewall, be provided with friction sealed device at the contact surface of ware downside wall and ware lid sidewall, limit the resistance to air loss of described contact surface.Described tegmental wall is provided with through hole or aperture, millipore filtration and stopple are placed in through hole, or strainer is placed in aperture by apron, the structure determined accordingly, can air component stable of maintain ware inner chamber as the passage of gaseous interchange, keeps the balance of culture dish inner and outer air pressure, prevent the explosion with ware lid at the bottom of ware, also can filter the air flowed through, stop passing through and diffuse pollution of harmful gaseous solutions such as the thalline of pathogenic microorganism in culture dish or spore, ensure the safety of operator.

Description

A kind of closed culture dish
Technical field
The utility model relates to a kind of experiment equipment, particularly a kind of closed culture dish.
Background technology
Culture dish is one of experiment equipment the most frequently used in biology, and be widely used in the cultivation of microorganism, histiocyticly go down to posterity, culture dish conventional at present generally comprises at the bottom of ware and the lax ware lid be covered at the bottom of ware, and the two inner chamber formed is as culture space.Culture dish is inserted in the incubator of a temperature-adjustable, humidity, to set up the top condition of culture growth.Air in incubator has the oxygen and carbon dioxide content determined, and the lax ware lid supported allows the air of culture dish inner chamber and incubator to form gaseous interchange.But, when opening ware lid or culture dish flow of external air, the turbulent flow of air in culture dish may be caused, and then make the gaseous solution suspended in air in culture dish (comprise vaporific little liquid or solid granule etc., thalline or spore, gemma etc. as microorganism) air release towards periphery, pollute surrounding environment, this is at cultivation pathogenic microorganism or especially dangerous when producing ectotoxic cell, and therefore user also may bear Health hazard.
CN101875902 B discloses a kind of Tissue Culture Dish, to comprise at the bottom of a ware and sealing is placed in ware lid on ware downside wall, and ware lid and the contact surface at the bottom of ware are friction lock locking devices, and the sealed state of formation can by the bore seal of culture dish.This culture dish is also provided with ventilation gap in addition, and under ventilation state, ventilation gap can as the passage of gaseous interchange inside and outside culture dish.Ventilation state and sealed state can be exchanged easily by manual shift, to adapt to the demand of different culture condition.But, the sealed state of this Tissue Culture Dish and ventilation state cannot be changed automatically, and when culture dish is in sealed state, inside and outside culture dish, the exchange of gas is completely blocked, this may affect the normal growth breeding of culture in culture dish, particularly aerobic microorganism.In addition when the culture dish being in sealed state is for cultivating the larger microorganism of some gas production rate, the gas that bacteria metabolism produces will make culture dish internal gas pressure raise gradually, and then cause at the bottom of ware and the explosion of ware lid, and the splashing of culture or substratum in culture dish, thus cause the failure of cultivation and pollute on a large scale.
The culture dish product tackiness agent had in addition by close adhesion removable at the bottom of ware lid and ware, to form the space of culture dish internal independence, sealing.But through storing for a long time, after the cultivation of different temperature and humidity conditions or autoclave sterilization, the characteristic of tackiness agent may change, thus affects its sealing effectiveness.The internal substance of tackiness agent also may pollute substratum, affects the growth of culture in culture dish.The culture dish of adhesive seal also prevents the exchange of gas inside and outside culture dish, also may affect aerobic microbial growth in culture dish or cause at the bottom of ware and the explosion of ware lid, the splashing of culture and substratum in culture dish.
Summary of the invention
For above-mentioned shortcoming, the purpose of this utility model is the closed culture dish providing a kind of structure simple, easy to operate, the air component of maintain ware inner chamber stable, simultaneously, stop the diffusion of the gaseous solution of culture dish inner suspension, prevent at the bottom of ware and the explosion of ware lid.
For closed culture dish, for reaching this object, the technical solution adopted in the utility model is:
A kind of closed culture dish, to comprise at the bottom of ware and can with seal the ware lid fastened at the bottom of ware, it is characterized in that:
Comprise at the bottom of described ware diapire and at least one from diapire around, tilt protrude ware downside wall, the angle of diapire and ware downside wall is first jiao, and described first jiao is acute angle;
Described ware lid comprise tegmental wall and at least one from tegmental wall around, tilt protrude ware lid sidewall, the angle of tegmental wall and ware lid sidewall is second jiao, and described second jiao is obtuse angle;
Described first jiao with described second jiao of supplementary angle each other;
At the contact surface of described ware downside wall and described ware lid sidewall, ware downside wall place has the first friction sealed device, ware lid side-walls has the second friction sealed device;
Described tegmental wall is provided with at least one through hole, the non-through tegmental wall of described through hole, is provided with at least one first hole in the bottom of through hole, the described through tegmental wall of the first hole.
Closed culture dish, is characterized in that: also comprise millipore filtration or stopple, described millipore filtration or stopple removable be placed in described through hole, described stopple is provided with at least one second hole.
Closed culture dish, is characterized in that: also comprise at least one and be positioned at first of described ware downside wall and grasp projection, or at least one is positioned at second of described ware lid sidewall and grasps projection.
Closed culture dish, is characterized in that: also comprise the first projection that at least one is positioned at described diapire, or at least one is positioned at the second projection of described tegmental wall.
Closed culture dish, is characterized in that: at the bottom of described ware or being made up of rigid material at least partially of described ware lid, and described rigid material comprises glass, synthetic resins or corrosion resistant metal etc.; Described stopple at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
A kind of closed culture dish, to comprise at the bottom of ware and can with seal the ware lid fastened at the bottom of ware, it is characterized in that:
Comprise at the bottom of described ware diapire and at least one from diapire around, tilt protrude ware downside wall, the angle of diapire and ware downside wall is first jiao, and described first jiao is acute angle;
Described ware lid comprise tegmental wall and at least one from tegmental wall around, tilt protrude ware lid sidewall, the angle of tegmental wall and ware lid sidewall is second jiao, and described second jiao is obtuse angle;
Described first jiao with described second jiao of supplementary angle each other;
At the contact surface of described ware downside wall and described ware lid sidewall, ware downside wall place has the first friction sealed device, ware lid side-walls has the second friction sealed device;
Described tegmental wall comprises at least one aperture, and described aperture runs through tegmental wall.
Closed culture dish, is characterized in that: also comprise apron or stopple or strainer, described apron or stopple or strainer removable be placed in described aperture, described stopple is provided with at least one second hole.
Closed culture dish, is characterized in that: also comprise at least one and be positioned at first of described ware downside wall and grasp projection, or at least one is positioned at second of described ware lid sidewall and grasps projection.
Closed culture dish, is characterized in that: also comprise the first projection that at least one is positioned at described diapire, or at least one is positioned at the second projection of described tegmental wall.
Closed culture dish, is characterized in that: at the bottom of described ware or being made up of rigid material at least partially of described ware lid, and described rigid material comprises glass, synthetic resins or corrosion resistant metal etc.; Described apron or stopple at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
Compared with the prior art, the beneficial effects of the utility model are:
At the bottom of ware and after the conjunction of ware cover buckle, the first friction sealed device of ware downside wall and second of ware lid sidewall the friction sealed device coordinate, and limit at the bottom of ware and contact the resistance to air loss at gap place with ware lid.
Millipore filtration is placed in through hole, cover with stopple again, the structure determined accordingly has limited ventilative feature (can keep the ventilation property of through hole, the air flowed through can be filtered again): on the one hand, millipore filtration is combined with the first hole of through hole and the second hole of stopple, the passage that closed culture dish inner chamber gas and extraneous air carry out gaseous interchange can be set up, maintain the stable of the air component of closed culture dish inner chamber, therefore this culture dish can not make aerobic microorganism cannot normal growth because of anoxic.In addition, the existence of this passage makes closed culture dish inner and outer air pressure keep balance, effectively can prevent the explosion with ware lid at the bottom of ware, and the splashing of culture or substratum.
On the other hand, the millipore filtration in through hole can filter the air flowed through, and stops passing through of the gaseous solution of culture dish inner suspension.Millipore filtration is combined with friction sealed device, effectively can prevent the thalline of pathogenic microorganism in culture dish or the diffusion of the objectionable impurities such as spore, gemma and pollution, ensures the safety of operator.
In another technical scheme, strainer is placed in aperture closely by apron, also can reaches limited ventilative target.Strainer can keep the ventilation property of aperture, also can filter the air flowed through, and also can realize the filtration identical with millipore filtration and function of safety protection.
Accompanying drawing explanation
In order to clearer explanation the technical solution of the utility model, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the embodiment 1 of closed culture dish;
Fig. 2 is the longitudinal section of embodiment 1;
Fig. 3 be multiple embodiment 1 stacking place time longitudinal section;
Fig. 4 is the close-up sectional view of the through hole of embodiment 1;
Fig. 5 is the planing surface schematic diagram of the stopple of embodiment 1;
Fig. 6 is the longitudinal section of the embodiment 2 of closed culture dish;
Fig. 7 is the strainer of embodiment 2 and the close-up sectional view of aperture;
Fig. 8 is the planing surface schematic diagram of the apron of embodiment 2;
Fig. 9 is the planing surface schematic diagram of the stopple of embodiment 2;
Figure 10 is the longitudinal section of the embodiment 3 of closed culture dish;
Figure 11 is the longitudinal section of the embodiment 4 of closed culture dish;
Figure 12 is the longitudinal section of the embodiment 5 of closed culture dish.
In figure:
Ware lid described in 1-embodiment 1, the tegmental wall described in 2-embodiment 1,
Ware lid sidewall described in 3-embodiment 1, the second friction gear described in 4-embodiment 1,
At the bottom of ware described in 5-embodiment 1, the diapire described in 6-embodiment 1,
Ware downside wall described in 7-embodiment 1, the first friction gear described in 8-embodiment 1,
Through hole described in 9-embodiment 1, second described in 10-embodiment 1 grasps projection,
Described in 11-embodiment 1 first grasps projection, and first described in 12-embodiment 1 is protruding, and second described in 13-embodiment 1 is protruding, the first hole described in 14-embodiment 1,
Stopple described in 15-embodiment 1, the second hole described in 16-embodiment 1,
Millipore filtration described in 17-embodiment 1, second described in ∠ 18-embodiment 1 jiao,
Described in ∠ 19-embodiment 1 first jiao;
Ware lid described in 1 '-embodiment 2, the tegmental wall described in 2 '-embodiment 2,
Ware lid sidewall described in 3 '-embodiment 2, the second friction gear described in 4 '-embodiment 2,
At the bottom of ware described in 5 '-embodiment 2, the diapire described in 6 '-embodiment 2,
Ware downside wall described in 7 '-embodiment 2, the first friction gear described in 8 '-embodiment 2,
Through hole described in 9 '-embodiment 2, second described in 10 '-embodiment 2 grasps projection,
Described in 11 '-embodiment 2 first grasps projection, and first described in 12 '-embodiment 2 is protruding,
Described in 13 '-embodiment 2 second is protruding, the stopple described in 15 '-embodiment 2,
The second hole described in 16 '-embodiment 2, second described in ∠ 18 '-embodiment 2 jiao,
Described in ∠ 19 '-embodiment 2 first jiao, the strainer described in 20 '-embodiment 2,
Apron described in 21 '-embodiment 2;
1 " the ware lid described in-embodiment 3,2 " tegmental wall described in-embodiment 3,
3 " the ware lid sidewall described in-embodiment 3,5 " at the bottom of the ware described in-embodiment 3,
6 " diapire described in-embodiment 3,7 " the ware downside wall described in-embodiment 3,
9 " through hole described in-embodiment 3, ∠ 18 " second described in-embodiment 3 jiao,
∠ 19 " first described in-embodiment 3 jiao;
1 ' " the ware lid described in-embodiment 4,5 ' " at the bottom of the ware described in-embodiment 4,
9 ' " through hole described in-embodiment 4, ∠ 18 ' " second described in-embodiment 4 jiao,
∠ 19 ' " first described in-embodiment 4 jiao;
1 " the ware lid described in "-embodiment 5,5 " " at the bottom of the ware described in-embodiment 5,
9 " through hole described in "-embodiment 5, ∠ 18 " " second described in-embodiment 5 jiao,
∠ 19 " " first described in-embodiment 5 jiao.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage more clear, below in conjunction with accompanying drawing, illustrate 5 preferred embodiments, be described in further detail the utility model, 5 embodiments are followed successively by: embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5.Should be appreciated that described embodiment only in order to explain the utility model, be not used in restriction the utility model.Every simplification of doing based on the technical solution of the utility model or equivalent variations, all belong to protection domain of the present utility model.
In the discussion of different embodiment, identical mark is adopted for corresponding feature, and the differently composed footmark by above putting of other embodiment is indicated, footmark (') corresponding embodiment 2, footmark (") corresponding embodiment 3; footmark (' ") corresponding embodiment 4, footmark (" ") corresponding embodiment 5.The shape of the closed culture dish described in 5 embodiments is selected preferably circular, but those skilled in the art it should be understood that the utility model is also applicable to such as oval, rectangle or other is applicable to the shape of culture dish.
The utility model discloses a kind of closed culture dish, Fig. 1 to Fig. 5 illustrates preferred embodiment 1, described closed culture dish to comprise at the bottom of ware 5 and can with 5 seal the ware lid 1 fastened at the bottom of ware; At the bottom of described ware 5 comprise diapire 6 and at least one from diapire 6 around, the ware downside wall 7 that tilts to protrude, diapire 6 is first jiao of ∠ 19 with the angle of ware downside wall 7, and described first jiao of ∠ 19 is acute angle; Described ware lid 1 comprise tegmental wall 2 and at least one from tegmental wall 2 around, the ware lid sidewall 3 that tilts to protrude, tegmental wall 2 is second jiao of ∠ 18 with the angle of ware lid sidewall 3, and described second jiao of ∠ 18 is obtuse angle.
Described first jiao of ∠ 19 and described second jiao of ∠ 18 supplementary angle each other.Described diapire 6 is of similar shape with described tegmental wall 2, determines the shape of described closed culture dish simultaneously.At the bottom of the ware that ware lid 1 described in above-mentioned 2 features limit is lucky and described, 5 fasten closely.
At the contact surface of described ware downside wall 7 with described ware lid sidewall 3, ware downside wall 7 place has the first friction sealed device 8, ware lid sidewall 3 place has the second friction sealed device 4, and the described first friction sealed device 8 coordinates the resistance to air loss of the contact surface limiting described ware downside wall 7 and described ware lid sidewall 3 with the second friction sealed device 4.
Described tegmental wall 2 is provided with at least one through hole 9, the non-through tegmental wall 2 of described through hole 9, is provided with at least one first hole 14 in the bottom of through hole 9, the described through tegmental wall 2 of the first hole 14.In addition, through hole 9 can be recessed to form by tegmental wall 2.
As improvement, described closed culture dish can also comprise millipore filtration 17, and what described millipore filtration 17 was removable is placed in described through hole 9.
As improvement, described closed culture dish can also comprise the removable stopple 15 be placed in described through hole 9, and described stopple 15 has at least one second hole 16.
Those skilled in the art it should be understood that the shape of through hole 9, millipore filtration 17 or stopple 15 involved in above-described embodiment is identical, preferably select circle, but the utility model is also applicable to such as ellipse, rectangle or other possible shape.The diameter of described millipore filtration 17 is identical with the diameter of through hole 9, and the diameter of stopple 15 is slightly larger than the diameter of through hole 9.Described stopple 15 mode be placed in through hole 9 can select direct insertion or screw thread screwing type, and other possible mode, and the stopple 15 of Fig. 4 display is placed in through hole 9 with direct insertion.
Described millipore filtration 17 is placed in through hole 9, then covers (as shown in Figure 4) with stopple 15, and the structure set up accordingly has limited ventilative feature, can keep the ventilation property of through hole 9, can filter again the air flowed through.In this structure, described has certain elastic stopple 15 for closely fixing millipore filtration 17, also can determine the resistance to air loss of stopple 15 and through hole 9 contact surface.Therefore, air-flow is defined in specific passage, and namely air can only to be combined in the gas channel of foundation with the first hole 14 of through hole 9 and the second hole 16 of stopple 15 at millipore filtration 17 and circulated.
Described gas channel carries out the necessary ways exchanged as gas inside and outside closed culture dish, can air component stable of maintain ware inner chamber, and therefore this culture dish can not make aerobic microorganism cannot normal growth because of anoxic.In addition, the existence of this gas channel makes closed culture dish inner and outer air pressure keep balance, can effectively to prevent at the bottom of ware 5 with the explosion of ware lid 1, and the splashing of culture or substratum.
On the other hand, the millipore filtration 17 in through hole 9 can filter the air flowed through, and stops passing through of the gaseous solution of culture dish inner suspension.Millipore filtration 17 is combined with the friction sealed device 4 of the first friction sealed device 8, second, effectively can stop the thalline of pathogenic microorganism in culture dish or the diffusion of the objectionable impurities such as spore, gemma and pollution, ensures the safety of operator.
When not placing millipore filtration 17 in through hole 9, air directly flows through through hole 9 without the filtration of millipore filtration, larger Air permenbility can be provided, to meet the demand of corresponding culture condition, such as, when being used for cultivating nontoxic cell by closed culture dish for culture dish.
Those of ordinary skill in the art it should be understood that described millipore filtration 17 can select different aperture specifications to adapt to different demands, preferably selects the millipore filtration in 0.22 μm of aperture.
As improvement, described closed culture dish can also comprise at least one and be positioned at first of described ware downside wall 7 and grasp projection 11, or at least one is positioned at second of described ware lid sidewall 3 and grasps projection 10.First grasps cooperation that projection 11 and second grasps projection 10 can make described ware lid 1 and 5 open more easily or fasten at the bottom of ware.
As improvement, described closed culture dish can also comprise the first projection 12 that at least one is positioned at described diapire 6, or at least one is positioned at the second projection 13 of described tegmental wall 2, and described first projection 12 is identical with the shape of described second projection 13.In the embodiment that Fig. 3 shows, the diameter of described first projection 12 is slightly less than described second projection 13, but those skilled in the art it should be understood that the diameter of described first projection 12 in other embodiments also can slightly larger than the size of described second projection 13.The acting body of the described first protruding 12 and second projection 13 is present, when multiple described closed culture dish is arranged to stacked structure (as shown in Figure 3), first protruding 12 of a culture dish and second protruding 13 of adjacent culture dish cooperates, and can limit the movement of a described culture dish relative to described adjacent culture dish.
At the bottom of the ware of described closed culture dish 5 or being made up of rigid material at least partially of ware lid 1, described rigid material comprises glass, synthetic resins or corrosion resistant metal etc., preferably select rigidity, transparent and after high pressure steam sterilization indeformable material, such as glass, polymeric material etc.
The stopple 15 of described closed culture dish at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
For closed culture dish, in another preferred embodiment 2 of the utility model (as shown in Figures 6 to 9), described closed culture dish to comprise at the bottom of ware 5 ' and can with 5 ' seal the ware lid 1 ' fastened at the bottom of ware; At the bottom of described ware 5 ' comprise diapire 6 ' and at least one from diapire 6 ' around, the ware downside wall 7 ' that tilts to protrude, diapire 6 ' is first jiao of ∠ 19 ' with the angle of ware downside wall 7 ', and first jiao of described ∠ 19 ' is acute angle; Described ware lid 1 ' comprise tegmental wall 2 ' and at least one from tegmental wall 2 ' around, the ware lid sidewall 3 ' that tilts to protrude, tegmental wall 2 ' is second jiao of ∠ 18 ' with the angle of ware lid sidewall 3 ', and second jiao of described ∠ 18 ' is obtuse angle.
Described first jiao of ∠ 19 ' and described second jiao of ∠ 18 ' supplementary angle each other.Described diapire 6 ' is of similar shape with described tegmental wall 2 ', determines the shape of described closed culture dish simultaneously.At the bottom of the ware that ware lid 1 ' described in above-mentioned 2 features limit is lucky and described, 5 ' fastens closely.
At the contact surface of described ware downside wall 7 ' with described ware lid sidewall 3 ', ware downside wall 7 ' place has the first friction sealed device 8 ', ware lid sidewall 3 ' place has the second friction sealed device 4 ', and the described first friction sealed device 8 ' coordinates the resistance to air loss of the contact surface limiting described ware downside wall 7 ' and described ware lid sidewall 3 ' with the second friction sealed device 4 '.
Described tegmental wall 2 ' is provided with at least one aperture 9 ', and the described non-through tegmental wall 2 ' of aperture 9 ', aperture 9 ' can be recessed to form by tegmental wall 2 '.
As improvement, described closed culture dish can also comprise the removable stopple 15 ' be placed in described aperture 9 ', and described stopple 15 ' has at least one second hole 16 '.
As improvement, described closed culture dish can also comprise and removable is placed in apron 21 ' in described aperture 9 ' or strainer 20 ', and described apron 21 ' is for Fixed-Filter 20 '.Described strainer 20 ' can select some more ripe commercially produced product, the Acrodisc strainer of the Millex-GP strainer that such as Merck Millipore company produces and Millex-HV strainer and the production of Caliper Life Science company.Meanwhile, described strainer 20 ' can select different aperture specifications (as 0.2 μm, 0.22 μm or 0.45 μm etc.) to adapt to different demands.
Those skilled in the art it should be understood that the shape of aperture 9 ', apron 21 ' or stopple 15 ' involved in above-described embodiment is identical, preferably select circle, but the utility model is also applicable to such as ellipse, rectangle or other possible shape.The diameter of described apron 21 ' and stopple 15 ' is slightly larger than the diameter of aperture 9 '.Described apron 21 ' and the stopple 15 ' mode be placed in aperture 9 ' can select direct insertion or screw thread screwing type, and other possible mode, and the apron 21 ' of Fig. 7 display is placed in aperture 9 ' with direct insertion.
When described apron 21 ' is placed in aperture 9 ', and when apron 21 ' upper closely placement strainer 20 ', there is certain elastic apron 21 ', the resistance to air loss of apron 21 ' and aperture 9 ' contact surface can be determined, and the resistance to air loss of apron 21 ' and strainer 20 ' contact surface, limiting air can only be circulated by strainer 20 '.Strainer 20 ' now also has limited ventilative feature, can keep aperture 9 ' ventilative while, filter the air flowed through, also can realize the filtration identical with millipore filtration 17 and function of safety protection.
Described strainer 20 ' carries out the necessary ways exchanged as gas inside and outside closed culture dish, can air component stable of maintain ware inner chamber, and therefore this culture dish can not make aerobic microorganism cannot normal growth because of anoxic.In addition, the existence of described strainer 20 ' makes closed culture dish inner and outer air pressure keep balance, can effectively to prevent at the bottom of ware 5 ' with the explosion of ware lid 1 ', and the splashing of culture or substratum.
On the other hand, the strainer 20 ' in aperture 9 ' can filter the air flowed through, and stops passing through of the gaseous solution of culture dish inner suspension.Strainer 20 ' is combined with the first friction sealed device 8 ', the second friction sealed device 4 ', effectively can stop the thalline of pathogenic microorganism in culture dish or the diffusion of the objectionable impurities such as spore, gemma and pollution, ensures the safety of operator.
Described stopple 15 ' is placed in aperture 9 ', oarse-grained pollutent can be prevented through aperture 9 ', and the second hole 16 ' being positioned at stopple 15 ' can provide larger Air permenbility for culture dish, to meet the demand of corresponding culture condition, such as, when closed culture dish being used for cultivating nontoxic cell.
As improvement, described closed culture dish can also comprise at least one and be positioned at first of described ware downside wall 7 ' and grasp projection 11 ', or at least one is positioned at second of described ware lid sidewall 3 ' and grasps projection 10 '.First grasps cooperation that projection 11 ' and second grasps projection 10 ' can make described ware lid 1 ' and 5 ' open more easily or fasten at the bottom of ware.
As improvement, described closed culture dish can also comprise the first projection 12 ' that at least one is positioned at described diapire 6 ', or at least one is positioned at the second projection 13 ' of described tegmental wall 2 '.Described first projection 12 ' is identical with the shape of described second projection 13 ', its acting body is present, when multiple described closed culture dish is arranged to stacked structure, first projection 12 ' of a culture dish cooperates with the second projection 13 ' of adjacent culture dish, can limit the movement of a described culture dish relative to described adjacent culture dish.
At the bottom of the ware of described closed culture dish 5 ' or being made up of rigid material at least partially of ware lid 1 ', described rigid material comprises glass, synthetic resins or corrosion resistant metal etc., preferably select rigidity, transparent and after high pressure steam sterilization indeformable material, such as glass, polymeric material etc.
The apron 21 ' of described closed culture dish or stopple 15 ' at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
In another preferred embodiment 3 of closed culture dish (as shown in Figure 10), described diapire 6 ' ' with described ware downside wall 7 ' ', forms a right angle, forms an acute angle (being namely first jiao of ∠ 19 ' ') in outside inside culture dish; Described tegmental wall 2 ' ' with described ware lid sidewall 3 ' ', forms a right angle, forms an obtuse angle (being namely second jiao of ∠ 18 ' ') in inner side outside culture dish.First jiao of described ∠ 19 ' ' and described second jiao of ∠ 18 ' ' supplementary angle each other.Described in embodiment 3, the profile of closed culture dish is smooth, to be beneficial at the bottom of ware 5 ' ' and ware lid 1 ' ' grasps, and can increase stability when multiple described closed culture dish is arranged to stacked structure.The inner chamber that described diapire 6 ' ' with described ware downside wall 7 ' ' limits is cylindrical, can carry out experimental implementation more easily or result is observed.
For closed culture dish, embodiment 4(is as shown in figure 11) and embodiment 5(is as shown in figure 12) be the distortion of embodiment 3.Ware lid 1 ' described in embodiment 4 ' ' structure and feature be equal to the ware lid 1 described in embodiment 1, at the bottom of described ware 5 ' ' ' structure and feature be equal at the bottom of the ware described in embodiment 35 ' '.Structure and the feature of the ware lid 1 ' described in embodiment 5 ' ' ' are equal to the ware lid 1 ' described in embodiment 3 ', at the bottom of described ware 5 ' ' ' ' structure and feature to be equal at the bottom of the ware described in embodiment 15.
The structure of Figure 10, Figure 11 and the through hole shown in Figure 12 (comprise through hole 9 ' ', through hole 9 ' ' ' and through hole 9 ' ' ' ') is similar to the through hole 9 described in embodiment 1 with feature, but, should be appreciated that through hole 9 ' ' or through hole 9 ' ' ' or through hole 9 ' ' ' ' also can be designed as the structure and feature that are similar to the aperture 9 ' described in embodiment 2.
For embodiment 3, embodiment 4 or embodiment 5, except above-mentioned feature, all the other all structures and feature all with embodiment 1 or embodiment 2 similar.Embodiment 3, embodiment 4 or embodiment 5 all can reach the beneficial effect identical with embodiment 1 or embodiment 2.
More than by description of listed embodiment, basic conception of the present utility model and ultimate principle are set forth.Obviously, described embodiment is only a part of embodiment of the present utility model, and indefiniteness is exhaustive.Based on the embodiment in the utility model, other embodiments all that those of ordinary skill in the art obtain under the prerequisite not paying creative work, all belong to protection domain of the present utility model.

Claims (10)

1. a closed culture dish, to comprise at the bottom of ware and can with seal the ware lid fastened at the bottom of ware, it is characterized in that:
Comprise at the bottom of described ware diapire and at least one from diapire around, tilt protrude ware downside wall, the angle of diapire and ware downside wall is first jiao, and described first jiao is acute angle;
Described ware lid comprise tegmental wall and at least one from tegmental wall around, tilt protrude ware lid sidewall, the angle of tegmental wall and ware lid sidewall is second jiao, and described second jiao is obtuse angle;
Described first jiao with described second jiao of supplementary angle each other;
At the contact surface of described ware downside wall and described ware lid sidewall, ware downside wall place has the first friction sealed device, ware lid side-walls has the second friction sealed device;
Described tegmental wall is provided with at least one through hole, the non-through tegmental wall of described through hole, is provided with at least one first hole in the bottom of through hole, the described through tegmental wall of the first hole.
2. closed culture dish according to claim 1, is characterized in that: also comprise millipore filtration or stopple, described millipore filtration or stopple removable be placed in described through hole, described stopple is provided with at least one second hole.
3. closed culture dish according to claim 1, is characterized in that: also comprise at least one and be positioned at first of described ware downside wall and grasp projection, or at least one is positioned at second of described ware lid sidewall and grasps projection.
4. closed culture dish according to claim 1, is characterized in that: also comprise the first projection that at least one is positioned at described diapire, or at least one is positioned at the second projection of described tegmental wall.
5. closed culture dish according to claim 1 and 2, is characterized in that: at the bottom of described ware or being made up of rigid material at least partially of described ware lid, and described rigid material comprises glass, synthetic resins or corrosion resistant metal etc.; Described stopple at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
6. a closed culture dish, to comprise at the bottom of ware and can with seal the ware lid fastened at the bottom of ware, it is characterized in that:
Comprise at the bottom of described ware diapire and at least one from diapire around, tilt protrude ware downside wall, the angle of diapire and ware downside wall is first jiao, and described first jiao is acute angle;
Described ware lid comprise tegmental wall and at least one from tegmental wall around, tilt protrude ware lid sidewall, the angle of tegmental wall and ware lid sidewall is second jiao, and described second jiao is obtuse angle;
Described first jiao with described second jiao of supplementary angle each other;
At the contact surface of described ware downside wall and described ware lid sidewall, ware downside wall place has the first friction sealed device, ware lid side-walls has the second friction sealed device;
Described tegmental wall comprises at least one aperture, and described aperture runs through tegmental wall.
7. closed culture dish according to claim 6, is characterized in that: also comprise apron or stopple or strainer, described apron or stopple or strainer removable be placed in described aperture, described stopple is provided with at least one second hole.
8. closed culture dish according to claim 6, is characterized in that: also comprise at least one and be positioned at first of described ware downside wall and grasp projection, or at least one is positioned at second of described ware lid sidewall and grasps projection.
9. closed culture dish according to claim 6, is characterized in that: also comprise the first projection that at least one is positioned at described diapire, or at least one is positioned at the second projection of described tegmental wall.
10. the closed culture dish according to claim 6 or 7, is characterized in that: at the bottom of described ware or being made up of rigid material at least partially of described ware lid, and described rigid material comprises glass, synthetic resins or corrosion resistant metal etc.; Described stopple at least partially by there is certain degree of hardness and elastic material forms, described in there is the cohort that certain degree of hardness and elastic material are selected from silica gel, rubber or other polymer materials combination composition.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104293657A (en) * 2014-11-10 2015-01-21 莆田学院 Sealed culture dish
CN104774753A (en) * 2015-04-25 2015-07-15 莆田学院 Surface sampling culture dish
CN106497768A (en) * 2016-11-30 2017-03-15 张家港市藏联生物研究所有限公司 A kind of Cordyceps militaris culture dish of sealing ventilation
CN106520534A (en) * 2016-11-30 2017-03-22 张家港市藏联生物研究所有限公司 Cordyceps militaris culture dish convenient to assemble and disassemble
CN106635765A (en) * 2016-11-07 2017-05-10 天津施特雷生物科技股份有限公司 Disposable sterile microbial culture dish
CN108559711A (en) * 2018-06-16 2018-09-21 福建省产品质量检验研究院 A kind of closed cultural method of mould

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104293657A (en) * 2014-11-10 2015-01-21 莆田学院 Sealed culture dish
CN104774753A (en) * 2015-04-25 2015-07-15 莆田学院 Surface sampling culture dish
CN106635765A (en) * 2016-11-07 2017-05-10 天津施特雷生物科技股份有限公司 Disposable sterile microbial culture dish
CN106497768A (en) * 2016-11-30 2017-03-15 张家港市藏联生物研究所有限公司 A kind of Cordyceps militaris culture dish of sealing ventilation
CN106520534A (en) * 2016-11-30 2017-03-22 张家港市藏联生物研究所有限公司 Cordyceps militaris culture dish convenient to assemble and disassemble
CN108559711A (en) * 2018-06-16 2018-09-21 福建省产品质量检验研究院 A kind of closed cultural method of mould
CN108559711B (en) * 2018-06-16 2021-05-04 福建省产品质量检验研究院 Closed type culture method of mould

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