CN204206905U - A kind of zebra fish anoxia experimental facility - Google Patents
A kind of zebra fish anoxia experimental facility Download PDFInfo
- Publication number
- CN204206905U CN204206905U CN201420468895.7U CN201420468895U CN204206905U CN 204206905 U CN204206905 U CN 204206905U CN 201420468895 U CN201420468895 U CN 201420468895U CN 204206905 U CN204206905 U CN 204206905U
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- nitrogen
- gas outlet
- dissolved oxygen
- zebra fish
- experimental facility
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The utility model discloses a kind of zebra fish anoxia experimental facility, comprise seal cover, dissolved oxygen meter, gas outlet, left side, left side air inlet, hard gauze, right side inlets, gas outlet, right side, nitrogen conduit, nitrogen pot, stirrer, casing; Seal cover is sealable, not only air admission hole and venthole make extraneous oxygen not easily enter, and save time but also save nitrogen; When the Bubbled stone of nitrogen conduit end makes to ventilate, bubble is unlikely to excessive; Near blow vent, porous barrier is set, bubble can be prevented the impact of fish, be convenient to different waters dissolved oxygen concentration simultaneously and arrive rapidly evenly; To water, nitrogen is passed into reduce dissolved oxygen concentration from 2 air admission holes; 2 ventholes connect dissolved oxygen meter probe and are convenient to monitor gas pressure intensity in oxygen in water concentration and stabilising arrangement at any time; Medicine in water can mix and reach the consistent of oxygen solubility by stirrer fast; Block is used for preventing adult fish or juvenile fish from swimming over to dividing plate external water area, is conducive to stability and the reliability of experimental result.
Description
Technical field
The utility model belongs to biomedicine experiment device field, particularly relates to a kind of zebra fish anoxia experimental facility.
Background technology
Anoxic plays an important role in embryonic development, physiology and pathological reaction, has relate at multiple ambit.
Zebra fish, owing to having plurality of advantages such as raising easily, embryo is transparent, in vitro fertilization, mutant species is many, genetic tool is ripe, has become the emerging model animal of research vertebrate animal development and human inheritance's disease.
Many scholars bring into use zebra fish to study anoxic in recent years, and the structure of anoxia model is generally by passing into nitrogen and measure oxygen in water concentration by dissolved oxygen meter in water, closed container again after requisite oxygen concentration to be achieved.This device has shortcomings:
1, cannot accomplish to seal completely during logical nitrogen, oxygen from water out while go back aerobic and enter from air, needs just can reach requisite oxygen concentration for a long time like this, and can waste a lot of nitrogen;
Have more bubble when 2, passing into nitrogen to produce, fish can be in stress situation in water, can produce certain impact to experimental result.
3, when carrying out chronic hypoxia research, zebra fish needs to survive the long period in the water of low DO concentration, and the oxygen concentration in water can change, and becomes unstable, makes result of study inaccurate and poor repeatability.
4, in the experiment needing pharmaceutical intervention, be difficult to make the drug concentration in whole container arrive evenly fast, larger impact can be produced on experimental result.
Utility model content
The purpose of this utility model is to provide a kind of zebra fish anoxia experimental facility, is intended to solve existing model and loses time to produce dysgenic problem with the oxygen concentration in more bubble, water when nitrogen, ventilation is wayward, drug concentration is uneven to result of study.
The utility model is achieved in that a kind of zebra fish anoxia experimental facility comprises seal cover, dissolved oxygen meter, gas outlet, left side, left side air inlet, hard gauze, right side inlets, gas outlet, right side, nitrogen conduit, nitrogen pot, stirrer, casing, dissolved oxygen meter and probe couple together through the gas outlet, left side a little more than the water surface by the connecting line of dissolved oxygen meter, probe inserts below the water surface, close to the hard gauze of bottom, nitrogen conduit enters in water through left side air inlet and right side inlets, the outlet of nitrogen conduit is positioned at bottom half, the left side of casing, right side, bottom is respectively placed with one piece of dividing plate, all with hard gauze in every block dividing plate, gas outlet, right side controls for casing internal gas pressure, can close or open at any time, nitrogen conduit diameter is slightly less than 10mm and by with triple valve 2 nitrogen conduits and nitrogen pot being coupled together the pneumatic control that works of going forward side by side, stirrer is positioned at bottom half, immediately below bottom baffles, band is magnetic, the agitation speed of stirrer is controlled by magnetic stirring apparatus.
Further, the lateral surface distance body side of left and right side dividing plate is about 20mm, and bottom outside identity distance is about 30mm from bottom half, 3 pieces of dividing plate medial surfaces are all with hard gauze, hard gauze is divided into two kinds of specifications according to the aperture of mesh, large-mesh diameter d=3mm, fine mesh diameter d=0.5mm.
Further, on the left of casing, the diameter of gas outlet, left side air inlet, gas outlet, right side, right side inlets is 10mm.
Further, nitrogen conduit end is provided with Bubbled stone.
The beneficial effects of the utility model are as follows:
1, seal cover can seal after putting into zebra fish, not only only stay 2 air admission holes and 2 ventholes, extraneous oxygen can not be entered, and nitrogen is unlikely to too fast spilling, saves time but also can save nitrogen.
2, add Bubbled stone at the catheter tip passing into nitrogen, when making to ventilate, bubble is unlikely to excessive; At blow vent, place arranges baffle plate, makes fish can not be movable with from its impact around it.
3, zebra fish anoxia experimental facility passes into nitrogen by three-way pipe, and one end connects nitrogen valve, and two ends pass into nitrogen from 2 ventilation holes in addition, separately establish two ventholes, after measured, so water oxygen concentration stabilize in energy holding device, and gas pressure intensity in energy stabilising arrangement.
4, zebra fish anoxia experimental facility is divided into dividing plate and can be placed stirrer, can by medicine mixing in water under the effect of magnetic stirring apparatus.
Accompanying drawing explanation
Fig. 1 is the structural representation of the zebra fish anoxia experimental facility that the utility model embodiment provides;
In figure: 1, seal cover; 2, dissolved oxygen meter; 3, gas outlet, left side; 4, left side air inlet; 5, hard gauze; 6, right side inlets; 7, gas outlet, right side; 8, nitrogen conduit; 9, nitrogen pot; 10, stirrer; 11, casing.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
Fig. 1 shows the structure of zebra fish anoxia experimental facility of the present utility model, as shown in the figure, the utility model is achieved in that a kind of zebra fish anoxia experimental facility comprises seal cover 1, dissolved oxygen meter 2, gas outlet, left side 3, left side air inlet 4, hard gauze 5, right side inlets 6, gas outlet, right side 7, nitrogen conduit 8, nitrogen pot 9, stirrer 10, casing 11, dissolved oxygen meter 2 and probe couple together through the gas outlet, left side 3 a little more than the water surface by the connecting line of dissolved oxygen meter 2, probe inserts below the water surface, close to the hard gauze 5 of bottom, nitrogen conduit 8 enters in water through left side air inlet 4 and right side inlets 6, the outlet of nitrogen conduit 8 is positioned at bottom casing 11, the left side of casing 11, right side, bottom is respectively placed with one piece of dividing plate, all with hard gauze in every block dividing plate, gas outlet 7, right side controls for casing 11 internal gas pressure, can close or open at any time, nitrogen conduit 8 diameter is slightly less than 10mm and by with triple valve 2 nitrogen conduits and nitrogen pot 9 being coupled together the pneumatic control that works of going forward side by side, stirrer 10 is positioned at bottom casing 11, immediately below bottom baffles, band is magnetic, the agitation speed of stirrer 10 is controlled by magnetic stirring apparatus.
Further, the lateral surface of left and right side dividing plate distance casing 11 side is about 20mm, and bottom outside identity distance is from about 30mm bottom casing 11,3 pieces of dividing plate medial surfaces are all with hard gauze, hard gauze is divided into two kinds of specifications according to the aperture of mesh, large-mesh diameter d=3mm, fine mesh diameter d=0.5mm.
Further, the diameter of gas outlet, left side 3, left side air inlet 4, gas outlet, right side 6, right side inlets 7 is 10mm.
Further, nitrogen conduit 8 end is provided with Bubbled stone.
Operation principle of the present utility model: this zebra fish anoxia experimental facility seal cover 1 can seal after putting into zebra fish, only in side, 2 air admission holes and 2 ventholes are stayed in nearly top, extraneous oxygen is not easily entered, make the nitrogen of hypoxemia in maintenance experimental provision be unlikely to too fast spilling simultaneously, not only save time but also save nitrogen; Add Bubbled stone at the catheter tip passing into nitrogen, when making to ventilate, bubble is unlikely to excessive, causes shade in order to avoid cause the water surface to agitate thus affects animal behavioral study; Porous barrier is set near blow vent, bubble can be prevented the impact of fish, be convenient to different waters dissolved oxygen concentration simultaneously and arrive rapidly evenly; The utility model passes into nitrogen through triple valve, and one end is through tubes connection nitrogen pot valve, and two ends connecting duct passes into nitrogen to reduce dissolved oxygen concentration from 2 air admission holes to water in addition; If 2 ventholes, connect dissolved oxygen meter probe by venthole on the one hand, monitor oxygen in water concentration at any time, retain gas pressure intensity in venthole energy stabilising arrangement on the other hand; Be provided with lower floor's diaphragm plate in the utility model device, under dividing plate, place stirrer, can fast medicine in water be mixed and reach the consistent of oxygen solubility under the effect of magnetic stirring apparatus; The medial surface of diaphragm plate and vertical clapboard can insert the block of different pore size, be respectively used to prevent adult fish or juvenile fish from swimming over to dividing plate external water area, be convenient to observe in the behaviouristics of restriction waters to fish, and avoid fish be subject to the impact of higher concentration medicine in bottom waters and near Bubbled stone, be subject to the impact of higher oxygen concentration, be conducive to stability and the reliability of experimental result.
By reference to the accompanying drawings embodiment of the present utility model is described although above-mentioned; but the restriction not to the utility model protection domain; one of ordinary skill in the art should be understood that; on the basis of the technical solution of the utility model, those skilled in the art do not need to pay various amendment or distortion that performing creative labour can make still within protection domain of the present utility model.
Claims (4)
1. a zebra fish anoxia experimental facility, it is characterized in that, this zebra fish anoxia experimental facility comprises seal cover, dissolved oxygen meter, gas outlet, left side, left side air inlet, hard gauze, right side inlets, gas outlet, right side, nitrogen conduit, nitrogen pot, stirrer, casing, dissolved oxygen meter and probe couple together through the gas outlet, left side a little more than the water surface by the connecting line of dissolved oxygen meter, probe inserts below the water surface, close to the hard gauze of bottom, nitrogen conduit enters in water through left side air inlet and right side inlets, the outlet of nitrogen conduit is positioned at bottom half, the left side of casing, right side, bottom is respectively placed with one piece of dividing plate, all with hard gauze in every block dividing plate, gas outlet, right side controls for casing internal gas pressure, can close or open at any time, nitrogen conduit diameter is slightly less than 10mm and by with triple valve 2 nitrogen conduits and nitrogen pot being coupled together the pneumatic control that works of going forward side by side, stirrer is positioned at bottom half, immediately below bottom baffles, band is magnetic, the agitation speed of stirrer is controlled by magnetic stirring apparatus.
2. zebra fish anoxia experimental facility as claimed in claim 1, it is characterized in that, the lateral surface distance body side of left and right side dividing plate is about 20mm, bottom outside identity distance is about 30mm from bottom half, 3 pieces of dividing plate medial surfaces are all with hard gauze, hard gauze is divided into two kinds of specifications according to the aperture of mesh, large-mesh diameter d=3mm, fine mesh diameter d=0.5mm.
3. zebra fish anoxia experimental facility as claimed in claim 1, it is characterized in that, on the left of casing, the diameter of gas outlet, left side air inlet, gas outlet, right side, right side inlets is 10mm.
4. zebra fish anoxia experimental facility as claimed in claim 1, it is characterized in that, nitrogen conduit end is provided with Bubbled stone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420468895.7U CN204206905U (en) | 2014-08-19 | 2014-08-19 | A kind of zebra fish anoxia experimental facility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420468895.7U CN204206905U (en) | 2014-08-19 | 2014-08-19 | A kind of zebra fish anoxia experimental facility |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204206905U true CN204206905U (en) | 2015-03-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420468895.7U Expired - Fee Related CN204206905U (en) | 2014-08-19 | 2014-08-19 | A kind of zebra fish anoxia experimental facility |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204206905U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111096255A (en) * | 2019-12-31 | 2020-05-05 | 天津市胸科医院 | Non-operative intervention fish artificial lung system and application thereof |
| CN115250971A (en) * | 2022-07-13 | 2022-11-01 | 山东省科学院生物研究所 | Method for establishing zebra fish myocardial hypoxia model and application |
-
2014
- 2014-08-19 CN CN201420468895.7U patent/CN204206905U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111096255A (en) * | 2019-12-31 | 2020-05-05 | 天津市胸科医院 | Non-operative intervention fish artificial lung system and application thereof |
| CN115250971A (en) * | 2022-07-13 | 2022-11-01 | 山东省科学院生物研究所 | Method for establishing zebra fish myocardial hypoxia model and application |
| CN115250971B (en) * | 2022-07-13 | 2023-07-14 | 山东省科学院生物研究所 | Method for establishing zebra fish myocardial anoxia model and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20170819 |