CN204188496U - A kind of laser confocal microscope - Google Patents
A kind of laser confocal microscope Download PDFInfo
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- CN204188496U CN204188496U CN201420717688.0U CN201420717688U CN204188496U CN 204188496 U CN204188496 U CN 204188496U CN 201420717688 U CN201420717688 U CN 201420717688U CN 204188496 U CN204188496 U CN 204188496U
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Abstract
The utility model discloses a kind of laser confocal microscope, comprise laser instrument, microscope optical system and control system, described laser instrument is positioned at the side of microscope optical system, described microscope optical system comprises detecting device from top to bottom successively, confocal pinhole grating, barrier filter, spectroscope and scanister, the light source irradiation that described laser instrument sends is on spectroscope, the front end of laser instrument is provided with exciter filter, below described microscope optical system, object lens are installed, below object lens, sample stage is installed, the progressive motor of trace is installed bottom described sample stage, described control system comprises image storage system and scanning control system, described image storage system is connected with detecting device, scanning control system is connected with scanister.The utility model has the features such as high resolving power, high sensitivity, high power, can do several functions on a cellular level and measure and analyze, really can be had the primary colour image of three-dimensional sharpness.
Description
[technical field]
The utility model relates to microscopical technical field, particularly a kind of technical field of laser confocal microscope.
[background technology]
Microscope is a kind of optical instrument be made up of the combination of lens or several lens, is that the mankind enter atom-age mark, is mainly used in amplifying that small items becomes is the instrument that the naked eyes of people can be seen.Microscope is presented in an a whole new world in the visual field of the mankind.People see hundreds of " new " animalcule and plant first time, and the internal structure of various thing such as from human body to vegetable fibre.Microscope also contributes to scientist and finds new species, contributes to medical treatment disease.But traditional optical microscope uses a light source, on sample, the image of every bit all can be subject to the diffraction light of neighbor point or the interference of scattered light, and microscopical imaging effect has much room for improvement.
[utility model content]
The purpose of this utility model solves the problems of the prior art exactly, a kind of laser confocal microscope is proposed, compared with conventional microscope, there is the features such as high resolving power, high sensitivity, high power, several functions can be done on a cellular level measure and analyze, really can be had the primary colour image of three-dimensional sharpness, laser scanning co-focusing microscope can process sample alive simultaneously, can not cause the destruction of physicochemical characteristics to sample.
For achieving the above object, the utility model proposes a kind of laser confocal microscope, comprise laser instrument, microscope optical system and control system, described laser instrument is positioned at the side of microscope optical system, described microscope optical system comprises detecting device from top to bottom successively, confocal pinhole grating, barrier filter, spectroscope and scanister, the light source irradiation that described laser instrument sends is on spectroscope, the front end of laser instrument is provided with exciter filter, below described microscope optical system, object lens are installed, below object lens, sample stage is installed, the progressive motor of trace is installed bottom described sample stage, described control system comprises image storage system and scanning control system, described image storage system is connected with detecting device, scanning control system is connected with scanister.
As preferably, described laser instrument adopt in Argon ion laser, He-Ne green laser, the red laser instrument of He-Ne and semiconductor laser any one, described object lens adopt high-NA objective, and the numerical aperture of object lens is 1.3 ~ 1.5.
As preferably, described detecting device adopts low noise photomultiplier, and be provided with reflective mirror below described sample stage, the side of described reflective mirror is provided with photomultiplier.
As preferably, described image storage system is connected with video display devices, printing equipment and three-dimensional reconstruction apparatus.
The beneficial effects of the utility model: the utility model is compared with conventional microscope, there is the features such as high resolving power, high sensitivity, high power, several functions can be done on a cellular level measure and analyze, really can be had the primary colour image of three-dimensional sharpness, laser scanning co-focusing microscope can process sample alive simultaneously, can not cause the destruction of physicochemical characteristics to sample.
Feature of the present utility model and advantage will be described in detail by reference to the accompanying drawings by embodiment.
[accompanying drawing explanation]
Fig. 1 is the structural representation of a kind of laser confocal microscope of the utility model.
[embodiment]
Consult Fig. 1, a kind of laser confocal microscope of the utility model, comprise laser instrument 1, microscope optical system 2 and control system 3, described laser instrument 1 is positioned at the side of microscope optical system 2, described microscope optical system 2 comprises detecting device 21 from top to bottom successively, confocal pinhole grating 22, barrier filter 23, spectroscope 24 and scanister 25, the light source irradiation that described laser instrument 1 sends is on spectroscope 24, the front end of laser instrument 1 is provided with exciter filter 11, below described microscope optical system 2, object lens 4 are installed, sample stage 5 is installed below object lens 4, the progressive motor 53 of trace is installed bottom described sample stage, described control system 3 comprises image storage system 31 and scanning control system 32, described image storage system 31 is connected with detecting device 21, scanning control system 32 is connected with scanister 25.
Described laser instrument 1 adopt in Argon ion laser, He-Ne green laser, the red laser instrument of He-Ne and semiconductor laser any one, described object lens 4 adopt high-NA objective, the numerical aperture of object lens 4 is 1.3 ~ 1.5, described detecting device 21 adopts low noise photomultiplier, below described sample stage 5, reflective mirror 51 is installed, the side of described reflective mirror 51 is provided with photomultiplier 52, described image storage system 31 is connected with video display devices 33, printing equipment 34 and three-dimensional reconstruction apparatus 35.
Utility model works process:
A kind of laser confocal microscope of the utility model in the course of the work, after selecting suitable excitation wavelength, laser instrument 1 is selected, Argon ion laser: 457nm, 477nm, 488nm, 514nm, He-Ne green laser: 543nm, the red laser instrument of He-Ne: 633nm, semiconductor laser: 405nm, then suitable spectroscope 24 and exciter filter 11 is selected, 3-D scanning mode is adopted to scan, determine scanning density (resolution): 256 × 256, 512 × 512, 1024 × 1024, 2048 × 2048, choose the multiple of object lens 4, after this condition is determined, namely sweep limit is determined, the light transmission of object lens 4 is directly proportional to 4 powers of numerical aperture (NA), with square being inversely proportional to of the enlargement factor of object lens, therefore, the object lens of high-NA should be selected as far as possible.
The every bit of the laser beam that laser confocal microscope utilizes laser instrument 1 to send in scanister 25 pairs of samples on focal plane scans, the fluorescence of the illuminated transmitting on sample is in confocal pinhole grating 22 place's imaging, and all stopped by barrier filter 23 from any emitting fluorescence beyond this point, accepted, by image storage system 31 imaging on video display devices 33 by detecting device 21 pointwise above confocal pinhole grating 22.Sample stage 5 adds a micro-step motor 53, make sample stage 5 vertically (Z axis) up and down stepping move, new for sample aspect is moved on on focal plane, this aspect is imaged on again on video display devices 33, along with the continuous movement of Z axis, just can obtain sample different aspects continuous print optical imagery, realize the object of " optical section ", be called visually " Micro-CT scanning ", obtained the primary colour image of three-dimensional sharpness on this basis by three-dimensional reconstruction apparatus 35, image prints by printing equipment 34.
The utility model is compared with conventional microscope, there is the features such as high resolving power, high sensitivity, high power, several functions can be done on a cellular level measure and analyze, really can be had the primary colour image of three-dimensional sharpness, laser scanning co-focusing microscope can process sample alive simultaneously, can not cause the destruction of physicochemical characteristics to sample.
Above-described embodiment is to explanation of the present utility model, is not to restriction of the present utility model, anyly all belongs to protection domain of the present utility model to the scheme after the utility model simple transformation.
Claims (4)
1. a laser confocal microscope, it is characterized in that: comprise laser instrument (1), microscope optical system (2) and control system (3), described laser instrument (1) is positioned at the side of microscope optical system (2), described microscope optical system (2) comprises detecting device (21) from top to bottom successively, confocal pinhole grating (22), barrier filter (23), spectroscope (24) and scanister (25), the light source irradiation that described laser instrument (1) sends is on spectroscope (24), the front end of laser instrument (1) is provided with exciter filter (11), described microscope optical system (2) below is provided with object lens (4), object lens (4) below is provided with sample stage (5), the progressive motor (53) of trace is installed bottom described sample stage, described control system (3) comprises image storage system (31) and scanning control system (32), described image storage system (31) is connected with detecting device (21), scanning control system (32) is connected with scanister (25).
2. a kind of laser confocal microscope as claimed in claim 1, it is characterized in that: described laser instrument (1) adopt in Argon ion laser, He-Ne green laser, the red laser instrument of He-Ne and semiconductor laser any one, described object lens (4) adopt high-NA objective, and the numerical aperture of object lens (4) is 1.3 ~ 1.5.
3. a kind of laser confocal microscope as claimed in claim 1, it is characterized in that: described detecting device (21) adopts low noise photomultiplier, described sample stage (5) below is provided with reflective mirror (51), and the side of described reflective mirror (51) is provided with photomultiplier (52).
4. a kind of laser confocal microscope as claimed in claim 1, is characterized in that: described image storage system (31) is connected with video display devices (33), printing equipment (34) and three-dimensional reconstruction apparatus (35).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420717688.0U CN204188496U (en) | 2014-11-19 | 2014-11-19 | A kind of laser confocal microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420717688.0U CN204188496U (en) | 2014-11-19 | 2014-11-19 | A kind of laser confocal microscope |
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| Publication Number | Publication Date |
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| CN204188496U true CN204188496U (en) | 2015-03-04 |
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| CN201420717688.0U Expired - Fee Related CN204188496U (en) | 2014-11-19 | 2014-11-19 | A kind of laser confocal microscope |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568755A (en) * | 2016-11-06 | 2017-04-19 | 浙江大学 | Near infrared laser scanning confocal microscopic imaging system |
| CN108535671A (en) * | 2018-04-24 | 2018-09-14 | 金华职业技术学院 | Method for measuring nanoscale magnetization dynamics |
| CN108957371A (en) * | 2018-04-24 | 2018-12-07 | 金华职业技术学院 | A kind of thin film magnetic measurement method |
| CN110058004A (en) * | 2019-04-11 | 2019-07-26 | 高权 | A kind of experimental provision of laser co-focusing detection electro photoluminescence safety |
| CN112162398A (en) * | 2020-10-23 | 2021-01-01 | 南京大学 | Portable intelligent interconnected optical microscope system |
-
2014
- 2014-11-19 CN CN201420717688.0U patent/CN204188496U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568755A (en) * | 2016-11-06 | 2017-04-19 | 浙江大学 | Near infrared laser scanning confocal microscopic imaging system |
| CN108535671A (en) * | 2018-04-24 | 2018-09-14 | 金华职业技术学院 | Method for measuring nanoscale magnetization dynamics |
| CN108957371A (en) * | 2018-04-24 | 2018-12-07 | 金华职业技术学院 | A kind of thin film magnetic measurement method |
| CN108535671B (en) * | 2018-04-24 | 2020-08-28 | 金华职业技术学院 | Method for measuring nanoscale magnetization dynamics |
| CN110058004A (en) * | 2019-04-11 | 2019-07-26 | 高权 | A kind of experimental provision of laser co-focusing detection electro photoluminescence safety |
| CN112162398A (en) * | 2020-10-23 | 2021-01-01 | 南京大学 | Portable intelligent interconnected optical microscope system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20151119 |
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| EXPY | Termination of patent right or utility model |