CN204188440U - Cell mass gatherer - Google Patents
Cell mass gatherer Download PDFInfo
- Publication number
- CN204188440U CN204188440U CN201420695555.8U CN201420695555U CN204188440U CN 204188440 U CN204188440 U CN 204188440U CN 201420695555 U CN201420695555 U CN 201420695555U CN 204188440 U CN204188440 U CN 204188440U
- Authority
- CN
- China
- Prior art keywords
- centrifuge tube
- conical section
- gatherer
- stay pipe
- filter paper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model provides a kind of cell mass gatherer, comprise the first centrifuge tube, the stay pipe of tubular is provided with in first centrifuge tube, be provided with lower end in stay pipe and stretch into the second centrifuge tube in the first centrifuge tube conical section, the conical section of the second centrifuge tube is provided with multiple fluid hole, during use, in the conical section of the second centrifuge tube, put into filter paper, the inwall of filter paper and this conical section is fitted.This gatherer censorship sample can be fixed after tissue carry out whole embedded section, serial section, improve positive rate, overcome the phenomenon that cell overlap accumulation in smear, uneven distribution, structure are unclear, information is more accurately provided.
Description
Technical field
The utility model belongs to basic medical research technical field, relates to a kind of device collecting cell in humoral specimen, particularly a kind of cell mass gatherer.
Background technology
Traditional examination of castoff cells, the humoral specimens such as chest, ascites, urine that take a morsel exactly carry out sample smear or centrifugation, and take a morsel smear, therefrom searches tumour cell, have simple, the economic and painful advantage such as few of method and are applied by clinical.But its shortcoming is: after chest, ascites, urine are centrifugal, only gets its a little sediment and carry out smear, sample all can not be made smear and observe, easily cause and fail to pinpoint a disease in diagnosis and mistaken diagnosis, cell smear is thicker simultaneously, when observer lacks experience, easy mistaken diagnosis, particularly easily causes false positive.
Summary of the invention
The purpose of this utility model is to provide a kind of cell mass gatherer, can collect the whole cells in a small amount of humoral specimen, not easily cause and fail to pinpoint a disease in diagnosis and mistaken diagnosis.
For achieving the above object, the technical scheme that the utility model adopts is: a kind of cell mass gatherer, comprise the first centrifuge tube, the stay pipe of tubular is provided with in first centrifuge tube, the second centrifuge tube is provided with in stay pipe, conical section on second centrifuge tube stretches in the conical section of the first centrifuge tube through stay pipe, the sidewall of the second centrifuge tube conical section is provided with multiple fluid hole, during use, filter paper is put into the conical section of the second centrifuge tube, and the inwall of filter paper and this conical section is fitted.
Collect cast-off cells making specimens paraffin embedding slices with the utility model gatherer to have the following advantages:
1) tissue after the cast-off cells samples such as the chest of censorship, ascites, urine can being fixed carries out whole embedded section, and can serial section, and minimizing is failed to pinpoint a disease in diagnosis, and improves the positive rate of cast-off cells.
2) overcome the phenomenon that cell overlap accumulation in smear, uneven distribution, structure are unclear, information is more accurately provided.
3) specimens paraffin embedding slices made can be preserved for a long time, repeatedly heavily cuts, and can do multiple specific stain or SABC, is very helpful to the classification of malignant tumour, reduces mistaken diagnosis, for clinical treatment provides foundation and convenience.
4) by the restriction of not antifreeze, anticorrosion, the time length of cytolgical examination, can stay in the bottle with immobile liquid at any time, the amount of leaving and taking of sample also can suitably increase.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model gatherer.
In Fig. 1: 1. the first centrifuge tube, 2. the second instrumentation tubes, 3. stay pipe, 4. fluid hole.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in detail.
As shown in Figure 1, the utility model gatherer, comprise the first centrifuge tube 1, the stay pipe 3 of tubular is provided with in first centrifuge tube 1, the lower end of stay pipe 3 contacts with the cylindrical tube of the first centrifuge tube 1 and the joint portion of conical section, the conical section be provided with in stay pipe 3 on second centrifuge tube 2, second centrifuge tube 2 stretches in the conical section of the first centrifuge tube 1 through stay pipe 3, and the sidewall of the second centrifuge tube 2 conical section is provided with multiple fluid hole 4.
When using the utility model gatherer, filter paper is put into the conical section of the second centrifuge tube 2, and the inwall of filter paper and this conical section is fitted.
The utility model additionally provides a kind of method utilizing above-mentioned gatherer to collect cell in a small amount of humoral specimen, specifically carries out according to the following steps:
Step 1: 1 ︰ 9 by volume, added in 200 ~ 800mL humoral specimen by the formalin 20 ~ 80ml of massfraction 35 ~ 40%, sample is fixed, then natural subsidence 30 ~ 60min, abandoning supernatant, stays sediment;
Filter paper is put into the conical section of the second centrifuge tube 2, and the inwall of filter paper and this conical section is fitted;
Step 2: sediment 30 ~ 50mL is injected centrifuge tube, with the centrifugal 10min of the rotating speed of 1500 ~ 2000r/min, abandoning supernatant, taking precipitate 1 ~ 2 carries out routine smear, in remaining sediment, add the ethanol of 1mL massfraction 95%, after mixing, add 1 protein chelates agent, mixing, obtains suspension;
This protein chelates agent is egg, consumption 1, about 50 μ L, and its consumption can not be too much, otherwise affect cell quantity in cell mass.Make egg white sex change by ethanol, be frozen into lumps.
Step 3: suspension is filled on the filter paper in the second centrifuge tube, with the centrifugal 10min of the rotating speed of 1500 ~ 2000r/min, the clear liquid in suspension, through filter paper, enters in the first centrifuge tube 1 by fluid hole 4, solid granule in suspension is trapped on filter paper, forms cell mass;
Step 4: cell mass undertaken embedding by existing method, dewaters, embed after transparent and waxdip, make the cell lump paraffin section of thickness 4 μm, after carrying out HE dyeing, can observe.
The hydro-extractor used in the utility model collection method is consistent with conventional cell smear hydro-extractor used.
Claims (2)
1. a cell mass gatherer, it is characterized in that, comprise the first centrifuge tube (1), the stay pipe (3) of tubular is provided with in first centrifuge tube (1), the second centrifuge tube (2) is provided with in stay pipe (3), conical section on second centrifuge tube (2) stretches in the conical section of the first centrifuge tube (1) through stay pipe (3), the sidewall of the second centrifuge tube (2) conical section is provided with multiple fluid hole (4), during use, filter paper is put into the conical section of the second centrifuge tube (2), and the inwall of filter paper and this conical section is fitted.
2. cell mass gatherer according to claim 1, is characterized in that, the lower end of stay pipe (3) contacts with the cylindrical tube of the first centrifuge tube (1) and the joint portion of conical section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420695555.8U CN204188440U (en) | 2014-11-19 | 2014-11-19 | Cell mass gatherer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420695555.8U CN204188440U (en) | 2014-11-19 | 2014-11-19 | Cell mass gatherer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204188440U true CN204188440U (en) | 2015-03-04 |
Family
ID=52620423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420695555.8U Expired - Fee Related CN204188440U (en) | 2014-11-19 | 2014-11-19 | Cell mass gatherer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204188440U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
-
2014
- 2014-11-19 CN CN201420695555.8U patent/CN204188440U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104359746A (en) * | 2014-11-19 | 2015-02-18 | 白银市第一人民医院 | Cell mass collector and method for collecting cells in liquid specimen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20151119 |
|
| EXPY | Termination of patent right or utility model |