CN204143278U - Real-time temperature control minisize sample cell - Google Patents

Real-time temperature control minisize sample cell Download PDF

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Publication number
CN204143278U
CN204143278U CN201420591122.8U CN201420591122U CN204143278U CN 204143278 U CN204143278 U CN 204143278U CN 201420591122 U CN201420591122 U CN 201420591122U CN 204143278 U CN204143278 U CN 204143278U
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sample chamber
temperature control
tec
support plate
temperature
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CN201420591122.8U
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Chinese (zh)
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李迪
李银妹
呼新尧
周金华
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University of Science and Technology of China USTC
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University of Science and Technology of China USTC
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Abstract

本实用新型提出一种实时温控微型样品室,包括控温装置(A)、样品室(B)和控温仪表(C)三部分。控温装置(A)包括U型支撑板(①)、半导体制冷片(TEC)(②)、TEC的金属导热片(③)、热电偶探头(④)、玻片支撑板(⑤)和隔热板(⑥)。样品室(B)包括上盖玻片(⑦)、样品室基板(⑧)和下盖玻片(⑨)。控温仪表(C)包括控温电源、反馈控制器和导线。本实用新型可对样品的温度进行实时控制和调节,温控范围为6-90℃,温度响应时间≤3min,温度控制精度为±0.5℃。样品室不会引入任何机械振动,无噪声源;容积可调,最少仅需10μl溶液即可充满样品室;体积小,特别满足由于高倍率物镜导致的显微镜载物台空间十分有限的情况。

The utility model proposes a real-time temperature-controlled miniature sample room, which includes three parts: a temperature control device (A), a sample room (B) and a temperature control instrument (C). The temperature control device (A) includes a U-shaped support plate (①), a semiconductor cooling chip (TEC) (②), a metal heat conduction sheet of the TEC (③), a thermocouple probe (④), a glass slide support plate (⑤) and a spacer. Hot plate (⑥). The sample chamber (B) includes an upper cover glass (⑦), a sample chamber substrate (⑧) and a lower cover glass (⑨). The temperature control instrument (C) includes a temperature control power supply, a feedback controller and wires. The utility model can control and adjust the temperature of the sample in real time, the temperature control range is 6-90°C, the temperature response time is ≤3min, and the temperature control accuracy is ±0.5°C. The sample chamber does not introduce any mechanical vibration and has no noise source; the volume is adjustable, and only 10 μl of solution is needed to fill the sample chamber; the small size is especially suitable for the situation where the space of the microscope stage is very limited due to the high magnification objective lens.

Description

Real-time temperature control minisize sample cell
Technical field
The utility model belongs to micro-container technical field, be specifically related to a kind of can the minisize sample cell of real-time implementation temperature adjusting.
Background technology
In microexamination experiment and micromanipulation experiment, common sample chamber, owing to not having temp regulating function, can only at room temperature be tested.But in actual applications, a lot of biological, physics and chemical experiment need to maintain specified temp (not necessarily room temperature) in experimentation, or carrying out and change temperature with experiment, therefore need to install the sample chamber with temperature control device additional.
The general volume of current existing sample chamber attemperating unit is comparatively large, and multi-band has the noise sources such as fan, cannot meet and use the narrow working space of high magnification microcobjective and the ultra-low noise requirement needed for high-acruracy survey.
Utility model content
The purpose of this utility model is: provide a kind of minisize sample cell that can realize real time temperature regulation and control, in experimentation, the temperature of sample indoor liquid controlled in real time and regulate, sample chamber volume is little, do not introduce any mechanical vibration, have preferably temperature-time responding ability and temperature degree of regulation.
The technical solution adopted in the utility model is: a kind of real-time temperature control minisize sample cell, comprises temperature regulating device A, sample chamber B and temperature control instrument C, wherein:
1. temperature regulating device A comprises U-shaped back up pad, semiconductor chilling plate (TEC) 2., the metal conducting strip of TEC 3., thermocouple probe 4., slide support plate 5. with thermal insulation board 6.;
The U-shaped back up pad of temperature regulating device A 1. down either side be two semiconductor chilling plates (TEC) 2., 3. each semiconductor chilling plate (TEC) 2. below is all equipped with the metal conducting strip of TEC, 5. the metal conducting strip of two TEC is 3. common below connects slide support plate, slide support plate be 5. below two thermal insulation boards 6., Open Side Down for the square groove of the metal conducting strip (3.) of TEC, in the metal conducting strip that 4. two thermocouple probes are arranged on two TEC respectively square groove 3., and make thermocouple probe 4. TEC metal conducting strip 3. and slide support plate 5. between.
1., 2., the metal conducting strip of TEC 3. for semiconductor chilling plate (TEC) for U-shaped back up pad, thermocouple probe 4., 5. slide support plate uses heat-conducting glue to bond, and is then 6. used by thermal insulation board common adhesive to be bonded in slide support plate below 5., final composition temperature regulating device A.
7. sample chamber B comprises cover glass, sample chamber substrate 8. with lower cover glass 9., 8. sample chamber substrate is the sheet metal with square hole.
By upper cover glass 7., 9. 8. sample chamber substrate bond with 502 quick-drying gelatins in turn with lower cover glass, from forming sample chamber B, due to the sheet metal that 8. sample chamber substrate is with square hole, therefore understand after bonding and 7. 9. form the cavity of a strip in centre with lower cover glass at upper cover glass, and an aperture is left respectively in both sides, can be used for application of sample and exhaust.
Temperature control instrument C comprises temperature-controlled power, feedback controller and wire.
Temperature regulating device A and sample chamber B use transparent adhesive tape to be connected, and semiconductor chilling plate (TEC) wire 2. and thermocouple probe wire 4. need be connected on the feedback controller of temperature control instrument C.
Further, sample chamber volume can be changed by changing sample chamber substrate thickness 8..
Further, connect with heat-conducting glue between all parts in temperature regulating device A, ensure thermal conduction capability and physical strength, and ensure that 3. 1. U-shaped back up pad directly do not contact with the metal conducting strip of TEC, reduce the heat transfer between semiconductor chilling plate (TEC) cold and hot surface 2..
The utility model has the advantage of:
1. the utility model controls in real time the temperature in sample chamber and regulates in experimentation, has preferably temperature-time responding ability and temperature degree of regulation.
2. the utility model sample chamber volume is little, and practicality is wide, meets the situation that the microscope stage space that causes due to high magnification object lens is very limited especially.
3. the utility model does not introduce any mechanical vibration, noiseless source, can be used for high-precision measurement.
4. the internal adjustable volume of sample chamber (B) in the utility model, the minimum 10 μ l solution that only need can be full of sample chamber when 8. thickness is 0.1mm (sample chamber substrate).
Accompanying drawing explanation
Fig. 1 is that the utility model is a kind of can the minisize sample cell structural representation of real-time implementation temperature adjusting, and in figure, numbering is followed successively by:
1. be U-shaped back up pad,
2. be TEC (semiconductor chilling plate),
3. be the metal conducting strip of TEC,
4. be thermocouple probe,
5. be slide support plate,
6. be thermal insulation board,
7. be upper cover glass,
8. be sample chamber substrate,
9. be lower cover glass;
Fig. 2 is can the minisize sample cell finished product schematic diagram of real-time implementation temperature adjusting;
Fig. 3 be U-shaped back up pad 1., wherein scheme (a) be U-shaped back up pad vertical view, figure (b) be U-shaped back up pad side view; Unit: mm;
Fig. 4 be the metal conducting strip of TEC 3., wherein to scheme (a) be TEC metal conducting strip vertical view, the metal conducting strip side view that figure (b) is TEC; Unit: mm;
Fig. 5 be slide support plate 5., 75mm × 25mm × 1mm;
Fig. 6 be sample chamber substrate 8., 32mm × 22mm × 0.1mm (0.1mm is substrate thickness, and its value is optional).
Embodiment
The utility model is further illustrated below in conjunction with accompanying drawing and specific embodiment.
The utility model proposes a kind of can the minisize sample cell of real-time implementation temperature adjusting.The structure of this minisize sample cell is made up of temperature regulating device A, sample chamber B and temperature control instrument C tri-part.As shown in Figure 1, the real-time temperature control minisize sample cell be combined to form is the pattern of Fig. 2 to the structure of temperature regulating device A and sample chamber B.
Temperature regulating device A: by element 1.-5. use heat-conducting glue be bonded, 6. use 502 quick-drying gelatins to stick to 5., as shown in Figure 2.U-shaped back up pad is 1.: external dimensions 90mm × 40mm × 3mm, side symcenter is processed into the square groove of 50mm × 30mm.The one side use heat-conducting glue that U-shaped portion is divided and TEC 2. (20mm × 20mm × 3mm, model tes1-04902) link.The selection of TEC top and bottom 2. will coordinate with the both positive and negative polarity of ambient controller, connects and instead can cannot realize temperature adjusting.3. TEC one side is 2. connected with the metal conducting strip of TEC by heat-conducting glue.The metal conducting strip of TEC is 3.: external dimensions 30mm × 25mm × 5mm, has 3mm × 3mm square groove, as shown in Figure 4.In the metal conducting strip that 4. thermocouple probe is fixed on TEC by heat-conducting glue groove 3..Finally use heat-conducting glue or 502 quick-drying gelatins respectively by slide support plate 5. and thermal insulation board be 6. fixed on the metal conducting strip 3. below of TEC successively.Slide support plate 5. external dimensions 75mm × 25mm, Central Symmetry is processed into 22mm × 19mm square groove, and thickness is 1mm, as shown in Figure 5.
Sample chamber B: by element 7.-9. use 502 quick-drying gelatins to be bonded.Cover glass 7. (sailing boat board) apparent size is 18mm × 18mm × 0.17mm; Lower cover glass size 9. (Corning Cover Glass Cat.No.2865-22) is 22mm × 22mm × 0.17mm; Sample chamber substrate is 8.: scale copper, outside dimension 32mm × 22mm × 0.1mm, 22mm × 4mm square hole is processed at center, as shown in Figure 6.Sample chamber substrate thickness is optional, if use 0.1mm thin slice, then sample chamber internal volume about 10 μ l.Get clean upper cover glass 7. with lower cover glass 9. each a slice of cover glass, make the center superposition of two panels, length limit respectively parallel, 8. sample chamber substrate be pressed on centre and clamp, making sample chamber B, as shown in B in Fig. 1.
Use syringe that 502 quick-drying gelatins are dropped to slide edge, due to capillary effect, glue is full of the space between two slides very soon, glue dries after about tens seconds.After treating that both sides slide glues, shape just becomes the sample cavity of a long strip type therebetween, the opening that two ends are respectively stayed, for sample introduction and exhaust.
Before using, first sample solution is added and inject sample chamber B, and seal two-port respectively with Hmp grease (Mobil Duracell), then with transparent adhesive tape, sample chamber B is fixed on the below of temperature control A, the upper slide noting B 7. upward, is now gone up slide and 7. will be just in time in the square groove of slide support Ban⑤ center.
Temperature control instrument C: the temperature control gauge outfit that the present embodiment adopts the many safe instrument in Yuyao to produce and corresponding constant voltage dc source are as external control element.This temperature control instrument electromagnetic relay controls the sense of current by TEC, thus control TEC 2. below thermal conductive metal plate temperature variation 3..Temperature control instrument C comprises temperature-controlled power, feedback controller and wire etc. and belongs to existing market outsourcing device.
Micro thermocouple (U.S. Omega) is placed in sample chamber B, the temperature of solution in sample chamber can be detected in real time.Experiment finds that the present embodiment effectively can regulate temperature in sample chamber, and effective range of adjustment is 6-90 DEG C, can the basic constant temperature of holding temperature within the scope of this, and temperature fluctuation range is ± 0.5 DEG C.
The utility model can the minisize sample cell of real-time implementation temperature adjusting, by controlling the temperature with the heat-conducting metal of sample close contact, carrys out the temperature of sample in indirect Quality control room.The temperature of heat-conducting metal is measured in real time by thermocouple thermometer.This sample chamber can meet in microscopic biological, physics, chemical experiment to regulate and control experimental temperature and the requirement of constant temperature.
The utility model can the minisize sample cell of real-time implementation temperature adjusting, has technical indicator as shown in table 1.
Table 1
Project name Parameter
Temperature control scope 6-90℃
Temperature-controlled precision ±0.5℃
Apparent size Be less than 90mm × 40mm × 14mm (not comprising external cord)
The temperature-responsive time ≤3min

Claims (3)

1.一种实时温控微型样品室,其特征在于:包括控温装置A、样品室B和控温仪表C三部分,其中:  1. A real-time temperature-controlled miniature sample chamber, characterized in that: it comprises three parts of temperature control device A, sample chamber B and temperature control instrument C, wherein: 控温装置A包括U型支撑板(①),半导体制冷片TEC)②),TEC的金属导热片(③),热电偶探头(④),玻片支撑板(⑤)和隔热板(⑥);  The temperature control device A includes a U-shaped support plate (①), a semiconductor cooling chip (TEC)②), a metal heat conduction sheet of the TEC (③), a thermocouple probe (④), a slide support plate (⑤) and a heat shield (⑥ ); 控温装置A的U型支撑板(①)两侧下方为两个半导体制冷片TEC(②),每个半导体制冷片TEC(②)下方均装有TEC的金属导热片(③),两个TEC的金属导热片(③)下面共同连接玻片支撑板(⑤),玻片支撑板(⑤)下面为两个隔热板(⑥),TEC的金属导热片(③)的方槽开口向下,两个热电偶探头(④)分别安装在两个TEC的金属导热片(③)的方槽内,且使得热电偶探头(④)位于TEC的金属导热片(③)和玻片支撑板(⑤)之间;  Under the U-shaped support plate (①) of the temperature control device A are two semiconductor cooling chips TEC (②), and each semiconductor cooling chip TEC (②) is equipped with a metal heat conducting sheet of the TEC (③). The bottom of the metal heat conduction sheet (③) of the TEC is connected to the slide support plate (⑤), and there are two heat shields (⑥) under the slide support plate (⑤). The square groove of the TEC metal heat conduction sheet (③) opens to Below, the two thermocouple probes (④) are respectively installed in the square grooves of the metal heat conducting sheets (③) of the two TECs, and the thermocouple probes (④) are located between the metal heat conducting sheets (③) of the TEC and the slide support plate (⑤) between; U型支撑板(①),半导体制冷片TEC(②),TEC的金属导热片(③),热电偶探头(④),玻片支撑板(⑤)使用导热胶粘合,然后将隔热板(⑥)使用普通粘合剂粘合在玻片支撑板(⑤)的下方,最终组成控温装置A;  U-shaped support plate (①), semiconductor cooling chip TEC (②), metal heat conduction sheet of TEC (③), thermocouple probe (④), glass slide support plate (⑤) are bonded with thermally conductive adhesive, and then the heat insulation plate (⑥) Adhere to the bottom of the glass slide support plate (⑤) with ordinary adhesive, and finally form the temperature control device A; 样品室B包括上盖玻片(⑦),样品室基板(⑧)和下盖玻片(⑨),样品室基板(⑧)为带有方孔的金属片;  Sample chamber B includes an upper cover glass (⑦), a sample chamber substrate (⑧) and a lower cover glass (⑨), and the sample chamber substrate (⑧) is a metal sheet with a square hole; 将上盖玻片(⑦)、样品室基板(⑧)和下盖玻片(⑨)用502速干胶顺次粘合,从而成样品室B,由于样品室基板(⑧)为带有方孔的金属片,因此粘合后会在上盖玻片(⑦)和下盖玻片(⑨)中间形成一个长条形的腔体,并在两侧分别留有一个小孔,可用于加样和排气;  Glue the upper cover glass (⑦), the sample chamber substrate (⑧) and the lower cover glass (⑨) sequentially with 502 quick-drying glue to form the sample chamber B. Since the sample chamber substrate (⑧) has a square hole, so after bonding, a long strip-shaped cavity will be formed between the upper cover glass (⑦) and the lower cover glass (⑨), and a small hole is left on both sides, which can be used for adding sample and exhaust; 控温仪表C包括控温电源、反馈控制器以及导线;  Temperature control instrument C includes temperature control power supply, feedback controller and wires; 控温装置A和样品室B使用透明胶连接,半导体制冷片TEC(②)的导线和热电偶探头(④)的导线需连接至控温仪表C的反馈控制器上。  The temperature control device A and the sample chamber B are connected with transparent glue, and the wires of the semiconductor refrigeration chip TEC (②) and the wires of the thermocouple probe (④) need to be connected to the feedback controller of the temperature control instrument C. the 2.根据权利要求1所述的一种实时温控微型样品室,其特征在于:可以通过改变样品室基板(⑧)的厚度来改变样品室容积。  2. A real-time temperature-controlled miniature sample chamber according to claim 1, characterized in that the volume of the sample chamber can be changed by changing the thickness of the sample chamber substrate (⑧). the 3.根据权利要求1所述的一种实时温控微型样品室,其特征在于:控温装置A中的各个部件之间使用导热胶连接,保证热传导能力与机械强度,并且保证U型支撑板(①)与TEC的金属导热片(③)不直接接触,降低半导体制冷片TEC(②)的冷热面间的热传导。  3. A real-time temperature-controlled miniature sample chamber according to claim 1, characterized in that: the various components in the temperature control device A are connected with thermally conductive glue to ensure thermal conductivity and mechanical strength, and to ensure that the U-shaped support plate (①) It is not in direct contact with the metal heat conducting sheet (③) of the TEC, which reduces the heat conduction between the cold and hot surfaces of the semiconductor cooling sheet TEC (②). the
CN201420591122.8U 2014-10-13 2014-10-13 Real-time temperature control minisize sample cell Expired - Fee Related CN204143278U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104267766A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Real-time temperature-control micro sample room
JP2019510243A (en) * 2016-03-21 2019-04-11 シァメンタレントバイオメディカルテクノロジー カンパニーリミテッドXiamen Talent Biomedical Technology Company, Ltd. Heat treatment device for sample slide detection
CN109682365A (en) * 2018-12-21 2019-04-26 上海航天控制技术研究所 A kind of thermostat for oscillation gyro combination
CN111521556A (en) * 2020-05-14 2020-08-11 西北农林科技大学 Sample room, temperature-adjusting carrying device, microscope temperature-controlling observation platform and system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104267766A (en) * 2014-10-13 2015-01-07 中国科学技术大学 Real-time temperature-control micro sample room
JP2019510243A (en) * 2016-03-21 2019-04-11 シァメンタレントバイオメディカルテクノロジー カンパニーリミテッドXiamen Talent Biomedical Technology Company, Ltd. Heat treatment device for sample slide detection
CN109682365A (en) * 2018-12-21 2019-04-26 上海航天控制技术研究所 A kind of thermostat for oscillation gyro combination
CN111521556A (en) * 2020-05-14 2020-08-11 西北农林科技大学 Sample room, temperature-adjusting carrying device, microscope temperature-controlling observation platform and system

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Granted publication date: 20150204

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