CN204143278U - Real-time temperature control minisize sample cell - Google Patents
Real-time temperature control minisize sample cell Download PDFInfo
- Publication number
- CN204143278U CN204143278U CN201420591122.8U CN201420591122U CN204143278U CN 204143278 U CN204143278 U CN 204143278U CN 201420591122 U CN201420591122 U CN 201420591122U CN 204143278 U CN204143278 U CN 204143278U
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- sample chamber
- temperature control
- tec
- support plate
- temperature
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- 239000000523 sample Substances 0.000 claims abstract description 86
- 239000002184 metal Substances 0.000 claims abstract description 31
- 229910052751 metal Inorganic materials 0.000 claims abstract description 31
- 239000006059 cover glass Substances 0.000 claims abstract description 22
- 239000000758 substrate Substances 0.000 claims abstract description 18
- 239000004065 semiconductor Substances 0.000 claims abstract description 14
- 238000001816 cooling Methods 0.000 claims abstract 6
- 239000011521 glass Substances 0.000 claims abstract 3
- 239000003292 glue Substances 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 6
- 108010010803 Gelatin Proteins 0.000 description 5
- 229920000159 gelatin Polymers 0.000 description 5
- 235000019322 gelatine Nutrition 0.000 description 5
- 235000011852 gelatine desserts Nutrition 0.000 description 5
- 239000002390 adhesive tape Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241001206171 Stomoxys omega Species 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
本实用新型提出一种实时温控微型样品室，包括控温装置(A)、样品室(B)和控温仪表(C)三部分。控温装置(A)包括U型支撑板(①)、半导体制冷片(TEC)(②)、TEC的金属导热片(③)、热电偶探头(④)、玻片支撑板(⑤)和隔热板(⑥)。样品室(B)包括上盖玻片(⑦)、样品室基板(⑧)和下盖玻片(⑨)。控温仪表(C)包括控温电源、反馈控制器和导线。本实用新型可对样品的温度进行实时控制和调节，温控范围为6-90℃，温度响应时间≤3min，温度控制精度为±0.5℃。样品室不会引入任何机械振动，无噪声源；容积可调，最少仅需10μl溶液即可充满样品室；体积小，特别满足由于高倍率物镜导致的显微镜载物台空间十分有限的情况。
The utility model proposes a real-time temperature-controlled miniature sample room, which includes three parts: a temperature control device (A), a sample room (B) and a temperature control instrument (C). The temperature control device (A) includes a U-shaped support plate (①), a semiconductor cooling chip (TEC) (②), a metal heat conduction sheet of the TEC (③), a thermocouple probe (④), a glass slide support plate (⑤) and a spacer. Hot plate (⑥). The sample chamber (B) includes an upper cover glass (⑦), a sample chamber substrate (⑧) and a lower cover glass (⑨). The temperature control instrument (C) includes a temperature control power supply, a feedback controller and wires. The utility model can control and adjust the temperature of the sample in real time, the temperature control range is 6-90°C, the temperature response time is ≤3min, and the temperature control accuracy is ±0.5°C. The sample chamber does not introduce any mechanical vibration and has no noise source; the volume is adjustable, and only 10 μl of solution is needed to fill the sample chamber; the small size is especially suitable for the situation where the space of the microscope stage is very limited due to the high magnification objective lens.
Description
Technical field
The utility model belongs to micro-container technical field, be specifically related to a kind of can the minisize sample cell of real-time implementation temperature adjusting.
Background technology
In microexamination experiment and micromanipulation experiment, common sample chamber, owing to not having temp regulating function, can only at room temperature be tested.But in actual applications, a lot of biological, physics and chemical experiment need to maintain specified temp (not necessarily room temperature) in experimentation, or carrying out and change temperature with experiment, therefore need to install the sample chamber with temperature control device additional.
The general volume of current existing sample chamber attemperating unit is comparatively large, and multi-band has the noise sources such as fan, cannot meet and use the narrow working space of high magnification microcobjective and the ultra-low noise requirement needed for high-acruracy survey.
Utility model content
The purpose of this utility model is: provide a kind of minisize sample cell that can realize real time temperature regulation and control, in experimentation, the temperature of sample indoor liquid controlled in real time and regulate, sample chamber volume is little, do not introduce any mechanical vibration, have preferably temperature-time responding ability and temperature degree of regulation.
The technical solution adopted in the utility model is: a kind of real-time temperature control minisize sample cell, comprises temperature regulating device A, sample chamber B and temperature control instrument C, wherein:
1. temperature regulating device A comprises U-shaped back up pad, semiconductor chilling plate (TEC) 2., the metal conducting strip of TEC 3., thermocouple probe 4., slide support plate 5. with thermal insulation board 6.;
The U-shaped back up pad of temperature regulating device A 1. down either side be two semiconductor chilling plates (TEC) 2., 3. each semiconductor chilling plate (TEC) 2. below is all equipped with the metal conducting strip of TEC, 5. the metal conducting strip of two TEC is 3. common below connects slide support plate, slide support plate be 5. below two thermal insulation boards 6., Open Side Down for the square groove of the metal conducting strip (3.) of TEC, in the metal conducting strip that 4. two thermocouple probes are arranged on two TEC respectively square groove 3., and make thermocouple probe 4. TEC metal conducting strip 3. and slide support plate 5. between.
1., 2., the metal conducting strip of TEC 3. for semiconductor chilling plate (TEC) for U-shaped back up pad, thermocouple probe 4., 5. slide support plate uses heat-conducting glue to bond, and is then 6. used by thermal insulation board common adhesive to be bonded in slide support plate below 5., final composition temperature regulating device A.
7. sample chamber B comprises cover glass, sample chamber substrate 8. with lower cover glass 9., 8. sample chamber substrate is the sheet metal with square hole.
By upper cover glass 7., 9. 8. sample chamber substrate bond with 502 quick-drying gelatins in turn with lower cover glass, from forming sample chamber B, due to the sheet metal that 8. sample chamber substrate is with square hole, therefore understand after bonding and 7. 9. form the cavity of a strip in centre with lower cover glass at upper cover glass, and an aperture is left respectively in both sides, can be used for application of sample and exhaust.
Temperature control instrument C comprises temperature-controlled power, feedback controller and wire.
Temperature regulating device A and sample chamber B use transparent adhesive tape to be connected, and semiconductor chilling plate (TEC) wire 2. and thermocouple probe wire 4. need be connected on the feedback controller of temperature control instrument C.
Further, sample chamber volume can be changed by changing sample chamber substrate thickness 8..
Further, connect with heat-conducting glue between all parts in temperature regulating device A, ensure thermal conduction capability and physical strength, and ensure that 3. 1. U-shaped back up pad directly do not contact with the metal conducting strip of TEC, reduce the heat transfer between semiconductor chilling plate (TEC) cold and hot surface 2..
The utility model has the advantage of:
1. the utility model controls in real time the temperature in sample chamber and regulates in experimentation, has preferably temperature-time responding ability and temperature degree of regulation.
2. the utility model sample chamber volume is little, and practicality is wide, meets the situation that the microscope stage space that causes due to high magnification object lens is very limited especially.
3. the utility model does not introduce any mechanical vibration, noiseless source, can be used for high-precision measurement.
4. the internal adjustable volume of sample chamber (B) in the utility model, the minimum 10 μ l solution that only need can be full of sample chamber when 8. thickness is 0.1mm (sample chamber substrate).
Accompanying drawing explanation
Fig. 1 is that the utility model is a kind of can the minisize sample cell structural representation of real-time implementation temperature adjusting, and in figure, numbering is followed successively by:
1. be U-shaped back up pad,
2. be TEC (semiconductor chilling plate),
3. be the metal conducting strip of TEC,
4. be thermocouple probe,
5. be slide support plate,
6. be thermal insulation board,
7. be upper cover glass,
8. be sample chamber substrate,
9. be lower cover glass;
Fig. 2 is can the minisize sample cell finished product schematic diagram of real-time implementation temperature adjusting;
Fig. 3 be U-shaped back up pad 1., wherein scheme (a) be U-shaped back up pad vertical view, figure (b) be U-shaped back up pad side view; Unit: mm;
Fig. 4 be the metal conducting strip of TEC 3., wherein to scheme (a) be TEC metal conducting strip vertical view, the metal conducting strip side view that figure (b) is TEC; Unit: mm;
Fig. 5 be slide support plate 5., 75mm × 25mm × 1mm;
Fig. 6 be sample chamber substrate 8., 32mm × 22mm × 0.1mm (0.1mm is substrate thickness, and its value is optional).
Embodiment
The utility model is further illustrated below in conjunction with accompanying drawing and specific embodiment.
The utility model proposes a kind of can the minisize sample cell of real-time implementation temperature adjusting.The structure of this minisize sample cell is made up of temperature regulating device A, sample chamber B and temperature control instrument C tri-part.As shown in Figure 1, the real-time temperature control minisize sample cell be combined to form is the pattern of Fig. 2 to the structure of temperature regulating device A and sample chamber B.
Temperature regulating device A: by element 1.-5. use heat-conducting glue be bonded, 6. use 502 quick-drying gelatins to stick to 5., as shown in Figure 2.U-shaped back up pad is 1.: external dimensions 90mm × 40mm × 3mm, side symcenter is processed into the square groove of 50mm × 30mm.The one side use heat-conducting glue that U-shaped portion is divided and TEC 2. (20mm × 20mm × 3mm, model tes1-04902) link.The selection of TEC top and bottom 2. will coordinate with the both positive and negative polarity of ambient controller, connects and instead can cannot realize temperature adjusting.3. TEC one side is 2. connected with the metal conducting strip of TEC by heat-conducting glue.The metal conducting strip of TEC is 3.: external dimensions 30mm × 25mm × 5mm, has 3mm × 3mm square groove, as shown in Figure 4.In the metal conducting strip that 4. thermocouple probe is fixed on TEC by heat-conducting glue groove 3..Finally use heat-conducting glue or 502 quick-drying gelatins respectively by slide support plate 5. and thermal insulation board be 6. fixed on the metal conducting strip 3. below of TEC successively.Slide support plate 5. external dimensions 75mm × 25mm, Central Symmetry is processed into 22mm × 19mm square groove, and thickness is 1mm, as shown in Figure 5.
Sample chamber B: by element 7.-9. use 502 quick-drying gelatins to be bonded.Cover glass 7. (sailing boat board) apparent size is 18mm × 18mm × 0.17mm; Lower cover glass size 9. (Corning Cover Glass Cat.No.2865-22) is 22mm × 22mm × 0.17mm; Sample chamber substrate is 8.: scale copper, outside dimension 32mm × 22mm × 0.1mm, 22mm × 4mm square hole is processed at center, as shown in Figure 6.Sample chamber substrate thickness is optional, if use 0.1mm thin slice, then sample chamber internal volume about 10 μ l.Get clean upper cover glass 7. with lower cover glass 9. each a slice of cover glass, make the center superposition of two panels, length limit respectively parallel, 8. sample chamber substrate be pressed on centre and clamp, making sample chamber B, as shown in B in Fig. 1.
Use syringe that 502 quick-drying gelatins are dropped to slide edge, due to capillary effect, glue is full of the space between two slides very soon, glue dries after about tens seconds.After treating that both sides slide glues, shape just becomes the sample cavity of a long strip type therebetween, the opening that two ends are respectively stayed, for sample introduction and exhaust.
Before using, first sample solution is added and inject sample chamber B, and seal two-port respectively with Hmp grease (Mobil Duracell), then with transparent adhesive tape, sample chamber B is fixed on the below of temperature control A, the upper slide noting B 7. upward, is now gone up slide and 7. will be just in time in the square groove of slide support Ban⑤ center.
Temperature control instrument C: the temperature control gauge outfit that the present embodiment adopts the many safe instrument in Yuyao to produce and corresponding constant voltage dc source are as external control element.This temperature control instrument electromagnetic relay controls the sense of current by TEC, thus control TEC 2. below thermal conductive metal plate temperature variation 3..Temperature control instrument C comprises temperature-controlled power, feedback controller and wire etc. and belongs to existing market outsourcing device.
Micro thermocouple (U.S. Omega) is placed in sample chamber B, the temperature of solution in sample chamber can be detected in real time.Experiment finds that the present embodiment effectively can regulate temperature in sample chamber, and effective range of adjustment is 6-90 DEG C, can the basic constant temperature of holding temperature within the scope of this, and temperature fluctuation range is ± 0.5 DEG C.
The utility model can the minisize sample cell of real-time implementation temperature adjusting, by controlling the temperature with the heat-conducting metal of sample close contact, carrys out the temperature of sample in indirect Quality control room.The temperature of heat-conducting metal is measured in real time by thermocouple thermometer.This sample chamber can meet in microscopic biological, physics, chemical experiment to regulate and control experimental temperature and the requirement of constant temperature.
The utility model can the minisize sample cell of real-time implementation temperature adjusting, has technical indicator as shown in table 1.
Table 1
| Project name | Parameter |
| Temperature control scope | 6-90℃ |
| Temperature-controlled precision | ±0.5℃ |
| Apparent size | Be less than 90mm × 40mm × 14mm (not comprising external cord) |
| The temperature-responsive time | ≤3min |
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591122.8U CN204143278U (en) | 2014-10-13 | 2014-10-13 | Real-time temperature control minisize sample cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591122.8U CN204143278U (en) | 2014-10-13 | 2014-10-13 | Real-time temperature control minisize sample cell |
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| Publication Number | Publication Date |
|---|---|
| CN204143278U true CN204143278U (en) | 2015-02-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420591122.8U Expired - Fee Related CN204143278U (en) | 2014-10-13 | 2014-10-13 | Real-time temperature control minisize sample cell |
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| Country | Link |
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| CN (1) | CN204143278U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| JP2019510243A (en) * | 2016-03-21 | 2019-04-11 | シァメンタレントバイオメディカルテクノロジー カンパニーリミテッドＸｉａｍｅｎ Ｔａｌｅｎｔ Ｂｉｏｍｅｄｉｃａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｍｐａｎｙ， Ｌｔｄ． | Heat treatment device for sample slide detection |
| CN109682365A (en) * | 2018-12-21 | 2019-04-26 | 上海航天控制技术研究所 | A kind of thermostat for oscillation gyro combination |
| CN111521556A (en) * | 2020-05-14 | 2020-08-11 | 西北农林科技大学 | Sample room, temperature-adjusting carrying device, microscope temperature-controlling observation platform and system |
-
2014
- 2014-10-13 CN CN201420591122.8U patent/CN204143278U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| JP2019510243A (en) * | 2016-03-21 | 2019-04-11 | シァメンタレントバイオメディカルテクノロジー カンパニーリミテッドＸｉａｍｅｎ Ｔａｌｅｎｔ Ｂｉｏｍｅｄｉｃａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｍｐａｎｙ， Ｌｔｄ． | Heat treatment device for sample slide detection |
| CN109682365A (en) * | 2018-12-21 | 2019-04-26 | 上海航天控制技术研究所 | A kind of thermostat for oscillation gyro combination |
| CN111521556A (en) * | 2020-05-14 | 2020-08-11 | 西北农林科技大学 | Sample room, temperature-adjusting carrying device, microscope temperature-controlling observation platform and system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150204 Termination date: 20201013 |