CN204111768U - A kind of hypobaric hypoxia cell culture apparatus - Google Patents

A kind of hypobaric hypoxia cell culture apparatus Download PDF

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Publication number
CN204111768U
CN204111768U CN201420550339.4U CN201420550339U CN204111768U CN 204111768 U CN204111768 U CN 204111768U CN 201420550339 U CN201420550339 U CN 201420550339U CN 204111768 U CN204111768 U CN 204111768U
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China
Prior art keywords
inlet mouth
utility
hypobaric hypoxia
model
cell culture
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Expired - Fee Related
Application number
CN201420550339.4U
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Chinese (zh)
Inventor
潘耀柱
马慧萍
王璇
白海
王存邦
尹姣姣
韩燕霞
李文博
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LANZHOU GEN HOSPITAL LANZHOU MILITARY AREA COMMAND PLA
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LANZHOU GEN HOSPITAL LANZHOU MILITARY AREA COMMAND PLA
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Priority to CN201420550339.4U priority Critical patent/CN204111768U/en
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model provides a kind of hypobaric hypoxia cell culture apparatus, comprise hypoxemia and cultivate air chamber, vacuum extractor, tensimeter and oxygen concn table, wherein, described hypoxemia is cultivated air chamber and is comprised inlet mouth and air outlet, equal mounted valve on described inlet mouth and air outlet; Described inlet mouth is connected with tensimeter, oxygen concn table and vacuum extractor successively by pipeline.The utility model is easy and simple to handle, and energy simulated high altitude hypobaric hypoxia environment cell cultures, can't harm people, and effectively save research expenditure.

Description

A kind of hypobaric hypoxia cell culture apparatus
Technical field
The utility model belongs to bio-medical instrument field, particularly relates to a kind of hypobaric hypoxia cell culture apparatus.
Background technology
How to realize simulated high altitude hypobaric hypoxia environment cell cultures, international and domesticly at present there is no report.Prior art hypoxemia cultivates air chamber only can carry out the cultivation such as hypoxemia, hyperoxia; If carry out hypobaric hypoxia cell cultures, only at the built-in cell culture incubator of low-pressure oxygen cabin, but there is a lot of shortcoming: 1, safeguard about more than 4000 yuan of low-pressure oxygen cabin expense every day; 2, every day, researchist passed in and out the inconveniences such as low-pressure oxygen cabin, and hypobaric hypoxia condition also can cause damage to researchist's health.
Utility model content
The purpose of this utility model is to provide a kind of hypobaric hypoxia cell culture apparatus, is intended to solve the deficiency in above-mentioned background technology.
The utility model realizes like this, a kind of hypobaric hypoxia cell culture apparatus, comprises hypoxemia and cultivates air chamber, vacuum extractor, tensimeter and oxygen concn table, wherein, described hypoxemia is cultivated air chamber and is comprised inlet mouth and air outlet, equal mounted valve on described inlet mouth and air outlet; Described inlet mouth is connected with tensimeter, oxygen concn table and vacuum extractor successively by pipeline.
Compared to the shortcoming and defect of prior art, the utility model has following beneficial effect: the utility model is easy and simple to handle, and energy simulated high altitude hypobaric hypoxia environment cell cultures, can't harm people, and effectively save research expenditure.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model hypobaric hypoxia cell culture apparatus.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
As shown in Figure 1, Fig. 1 is the structural representation of the utility model hypobaric hypoxia cell culture apparatus.
A kind of hypobaric hypoxia cell culture apparatus, comprises hypoxemia and cultivates air chamber 1, vacuum extractor 2, tensimeter 3 and oxygen concn table 4, and wherein, described hypoxemia is cultivated air chamber and comprised inlet mouth 5 and air outlet 6, equal mounted valve on described inlet mouth 5 and air outlet 6; Described inlet mouth 5 is connected with tensimeter 3, oxygen concn table 4 and vacuum extractor 2 successively by pipeline.
In actual application of the present utility model, comprise the following steps:
(1) open the valve of inlet mouth 5 and air outlet 6, premixed gas (as 2% oxygen, 5% carbonic acid gas, 93% nitrogen) is filled with hypoxemia and cultivates in air chamber 13 ~ 7 minutes.
Under the equal opening status of air inlet/outlet, by inlet mouth preliminary filling mixed gas (as 2% oxygen, 5% carbonic acid gas, 93% nitrogen), preliminary filling, after 3 ~ 7 minutes, is cultivated in air chamber and is reached preliminary filling mixed gas concentration state.
(2) folder closes the valve that hypoxemia cultivates air chamber 1 inlet mouth 5, and air outlet 6 connects vacuum extractor 2 simultaneously.
(3) carry out vacuum suction by preset pressure by vacuum extractor 2, and regulate oxygen concn 4 by precharge of gas, reach desirable hypobaric hypoxia state.
(4) after reaching predetermined pressure, folder closes the valve of air outlet 6, hypoxemia is cultivated air chamber 1 and is placed in cell culture incubator cultivation.
The utility model is easy and simple to handle, and energy simulated high altitude hypobaric hypoxia environment cell cultures, can't harm people, and effectively save research expenditure.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.

Claims (1)

1. a hypobaric hypoxia cell culture apparatus, is characterized in that, comprises hypoxemia and cultivates air chamber, vacuum extractor, tensimeter and oxygen concn table, and wherein, described hypoxemia is cultivated air chamber and comprised inlet mouth and air outlet, equal mounted valve on described inlet mouth and air outlet; Described inlet mouth is connected with tensimeter, oxygen concn table and vacuum extractor successively by pipeline.
CN201420550339.4U 2014-09-22 2014-09-22 A kind of hypobaric hypoxia cell culture apparatus Expired - Fee Related CN204111768U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420550339.4U CN204111768U (en) 2014-09-22 2014-09-22 A kind of hypobaric hypoxia cell culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420550339.4U CN204111768U (en) 2014-09-22 2014-09-22 A kind of hypobaric hypoxia cell culture apparatus

Publications (1)

Publication Number Publication Date
CN204111768U true CN204111768U (en) 2015-01-21

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Family Applications (1)

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CN (1) CN204111768U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862218A (en) * 2015-05-28 2015-08-26 首都医科大学附属北京世纪坛医院 Low-pressure and low-oxygen cell experiment device
CN109055221A (en) * 2018-10-16 2018-12-21 兰州军区兰州总医院 A kind of pressure control cell hypoxia incubator
CN118006464A (en) * 2024-04-10 2024-05-10 江苏立维斯德生物技术有限公司 Portable cell hypoxia culture device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862218A (en) * 2015-05-28 2015-08-26 首都医科大学附属北京世纪坛医院 Low-pressure and low-oxygen cell experiment device
CN109055221A (en) * 2018-10-16 2018-12-21 兰州军区兰州总医院 A kind of pressure control cell hypoxia incubator
CN118006464A (en) * 2024-04-10 2024-05-10 江苏立维斯德生物技术有限公司 Portable cell hypoxia culture device

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150121

Termination date: 20150922

EXPY Termination of patent right or utility model