CN204085982U - A kind of based on microscopical cell point device for mark and microscope - Google Patents
A kind of based on microscopical cell point device for mark and microscope Download PDFInfo
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- CN204085982U CN204085982U CN201420556286.7U CN201420556286U CN204085982U CN 204085982 U CN204085982 U CN 204085982U CN 201420556286 U CN201420556286 U CN 201420556286U CN 204085982 U CN204085982 U CN 204085982U
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Abstract
The utility model relates to a kind of based on microscopical cell point device for mark and microscope, and this cell point device for mark comprises: fixed part, for being fixed on microscope; Connecting portion, is connected with described fixed part; And some mark portion, be connected with described connecting portion, the ending vertical ground in described some mark portion points to described microscopical light path center, for marking the target location on objective table.Described microscope comprises this cell point device for mark.The cell point device for mark that the present embodiment provides, can be fixed on microscope, as long as by a center for mark portion aligming microscope light path, just can be made marks to target cell by lower pressure point mark portion, accuracy is high, saves manpower; And by offering regulating tank on fixed part, the convenient adjustment position of cell point device for mark on microscope.
Description
Technical field
The utility model relates to microscopy field, particularly relates to a kind of based on microscopical cell point device for mark and the microscope comprising this cell point device for mark.
Background technology
Transmission electron microscope (Transmission electron microscope, TEM) technology detects and study the important means of virus, is one of one preferred technique of the unknown cause of disease of diagnosis and identification of organism attack of terrorism cause of disease.Ultramicrotomy is the important technology forming biological TEM technical system.Under normal circumstances, adhere-wall culture cell ultra-thin section sample making course needs following steps to complete: first directly scrape de-cell with cell sleaker or make cell detachment cultivate interface by trypsinization, again the cell of de-wall is carried out centrifugally making it be gathered into agglomerate, then aldehydes and two fixing, the graded ethanol of osmium tetroxide (or epoxypropane or acetone) dehydration is carried out to cell mass, resin infiltrates, embeds, is polymerized, repaiies the handling procedure such as block and section.The process such as scraping and enzymic digestion can cause cell ultrastructure to change, thus is difficult to the true nature state reflecting attached cell.Above-mentioned process makes cell detachment fixed position, agglomerate is formed through centrifugal gathering, cell is made to be randomly dispersed in cell mass, after above-mentioned process, section there is a lot of cell, be difficult to realize by the viewed same cell of transmission electron microscope observation optical microscope, greatly reduce the detection efficiency of transmission electron microscope to cell ultra-thin section.
In order to observe the viewed target cell of optical microscope under transmission electron microscope, applicant establishes locating super-thin microtomy (application number is the patent " a kind of locating super-thin dicing method for infecting viral sick cell " of 201310470347.8) previous patent.When applying locating super-thin microtomy, the conveniently position of target-marking cell before section, usually mark target cell position in the mode of craft, concrete grammar is the position marking target cell in the microscopic examination visual field with marking pen on microslide.Because the micron-sized cell position of manual markings is larger for manual operations difficulty, thus higher requirement is had to operator, such as require that operator has good arm steady qualitative, can not occur that in labeling process arm is shaken, and the good stereoscopic sensation etc. under needing operator to possess microscope.Once a little shake occurs hand, the position of mark position wide cell will be caused.In addition, the nib for the marking pen marked is general comparatively thick, and the size in labeling dye region is much larger than target cell region, and this also causes mark position inaccurate.As mentioned above, manual mode accuracy that cell position is marked and efficiency all lower.
Utility model content
Technical matters
In view of this, the technical problems to be solved in the utility model is, how the marking the position of the target cell on microslide of precise and high efficiency, to facilitate the follow-up positioning searching for target cell, isolated cell and to carry out the ultra-thin section of target cell.
Solution
In order to solve the problems of the technologies described above, according to an embodiment of the present utility model, provide a kind of based on microscopical cell point device for mark, comprising:
Fixed part, for being fixed on microscope;
Connecting portion, is connected with described fixed part; And
Point mark portion, is connected with described connecting portion, and the ending vertical ground in described some mark portion points to described microscopical light path center, for marking the target location on objective table.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described fixed part offers at least one pair of regulating tank, described regulating tank is positioned on horizontal direction and/or vertical direction.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described some mark portion comprises:
Casting die, is arranged on one end away from described fixed part of described connecting portion by elastomeric element; And
Point tender, to be arranged on described casting die perpendicular to the direction of described objective table, is hollow structure.
In order to solve the problems of the technologies described above, according to another embodiment of the present utility model, additionally provide a kind of microscope, comprise above-mentioned based on microscopical cell point device for mark.
In order to solve the problems of the technologies described above, according to another embodiment of the present utility model, additionally provide a kind of based on microscopical cell point device for mark, comprising:
Fixed part, for being fixed on microscope;
Adjusting portion, is connected with described fixed part, and the length of described adjusting portion and width can regulate; And
Point mark portion, is connected with described fixed part, and the ending vertical ground in described some mark portion points to described microscopical light path center, for marking the target location on objective table.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described fixed part comprises:
Fixed head, described fixed head offers at least one pair of regulating tank, and described regulating tank is positioned on horizontal direction and/or vertical direction; And
Web member, is connected in the horizontal direction rotationally with described adjusting portion.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, the part that described web member is connected with described adjusting portion carries out locking by pin.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described adjusting portion comprises:
First regulating part, described first regulating part is provided with slide rail on the length direction along described adjusting portion; And
Second regulating part, described second regulating part is provided with slide block on the length direction along described adjusting portion, and described slide block slides in described slide rail, and described slide block is provided with securing member.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described slide block is arranged on slider carrier, and described slider carrier is connected with described second regulating part by screw.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, the end of described slide rail is provided with block piece and locating part.
For above-mentioned based on microscopical cell point device for mark, in a kind of possible implementation, described some mark portion comprises:
Casting die, is arranged on one end away from described fixed part of described adjusting portion by elastomeric element; And
Point tender, to be arranged on described casting die perpendicular to the direction of described objective table, is hollow structure.
In order to solve the problems of the technologies described above, according to an embodiment more of the present utility model, provide a kind of microscope, comprise above-mentioned based on microscopical cell point device for mark.
Beneficial effect
The present embodiment provide based on microscopical cell point device for mark, can be fixed on microscope, as long as by a center for mark portion aligming microscope light path, just can be made marks to target cell by lower pressure point mark portion, accuracy is high, save manpower.And by offering regulating tank on fixed part, the convenient adjustment position of cell point device for mark on microscope.Further, because the length of adjusting portion and width can both regulate, thus this cell point device for mark can be applied on the microscope of different size.The cell point device for mark that the utility model provides, can replace manually easily realizing precise marking to the position of micron order cell, to operator without particular/special requirement, simple to operate, degree of accuracy is high, reproducible, is easy to promote.
According to below with reference to the accompanying drawings to detailed description of illustrative embodiments, further feature of the present utility model and aspect will become clear.
Accompanying drawing explanation
Comprise in the description and form the accompanying drawing of a part for instructions and instructions together illustrates exemplary embodiment of the present utility model, characteristic sum aspect, and for explaining principle of the present utility model.
Fig. 1 illustrates the structural representation based on microscopical cell point device for mark provided according to the utility model embodiment;
Fig. 2 illustrates the structural representation based on microscopical cell point device for mark provided according to another embodiment of the utility model;
Fig. 3 illustrates the front view based on microscopical cell point device for mark provided according to another embodiment of the utility model;
Fig. 4 illustrates the vertical view based on microscopical cell point device for mark provided according to another embodiment of the utility model;
Fig. 5 illustrates the part-structure schematic diagram based on microscopical cell point device for mark provided according to another embodiment of the utility model;
Fig. 6 illustrates the part-structure schematic diagram based on microscopical cell point device for mark provided according to another embodiment of the utility model;
Fig. 7 illustrates the microscopical structural representation provided according to another embodiment of the utility model;
Fig. 8 illustrates the schematic diagram made a mark on microslide based on microscopical cell point device for mark provided according to the utility model embodiment; And
Fig. 9 (A)-Fig. 9 (D) is respectively the schematic diagram of the target cell marked based on microscopical cell point device for mark adopting the utility model to provide.
Embodiment
Various exemplary embodiment of the present utility model, characteristic sum aspect is described in detail below with reference to accompanying drawing.The same or analogous element of Reference numeral presentation function identical in accompanying drawing.Although the various aspects of embodiment shown in the drawings, unless otherwise indicated, accompanying drawing need not be drawn in proportion.
Word " exemplary " special here means " as example, embodiment or illustrative ".Here need not be interpreted as being better than or being better than other embodiment as any embodiment illustrated by " exemplary ".
In addition, in order to better the utility model is described, in embodiment hereafter, give numerous details.It will be appreciated by those skilled in the art that do not have some detail, the utility model can be implemented equally.In some instances, the method known for those skilled in the art, means, element and circuit are not described in detail, so that highlight purport of the present utility model.
Embodiment 1
Fig. 1 illustrates the structural representation based on microscopical cell point device for mark provided according to the utility model embodiment, and as shown in Figure 1, the cell point device for mark 100 that the present embodiment provides comprises fixed part 1, connecting portion 2 and some mark portion 3.
Wherein, cell point device for mark 100, for whole cell point device for mark 100 is fixed on microscope, due to most of microscopical handel having screw, for the ease of installing, is therefore preferably fixed on handel by fixed part 1.Connecting portion 2 is connected with fixed part 1, and some mark portion 3 is connected with connecting portion 2, and the ending vertical ground in some mark portion 3 points to microscopical light path center, can mark the target location on objective table.
Wherein, fixed part 1 offers at least one pair of regulating tank 4, screw etc. can be used to be fixed on microscope by fixed part 1.Regulating tank 4 can be positioned on horizontal direction and/or vertical direction, to regulate the position of whole cell point device for mark 100 on microscope.The shape design of fixed part 1 is not limited to the form cited by the present embodiment, fixed part 1 can be done different designs according to microscopical difference, is processed into difformity and form.
Connecting portion 2 can be rectangular parallelepiped or cylindrical structure.Connecting portion 2 can be fixedly connected with fixed part 1, as long as ensure the length in connecting portion 2 and some mark portion 3, makes invocation point mark portion 3 end just vertically point to the position at microscopes optical path center.Connecting portion 2 also can be connected with fixed part 1 rotationally, such as hinged, and when connecting portion 2 forwards suitable position to, carries out locking by pin, thus can the position in point of adjustment mark portion 3 in the horizontal direction.
Point mark portion 3 comprises casting die 5 and some tender 6.Wherein casting die 5 is arranged on one end away from fixed part 1 of connecting portion 2 by elastomeric element (not shown), and some tender 6 is perpendicular to the direction of objective table being arranged on casting die 5, and some tender 6 is hollow structure.Point tender 6 removably connects with casting die 5, facilitates the replacing of the some tender 6 of different size (such as length) thus.After smearing pigment at the end of a tender 6, by pressing casting die 5, some tender 6 can mark the position of the target cell on microslide.
The cell point device for mark that the present embodiment provides, utilize the screw that microscope carries, without the need to changing microscopical structure, just can cell point device for mark be fixed on microscope, as long as by a center for mark portion aligming microscope light path, just can be made marks to target cell by lower pressure point mark portion, accuracy is high, saves manpower.And by offering regulating tank on fixed part, the convenient adjustment position of cell point device for mark on microscope.
Embodiment 2
Fig. 2 illustrates the structural representation based on microscopical cell point device for mark provided according to another embodiment of the utility model, Fig. 3 illustrates corresponding front view, Fig. 4 illustrates corresponding vertical view, as shown in figs 2-4, this cell point device for mark 200 mainly comprises fixed part 10, adjusting portion 20 and some mark portion 30.
Wherein, fixed part 10 is for being fixed on microscope by whole cell point device for mark 200.Preferably, in the present embodiment, fixed part 10 is fixed on microscopical handel.Adjusting portion 20 is connected with fixed part 10, and adjusting portion 20 is positioned at above objective table, and the width on adjusting portion 20 length in the x-direction in figure 2 and y direction in fig. 2 all can regulate.Point mark portion 30 is connected with adjusting portion 20, marks for the position of the target cell be vertically opposite on microslide.
Specifically, as shown in Figure 5, fixed part 10 comprises fixed head 11 and web member 12.Fixed head 11 offers regulating tank 13, nut can be used to be fixed on handel by fixed head 10 by regulating tank 13.Wherein, the number of regulating tank 13 is preferably 2, regulating tank 13 can be set to vertically or horizontal direction extension, also can all arrange regulating tank 13 in the two directions.By offering the regulating tank 13 with certain length on fixed head 11, the height of fixed part 10 on handel can be regulated, or position in the horizontal direction, that is can regulate the position of whole cell point device for mark 200 on microscope.Web member 12 is for being connected with adjusting portion 20, and web member 12 is fixedly installed on the side of fixed head 11, can be formed with fixed head 11 split and fit together, and also can be integrally formed with fixed head 11.The shape design of fixed part 1 is not limited to the form cited by the present embodiment, fixed part 1 can be done different designs according to microscopical difference, is processed into difformity and form.
Fixed part 10 can be fixedly connected with adjusting portion 20 and also can be connected rotationally, such as hinged.In the present embodiment, web member 12 can comprise parallel and have recess 122 between two of symmetry grip blocks 121, two grip blocks 121, and on two grip blocks, 121 offer the first hole (not shown) respectively, and two the first holes are mutually corresponding.
As shown in Fig. 2 ~ Fig. 5, adjusting portion 20 comprises the first regulating part 21 and the second regulating part 22.Specifically, one end of first regulating part 21 is protuberance 211, the width of the recess 122 on the width of protuberance 211 and web member 12 adapts, and the position this protuberance 211 corresponding to the first hole offers the second hole, thus the hinged of protuberance 211 and recess 122 can be realized by nut 123, namely can realize being rotatably connected of fixed part 10 and adjusting portion 20.In addition, position corresponding on protuberance 211 and recess 122 is also provided with the 3rd corresponding hole 212, when adjustment cell point device for mark 200 makes after invocation point mark portion 30 aims at the position of target cell on microslide, pin is inserted in the 3rd hole 212, can locking fixed part 10 and adjusting portion 20, make it to rotate again, be convenient to a mark portion 30 and firmly make marks.
Second regulating part 22 is connected with the first regulating part 21 is mobilizable in the longitudinal direction, such as, second regulating part 22 can with the first regulating part 21 relative motion in the longitudinal direction, thus make adjusting portion 20 adjustable length in the longitudinal direction, also namely make cell point device for mark 200 adjustable length in the longitudinal direction.
Specifically, in the present embodiment, the side of the first regulating part 21 has slide rail 23, and the side of the second regulating part 22 is provided with slide block 24, is slided, can adjust the length of adjusting portion 20 by slide block 24 in slide rail 23.Slide block 24 has securing member 25, for carrying out fastening when slide block 24 slides into suitable position to slide block 24.Preferably, slide rail 23 is provided with two, arranges two slide blocks 24 in each slide rail 23.For simplification device, only to arrange a slide rail 23 also passable.In addition, securing member 25 can be pin.
Further, the one or both ends of slide rail 23 have block piece 26, and for stopping that slide block 24 skids off slide rail 23, this block piece 26 can be fixed on the first regulating part 21, also can be integrally formed with the first regulating part 21.Block piece 26 is also provided with locating part 27, to adjust the position that slide block 24 can arrive in slide rail 23.Locating part 27 in the present embodiment is specifically as follows screw 29, is stretched out the length of block piece 26 by set screw 29, can the moving range of adjusting slider 24 in slide rail 23, and the quantity of screw 29 and position all adapt with slide rail 23.
As shown in Figure 6, further, the width distance between the second regulating part 22 and the first regulating part 21 is adjustable.Particularly, slide block 24 is fixed on slider carrier 28, slider carrier 28 and the second regulating part 22 split are arranged and are interconnected by screw 29, the length of the second regulating part 22 is stretched out by set screw 29, the distance between slider carrier 28 and the second regulating part 22 can be adjusted, thus realize the width of adjustment cell point device for mark 200.Be illustrated for 4 screws in Fig. 6, in fact 3 screws also can realize the utility model.
As shown in Figure 6, put mark portion 30 and specifically comprise casting die 31 and some tender 32.Wherein, the inner end portion of the second regulating part 22 is provided with elastomeric element (not shown), and one end of casting die 31 is connected with elastomeric element.Point tender 32 is arranged on casting die 31, and perpendicular to the direction of objective table, be hollow structure, the external diameter of the present embodiment mid point tender 32 can be 1.5mm, matches with the external diameter of drill bit in subsequent technique.By pressing casting die 31 or pressing casting die 31 by arranging button 33, a tender 32 can be driven to move straight down, and the position being placed on the target cell on the microslide of microscopes optical path center in point marks.
The cell point device for mark that the present embodiment provides, utilize the screw that microscope carries, without the need to changing microscopical structure, just can cell point device for mark be fixed on microscope, as long as by a center for mark portion aligming microscope light path, and all parts of cell point device for mark is fixed, just can be made marks to target cell by lower pressure point mark portion, accuracy is high, saves manpower.And by offering regulating tank on fixed part, the position of cell point device for mark on microscope conveniently can be regulated, the length of adjusting portion and width can both regulate in addition, thus this cell point device for mark can be applied on the microscope of different size.It should be noted that, although be illustrated for slide block, hinged mode in the present embodiment, those skilled in the art are it is understood that the physical construction such as gear drive, pivotable may be used to realize the utility model.
Embodiment 3
Present embodiments provide a kind of microscope (not shown), comprise the cell point device for mark 100 in above-described embodiment 1.
Embodiment 4
As shown in Figure 7, present embodiments provide a kind of microscope 300, comprise the cell point device for mark 200 in above-described embodiment 2, this cell point device for mark 200 is arranged on the handel 301 of microscope 300.The present embodiment target cell can be the cell that Carrying Green Fluorescent Protein gene viruses infects, and correspondingly microscope 300 can be specially fluorescent microscope, thus is excited can clearly be observed fluorescent target cell by the ultraviolet of microscope 300.
The process that the cell point device for mark 200 pairs of target cells adopting the utility model to provide mark and observe is specific as follows: be placed in by microslide 302 on the objective table 303 of microscope 300, adjustment point mark portion 32 makes it just point to the position at the light path center of microscope 300, the position of adjustment microslide 302, make target cell be positioned at the central region region of microscope 300, the position then pressing the button 33 pairs of target cells marks.As shown in Figure 8, the position that microslide 302 corresponds to target cell has a circle, is namely the mark that cell point device for mark 200 does.Fig. 9 (A) and Fig. 9 (B) is respectively at microscope 300 times and the target cell seen under visible light, then ultra-thin section and observation are carried out to this target cell, easily can find a large amount of virions contained in cell, wherein Fig. 9 (C) shows the target cell after amplification, and Fig. 9 (D) shows the virus comprised in target cell.
The above; be only embodiment of the present utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; change can be expected easily or replace, all should be encompassed within protection domain of the present utility model.Therefore, protection domain of the present utility model should be as the criterion with the protection domain of described claim.
Claims (12)
1., based on a microscopical cell point device for mark, it is characterized in that, comprising:
Fixed part, for being fixed on microscope;
Connecting portion, is connected with described fixed part; And
Point mark portion, is connected with described connecting portion, and the ending vertical ground in described some mark portion points to described microscopical light path center, for marking the target location on objective table.
2. according to claim 1 based on microscopical cell point device for mark, it is characterized in that, described fixed part offers at least one pair of regulating tank, described regulating tank is positioned on horizontal direction and/or vertical direction.
3. according to claim 1 and 2ly it is characterized in that based on microscopical cell point device for mark, described some mark portion comprises:
Casting die, is arranged on one end away from described fixed part of described connecting portion by elastomeric element; And
Point tender, to be arranged on described casting die perpendicular to the direction of described objective table, is hollow structure.
4. a microscope, is characterized in that, comprise described in any one of claim 1-3 based on microscopical cell point device for mark.
5., based on a microscopical cell point device for mark, it is characterized in that, comprising:
Fixed part, for being fixed on microscope;
Adjusting portion, is connected with described fixed part, and the length of described adjusting portion and width can regulate; And
Point mark portion, is connected with described fixed part, and the ending vertical ground in described some mark portion points to described microscopical light path center, for marking the target location on objective table.
6. according to claim 5 based on microscopical cell point device for mark, it is characterized in that, described fixed part comprises:
Fixed head, described fixed head offers at least one pair of regulating tank, and described regulating tank is positioned on horizontal direction and/or vertical direction; And
Web member, is connected in the horizontal direction rotationally with described adjusting portion.
7. according to claim 6 based on microscopical cell point device for mark, it is characterized in that, the part that described web member is connected with described adjusting portion carries out locking by pin.
8. according to claim 5 based on microscopical cell point device for mark, it is characterized in that, described adjusting portion comprises:
First regulating part, described first regulating part is provided with slide rail on the length direction along described adjusting portion; And
Second regulating part, described second regulating part is provided with slide block on the length direction along described adjusting portion, and described slide block slides in described slide rail, and described slide block is provided with securing member.
9. according to claim 8 based on microscopical cell point device for mark, it is characterized in that, described slide block is arranged on slider carrier, and described slider carrier is connected with described second regulating part by screw.
10. according to claim 8 based on microscopical cell point device for mark, it is characterized in that, the end of described slide rail is provided with block piece and locating part.
11. according to any one of claim 5-10 based on microscopical cell point device for mark, it is characterized in that, described some mark portion comprises:
Casting die, is arranged on one end away from described fixed part of described adjusting portion by elastomeric element; And
Point tender, to be arranged on described casting die perpendicular to the direction of described objective table, is hollow structure.
12. 1 kinds of microscopes, is characterized in that, comprise described in any one of claim 5-11 based on microscopical cell point device for mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420556286.7U CN204085982U (en) | 2014-09-25 | 2014-09-25 | A kind of based on microscopical cell point device for mark and microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420556286.7U CN204085982U (en) | 2014-09-25 | 2014-09-25 | A kind of based on microscopical cell point device for mark and microscope |
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| CN204085982U true CN204085982U (en) | 2015-01-07 |
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| CN201420556286.7U Expired - Fee Related CN204085982U (en) | 2014-09-25 | 2014-09-25 | A kind of based on microscopical cell point device for mark and microscope |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198255A (en) * | 2014-09-25 | 2014-12-10 | 中国疾病预防控制中心病毒病预防控制所 | Cell marking device based on microscope and microscope |
-
2014
- 2014-09-25 CN CN201420556286.7U patent/CN204085982U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198255A (en) * | 2014-09-25 | 2014-12-10 | 中国疾病预防控制中心病毒病预防控制所 | Cell marking device based on microscope and microscope |
| CN104198255B (en) * | 2014-09-25 | 2017-03-08 | 中国疾病预防控制中心病毒病预防控制所 | One kind is based on microscopical cell point device for mark and microscope |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150107 Termination date: 20190925 |
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| CF01 | Termination of patent right due to non-payment of annual fee |